Standard name
Human Ortholog
Description ER integral membrane protein, ERMES complex subunit; ERMES links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth; required for mitophagy; ERMES complex is often co-localized with peroxisomes and with concentrated areas of pyruvate dehydrogenase
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0.08 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud – 0.05 0.1 0.06 0.07 0.05 0.05 0.07 0.1 0.09 0.14 0.09 – – – 0.09 0.07 0.1 0 0 0 0 0.05 0
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0.05 0.07 0 0.05 0.09 0
Bud Site – 0.05 0 0 0 0.05 0 0 0.07 0.06 0 0.06 – – – 0.06 0.06 0.07 – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Cytoplasm – 0.22 0.28 0.28 0.31 0.29 0.26 0.24 0.23 0.15 0.11 0.12 – – – 0.07 0.07 0.06 0.24 0.1 0.09 0.13 0.21 0.06
Endoplasmic Reticulum – 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Endosome – 0 0.11 0.13 0.09 0.1 0.08 0.07 0.05 0.06 0 0.05 – – – 0.12 0.18 0.16 0.15 0.22 0.15 0.24 0.09 0.17
Golgi – 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Mitochondria – 0.62 0.13 0.21 0.18 0.29 0.28 0.32 0.4 0.44 0.53 0.46 – – – 0.09 0.06 0.07 0 0.09 0.06 0 0.07 0.05
Nucleus – 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nucleolus – 0.05 0 0 0 0.05 0.05 0 0.06 0 0.05 0 – – – 0.06 0.06 0.06 0.05 0 0 0 0 0
Peroxisomes – 0.11 0.24 0.23 0.2 0.16 0.2 0.15 0.15 0.15 0.14 0.22 – – – 0.47 0.45 0.41 0.1 0.16 0.18 0.28 0.08 0.25
SpindlePole – 0.22 0.43 0.39 0.41 0.35 0.43 0.41 0.35 0.4 0.35 0.33 – – – 0.59 0.64 0.61 0.23 0.16 0.32 0.24 0.16 0.19
Vac/Vac Membrane – 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0.06 0.05 0 0 0.06 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 3 2 0 1 0 0 2 0 1 0 2 – – – 1 1 1 0 2 8 0 0 0
Bud – 2 26 13 22 16 24 31 31 37 38 36 – – – 11 21 17 0 1 9 0 1 0
Bud Neck – 0 5 2 1 3 5 12 6 7 4 2 – – – 0 1 0 1 5 10 0 2 0
Bud Site – 2 6 5 8 14 17 20 21 24 12 22 – – – 8 18 13 – – – – – –
Cell Periphery – 0 0 0 0 1 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Cytoplasm – 8 74 63 99 84 116 111 74 61 30 49 – – – 9 22 10 5 8 26 2 5 1
Endoplasmic Reticulum – 0 0 0 0 0 0 0 2 0 1 0 – – – 1 2 1 0 0 0 0 0 0
Endosome – 1 29 29 29 29 34 31 15 22 11 21 – – – 15 58 28 3 17 44 4 2 3
Golgi – 0 0 0 0 0 0 0 0 0 0 0 – – – 0 2 1 0 0 0 0 0 0
Mitochondria – 23 34 47 59 84 125 146 127 177 145 179 – – – 11 18 13 0 7 16 0 2 1
Nucleus – 0 4 8 12 9 16 17 13 8 7 14 – – – 1 2 2 0 1 3 0 0 0
Nuclear Periphery – 0 1 0 0 0 0 1 0 0 0 0 – – – 2 0 0 0 0 1 0 0 0
Nucleolus – 2 7 3 5 15 21 20 19 16 15 13 – – – 7 20 10 1 1 4 0 0 0
Peroxisomes – 4 62 53 66 48 88 67 48 60 39 85 – – – 58 146 73 2 13 53 4 2 5
SpindlePole – 8 112 90 132 103 193 186 110 160 96 131 – – – 73 205 108 5 12 93 4 4 4
Vac/Vac Membrane – 1 6 2 5 4 0 1 0 5 2 4 – – – 0 2 0 1 4 9 0 1 2
Unique Cell Count 37 263 229 323 293 449 457 318 399 273 392 124 321 178 23 81 291 17 28 23
Labelled Cell Count 54 368 315 439 410 639 645 466 578 400 558 197 518 277 23 81 291 17 28 23


Mitochondria, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 4.1 3.7 3.9 3.5 3.4 3.4 3.7 3.3 3.5 3.7 3.8 – – – 5.3 6.2 7.2 – – –
Std Deviation (1e-4) – 1.1 1.0 1.3 1.2 1.3 1.5 1.4 1.3 1.4 1.4 1.4 – – – 1.2 1.4 1.9 – – –
Intensity Change (Log2) – – – 0.08 -0.05 -0.1 -0.1 -0.0 -0.16 -0.07 -0.01 0.04 – – – 0.52 0.76 0.97 – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.003 0.0142 0.0152 0.049 0.0014 0.0089 0.0113 0.0009 0.0187 0.0021 0.0011 0.0087 0.0002 0.0008 0.0004 0.0031 0.0017 0.0009
Bud 0.0003 0.0016 0.001 0.0003 0.0002 0.0009 0.0001 0.0117 0.0005 0 0.0001 0 0 0 0.0001 0 0 0.0001
Bud Neck 0.0005 0.0032 0.0135 0.0147 0.027 0.0216 0.0025 0.0018 0.0217 0.0001 0.0001 0.0025 0.0001 0 0.1657 0.0002 0.0001 0.0003
Bud Periphery 0.0004 0.0035 0.0031 0.0006 0.0005 0.0017 0.0001 0.0101 0.0005 0 0.0002 0.0001 0 0 0.0001 0 0 0.0002
Bud Site 0.0013 0.0269 0.0075 0.0018 0.001 0.0014 0.0007 0.0707 0.0412 0.0003 0.0004 0.0001 0.0003 0 0.0004 0.0001 0.0001 0.0001
Cell Periphery 0.0002 0.0013 0.0004 0.0003 0.0001 0.0005 0.0002 0.0025 0.001 0 0.0001 0.0001 0.0001 0 0.0002 0 0 0.0004
Cytoplasm 0.0314 0.0618 0.0376 0.0122 0.0336 0.0657 0.0499 0.1017 0.0251 0.0007 0.1568 0.0561 0.1055 0 0.1482 0.0032 0.0001 0.1271
Cytoplasmic Foci 0.2497 0.2034 0.2332 0.2085 0.2293 0.2788 0.2212 0.1736 0.3296 0.1675 0.1468 0.353 0.1678 0.1029 0.2289 0.6559 0.1392 0.1393
Eisosomes 0.0005 0.0008 0.0006 0.0004 0.0003 0.0003 0.0012 0.0006 0.0005 0 0.0001 0.0003 0 0 0 0 0 0.0002
Endoplasmic Reticulum 0.0014 0.0011 0.0021 0.0002 0.0005 0.0007 0.0011 0.0027 0.0013 0.0001 0.0021 0.0007 0.0045 0 0.002 0 0 0.0374
Endosome 0.0377 0.0319 0.0355 0.0185 0.0338 0.019 0.0186 0.0065 0.0324 0.0064 0.0296 0.0218 0.1329 0.0045 0.0123 0.0028 0.0042 0.0176
Golgi 0.0073 0.0068 0.0084 0.011 0.005 0.0067 0.0037 0.0031 0.0062 0.0052 0.0063 0.0038 0.0052 0.0162 0.0011 0.0042 0.0104 0.0032
Lipid Particles 0.0687 0.0445 0.0251 0.0221 0.0183 0.0557 0.062 0.0304 0.028 0.0196 0.0239 0.0416 0.0205 0.1399 0.0023 0.1098 0.0943 0.0366
Mitochondria 0.0024 0.0081 0.0103 0.0239 0.0032 0.005 0.0028 0.0055 0.0033 0.0005 0.0017 0.0009 0.001 0.0006 0.0009 0.0004 0.0015 0.0038
None 0.4614 0.4377 0.4712 0.3756 0.5454 0.3654 0.5191 0.4436 0.2817 0.0013 0.5147 0.367 0.5491 0 0.4193 0.0143 0.0001 0.3822
Nuclear Periphery 0.0023 0.0007 0.0039 0.0001 0.0004 0.001 0.0008 0.001 0.001 0.0001 0.0006 0.0008 0.0011 0 0.0007 0 0 0.0028
Nucleolus 0.0024 0.0025 0.003 0.0008 0.0022 0.0017 0.0013 0.0175 0.0101 0.0001 0.0002 0.0002 0.0001 0 0.0002 0.0001 0 0.0003
Nucleus 0.0075 0.0028 0.0031 0.0003 0.0016 0.0026 0.0007 0.0042 0.0026 0.0001 0.0008 0.0005 0.0004 0 0.0009 0 0 0.0005
Peroxisomes 0.0933 0.1227 0.0934 0.2552 0.0861 0.114 0.0912 0.1028 0.1377 0.7763 0.1074 0.1132 0.0041 0.7337 0.0078 0.1991 0.7477 0.2423
Punctate Nuclear 0.0246 0.0179 0.0202 0.0033 0.0061 0.0406 0.0096 0.0047 0.0456 0.0194 0.0014 0.0267 0.0016 0.0011 0.002 0.0066 0.0007 0.0018
Vacuole 0.003 0.0063 0.0096 0.001 0.0035 0.0067 0.0018 0.0042 0.0103 0 0.0051 0.0018 0.0046 0 0.0059 0 0 0.0022
Vacuole Periphery 0.0007 0.0005 0.002 0.0003 0.0004 0.001 0.0002 0.0003 0.0009 0 0.0005 0.0002 0.0008 0 0.0006 0 0 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.7966 9.2401 13.1417 16.6379 17.3103 18.0786 18.8799 22.0697 20.5718 17.9514
Translational Efficiency 0.5935 1.2519 1.094 0.7656 0.6447 0.6862 0.8908 0.5873 0.8181 0.8197

Hit Data

Dataset Hit
Protein Abundance –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘