Standard name
Human Ortholog
Description Multifunctional lipase/hydrolase/phospholipase; triacylglycerol lipase, steryl ester hydrolase, and Ca2+-independent phospholipase A2; catalyzes acyl-CoA dependent acylation of LPA to PA; required with Tgl3p for timely bud formation; phosphorylated and activated by Cdc28p; TGL4 has a paralog, TGL5, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.06 0 0 0 0.05 0 0.07 0.06 0 0 0 0 0 0 0.08 0.08 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.27 0.27 0.4 0.36 0.23 0.25 0.16 0.25 0.09 0.13 0.17 0.29 0.48 0.4 0.42 0.43 0.4 0.43 0.44 0.53 0.32 0.28 0.38
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.34 0.4 0.16 0.08 0.08 0.19 0.32 0.32 0.63 0.65 0.54 0.16 0.07 0.09 0.09 0.07 0.07 0 0.05 0 0 0.05 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0.08 0.05 0 0 0 0 0 0.08 0.11 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.29 0.16 0.1 0.17 0.13 0.2 0.2 0.22 0.25 0.3 0.26 0.19 0.22 0.3 0.29 0.23 0.25 0 0 0 0 0 0
Peroxisomes 0.23 0.38 0.2 0.33 0.16 0.18 0.07 0.05 0.06 0 0 0.61 0.58 0.52 0.28 0.26 0.27 0.21 0.26 0.24 0.45 0.48 0.41
SpindlePole 0.35 0.23 0.5 0.54 0.64 0.59 0.51 0.46 0.24 0.2 0.25 0.33 0.47 0.34 0.43 0.52 0.46 0.21 0.14 0.14 0.16 0.11 0.1
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 4 2 2 0 1 3 3 0 0 2 2 1 0 1 1 1 1 11 0 0 2 3
Bud 2 4 9 3 7 5 12 10 6 8 4 3 0 1 0 0 0 4 4 1 0 3 1
Bud Neck 1 1 3 1 3 1 0 1 1 0 0 0 0 0 0 1 1 0 0 0 0 0 1
Bud Site 3 2 3 0 2 3 2 1 1 3 1 1 1 0 0 0 0
Cell Periphery 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 19 4 10 7 16 11 23 20 8 3 5 12 10 13 26 36 30 1 0 2 0 0 0
Endoplasmic Reticulum 1 1 0 1 1 0 1 2 0 0 0 0 1 2 2 7 3 5 0 2 0 1 2
Endosome 91 157 111 74 74 64 53 85 27 38 46 105 220 148 130 183 106 158 294 177 33 92 103
Golgi 0 0 2 0 1 0 0 0 0 0 0 0 1 0 3 9 0 9 22 5 0 8 6
Mitochondria 114 233 45 16 26 49 106 108 185 189 148 58 32 35 28 30 18 11 35 5 3 15 6
Nucleus 2 2 0 0 1 5 4 7 5 11 5 1 3 3 1 1 0 2 0 0 0 0 0
Nuclear Periphery 7 47 13 2 3 5 8 15 23 31 31 3 2 6 4 6 3 2 9 1 0 0 0
Nucleolus 97 91 27 35 41 53 64 76 73 87 71 69 101 112 90 97 67 3 10 1 0 3 0
Peroxisomes 76 220 57 68 50 47 22 18 18 6 7 218 266 194 88 113 72 79 176 81 46 158 113
SpindlePole 117 133 141 111 205 153 166 158 71 57 68 119 214 127 133 224 122 77 93 46 17 35 26
Vac/Vac Membrane 7 8 2 4 3 3 5 1 3 3 8 0 6 2 12 17 15 3 2 1 0 0 0
Unique Cell Count 334 579 281 205 318 261 327 341 292 290 276 360 458 371 309 427 267 371 676 334 105 332 273
Labelled Cell Count 538 908 425 324 433 400 469 505 421 436 396 591 858 643 518 726 438 371 676 334 105 332 273


Lipid Particles

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.0 7.7 6.6 6.2 6.8 5.2 5.7 5.8 4.8 4.7 5.2 7.2 7.5 7.5 9.4 9.8 9.9 6.5 6.8 7.6
Std Deviation (1e-4) 1.3 1.4 1.2 1.2 1.5 1.5 1.8 1.5 1.0 1.2 1.1 1.8 1.4 1.5 1.8 2.1 1.9 1.6 1.9 1.5
Intensity Change (Log2) -0.1 0.04 -0.34 -0.22 -0.19 -0.47 -0.51 -0.35 0.11 0.18 0.17 0.51 0.56 0.58 -0.02 0.04 0.19


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud -1.2 -0.8 -0.9 0.3 -0.2 0 0 -1.4 -2.2 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.1 0.9 0.4 1.9 1.3 -0.6 -2.0 -1.3 -0.2 -1.1 0 2.5 2.6 3.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -0.8 -4.3 -3.7 -6.5 -3.9 0 0 -6.0 -2.7 2.3 0.1 0.6 0.9 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -2.7 -3.0 0.8 4.7 4.5 0 0 9.3 0 -3.9 -2.5 -2.6 -3.8 -3.4
Nucleus 0 0 0 0 0 0 3.3 0 0 0 0 0 0 0
Nuclear Periphery -2.3 -2.8 -1.8 -1.5 -0.1 0 0 2.9 -3.1 -3.9 -2.3 -2.4 -2.6 -2.4
Nucleolus 2.4 1.3 3.5 3.4 4.2 0 0 5.0 3.4 4.3 6.4 5.9 4.5 4.8
Peroxisomes 3.2 -1.5 -0.7 -5.0 -5.7 0 0 -6.6 10.2 10.1 8.3 2.3 1.9 1.8
SpindlePole 0.9 3.5 2.0 0.1 -1.0 0 0 -6.2 -4.4 -0.9 -4.1 -1.7 0.6 -1.1
Vacuole 0 0 0 0 0 0 0 0 0 0 0 2.5 2.6 3.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.4668 4.8158 3.9399 3.7713 4.0164 4.2637 1.9579 3.1331 2.5915 2.0174 2.0 2.4297 2.652 3.6059 3.6168 3.5742 2.8594 3.3856
Actin 0.0391 0.0035 0.0177 0.0001 0.0368 0.0047 0.0483 0.0001 0.0081 0 0.0011 0.0004 0.0009 0.001 0.0009 0.0002 0.0013 0.0004
Bud 0.0001 0.0001 0.0001 0 0.0001 0.0002 0.0003 0 0.0001 0 0.0001 0 0.0006 0.0003 0.0004 0 0.0018 0.0002
Bud Neck 0.0023 0.0006 0.0018 0.0001 0.0001 0.0003 0.0009 0.0001 0.0004 0 0.0001 0.0001 0.0007 0.0004 0.0011 0.0002 0.0011 0.0006
Bud Periphery 0.0002 0.0003 0.0002 0 0.0001 0.0005 0.0003 0.0001 0.0001 0 0.0001 0.0001 0.0005 0.0002 0.0005 0 0.0023 0.0002
Bud Site 0.0017 0.0083 0.0083 0.0001 0.0002 0.0002 0.0068 0.0002 0.0006 0 0.0002 0.0001 0.0016 0.0042 0.0084 0.0001 0.0092 0.0004
Cell Periphery 0.0001 0.0006 0.0003 0 0 0.0001 0.0001 0.0001 0.0001 0 0.0001 0 0.0001 0.0001 0.0001 0 0.0005 0.0001
Cytoplasm 0.0017 0.0001 0.0004 0.0002 0.001 0.0002 0.0023 0.0001 0.0001 0 0.0001 0 0.0014 0.0011 0.0004 0.0005 0.0037 0.0014
Cytoplasmic Foci 0.1913 0.1755 0.1737 0.1235 0.2295 0.1462 0.1832 0.1856 0.1771 0.1759 0.1762 0.13 0.1799 0.1843 0.1831 0.2038 0.1316 0.1497
Eisosomes 0.0003 0.0005 0.0007 0 0.0002 0 0.0002 0 0 0 0 0 0.0001 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0007 0.0001 0.0003 0 0.0005 0.0005 0.0014 0.0002 0.0001 0 0.0002 0 0.0008 0.0003 0.0001 0.0002 0.0009 0.0002
Endosome 0.0095 0.0047 0.0044 0.0483 0.0107 0.0067 0.0529 0.0215 0.017 0.0087 0.0218 0.0058 0.0325 0.0355 0.019 0.0158 0.0307 0.0104
Golgi 0.0116 0.0085 0.0087 0.012 0.0124 0.0108 0.0201 0.0163 0.0209 0.012 0.0168 0.0109 0.0201 0.022 0.0158 0.0189 0.017 0.013
Lipid Particles 0.6206 0.6568 0.6608 0.7026 0.5822 0.752 0.5767 0.6613 0.6879 0.7086 0.6042 0.7912 0.5834 0.5901 0.5902 0.612 0.585 0.6701
Mitochondria 0.0016 0.004 0.0012 0.0014 0.0012 0.0031 0.0028 0.0009 0.0011 0.0003 0.0161 0.004 0.0102 0.003 0.008 0.0019 0.0248 0.0015
None 0.0071 0.0002 0.004 0 0.0084 0.0001 0.0011 0.0001 0 0 0.0001 0 0.0028 0.0005 0.0008 0.0002 0.0138 0.0014
Nuclear Periphery 0.0009 0.0002 0.0007 0.0001 0.0035 0.0036 0.0052 0.0054 0.0001 0 0.0005 0.0004 0.0059 0.006 0.0017 0.0131 0.0042 0.0031
Nucleolus 0.0035 0.0161 0.0048 0.0002 0.0003 0.0015 0.0041 0.013 0.0081 0.0002 0.0008 0.0026 0.0162 0.0246 0.014 0.0011 0.0113 0.01
Nucleus 0.0006 0.0005 0.0016 0 0.0003 0.0065 0.0012 0.0005 0.0004 0 0.0002 0.0001 0.0078 0.0029 0.0022 0.0009 0.0135 0.0036
Peroxisomes 0.0848 0.066 0.0836 0.1024 0.0976 0.0424 0.0663 0.0542 0.0512 0.0908 0.1123 0.0306 0.0765 0.0692 0.0644 0.0791 0.0576 0.0679
Punctate Nuclear 0.022 0.0528 0.026 0.0077 0.0142 0.0183 0.0189 0.0364 0.0256 0.0031 0.0445 0.0233 0.0548 0.0478 0.0867 0.0511 0.0689 0.0646
Vacuole 0.0003 0.0004 0.0003 0.0009 0.0001 0.0011 0.0049 0.0024 0.0007 0.0001 0.0019 0.0002 0.0019 0.0044 0.0012 0.0004 0.0175 0.0007
Vacuole Periphery 0.0003 0.0002 0.0002 0.0003 0.0003 0.0009 0.002 0.0016 0.0003 0.0002 0.0027 0.0002 0.0011 0.0021 0.0007 0.0005 0.0033 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 17.5624 18.4066 23.0319 32.8261 24.0791 24.6141 24.9057 32.511 32.6554 28.0112
Translational Efficiency 0.5727 0.5298 0.4467 0.5268 0.4365 0.4962 0.5067 0.4159 0.502 0.4407

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1978 1356 2160 1376 1874 1516 265 904 3852 2872 2425 2280

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 775.69 847.10 1097.35 1009.54 794.68 852.49 1026.98 1026.86 784.93 849.95 1089.66 1016.41
Standard Deviation 103.13 138.34 145.81 171.12 107.62 125.04 128.90 204.88 105.76 131.51 145.72 185.44
Intensity Change Log 2 0.127052 0.500472 0.380146 0.101309 0.369962 0.369794 0.114082 0.435903 0.374916

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000847 0.002211 0.008563 0.057057 0.000463 0.001713 0.010341 0.032805 0.000660 0.001948 0.008758 0.047442
Bud Neck 0.000417 0.001268 0.004675 0.003231 0.000612 0.001252 0.005577 0.004231 0.000512 0.001260 0.004774 0.003628
Bud Site 0.000319 0.000471 0.004557 0.004693 0.000205 0.000626 0.003244 0.003067 0.000264 0.000553 0.004413 0.004048
Cell Periphery 0.000042 0.000055 0.000080 0.000135 0.000029 0.000055 0.000096 0.000133 0.000036 0.000055 0.000082 0.000134
Cytoplasm 0.000421 0.000556 0.002157 0.001978 0.000312 0.000489 0.001002 0.001876 0.000368 0.000520 0.002031 0.001937
Cytoplasmic Foci 0.042223 0.097969 0.145888 0.236930* 0.060182 0.088203 0.171129 0.207237* 0.050960 0.092814 0.148646 0.225157*
Eisosomes 0.000144 0.000195 0.000049 0.000246 0.000108 0.000125 0.000120 0.000242 0.000126 0.000158 0.000057 0.000244
Endoplasmic Reticulum 0.000385 0.000650 0.001323 0.001057 0.000347 0.000417 0.000871 0.000709 0.000366 0.000527 0.001274 0.000919
Endosome 0.009990 0.028947 0.078148 0.103614 0.013092 0.042540 0.081406 0.089392 0.011499 0.036122 0.078504 0.097975
Golgi 0.024629 0.050587 0.008360 0.131926* 0.022895 0.036308 0.031974 0.143572* 0.023785 0.043050 0.010940 0.136544*
Lipid Particles 0.863010* 0.731339* 0.386332* 0.270944* 0.831559* 0.718455* 0.427875* 0.374775* 0.847709* 0.724538* 0.390872* 0.312112*
Mitochondria 0.002514 0.002266 0.001300 0.029514 0.001254 0.002581 0.002560 0.020324 0.001901 0.002432 0.001437 0.025870
Mitotic Spindle 0.009606 0.017416 0.224964* 0.039475 0.013949 0.013225 0.140128* 0.035701 0.011719 0.015204 0.215693* 0.037978
None 0.000403 0.000077 0.000034 0.000107 0.000086 0.000068 0.000035 0.000412 0.000249 0.000072 0.000034 0.000228
Nuclear Periphery 0.000532 0.000862 0.003406 0.001694 0.000490 0.000613 0.003498 0.003155 0.000511 0.000730 0.003416 0.002273
Nuclear Periphery Foci 0.017435 0.021465 0.062543 0.003648 0.020742 0.027279 0.050368 0.008158 0.019044 0.024534 0.061213 0.005436
Nucleolus 0.001239 0.001431 0.002497 0.000131 0.001182 0.001280 0.000478 0.000575 0.001211 0.001351 0.002276 0.000307
Nucleus 0.000630 0.000354 0.001030 0.000197 0.000196 0.000455 0.000269 0.000186 0.000419 0.000407 0.000947 0.000193
Peroxisomes 0.024759 0.039978 0.055777 0.098018 0.031611 0.062919 0.060335 0.053718 0.028092 0.052088 0.056275 0.080454
Vacuole 0.000363 0.001605 0.007849 0.011548 0.000600 0.001206 0.008013 0.014039 0.000478 0.001395 0.007867 0.012536
Vacuole Periphery 0.000095 0.000298 0.000469 0.003856 0.000086 0.000192 0.000682 0.005694 0.000091 0.000242 0.000493 0.004585

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.89 -6.77 -14.99 -14.53 -12.44 -1.90 -4.60 -9.28 -8.78 -5.53 -3.12 -7.81 -17.57 -16.90 -13.63
Bud Neck -4.65 -12.39 -8.38 -5.38 3.80 -2.79 -5.36 -7.71 -5.99 1.28 -5.07 -13.25 -11.30 -7.97 3.49
Bud Site -1.18 -9.39 -6.92 -6.70 0.81 -2.42 -5.46 -8.33 -6.58 0.15 -2.57 -10.20 -9.42 -8.58 1.67
Cell Periphery -1.35 -4.45 -8.66 -10.59 -6.29 -3.05 -7.19 -8.24 -5.40 -2.55 -2.89 -8.22 -12.76 -10.26 -6.34
Cytoplasm -1.35 -4.18 -11.80 -14.59 1.41 -3.90 -4.60 -3.93 -3.54 -2.09 -2.69 -4.45 -8.64 -8.07 1.10
Cytoplasmic Foci -17.32 -27.22 -34.75 -22.67 -14.43 -8.75 -11.04 -23.42 -18.25 -3.26 -18.49 -27.68 -41.28 -29.51 -15.02
Eisosomes -2.78 8.96 -6.33 -2.66 -16.50 -1.50 -0.39 -7.51 -6.65 -4.39 -2.96 9.85 -10.05 -6.90 -18.80
Endoplasmic Reticulum -3.75 -3.46 -5.69 -3.17 1.38 -0.97 -4.96 -5.88 -4.63 1.63 -3.41 -3.70 -7.31 -4.97 1.82
Endosome -8.49 -24.07* -26.18* -18.57 -6.04 -10.45 -8.57 -17.77 -9.48 -0.90 -13.49 -25.51 -31.43 -19.55 -5.51
Golgi -11.25 15.00 -24.67 -17.23 -29.07* -7.62 -2.28 -21.14 -18.46 -16.64 -13.40 14.23 -32.56 -25.84 -36.54*
Lipid Particles 15.56 56.91* 66.67* 43.98* 10.01 12.39 19.30 38.21* 26.64 2.18 19.79 60.75 73.92 50.63* 7.46
Mitochondria 0.42 2.14 -9.41 -9.60 -9.96 -1.94 -1.09 -7.11 -6.60 -6.22 -1.14 1.09 -11.80 -11.58 -12.12
Mitotic Spindle -2.88 -31.00* -8.05 -5.41 24.59* 0.60 -8.05 -4.64 -4.99 6.51 -1.67 -31.76 -8.97 -7.63 26.10*
None 1.16 1.29 1.06 -2.16 -6.07 0.67 1.73 -1.09 -1.19 -1.30 1.24 1.46 0.13 -1.34 -1.62
Nuclear Periphery -3.40 -12.15 -3.96 -2.79 4.76 -2.02 -3.62 -5.51 -5.24 0.35 -3.88 -12.83 -6.75 -5.87 3.46
Nuclear Periphery Foci -1.50 -12.18 6.84 7.62 17.75 -1.95 -3.22 5.24 6.71 4.69 -2.53 -12.68 8.87 10.48 17.85
Nucleolus -0.55 -1.94 3.19 3.61 4.08 -0.08 1.68 1.33 1.29 -0.39 -0.39 -1.89 3.23 3.32 3.78
Nucleus 1.06 -1.94 1.65 1.23 3.84 -1.16 -0.65 -0.02 1.16 0.64 0.07 -2.92 1.52 1.56 4.03
Peroxisomes -4.17 -8.89 -13.53 -9.78 -7.46 -7.07 -3.30 -4.32 1.61 0.67 -8.28 -8.93 -13.50 -6.57 -5.64
Vacuole -7.57 -10.55 -8.17 -7.21 -1.32 -4.20 -4.31 -8.68 -8.28 -2.25 -8.51 -11.18 -11.70 -10.77 -2.47
Vacuole Periphery -5.63 -6.03 -6.65 -6.28 -5.93 -3.28 -4.36 -5.49 -5.39 -4.86 -6.30 -7.02 -8.49 -8.19 -7.66