Standard name
Human Ortholog
Description Protein involved in mismatch repair and meiotic recombination; only certain frameshift intermediates are mismatch repair substrates; forms a complex with Mlh1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.54 0.46 0.79 0.72 0.73 0.65 0.67 – 0.47 0.54 0.45 0.56 0.42 0.47 0.61 0.67 0.61 0.64 0.34 0.34 0.37 0.3 0.39 0.44
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0.05 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.28 0.19 0 0.07 0.05 0.26 0.27 – 0.55 0.45 0.56 0.34 0.25 0.07 0.06 0 0 0 0.08 0.05 0.07 0.06 0.09 0.09
Nucleus 0.09 0.15 0.11 0.14 0.11 0.1 0.11 – 0.12 0.09 0.08 0.09 0.27 0.2 0.14 0.08 0.09 0.05 0.21 0.25 0.14 0.14 0.09 0.05
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.27 0.31 0.11 0.09 0.12 0.09 0 – 0 0 0.05 0.07 0.19 0.35 0.25 0.3 0.34 0.34 0.28 0.3 0.33 0.43 0.32 0.3
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 2 0 2 0 0 – 0 0 0 0 0 0 0 0 0 0 2 0 2 0 1 4
Bud 1 0 0 0 1 4 3 – 14 10 17 3 2 0 1 0 0 0 1 0 3 4 6 12
Bud Neck 0 0 13 4 23 12 17 – 7 3 3 12 0 0 1 3 0 0 0 0 2 3 4 10
Bud Site 0 0 0 0 1 1 1 – 9 3 2 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 0 1 1 2 – 0 0 2 1 0 0 0 0 0 2 0 0 0 0 0 1
Cytoplasm 100 71 262 270 448 459 555 – 368 440 324 329 158 199 312 294 145 137 77 68 177 101 193 246
Endoplasmic Reticulum 1 2 2 2 0 0 2 – 0 0 0 0 1 0 1 32 11 16 0 0 0 2 6 6
Endosome 1 0 0 1 5 3 2 – 2 2 0 5 2 6 1 13 5 8 2 0 3 1 4 4
Golgi 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0 0 0 1 0 1 1 0 2 0
Mitochondria 53 30 3 25 31 180 222 – 427 368 401 204 92 31 28 12 5 6 17 10 34 19 46 51
Nucleus 16 24 37 54 65 69 88 – 97 75 61 54 100 83 72 35 21 11 48 51 67 45 44 25
Nuclear Periphery 0 0 0 0 0 0 1 – 2 1 0 0 0 0 1 0 1 0 0 0 0 0 0 0
Nucleolus 1 0 0 0 0 0 1 – 1 1 1 3 1 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 3 10 6 5 – 5 11 3 21 0 0 1 0 3 0 9 3 12 6 13 18
Vac/Vac Membrane 50 49 36 33 71 60 36 – 32 35 36 40 73 148 125 133 81 73 65 61 157 144 160 170
Unique Cell Count 186 156 330 374 611 703 825 779 814 718 592 375 425 509 440 239 215 231 204 479 336 496 565
Labelled Cell Count 223 176 357 392 659 795 935 964 949 850 673 429 467 543 522 272 255 231 204 479 336 496 565


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 3.9 4.9 4.3 5.0 3.8 3.9 – 3.4 3.5 3.3 3.9 3.7 4.0 3.9 5.6 6.0 5.6 3.6 3.7 4.1
Std Deviation (1e-4) 0.5 0.7 0.8 1.0 1.2 0.9 1.1 – 0.7 0.8 0.7 0.7 0.8 0.8 0.7 1.1 1.2 1.0 0.7 1.2 1.3
Intensity Change (Log2) – – – -0.17 0.02 -0.38 -0.31 – -0.54 -0.49 -0.57 -0.35 -0.39 -0.31 -0.34 0.19 0.28 0.2 -0.45 -0.41 -0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 6.4 6.7 6.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.1487 -0.5801 -0.6111 0.179 -0.6333 -0.4359 -0.4791 -0.9238 -0.6641 -0.6838 -1.1166 -0.7413 -0.3288 -0.63 -0.9922 -0.9841 -0.968 -0.802
Actin 0.0478 0.0011 0.0037 0.0181 0.0034 0.0061 0.0152 0.0003 0.015 0.0018 0.0004 0.0034 0.0662 0.0003 0.0039 0.0005 0.0004 0.0007
Bud 0.0009 0.0023 0.0013 0.0009 0.0004 0.0004 0.0008 0.0001 0.0008 0.0005 0.0001 0.0002 0.0005 0.0001 0.0002 0.0001 0.0001 0.0001
Bud Neck 0.0087 0.0006 0.0047 0.0026 0.0007 0.0046 0.008 0.0003 0.0008 0.0008 0.0014 0.0033 0.0027 0.0029 0.0009 0.0005 0.0012 0.004
Bud Periphery 0.0017 0.0006 0.0019 0.0022 0.0011 0.0004 0.0012 0.0001 0.0005 0.0005 0 0.0003 0.0008 0.0001 0.0002 0 0.0001 0.0001
Bud Site 0.0066 0.004 0.0065 0.0031 0.0006 0.0004 0.0109 0.0008 0.0015 0.0009 0.0001 0.0005 0.0018 0.0015 0.0009 0.0001 0.0005 0.0002
Cell Periphery 0.0006 0.0002 0.0004 0.001 0.0001 0.0002 0.0004 0.0001 0.0001 0.0001 0 0.0001 0.0003 0.0001 0.0001 0 0 0
Cytoplasm 0.1161 0.2083 0.1122 0.0525 0.1101 0.16 0.1058 0.1843 0.1574 0.135 0.1518 0.1936 0.1633 0.1638 0.1312 0.1905 0.1225 0.1812
Cytoplasmic Foci 0.0382 0.0136 0.016 0.0086 0.0492 0.0121 0.0599 0.0105 0.0234 0.0122 0.0558 0.0195 0.0269 0.004 0.0042 0.0033 0.0058 0.0071
Eisosomes 0.0009 0.0002 0.0002 0.0005 0.0002 0.0001 0.0002 0.0001 0.0004 0 0 0.0001 0.0004 0.0003 0.0002 0.0001 0 0
Endoplasmic Reticulum 0.0121 0.0091 0.004 0.0034 0.0069 0.0025 0.0072 0.0041 0.0063 0.0036 0.0019 0.0037 0.0085 0.0031 0.0038 0.0034 0.0013 0.0024
Endosome 0.0473 0.0112 0.014 0.0513 0.0837 0.0136 0.0575 0.0047 0.0206 0.0456 0.0799 0.018 0.0333 0.0025 0.0095 0.0033 0.0012 0.0056
Golgi 0.0199 0.0004 0.0049 0.0332 0.0164 0.0119 0.0219 0.0002 0.0052 0.006 0.0175 0.0048 0.0098 0.0001 0.0011 0.0001 0.0001 0.0003
Lipid Particles 0.0343 0.0026 0.0137 0.0185 0.0114 0.0073 0.0442 0.0008 0.0103 0.0079 0.0021 0.0055 0.0176 0.0005 0.0031 0.0002 0.0001 0.0017
Mitochondria 0.0268 0.0011 0.0035 0.1027 0.0069 0.0093 0.0301 0.0009 0.0189 0.0041 0.0021 0.011 0.0033 0.0008 0.0018 0.0006 0.0004 0.0009
None 0.3448 0.4716 0.4447 0.2678 0.2595 0.2299 0.2864 0.5384 0.2895 0.2624 0.4004 0.2089 0.2738 0.6152 0.2699 0.3285 0.4032 0.178
Nuclear Periphery 0.0462 0.0186 0.0186 0.0106 0.0875 0.0141 0.022 0.0174 0.0226 0.0222 0.0082 0.0146 0.0273 0.0107 0.0181 0.029 0.0061 0.0158
Nucleolus 0.005 0.0036 0.0104 0.0038 0.0044 0.0063 0.0039 0.0033 0.0037 0.0028 0.0025 0.0047 0.0029 0.0026 0.0113 0.0021 0.0018 0.0043
Nucleus 0.18 0.197 0.2789 0.3729 0.2416 0.4521 0.2632 0.2067 0.3499 0.4335 0.23 0.4509 0.2881 0.1738 0.4982 0.4055 0.4017 0.5615
Peroxisomes 0.0123 0.0005 0.0044 0.0043 0.0185 0.0014 0.0203 0.0005 0.0108 0.0178 0.0005 0.0026 0.0212 0.0001 0.0075 0.0001 0.0002 0.0003
Punctate Nuclear 0.0391 0.048 0.051 0.0082 0.0826 0.0634 0.0215 0.0234 0.0581 0.0297 0.0405 0.0502 0.0411 0.0149 0.0304 0.0307 0.0524 0.032
Vacuole 0.0075 0.0049 0.0039 0.0238 0.0055 0.0033 0.016 0.0026 0.0028 0.0103 0.0035 0.0032 0.0084 0.0021 0.0029 0.0011 0.0006 0.0028
Vacuole Periphery 0.0032 0.0006 0.001 0.0101 0.0093 0.0006 0.0034 0.0005 0.0011 0.0023 0.0012 0.0009 0.0017 0.0005 0.0008 0.0004 0.0002 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.0772 3.3068 4.9076 4.6764 4.111 4.7534 5.6123 4.8357 5.3008 6.49
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1314 1472 99 576 979 831 1034 184 2293 2303 1133 760

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 572.86 725.04 964.87 772.05 700.77 740.82 793.21 798.04 627.47 730.73 808.21 778.34
Standard Deviation 60.99 100.87 89.89 85.55 74.01 94.80 95.20 100.39 92.05 99.01 106.43 90.06
Intensity Change Log 2 — 0.339878 0.752152 0.430512 — 0.080182 0.178762 0.187520 — 0.202801 0.465054 0.301901

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000038 0.000307 0.000897 0.000179 0.000052 0.000262 0.000299 0.000099 0.000044 0.000291 0.000351 0.000159
Bud Neck 0.006337 0.014313 0.002424 0.002883 0.001864 0.008843 0.003368 0.001887 0.004427 0.012340 0.003286 0.002642
Bud Site 0.000717 0.004320 0.001493 0.004120 0.000924 0.004084 0.003266 0.003909 0.000805 0.004235 0.003111 0.004069
Cell Periphery 0.000155 0.000139 0.000244 0.000154 0.000136 0.000186 0.000292 0.000120 0.000147 0.000156 0.000288 0.000146
Cytoplasm 0.425763* 0.295647* 0.075896 0.192649* 0.320345* 0.284453* 0.158682* 0.171712* 0.380755* 0.291608* 0.151448* 0.187580*
Cytoplasmic Foci 0.134372 0.074968 0.011639 0.007332 0.074177 0.113563 0.006827 0.030099 0.108672 0.088894 0.007247 0.012844
Eisosomes 0.000116 0.000060 0.000106 0.000039 0.000064 0.000094 0.000042 0.000073 0.000094 0.000072 0.000048 0.000047
Endoplasmic Reticulum 0.000538 0.001237 0.012018 0.003368 0.000830 0.000850 0.003928 0.002079 0.000663 0.001097 0.004635 0.003056
Endosome 0.003692 0.004280 0.003705 0.001476 0.002027 0.006438 0.003356 0.005118 0.002981 0.005059 0.003387 0.002358
Golgi 0.000514 0.001171 0.000136 0.000051 0.000291 0.001782 0.001159 0.000155 0.000419 0.001391 0.001070 0.000076
Lipid Particles 0.010557 0.004648 0.004977 0.001537 0.004718 0.007364 0.001048 0.001313 0.008064 0.005628 0.001391 0.001483
Mitochondria 0.003612 0.007430 0.000628 0.000454 0.001838 0.009556 0.000988 0.000497 0.002855 0.008197 0.000957 0.000464
Mitotic Spindle 0.000286 0.001581 0.000316 0.000860 0.001653 0.005846 0.001819 0.002304 0.000869 0.003119 0.001688 0.001210
None 0.017539 0.007265 0.003189 0.005986 0.005832 0.007259 0.007809 0.004404 0.012541 0.007263 0.007406 0.005603
Nuclear Periphery 0.000574 0.001259 0.001910 0.001610 0.000922 0.001192 0.001512 0.001100 0.000723 0.001235 0.001546 0.001487
Nuclear Periphery Foci 0.000371 0.000385 0.002033 0.000959 0.000426 0.000328 0.000469 0.000437 0.000394 0.000364 0.000605 0.000833
Nucleolus 0.002912 0.003451 0.002156 0.001008 0.001579 0.003842 0.001088 0.000724 0.002343 0.003592 0.001182 0.000939
Nucleus 0.371693* 0.549945* 0.831386* 0.749642* 0.568453* 0.512455* 0.780828* 0.720059* 0.455700* 0.536417* 0.785246* 0.742480*
Peroxisomes 0.002899 0.007587 0.000656 0.000229 0.001819 0.006754 0.000246 0.000309 0.002438 0.007286 0.000282 0.000249
Vacuole 0.016824 0.018875 0.043903 0.025285 0.011820 0.023272 0.021421 0.052783 0.014687 0.020462 0.023385 0.031943
Vacuole Periphery 0.000488 0.001133 0.000287 0.000179 0.000230 0.001577 0.001553 0.000820 0.000378 0.001293 0.001442 0.000334

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.69 -6.69 -9.96 0.95 5.44 -1.87 -3.72 -4.87 -0.41 1.40 -4.06 -4.76 -10.73 0.57 2.59
Bud Neck -4.88 2.61 2.74 6.68 -0.01 -6.05 -4.92 -2.72 4.56 1.96 -7.09 -0.28 1.33 7.75 1.58
Bud Site -4.30 -3.85 -2.83 0.26 -1.13 -3.73 -3.57 -1.54 -0.89 -0.93 -5.49 -4.49 -2.89 -0.50 -0.83
Cell Periphery 1.19 -3.87 0.43 -0.50 3.89 -2.43 -1.52 0.21 2.16 1.54 -0.83 -1.58 0.22 0.81 1.60
Cytoplasm 13.56 22.53 21.04 9.57 -6.66 3.02 17.69 8.22 6.23 -1.58 11.88 30.93 20.73 11.40 -4.94
Cytoplasmic Foci 10.08 21.35 28.15 18.64 1.26 -6.02 18.54 7.73 11.61 -2.56 4.39 32.80 28.71 23.17 -2.51
Eisosomes 8.25 -1.30 10.36 3.38 5.49 -5.30 3.08 -2.96 0.32 -4.38 4.39 7.45 7.27 3.77 0.26
Endoplasmic Reticulum -6.11 -7.70 -11.58 -9.65 5.35 -0.09 -13.78 -6.48 -6.46 0.80 -5.25 -15.92 -13.16 -11.78 3.60
Endosome -1.19 -1.62 3.76 5.60 3.65 -5.85 -1.13 -0.95 1.22 -0.48 -4.65 -0.20 1.16 4.56 0.97
Golgi -2.05 4.21 6.51 3.65 3.70 -2.43 -1.09 1.55 2.76 1.49 -3.23 -0.76 5.88 4.53 1.63
Lipid Particles 4.79 2.97 7.70 8.34 3.85 -2.86 6.28 5.07 7.78 -2.32 3.06 9.03 9.02 11.36 -0.15
Mitochondria -2.84 3.95 4.26 6.37 2.86 -3.57 0.94 1.58 4.52 2.47 -4.63 3.27 4.31 7.70 3.30
Mitotic Spindle -3.23 -0.99 -2.09 -0.11 -0.21 -1.87 -1.44 -1.16 -0.41 -0.82 -2.73 -2.56 -1.70 -0.12 -0.06
None 5.48 5.75 6.08 1.36 -0.88 -0.87 -0.65 1.00 1.58 1.61 4.24 4.89 5.54 1.82 0.90
Nuclear Periphery -6.91 -7.41 -9.84 -5.95 -0.02 -1.66 -9.29 -4.98 -3.58 0.31 -5.77 -14.75 -10.44 -6.88 -1.09
Nuclear Periphery Foci -0.85 -4.24 -2.21 -2.15 0.64 1.48 -2.87 -2.24 -2.61 -0.40 0.07 -4.38 -2.39 -2.40 -1.34
Nucleolus -1.07 1.07 4.32 6.22 2.52 -3.45 1.47 3.54 5.72 2.47 -2.95 3.43 5.11 8.21 1.64
Nucleus -16.27 -15.98 -26.32 -12.54 2.42 4.15 -16.95 -2.59 -4.77 5.56 -8.83 -32.88 -20.40 -13.58 6.05
Peroxisomes -4.75 5.90 7.48 7.93 4.45 -5.87 4.34 4.25 8.68 -0.38 -6.88 8.31 8.56 10.75 0.95
Vacuole -1.96 -4.50 -7.35 -6.64 1.28 -5.78 -9.04 -7.53 -6.67 -5.47 -5.32 -9.42 -10.46 -9.11 -4.36
Vacuole Periphery -1.58 1.60 4.07 2.41 3.74 -2.66 -1.26 -0.78 1.63 0.72 -2.88 -1.04 1.16 3.16 1.23
Standard name
Human Ortholog
Description Protein involved in mismatch repair and meiotic recombination; only certain frameshift intermediates are mismatch repair substrates; forms a complex with Mlh1p
Localization
Cell Percentages cytoplasm (14%), nucleus (8%), mixed (62%)
Cell Cycle Regulation Yes
cytoplasm - MA (3.5e-06)
Subcompartmental Group N/A

Mlh2

Mlh2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mlh2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available