Standard name
Description High-affinity cysteine-specific transporter; has similarity to the Dal5p family of transporters; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YCT1 is not an essential gene

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.94 0.97 0.96 0.92 0.87 0.85 0.73 0.76 0.67 0.71 0.99 0.99 1.0 0.77 0.77 0.75 0.82 0.79 0.52 0.73 0.76 0.78
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.11 0 0 0.05 0.21 0.13 0.33 0.34 0.4 0.35 0 0 0 0.05 0 0 0.12 0 0.21 0.13 0.1 0.07
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0.22 0.3 0.33 0 0.09 0.08 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 3 3 0 0 0 0 0 0 17 9 11 12
Bud 0 0 2 3 7 16 31 15 30 38 3 3 0 0 0 1 0 0 11 3 10 17
Bud Neck 0 3 2 2 6 7 5 8 7 5 46 50 25 0 0 0 0 0 9 3 5 11
Bud Site 0 0 0 0 0 1 1 3 1 5 0 0 0 0 0 0
Cell Periphery 1 5 4 1 4 8 7 2 6 8 17 17 6 0 0 0 0 0 6 12 12 21
Cytoplasm 44 606 492 678 691 915 658 868 519 688 1317 1317 1323 68 183 117 19 51 317 364 456 635
Endoplasmic Reticulum 0 3 2 0 0 0 2 2 5 0 1 0 1 3 9 11 0 0 16 7 3 2
Endosome 0 4 5 24 8 21 11 13 11 7 1 1 0 0 3 3 0 0 20 1 2 3
Golgi 0 2 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 5 0 0 0
Mitochondria 5 5 12 36 171 143 292 383 312 339 0 0 0 4 4 4 2 2 130 65 59 59
Nucleus 0 1 0 1 1 2 2 2 5 8 2 2 2 11 8 3 0 0 1 0 0 1
Nuclear Periphery 0 0 0 0 1 2 1 2 3 6 0 0 0 0 1 0 0 0 0 0 1 0
Nucleolus 0 2 1 0 0 3 2 0 2 1 0 0 0 0 1 0 0 0 0 0 0 1
Peroxisomes 0 1 6 9 4 6 0 2 5 2 0 0 0 0 0 0 0 0 1 0 0 1
SpindlePole 0 5 3 18 12 39 45 46 26 30 3 3 1 0 0 0 0 0 7 7 10 15
Vac/Vac Membrane 0 4 4 12 15 20 17 28 15 31 2 3 0 19 72 51 0 5 48 9 10 8
Unique Cell Count 47 627 510 739 797 1081 898 1136 772 969 1328 1328 1324 88 238 155 24 65 613 502 602 817
Labelled Cell Count 50 641 533 784 920 1183 1074 1374 947 1168 1395 1399 1358 105 282 190 24 65 613 502 602 817


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.0 4.0 4.1 3.9 3.1 3.5 3.2 2.9 3.3 3.1 5.8 5.8 5.9 5.1 6.0 6.8 5.1 5.3 5.3
Std Deviation (1e-4) 0.4 1.4 1.9 1.7 1.1 1.3 1.2 0.9 1.2 1.0 0.9 0.9 0.9 1.2 1.5 1.8 1.3 1.2 1.4
Intensity Change (Log2) 0.06 -0.01 -0.34 -0.17 -0.31 -0.44 -0.27 -0.38 0.55 0.55 0.58 0.35 0.59 0.77 0.36 0.41 0.42


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0
Bud Neck 0 0
Bud Site 0 0
Cell Periphery 0 0
Cyto
Endoplasmic Reticulum 0 0
Endosome 0 0
Golgi 0 0
Mitochondria 0 0
Nuclear Periphery 0 0
Nuc
Nucleolus 0 0
Peroxisomes 0 0
SpindlePole 0 0
Vac
Cortical Patches 0 0
Cytoplasm 0 0
Nucleus 0 0
Vacuole 13.7 14.1