Standard name | |
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Human Ortholog | |
Description | Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 3 | 4 | 0 | 2 | 0 | 3 | 4 | 2 | 1 | 0 | 0 | 2 |
Bud | 3 | 3 | 8 | 6 | 7 | 14 | 32 | 43 | – | 42 | 38 | 35 | 1 | 1 | 1 | 14 | 11 | 7 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 1 | 0 | 0 | 2 | 2 | 2 | 0 | 4 | – | 2 | 2 | 1 | 0 | 1 | 0 | 2 | 4 | 1 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 2 | 0 | 0 | 0 | 3 | 6 | 7 | – | 13 | 13 | 10 | 0 | 0 | 0 | 2 | 3 | 1 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 6 | 5 | 13 | 22 | 15 | 13 | 13 | 18 | – | 14 | 4 | 7 | 2 | 4 | 4 | 57 | 67 | 36 | 0 | 0 | 0 | 0 | 1 | 0 |
Endoplasmic Reticulum | 0 | 4 | 0 | 1 | 0 | 0 | 1 | 0 | – | 0 | 0 | 1 | 0 | 0 | 1 | 10 | 12 | 8 | 0 | 0 | 0 | 0 | 2 | 0 |
Endosome | 293 | 41 | 175 | 87 | 196 | 61 | 78 | 75 | – | 25 | 13 | 42 | 33 | 30 | 61 | 278 | 340 | 189 | 395 | 178 | 240 | 105 | 137 | 128 |
Golgi | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 1 | 2 | 6 | 4 | 0 | 0 | 3 | 12 |
Mitochondria | 107 | 139 | 54 | 44 | 51 | 199 | 194 | 211 | – | 203 | 228 | 212 | 7 | 9 | 2 | 65 | 53 | 29 | 7 | 11 | 1 | 3 | 7 | 2 |
Nucleus | 3 | 2 | 0 | 0 | 1 | 1 | 3 | 5 | – | 0 | 1 | 5 | 0 | 0 | 1 | 1 | 4 | 3 | 0 | 2 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 7 | 11 | 1 | 1 | 0 | 0 | 1 | 2 | – | 0 | 0 | 1 | 2 | 3 | 3 | 3 | 5 | 5 | 1 | 2 | 0 | 0 | 0 | 0 |
Nucleolus | 56 | 34 | 34 | 27 | 29 | 42 | 39 | 49 | – | 30 | 33 | 34 | 2 | 7 | 9 | 16 | 32 | 26 | 4 | 2 | 2 | 2 | 0 | 0 |
Peroxisomes | 108 | 20 | 179 | 101 | 190 | 139 | 240 | 165 | – | 140 | 93 | 142 | 1 | 4 | 3 | 118 | 159 | 96 | 26 | 13 | 46 | 8 | 12 | 15 |
SpindlePole | 179 | 50 | 148 | 103 | 138 | 135 | 216 | 231 | – | 173 | 129 | 161 | 29 | 50 | 60 | 275 | 338 | 178 | 50 | 27 | 44 | 19 | 10 | 8 |
Vac/Vac Membrane | 5 | 8 | 4 | 0 | 4 | 2 | 3 | 4 | – | 1 | 1 | 0 | 0 | 1 | 5 | 9 | 19 | 6 | 4 | 2 | 0 | 1 | 2 | 0 |
Unique Cell Count | 459 | 231 | 344 | 239 | 352 | 416 | 516 | 533 | 439 | 373 | 422 | 61 | 88 | 107 | 559 | 670 | 379 | 505 | 255 | 344 | 144 | 184 | 174 | |
Labelled Cell Count | 768 | 321 | 616 | 394 | 633 | 612 | 826 | 814 | 643 | 555 | 651 | 80 | 116 | 151 | 852 | 1048 | 592 | 505 | 255 | 344 | 144 | 184 | 174 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.3 | 5.2 | 4.7 | 5.0 | 4.8 | 3.8 | 3.7 | 3.9 | – | 3.4 | 3.4 | 3.5 | 5.4 | 5.6 | 6.1 | 4.9 | 5.5 | 5.8 | 5.2 | 6.4 | 6.7 |
Std Deviation (1e-4) | 0.8 | 0.7 | 1.4 | 2.0 | 1.3 | 1.3 | 1.3 | 1.2 | – | 1.1 | 0.9 | 0.9 | 0.8 | 0.7 | 0.6 | 1.1 | 1.4 | 1.4 | 0.9 | 1.2 | 1.5 |
Intensity Change (Log2) | – | – | – | 0.07 | 0.01 | -0.33 | -0.35 | -0.27 | – | -0.47 | -0.49 | -0.45 | 0.19 | 0.24 | 0.35 | 0.04 | 0.21 | 0.29 | 0.14 | 0.44 | 0.49 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0.1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0.2 | -0.8 | -0.5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2.7 | 0.3 | -0.5 | -1.1 | -0.3 | – | -0.4 | -2.4 | -1.8 | 0 | 0 | 0 | 3.5 | 3.5 | 3.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -3.5 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0.5 | -2.8 | 1.1 | -0.3 | 0 | -0.3 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.9 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | -0.8 | -1.3 | -3.8 | -1.8 | -3.8 | -3.4 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0.5 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | -1.7 | -0.6 | -0.5 | -4.5 | -3.1 | -1.5 |
Peroxisomes | -2.3 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | -7.3 | -8.0 | -9.1 | -9.6 | -9.1 | -7.4 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0.7 | 2.3 | 2.4 | 1.8 | 2.2 | 1.1 |
Vacuole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.7 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0309 | 0.0142 | 0.0071 | 0.0031 | 0 | 0.0001 | 0.0008 | 0.0002 | 0.001 | 0.0005 | 0.0007 | 0.0001 | 0.0008 | 0 | 0.0002 | 0 | 0.0124 | 0.0001 |
Bud | 0.0022 | 0.0002 | 0.0005 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0128 | 0 | 0 | 0.0003 | 0 | 0.0003 | 0 | 0.0002 | 0 |
Bud Neck | 0.0015 | 0.0012 | 0.0005 | 0.0006 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0002 | 0.0017 | 0 | 0.0001 | 0.0001 | 0.0003 | 0.0001 |
Bud Periphery | 0.0035 | 0.0002 | 0.0006 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0052 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0002 | 0 |
Bud Site | 0.0776 | 0.0031 | 0.0023 | 0.0015 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0031 | 0.0001 | 0.0001 | 0.0041 | 0 | 0.0004 | 0.0001 | 0.0003 | 0 |
Cell Periphery | 0.0046 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.013 | 0.0025 | 0.0269 | 0.0007 | 0.0005 | 0.0026 | 0.0018 | 0.0004 | 0.0004 | 0.0022 | 0.0026 | 0.0002 | 0.0016 | 0.0001 | 0.0003 | 0.0009 | 0.004 | 0.0003 |
Cytoplasmic Foci | 0.2456 | 0.1917 | 0.1523 | 0.1697 | 0.0907 | 0.1565 | 0.1885 | 0.2314 | 0.2276 | 0.1436 | 0.1562 | 0.1402 | 0.2078 | 0.1093 | 0.2804 | 0.177 | 0.1245 | 0.2169 |
Eisosomes | 0.0017 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0045 | 0.0003 | 0.0112 | 0.0001 | 0.0006 | 0.0002 | 0.0007 | 0.0001 | 0.0001 | 0.0001 | 0.0005 | 0.0001 | 0.0006 | 0 | 0 | 0.0001 | 0.0038 | 0 |
Endosome | 0.07 | 0.2261 | 0.1943 | 0.1857 | 0.115 | 0.1717 | 0.127 | 0.0918 | 0.084 | 0.2378 | 0.0368 | 0.0913 | 0.4131 | 0.0416 | 0.0513 | 0.3572 | 0.1727 | 0.0703 |
Golgi | 0.0252 | 0.046 | 0.0503 | 0.07 | 0.0242 | 0.0264 | 0.0319 | 0.0222 | 0.0361 | 0.0507 | 0.0196 | 0.0324 | 0.1722 | 0.0151 | 0.0174 | 0.0522 | 0.0544 | 0.0302 |
Lipid Particles | 0.3596 | 0.3377 | 0.3222 | 0.334 | 0.6772 | 0.4148 | 0.3447 | 0.3724 | 0.3457 | 0.2871 | 0.4021 | 0.5396 | 0.13 | 0.3971 | 0.3924 | 0.0954 | 0.4565 | 0.4605 |
Mitochondria | 0.0159 | 0.0024 | 0.0675 | 0.0025 | 0.0042 | 0.0009 | 0.0012 | 0.0006 | 0.0016 | 0.0167 | 0.0016 | 0.0036 | 0.0152 | 0.0007 | 0.0007 | 0.0013 | 0.0027 | 0.0008 |
None | 0.0053 | 0.0006 | 0.0066 | 0 | 0.0002 | 0.0208 | 0.0007 | 0.0001 | 0.0006 | 0.0019 | 0.0031 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.008 | 0.0004 |
Nuclear Periphery | 0.0015 | 0.0006 | 0.0029 | 0.0001 | 0.0005 | 0.0002 | 0.018 | 0.0001 | 0.0001 | 0.0003 | 0.0014 | 0.0001 | 0.0005 | 0 | 0 | 0.0001 | 0.0139 | 0.0001 |
Nucleolus | 0.0008 | 0.0009 | 0.0046 | 0.0003 | 0.001 | 0.0002 | 0.0004 | 0.0002 | 0.0002 | 0.0065 | 0.0001 | 0.0005 | 0.0016 | 0.0001 | 0.0003 | 0.0002 | 0.0004 | 0.0001 |
Nucleus | 0.0016 | 0.0002 | 0.0011 | 0.0001 | 0.0004 | 0.0001 | 0.0237 | 0 | 0 | 0.0043 | 0.0001 | 0.0001 | 0.0007 | 0 | 0 | 0.0001 | 0.0009 | 0 |
Peroxisomes | 0.1265 | 0.1556 | 0.1303 | 0.2285 | 0.0718 | 0.1983 | 0.2242 | 0.2746 | 0.2887 | 0.2201 | 0.3694 | 0.1867 | 0.0331 | 0.4344 | 0.2514 | 0.3067 | 0.1305 | 0.2165 |
Punctate Nuclear | 0.0029 | 0.0014 | 0.0127 | 0.001 | 0.0019 | 0.0019 | 0.0332 | 0.003 | 0.0125 | 0.0033 | 0.0052 | 0.0021 | 0.0033 | 0.001 | 0.0041 | 0.0004 | 0.0113 | 0.0029 |
Vacuole | 0.0045 | 0.0119 | 0.0036 | 0.0013 | 0.0086 | 0.0038 | 0.0012 | 0.0023 | 0.001 | 0.0018 | 0.0002 | 0.0019 | 0.0099 | 0.0003 | 0.0003 | 0.0064 | 0.0014 | 0.0005 |
Vacuole Periphery | 0.001 | 0.0027 | 0.0022 | 0.0005 | 0.0023 | 0.0012 | 0.0017 | 0.0004 | 0.0003 | 0.0013 | 0.0003 | 0.001 | 0.0025 | 0.0001 | 0.0001 | 0.0015 | 0.0018 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 5.1925 | 7.4784 | 8.2283 | 12.1419 | 8.6024 | 5.4779 | 9.8429 | 11.1748 | 11.9419 | 12.2079 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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