Standard name
Description Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.06 0.08 0.1 0.1 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.09 0 0 0 0 0 0 0 0 0.05 0 0.1 0.1 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.64 0.18 0.51 0.36 0.56 0.15 0.15 0.14 0.06 0 0.1 0.54 0.34 0.57 0.5 0.51 0.5 0.78 0.7 0.7 0.73 0.75 0.74
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07
Mitochondria 0.23 0.6 0.16 0.18 0.14 0.48 0.38 0.4 0.46 0.61 0.5 0.11 0.1 0 0.12 0.08 0.08 0 0.05 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.12 0.15 0.1 0.11 0.08 0.1 0.08 0.09 0.07 0.09 0.08 0 0.08 0.08 0 0.05 0.07 0 0 0 0 0 0
Peroxisomes 0.24 0.09 0.52 0.42 0.54 0.33 0.47 0.31 0.32 0.25 0.34 0 0.05 0 0.21 0.24 0.25 0.05 0.05 0.14 0.06 0.07 0.09
SpindlePole 0.39 0.22 0.43 0.43 0.39 0.32 0.42 0.43 0.39 0.35 0.38 0.48 0.57 0.56 0.49 0.5 0.47 0.1 0.11 0.13 0.13 0.06 0.05
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 0 0 0 0 0 0 0 0 0 3 4 0 2 0 3 4 2 1 0 0 2
Bud 3 3 8 6 7 14 32 43 42 38 35 1 1 1 14 11 7 0 0 0 0 0 0
Bud Neck 1 0 0 2 2 2 0 4 2 2 1 0 1 0 2 4 1 0 1 0 0 0 0
Bud Site 0 2 0 0 0 3 6 7 13 13 10 0 0 0 2 3 1
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0
Cytoplasm 6 5 13 22 15 13 13 18 14 4 7 2 4 4 57 67 36 0 0 0 0 1 0
Endoplasmic Reticulum 0 4 0 1 0 0 1 0 0 0 1 0 0 1 10 12 8 0 0 0 0 2 0
Endosome 293 41 175 87 196 61 78 75 25 13 42 33 30 61 278 340 189 395 178 240 105 137 128
Golgi 0 0 0 0 0 1 0 0 0 0 0 0 2 1 0 1 2 6 4 0 0 3 12
Mitochondria 107 139 54 44 51 199 194 211 203 228 212 7 9 2 65 53 29 7 11 1 3 7 2
Nucleus 3 2 0 0 1 1 3 5 0 1 5 0 0 1 1 4 3 0 2 0 0 1 0
Nuclear Periphery 7 11 1 1 0 0 1 2 0 0 1 2 3 3 3 5 5 1 2 0 0 0 0
Nucleolus 56 34 34 27 29 42 39 49 30 33 34 2 7 9 16 32 26 4 2 2 2 0 0
Peroxisomes 108 20 179 101 190 139 240 165 140 93 142 1 4 3 118 159 96 26 13 46 8 12 15
SpindlePole 179 50 148 103 138 135 216 231 173 129 161 29 50 60 275 338 178 50 27 44 19 10 8
Vac/Vac Membrane 5 8 4 0 4 2 3 4 1 1 0 0 1 5 9 19 6 4 2 0 1 2 0
Unique Cell Count 459 231 344 239 352 416 516 533 439 373 422 61 88 107 559 670 379 505 255 344 144 184 174
Labelled Cell Count 768 321 616 394 633 612 826 814 643 555 651 80 116 151 852 1048 592 505 255 344 144 184 174


Endosome

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 5.2 4.7 5.0 4.8 3.8 3.7 3.9 3.4 3.4 3.5 5.4 5.6 6.1 4.9 5.5 5.8 5.2 6.4 6.7
Std Deviation (1e-4) 0.8 0.7 1.4 2.0 1.3 1.3 1.3 1.2 1.1 0.9 0.9 0.8 0.7 0.6 1.1 1.4 1.4 0.9 1.2 1.5
Intensity Change (Log2) 0.07 0.01 -0.33 -0.35 -0.27 -0.47 -0.49 -0.45 0.19 0.24 0.35 0.04 0.21 0.29 0.14 0.44 0.49


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0.1 0 0 0 0 0 0 0 0 0 0 0.2 -0.8 -0.5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.7 0.3 -0.5 -1.1 -0.3 -0.4 -2.4 -1.8 0 0 0 3.5 3.5 3.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -3.5 0 0 0 0 0 0 0 0.5 -2.8 1.1 -0.3 0 -0.3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.9 0 0 0 0 0 0 0 -0.8 -1.3 -3.8 -1.8 -3.8 -3.4
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.5 0 0 0 0 0 0 0 -1.7 -0.6 -0.5 -4.5 -3.1 -1.5
Peroxisomes -2.3 0 0 0 0 0 0 0 -7.3 -8.0 -9.1 -9.6 -9.1 -7.4
SpindlePole 0 0 0 0 0 0 0 0 0.7 2.3 2.4 1.8 2.2 1.1
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 1.7 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vps8-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available