Standard name
Human Ortholog
Description Protein involved in prospore membrane morphogenesis; peripheral membrane protein that localizes to the prospore membrane and at numerous membrane contact sites; involved in sporulation, vacuolar protein sorting, prospore membrane formation during sporulation, and protein-Golgi retention; required for mitochondrial integrity; contains a PH-like domain; homologous to human CHAC and COH1 which are involved in Chorea-acanthocytosis and Cohen syndrome, respectively

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.09 0.09 0.06 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Bud 0 0 0.09 0 0 0 0 0.08 0.05 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.72 0.88 0.57 0.35 0.38 0.4 0.33 0.34 0.37 0.32 0.44 0.7 0.61 0.62 0.76 0.59 0.73 0.49 0.48 0.27 0.38 0.29 0.29
Endoplasmic Reticulum 0.18 0.06 0.14 0.12 0.08 0.14 0.06 0 0 0 0 0.14 0.16 0.13 0.07 0.06 0 0 0 0 0 0.08 0.07
Endosome 0.1 0.06 0.05 0.34 0.39 0.14 0.13 0.09 0.06 0 0 0.13 0.29 0.24 0.11 0.19 0.09 0.28 0.24 0.37 0.31 0.39 0.35
Golgi 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0
Mitochondria 0 0 0.17 0.25 0.06 0.36 0.43 0.61 0.57 0.75 0.63 0 0 0 0 0 0 0 0 0.11 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.11 0.09 0.13 0.1 0.07 0.08 0.07 0.06 0 0 0.12 0.17 0.13 0.11 0.16 0.13 0.18 0.18 0.2
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 8 0 7 20 21 6 15 2 8 2 2 6 5 7 4 9 8 2 0 1 0 0 1
Bud 1 0 7 7 9 8 18 35 25 22 21 3 2 0 0 0 0 0 0 0 0 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 2 0 1 1
Bud Site 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cell Periphery 2 0 0 0 1 1 0 5 2 2 5 1 0 2 1 4 4 0 0 0 0 0 0
Cytoplasm 159 14 43 80 145 136 192 156 193 107 191 109 123 152 100 93 140 120 28 34 21 47 62
Endoplasmic Reticulum 39 1 11 27 31 47 33 17 21 8 15 22 32 31 9 10 8 10 2 2 1 14 15
Endosome 21 1 4 79 148 47 78 39 33 5 9 20 59 59 14 30 18 70 14 46 17 64 75
Golgi 3 1 1 5 7 6 2 0 1 0 0 0 0 1 0 12 8 4 0 0 0 1 4
Mitochondria 3 0 13 57 24 120 255 279 299 252 271 1 0 2 1 4 4 4 1 14 1 3 2
Nucleus 1 0 0 0 1 0 3 0 1 3 3 0 0 2 0 1 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 2 0 2 7 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Nucleolus 0 0 0 1 0 2 15 3 4 1 0 0 0 0 0 3 3 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 2 0 4 4 5 3 11 8 11 3 5 3 1 0 1 4 0 2 0 2 0 1 2
Vac/Vac Membrane 9 0 1 1 43 31 77 46 35 28 29 10 6 10 16 27 26 26 9 16 10 30 41
Unique Cell Count 221 16 76 229 378 336 590 454 520 337 432 155 203 244 132 157 193 248 59 127 56 168 214
Labelled Cell Count 248 17 91 283 435 409 706 590 635 433 552 175 228 266 146 197 219 248 59 127 56 168 214


Cytoplasm, Endosome

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.7 12.1 5.4 6.6 7.6 6.2 5.8 5.3 5.1 4.8 4.6 8.9 9.0 9.2 12.8 12.7 12.2 8.0 9.1 9.4
Std Deviation (1e-4) 1.2 1.2 1.2 1.0 1.4 1.0 1.1 1.0 0.9 1.4 0.9 1.4 1.2 1.5 2.8 2.9 2.7 1.4 1.5 1.6
Intensity Change (Log2) 0.27 0.49 0.19 0.09 -0.04 -0.08 -0.18 -0.24 0.71 0.72 0.76 1.24 1.23 1.17 0.55 0.75 0.78


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.1 0 0 0 0 0 0 0 0 0 0 0 -1.0 0
Bud 0 0 0 0 -0.4 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -3.3 -2.9 -2.6 -4.1 -3.7 -3.2 -4.1 -2.0 2.1 0.6 0.9 2.9 0.4 2.5
Endoplasmic Reticulum -0.6 -1.7 -0.1 -2.9 0 0 0 0 -0.1 0.3 -0.4 -1.8 -2.0 -3.0
Endosome 5.0 5.7 2.1 2.0 1.0 0 0 0 1.8 4.2 3.6 1.3 2.8 1.1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.4 -3.1 3.1 4.4 7.2 6.6 0 0 0 0 0 -4.5 -4.0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 2.7 2.3 3.0 2.5 0 2.2 0 0 0 0 2.7 3.5 3.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.6589 6.9169 5.9089 5.0376 4.7929 5.9508 4.5355 6.6779 5.2796 4.7315 4.2206 5.3402 6.4948 8.2403 7.8774 6.868 7.3242 7.9352
Actin 0.0373 0.0007 0.0113 0.0017 0.0011 0.0044 0.0147 0.0002 0.0089 0.0012 0.0215 0.0007 0.0113 0.0001 0.0007 0.002 0.0036 0.0028
Bud 0.0019 0.0062 0.0031 0.0056 0.0003 0.0002 0.0008 0.0001 0.0005 0.0002 0.0008 0.0002 0.0006 0.0003 0.0003 0.0026 0.0005 0.0002
Bud Neck 0.0013 0.0003 0.0015 0.0004 0.0005 0.002 0.0014 0.0002 0.0009 0.0002 0.0007 0.0007 0.0008 0.0002 0.0003 0.0294 0.0003 0.0005
Bud Periphery 0.0025 0.0013 0.0009 0.0022 0.0002 0.0001 0.0008 0 0.0003 0.0001 0.0005 0.0001 0.0005 0.0001 0.0001 0.0049 0.0004 0.0001
Bud Site 0.0011 0.0115 0.0119 0.0008 0.0002 0.0002 0.0043 0.0004 0.0014 0.0002 0.0025 0.0003 0.0061 0.0019 0.0007 0.0242 0.0005 0.0001
Cell Periphery 0.0003 0.0002 0.0003 0.0001 0 0 0.0004 0.0001 0.0004 0 0.0001 0 0.0005 0.0001 0.0001 0.001 0.0001 0
Cytoplasm 0.2115 0.4841 0.3479 0.2487 0.2788 0.3059 0.2377 0.4758 0.3689 0.2736 0.2136 0.3329 0.3404 0.5442 0.493 0.2662 0.2313 0.344
Cytoplasmic Foci 0.0985 0.097 0.0936 0.085 0.1386 0.1257 0.0865 0.0914 0.0906 0.0982 0.1557 0.116 0.0983 0.1149 0.1226 0.0999 0.1245 0.1162
Eisosomes 0.0004 0 0.0001 0 0 0 0.0001 0 0.0001 0 0.0001 0 0.0002 0 0 0 0 0
Endoplasmic Reticulum 0.0235 0.0101 0.012 0.0075 0.0096 0.0169 0.0277 0.0072 0.0112 0.0044 0.0045 0.0125 0.036 0.0062 0.0072 0.0026 0.0125 0.0099
Endosome 0.5059 0.3422 0.4481 0.5433 0.4355 0.4828 0.5324 0.382 0.4304 0.525 0.5308 0.4709 0.3625 0.2869 0.3279 0.4659 0.4813 0.4651
Golgi 0.0324 0.0127 0.0216 0.0209 0.0363 0.0271 0.0302 0.0125 0.03 0.0137 0.029 0.035 0.024 0.0095 0.0177 0.0306 0.049 0.0432
Lipid Particles 0.0071 0.0013 0.0054 0.0122 0.002 0.0017 0.0126 0.001 0.0054 0.0008 0.0055 0.0013 0.0121 0.0007 0.0005 0.0088 0.0017 0.0005
Mitochondria 0.0049 0.0013 0.0027 0.0058 0.0314 0.0082 0.007 0.0005 0.0065 0.0339 0.0123 0.0038 0.0052 0.0005 0.0023 0.0119 0.0604 0.0038
None 0.0089 0.0016 0.0015 0.0028 0.0048 0.0015 0.0014 0.0014 0.0038 0.0004 0.0007 0.0007 0.0421 0.0012 0.0063 0.0006 0.0016 0.0004
Nuclear Periphery 0.0175 0.0016 0.0034 0.007 0.0173 0.0028 0.0072 0.0009 0.0018 0.0019 0.0008 0.0019 0.0067 0.0011 0.001 0.0011 0.0012 0.0009
Nucleolus 0.0002 0.0001 0.0002 0.0002 0.0001 0.0001 0.0002 0 0.0001 0 0.0001 0.0001 0.0007 0.0001 0 0.0022 0 0
Nucleus 0.008 0.0009 0.0014 0.0021 0.0022 0.0008 0.0011 0.0005 0.0006 0.0005 0.0004 0.0011 0.0072 0.0007 0.0006 0.0014 0.0005 0.0003
Peroxisomes 0.0052 0.0005 0.0033 0.0093 0.0126 0.0009 0.0029 0.0003 0.01 0.001 0.0047 0.0017 0.0025 0.0008 0.0006 0.0026 0.0075 0.0026
Punctate Nuclear 0.0016 0.0003 0.001 0.0018 0.0006 0.0004 0.0029 0.0002 0.0005 0.0002 0.0006 0.0004 0.0046 0.0002 0.0003 0.0004 0.0002 0.0001
Vacuole 0.023 0.0218 0.022 0.0301 0.0214 0.0132 0.0217 0.0211 0.0192 0.0329 0.0098 0.0125 0.0325 0.0263 0.0133 0.0322 0.0143 0.0057
Vacuole Periphery 0.0069 0.0045 0.0069 0.0125 0.0066 0.0051 0.0059 0.0041 0.0086 0.0115 0.0053 0.0071 0.0053 0.0041 0.0044 0.0095 0.0084 0.0036

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 33.0378 24.2543 31.8063 43.8604 29.7949 44.1819 38.6879 43.8049 44.9496 45.7611
Translational Efficiency 0.7227 0.6238 0.6942 0.5472 0.651 0.529 0.4956 0.3704 0.4961 0.5184

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
35 1921 173 1171 1521 1251 136 1236 1556 3172 309 2407

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 972.18 998.39 1312.84 1171.51 870.12 1012.68 1320.32 1153.95 872.42 1004.03 1316.13 1162.49
Standard Deviation 161.15 155.20 152.74 164.13 121.19 135.10 144.51 159.78 123.17 147.77 149.22 162.15
Intensity Change Log 2 0.038380 0.433396 0.269074 0.218892 0.601601 0.407294 0.126455 0.515287 0.336008

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000887 0.000575 0.003455 0.004156 0.001065 0.000269 0.003441 0.002115 0.001061 0.000454 0.003449 0.003108
Bud Neck 0.005443 0.002119 0.003152 0.004492 0.006293 0.004575 0.010528 0.004357 0.006274 0.003088 0.006399 0.004423
Bud Site 0.027236 0.026837 0.035434 0.063879 0.027256 0.025403 0.056254 0.059762 0.027256 0.026272 0.044598 0.061765
Cell Periphery 0.000678 0.000145 0.000215 0.000459 0.000320 0.000203 0.001786 0.000227 0.000328 0.000168 0.000907 0.000340
Cytoplasm 0.063450 0.065586 0.023571 0.142593* 0.070192 0.060626 0.039737 0.167938* 0.070040 0.063630 0.030686 0.155608*
Cytoplasmic Foci 0.333914* 0.320173* 0.240551* 0.229204* 0.306899* 0.291106* 0.183694* 0.259533* 0.307507* 0.308709* 0.215527* 0.244778*
Eisosomes 0.000069 0.000041 0.000029 0.000036 0.000124 0.000054 0.000051 0.000021 0.000123 0.000046 0.000039 0.000028
Endoplasmic Reticulum 0.034821 0.001983 0.001458 0.000923 0.003029 0.002258 0.002959 0.000451 0.003744 0.002091 0.002119 0.000681
Endosome 0.338132* 0.322635* 0.378563* 0.283838* 0.337732* 0.369705* 0.400292* 0.302849* 0.337741* 0.341199* 0.388126* 0.293600*
Golgi 0.066967 0.156274* 0.109926 0.084782 0.143896* 0.109443 0.056661 0.059292 0.142166* 0.137804* 0.086483 0.071693
Lipid Particles 0.004653 0.003055 0.010312 0.010634 0.006563 0.003570 0.014202 0.004548 0.006520 0.003258 0.012024 0.007509
Mitochondria 0.007440 0.006585 0.000071 0.001224 0.014416 0.006938 0.000020 0.000710 0.014259 0.006724 0.000048 0.000960
Mitotic Spindle 0.000854 0.003007 0.016461 0.015119 0.002414 0.001893 0.004319 0.007949 0.002379 0.002568 0.011117 0.011437
None 0.000042 0.000057 0.000009 0.000037 0.000471 0.000095 0.000004 0.000013 0.000462 0.000072 0.000007 0.000025
Nuclear Periphery 0.000131 0.000033 0.000135 0.000064 0.000059 0.000050 0.000062 0.000042 0.000060 0.000040 0.000103 0.000053
Nuclear Periphery Foci 0.000475 0.000232 0.002728 0.001318 0.000449 0.000314 0.002283 0.000429 0.000449 0.000264 0.002532 0.000861
Nucleolus 0.000057 0.000053 0.000047 0.000084 0.000417 0.000159 0.000026 0.000063 0.000408 0.000095 0.000038 0.000073
Nucleus 0.000490 0.000207 0.000251 0.000329 0.000880 0.000496 0.000199 0.000283 0.000872 0.000321 0.000228 0.000306
Peroxisomes 0.017303 0.002173 0.006521 0.010202 0.004876 0.002202 0.008562 0.013348 0.005156 0.002184 0.007419 0.011817
Vacuole 0.079020 0.051973 0.151497 0.132101 0.042561 0.074450 0.205121* 0.106285 0.043381 0.060838 0.175099 0.118845
Vacuole Periphery 0.017940 0.036254 0.015613 0.014527 0.030089 0.046193 0.009799 0.009783 0.029815 0.040174 0.013054 0.012091

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.73 -1.70 -4.22 -5.53 -0.28 3.19 -2.47 -1.89 -3.73 1.24 2.42 -2.51 -4.30 -6.51 0.44
Bud Neck 1.75 1.10 0.44 -6.22 -2.04 1.88 -1.02 2.78 0.29 1.49 4.79 -0.11 2.76 -3.76 1.07
Bud Site 0.04 -0.96 -4.09 -10.35 -4.87 0.78 -3.79 -9.23 -9.82 -0.39 0.50 -3.96 -12.30 -14.25 -3.45
Cell Periphery 1.31 1.12 0.56 -5.16 -2.71 1.96 -1.44 1.50 -0.40 1.54 2.89 -1.27 -0.08 -4.61 1.27
Cytoplasm -0.11 1.69 -3.81 -11.54 -14.22 1.95 2.90 -13.62 -14.89 -11.88 1.52 6.07 -15.77 -18.67 -18.70
Cytoplasmic Foci 0.37 2.38 2.90 14.09 1.02 2.24 9.70 7.11 4.69 -5.61 -0.22 8.87 11.07 13.52 -2.60
Eisosomes 1.51 1.96 1.82 0.94 -0.58 5.28 4.77 8.62 5.77 3.09 6.29 6.25 8.02 4.48 1.56
Endoplasmic Reticulum 1.41 1.43 1.45 2.92 1.06 1.29 0.10 4.88 5.53 2.61 2.17 1.84 4.12 5.81 2.82
Endosome 0.34 -0.79 1.39 5.46 5.90 -3.40 -2.80 4.45 7.47 4.89 -0.45 -3.36 6.56 8.53 7.55
Golgi -5.65 -2.28 -0.89 14.15 2.61 6.23 10.90 17.76 11.11 -0.29 0.91 7.56 15.94 19.15 2.34
Lipid Particles 0.65 -1.67 -2.38 -6.90 -0.17 3.65 -2.07 2.27 -1.94 2.62 4.42 -2.51 -1.40 -7.16 1.96
Mitochondria 0.21 1.90 1.60 6.24 -2.15 4.54 9.91 9.27 7.63 -2.40 4.97 9.98 9.18 9.73 -3.05
Mitotic Spindle -2.70 -3.09 -6.28 -5.25 -0.05 0.59 -0.99 -3.49 -3.85 -0.03 -0.22 -2.73 -6.30 -6.36 0.10
None -0.42 0.99 0.07 0.73 -1.81 1.04 1.30 1.27 2.24 -3.68 1.10 1.29 1.24 2.31 -2.35
Nuclear Periphery 1.11 -0.13 0.55 -4.28 2.07 0.49 -0.77 0.86 0.31 1.66 1.66 -2.59 -0.30 -2.95 2.69
Nuclear Periphery Foci 0.86 -3.25 -2.41 -3.55 1.56 1.02 -4.17 -0.90 -1.61 3.63 1.74 -5.06 -2.85 -3.96 3.28
Nucleolus 0.18 0.16 -1.67 -2.39 -1.78 1.21 1.96 1.71 1.21 -2.90 1.61 1.90 1.68 0.40 -2.97
Nucleus 1.57 0.88 0.52 -4.17 -0.72 1.00 1.87 1.45 0.55 -1.28 1.67 1.82 1.47 -0.84 -1.26
Peroxisomes 2.03 1.42 0.97 -10.24 -2.45 4.69 -2.54 -8.06 -12.12 -3.04 5.31 -2.18 -8.08 -15.91 -4.05
Vacuole 1.12 -2.60 -2.41 -15.44 0.90 -8.41 -9.15 -14.37 -6.79 5.26 -6.43 -11.44 -20.46 -16.64 4.39
Vacuole Periphery -2.70 0.36 0.58 12.81 0.60 -6.50 10.78 13.30 17.21 0.02 -5.63 8.78 12.09 21.50 0.78