ORF | |
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Description | Cytoplasmic protein of unknown function; potential Cdc28p substrate; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P); YKR078W has a paralog, VPS5, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | – | 0 | 0 | 1 | 1 | – | 0 | 0 | 0 | 0 | – | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 1 | 0 | – | 1 | 0 | 2 | 3 | – | 2 | 0 | 3 | 2 | – | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 |
Bud Neck | 0 | 0 | – | 0 | 2 | 1 | 7 | – | 3 | 4 | 3 | 1 | – | – | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 3 | – | 0 | 2 | 0 | 0 | – | 2 | 0 | 0 | 3 | – | – | 3 | 35 | 24 | 17 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 136 | 160 | – | 149 | 242 | 214 | 327 | – | 179 | 268 | 135 | 182 | – | – | 172 | 334 | 342 | 343 | 130 | 156 | 31 | 23 | 73 | 80 |
Endoplasmic Reticulum | 1 | 1 | – | 0 | 2 | 0 | 2 | – | 1 | 1 | 2 | 0 | – | – | 1 | 23 | 11 | 20 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | – | – | 0 | 2 | 1 | 2 | 1 | 0 | 0 | 0 | 2 | 0 |
Golgi | 1 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 13 | 0 | – | 1 | 0 | 11 | 11 | – | 43 | 35 | 96 | 75 | – | – | 0 | 3 | 3 | 5 | 2 | 1 | 1 | 0 | 0 | 0 |
Nucleus | 0 | 0 | – | 1 | 0 | 4 | 3 | – | 3 | 1 | 1 | 4 | – | – | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 3 | 4 | 0 | 7 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 1 | – | 0 | 1 | 2 | 1 | – | 0 | 1 | 1 | 3 | – | – | 0 | 11 | 7 | 2 | 2 | 1 | 1 | 0 | 0 | 2 |
Unique Cell Count | 144 | 163 | 150 | 244 | 225 | 339 | 211 | 295 | 195 | 228 | 173 | 349 | 349 | 352 | 143 | 164 | 37 | 26 | 81 | 85 | ||||
Labelled Cell Count | 153 | 165 | 152 | 249 | 235 | 355 | 238 | 315 | 241 | 278 | 178 | 411 | 391 | 391 | 143 | 164 | 37 | 26 | 81 | 85 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | 4.4 | 5.8 | – | 5.5 | 6.0 | 4.7 | 4.8 | – | 4.0 | 4.1 | 3.8 | 3.8 | – | – | 5.7 | 7.8 | 7.5 | 6.7 | – | 4.7 | 5.7 |
Std Deviation (1e-4) | 0.7 | 0.9 | – | 1.3 | 1.7 | 1.3 | 1.3 | – | 1.1 | 0.7 | 1.1 | 0.9 | – | – | 1.3 | 1.2 | 1.3 | 1.2 | – | 0.7 | 1.6 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
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Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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