Standard name
Human Ortholog
Description Cytoplasmic thioredoxin isoenzyme; part of thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p; required for ER-Golgi transport and vacuole inheritance; with Trx2p, facilitates mitochondrial import of small Tims Tim9p, Tim10p, Tim13p by maintaining them in reduced form; abundance increases iunder DNA replication stress; TRX1 has a paralog, TRX2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.99 0.95 0.98 0.95 0.89 0.89 – 0.8 0.79 0.83 0.99 0.98 0.99 0.96 0.99 0.99 0.96 0.89 0.97 0.9 0.88 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 – 0.06 0.09 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.08 0 0 0.08 0.05 – 0.1 0.08 0.05 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 2 1 1 – 2 2 4 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 0 1 – 1 0 1 0 0 0 3 0 1 4 2 0 0 4 5
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 0 4 3 4 1 – 10 8 6 0 1 2 5 2 7 0 0 0 0 0 0
Cytoplasm 633 210 163 63 236 243 229 247 – 170 166 228 282 258 341 340 275 331 608 188 147 184 254 233
Endoplasmic Reticulum 3 2 0 1 2 6 8 12 – 6 5 9 1 3 3 7 18 8 3 1 0 1 7 4
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 1 1 0 0 0 1
Golgi 0 0 0 0 0 0 0 0 – 1 0 1 0 0 0 1 0 0 0 0 0 0 0 0
Mitochondria 1 2 0 0 0 2 7 6 – 12 19 22 0 0 0 0 2 0 1 0 0 0 1 2
Nucleus 0 0 0 0 2 2 0 3 – 3 5 8 0 0 0 0 0 0 7 8 2 6 8 7
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 – 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 9 2 1 5 4 3 21 15 – 21 16 13 1 4 4 12 1 2 4 2 0 7 9 10
Unique Cell Count 645 215 164 66 240 257 257 277 212 209 276 284 262 345 354 279 334 637 211 153 204 290 270
Labelled Cell Count 646 216 165 69 248 262 270 286 227 221 293 284 266 350 368 298 350 637 211 153 204 290 270


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 37.1 35.4 27.4 27.1 25.2 22.7 20.9 20.9 – 19.9 19.5 20.9 35.5 36.6 38.3 55.3 54.5 58.8 37.8 37.8 39.7
Std Deviation (1e-4) 7.0 9.2 4.6 5.8 5.4 5.4 5.5 5.0 – 5.2 5.3 6.2 6.5 8.2 8.3 14.2 14.3 13.9 7.9 9.0 10.6
Intensity Change (Log2) – – – -0.01 -0.12 -0.27 -0.39 -0.39 – -0.46 -0.49 -0.39 0.37 0.42 0.48 1.02 0.99 1.1 0.47 0.47 0.53


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm -2.1 -0.9 -2.6 -4.1 -4.1 – -5.8 -5.9 -5.4 -0.1 -0.9 -0.6 -2.1 -0.8 -0.3
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 0 3.3 0
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 – 3.1 4.0 3.7 0 0 0 0 0 0
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 3.4 2.6 – 3.8 3.2 2.4 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 31.5055 34.8439 32.3748 30.9515 29.301 31.8934 37.1274 38.6876 36.3438 34.0958 34.5141 35.8121 28.879 35.8515 33.9782 30.0665 27.2447 31.6923
Actin 0.0005 0.0001 0.0039 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0188 0.002 0.0002 0.0065 0.0007 0.0632
Bud 0.0006 0.0001 0.0005 0 0.0009 0.0001 0 0 0 0 0 0.0001 0.0004 0.0003 0 0.0049 0.0001 0.0003
Bud Neck 0.0003 0.0002 0.0003 0.0003 0.0006 0.0007 0.0001 0.0001 0.0002 0.0003 0.0003 0.0006 0.0005 0.0001 0.0002 0.001 0.0005 0.0011
Bud Periphery 0.0005 0 0.0001 0 0.0007 0 0 0 0 0 0 0 0.0002 0.0002 0 0.0058 0 0.0006
Bud Site 0.0003 0 0.0001 0 0.0002 0 0 0 0 0 0 0 0.0023 0.0002 0 0.0205 0.0001 0.0003
Cell Periphery 0.0002 0 0 0 0.0003 0 0 0 0 0 0 0 0.0001 0 0 0.0008 0 0.0002
Cytoplasm 0.8074 0.9241 0.8502 0.8922 0.8835 0.8362 0.8986 0.956 0.9274 0.9532 0.9292 0.9016 0.8272 0.9769 0.9729 0.8802 0.8954 0.8657
Cytoplasmic Foci 0.0031 0.0002 0.0005 0.0003 0.0009 0.0002 0.0001 0.0001 0.0002 0.0002 0.0002 0.0003 0.0044 0.0002 0.0003 0.0029 0.0077 0.0012
Eisosomes 0 0 0 0 0 0 0 0 0 0 0 0 0.0001 0 0 0 0 0.0007
Endoplasmic Reticulum 0.0019 0.0005 0.0006 0.0006 0.001 0.0017 0.0006 0.0004 0.0004 0.0003 0.0008 0.0008 0.0165 0.0002 0.0002 0.0029 0.0005 0.0075
Endosome 0.0024 0.0001 0.0005 0.0001 0.0005 0.0006 0.0001 0 0.0001 0 0.0001 0.0001 0.0169 0.0001 0 0.0122 0.0517 0.0024
Golgi 0.0002 0 0.0003 0 0 0 0 0 0 0 0 0 0.0017 0 0 0.0016 0.0261 0.0017
Lipid Particles 0.001 0 0.0002 0 0.0001 0 0 0 0 0 0 0 0.0011 0 0 0.0004 0.0004 0.0006
Mitochondria 0.0017 0.0001 0.0003 0.0001 0.0006 0.0001 0.0001 0.0001 0 0 0 0.0001 0.0024 0.0033 0.0001 0.0031 0.0014 0.0118
None 0.0018 0.0002 0.0004 0.0003 0.0004 0.0002 0.0002 0.0001 0.0001 0.0002 0.0002 0.0002 0.0076 0.0003 0.0002 0.0006 0.0003 0.0012
Nuclear Periphery 0.0155 0.0049 0.011 0.0074 0.0114 0.015 0.0076 0.0029 0.0046 0.0041 0.0101 0.007 0.0303 0.0013 0.0014 0.0052 0.0012 0.0046
Nucleolus 0.0052 0 0.0002 0 0.001 0 0 0 0 0 0 0 0.0003 0.0001 0 0.0004 0 0.0001
Nucleus 0.1474 0.069 0.1289 0.097 0.0905 0.1431 0.0916 0.0396 0.0664 0.041 0.0581 0.0882 0.0603 0.0144 0.0242 0.0344 0.0117 0.0352
Peroxisomes 0.0009 0 0.0001 0 0.0001 0 0 0 0 0 0 0 0.0006 0.0001 0 0.0002 0.0004 0.0002
Punctate Nuclear 0.0051 0.0002 0.0009 0.001 0.0009 0.0006 0.0004 0.0002 0.0002 0.0004 0.0005 0.0005 0.005 0.0001 0.0002 0.0004 0.0003 0.0007
Vacuole 0.0029 0.0003 0.0005 0.0004 0.0054 0.0012 0.0002 0.0001 0.0002 0.0001 0.0002 0.0002 0.002 0.0001 0.0001 0.0128 0.0005 0.0002
Vacuole Periphery 0.0009 0 0.0003 0.0001 0.0008 0.0003 0.0001 0 0 0 0.0001 0.0001 0.0011 0.0001 0 0.0029 0.0008 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 438.5325 354.0838 412.8857 390.8055 421.8472 413.5476 476.2861 460.617 466.7994 426.4311
Translational Efficiency 1.9676 2.4318 1.9424 2.0456 1.9395 2.2058 2.0845 2.2102 2.2717 2.215

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1505 1795 242 1379 1660 2007 181 1594 3165 3802 423 2973

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1883.72 2338.63 4326.09 3743.27 2030.01 2251.34 4350.77 3583.08 1960.45 2292.55 4336.65 3657.38
Standard Deviation 370.33 475.78 729.90 828.97 403.00 472.62 619.10 948.45 394.63 476.12 684.79 898.58
Intensity Change Log 2 — 0.312079 1.199479 0.990715 — 0.149297 1.099784 0.819713 — 0.229940 1.148629 0.904550

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000262 0.000151 0.000088 0.001225 0.000250 0.000101 0.000195 0.002077 0.000255 0.000125 0.000134 0.001682
Bud Neck 0.000581 0.000512 0.001044 0.003507 0.000500 0.000456 0.002797 0.004704 0.000538 0.000482 0.001794 0.004149
Bud Site 0.001097 0.001206 0.000300 0.009008 0.001342 0.000633 0.001760 0.015010 0.001226 0.000904 0.000924 0.012226
Cell Periphery 0.000137 0.000115 0.000513 0.000495 0.000133 0.000071 0.001199 0.000273 0.000135 0.000091 0.000806 0.000376
Cytoplasm 0.607463* 0.531201* 0.261974* 0.483232* 0.596616* 0.581917* 0.312045* 0.572453* 0.601774* 0.557973* 0.283399* 0.531069*
Cytoplasmic Foci 0.003632 0.002101 0.007855 0.005539 0.002947 0.001878 0.025111 0.011301 0.003273 0.001983 0.015239 0.008628
Eisosomes 0.000001 0.000001 0.000002 0.000007 0.000001 0.000001 0.000006 0.000008 0.000001 0.000001 0.000004 0.000008
Endoplasmic Reticulum 0.000239 0.000089 0.000026 0.004073 0.000220 0.000086 0.000057 0.000321 0.000229 0.000088 0.000039 0.002061
Endosome 0.000144 0.000105 0.000014 0.002376 0.000118 0.000057 0.000059 0.008496 0.000131 0.000079 0.000033 0.005657
Golgi 0.000317 0.000416 0.000145 0.002685 0.000689 0.000116 0.000247 0.015838 0.000513 0.000258 0.000189 0.009737
Lipid Particles 0.000170 0.000107 0.000188 0.001099 0.000165 0.000079 0.001173 0.001853 0.000167 0.000092 0.000610 0.001503
Mitochondria 0.000171 0.000198 0.000298 0.000463 0.000169 0.000135 0.000322 0.002204 0.000170 0.000165 0.000308 0.001396
Mitotic Spindle 0.001603 0.000869 0.000149 0.027565 0.000990 0.001081 0.001215 0.027411 0.001281 0.000981 0.000605 0.027482
None 0.013105 0.019384 0.008958 0.014049 0.013108 0.019903 0.008916 0.016765 0.013107 0.019658 0.008940 0.015505
Nuclear Periphery 0.000093 0.000065 0.000385 0.000419 0.000094 0.000088 0.000765 0.000480 0.000093 0.000077 0.000548 0.000451
Nuclear Periphery Foci 0.001528 0.000399 0.000235 0.001900 0.001301 0.000620 0.000882 0.002241 0.001409 0.000516 0.000512 0.002083
Nucleolus 0.000185 0.000121 0.000054 0.000191 0.000090 0.000225 0.000098 0.000198 0.000135 0.000176 0.000073 0.000195
Nucleus 0.365880* 0.440938* 0.714041* 0.428893* 0.378187* 0.390263* 0.620465* 0.299794* 0.372335* 0.414188* 0.674000* 0.359675*
Peroxisomes 0.000032 0.000008 0.000004 0.000087 0.000025 0.000011 0.000011 0.001085 0.000028 0.000009 0.000007 0.000622
Vacuole 0.003268 0.001928 0.003688 0.011204 0.002841 0.002220 0.022612 0.012828 0.003044 0.002082 0.011785 0.012075
Vacuole Periphery 0.000091 0.000087 0.000040 0.001983 0.000214 0.000059 0.000067 0.004660 0.000156 0.000072 0.000052 0.003418

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 3.20 2.03 -0.86 -1.94 -1.53 2.79 -2.33 -1.05 -1.80 -0.33 4.09 -0.69 -1.31 -2.45 -1.12
Bud Neck 0.40 -7.24 -9.70 -9.80 1.43 0.36 -9.46 -8.03 -7.97 6.15 0.54 -11.61 -12.25 -12.24 5.61
Bud Site -0.41 0.14 -5.56 -4.84 -5.66 1.25 -5.45 -4.71 -6.19 -0.91 0.83 -4.30 -6.88 -7.83 -4.41
Cell Periphery 1.05 -7.82 -8.33 -8.66 5.22 4.77 -10.26 -8.49 -11.77 9.22 3.87 -12.62 -11.58 -13.42 10.22
Cytoplasm 10.45 34.42 13.32 4.14 -24.30 3.70 22.23 1.69 -1.83 -21.08 9.83 39.73 10.24 1.33 -32.64
Cytoplasmic Foci 2.05 -6.85 -7.72 -10.15 4.79 2.88 -9.19 -11.17 -12.79 7.74 3.28 -11.17 -13.34 -16.28 8.78
Eisosomes 1.89 -6.73 -6.45 -7.08 1.84 1.24 -8.94 -7.19 -8.61 6.76 2.15 -10.94 -9.54 -10.80 6.33
Endoplasmic Reticulum 3.65 5.80 -0.65 -0.96 -1.04 2.27 2.16 2.66 1.38 1.65 3.99 4.92 -0.32 -0.90 -0.99
Endosome 0.75 2.51 -1.41 -1.67 -2.07 1.46 -0.66 -3.17 -3.33 -3.11 1.38 1.66 -3.46 -3.72 -3.72
Golgi -0.18 -0.35 -0.83 -0.38 -0.74 1.23 0.35 -1.87 -2.75 -2.29 0.98 0.37 -2.01 -2.71 -2.43
Lipid Particles 1.23 -4.67 -9.78 -10.03 -2.95 2.76 -8.37 -7.26 -7.91 5.07 2.76 -9.14 -11.76 -12.45 2.53
Mitochondria -0.78 -8.86 -3.05 -1.56 5.37 2.02 -7.12 -2.16 -2.39 -0.10 0.31 -11.32 -2.64 -2.68 1.32
Mitotic Spindle 0.48 2.07 -3.38 -3.65 -4.31 -1.16 -0.78 -3.60 -2.85 -3.43 -0.62 0.99 -4.93 -4.60 -5.60
None -5.09 4.65 1.88 7.46 -3.70 -6.12 4.45 0.76 6.95 -3.98 -7.92 6.44 1.85 10.15 -5.45
Nuclear Periphery 4.32 -8.99 -14.56 -15.71 4.69 -2.72 -12.40 -10.37 -9.70 9.52 0.68 -14.74 -17.26 -17.46 9.82
Nuclear Periphery Foci 4.15 3.38 1.54 -4.24 -2.86 3.86 -2.37 1.65 -1.50 3.52 5.59 1.44 2.23 -3.68 0.94
Nucleolus 0.57 0.76 0.27 -0.62 -2.51 -1.18 -3.88 -1.82 0.73 2.65 -0.47 -0.27 -0.28 0.37 -0.03
Nucleus -10.16 -28.40 -2.20 7.34 26.35 -3.05 -7.66 9.84 13.18 11.90 -9.14 -22.57 5.41 14.51 25.12
Peroxisomes 1.88 1.56 0.17 -3.04 -2.42 1.16 -0.61 -1.30 -1.41 -1.24 2.13 0.84 -1.27 -1.55 -1.39
Vacuole 3.02 -5.17 -19.66 -20.30 -10.79 -1.03 -9.96 -20.79 -20.33 3.70 0.98 -10.38 -28.53 -28.71 -2.34
Vacuole Periphery 0.27 -0.23 -1.13 -1.17 -1.11 1.26 0.56 -1.57 -2.16 -1.89 1.29 0.59 -1.95 -2.43 -2.21
Standard name
Human Ortholog
Description Cytoplasmic thioredoxin isoenzyme; part of thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p; required for ER-Golgi transport and vacuole inheritance; with Trx2p, facilitates mitochondrial import of small Tims Tim9p, Tim10p, Tim13p by maintaining them in reduced form; abundance increases iunder DNA replication stress; TRX1 has a paralog, TRX2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (85%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Trx1

Trx1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Trx1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available