TheCellVision

YEH2

Standard name	YEH2 ORF YLR020C Aliases sterol esterase
Human Ortholog
Description	Steryl ester hydrolase; catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism; YEH2 has a paralog, YEH1, that arose from the whole genome duplication

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Scale images

YEH2

Micrographs

Sub-cellular Localization

LOC-score
Cell count

	ensLOC																		DeepLoc
Localization	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	WT1	WT2	WT3	AF100	AF140	AF180
Cortical Patches	0.05	0.07	0.08	0.08	0	0	0	0	0	0	0	0	0.16	0.17	0.12	0.09	0.13	0.27	0.08	0.09	0.12	0.13	0.06	0.06
Bud	0	0	0.09	0.1	0.05	0.06	0.05	0.05	0	0.05	0.05	0.08	0.05	0	0	0	0	0.08	0	0	0	0	0	0
Bud Neck	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0.05	0.1	0.1	0.06	0.05	0.06
Bud Site	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	–	–	–	–	–	–
Cell Periphery	0.28	0.36	0.23	0.23	0.11	0.14	0.06	0.06	0.12	0.09	0.09	0.09	0.63	0.6	0.53	0.36	0.27	0.31	0.28	0.26	0.41	0.33	0.54	0.44
Cytoplasm	0.83	0.78	0.84	0.85	0.89	0.89	0.83	0.87	0.84	0.78	0.8	0.81	0.62	0.58	0.69	0.64	0.65	0.58	0.17	0.26	0.15	0.17	0.11	0.09
Endoplasmic Reticulum	0	0.05	0	0	0	0	0	0	0	0	0	0	0	0	0	0.08	0.07	0	0.3	0.18	0.09	0.22	0.14	0.22
Endosome	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Golgi	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Mitochondria	0	0	0	0.06	0.06	0.08	0.17	0.15	0.22	0.24	0.21	0.28	0	0	0	0	0	0	0	0	0.06	0	0	0
Nucleus	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Nuclear Periphery	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Nucleolus	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Peroxisomes	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
SpindlePole	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Vac/Vac Membrane	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0.05

	ensLOC																		DeepLoc
Localization	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	WT1	WT2	WT3	AF100	AF140	AF180
Cortical Patches	10	17	18	14	10	9	8	12	8	6	2	2	30	52	36	13	12	7	19	25	31	16	15	14
Bud	9	10	20	18	16	19	21	25	13	18	14	24	10	11	6	2	2	2	0	1	3	0	3	2
Bud Neck	0	2	3	2	6	0	7	8	6	4	1	3	0	0	0	0	1	0	13	28	26	7	13	14
Bud Site	1	0	1	1	0	0	0	0	0	1	0	1	1	0	0	0	0	0	–	–	–	–	–	–
Cell Periphery	56	91	53	41	35	47	23	29	38	35	24	28	121	187	160	51	25	8	69	70	109	43	138	102
Cytoplasm	168	196	195	152	281	300	340	399	266	297	214	259	119	180	206	89	59	15	41	71	38	22	29	21
Endoplasmic Reticulum	4	12	5	1	3	5	4	2	4	5	9	2	3	5	2	11	6	1	74	50	23	29	36	51
Endosome	2	0	1	0	0	0	0	0	0	0	0	0	0	0	1	0	1	0	2	1	3	1	4	1
Golgi	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	0	0	1	0
Mitochondria	6	4	4	10	20	26	68	67	68	91	56	89	2	1	1	4	3	1	10	6	16	2	3	6
Nucleus	0	0	0	0	0	0	1	0	0	0	0	0	1	0	0	3	1	0	0	0	0	0	0	0
Nuclear Periphery	0	0	0	1	0	0	0	0	0	1	1	0	0	0	0	0	0	0	1	0	0	0	0	0
Nucleolus	1	0	0	1	1	1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Peroxisomes	0	0	0	0	0	0	0	0	0	0	0	0	0	4	7	0	0	0	0	0	0	0	0	0
SpindlePole	1	0	4	2	0	1	4	4	0	2	1	0	0	3	4	3	0	1	0	0	0	0	0	0
Vac/Vac Membrane	0	0	0	1	3	1	1	1	0	0	1	2	0	0	0	0	2	0	9	12	5	5	8	12
Unique Cell Count	203	250	233	178	316	338	411	458	316	381	266	319	192	311	300	140	91	26	244	274	265	131	257	232
Labelled Cell Count	258	332	304	244	375	409	477	547	403	460	323	410	287	443	423	176	112	35	244	274	265	131	257	232

Yeast GFP Assignment

Ambiguous

(Huh et al. Nature 2003 425:686)

Protein Abundance

Screen	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	AF100	AF140	AF180
Mean Cell GFP Intensity (1e^-4)	6.2	6.2	4.9	4.6	4.4	4.1	3.6	3.8	3.8	3.5	3.4	3.5	6.3	6.7	6.5	7.4	8.6	9.0	6.0	6.2	6.9
Std Deviation (1e^-4)	0.8	1.0	0.8	1.0	1.2	1.0	0.8	0.7	1.1	0.9	1.4	1.0	1.0	1.5	1.1	1.4	1.4	1.5	1.3	1.1	1.2
Intensity Change (Log₂)	–	–	–	-0.08	-0.15	-0.25	-0.44	-0.37	-0.35	-0.49	-0.49	-0.48	0.38	0.47	0.41	0.6	0.82	0.89	0.3	0.35	0.5

Localization Change

Localization	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3
Cortical Patches	0	0	0	0	0	0	0	0	0	2.6	3.1	1.6	0.5	1.5	0
Bud	0	0	0	0	0	0	0	0	0	-1.4	-2.5	-3.5	-2.8	-2.1	0
Bud Neck	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Bud Site	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Cell Periphery	0	0	0	0	0	0	0	0	0	8.4	8.7	7.2	2.9	0.9	0.9
Cytoplasm	0.5	1.8	1.8	-0.3	1.2	0.2	-1.7	-0.9	-0.8	-5.1	-6.4	-4.0	-4.4	-3.7	-3.2
Endoplasmic Reticulum	0	0	0	0	0	0	0	0	0	0	0	0	2.6	0	0
Endosome	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Golgi	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Mitochondria	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Nucleus	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Nuclear Periphery	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Nucleolus	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Peroxisomes	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
SpindlePole	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Vacuole	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0

External localization resources

dHITS | Loqate | YeastGFP | YeastRGB | YeastRC

Images

Protein Concentration and Protein Localization Data

	R1						R2						R3
	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase
Concentration	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Actin	0.0003	0.031	0.0046	0.007	0.002	0.0294	0.0567	0.0085	0.0274	0.064	0.0611	0.0065	0.0137	0.0284	0.0026	0.0009	0.0012	0.0018
Bud	0	0.0003	0.0006	0.0003	0.0003	0.0003	0.0007	0.0007	0.0019	0.0009	0.0039	0.0023	0.0004	0.0003	0.0004	0.0002	0.0002	0.0003
Bud Neck	0.0005	0.0042	0.0245	0.0284	0.0433	0.0111	0.0031	0.0064	0.0179	0.0232	0.0067	0.0059	0.001	0.0009	0.0135	0.0012	0.0029	0.0202
Bud Periphery	0.0001	0.0009	0.0058	0.0019	0.0028	0.0018	0.0018	0.0024	0.0152	0.0068	0.0331	0.0162	0.0002	0.0005	0.004	0.0032	0.0048	0.0021
Bud Site	0.0027	0.032	0.0159	0.004	0.0033	0.0009	0.0088	0.1412	0.0594	0.0109	0.007	0.0017	0.002	0.0069	0.0064	0.0021	0.0006	0.0013
Cell Periphery	0.028	0.0387	0.1293	0.0218	0.1225	0.044	0.0851	0.1045	0.1014	0.1629	0.0627	0.1594	0.0003	0.0739	0.1922	0.2086	0.191	0.084
Cytoplasm	0.8814	0.7514	0.674	0.7754	0.729	0.7692	0.3875	0.5222	0.549	0.5294	0.7025	0.6258	0.3602	0.4971	0.6451	0.7266	0.7024	0.7006
Cytoplasmic Foci	0.003	0.009	0.0031	0.0069	0.0017	0.0207	0.0561	0.0148	0.0204	0.007	0.0258	0.0033	0.0098	0.1284	0.0128	0.0016	0.0024	0.0041
Eisosomes	0.0001	0.0011	0.0011	0.0011	0.001	0.0005	0.001	0.0015	0.001	0.0011	0.0007	0.0003	0.0001	0.0007	0.0007	0.0005	0.0004	0.0005
Endoplasmic Reticulum	0.0383	0.0108	0.0151	0.0185	0.0048	0.0057	0.0312	0.0068	0.0048	0.0039	0.0032	0.0029	0.0677	0.0117	0.0082	0.0113	0.0099	0.0302
Endosome	0.0019	0.0029	0.0006	0.0007	0.0002	0.0029	0.0579	0.0137	0.0077	0.0157	0.0399	0.0071	0.0782	0.0095	0.0022	0.0008	0.0003	0.0019
Golgi	0.0002	0.0046	0.0007	0.0006	0.0003	0.0053	0.0154	0.0036	0.0225	0.0207	0.0159	0.0066	0.0024	0.0131	0.0007	0.0003	0.0002	0.0007
Lipid Particles	0.0032	0.0086	0.0066	0.0022	0.002	0.0089	0.0135	0.0025	0.0058	0.0027	0.0043	0.0066	0.0024	0.0442	0.0051	0.0029	0.0033	0.0082
Mitochondria	0	0.0007	0.0001	0.0001	0	0.0017	0.006	0.0003	0.0154	0.0027	0.0203	0.0054	0.0028	0.0037	0.0001	0	0	0.0028
None	0.0343	0.0922	0.1138	0.1271	0.0844	0.0872	0.2002	0.1662	0.1288	0.1421	0.0054	0.1452	0.0233	0.0831	0.0998	0.0347	0.0749	0.1343
Nuclear Periphery	0.001	0.0005	0.0004	0.0003	0.0002	0.0001	0.0181	0.0003	0.0002	0.0003	0.0003	0.0002	0.2399	0.0004	0.0004	0.0005	0.0003	0.0006
Nucleolus	0	0.0002	0.0002	0.0002	0.0001	0.0001	0.0009	0.0003	0.0003	0.0001	0.0002	0.0001	0.0018	0.0002	0.0002	0.0001	0.0001	0.0008
Nucleus	0.0004	0.0003	0.0004	0.0005	0.0003	0.0002	0.0058	0.0005	0.0004	0.0003	0.0004	0.0003	0.1507	0.0002	0.0003	0.0004	0.0003	0.0008
Peroxisomes	0	0.0083	0	0.0002	0	0.0083	0.0147	0.0003	0.0136	0.0007	0.0031	0.0003	0.0005	0.0928	0.0002	0	0	0.0009
Punctate Nuclear	0.0001	0.0006	0.0002	0.0002	0.0001	0.0003	0.0192	0.0003	0.0004	0.0001	0.0003	0.0001	0.0275	0.003	0.0002	0.0001	0.0001	0.0007
Vacuole	0.0043	0.0015	0.0029	0.0025	0.0018	0.0013	0.0106	0.003	0.0063	0.0039	0.003	0.0035	0.0103	0.0008	0.0046	0.0038	0.0046	0.0031
Vacuole Periphery	0.0001	0.0001	0	0	0	0.0001	0.0058	0.0001	0.0002	0.0005	0.0004	0.0003	0.0047	0.0001	0.0001	0	0	0.0002

Sequencing Data

	R1					R2
	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase
Gene Expression	24.5656	14.0301	17.2007	22.3765	26.0996	22.506	18.5032	18.7326	22.2728	26.701
Translational Efficiency	0.9942	0.9053	0.7542	0.6007	0.5248	0.79	0.87	0.465	0.5229	0.5403

Hit Data

Dataset	Hit
Protein Concentration	–
Protein Localization	✘
Gene Expression	✔
Translational Efficiency	✘

Proteome Dynamics

Micrographs

Cell Count

R1				R2				R1 & R2
WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
2529	1611	218	1705	1912	2233	185	2124	4441	3844	403	3829

Protein Abundance

	R1				R2				R1 & R2
	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
Mean	803.77	829.70	1072.74	1032.12	823.33	853.79	1081.53	1081.73	812.19	843.69	1076.78	1059.64
Standard Deviation	89.62	116.52	132.04	127.49	94.13	111.95	126.15	141.72	92.10	114.51	129.45	137.79
Intensity Change Log ₂	—	0.045807	0.416446	0.360756	—	0.052410	0.393531	0.393798	—	0.049152	0.404896	0.377570

Localization Score (DeepLoc)

	R1				R2				R1 & R2
	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
Actin	0.005791	0.019246	0.049359	0.099157	0.009949	0.005218	0.062024	0.094058	0.007581	0.011097	0.055173	0.096329
Bud Neck	0.076447	0.065772	0.031892	0.044298	0.062421	0.076806	0.033267	0.045091	0.070408	0.072182	0.032523	0.044738
Bud Site	0.030952	0.073579	0.093587	0.163290*	0.038260	0.046817	0.148591*	0.162601*	0.034099	0.058033	0.118837*	0.162908*
Cell Periphery	0.083053	0.023467	0.041152	0.134516*	0.104987*	0.005415	0.093862	0.094632	0.092496	0.012981	0.065349	0.112392*
Cytoplasm	0.627846*	0.480790*	0.182936*	0.325665*	0.621788*	0.451718*	0.235153*	0.422250*	0.625238*	0.463902*	0.206907*	0.379242*
Cytoplasmic Foci	0.019329	0.120325	0.123635	0.015151	0.022705	0.128267	0.059773	0.009832	0.020782	0.124938	0.094319	0.012200
Eisosomes	0.062762	0.016594	0.008857	0.041442	0.064978	0.007994	0.024649	0.029936	0.063716	0.011598	0.016106	0.035060
Endoplasmic Reticulum	0.006451	0.010164	0.089443	0.010808	0.003848	0.011545	0.037207	0.005200	0.005330	0.010966	0.065464	0.007697
Endosome	0.003729	0.034490	0.035875	0.003740	0.003030	0.047286	0.008621	0.001916	0.003428	0.041924	0.023364	0.002728
Golgi	0.013514	0.035779	0.014032	0.002596	0.012265	0.026121	0.004008	0.001654	0.012976	0.030169	0.009431	0.002073
Lipid Particles	0.013158	0.029026	0.066099	0.007671	0.008234	0.026690	0.015464	0.005821	0.011038	0.027669	0.042854	0.006645
Mitochondria	0.004530	0.004446	0.000132	0.001828	0.003324	0.004266	0.000058	0.001239	0.004011	0.004341	0.000098	0.001501
Mitotic Spindle	0.000106	0.000244	0.001320	0.001720	0.000281	0.001238	0.000127	0.003168	0.000182	0.000822	0.000772	0.002523
None	0.002118	0.000661	0.000066	0.000182	0.001065	0.001127	0.000041	0.000257	0.001665	0.000932	0.000054	0.000223
Nuclear Periphery	0.000109	0.000083	0.000255	0.000123	0.000090	0.000152	0.000123	0.000075	0.000100	0.000123	0.000195	0.000097
Nuclear Periphery Foci	0.000370	0.000697	0.019060	0.001131	0.000429	0.000651	0.007155	0.000681	0.000395	0.000670	0.013595	0.000881
Nucleolus	0.000216	0.000365	0.000062	0.000052	0.000169	0.000294	0.000045	0.000079	0.000196	0.000324	0.000054	0.000067
Nucleus	0.013564	0.008224	0.021119	0.025610	0.013403	0.018156	0.022114	0.027062	0.013495	0.013994	0.021576	0.026415
Peroxisomes	0.013821	0.012526	0.005265	0.003398	0.009027	0.008633	0.002448	0.003080	0.011757	0.010264	0.003972	0.003222
Vacuole	0.021469	0.061041	0.213713*	0.116640	0.019253	0.128845	0.245131*	0.090810	0.020515	0.100429	0.228136*	0.102312
Vacuole Periphery	0.000666	0.002480	0.002140	0.000980	0.000495	0.002760	0.000137	0.000558	0.000592	0.002643	0.001221	0.000746

Localization Changes (T-score)

	R1					R2					R1 & R2
	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14
Actin	-10.62	-6.84	-24.74*	-20.07*	-6.76	6.48	-6.70	-26.07*	-27.94*	-3.95	-5.35	-9.60	-36.27*	-34.13*	-7.58
Bud Neck	3.15	8.92	11.41	7.51	-2.48	-4.10	6.40	6.72	11.16	-2.66	-0.64	11.07	13.29	13.43	-3.62
Bud Site	-13.90	-7.91	-25.54	-15.56	-7.63	-3.23	-7.99	-26.44*	-24.12	-1.06	-12.23	-10.94	-37.23	-28.36	-5.44
Cell Periphery	21.33*	6.01	-8.75	-20.78	-10.71	30.47*	1.34	3.08	-24.78	-0.07	39.03	4.49	-4.45	-31.90*	-6.46
Cytoplasm	21.09	36.24	44.92	19.39	-10.95	25.11	25.80	28.46	4.09	-12.24	33.61	43.42	50.26	15.62	-17.17
Cytoplasmic Foci	-27.01	-13.50	2.41	27.08	13.83	-32.86*	-6.91	7.87	38.52	9.22	-43.38	-14.46	7.86	46.58	15.95
Eisosomes	24.27*	27.53	9.63	-11.97	-15.54	27.45*	10.18	15.22	-15.97	-1.38	38.16*	22.20	18.26	-19.63	-8.98
Endoplasmic Reticulum	-3.83	-9.10	-4.00	-0.47	8.60	-7.95	-4.56	-2.31	6.25	4.37	-7.86	-9.86	-3.88	4.21	9.46
Endosome	-18.40	-8.60	-0.00	17.74	8.53	-26.43*	-5.61	3.13	26.99	6.61	-32.02*	-9.17	2.20	32.06	9.44
Golgi	-14.76	-0.01	16.73	22.28	6.58	-12.20	10.97	17.15	23.81	3.69	-18.88	3.86	24.67	32.36	7.29
Lipid Particles	-11.41	-8.58	5.81	14.22	9.45	-16.01*	-2.78	3.34	17.76	3.74	-18.64	-8.46	7.45	22.57	9.63
Mitochondria	0.19	12.93	4.38	4.39	-3.07	-1.90	8.60	5.26	9.07	-8.58	-0.97	15.43	7.21	8.70	-5.46
Mitotic Spindle	-1.30	-1.50	-2.94	-2.63	-0.46	-2.01	1.68	-3.22	-1.90	-3.40	-2.30	-1.34	-4.23	-2.73	-2.56
None	4.12	6.44	6.05	3.11	-2.53	-0.13	8.28	6.38	2.13	-8.78	2.39	8.51	7.56	2.90	-6.86
Nuclear Periphery	1.37	-6.96	-0.82	-1.53	4.51	-1.18	-3.24	0.99	1.32	3.49	-0.73	-7.41	-0.12	0.66	5.93
Nuclear Periphery Foci	-4.15	-11.24	-11.55	-4.37	10.77	-1.52	-9.93	-3.82	-0.07	9.53	-2.90	-13.27	-10.89	-1.89	12.75
Nucleolus	-1.36	15.39	16.40	2.56	1.28	-3.64	4.44	1.68	5.28	-1.67	-2.39	10.34	5.14	4.30	-1.32
Nucleus	9.61	-2.54	-10.25	-14.92	-1.21	-6.00	-3.81	-12.50	-8.40	-2.30	-0.98	-4.15	-16.23	-15.39	-2.34
Peroxisomes	1.51	9.05	13.92	16.02	2.51	0.64	11.99	10.21	10.82	-1.63	2.74	13.36	17.14	18.47	1.55
Vacuole	-18.90	-14.68	-24.80	-14.33	6.47	-34.12*	-14.25	-25.04	6.29	9.17	-37.56*	-20.39	-35.04	-3.79	11.32
Vacuole Periphery	-11.07	-0.86	-0.84	4.16	0.67	-12.84	3.53	-0.33	9.71	-2.44	-17.17	-0.66	-0.77	9.23	0.49