ORF
Description Cytoplasmic protein of unknown function; potential Cdc28p substrate; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P); YKR078W has a paralog, VPS5, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0.1 0.07 0.05 0 0 0 0 0 0
Cytoplasm 0.94 0.98 0.99 0.99 0.95 0.96 0.85 0.91 0.69 0.8 0.99 0.96 0.98 0.97 0.91 0.95 0.86 0.89 0.91 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.09 0 0 0 0.05 0 0.2 0.12 0.49 0.33 0 0 0 0 0 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Bud 1 0 1 0 2 3 2 0 3 2 0 0 1 0 0 0 0 1 1 0
Bud Neck 0 0 0 2 1 7 3 4 3 1 1 0 1 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cell Periphery 0 3 0 2 0 0 2 0 0 3 3 35 24 17 0 0 0 0 0 0
Cytoplasm 136 160 149 242 214 327 179 268 135 182 172 334 342 343 130 156 31 23 73 80
Endoplasmic Reticulum 1 1 0 2 0 2 1 1 2 0 1 23 11 20 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 1 0 2 1 2 1 0 0 0 2 0
Golgi 1 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0
Mitochondria 13 0 1 0 11 11 43 35 96 75 0 3 3 5 2 1 1 0 0 0
Nucleus 0 0 1 0 4 3 3 1 1 4 1 1 1 1 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 3 4 0 7 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 0 1 2 1 0 1 1 3 0 11 7 2 2 1 1 0 0 2
Unique Cell Count 144 163 150 244 225 339 211 295 195 228 173 349 349 352 143 164 37 26 81 85
Labelled Cell Count 153 165 152 249 235 355 238 315 241 278 178 411 391 391 143 164 37 26 81 85


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 5.8 5.5 6.0 4.7 4.8 4.0 4.1 3.8 3.8 5.7 7.8 7.5 6.7 4.7 5.7
Std Deviation (1e-4) 0.7 0.9 1.3 1.7 1.3 1.3 1.1 0.7 1.1 0.9 1.3 1.2 1.3 1.2 0.7 1.6
Intensity Change (Log2)


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ykr078w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available