Standard name
Description Steryl ester hydrolase; catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism; YEH2 has a paralog, YEH1, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.05 0.07 0.08 0.08 0 0 0 0 0 0 0 0 0.16 0.17 0.12 0.09 0.13 0.27 0.08 0.09 0.12 0.13 0.06 0.06
Bud 0 0 0.09 0.1 0.05 0.06 0.05 0.05 0 0.05 0.05 0.08 0.05 0 0 0 0 0.08 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.1 0.1 0.06 0.05 0.06
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.28 0.36 0.23 0.23 0.11 0.14 0.06 0.06 0.12 0.09 0.09 0.09 0.63 0.6 0.53 0.36 0.27 0.31 0.28 0.26 0.41 0.33 0.54 0.44
Cytoplasm 0.83 0.78 0.84 0.85 0.89 0.89 0.83 0.87 0.84 0.78 0.8 0.81 0.62 0.58 0.69 0.64 0.65 0.58 0.17 0.26 0.15 0.17 0.11 0.09
Endoplasmic Reticulum 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.07 0 0.3 0.18 0.09 0.22 0.14 0.22
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.06 0.06 0.08 0.17 0.15 0.22 0.24 0.21 0.28 0 0 0 0 0 0 0 0 0.06 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 10 17 18 14 10 9 8 12 8 6 2 2 30 52 36 13 12 7 19 25 31 16 15 14
Bud 9 10 20 18 16 19 21 25 13 18 14 24 10 11 6 2 2 2 0 1 3 0 3 2
Bud Neck 0 2 3 2 6 0 7 8 6 4 1 3 0 0 0 0 1 0 13 28 26 7 13 14
Bud Site 1 0 1 1 0 0 0 0 0 1 0 1 1 0 0 0 0 0
Cell Periphery 56 91 53 41 35 47 23 29 38 35 24 28 121 187 160 51 25 8 69 70 109 43 138 102
Cytoplasm 168 196 195 152 281 300 340 399 266 297 214 259 119 180 206 89 59 15 41 71 38 22 29 21
Endoplasmic Reticulum 4 12 5 1 3 5 4 2 4 5 9 2 3 5 2 11 6 1 74 50 23 29 36 51
Endosome 2 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 2 1 3 1 4 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0
Mitochondria 6 4 4 10 20 26 68 67 68 91 56 89 2 1 1 4 3 1 10 6 16 2 3 6
Nucleus 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 3 1 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 1 0 0 0 0 0 1 1 0 0 0 0 0 0 0 1 0 0 0 0 0
Nucleolus 1 0 0 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 4 7 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 4 2 0 1 4 4 0 2 1 0 0 3 4 3 0 1 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 1 3 1 1 1 0 0 1 2 0 0 0 0 2 0 9 12 5 5 8 12
Unique Cell Count 203 250 233 178 316 338 411 458 316 381 266 319 192 311 300 140 91 26 244 274 265 131 257 232
Labelled Cell Count 258 332 304 244 375 409 477 547 403 460 323 410 287 443 423 176 112 35 244 274 265 131 257 232


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.2 6.2 4.9 4.6 4.4 4.1 3.6 3.8 3.8 3.5 3.4 3.5 6.3 6.7 6.5 7.4 8.6 9.0 6.0 6.2 6.9
Std Deviation (1e-4) 0.8 1.0 0.8 1.0 1.2 1.0 0.8 0.7 1.1 0.9 1.4 1.0 1.0 1.5 1.1 1.4 1.4 1.5 1.3 1.1 1.2
Intensity Change (Log2) -0.08 -0.15 -0.25 -0.44 -0.37 -0.35 -0.49 -0.49 -0.48 0.38 0.47 0.41 0.6 0.82 0.89 0.3 0.35 0.5


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 2.6 3.1 1.6 0.5 1.5 0
Bud 0 0 0 0 0 0 0 0 0 -1.4 -2.5 -3.5 -2.8 -2.1 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 8.4 8.7 7.2 2.9 0.9 0.9
Cytoplasm 0.5 1.8 1.8 -0.3 1.2 0.2 -1.7 -0.9 -0.8 -5.1 -6.4 -4.0 -4.4 -3.7 -3.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 2.6 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yeh2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available