Standard name
Human Ortholog
Description Guanine nucleotide exchange factor (GEF) for Ria1p; essential protein involved in ribosome maturation; with Ria1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; ortholog of the human protein (SBDS) responsible for autosomal recessive Shwachman-Bodian-Diamond Syndrome; highly conserved across archaea and eukaryotes

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0.11 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.99 0.99 0.98 0.96 0.93 0.91 0.78 0.73 0.8 0.64 0.99 0.99 1.0 0.98 0.94 0.95 0.92 0.96 0.96 0.93 0.97 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.1 0.2 0.26 0.19 0.3 0 0 0 0 0.05 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.07 0.07 0.07 0.1 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 1 0 0 0 0 3 0 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 5 5 5 12 3 1 0 5 2 4 4 4 8 3 8 9 0 0 0 0 0 0
Cytoplasm 127 405 195 149 109 186 197 166 166 153 98 171 220 292 234 226 240 129 390 184 120 193 167
Endoplasmic Reticulum 1 3 2 2 1 3 2 3 0 1 1 2 2 0 11 5 8 0 2 1 0 0 1
Endosome 0 0 0 1 0 0 0 1 0 0 0 0 0 0 1 1 2 0 2 0 1 0 0
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2 0 1 0 0 0 0
Mitochondria 1 1 0 0 1 3 21 42 59 36 46 0 0 0 6 11 3 0 0 0 0 0 1
Nucleus 5 0 1 0 1 1 6 8 10 9 7 1 2 0 2 5 10 1 3 1 1 0 0
Nuclear Periphery 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1
Nucleolus 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 2 5 2 15 17 13 15 0 0 0 4 6 5 4 3 0 1 1 1
Unique Cell Count 131 410 197 152 113 199 217 213 228 192 154 173 222 293 240 240 253 140 408 193 129 200 179
Labelled Cell Count 134 416 203 157 126 203 232 236 258 215 172 178 228 300 262 264 279 140 408 193 129 200 179


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.3 10.5 11.5 10.2 9.8 7.8 4.5 6.2 6.0 6.1 6.1 11.1 10.5 10.0 8.8 10.9 11.6 11.8 12.1 12.1
Std Deviation (1e-4) 2.5 1.8 1.7 1.3 1.6 1.5 1.1 1.2 1.1 1.1 1.2 1.6 1.4 1.4 1.7 2.2 2.7 1.9 1.8 2.0
Intensity Change (Log2) -0.18 -0.23 -0.56 -1.35 -0.9 -0.93 -0.91 -0.91 -0.05 -0.13 -0.2 -0.38 -0.07 0.01 0.04 0.07 0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 3.0 0 0 0 0 0 0 0 0 0.1 0 0.5 0.6
Cytoplasm -0.7 -1.6 -2.9 -3.7 -6.6 -7.5 -6.2 -8.8 -0.1 0.1 0.9 -1.2 -2.7 -2.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 2.2 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 6.6 7.7 6.4 8.2 0 0 0 0 0 0
Nucleus 0 0 0 0 0 2.5 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 3.8 3.9 3.7 4.5 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.9807 4.5678 4.6803 4.2453 4.3808 4.6998 7.8527 9.5259 9.3914 8.0972 10.0957 8.99 5.4413 7.2529 6.4564 6.8603 6.1207 6.4708
Actin 0.0164 0.0005 0.024 0.0089 0.0001 0.0016 0.0486 0.0003 0.0086 0.0094 0.0005 0.0078 0.0178 0.0043 0.0184 0.0103 0.0268 0.0034
Bud 0.0043 0.0014 0.0009 0.0045 0.0001 0.0003 0.001 0.0001 0.0027 0.0021 0.0001 0.0003 0.0006 0.0002 0.0005 0.0009 0.0007 0.0001
Bud Neck 0.0086 0.0002 0.0005 0.0012 0.0005 0.0009 0.0009 0.0001 0.0006 0.0011 0.0006 0.0012 0.0008 0.0001 0.0007 0.0033 0.0009 0.0005
Bud Periphery 0.003 0.0006 0.0013 0.0014 0.0001 0.0002 0.001 0 0.0015 0.0013 0 0.0004 0.0006 0.0001 0.0003 0.001 0.0003 0.0001
Bud Site 0.0055 0.0029 0.0018 0.0049 0.0001 0.0002 0.0021 0.0005 0.0103 0.0082 0.0001 0.0002 0.0019 0.0047 0.0051 0.0021 0.0074 0.0002
Cell Periphery 0.0002 0.0001 0.0002 0.0002 0 0 0.0008 0.0001 0.0004 0.0005 0 0 0.0003 0.0001 0.0002 0.0002 0.0001 0
Cytoplasm 0.7727 0.9008 0.8369 0.8889 0.9197 0.9339 0.6116 0.9603 0.8617 0.8655 0.8791 0.91 0.7399 0.9626 0.9051 0.7886 0.8505 0.9321
Cytoplasmic Foci 0.0306 0.0099 0.0169 0.0184 0.0115 0.0122 0.0398 0.0103 0.0117 0.0169 0.0038 0.0113 0.0272 0.0026 0.0114 0.0352 0.0181 0.007
Eisosomes 0.0001 0 0.0002 0.0001 0 0 0.0005 0 0.0001 0.0001 0 0.0001 0.0002 0 0.0001 0.0001 0.0001 0
Endoplasmic Reticulum 0.0095 0.0034 0.0022 0.0026 0.0012 0.0055 0.012 0.0038 0.006 0.0058 0.0026 0.0021 0.0164 0.0016 0.0034 0.0051 0.0025 0.0028
Endosome 0.042 0.0099 0.0223 0.0191 0.0152 0.0128 0.0474 0.0041 0.0244 0.0279 0.0043 0.0083 0.0515 0.0008 0.0184 0.0569 0.0246 0.0133
Golgi 0.0043 0.0005 0.0068 0.0035 0.0024 0.0055 0.0243 0.0005 0.0041 0.0071 0.0001 0.0051 0.0173 0.0006 0.0043 0.0352 0.0317 0.0084
Lipid Particles 0.0029 0.0001 0.0021 0.0033 0 0.0014 0.018 0.0108 0.0014 0.0005 0 0.0005 0.0119 0.0001 0.0029 0.0047 0.013 0.0016
Mitochondria 0.0049 0.0004 0.0185 0.0012 0.0358 0.002 0.0104 0.0001 0.0044 0.009 0.0003 0.0182 0.0157 0.0004 0.003 0.014 0.0021 0.0102
None 0.0497 0.0592 0.0528 0.0262 0.0053 0.0172 0.0947 0.0057 0.0421 0.0037 0.0047 0.0307 0.0421 0.0202 0.0096 0.0125 0.0106 0.0139
Nuclear Periphery 0.0164 0.0014 0.0013 0.0015 0.0006 0.0011 0.0183 0.0007 0.0064 0.0017 0.0438 0.0006 0.0214 0.0002 0.0036 0.0071 0.0005 0.0004
Nucleolus 0.0006 0.0001 0.0001 0.0002 0 0.0001 0.0014 0.0001 0.0003 0.0001 0.0002 0 0.0011 0 0.0004 0.0003 0 0
Nucleus 0.0118 0.0037 0.0022 0.0039 0.0026 0.0016 0.0231 0.0011 0.0069 0.0027 0.0544 0.0011 0.0146 0.0005 0.0066 0.002 0.0012 0.0007
Peroxisomes 0.0036 0.0001 0.0018 0.0027 0.0003 0.0007 0.0226 0.0002 0.0011 0.0014 0 0.0003 0.0055 0.0005 0.0033 0.0067 0.0069 0.0039
Punctate Nuclear 0.0041 0.0006 0.0016 0.0022 0.0004 0.0006 0.0153 0.0006 0.0019 0.0005 0.0038 0.0008 0.0052 0.0001 0.0004 0.0038 0.0007 0.0002
Vacuole 0.0062 0.0036 0.0039 0.0042 0.0034 0.0017 0.0045 0.0006 0.0026 0.0332 0.0008 0.0006 0.0052 0.0003 0.0015 0.0056 0.0009 0.0008
Vacuole Periphery 0.0028 0.0005 0.0018 0.0009 0.0008 0.0004 0.0016 0.0001 0.0007 0.0014 0.0007 0.0004 0.0028 0 0.0005 0.0045 0.0003 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 28.5742 23.5787 32.0768 34.426 40.8475 14.6453 27.1516 26.8178 27.764 36.7712
Translational Efficiency 2.4144 2.4152 1.7691 1.6305 1.6359 4.3436 1.4928 1.6864 1.5824 1.5234

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
949 650 198 1185 2050 2057 625 1756 2999 2707 823 2941

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 754.09 1197.14 1375.02 1243.88 982.35 1134.45 1276.58 1235.31 910.12 1149.50 1300.26 1238.76
Standard Deviation 117.29 168.93 163.63 188.57 138.30 140.32 195.62 224.60 169.40 150.10 193.06 210.87
Intensity Change Log 2 0.666783 0.866644 0.722039 0.207684 0.377975 0.330564 0.425182 0.610731 0.513736

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000587 0.000324 0.000492 0.003410 0.000116 0.000129 0.002040 0.000133 0.000265 0.000176 0.001667 0.001453
Bud Neck 0.005499 0.000947 0.001178 0.003200 0.001085 0.003336 0.001032 0.002241 0.002482 0.002762 0.001067 0.002627
Bud Site 0.020293 0.016055 0.021193 0.032866 0.007565 0.008840 0.005658 0.011639 0.011592 0.010572 0.009395 0.020192
Cell Periphery 0.000196 0.000118 0.000214 0.000237 0.000093 0.000056 0.000076 0.000088 0.000126 0.000071 0.000109 0.000148
Cytoplasm 0.654897* 0.799119* 0.793682* 0.671267* 0.802276* 0.650195* 0.778682* 0.787373* 0.755640* 0.685954* 0.782290* 0.740591*
Cytoplasmic Foci 0.180230* 0.109186 0.063932 0.042786 0.120324 0.075958 0.020930 0.017342 0.139281 0.083936 0.031276 0.027594
Eisosomes 0.000074 0.000010 0.000013 0.000014 0.000012 0.000014 0.000011 0.000016 0.000032 0.000013 0.000011 0.000015
Endoplasmic Reticulum 0.002181 0.002044 0.000953 0.002105 0.001470 0.001227 0.000845 0.000278 0.001695 0.001423 0.000871 0.001014
Endosome 0.013408 0.007791 0.003971 0.009238 0.004915 0.011646 0.002288 0.002743 0.007603 0.010721 0.002693 0.005360
Golgi 0.006569 0.005250 0.000556 0.009438 0.004233 0.001885 0.000176 0.003611 0.004973 0.002693 0.000267 0.005959
Lipid Particles 0.002726 0.000782 0.001669 0.001106 0.000898 0.000950 0.000697 0.000404 0.001477 0.000910 0.000931 0.000687
Mitochondria 0.002857 0.001727 0.000017 0.002977 0.000352 0.001139 0.000047 0.000363 0.001145 0.001280 0.000040 0.001417
Mitotic Spindle 0.000685 0.004230 0.001271 0.037116 0.000186 0.002469 0.003242 0.010319 0.000344 0.002892 0.002768 0.021116
None 0.007014 0.001087 0.001239 0.004137 0.002429 0.001751 0.004375 0.003777 0.003880 0.001592 0.003621 0.003922
Nuclear Periphery 0.000297 0.000033 0.000129 0.000581 0.000029 0.000152 0.000234 0.000174 0.000114 0.000123 0.000208 0.000338
Nuclear Periphery Foci 0.001703 0.002038 0.006923 0.003093 0.000637 0.000324 0.004572 0.001814 0.000974 0.000736 0.005137 0.002329
Nucleolus 0.001466 0.000112 0.000059 0.000329 0.000129 0.000393 0.000165 0.000353 0.000552 0.000326 0.000139 0.000343
Nucleus 0.031217 0.007240 0.023646 0.027373 0.013284 0.037464 0.060529 0.045632 0.018959 0.030207 0.051655 0.038275
Peroxisomes 0.001610 0.000702 0.000260 0.001037 0.000411 0.001106 0.000314 0.000225 0.000790 0.001009 0.000301 0.000552
Vacuole 0.062378 0.038725 0.078442 0.130007 0.037411 0.198107 0.113753 0.108586 0.045312 0.159837 0.105258 0.117217
Vacuole Periphery 0.004110 0.002481 0.000162 0.017685 0.002144 0.002859 0.000336 0.002890 0.002766 0.002768 0.000294 0.008851

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.93 0.53 -1.03 -2.12 -1.76 -0.35 -1.06 -1.07 -0.24 1.04 0.97 -0.96 -1.45 -2.36 0.64
Bud Neck 5.09 4.47 4.03 -4.62 -1.62 -4.70 -2.61 -2.83 2.73 -0.81 -0.61 2.83 1.87 2.28 -1.26
Bud Site 1.93 0.86 3.21 1.07 0.62 -1.14 1.76 -1.82 -0.54 -2.69 1.14 2.27 0.44 -0.59 -1.70
Cell Periphery 3.65 0.35 4.05 1.19 3.50 6.29 2.91 3.17 -1.36 0.85 6.88 2.52 4.72 -1.28 2.27
Cytoplasm -11.87 -4.71 3.10 15.13 6.77 20.65 9.16 4.13 -14.95 -6.19 10.55 4.70 7.00 -3.56 -0.04
Cytoplasmic Foci 9.12 14.10 25.93 15.59 5.96 11.26 28.55 30.99 22.78 2.36 15.27 31.31 39.50 26.76 5.19
Eisosomes 3.00 2.91 2.93 -1.57 0.00 -2.26 -0.24 0.43 2.08 0.56 2.71 2.86 2.95 1.81 0.48
Endoplasmic Reticulum 0.17 4.01 4.91 1.34 0.23 1.12 4.11 6.46 6.96 3.69 0.93 5.85 7.82 3.71 2.28
Endosome 3.38 9.17 9.80 3.41 0.55 -8.93 8.21 9.06 13.34 0.60 -4.05 12.37 12.61 12.72 0.34
Golgi 0.96 7.00 4.05 2.35 -1.72 5.54 24.32 3.55 0.08 -2.43 4.55 15.81 5.38 1.29 -3.06
Lipid Particles 5.63 2.36 4.32 -4.29 2.02 -0.48 0.44 5.50 5.36 4.08 4.46 2.87 5.54 1.29 2.88
Mitochondria 0.93 4.35 3.15 0.99 -2.09 -3.06 8.05 1.88 3.55 -2.45 -0.30 5.30 3.19 2.62 -2.75
Mitotic Spindle -2.25 -2.00 -4.67 -2.60 -3.15 -3.03 -1.86 -5.16 -2.88 -1.96 -3.70 -2.27 -6.94 -4.14 -3.33
None 3.43 3.32 2.98 -1.67 -1.21 1.32 -0.83 -0.33 -1.24 0.42 3.40 1.76 2.36 -1.38 0.48
Nuclear Periphery 2.81 0.94 -1.05 -8.28 -3.91 -5.14 -13.95 -4.81 -0.42 4.49 -0.25 -5.32 -3.84 -4.57 1.12
Nuclear Periphery Foci -0.66 -5.65 -1.86 -1.85 5.22 5.61 -8.68 -5.03 -6.48 5.54 1.67 -9.93 -5.58 -8.49 7.22
Nucleolus 2.77 2.85 2.65 -2.01 -4.25 -2.84 -2.13 -2.59 1.06 -1.76 1.40 2.61 2.03 1.15 -2.30
Nucleus 10.48 0.23 -1.14 -17.80 -1.22 -14.65 -18.22 -17.34 -2.99 5.75 -7.57 -15.37 -14.86 -6.00 5.81
Peroxisomes 3.55 6.21 5.49 2.14 -2.01 -2.96 1.63 6.29 3.94 0.49 -0.99 5.27 6.81 4.12 -0.28
Vacuole 4.94 -5.09 -26.18 -29.29 -9.99 -31.62* -15.82 -19.90 9.76 5.24 -26.91 -16.17 -32.25 -5.29 -1.88
Vacuole Periphery 1.64 5.63 0.65 -0.63 -2.86 -1.53 6.93 1.20 2.21 -2.43 0.12 8.73 0.96 0.86 -3.71