Standard name
Human Ortholog
Description Ubiquitin; becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-26S proteasome system; essential for the cellular stress response; encoded as a polyubiquitin precursor comprised of 5 head-to-tail repeats; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0.05 0 0 0.05 0.08
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.94 0.96 0.94 0.95 0.94 0.87 0.73 0.53 0.61 0.56 0.57 0.51 0.97 0.96 0.98 0.9 0.89 0.86 0.92 0.86 0.91 0.93 0.88 0.85
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.07 0.1 0.16 0.14 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.06 0.05 0 0.12 0.2 0.39 0.32 0.29 0.27 0.32 0 0 0 0.19 0.19 0.2 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 1 2 1 9 2 4 0 0 0 0 0 0 1 0 0 0 0 3
Bud 0 0 1 1 1 0 7 13 10 11 19 20 0 1 4 0 0 0 1 5 4 4 32 53
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 3
Bud Site 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0
Cell Periphery 0 0 2 0 2 2 9 16 7 22 12 16 1 3 3 5 10 7 0 0 0 0 0 0
Cytoplasm 117 110 303 327 530 406 454 439 309 347 230 234 491 711 799 414 398 311 118 109 298 348 589 579
Endoplasmic Reticulum 1 0 3 0 12 3 16 21 8 16 4 8 2 2 1 10 13 5 0 0 4 0 1 5
Endosome 0 0 0 0 0 0 1 0 0 0 0 0 1 1 2 1 1 3 2 1 1 1 2 3
Golgi 0 0 1 0 0 0 0 3 0 0 0 0 0 1 0 0 0 1 0 0 0 2 1 1
Mitochondria 3 5 2 2 3 8 25 22 36 64 63 63 0 0 0 4 2 3 1 1 1 0 1 2
Nucleus 0 0 2 1 1 3 11 20 17 14 9 11 3 6 5 1 0 1 0 5 6 4 11 8
Nuclear Periphery 0 0 0 0 0 0 0 0 2 5 0 3 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 4 3 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 1 5 8
Vac/Vac Membrane 3 2 18 18 21 56 124 325 163 178 109 146 18 33 19 87 83 71 0 1 3 6 12 5
Unique Cell Count 124 114 321 345 561 468 619 824 508 615 406 458 507 737 815 462 448 363 129 127 327 375 669 684
Labelled Cell Count 124 117 332 349 571 478 648 865 557 667 448 507 516 758 833 522 507 402 129 127 327 375 669 684


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.7 7.8 8.9 10.5 22.6 19.6 16.3 16.2 14.0 13.5 12.5 11.8 16.5 24.1 26.7 19.7 21.0 20.3 10.5 12.3 16.0
Std Deviation (1e-4) 3.5 2.0 3.3 3.8 6.3 5.4 5.2 5.9 5.2 5.5 5.1 4.5 5.4 9.2 10.4 14.1 15.6 13.7 6.4 4.2 7.6
Intensity Change (Log2) 0.25 1.35 1.14 0.88 0.87 0.66 0.61 0.49 0.41 0.89 1.44 1.59 1.15 1.24 1.19 0.24 0.47 0.85


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 3.6 3.4 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 1.6 0 2.7 2.3 2.6 0 0 0 0 1.8 0
Cytoplasm 0.2 0.1 -3.5 -7.7 -13.1 -10.7 -12.0 -11.4 -12.9 1.7 1.6 3.2 -2.4 -2.7 -3.8
Endoplasmic Reticulum 0 1.3 0 1.7 1.7 0 1.7 0 0 0 0 0 1.3 1.9 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 3.0 2.2 4.3 5.5 7.0 6.5 0 0 0 0 0 0
Nucleus 0 0 0 0 2.0 2.6 1.9 0 1.9 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.2 -1.3 3.0 5.9 11.2 9.0 8.3 7.5 8.9 -1.4 -0.8 -2.8 5.3 5.2 5.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.7545 9.5473 8.7019 9.5446 7.0828 9.5196 6.1181 8.0466 7.0981 6.2209 5.3094 6.8977 5.1476 5.0755 4.921 5.1074 4.0164 4.7176
Actin 0.0277 0.0004 0.0104 0.0015 0.0189 0.0032 0.0265 0.0028 0.0006 0.0042 0.0172 0.001 0.015 0.0014 0.0015 0.0004 0.0183 0.0008
Bud 0.001 0.0008 0.0016 0.0004 0.0003 0.0087 0.0017 0.0005 0.0052 0.002 0.0006 0.0007 0.0008 0.0033 0.0028 0.0011 0.0033 0.0003
Bud Neck 0.0021 0.0004 0.0021 0.0006 0.0005 0.0022 0.0008 0.0005 0.0004 0.0204 0.001 0.0012 0.0022 0.0003 0.001 0.0029 0.0023 0.0006
Bud Periphery 0.0011 0.0002 0.0005 0.0006 0.0003 0.0021 0.0027 0.0003 0.0011 0.0026 0.001 0.0004 0.0008 0.0012 0.0009 0.0008 0.0027 0.001
Bud Site 0.0075 0.0026 0.0187 0.0005 0.0006 0.0004 0.0043 0.0057 0.0003 0.0015 0.0016 0.0001 0.0035 0.0092 0.0082 0.0222 0.0235 0.0002
Cell Periphery 0.0004 0.0001 0.0002 0.0001 0.0001 0.0001 0.0007 0.0002 0 0.0002 0.0002 0 0.0002 0.0002 0.0002 0.0006 0.0002 0
Cytoplasm 0.7349 0.9567 0.8583 0.813 0.7462 0.8657 0.7166 0.924 0.9612 0.7801 0.7838 0.9429 0.7139 0.9078 0.8768 0.8782 0.6456 0.9371
Cytoplasmic Foci 0.0267 0.0023 0.0146 0.003 0.025 0.0082 0.0254 0.0112 0.0026 0.0123 0.0104 0.0029 0.0326 0.0079 0.0094 0.0082 0.0843 0.0019
Eisosomes 0.0004 0 0 0 0.0002 0 0.0002 0 0 0 0.0002 0 0.0002 0.0001 0 0 0.0001 0
Endoplasmic Reticulum 0.009 0.0018 0.0039 0.0083 0.0057 0.0037 0.0053 0.0029 0.0016 0.0073 0.0011 0.0023 0.0067 0.0034 0.0067 0.0038 0.0044 0.0009
Endosome 0.0374 0.0007 0.0114 0.0164 0.0207 0.0027 0.0327 0.0047 0.0035 0.0609 0.0213 0.0015 0.0505 0.0051 0.0215 0.0127 0.0433 0.0032
Golgi 0.0091 0.0001 0.004 0.0015 0.016 0.0007 0.0128 0.0008 0.0001 0.0092 0.0381 0.0001 0.0235 0.0004 0.0033 0.0019 0.0292 0.0009
Lipid Particles 0.0149 0 0.0007 0.0007 0.0727 0.0056 0.03 0.0016 0 0.002 0.0128 0 0.0357 0.0003 0.0044 0.0013 0.0175 0.0006
Mitochondria 0.0054 0.0002 0.0008 0.0616 0.0044 0.0024 0.0342 0.0025 0.0004 0.0184 0.0845 0.0002 0.0205 0.0023 0.0014 0.0006 0.0187 0.0272
None 0.0111 0.0084 0.0128 0.0073 0.014 0.0081 0.0047 0.0208 0.0044 0.002 0.0014 0.0018 0.0232 0.0286 0.0156 0.0084 0.0023 0.0017
Nuclear Periphery 0.0234 0.0021 0.0057 0.019 0.0077 0.0072 0.0064 0.0026 0.0022 0.0208 0.002 0.0032 0.0077 0.0037 0.0073 0.0035 0.0026 0.0023
Nucleolus 0.001 0.0001 0.0001 0.0003 0.0002 0.0003 0.0034 0.0004 0.0001 0.0004 0.0004 0 0.0014 0.0004 0.0006 0.0018 0.001 0.0001
Nucleus 0.0554 0.0214 0.0473 0.0542 0.0246 0.0668 0.054 0.0115 0.0141 0.0229 0.0115 0.0392 0.0325 0.0195 0.0259 0.0307 0.009 0.0189
Peroxisomes 0.0202 0.0001 0.0018 0.0004 0.0348 0.0022 0.0184 0.0018 0.0001 0.0042 0.0039 0.0001 0.0153 0.0004 0.0009 0.001 0.0824 0.0003
Punctate Nuclear 0.0068 0.0011 0.0032 0.0012 0.0054 0.0085 0.0048 0.0031 0.0008 0.0039 0.0007 0.0016 0.0092 0.0016 0.0027 0.0011 0.0043 0.0003
Vacuole 0.0032 0.0006 0.0014 0.0064 0.0012 0.001 0.0108 0.0019 0.001 0.0201 0.0027 0.0006 0.003 0.0027 0.0074 0.0176 0.0035 0.0008
Vacuole Periphery 0.0014 0.0001 0.0004 0.0031 0.0006 0.0003 0.0038 0.0003 0.0002 0.0047 0.0037 0.0001 0.0015 0.0004 0.0013 0.0009 0.0013 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 155.9786 158.371 185.2188 177.5451 118.5935 195.3465 218.219 255.2124 304.6096 154.8976
Translational Efficiency 1.8038 1.657 1.322 1.7724 1.567 2.4544 1.842 2.1042 1.9635 2.02

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1501 192 2161 844 1578 1403 351 447 3079 1595 2512 1291

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 888.72 1206.12 1314.56 3335.71 909.62 969.16 2419.59 4384.71 899.43 997.68 1468.97 3698.92
Standard Deviation 211.93 470.64 546.84 1744.12 159.65 228.25 780.43 2125.14 187.27 280.06 699.38 1949.74
Intensity Change Log 2 0.440573 0.564779 1.908193 0.091471 1.411427 2.269146 0.274535 1.054114 2.102014

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000305 0.000341 0.000970 0.003914 0.000105 0.000268 0.000472 0.000396 0.000203 0.000277 0.000901 0.002696
Bud Neck 0.002597 0.002251 0.002614 0.008743 0.001779 0.003308 0.003333 0.015365 0.002178 0.003181 0.002715 0.011036
Bud Site 0.005172 0.010197 0.009182 0.024450 0.002702 0.010605 0.004264 0.023421 0.003906 0.010556 0.008495 0.024094
Cell Periphery 0.000120 0.000129 0.000147 0.001017 0.000117 0.000111 0.000514 0.000835 0.000118 0.000113 0.000198 0.000954
Cytoplasm 0.723528* 0.711442* 0.693009* 0.569217* 0.759217* 0.727740* 0.521303* 0.589292* 0.741819* 0.725778* 0.669017* 0.576168*
Cytoplasmic Foci 0.083852 0.083863 0.007271 0.023480 0.053443 0.070857 0.015383 0.037499 0.068267 0.072423 0.008405 0.028334
Eisosomes 0.000034 0.000027 0.000007 0.000017 0.000024 0.000033 0.000009 0.000011 0.000029 0.000032 0.000007 0.000015
Endoplasmic Reticulum 0.001009 0.001112 0.000455 0.000579 0.001167 0.000773 0.000656 0.000334 0.001090 0.000814 0.000483 0.000494
Endosome 0.003793 0.003117 0.000525 0.006786 0.002595 0.006895 0.000933 0.005994 0.003179 0.006441 0.000582 0.006512
Golgi 0.000886 0.003128 0.000184 0.008428 0.000759 0.004632 0.000469 0.004489 0.000821 0.004451 0.000224 0.007064
Lipid Particles 0.001322 0.002177 0.000352 0.003682 0.001060 0.001208 0.001381 0.001379 0.001188 0.001325 0.000496 0.002885
Mitochondria 0.002068 0.001386 0.000485 0.013292 0.000726 0.002127 0.000343 0.019973 0.001380 0.002038 0.000465 0.015605
Mitotic Spindle 0.006970 0.005122 0.014778 0.046718 0.001313 0.010162 0.004881 0.029338 0.004071 0.009555 0.013395 0.040700
None 0.005991 0.004011 0.009763 0.012545 0.006833 0.004064 0.013332 0.014416 0.006422 0.004057 0.010261 0.013192
Nuclear Periphery 0.000201 0.000258 0.000446 0.000997 0.000250 0.000299 0.000767 0.000675 0.000226 0.000294 0.000491 0.000885
Nuclear Periphery Foci 0.002158 0.002515 0.000884 0.001739 0.001473 0.001661 0.002937 0.002193 0.001807 0.001764 0.001171 0.001897
Nucleolus 0.000433 0.000178 0.000167 0.000668 0.000349 0.000555 0.000172 0.003084 0.000390 0.000510 0.000167 0.001504
Nucleus 0.112039 0.071782 0.221780* 0.223489* 0.130770* 0.081206 0.360559* 0.189118* 0.121639 0.080071 0.241171* 0.211588*
Peroxisomes 0.001665 0.001723 0.000164 0.000574 0.000494 0.002278 0.000061 0.000892 0.001065 0.002211 0.000150 0.000684
Vacuole 0.045496 0.095043 0.036657 0.042175 0.034274 0.069504 0.068104 0.059290 0.039744 0.072578 0.041051 0.048101
Vacuole Periphery 0.000361 0.000197 0.000160 0.007489 0.000550 0.001714 0.000128 0.002008 0.000458 0.001531 0.000156 0.005591

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.69 -0.73 -1.52 -1.37 -0.84 -5.54 -11.72 -3.57 1.82 9.26 -1.26 -1.54 -1.83 -1.60 -0.31
Bud Neck -0.17 -0.51 -4.46 -4.71 -4.78 -3.49 -8.94 -4.21 -2.35 1.78 -2.56 -4.41 -6.61 -4.04 -4.18
Bud Site -3.57 -1.88 -4.07 -0.19 -2.80 -5.65 -9.01 -4.96 0.60 -0.28 -5.23 -4.70 -6.34 -0.22 -2.80
Cell Periphery -1.43 -2.07 -10.75 -9.38 -10.36 0.52 -11.36 -9.03 -9.14 1.34 0.13 -8.97 -13.74 -13.76 -10.94
Cytoplasm 3.47 15.07 15.78 4.67 4.73 5.14 28.64 14.96 10.76 -9.72 4.54 26.17 22.57 15.20 2.02
Cytoplasmic Foci 0.11 24.71 18.18 7.31 -9.23 -4.34 8.33 5.64 9.17 -1.68 -0.85 28.81 17.79 14.92 -10.30
Eisosomes 0.30 10.33 9.13 5.51 -1.11 -3.84 3.89 9.97 12.36 6.76 -1.75 12.98 12.34 12.31 0.88
Endoplasmic Reticulum -0.81 4.97 9.49 4.86 8.31 1.79 2.44 5.37 7.74 7.45 2.00 4.33 8.50 8.17 11.64
Endosome 0.89 9.57 5.14 3.19 -1.24 -5.42 5.34 4.64 8.07 0.93 -4.63 11.46 6.19 7.94 -1.11
Golgi -0.68 3.76 -0.76 0.41 -2.03 -3.38 0.65 0.48 3.63 -0.02 -3.48 3.28 -0.59 2.97 -2.19
Lipid Particles -0.72 2.96 -5.40 -1.90 -7.49 -0.72 -8.43 -6.44 -5.42 1.99 -0.43 0.90 -7.33 -6.53 -8.17
Mitochondria 0.88 1.61 0.06 -0.75 -1.32 -1.96 0.80 -0.59 0.23 -0.83 -0.65 1.73 -0.46 -0.03 -1.54
Mitotic Spindle -0.24 -3.35 -3.21 -1.82 -0.89 -3.98 -3.18 -3.85 0.04 0.25 -2.68 -6.09 -4.94 -1.92 -0.30
None 2.29 -2.24 -0.99 -3.70 1.81 3.75 -1.40 -0.56 -4.37 1.02 3.76 -2.72 -1.03 -5.37 2.04
Nuclear Periphery -3.84 -21.52 -8.85 -5.42 1.12 -1.42 -15.54 -9.58 -5.63 9.08 -2.43 -24.99 -11.20 -6.96 3.83
Nuclear Periphery Foci -2.39 4.59 0.20 2.57 -5.70 -1.29 -6.95 -1.79 -0.22 5.39 -0.74 2.18 -1.31 -0.22 -3.90
Nucleolus 1.81 1.71 1.21 -1.31 -1.12 -1.40 1.68 -0.56 0.47 -1.17 -0.87 2.51 0.65 1.36 -1.72
Nucleus 4.44 -27.90 -12.67 -13.82 8.28 10.28 -20.17 -3.78 -9.89 14.93 10.29 -32.61 -12.14 -18.62 14.01
Peroxisomes 0.59 3.09 3.23 3.24 0.73 -3.00 2.73 2.61 3.81 -0.01 -1.70 3.53 3.81 4.11 0.82
Vacuole -6.90 -9.86 -15.42 -1.52 -9.65 -11.66 -11.82 -14.11 -9.41 -2.09 -12.52 -15.90 -21.29 -11.73 -11.08
Vacuole Periphery 1.39 1.95 -1.60 -2.04 -2.19 -1.91 1.36 1.01 2.85 -0.67 -2.21 2.14 -1.07 1.15 -2.29