Standard name
Description Protein involved in prospore membrane morphogenesis; peripheral membrane protein that localizes to the prospore membrane and at numerous membrane contact sites; involved in sporulation, vacuolar protein sorting, prospore membrane formation during sporulation, and protein-Golgi retention; required for mitochondrial integrity; contains a PH-like domain; homologous to human CHAC and COH1 which are involved in Chorea-acanthocytosis and Cohen syndrome, respectively
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.09 0.09 0.06 0 – 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Bud 0 0 0.09 0 0 0 – 0 0.08 0.05 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.72 0.88 0.57 0.35 0.38 0.4 – 0.33 0.34 0.37 0.32 0.44 0.7 0.61 0.62 0.76 0.59 0.73 0.49 0.48 0.27 0.38 0.29 0.29
Endoplasmic Reticulum 0.18 0.06 0.14 0.12 0.08 0.14 – 0.06 0 0 0 0 0.14 0.16 0.13 0.07 0.06 0 0 0 0 0 0.08 0.07
Endosome 0.1 0.06 0.05 0.34 0.39 0.14 – 0.13 0.09 0.06 0 0 0.13 0.29 0.24 0.11 0.19 0.09 0.28 0.24 0.37 0.31 0.39 0.35
Golgi 0 0.06 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0
Mitochondria 0 0 0.17 0.25 0.06 0.36 – 0.43 0.61 0.57 0.75 0.63 0 0 0 0 0 0 0 0 0.11 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.05 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.11 0.09 – 0.13 0.1 0.07 0.08 0.07 0.06 0 0 0.12 0.17 0.13 0.11 0.16 0.13 0.18 0.18 0.2
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 8 0 7 20 21 6 – 15 2 8 2 2 6 5 7 4 9 8 2 0 1 0 0 1
Bud 1 0 7 7 9 8 – 18 35 25 22 21 3 2 0 0 0 0 0 0 0 0 0 2
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 2 0 2 0 1 1
Bud Site 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 0 0 0 1 1 – 0 5 2 2 5 1 0 2 1 4 4 0 0 0 0 0 0
Cytoplasm 159 14 43 80 145 136 – 192 156 193 107 191 109 123 152 100 93 140 120 28 34 21 47 62
Endoplasmic Reticulum 39 1 11 27 31 47 – 33 17 21 8 15 22 32 31 9 10 8 10 2 2 1 14 15
Endosome 21 1 4 79 148 47 – 78 39 33 5 9 20 59 59 14 30 18 70 14 46 17 64 75
Golgi 3 1 1 5 7 6 – 2 0 1 0 0 0 0 1 0 12 8 4 0 0 0 1 4
Mitochondria 3 0 13 57 24 120 – 255 279 299 252 271 1 0 2 1 4 4 4 1 14 1 3 2
Nucleus 1 0 0 0 1 0 – 3 0 1 3 3 0 0 2 0 1 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 2 0 2 – 7 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Nucleolus 0 0 0 1 0 2 – 15 3 4 1 0 0 0 0 0 3 3 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 2 0 4 4 5 3 – 11 8 11 3 5 3 1 0 1 4 0 2 0 2 0 1 2
Vac/Vac Membrane 9 0 1 1 43 31 – 77 46 35 28 29 10 6 10 16 27 26 26 9 16 10 30 41
Unique Cell Count 221 16 76 229 378 336 590 454 520 337 432 155 203 244 132 157 193 248 59 127 56 168 214
Labelled Cell Count 248 17 91 283 435 409 706 590 635 433 552 175 228 266 146 197 219 248 59 127 56 168 214


Cytoplasm, Endosome

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.7 12.1 5.4 6.6 7.6 6.2 – 5.8 5.3 5.1 4.8 4.6 8.9 9.0 9.2 12.8 12.7 12.2 8.0 9.1 9.4
Std Deviation (1e-4) 1.2 1.2 1.2 1.0 1.4 1.0 – 1.1 1.0 0.9 1.4 0.9 1.4 1.2 1.5 2.8 2.9 2.7 1.4 1.5 1.6
Intensity Change (Log2) – – – 0.27 0.49 0.19 – 0.09 -0.04 -0.08 -0.18 -0.24 0.71 0.72 0.76 1.24 1.23 1.17 0.55 0.75 0.78


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.1 0 0 – 0 0 0 0 0 0 0 0 0 -1.0 0
Bud 0 0 0 – 0 -0.4 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -3.3 -2.9 -2.6 – -4.1 -3.7 -3.2 -4.1 -2.0 2.1 0.6 0.9 2.9 0.4 2.5
Endoplasmic Reticulum -0.6 -1.7 -0.1 – -2.9 0 0 0 0 -0.1 0.3 -0.4 -1.8 -2.0 -3.0
Endosome 5.0 5.7 2.1 – 2.0 1.0 0 0 0 1.8 4.2 3.6 1.3 2.8 1.1
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.4 -3.1 3.1 – 4.4 7.2 6.6 0 0 0 0 0 -4.5 -4.0 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 2.7 2.3 – 3.0 2.5 0 2.2 0 0 0 0 2.7 3.5 3.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.6589 6.9169 5.9089 5.0376 4.7929 5.9508 4.5355 6.6779 5.2796 4.7315 4.2206 5.3402 6.4948 8.2403 7.8774 6.868 7.3242 7.9352
Actin 0.0373 0.0007 0.0113 0.0017 0.0011 0.0044 0.0147 0.0002 0.0089 0.0012 0.0215 0.0007 0.0113 0.0001 0.0007 0.002 0.0036 0.0028
Bud 0.0019 0.0062 0.0031 0.0056 0.0003 0.0002 0.0008 0.0001 0.0005 0.0002 0.0008 0.0002 0.0006 0.0003 0.0003 0.0026 0.0005 0.0002
Bud Neck 0.0013 0.0003 0.0015 0.0004 0.0005 0.002 0.0014 0.0002 0.0009 0.0002 0.0007 0.0007 0.0008 0.0002 0.0003 0.0294 0.0003 0.0005
Bud Periphery 0.0025 0.0013 0.0009 0.0022 0.0002 0.0001 0.0008 0 0.0003 0.0001 0.0005 0.0001 0.0005 0.0001 0.0001 0.0049 0.0004 0.0001
Bud Site 0.0011 0.0115 0.0119 0.0008 0.0002 0.0002 0.0043 0.0004 0.0014 0.0002 0.0025 0.0003 0.0061 0.0019 0.0007 0.0242 0.0005 0.0001
Cell Periphery 0.0003 0.0002 0.0003 0.0001 0 0 0.0004 0.0001 0.0004 0 0.0001 0 0.0005 0.0001 0.0001 0.001 0.0001 0
Cytoplasm 0.2115 0.4841 0.3479 0.2487 0.2788 0.3059 0.2377 0.4758 0.3689 0.2736 0.2136 0.3329 0.3404 0.5442 0.493 0.2662 0.2313 0.344
Cytoplasmic Foci 0.0985 0.097 0.0936 0.085 0.1386 0.1257 0.0865 0.0914 0.0906 0.0982 0.1557 0.116 0.0983 0.1149 0.1226 0.0999 0.1245 0.1162
Eisosomes 0.0004 0 0.0001 0 0 0 0.0001 0 0.0001 0 0.0001 0 0.0002 0 0 0 0 0
Endoplasmic Reticulum 0.0235 0.0101 0.012 0.0075 0.0096 0.0169 0.0277 0.0072 0.0112 0.0044 0.0045 0.0125 0.036 0.0062 0.0072 0.0026 0.0125 0.0099
Endosome 0.5059 0.3422 0.4481 0.5433 0.4355 0.4828 0.5324 0.382 0.4304 0.525 0.5308 0.4709 0.3625 0.2869 0.3279 0.4659 0.4813 0.4651
Golgi 0.0324 0.0127 0.0216 0.0209 0.0363 0.0271 0.0302 0.0125 0.03 0.0137 0.029 0.035 0.024 0.0095 0.0177 0.0306 0.049 0.0432
Lipid Particles 0.0071 0.0013 0.0054 0.0122 0.002 0.0017 0.0126 0.001 0.0054 0.0008 0.0055 0.0013 0.0121 0.0007 0.0005 0.0088 0.0017 0.0005
Mitochondria 0.0049 0.0013 0.0027 0.0058 0.0314 0.0082 0.007 0.0005 0.0065 0.0339 0.0123 0.0038 0.0052 0.0005 0.0023 0.0119 0.0604 0.0038
None 0.0089 0.0016 0.0015 0.0028 0.0048 0.0015 0.0014 0.0014 0.0038 0.0004 0.0007 0.0007 0.0421 0.0012 0.0063 0.0006 0.0016 0.0004
Nuclear Periphery 0.0175 0.0016 0.0034 0.007 0.0173 0.0028 0.0072 0.0009 0.0018 0.0019 0.0008 0.0019 0.0067 0.0011 0.001 0.0011 0.0012 0.0009
Nucleolus 0.0002 0.0001 0.0002 0.0002 0.0001 0.0001 0.0002 0 0.0001 0 0.0001 0.0001 0.0007 0.0001 0 0.0022 0 0
Nucleus 0.008 0.0009 0.0014 0.0021 0.0022 0.0008 0.0011 0.0005 0.0006 0.0005 0.0004 0.0011 0.0072 0.0007 0.0006 0.0014 0.0005 0.0003
Peroxisomes 0.0052 0.0005 0.0033 0.0093 0.0126 0.0009 0.0029 0.0003 0.01 0.001 0.0047 0.0017 0.0025 0.0008 0.0006 0.0026 0.0075 0.0026
Punctate Nuclear 0.0016 0.0003 0.001 0.0018 0.0006 0.0004 0.0029 0.0002 0.0005 0.0002 0.0006 0.0004 0.0046 0.0002 0.0003 0.0004 0.0002 0.0001
Vacuole 0.023 0.0218 0.022 0.0301 0.0214 0.0132 0.0217 0.0211 0.0192 0.0329 0.0098 0.0125 0.0325 0.0263 0.0133 0.0322 0.0143 0.0057
Vacuole Periphery 0.0069 0.0045 0.0069 0.0125 0.0066 0.0051 0.0059 0.0041 0.0086 0.0115 0.0053 0.0071 0.0053 0.0041 0.0044 0.0095 0.0084 0.0036

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 33.0378 24.2543 31.8063 43.8604 29.7949 44.1819 38.6879 43.8049 44.9496 45.7611
Translational Efficiency 0.7227 0.6238 0.6942 0.5472 0.651 0.529 0.4956 0.3704 0.4961 0.5184

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘