Standard name | |
---|---|
Description | Steryl ester hydrolase; catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism; YEH2 has a paralog, YEH1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 10 | 17 | 18 | 14 | 10 | 9 | 8 | 12 | 8 | 6 | 2 | 2 | 30 | 52 | 36 | 13 | 12 | 7 | 19 | 25 | 31 | 16 | 15 | 14 |
Bud | 9 | 10 | 20 | 18 | 16 | 19 | 21 | 25 | 13 | 18 | 14 | 24 | 10 | 11 | 6 | 2 | 2 | 2 | 0 | 1 | 3 | 0 | 3 | 2 |
Bud Neck | 0 | 2 | 3 | 2 | 6 | 0 | 7 | 8 | 6 | 4 | 1 | 3 | 0 | 0 | 0 | 0 | 1 | 0 | 13 | 28 | 26 | 7 | 13 | 14 |
Bud Site | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 56 | 91 | 53 | 41 | 35 | 47 | 23 | 29 | 38 | 35 | 24 | 28 | 121 | 187 | 160 | 51 | 25 | 8 | 69 | 70 | 109 | 43 | 138 | 102 |
Cytoplasm | 168 | 196 | 195 | 152 | 281 | 300 | 340 | 399 | 266 | 297 | 214 | 259 | 119 | 180 | 206 | 89 | 59 | 15 | 41 | 71 | 38 | 22 | 29 | 21 |
Endoplasmic Reticulum | 4 | 12 | 5 | 1 | 3 | 5 | 4 | 2 | 4 | 5 | 9 | 2 | 3 | 5 | 2 | 11 | 6 | 1 | 74 | 50 | 23 | 29 | 36 | 51 |
Endosome | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 1 | 3 | 1 | 4 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 |
Mitochondria | 6 | 4 | 4 | 10 | 20 | 26 | 68 | 67 | 68 | 91 | 56 | 89 | 2 | 1 | 1 | 4 | 3 | 1 | 10 | 6 | 16 | 2 | 3 | 6 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 4 | 2 | 0 | 1 | 4 | 4 | 0 | 2 | 1 | 0 | 0 | 3 | 4 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 1 | 3 | 1 | 1 | 1 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 2 | 0 | 9 | 12 | 5 | 5 | 8 | 12 |
Unique Cell Count | 203 | 250 | 233 | 178 | 316 | 338 | 411 | 458 | 316 | 381 | 266 | 319 | 192 | 311 | 300 | 140 | 91 | 26 | 244 | 274 | 265 | 131 | 257 | 232 |
Labelled Cell Count | 258 | 332 | 304 | 244 | 375 | 409 | 477 | 547 | 403 | 460 | 323 | 410 | 287 | 443 | 423 | 176 | 112 | 35 | 244 | 274 | 265 | 131 | 257 | 232 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.2 | 6.2 | 4.9 | 4.6 | 4.4 | 4.1 | 3.6 | 3.8 | 3.8 | 3.5 | 3.4 | 3.5 | 6.3 | 6.7 | 6.5 | 7.4 | 8.6 | 9.0 | 6.0 | 6.2 | 6.9 |
Std Deviation (1e-4) | 0.8 | 1.0 | 0.8 | 1.0 | 1.2 | 1.0 | 0.8 | 0.7 | 1.1 | 0.9 | 1.4 | 1.0 | 1.0 | 1.5 | 1.1 | 1.4 | 1.4 | 1.5 | 1.3 | 1.1 | 1.2 |
Intensity Change (Log2) | – | – | – | -0.08 | -0.15 | -0.25 | -0.44 | -0.37 | -0.35 | -0.49 | -0.49 | -0.48 | 0.38 | 0.47 | 0.41 | 0.6 | 0.82 | 0.89 | 0.3 | 0.35 | 0.5 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.6 | 3.1 | 1.6 | 0.5 | 1.5 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.4 | -2.5 | -3.5 | -2.8 | -2.1 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8.4 | 8.7 | 7.2 | 2.9 | 0.9 | 0.9 |
Cytoplasm | 0.5 | 1.8 | 1.8 | -0.3 | 1.2 | 0.2 | -1.7 | -0.9 | -0.8 | -5.1 | -6.4 | -4.0 | -4.4 | -3.7 | -3.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.6 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |