Standard name
Human Ortholog
Description GTPase with similarity to translation release factors; together with binding partner Dom34p, facilitates ribosomal subunit dissociation and peptidyl-tRNA release when translation is stalled, particularly in 3' UTRs; genetically implicated in mRNA no-go decay; HBS1 has a paralog, SKI7, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0.05 0 0 0.06 0.06 0.07 0.06 0 0 0 0.06 0 0.05 0 0 0 0 0 0
Cytoplasm 0.97 0.98 0.97 0.96 0.97 0.91 0.83 0.85 0.76 0.83 0.69 0.78 0.97 0.96 0.98 0.95 0.92 0.93 0.93 0.96 0.87 0.88 0.91 0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.07 0.07 0.1 0.24 0.13 0.45 0.19 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0.05 0 0 0.05 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 2 3 5 1 4 0 1 0 1 0 0 0 0 2 0 0 0 0 0
Bud 2 1 0 0 0 1 0 2 1 0 3 0 0 0 0 0 0 1 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 7 7 0 1 3 12 8 5 10 11 11 13 0 5 4 23 11 20 0 0 0 0 0 0
Cytoplasm 452 346 112 110 207 205 221 274 133 150 109 167 34 145 138 394 278 346 441 350 109 33 49 100
Endoplasmic Reticulum 4 6 1 2 3 3 24 10 3 5 2 7 0 4 2 8 11 7 3 2 3 0 0 0
Endosome 1 2 0 0 0 0 0 2 0 0 0 2 0 0 0 0 1 0 2 0 0 0 0 2
Golgi 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 1 1 0 0 0 1
Mitochondria 11 0 0 2 1 15 19 33 42 24 71 41 1 0 0 1 5 3 7 2 1 0 0 0
Nucleus 2 0 2 4 0 1 0 3 1 1 0 1 1 2 2 10 15 5 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 1 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0
Vac/Vac Membrane 2 2 2 1 1 4 6 6 9 8 4 6 0 1 0 15 13 19 4 4 6 0 1 1
Unique Cell Count 465 354 116 115 214 226 265 321 174 181 158 215 35 151 141 416 302 372 474 365 125 38 55 112
Labelled Cell Count 483 366 117 120 216 243 281 340 201 203 200 239 36 158 146 454 335 401 474 365 125 38 55 112


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.6 11.5 10.4 9.6 10.0 8.8 8.0 8.1 7.3 7.6 6.7 7.2 9.7 12.9 11.3 12.4 11.9 11.7 11.6 11.2
Std Deviation (1e-4) 1.3 1.8 1.7 1.9 1.7 2.0 1.3 1.3 1.8 1.6 1.3 1.4 2.2 9.3 2.1 3.2 3.0 3.0 2.4 2.3
Intensity Change (Log2) -0.12 -0.05 -0.24 -0.37 -0.35 -0.5 -0.45 -0.63 -0.53 -0.1 0.31 0.13 0.25 0.2 0.17 0.16 0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 2.6 0 0
Cytoplasm -0.4 0.1 -2.0 -3.6 -3.2 -4.6 -3.6 -5.7 -4.5 0.2 -0.2 0.6 -0.8 -1.6 -1.4
Endoplasmic Reticulum 0 0 0 3.0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 2.8 3.0 3.6 5.7 4.1 8.4 5.0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.2712 5.865 5.1404 4.4678 4.7521 5.204 3.5345 5.1439 4.7613 4.3745 3.89 4.9184 4.5684 6.6009 5.8221 5.3452 4.3997 5.6458
Actin 0.0319 0.0013 0.0155 0.0001 0.001 0.0029 0.017 0.0013 0.016 0.0012 0.0344 0.0032 0.0123 0.0003 0.0043 0.0275 0.0608 0.0115
Bud 0.0033 0.0043 0.0039 0.0003 0.0002 0.008 0.0009 0.0006 0.0006 0.0005 0.0006 0.0011 0.0002 0.0003 0.0003 0.0005 0.0008 0.0002
Bud Neck 0.0049 0.0002 0.0017 0.0003 0.0005 0.0021 0.0011 0.0002 0.0004 0.0002 0.0006 0.0008 0.0006 0.0002 0.0004 0.0006 0.0008 0.0025
Bud Periphery 0.0029 0.0013 0.0027 0.0001 0.0001 0.0032 0.0012 0.0005 0.0003 0.0002 0.0006 0.0076 0.0002 0.0001 0.0003 0.0002 0.001 0.0002
Bud Site 0.0054 0.004 0.0067 0.0001 0.0002 0.001 0.0034 0.0029 0.0009 0.0002 0.0011 0.0006 0.0007 0.0017 0.0007 0.0016 0.0007 0.0002
Cell Periphery 0.0007 0.0002 0.0004 0 0 0.0003 0.0004 0.0002 0.0001 0.0001 0.0002 0.0044 0.0002 0.0001 0.0002 0.0001 0.0001 0.0001
Cytoplasm 0.6794 0.9424 0.8685 0.9132 0.8437 0.8694 0.7501 0.9328 0.8828 0.9016 0.8348 0.866 0.8445 0.9618 0.9112 0.7768 0.705 0.8764
Cytoplasmic Foci 0.0503 0.0089 0.0206 0.0168 0.0247 0.0172 0.0386 0.0125 0.0254 0.0121 0.011 0.0149 0.021 0.0084 0.0087 0.0619 0.0181 0.0123
Eisosomes 0.0003 0 0.0001 0 0 0 0.0001 0 0.0001 0 0.0003 0.0001 0.0001 0 0.0002 0.0001 0.0003 0.0001
Endoplasmic Reticulum 0.0257 0.0087 0.0045 0.0026 0.0028 0.0102 0.0325 0.006 0.0137 0.006 0.0286 0.036 0.0393 0.0073 0.0122 0.003 0.0157 0.0275
Endosome 0.0953 0.0113 0.0323 0.0425 0.0834 0.0367 0.0498 0.0158 0.0248 0.0524 0.0291 0.0275 0.0351 0.0104 0.0247 0.0748 0.0971 0.03
Golgi 0.0166 0.0013 0.0074 0.001 0.0014 0.0051 0.0079 0.0015 0.0035 0.0015 0.0167 0.0032 0.0052 0.001 0.003 0.0086 0.0319 0.0045
Lipid Particles 0.0088 0.0002 0.0021 0 0.0021 0.0018 0.0139 0.0002 0.001 0.0001 0.0134 0.005 0.0054 0.0001 0.0011 0.0097 0.0115 0.0071
Mitochondria 0.0092 0.0012 0.0062 0.0008 0.0008 0.0197 0.0046 0.0005 0.0051 0.0005 0.0073 0.01 0.0015 0.0002 0.0173 0.0014 0.0089 0.0022
None 0.0129 0.0061 0.0108 0.0024 0.0034 0.0083 0.0448 0.0193 0.0091 0.005 0.0025 0.0038 0.0105 0.0034 0.0024 0.0042 0.0027 0.0019
Nuclear Periphery 0.012 0.001 0.0016 0.0008 0.0128 0.0011 0.0043 0.0007 0.0024 0.0013 0.0028 0.0015 0.0089 0.0005 0.003 0.0013 0.0206 0.0096
Nucleolus 0.0004 0.0001 0.0002 0 0.0007 0.0014 0.0004 0 0.0001 0 0.0002 0.0001 0.0003 0 0.0002 0.0001 0.0002 0.0004
Nucleus 0.0075 0.0018 0.002 0.0011 0.0045 0.003 0.0098 0.0008 0.0014 0.001 0.001 0.0009 0.0023 0.0007 0.0012 0.0011 0.0045 0.0026
Peroxisomes 0.01 0.0003 0.0049 0.0001 0.0002 0.0019 0.0058 0.0001 0.001 0.0001 0.0043 0.0037 0.0026 0 0.0006 0.0091 0.0026 0.0021
Punctate Nuclear 0.0048 0.0003 0.0014 0.0001 0.0049 0.0005 0.0058 0.0002 0.0056 0.0001 0.0024 0.0036 0.004 0.0001 0.0027 0.0019 0.0043 0.0057
Vacuole 0.0146 0.0044 0.0056 0.0155 0.0101 0.0044 0.0064 0.0033 0.0046 0.0126 0.0069 0.0046 0.0042 0.0029 0.0042 0.0126 0.0085 0.002
Vacuole Periphery 0.003 0.0007 0.001 0.0023 0.0024 0.0016 0.0011 0.0005 0.0012 0.0031 0.0012 0.0013 0.001 0.0003 0.0012 0.0029 0.0038 0.001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 44.7732 39.2187 33.8858 42.0336 32.3368 46.2501 43.049 49.3113 48.1311 48.0866
Translational Efficiency 1.2782 1.1894 1.3279 0.9539 1.4826 1.4008 1.1346 0.8985 1.078 1.1394

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency