Standard name
Description Protein that localizes to sites of polarized growth; required for selection and/or maintenance of polarized growth sites, may modulate signaling by the GTPases Cdc42p and Rho1p; contains LIM domains and has similarity to metazoan paxillin; relocalizes from bud neck to cytoplasm upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.2 0.14 0.18 0.1 0.11 0.08 0.05 0 0.06 0.05 0 0 0.09 0 0 0.18 0.16 0.17 0.09 0 0 0.07 0.14 0.08
Bud 0.23 0.1 0.12 0 0.07 0.07 0.08 0.06 0.08 0.09 0.07 0.07 0.06 0 0 0.06 0.07 0.17 0.3 0.22 0.19 0.13 0.07 0.11
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.18 0.16 0.17 0.16 0.06 0.06
Bud Site 0.12 0.08 0.13 0.09 0 0.06 0 0 0 0 0 0 0.1 0 0 0.1 0.07 0.06 – – – – – –
Cell Periphery 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.15 0.13 0.17 0 0 0 0 0 0
Cytoplasm 0.48 0.66 0.64 0.76 0.8 0.71 0.76 0.77 0.64 0.64 0.67 0.69 0.78 0.95 0.98 0.61 0.62 0.5 0.33 0.48 0.46 0.43 0.56 0.59
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0
Endosome 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.14 0.15 0.12 0.39 0.34 0.37 0.25 0 0 0 0.06 0 0.22 0 0 0.05 0.05 0 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0.06 0 0 0 0 0 0
SpindlePole 0.11 0 0.14 0.1 0.05 0.1 0.07 0.06 0 0 0 0 0 0 0 0.08 0.07 0.06 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 65 38 60 29 36 35 27 15 19 17 6 9 10 6 1 16 16 3 32 12 15 3 20 13
Bud 74 27 39 13 25 32 46 32 27 29 24 31 7 3 2 5 7 3 107 66 70 6 10 18
Bud Neck 7 9 13 6 12 13 16 15 1 5 3 11 0 0 0 0 2 0 64 46 62 8 8 9
Bud Site 38 21 44 26 12 28 14 6 10 7 3 4 11 2 1 9 7 1 – – – – – –
Cell Periphery 9 10 9 16 3 2 6 7 9 2 5 7 1 8 1 13 13 3 2 1 2 0 2 1
Cytoplasm 153 174 212 222 272 317 430 431 216 212 219 288 87 190 228 53 63 9 116 142 169 23 80 94
Endoplasmic Reticulum 2 8 6 2 3 3 2 0 2 5 2 4 1 0 0 2 3 0 2 1 1 1 8 5
Endosome 22 7 1 1 1 3 3 4 0 0 0 0 0 0 1 2 0 1 5 4 2 1 1 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 2 0 0 0
Mitochondria 14 11 2 6 7 62 83 66 130 114 121 104 3 1 4 5 3 4 3 3 17 2 5 9
Nucleus 6 0 3 2 0 3 3 9 3 2 2 4 0 2 0 1 3 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 2 1 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 3 3 1 1 0 4 4 7 1 1 1 2 2 0 0 1 1 0 0 0 0 0 0 0
Peroxisomes 19 12 2 4 0 15 4 1 3 2 0 1 7 3 2 0 2 1 0 0 1 1 0 0
SpindlePole 35 5 45 30 18 47 38 34 12 9 7 17 2 1 0 7 7 1 8 5 8 0 0 1
Vac/Vac Membrane 4 3 6 4 3 4 3 23 2 8 5 2 0 0 0 3 10 0 4 8 7 1 4 3
Unique Cell Count 320 264 332 292 340 448 568 560 336 332 327 418 112 199 233 87 101 18 353 297 368 54 143 160
Labelled Cell Count 451 328 443 362 392 568 679 652 436 415 398 484 131 216 240 117 138 26 353 297 368 54 143 160


Ambiguous, Bud, Bud Neck, Cell Periphery, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.3 6.4 6.3 5.8 6.3 4.9 4.9 5.1 4.2 4.0 4.0 4.4 6.0 6.4 6.4 10.0 10.5 9.9 5.8 5.8 6.3
Std Deviation (1e-4) 0.9 1.1 0.7 1.0 1.3 1.0 0.9 1.0 0.8 0.6 0.7 0.8 0.6 0.9 1.1 1.7 2.0 1.7 1.6 1.4 1.5
Intensity Change (Log2) – – – -0.12 -0.01 -0.36 -0.38 -0.3 -0.58 -0.65 -0.67 -0.51 -0.08 0.02 0.03 0.67 0.74 0.65 -0.12 -0.12 0.0


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -2.9 -2.8 -4.3 0 -8.0 0 0 0 0 -2.3 -5.1 -6.7 0.1 -0.5 0
Bud -3.3 -1.9 -2.2 0 -3.2 0 0 0 0 -1.7 -4.2 -4.9 -1.6 -1.4 0
Bud Neck -1.3 -0.3 -0.8 0 -1.0 0 0 0 0 0 0 -3.1 0 0 0
Bud Site -1.7 -4.6 -3.3 0 -7.6 0 0 0 0 -1.0 -4.9 -5.5 -0.7 -1.7 0
Cell Periphery 1.8 -1.8 0 0 -1.6 0 0 0 0 0 0.8 0 0 4.1 0
Cytoplasm 3.3 4.7 2.0 3.8 4.2 0.1 0 0.8 1.5 2.7 8.2 9.6 -0.5 -0.3 -1.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 6.7 0 6.1 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 2.6 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole -1.3 -3.7 -1.3 0 -3.8 0 0 0 0 -3.5 -5.2 -5.9 -1.4 -1.8 0
Vacuole 0 0 0 0 1.9 0 0.5 -0.3 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.5217 1.5338 1.7657 1.857 1.7957 1.5617 1.5609 1.8027 2.0236 1.8129 1.6663 1.6905 1.3612 1.657 1.4639 2.2408 1.3683 1.5724
Actin 0.039 0.0268 0.0202 0.0123 0.004 0.0266 0.0413 0.0106 0.0288 0.0313 0.0007 0.0225 0.1187 0.0065 0.0378 0.002 0.0152 0.0107
Bud 0.0551 0.0531 0.2451 0.0274 0.0111 0.0072 0.0485 0.0463 0.168 0.0702 0.0015 0.0079 0.0539 0.0622 0.2089 0.0315 0.0156 0.0055
Bud Neck 0.0803 0.0062 0.0057 0.0455 0.0415 0.5547 0.0851 0.0048 0.0099 0.0036 0.0526 0.5746 0.0571 0.0058 0.0125 0.0135 0.0242 0.4855
Bud Periphery 0.0908 0.0367 0.3008 0.0812 0.011 0.01 0.0403 0.0358 0.2133 0.0651 0.0017 0.0106 0.0741 0.0447 0.2645 0.0713 0.0346 0.0054
Bud Site 0.1936 0.3048 0.0812 0.065 0.0284 0.0071 0.2204 0.389 0.0927 0.0502 0.0023 0.0233 0.1439 0.2542 0.0343 0.0354 0.0616 0.0131
Cell Periphery 0.003 0.002 0.0019 0.0036 0.0004 0.0009 0.0058 0.0026 0.0027 0.0016 0.0004 0.0008 0.0036 0.0023 0.0013 0.0009 0.0022 0.0005
Cytoplasm 0.2716 0.4446 0.243 0.5295 0.7275 0.2466 0.297 0.39 0.3497 0.5567 0.77 0.2889 0.3822 0.4432 0.3252 0.69 0.5504 0.3317
Cytoplasmic Foci 0.0777 0.03 0.0208 0.0321 0.0674 0.0594 0.0819 0.038 0.0232 0.0388 0.0387 0.0304 0.0335 0.0208 0.022 0.0056 0.0301 0.0829
Eisosomes 0.0004 0.0002 0.0002 0.0003 0 0.0001 0.0003 0.0001 0.0002 0.0004 0 0 0.0008 0.0001 0.0003 0.0001 0.0004 0
Endoplasmic Reticulum 0.0045 0.0023 0.0024 0.0045 0.0044 0.0019 0.0099 0.0024 0.0042 0.0035 0.0025 0.0011 0.0051 0.0019 0.0036 0.0031 0.007 0.0006
Endosome 0.0407 0.0071 0.0112 0.0382 0.0409 0.013 0.0295 0.0085 0.026 0.028 0.0586 0.0077 0.0191 0.0025 0.0232 0.0041 0.0744 0.0076
Golgi 0.0128 0.0035 0.003 0.0104 0.0041 0.011 0.011 0.002 0.0117 0.0076 0.0177 0.0037 0.0087 0.0008 0.0063 0.0003 0.0085 0.0019
Lipid Particles 0.0151 0.001 0.004 0.0026 0.0198 0.0023 0.0144 0.0003 0.0049 0.0051 0.0108 0.0005 0.0054 0.0003 0.0026 0.0002 0.0109 0.0021
Mitochondria 0.0242 0.006 0.008 0.0054 0.0059 0.0115 0.0291 0.0004 0.0038 0.03 0.0019 0.0055 0.004 0.0005 0.0125 0.0003 0.009 0.0005
None 0.0345 0.056 0.0348 0.099 0.0064 0.033 0.0309 0.0557 0.0465 0.0749 0.0065 0.0136 0.0619 0.1379 0.0319 0.1359 0.0545 0.0394
Nuclear Periphery 0.0114 0.0007 0.0012 0.0036 0.0014 0.0006 0.0184 0.0006 0.0013 0.0014 0.001 0.0004 0.0042 0.0006 0.0022 0.0006 0.0092 0.0001
Nucleolus 0.0038 0.0005 0.0004 0.0007 0.0012 0.0003 0.0006 0.0004 0.0004 0.0007 0.0004 0.0002 0.0019 0.0011 0.0003 0.0001 0.0124 0.0002
Nucleus 0.0095 0.002 0.0013 0.021 0.0016 0.0007 0.0061 0.0018 0.0016 0.0023 0.0012 0.0011 0.0022 0.0067 0.0009 0.0008 0.021 0.0004
Peroxisomes 0.0165 0.007 0.0075 0.0017 0.0094 0.008 0.0131 0.0034 0.0044 0.0105 0.0078 0.0037 0.0063 0.001 0.0032 0.0001 0.0094 0.0093
Punctate Nuclear 0.0075 0.001 0.002 0.0023 0.0063 0.0028 0.003 0.0025 0.0016 0.0019 0.0074 0.0012 0.0086 0.0016 0.0012 0.0004 0.027 0.0013
Vacuole 0.0058 0.0074 0.0044 0.0112 0.0059 0.0016 0.0115 0.0045 0.004 0.0144 0.013 0.002 0.004 0.005 0.0042 0.0035 0.0174 0.001
Vacuole Periphery 0.002 0.0009 0.0008 0.0025 0.0014 0.0004 0.002 0.0003 0.001 0.0017 0.0032 0.0004 0.001 0.0003 0.001 0.0005 0.0049 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 22.6299 8.836 10.0076 18.6484 24.6192 15.0783 15.9873 11.9011 11.8447 23.1497
Translational Efficiency 1.5384 2.4336 1.4636 1.0212 1.2668 2.2851 1.007 1.4047 1.5123 1.3192

Hit Data

Dataset Hit
Protein Abundance ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘