Standard name
Description Putative divalent metal ion transporter involved in iron homeostasis; transcriptionally regulated by metal ions; member of the Nramp family of metal transport proteins; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.2 0.14 0.06 0 0 0 0 0 0 0 0 0
Cytoplasm 0.57 0.68 0.63 0.92 0.73 0.69 0.64 0.75 0.81 0.76 0.82 0.88 0.17 0.4 0.75 0.4 0.37 0.36 0 0 0 0.06 0.07 0.07
Endoplasmic Reticulum 0.09 0.27 0.21 0.05 0 0.05 0 0 0 0 0 0 0.73 0.5 0.2 0 0 0 0.45 0.44 0.39 0.49 0.45 0.49
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.07 0.06 0.08 0.09 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0.09 0.13 0.1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.27 0.16 0.2 0.05 0.41 0.36 0.49 0.29 0.31 0.36 0.24 0.16 0 0.09 0.05 0.63 0.67 0.65 0.45 0.46 0.5 0.38 0.41 0.33
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 23 9 1 0 0 0 0 0 2 1 2 2
Bud 0 0 0 0 0 0 1 1 0 0 1 0 1 1 0 0 0 0 0 1 0 2 1 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2 0 0 0 0 1 0 0 0 0 0 0 66 51 29 0 0 0 0 0 0 0 0 0
Cytoplasm 51 102 51 56 64 120 131 210 147 124 169 168 56 146 336 135 96 97 6 7 0 14 15 14
Endoplasmic Reticulum 8 40 17 3 1 8 5 3 3 2 1 4 243 183 92 13 9 2 61 81 43 118 103 101
Endosome 4 0 0 0 0 3 0 0 0 0 0 0 1 5 1 19 9 8 1 2 2 1 3 4
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 4 1 0 1 1 2 1 0 2 2 0 1
Mitochondria 4 0 3 0 2 2 14 16 14 14 8 14 0 0 0 5 1 3 0 0 1 5 3 4
Nucleus 1 0 1 0 0 2 10 14 7 4 2 0 1 0 1 31 34 27 0 1 0 0 0 0
Nuclear Periphery 0 1 0 0 0 0 1 1 1 2 2 0 3 0 0 0 0 1 1 0 0 0 0 0
Nucleolus 2 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0
Vac/Vac Membrane 24 24 16 3 36 62 101 82 56 59 50 30 5 34 21 213 176 176 61 84 55 91 93 67
Unique Cell Count 90 150 81 61 88 174 205 281 182 163 206 190 331 366 451 337 261 272 137 183 110 241 230 205
Labelled Cell Count 96 167 88 64 103 198 263 327 228 205 233 216 404 431 481 417 327 316 137 183 110 241 230 205


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.7 9.2 7.2 8.3 8.0 6.8 5.8 5.8 5.7 5.2 5.3 5.0 15.5 10.3 7.4 7.7 9.1 8.9 7.2 7.3 8.6
Std Deviation (1e-4) 1.5 1.8 1.6 1.9 1.9 1.9 1.7 1.6 1.8 1.8 1.4 1.3 4.5 2.4 1.7 1.8 1.9 2.1 1.6 1.7 1.7
Intensity Change (Log2) 0.2 0.16 -0.07 -0.31 -0.3 -0.33 -0.48 -0.44 -0.51 1.11 0.52 0.04 0.09 0.33 0.31 0.0 0.03 0.26


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 4.4 3.6 0 0 0 0
Cytoplasm 3.9 1.4 0.9 0.1 2.1 3.1 2.1 3.4 4.9 -8.5 -3.8 2.1 -3.7 -4.2 -4.4
Endoplasmic Reticulum -2.7 -4.2 -4.1 -5.3 0 -5.5 -5.4 -6.4 -5.3 8.8 4.8 -0.1 -5.4 -5.2 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 1.2 1.4 0 1.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 2.4 3.1 2.5
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -2.6 3.0 2.6 4.6 1.7 1.8 2.6 0.8 -0.8 0 -2.7 -4.9 7.1 7.6 7.1


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Smf3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available