Standard name | |
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Human Ortholog | |
Description | Steryl ester hydrolase; catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism; YEH2 has a paralog, YEH1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 10 | 17 | 18 | 14 | 10 | 9 | 8 | 12 | 8 | 6 | 2 | 2 | 30 | 52 | 36 | 13 | 12 | 7 | 19 | 25 | 31 | 16 | 15 | 14 |
Bud | 9 | 10 | 20 | 18 | 16 | 19 | 21 | 25 | 13 | 18 | 14 | 24 | 10 | 11 | 6 | 2 | 2 | 2 | 0 | 1 | 3 | 0 | 3 | 2 |
Bud Neck | 0 | 2 | 3 | 2 | 6 | 0 | 7 | 8 | 6 | 4 | 1 | 3 | 0 | 0 | 0 | 0 | 1 | 0 | 13 | 28 | 26 | 7 | 13 | 14 |
Bud Site | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 56 | 91 | 53 | 41 | 35 | 47 | 23 | 29 | 38 | 35 | 24 | 28 | 121 | 187 | 160 | 51 | 25 | 8 | 69 | 70 | 109 | 43 | 138 | 102 |
Cytoplasm | 168 | 196 | 195 | 152 | 281 | 300 | 340 | 399 | 266 | 297 | 214 | 259 | 119 | 180 | 206 | 89 | 59 | 15 | 41 | 71 | 38 | 22 | 29 | 21 |
Endoplasmic Reticulum | 4 | 12 | 5 | 1 | 3 | 5 | 4 | 2 | 4 | 5 | 9 | 2 | 3 | 5 | 2 | 11 | 6 | 1 | 74 | 50 | 23 | 29 | 36 | 51 |
Endosome | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 1 | 3 | 1 | 4 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 |
Mitochondria | 6 | 4 | 4 | 10 | 20 | 26 | 68 | 67 | 68 | 91 | 56 | 89 | 2 | 1 | 1 | 4 | 3 | 1 | 10 | 6 | 16 | 2 | 3 | 6 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 4 | 2 | 0 | 1 | 4 | 4 | 0 | 2 | 1 | 0 | 0 | 3 | 4 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 1 | 3 | 1 | 1 | 1 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 2 | 0 | 9 | 12 | 5 | 5 | 8 | 12 |
Unique Cell Count | 203 | 250 | 233 | 178 | 316 | 338 | 411 | 458 | 316 | 381 | 266 | 319 | 192 | 311 | 300 | 140 | 91 | 26 | 244 | 274 | 265 | 131 | 257 | 232 |
Labelled Cell Count | 258 | 332 | 304 | 244 | 375 | 409 | 477 | 547 | 403 | 460 | 323 | 410 | 287 | 443 | 423 | 176 | 112 | 35 | 244 | 274 | 265 | 131 | 257 | 232 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.2 | 6.2 | 4.9 | 4.6 | 4.4 | 4.1 | 3.6 | 3.8 | 3.8 | 3.5 | 3.4 | 3.5 | 6.3 | 6.7 | 6.5 | 7.4 | 8.6 | 9.0 | 6.0 | 6.2 | 6.9 |
Std Deviation (1e-4) | 0.8 | 1.0 | 0.8 | 1.0 | 1.2 | 1.0 | 0.8 | 0.7 | 1.1 | 0.9 | 1.4 | 1.0 | 1.0 | 1.5 | 1.1 | 1.4 | 1.4 | 1.5 | 1.3 | 1.1 | 1.2 |
Intensity Change (Log2) | – | – | – | -0.08 | -0.15 | -0.25 | -0.44 | -0.37 | -0.35 | -0.49 | -0.49 | -0.48 | 0.38 | 0.47 | 0.41 | 0.6 | 0.82 | 0.89 | 0.3 | 0.35 | 0.5 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.6 | 3.1 | 1.6 | 0.5 | 1.5 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.4 | -2.5 | -3.5 | -2.8 | -2.1 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8.4 | 8.7 | 7.2 | 2.9 | 0.9 | 0.9 |
Cytoplasm | 0.5 | 1.8 | 1.8 | -0.3 | 1.2 | 0.2 | -1.7 | -0.9 | -0.8 | -5.1 | -6.4 | -4.0 | -4.4 | -3.7 | -3.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.6 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0003 | 0.031 | 0.0046 | 0.007 | 0.002 | 0.0294 | 0.0567 | 0.0085 | 0.0274 | 0.064 | 0.0611 | 0.0065 | 0.0137 | 0.0284 | 0.0026 | 0.0009 | 0.0012 | 0.0018 |
Bud | 0 | 0.0003 | 0.0006 | 0.0003 | 0.0003 | 0.0003 | 0.0007 | 0.0007 | 0.0019 | 0.0009 | 0.0039 | 0.0023 | 0.0004 | 0.0003 | 0.0004 | 0.0002 | 0.0002 | 0.0003 |
Bud Neck | 0.0005 | 0.0042 | 0.0245 | 0.0284 | 0.0433 | 0.0111 | 0.0031 | 0.0064 | 0.0179 | 0.0232 | 0.0067 | 0.0059 | 0.001 | 0.0009 | 0.0135 | 0.0012 | 0.0029 | 0.0202 |
Bud Periphery | 0.0001 | 0.0009 | 0.0058 | 0.0019 | 0.0028 | 0.0018 | 0.0018 | 0.0024 | 0.0152 | 0.0068 | 0.0331 | 0.0162 | 0.0002 | 0.0005 | 0.004 | 0.0032 | 0.0048 | 0.0021 |
Bud Site | 0.0027 | 0.032 | 0.0159 | 0.004 | 0.0033 | 0.0009 | 0.0088 | 0.1412 | 0.0594 | 0.0109 | 0.007 | 0.0017 | 0.002 | 0.0069 | 0.0064 | 0.0021 | 0.0006 | 0.0013 |
Cell Periphery | 0.028 | 0.0387 | 0.1293 | 0.0218 | 0.1225 | 0.044 | 0.0851 | 0.1045 | 0.1014 | 0.1629 | 0.0627 | 0.1594 | 0.0003 | 0.0739 | 0.1922 | 0.2086 | 0.191 | 0.084 |
Cytoplasm | 0.8814 | 0.7514 | 0.674 | 0.7754 | 0.729 | 0.7692 | 0.3875 | 0.5222 | 0.549 | 0.5294 | 0.7025 | 0.6258 | 0.3602 | 0.4971 | 0.6451 | 0.7266 | 0.7024 | 0.7006 |
Cytoplasmic Foci | 0.003 | 0.009 | 0.0031 | 0.0069 | 0.0017 | 0.0207 | 0.0561 | 0.0148 | 0.0204 | 0.007 | 0.0258 | 0.0033 | 0.0098 | 0.1284 | 0.0128 | 0.0016 | 0.0024 | 0.0041 |
Eisosomes | 0.0001 | 0.0011 | 0.0011 | 0.0011 | 0.001 | 0.0005 | 0.001 | 0.0015 | 0.001 | 0.0011 | 0.0007 | 0.0003 | 0.0001 | 0.0007 | 0.0007 | 0.0005 | 0.0004 | 0.0005 |
Endoplasmic Reticulum | 0.0383 | 0.0108 | 0.0151 | 0.0185 | 0.0048 | 0.0057 | 0.0312 | 0.0068 | 0.0048 | 0.0039 | 0.0032 | 0.0029 | 0.0677 | 0.0117 | 0.0082 | 0.0113 | 0.0099 | 0.0302 |
Endosome | 0.0019 | 0.0029 | 0.0006 | 0.0007 | 0.0002 | 0.0029 | 0.0579 | 0.0137 | 0.0077 | 0.0157 | 0.0399 | 0.0071 | 0.0782 | 0.0095 | 0.0022 | 0.0008 | 0.0003 | 0.0019 |
Golgi | 0.0002 | 0.0046 | 0.0007 | 0.0006 | 0.0003 | 0.0053 | 0.0154 | 0.0036 | 0.0225 | 0.0207 | 0.0159 | 0.0066 | 0.0024 | 0.0131 | 0.0007 | 0.0003 | 0.0002 | 0.0007 |
Lipid Particles | 0.0032 | 0.0086 | 0.0066 | 0.0022 | 0.002 | 0.0089 | 0.0135 | 0.0025 | 0.0058 | 0.0027 | 0.0043 | 0.0066 | 0.0024 | 0.0442 | 0.0051 | 0.0029 | 0.0033 | 0.0082 |
Mitochondria | 0 | 0.0007 | 0.0001 | 0.0001 | 0 | 0.0017 | 0.006 | 0.0003 | 0.0154 | 0.0027 | 0.0203 | 0.0054 | 0.0028 | 0.0037 | 0.0001 | 0 | 0 | 0.0028 |
None | 0.0343 | 0.0922 | 0.1138 | 0.1271 | 0.0844 | 0.0872 | 0.2002 | 0.1662 | 0.1288 | 0.1421 | 0.0054 | 0.1452 | 0.0233 | 0.0831 | 0.0998 | 0.0347 | 0.0749 | 0.1343 |
Nuclear Periphery | 0.001 | 0.0005 | 0.0004 | 0.0003 | 0.0002 | 0.0001 | 0.0181 | 0.0003 | 0.0002 | 0.0003 | 0.0003 | 0.0002 | 0.2399 | 0.0004 | 0.0004 | 0.0005 | 0.0003 | 0.0006 |
Nucleolus | 0 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0009 | 0.0003 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0018 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0008 |
Nucleus | 0.0004 | 0.0003 | 0.0004 | 0.0005 | 0.0003 | 0.0002 | 0.0058 | 0.0005 | 0.0004 | 0.0003 | 0.0004 | 0.0003 | 0.1507 | 0.0002 | 0.0003 | 0.0004 | 0.0003 | 0.0008 |
Peroxisomes | 0 | 0.0083 | 0 | 0.0002 | 0 | 0.0083 | 0.0147 | 0.0003 | 0.0136 | 0.0007 | 0.0031 | 0.0003 | 0.0005 | 0.0928 | 0.0002 | 0 | 0 | 0.0009 |
Punctate Nuclear | 0.0001 | 0.0006 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0.0192 | 0.0003 | 0.0004 | 0.0001 | 0.0003 | 0.0001 | 0.0275 | 0.003 | 0.0002 | 0.0001 | 0.0001 | 0.0007 |
Vacuole | 0.0043 | 0.0015 | 0.0029 | 0.0025 | 0.0018 | 0.0013 | 0.0106 | 0.003 | 0.0063 | 0.0039 | 0.003 | 0.0035 | 0.0103 | 0.0008 | 0.0046 | 0.0038 | 0.0046 | 0.0031 |
Vacuole Periphery | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0058 | 0.0001 | 0.0002 | 0.0005 | 0.0004 | 0.0003 | 0.0047 | 0.0001 | 0.0001 | 0 | 0 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 24.5656 | 14.0301 | 17.2007 | 22.3765 | 26.0996 | 22.506 | 18.5032 | 18.7326 | 22.2728 | 26.701 |
Translational Efficiency | 0.9942 | 0.9053 | 0.7542 | 0.6007 | 0.5248 | 0.79 | 0.87 | 0.465 | 0.5229 | 0.5403 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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