Standard name
Human Ortholog
Description Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.06 0.08 0.1 0.1 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.09 0 0 0 0 0 0 0 0 0.05 0 0.1 0.1 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.64 0.18 0.51 0.36 0.56 0.15 0.15 0.14 0.06 0 0.1 0.54 0.34 0.57 0.5 0.51 0.5 0.78 0.7 0.7 0.73 0.75 0.74
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07
Mitochondria 0.23 0.6 0.16 0.18 0.14 0.48 0.38 0.4 0.46 0.61 0.5 0.11 0.1 0 0.12 0.08 0.08 0 0.05 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.12 0.15 0.1 0.11 0.08 0.1 0.08 0.09 0.07 0.09 0.08 0 0.08 0.08 0 0.05 0.07 0 0 0 0 0 0
Peroxisomes 0.24 0.09 0.52 0.42 0.54 0.33 0.47 0.31 0.32 0.25 0.34 0 0.05 0 0.21 0.24 0.25 0.05 0.05 0.14 0.06 0.07 0.09
SpindlePole 0.39 0.22 0.43 0.43 0.39 0.32 0.42 0.43 0.39 0.35 0.38 0.48 0.57 0.56 0.49 0.5 0.47 0.1 0.11 0.13 0.13 0.06 0.05
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 0 0 0 0 0 0 0 0 0 3 4 0 2 0 3 4 2 1 0 0 2
Bud 3 3 8 6 7 14 32 43 42 38 35 1 1 1 14 11 7 0 0 0 0 0 0
Bud Neck 1 0 0 2 2 2 0 4 2 2 1 0 1 0 2 4 1 0 1 0 0 0 0
Bud Site 0 2 0 0 0 3 6 7 13 13 10 0 0 0 2 3 1
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0
Cytoplasm 6 5 13 22 15 13 13 18 14 4 7 2 4 4 57 67 36 0 0 0 0 1 0
Endoplasmic Reticulum 0 4 0 1 0 0 1 0 0 0 1 0 0 1 10 12 8 0 0 0 0 2 0
Endosome 293 41 175 87 196 61 78 75 25 13 42 33 30 61 278 340 189 395 178 240 105 137 128
Golgi 0 0 0 0 0 1 0 0 0 0 0 0 2 1 0 1 2 6 4 0 0 3 12
Mitochondria 107 139 54 44 51 199 194 211 203 228 212 7 9 2 65 53 29 7 11 1 3 7 2
Nucleus 3 2 0 0 1 1 3 5 0 1 5 0 0 1 1 4 3 0 2 0 0 1 0
Nuclear Periphery 7 11 1 1 0 0 1 2 0 0 1 2 3 3 3 5 5 1 2 0 0 0 0
Nucleolus 56 34 34 27 29 42 39 49 30 33 34 2 7 9 16 32 26 4 2 2 2 0 0
Peroxisomes 108 20 179 101 190 139 240 165 140 93 142 1 4 3 118 159 96 26 13 46 8 12 15
SpindlePole 179 50 148 103 138 135 216 231 173 129 161 29 50 60 275 338 178 50 27 44 19 10 8
Vac/Vac Membrane 5 8 4 0 4 2 3 4 1 1 0 0 1 5 9 19 6 4 2 0 1 2 0
Unique Cell Count 459 231 344 239 352 416 516 533 439 373 422 61 88 107 559 670 379 505 255 344 144 184 174
Labelled Cell Count 768 321 616 394 633 612 826 814 643 555 651 80 116 151 852 1048 592 505 255 344 144 184 174


Endosome

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 5.2 4.7 5.0 4.8 3.8 3.7 3.9 3.4 3.4 3.5 5.4 5.6 6.1 4.9 5.5 5.8 5.2 6.4 6.7
Std Deviation (1e-4) 0.8 0.7 1.4 2.0 1.3 1.3 1.3 1.2 1.1 0.9 0.9 0.8 0.7 0.6 1.1 1.4 1.4 0.9 1.2 1.5
Intensity Change (Log2) 0.07 0.01 -0.33 -0.35 -0.27 -0.47 -0.49 -0.45 0.19 0.24 0.35 0.04 0.21 0.29 0.14 0.44 0.49


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0.1 0 0 0 0 0 0 0 0 0 0 0.2 -0.8 -0.5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.7 0.3 -0.5 -1.1 -0.3 -0.4 -2.4 -1.8 0 0 0 3.5 3.5 3.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -3.5 0 0 0 0 0 0 0 0.5 -2.8 1.1 -0.3 0 -0.3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.9 0 0 0 0 0 0 0 -0.8 -1.3 -3.8 -1.8 -3.8 -3.4
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.5 0 0 0 0 0 0 0 -1.7 -0.6 -0.5 -4.5 -3.1 -1.5
Peroxisomes -2.3 0 0 0 0 0 0 0 -7.3 -8.0 -9.1 -9.6 -9.1 -7.4
SpindlePole 0 0 0 0 0 0 0 0 0.7 2.3 2.4 1.8 2.2 1.1
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 1.7 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin 0.0309 0.0142 0.0071 0.0031 0 0.0001 0.0008 0.0002 0.001 0.0005 0.0007 0.0001 0.0008 0 0.0002 0 0.0124 0.0001
Bud 0.0022 0.0002 0.0005 0.0002 0 0 0 0 0 0.0128 0 0 0.0003 0 0.0003 0 0.0002 0
Bud Neck 0.0015 0.0012 0.0005 0.0006 0.0003 0.0001 0.0002 0.0001 0.0001 0.0004 0 0.0002 0.0017 0 0.0001 0.0001 0.0003 0.0001
Bud Periphery 0.0035 0.0002 0.0006 0.0001 0.0001 0 0 0 0 0.0052 0 0 0.0003 0 0.0001 0 0.0002 0
Bud Site 0.0776 0.0031 0.0023 0.0015 0.0002 0.0001 0.0001 0.0001 0.0001 0.0031 0.0001 0.0001 0.0041 0 0.0004 0.0001 0.0003 0
Cell Periphery 0.0046 0.0003 0.0002 0.0001 0.0001 0 0 0 0 0.0002 0 0 0.0002 0 0 0 0 0
Cytoplasm 0.013 0.0025 0.0269 0.0007 0.0005 0.0026 0.0018 0.0004 0.0004 0.0022 0.0026 0.0002 0.0016 0.0001 0.0003 0.0009 0.004 0.0003
Cytoplasmic Foci 0.2456 0.1917 0.1523 0.1697 0.0907 0.1565 0.1885 0.2314 0.2276 0.1436 0.1562 0.1402 0.2078 0.1093 0.2804 0.177 0.1245 0.2169
Eisosomes 0.0017 0.0001 0.0001 0 0 0 0 0 0 0 0 0 0 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0045 0.0003 0.0112 0.0001 0.0006 0.0002 0.0007 0.0001 0.0001 0.0001 0.0005 0.0001 0.0006 0 0 0.0001 0.0038 0
Endosome 0.07 0.2261 0.1943 0.1857 0.115 0.1717 0.127 0.0918 0.084 0.2378 0.0368 0.0913 0.4131 0.0416 0.0513 0.3572 0.1727 0.0703
Golgi 0.0252 0.046 0.0503 0.07 0.0242 0.0264 0.0319 0.0222 0.0361 0.0507 0.0196 0.0324 0.1722 0.0151 0.0174 0.0522 0.0544 0.0302
Lipid Particles 0.3596 0.3377 0.3222 0.334 0.6772 0.4148 0.3447 0.3724 0.3457 0.2871 0.4021 0.5396 0.13 0.3971 0.3924 0.0954 0.4565 0.4605
Mitochondria 0.0159 0.0024 0.0675 0.0025 0.0042 0.0009 0.0012 0.0006 0.0016 0.0167 0.0016 0.0036 0.0152 0.0007 0.0007 0.0013 0.0027 0.0008
None 0.0053 0.0006 0.0066 0 0.0002 0.0208 0.0007 0.0001 0.0006 0.0019 0.0031 0.0001 0.0003 0 0.0001 0.0001 0.008 0.0004
Nuclear Periphery 0.0015 0.0006 0.0029 0.0001 0.0005 0.0002 0.018 0.0001 0.0001 0.0003 0.0014 0.0001 0.0005 0 0 0.0001 0.0139 0.0001
Nucleolus 0.0008 0.0009 0.0046 0.0003 0.001 0.0002 0.0004 0.0002 0.0002 0.0065 0.0001 0.0005 0.0016 0.0001 0.0003 0.0002 0.0004 0.0001
Nucleus 0.0016 0.0002 0.0011 0.0001 0.0004 0.0001 0.0237 0 0 0.0043 0.0001 0.0001 0.0007 0 0 0.0001 0.0009 0
Peroxisomes 0.1265 0.1556 0.1303 0.2285 0.0718 0.1983 0.2242 0.2746 0.2887 0.2201 0.3694 0.1867 0.0331 0.4344 0.2514 0.3067 0.1305 0.2165
Punctate Nuclear 0.0029 0.0014 0.0127 0.001 0.0019 0.0019 0.0332 0.003 0.0125 0.0033 0.0052 0.0021 0.0033 0.001 0.0041 0.0004 0.0113 0.0029
Vacuole 0.0045 0.0119 0.0036 0.0013 0.0086 0.0038 0.0012 0.0023 0.001 0.0018 0.0002 0.0019 0.0099 0.0003 0.0003 0.0064 0.0014 0.0005
Vacuole Periphery 0.001 0.0027 0.0022 0.0005 0.0023 0.0012 0.0017 0.0004 0.0003 0.0013 0.0003 0.001 0.0025 0.0001 0.0001 0.0015 0.0018 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.1925 7.4784 8.2283 12.1419 8.6024 5.4779 9.8429 11.1748 11.9419 12.2079
Translational Efficiency

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2158 1124 131 1331 565 1356 453 927 2723 2480 584 2258

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 761.26 686.25 937.02 852.75 608.92 748.10 838.25 831.50 729.65 720.07 860.41 844.03
Standard Deviation 84.65 100.98 113.54 116.52 78.10 94.31 125.72 113.44 103.73 102.14 129.80 115.74
Intensity Change Log 2 -0.149655 0.299691 0.163734 0.296978 0.461128 0.449464 0.066032 0.373673 0.297741

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000355 0.000450 0.002192 0.002030 0.001582 0.000213 0.000514 0.000570 0.000610 0.000321 0.000891 0.001430
Bud Neck 0.003065 0.007633 0.013775 0.004655 0.003176 0.004443 0.007365 0.004219 0.003088 0.005889 0.008803 0.004476
Bud Site 0.002534 0.004239 0.007300 0.012607 0.000944 0.001526 0.005079 0.005025 0.002204 0.002756 0.005577 0.009494
Cell Periphery 0.000024 0.000097 0.000118 0.000044 0.000096 0.000042 0.000051 0.000034 0.000039 0.000067 0.000066 0.000040
Cytoplasm 0.001633 0.009470 0.001565 0.005071 0.001844 0.002813 0.001680 0.002929 0.001677 0.005830 0.001654 0.004192
Cytoplasmic Foci 0.126711 0.166323 0.190013* 0.214479* 0.115178 0.118590 0.153454 0.187166* 0.124318 0.140224 0.161655 0.203266*
Eisosomes 0.000018 0.000051 0.000071 0.000027 0.000079 0.000036 0.000045 0.000026 0.000030 0.000043 0.000051 0.000027
Endoplasmic Reticulum 0.000104 0.000138 0.000872 0.000217 0.000040 0.000084 0.000337 0.000197 0.000091 0.000109 0.000457 0.000209
Endosome 0.612419* 0.487801* 0.371137* 0.454773* 0.285816* 0.575457* 0.506138* 0.490406* 0.544652* 0.535729* 0.475855* 0.469402*
Golgi 0.026649 0.079160 0.036157 0.045059 0.116200* 0.083894 0.025028 0.042327 0.045230 0.081748 0.027524 0.043937
Lipid Particles 0.020152 0.050930 0.061292 0.018907 0.085490 0.057240 0.047575 0.040118 0.033709 0.054380 0.050652 0.027615
Mitochondria 0.005359 0.013746 0.002418 0.006363 0.024024 0.015769 0.003731 0.003340 0.009232 0.014852 0.003436 0.005122
Mitotic Spindle 0.019033 0.003755 0.053240 0.046109 0.000948 0.001712 0.032182 0.026718 0.015280 0.002638 0.036906 0.038148
None 0.000041 0.001186 0.000029 0.000027 0.000614 0.000240 0.000052 0.000078 0.000160 0.000669 0.000047 0.000048
Nuclear Periphery 0.000145 0.000094 0.000682 0.000263 0.000023 0.000087 0.000437 0.000110 0.000120 0.000091 0.000492 0.000200
Nuclear Periphery Foci 0.005645 0.002454 0.027223 0.004357 0.000532 0.001449 0.024765 0.008054 0.004584 0.001904 0.025316 0.005875
Nucleolus 0.001061 0.001705 0.005366 0.000553 0.001179 0.000345 0.002772 0.001705 0.001086 0.000961 0.003354 0.001026
Nucleus 0.000468 0.002749 0.004751 0.000280 0.001518 0.000313 0.002092 0.000841 0.000686 0.001417 0.002688 0.000510
Peroxisomes 0.166244* 0.158252* 0.193103* 0.159725* 0.359858* 0.130846* 0.171404* 0.174394* 0.206417* 0.143267* 0.176271* 0.165747*
Vacuole 0.007305 0.007611 0.025890 0.022073 0.000566 0.003287 0.013982 0.009843 0.005907 0.005247 0.016653 0.017052
Vacuole Periphery 0.001033 0.002157 0.002807 0.002382 0.000292 0.001615 0.001316 0.001899 0.000880 0.001861 0.001651 0.002184

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.54 -2.04 -4.50 -4.52 0.14 2.54 1.75 1.78 -2.24 0.02 1.65 -1.12 -3.09 -5.18 -1.54
Bud Neck -3.42 -3.74 -2.96 2.00 3.09 -0.97 -3.24 -0.91 0.15 2.48 -3.22 -5.42 -2.72 1.38 3.85
Bud Site -2.50 -3.50 -7.73 -6.09 -3.14 -1.19 -4.57 -3.98 -3.43 0.10 -1.24 -4.46 -8.27 -7.47 -3.52
Cell Periphery -5.80 -4.26 -5.68 4.19 3.33 2.66 2.10 3.02 0.96 3.13 -3.62 -3.80 -0.32 3.92 4.17
Cytoplasm -4.90 0.37 -4.30 2.01 -4.09 -0.89 -0.07 -1.27 -0.49 -1.44 -4.57 0.09 -4.19 0.89 -4.10
Cytoplasmic Foci -7.67 -4.34 -15.12 -6.68 -1.54 -0.58 -4.09 -9.45 -10.42 -3.57 -4.40 -5.03 -17.29 -13.18 -5.32
Eisosomes -9.20 -6.22 -4.90 6.47 5.12 3.22 2.17 3.95 2.53 4.25 -3.37 -4.52 1.18 6.21 6.11
Endoplasmic Reticulum -0.93 -3.22 -1.82 -1.46 2.65 -2.21 -5.53 -3.29 -2.34 2.28 -0.44 -5.41 -2.76 -2.61 3.32
Endosome 11.53 10.34 15.97 2.99 -3.39 -20.50 -11.95 -13.46 6.86 0.81 0.91 5.19 8.96 8.01 0.48
Golgi -14.89 -2.42 -7.82 8.76 -1.91 4.61 14.43 11.46 10.43 -6.32 -12.49 7.85 0.81 13.53 -7.44
Lipid Particles -7.27 -3.25 0.67 7.54 3.37 3.46 4.17 5.63 3.17 1.10 -6.05 -2.99 2.34 8.04 4.16
Mitochondria -4.47 3.11 -0.65 3.57 -2.96 1.99 5.28 5.43 6.56 0.39 -3.52 4.79 3.28 6.73 -1.61
Mitotic Spindle 7.67 -2.28 -6.67 -11.11 0.32 -2.26 -6.02 -7.51 -7.27 0.88 8.67 -4.02 -7.81 -13.47 -0.30
None -2.21 1.44 1.43 2.25 -0.17 1.05 1.61 1.54 1.16 -0.55 -1.97 1.56 1.57 2.51 0.07
Nuclear Periphery 1.74 -4.01 -2.23 -3.31 2.81 -1.56 -4.88 -5.72 -0.67 3.76 1.13 -4.89 -2.34 -3.11 3.53
Nuclear Periphery Foci 2.33 -2.42 1.36 -1.25 2.63 -1.58 -6.37 -4.83 -4.12 3.91 3.01 -5.70 -1.17 -4.25 5.32
Nucleolus -1.04 -1.23 1.27 2.06 1.41 1.21 -1.58 -0.66 -3.30 1.27 0.72 -2.30 0.21 -0.50 2.40
Nucleus -2.86 -1.45 0.91 3.12 1.51 1.16 -0.48 0.59 -1.69 1.47 -1.57 -2.15 0.54 2.21 2.38
Peroxisomes 1.08 -1.54 1.08 -0.00 1.96 16.63* 11.90 12.69 -4.75 -0.33 10.16 3.22 6.29 -3.46 1.11
Vacuole 0.03 -2.80 -7.77 -7.51 0.06 -5.47 -6.11 -7.62 -5.17 1.99 1.16 -4.75 -8.59 -9.48 -0.41
Vacuole Periphery -1.76 -2.56 -2.80 -0.32 0.54 -4.40 -3.48 -2.74 -0.42 -0.86 -2.81 -2.56 -3.41 -0.68 -1.15