Standard name
Human Ortholog
Description Steryl ester hydrolase; catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism; YEH2 has a paralog, YEH1, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.05 0.07 0.08 0.08 0 0 0 0 0 0 0 0 0.16 0.17 0.12 0.09 0.13 0.27 0.08 0.09 0.12 0.13 0.06 0.06
Bud 0 0 0.09 0.1 0.05 0.06 0.05 0.05 0 0.05 0.05 0.08 0.05 0 0 0 0 0.08 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.1 0.1 0.06 0.05 0.06
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.28 0.36 0.23 0.23 0.11 0.14 0.06 0.06 0.12 0.09 0.09 0.09 0.63 0.6 0.53 0.36 0.27 0.31 0.28 0.26 0.41 0.33 0.54 0.44
Cytoplasm 0.83 0.78 0.84 0.85 0.89 0.89 0.83 0.87 0.84 0.78 0.8 0.81 0.62 0.58 0.69 0.64 0.65 0.58 0.17 0.26 0.15 0.17 0.11 0.09
Endoplasmic Reticulum 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.07 0 0.3 0.18 0.09 0.22 0.14 0.22
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.06 0.06 0.08 0.17 0.15 0.22 0.24 0.21 0.28 0 0 0 0 0 0 0 0 0.06 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 10 17 18 14 10 9 8 12 8 6 2 2 30 52 36 13 12 7 19 25 31 16 15 14
Bud 9 10 20 18 16 19 21 25 13 18 14 24 10 11 6 2 2 2 0 1 3 0 3 2
Bud Neck 0 2 3 2 6 0 7 8 6 4 1 3 0 0 0 0 1 0 13 28 26 7 13 14
Bud Site 1 0 1 1 0 0 0 0 0 1 0 1 1 0 0 0 0 0
Cell Periphery 56 91 53 41 35 47 23 29 38 35 24 28 121 187 160 51 25 8 69 70 109 43 138 102
Cytoplasm 168 196 195 152 281 300 340 399 266 297 214 259 119 180 206 89 59 15 41 71 38 22 29 21
Endoplasmic Reticulum 4 12 5 1 3 5 4 2 4 5 9 2 3 5 2 11 6 1 74 50 23 29 36 51
Endosome 2 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 2 1 3 1 4 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0
Mitochondria 6 4 4 10 20 26 68 67 68 91 56 89 2 1 1 4 3 1 10 6 16 2 3 6
Nucleus 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 3 1 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 1 0 0 0 0 0 1 1 0 0 0 0 0 0 0 1 0 0 0 0 0
Nucleolus 1 0 0 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 4 7 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 4 2 0 1 4 4 0 2 1 0 0 3 4 3 0 1 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 1 3 1 1 1 0 0 1 2 0 0 0 0 2 0 9 12 5 5 8 12
Unique Cell Count 203 250 233 178 316 338 411 458 316 381 266 319 192 311 300 140 91 26 244 274 265 131 257 232
Labelled Cell Count 258 332 304 244 375 409 477 547 403 460 323 410 287 443 423 176 112 35 244 274 265 131 257 232


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.2 6.2 4.9 4.6 4.4 4.1 3.6 3.8 3.8 3.5 3.4 3.5 6.3 6.7 6.5 7.4 8.6 9.0 6.0 6.2 6.9
Std Deviation (1e-4) 0.8 1.0 0.8 1.0 1.2 1.0 0.8 0.7 1.1 0.9 1.4 1.0 1.0 1.5 1.1 1.4 1.4 1.5 1.3 1.1 1.2
Intensity Change (Log2) -0.08 -0.15 -0.25 -0.44 -0.37 -0.35 -0.49 -0.49 -0.48 0.38 0.47 0.41 0.6 0.82 0.89 0.3 0.35 0.5


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 2.6 3.1 1.6 0.5 1.5 0
Bud 0 0 0 0 0 0 0 0 0 -1.4 -2.5 -3.5 -2.8 -2.1 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 8.4 8.7 7.2 2.9 0.9 0.9
Cytoplasm 0.5 1.8 1.8 -0.3 1.2 0.2 -1.7 -0.9 -0.8 -5.1 -6.4 -4.0 -4.4 -3.7 -3.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 2.6 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin 0.0003 0.031 0.0046 0.007 0.002 0.0294 0.0567 0.0085 0.0274 0.064 0.0611 0.0065 0.0137 0.0284 0.0026 0.0009 0.0012 0.0018
Bud 0 0.0003 0.0006 0.0003 0.0003 0.0003 0.0007 0.0007 0.0019 0.0009 0.0039 0.0023 0.0004 0.0003 0.0004 0.0002 0.0002 0.0003
Bud Neck 0.0005 0.0042 0.0245 0.0284 0.0433 0.0111 0.0031 0.0064 0.0179 0.0232 0.0067 0.0059 0.001 0.0009 0.0135 0.0012 0.0029 0.0202
Bud Periphery 0.0001 0.0009 0.0058 0.0019 0.0028 0.0018 0.0018 0.0024 0.0152 0.0068 0.0331 0.0162 0.0002 0.0005 0.004 0.0032 0.0048 0.0021
Bud Site 0.0027 0.032 0.0159 0.004 0.0033 0.0009 0.0088 0.1412 0.0594 0.0109 0.007 0.0017 0.002 0.0069 0.0064 0.0021 0.0006 0.0013
Cell Periphery 0.028 0.0387 0.1293 0.0218 0.1225 0.044 0.0851 0.1045 0.1014 0.1629 0.0627 0.1594 0.0003 0.0739 0.1922 0.2086 0.191 0.084
Cytoplasm 0.8814 0.7514 0.674 0.7754 0.729 0.7692 0.3875 0.5222 0.549 0.5294 0.7025 0.6258 0.3602 0.4971 0.6451 0.7266 0.7024 0.7006
Cytoplasmic Foci 0.003 0.009 0.0031 0.0069 0.0017 0.0207 0.0561 0.0148 0.0204 0.007 0.0258 0.0033 0.0098 0.1284 0.0128 0.0016 0.0024 0.0041
Eisosomes 0.0001 0.0011 0.0011 0.0011 0.001 0.0005 0.001 0.0015 0.001 0.0011 0.0007 0.0003 0.0001 0.0007 0.0007 0.0005 0.0004 0.0005
Endoplasmic Reticulum 0.0383 0.0108 0.0151 0.0185 0.0048 0.0057 0.0312 0.0068 0.0048 0.0039 0.0032 0.0029 0.0677 0.0117 0.0082 0.0113 0.0099 0.0302
Endosome 0.0019 0.0029 0.0006 0.0007 0.0002 0.0029 0.0579 0.0137 0.0077 0.0157 0.0399 0.0071 0.0782 0.0095 0.0022 0.0008 0.0003 0.0019
Golgi 0.0002 0.0046 0.0007 0.0006 0.0003 0.0053 0.0154 0.0036 0.0225 0.0207 0.0159 0.0066 0.0024 0.0131 0.0007 0.0003 0.0002 0.0007
Lipid Particles 0.0032 0.0086 0.0066 0.0022 0.002 0.0089 0.0135 0.0025 0.0058 0.0027 0.0043 0.0066 0.0024 0.0442 0.0051 0.0029 0.0033 0.0082
Mitochondria 0 0.0007 0.0001 0.0001 0 0.0017 0.006 0.0003 0.0154 0.0027 0.0203 0.0054 0.0028 0.0037 0.0001 0 0 0.0028
None 0.0343 0.0922 0.1138 0.1271 0.0844 0.0872 0.2002 0.1662 0.1288 0.1421 0.0054 0.1452 0.0233 0.0831 0.0998 0.0347 0.0749 0.1343
Nuclear Periphery 0.001 0.0005 0.0004 0.0003 0.0002 0.0001 0.0181 0.0003 0.0002 0.0003 0.0003 0.0002 0.2399 0.0004 0.0004 0.0005 0.0003 0.0006
Nucleolus 0 0.0002 0.0002 0.0002 0.0001 0.0001 0.0009 0.0003 0.0003 0.0001 0.0002 0.0001 0.0018 0.0002 0.0002 0.0001 0.0001 0.0008
Nucleus 0.0004 0.0003 0.0004 0.0005 0.0003 0.0002 0.0058 0.0005 0.0004 0.0003 0.0004 0.0003 0.1507 0.0002 0.0003 0.0004 0.0003 0.0008
Peroxisomes 0 0.0083 0 0.0002 0 0.0083 0.0147 0.0003 0.0136 0.0007 0.0031 0.0003 0.0005 0.0928 0.0002 0 0 0.0009
Punctate Nuclear 0.0001 0.0006 0.0002 0.0002 0.0001 0.0003 0.0192 0.0003 0.0004 0.0001 0.0003 0.0001 0.0275 0.003 0.0002 0.0001 0.0001 0.0007
Vacuole 0.0043 0.0015 0.0029 0.0025 0.0018 0.0013 0.0106 0.003 0.0063 0.0039 0.003 0.0035 0.0103 0.0008 0.0046 0.0038 0.0046 0.0031
Vacuole Periphery 0.0001 0.0001 0 0 0 0.0001 0.0058 0.0001 0.0002 0.0005 0.0004 0.0003 0.0047 0.0001 0.0001 0 0 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 24.5656 14.0301 17.2007 22.3765 26.0996 22.506 18.5032 18.7326 22.2728 26.701
Translational Efficiency 0.9942 0.9053 0.7542 0.6007 0.5248 0.79 0.87 0.465 0.5229 0.5403

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Steryl ester hydrolase; catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism; YEH2 has a paralog, YEH1, that arose from the whole genome duplication
Localization
Cell Percentages cell periphery (32%), cytoplasm (5%), mixed (57%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Yeh2

Yeh2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yeh2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available