Standard name | |
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Description | Ubiquitin; becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-26S proteasome system; essential for the cellular stress response; encoded as a polyubiquitin precursor comprised of 5 head-to-tail repeats; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 1 | 9 | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 3 |
Bud | 0 | 0 | 1 | 1 | 1 | 0 | 7 | 13 | 10 | 11 | 19 | 20 | 0 | 1 | 4 | 0 | 0 | 0 | 1 | 5 | 4 | 4 | 32 | 53 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 2 | 0 | 2 | 2 | 9 | 16 | 7 | 22 | 12 | 16 | 1 | 3 | 3 | 5 | 10 | 7 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 117 | 110 | 303 | 327 | 530 | 406 | 454 | 439 | 309 | 347 | 230 | 234 | 491 | 711 | 799 | 414 | 398 | 311 | 118 | 109 | 298 | 348 | 589 | 579 |
Endoplasmic Reticulum | 1 | 0 | 3 | 0 | 12 | 3 | 16 | 21 | 8 | 16 | 4 | 8 | 2 | 2 | 1 | 10 | 13 | 5 | 0 | 0 | 4 | 0 | 1 | 5 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | 1 | 3 | 2 | 1 | 1 | 1 | 2 | 3 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 1 |
Mitochondria | 3 | 5 | 2 | 2 | 3 | 8 | 25 | 22 | 36 | 64 | 63 | 63 | 0 | 0 | 0 | 4 | 2 | 3 | 1 | 1 | 1 | 0 | 1 | 2 |
Nucleus | 0 | 0 | 2 | 1 | 1 | 3 | 11 | 20 | 17 | 14 | 9 | 11 | 3 | 6 | 5 | 1 | 0 | 1 | 0 | 5 | 6 | 4 | 11 | 8 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 5 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 5 | 8 |
Vac/Vac Membrane | 3 | 2 | 18 | 18 | 21 | 56 | 124 | 325 | 163 | 178 | 109 | 146 | 18 | 33 | 19 | 87 | 83 | 71 | 0 | 1 | 3 | 6 | 12 | 5 |
Unique Cell Count | 124 | 114 | 321 | 345 | 561 | 468 | 619 | 824 | 508 | 615 | 406 | 458 | 507 | 737 | 815 | 462 | 448 | 363 | 129 | 127 | 327 | 375 | 669 | 684 |
Labelled Cell Count | 124 | 117 | 332 | 349 | 571 | 478 | 648 | 865 | 557 | 667 | 448 | 507 | 516 | 758 | 833 | 522 | 507 | 402 | 129 | 127 | 327 | 375 | 669 | 684 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.7 | 7.8 | 8.9 | 10.5 | 22.6 | 19.6 | 16.3 | 16.2 | 14.0 | 13.5 | 12.5 | 11.8 | 16.5 | 24.1 | 26.7 | 19.7 | 21.0 | 20.3 | 10.5 | 12.3 | 16.0 |
Std Deviation (1e-4) | 3.5 | 2.0 | 3.3 | 3.8 | 6.3 | 5.4 | 5.2 | 5.9 | 5.2 | 5.5 | 5.1 | 4.5 | 5.4 | 9.2 | 10.4 | 14.1 | 15.6 | 13.7 | 6.4 | 4.2 | 7.6 |
Intensity Change (Log2) | – | – | – | 0.25 | 1.35 | 1.14 | 0.88 | 0.87 | 0.66 | 0.61 | 0.49 | 0.41 | 0.89 | 1.44 | 1.59 | 1.15 | 1.24 | 1.19 | 0.24 | 0.47 | 0.85 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.6 | 3.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 1.6 | 0 | 2.7 | 2.3 | 2.6 | 0 | 0 | 0 | 0 | 1.8 | 0 |
Cytoplasm | 0.2 | 0.1 | -3.5 | -7.7 | -13.1 | -10.7 | -12.0 | -11.4 | -12.9 | 1.7 | 1.6 | 3.2 | -2.4 | -2.7 | -3.8 |
Endoplasmic Reticulum | 0 | 1.3 | 0 | 1.7 | 1.7 | 0 | 1.7 | 0 | 0 | 0 | 0 | 0 | 1.3 | 1.9 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 3.0 | 2.2 | 4.3 | 5.5 | 7.0 | 6.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 2.0 | 2.6 | 1.9 | 0 | 1.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -0.2 | -1.3 | 3.0 | 5.9 | 11.2 | 9.0 | 8.3 | 7.5 | 8.9 | -1.4 | -0.8 | -2.8 | 5.3 | 5.2 | 5.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 8.7545 | 9.5473 | 8.7019 | 9.5446 | 7.0828 | 9.5196 | 6.1181 | 8.0466 | 7.0981 | 6.2209 | 5.3094 | 6.8977 | 5.1476 | 5.0755 | 4.921 | 5.1074 | 4.0164 | 4.7176 |
Actin | 0.0277 | 0.0004 | 0.0104 | 0.0015 | 0.0189 | 0.0032 | 0.0265 | 0.0028 | 0.0006 | 0.0042 | 0.0172 | 0.001 | 0.015 | 0.0014 | 0.0015 | 0.0004 | 0.0183 | 0.0008 |
Bud | 0.001 | 0.0008 | 0.0016 | 0.0004 | 0.0003 | 0.0087 | 0.0017 | 0.0005 | 0.0052 | 0.002 | 0.0006 | 0.0007 | 0.0008 | 0.0033 | 0.0028 | 0.0011 | 0.0033 | 0.0003 |
Bud Neck | 0.0021 | 0.0004 | 0.0021 | 0.0006 | 0.0005 | 0.0022 | 0.0008 | 0.0005 | 0.0004 | 0.0204 | 0.001 | 0.0012 | 0.0022 | 0.0003 | 0.001 | 0.0029 | 0.0023 | 0.0006 |
Bud Periphery | 0.0011 | 0.0002 | 0.0005 | 0.0006 | 0.0003 | 0.0021 | 0.0027 | 0.0003 | 0.0011 | 0.0026 | 0.001 | 0.0004 | 0.0008 | 0.0012 | 0.0009 | 0.0008 | 0.0027 | 0.001 |
Bud Site | 0.0075 | 0.0026 | 0.0187 | 0.0005 | 0.0006 | 0.0004 | 0.0043 | 0.0057 | 0.0003 | 0.0015 | 0.0016 | 0.0001 | 0.0035 | 0.0092 | 0.0082 | 0.0222 | 0.0235 | 0.0002 |
Cell Periphery | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0007 | 0.0002 | 0 | 0.0002 | 0.0002 | 0 | 0.0002 | 0.0002 | 0.0002 | 0.0006 | 0.0002 | 0 |
Cytoplasm | 0.7349 | 0.9567 | 0.8583 | 0.813 | 0.7462 | 0.8657 | 0.7166 | 0.924 | 0.9612 | 0.7801 | 0.7838 | 0.9429 | 0.7139 | 0.9078 | 0.8768 | 0.8782 | 0.6456 | 0.9371 |
Cytoplasmic Foci | 0.0267 | 0.0023 | 0.0146 | 0.003 | 0.025 | 0.0082 | 0.0254 | 0.0112 | 0.0026 | 0.0123 | 0.0104 | 0.0029 | 0.0326 | 0.0079 | 0.0094 | 0.0082 | 0.0843 | 0.0019 |
Eisosomes | 0.0004 | 0 | 0 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0 | 0 | 0.0002 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.009 | 0.0018 | 0.0039 | 0.0083 | 0.0057 | 0.0037 | 0.0053 | 0.0029 | 0.0016 | 0.0073 | 0.0011 | 0.0023 | 0.0067 | 0.0034 | 0.0067 | 0.0038 | 0.0044 | 0.0009 |
Endosome | 0.0374 | 0.0007 | 0.0114 | 0.0164 | 0.0207 | 0.0027 | 0.0327 | 0.0047 | 0.0035 | 0.0609 | 0.0213 | 0.0015 | 0.0505 | 0.0051 | 0.0215 | 0.0127 | 0.0433 | 0.0032 |
Golgi | 0.0091 | 0.0001 | 0.004 | 0.0015 | 0.016 | 0.0007 | 0.0128 | 0.0008 | 0.0001 | 0.0092 | 0.0381 | 0.0001 | 0.0235 | 0.0004 | 0.0033 | 0.0019 | 0.0292 | 0.0009 |
Lipid Particles | 0.0149 | 0 | 0.0007 | 0.0007 | 0.0727 | 0.0056 | 0.03 | 0.0016 | 0 | 0.002 | 0.0128 | 0 | 0.0357 | 0.0003 | 0.0044 | 0.0013 | 0.0175 | 0.0006 |
Mitochondria | 0.0054 | 0.0002 | 0.0008 | 0.0616 | 0.0044 | 0.0024 | 0.0342 | 0.0025 | 0.0004 | 0.0184 | 0.0845 | 0.0002 | 0.0205 | 0.0023 | 0.0014 | 0.0006 | 0.0187 | 0.0272 |
None | 0.0111 | 0.0084 | 0.0128 | 0.0073 | 0.014 | 0.0081 | 0.0047 | 0.0208 | 0.0044 | 0.002 | 0.0014 | 0.0018 | 0.0232 | 0.0286 | 0.0156 | 0.0084 | 0.0023 | 0.0017 |
Nuclear Periphery | 0.0234 | 0.0021 | 0.0057 | 0.019 | 0.0077 | 0.0072 | 0.0064 | 0.0026 | 0.0022 | 0.0208 | 0.002 | 0.0032 | 0.0077 | 0.0037 | 0.0073 | 0.0035 | 0.0026 | 0.0023 |
Nucleolus | 0.001 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0003 | 0.0034 | 0.0004 | 0.0001 | 0.0004 | 0.0004 | 0 | 0.0014 | 0.0004 | 0.0006 | 0.0018 | 0.001 | 0.0001 |
Nucleus | 0.0554 | 0.0214 | 0.0473 | 0.0542 | 0.0246 | 0.0668 | 0.054 | 0.0115 | 0.0141 | 0.0229 | 0.0115 | 0.0392 | 0.0325 | 0.0195 | 0.0259 | 0.0307 | 0.009 | 0.0189 |
Peroxisomes | 0.0202 | 0.0001 | 0.0018 | 0.0004 | 0.0348 | 0.0022 | 0.0184 | 0.0018 | 0.0001 | 0.0042 | 0.0039 | 0.0001 | 0.0153 | 0.0004 | 0.0009 | 0.001 | 0.0824 | 0.0003 |
Punctate Nuclear | 0.0068 | 0.0011 | 0.0032 | 0.0012 | 0.0054 | 0.0085 | 0.0048 | 0.0031 | 0.0008 | 0.0039 | 0.0007 | 0.0016 | 0.0092 | 0.0016 | 0.0027 | 0.0011 | 0.0043 | 0.0003 |
Vacuole | 0.0032 | 0.0006 | 0.0014 | 0.0064 | 0.0012 | 0.001 | 0.0108 | 0.0019 | 0.001 | 0.0201 | 0.0027 | 0.0006 | 0.003 | 0.0027 | 0.0074 | 0.0176 | 0.0035 | 0.0008 |
Vacuole Periphery | 0.0014 | 0.0001 | 0.0004 | 0.0031 | 0.0006 | 0.0003 | 0.0038 | 0.0003 | 0.0002 | 0.0047 | 0.0037 | 0.0001 | 0.0015 | 0.0004 | 0.0013 | 0.0009 | 0.0013 | 0.0008 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 155.9786 | 158.371 | 185.2188 | 177.5451 | 118.5935 | 195.3465 | 218.219 | 255.2124 | 304.6096 | 154.8976 |
Translational Efficiency | 1.8038 | 1.657 | 1.322 | 1.7724 | 1.567 | 2.4544 | 1.842 | 2.1042 | 1.9635 | 2.02 |
Dataset | Hit |
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Protein Abundance | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |