| ORF | |
|---|---|
| Description | Cytoplasmic protein of unknown function; potential Cdc28p substrate; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P); YKR078W has a paralog, VPS5, that arose from the whole genome duplication |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | 0 | 0 | – | 0 | 0 | 1 | 1 | – | 0 | 0 | 0 | 0 | – | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Bud | 1 | 0 | – | 1 | 0 | 2 | 3 | – | 2 | 0 | 3 | 2 | – | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 |
| Bud Neck | 0 | 0 | – | 0 | 2 | 1 | 7 | – | 3 | 4 | 3 | 1 | – | – | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Bud Site | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
| Cell Periphery | 0 | 3 | – | 0 | 2 | 0 | 0 | – | 2 | 0 | 0 | 3 | – | – | 3 | 35 | 24 | 17 | 0 | 0 | 0 | 0 | 0 | 0 |
| Cytoplasm | 136 | 160 | – | 149 | 242 | 214 | 327 | – | 179 | 268 | 135 | 182 | – | – | 172 | 334 | 342 | 343 | 130 | 156 | 31 | 23 | 73 | 80 |
| Endoplasmic Reticulum | 1 | 1 | – | 0 | 2 | 0 | 2 | – | 1 | 1 | 2 | 0 | – | – | 1 | 23 | 11 | 20 | 0 | 0 | 0 | 0 | 0 | 0 |
| Endosome | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | – | – | 0 | 2 | 1 | 2 | 1 | 0 | 0 | 0 | 2 | 0 |
| Golgi | 1 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| Mitochondria | 13 | 0 | – | 1 | 0 | 11 | 11 | – | 43 | 35 | 96 | 75 | – | – | 0 | 3 | 3 | 5 | 2 | 1 | 1 | 0 | 0 | 0 |
| Nucleus | 0 | 0 | – | 1 | 0 | 4 | 3 | – | 3 | 1 | 1 | 4 | – | – | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| Nuclear Periphery | 1 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
| Nucleolus | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Peroxisomes | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| SpindlePole | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 3 | 4 | 0 | 7 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Vac/Vac Membrane | 0 | 1 | – | 0 | 1 | 2 | 1 | – | 0 | 1 | 1 | 3 | – | – | 0 | 11 | 7 | 2 | 2 | 1 | 1 | 0 | 0 | 2 |
| Unique Cell Count | 144 | 163 | 150 | 244 | 225 | 339 | 211 | 295 | 195 | 228 | 173 | 349 | 349 | 352 | 143 | 164 | 37 | 26 | 81 | 85 | ||||
| Labelled Cell Count | 153 | 165 | 152 | 249 | 235 | 355 | 238 | 315 | 241 | 278 | 178 | 411 | 391 | 391 | 143 | 164 | 37 | 26 | 81 | 85 | ||||
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | 4.4 | 5.8 | – | 5.5 | 6.0 | 4.7 | 4.8 | – | 4.0 | 4.1 | 3.8 | 3.8 | – | – | 5.7 | 7.8 | 7.5 | 6.7 | – | 4.7 | 5.7 |
| Std Deviation (1e-4) | 0.7 | 0.9 | – | 1.3 | 1.7 | 1.3 | 1.3 | – | 1.1 | 0.7 | 1.1 | 0.9 | – | – | 1.3 | 1.2 | 1.3 | 1.2 | – | 0.7 | 1.6 |
| Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
External localization resources
Endocytosis
| Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
|---|---|---|---|---|
| 37℃ | ||||
| RT |
Cell Cycle Omics
CYCLoPs (Ykr078w-GFP)
| Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
|---|
| Gene | Images |
|---|
| Gene | Images |
|---|
Images are not yet available
Images are not yet available
