Standard name
Human Ortholog
Description Histone acetyltransferase; critical for cell survival in presence of DNA damage during S phase, required for recovery after DSB repair; acetylates H3K56, H3K9; H3K56 acetylation activity required for expression homeostasis, buffering of mRNA synthesis rate against changes in gene dosage during S phase; involved in non-homologous end joining and regulation of Ty1 transposition; prevents hyper-amplification of rDNA; interacts physically with Vps75p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.09 0.06 0.07 0.07 0.09 0 0.05 0 0 0 0 0 0.18 0.24 0.26 0.18 0.14 0.17 0 0 0 0.07 0.06 0.07
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.4 0.24 0.1 0.18 0.27 0.51 0.59 0.43 0.43 0.58 0.66 0.54 0 0 0 0 0 0 0 0 0 0 0 0.05
Nucleus 0.76 0.82 0.82 0.81 0.74 0.75 0.69 0.76 0.75 0.76 0.71 0.73 0.78 0.73 0.7 0.79 0.77 0.76 0.7 0.79 0.74 0.6 0.53 0.41
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0.07 0.05 0 0.05 0 0 0 0 0 0 0.05 0 0 0 0 0.08 0.07 0.18 0.1 0.15 0.24 0.24 0.33
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 2 2
Bud 1 0 2 1 0 13 12 5 7 18 9 11 0 0 0 0 0 1 1 5 5 2 9 12
Bud Neck 0 0 1 0 0 0 1 0 0 1 0 0 2 1 1 0 0 0 0 0 0 2 3 4
Bud Site 1 0 1 1 6 13 11 22 18 25 19 25 0 0 0 0 0 0
Cell Periphery 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 14 23 39 25 46 33 42 28 27 18 21 10 99 118 160 68 54 70 5 15 16 31 35 46
Endoplasmic Reticulum 0 2 1 0 1 0 0 0 0 0 0 0 1 2 5 14 12 20 1 0 0 2 3 3
Endosome 0 0 18 8 11 0 0 4 1 3 0 0 2 3 7 3 9 8 2 4 4 3 6 11
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 1 4 1 0 3 1 0 1 3
Mitochondria 64 94 57 68 131 406 498 311 350 474 482 382 2 0 3 9 13 15 4 3 10 12 22 31
Nucleus 122 326 459 302 364 593 587 555 605 622 512 518 430 353 439 291 300 316 135 331 474 264 293 281
Nuclear Periphery 3 0 1 2 3 3 2 5 8 7 8 8 0 0 1 0 0 0 1 0 1 0 1 5
Nucleolus 0 0 0 1 2 4 12 19 14 21 23 27 0 0 0 1 1 2 0 0 2 2 6 11
Peroxisomes 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0
SpindlePole 2 1 3 1 2 0 2 1 4 2 0 3 3 3 3 1 5 1 3 7 15 8 18 28
Vac/Vac Membrane 10 28 28 14 25 27 27 15 24 15 17 36 20 18 19 10 31 30 34 43 94 105 135 226
Unique Cell Count 160 397 561 371 491 794 849 730 809 818 726 706 549 485 625 370 392 414 194 422 640 442 554 687
Labelled Cell Count 217 474 613 423 591 1092 1194 965 1059 1206 1091 1020 559 498 641 399 429 464 194 422 640 442 554 687


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 4.5 4.6 4.4 4.5 3.8 3.9 4.0 3.9 3.8 3.4 3.6 6.0 5.9 5.7 7.1 6.8 7.0 5.9 6.1 6.2
Std Deviation (1e-4) 0.4 0.7 0.9 1.6 2.0 1.2 1.6 1.0 0.8 0.8 0.8 0.8 1.1 0.9 1.1 1.6 1.7 1.9 1.2 1.5 1.5
Intensity Change (Log2) -0.07 -0.01 -0.28 -0.22 -0.21 -0.24 -0.29 -0.41 -0.34 0.39 0.36 0.32 0.63 0.57 0.61 0.37 0.41 0.43


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole -1.7 1.8 1.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.9264 2.3062 2.077 2.2202 1.7668 2.2015 1.9243 1.2526 1.6961 0.9247 1.2838 1.1425 0.6093 0.6294 0.2266 1.1189 0.3719 0.3667
Actin 0.0209 0.0002 0.0016 0.0065 0.0034 0.0005 0.0349 0.0002 0.0226 0.0187 0.0097 0.0008 0.0087 0.0001 0.0001 0.0089 0.0032 0.0023
Bud 0.0002 0.0001 0.0024 0.0001 0.0001 0.0001 0.0011 0.0001 0.0004 0.0003 0.0007 0.0001 0.0035 0.0003 0.0001 0.0003 0.0002 0.0012
Bud Neck 0.0025 0.0005 0.0005 0.0315 0.001 0.0042 0.0072 0.0003 0.0088 0.0019 0.0033 0.0019 0.0099 0.0003 0.0015 0.047 0.001 0.0028
Bud Periphery 0.0005 0.0001 0.0009 0.0002 0.0001 0.0001 0.003 0.0001 0.0007 0.0008 0.0015 0.0001 0.0018 0.0001 0.0001 0.0004 0.0004 0.0006
Bud Site 0.0015 0.0012 0.0007 0.0012 0.0004 0.0003 0.004 0.0005 0.0071 0.0009 0.0019 0.0002 0.0032 0.0009 0.0006 0.0004 0.0004 0.0003
Cell Periphery 0.0002 0.0001 0.0001 0.0006 0.0001 0.0001 0.0008 0 0.0004 0.0001 0.0001 0 0.0002 0 0 0.0001 0.0001 0
Cytoplasm 0.0324 0.05 0.0259 0.0283 0.0261 0.0318 0.0483 0.0618 0.0384 0.017 0.0493 0.0431 0.0235 0.04 0.0179 0.0112 0.044 0.0226
Cytoplasmic Foci 0.0122 0.0012 0.0036 0.0027 0.0035 0.0023 0.0111 0.0011 0.003 0.0015 0.0061 0.0016 0.0104 0.0021 0.0009 0.0008 0.0021 0.0088
Eisosomes 0.0007 0.0001 0.0002 0.0015 0.0002 0 0.0004 0 0.0003 0.0007 0.0001 0 0.0001 0 0 0.0001 0.0001 0
Endoplasmic Reticulum 0.0026 0.0015 0.0029 0.0025 0.0008 0.0008 0.0089 0.0031 0.0021 0.0021 0.0048 0.001 0.0028 0.0007 0.0008 0.0035 0.0032 0.001
Endosome 0.0111 0.0019 0.0084 0.0089 0.0031 0.0019 0.0287 0.0009 0.0051 0.0087 0.0273 0.0018 0.0108 0.0008 0.0008 0.0045 0.0047 0.0012
Golgi 0.0017 0.0001 0.0008 0.0007 0.0005 0.0001 0.0051 0 0.0029 0.0013 0.0039 0.0005 0.0045 0 0 0.0006 0.0008 0.0004
Lipid Particles 0.0042 0.0002 0.0061 0.0012 0.0021 0.0003 0.0101 0.0001 0.001 0.0035 0.0073 0.0045 0.0118 0.0001 0.0002 0.0004 0.001 0.001
Mitochondria 0.003 0.0008 0.0053 0.001 0.0015 0.0004 0.0094 0.0005 0.0012 0.0043 0.0079 0.001 0.0039 0.0002 0.0002 0.001 0.0017 0.0004
None 0.2072 0.142 0.1565 0.1428 0.1159 0.0843 0.0949 0.076 0.0738 0.0177 0.07 0.0388 0.0355 0.0482 0.0265 0.0034 0.1146 0.0314
Nuclear Periphery 0.0277 0.0254 0.016 0.0298 0.012 0.0067 0.0579 0.0068 0.0154 0.0396 0.0352 0.0158 0.024 0.0128 0.0119 0.0269 0.0432 0.0063
Nucleolus 0.0105 0.0134 0.0104 0.0052 0.012 0.0193 0.0187 0.004 0.0043 0.0073 0.0131 0.0093 0.0126 0.0094 0.0066 0.009 0.0067 0.0102
Nucleus 0.6187 0.7105 0.717 0.661 0.7437 0.8129 0.5819 0.8223 0.7841 0.809 0.6859 0.8301 0.7858 0.8405 0.8824 0.8383 0.7561 0.8598
Peroxisomes 0.0092 0.0001 0.001 0.0015 0.0038 0.0001 0.0082 0.0001 0.0007 0.0058 0.0029 0.0019 0.0078 0 0 0.0001 0.0002 0.0006
Punctate Nuclear 0.0298 0.0483 0.0375 0.0715 0.0678 0.0325 0.0555 0.0201 0.0257 0.0562 0.0597 0.0459 0.0363 0.0427 0.0489 0.041 0.008 0.0483
Vacuole 0.0026 0.0021 0.0014 0.0007 0.0015 0.001 0.0065 0.0014 0.0014 0.0013 0.0069 0.0012 0.002 0.0007 0.0004 0.0012 0.0031 0.0005
Vacuole Periphery 0.0007 0.0004 0.0008 0.0005 0.0004 0.0002 0.0035 0.0004 0.0005 0.0013 0.0023 0.0005 0.0008 0.0002 0.0001 0.0009 0.0053 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.358 6.7715 5.583 9.1872 13.6405 9.253 5.8957 6.1613 8.0404 14.1846
Translational Efficiency 0.6433 0.8044 0.6363 0.5453 0.5922 1.4362 0.6964 0.5726 0.5407 0.5002

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Histone acetyltransferase; critical for cell survival in presence of DNA damage during S phase, required for recovery after DSB repair; acetylates H3K56, H3K9; H3K56 acetylation activity required for expression homeostasis, buffering of mRNA synthesis rate against changes in gene dosage during S phase; involved in non-homologous end joining and regulation of Ty1 transposition; prevents hyper-amplification of rDNA; interacts physically with Vps75p
Localization
Cell Percentages nucleus (94%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Rtt109

Rtt109


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rtt109-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available