Standard name
Human Ortholog
Description Putative integral membrane protein of unknown function; interacts with Ulp1p at the nuclear periphery; member of DUP240 gene family
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud – 0 0 0 0 0 0 0 – 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0.95 0.9 0.94 0.93 0.89 0.86 0.86 – 0.87 0.84 0.83 0.95 0.91 0.88 0.64 0.65 0.71 0 0.4 0.37 0.46 0.47 0.35
Endoplasmic Reticulum – 0 0 0 0 0.06 0 0 – 0 0 0.05 0 0.08 0.1 0 0 0 0.23 0 0.07 0 0 0.07
Endosome – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0.05 0 0.11 0 0.05 0 0 0
Golgi – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0
Mitochondria – 0.05 0.16 0 0.05 0.06 0.11 0.06 – 0.08 0.16 0.13 0 0 0 0 0 0 0.07 0.05 0.1 0.05 0 0.08
Nucleus – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery – 0.05 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0 0.05 0.05 0 0.05 0.07 0.06 – 0.09 0.05 0 0 0 0 0.39 0.44 0.35 0.38 0.42 0.37 0.36 0.4 0.39
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 2 1 1 2 – 2 1 2 2 5 4 2 2 1 0 0 1 1 2 6
Bud – 0 0 0 1 6 12 17 – 9 17 19 1 2 2 1 1 0 0 0 0 0 4 11
Bud Neck – 0 0 0 0 0 0 1 – 0 0 0 3 5 1 0 0 0 0 0 1 1 0 3
Bud Site – 0 0 0 0 0 0 2 – 0 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 1 1 3 2 – 1 4 0 4 6 4 1 1 0 0 0 0 0 0 1
Cytoplasm – 20 96 144 281 273 423 422 – 353 249 396 172 267 349 209 171 175 0 25 44 36 129 134
Endoplasmic Reticulum – 0 1 0 6 17 14 10 – 2 6 23 6 23 39 6 8 6 4 1 8 2 5 28
Endosome – 0 0 3 2 0 18 12 – 6 6 1 0 0 2 18 13 3 2 2 5 0 4 7
Golgi – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 1 0 0 0 0 3
Mitochondria – 1 17 6 14 19 54 31 – 32 47 64 0 0 0 10 7 1 1 3 11 3 11 32
Nucleus – 0 0 0 0 2 0 2 – 4 2 2 1 0 0 14 3 6 0 0 0 0 0 0
Nuclear Periphery – 1 0 2 0 0 0 1 – 1 1 1 0 0 0 0 0 1 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 1 – 0 1 0 0 1 0 1 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 1 3 – 1 4 0 0 0 0 1 0 0 0 0 0 0 3 3
Vac/Vac Membrane – 0 5 7 9 15 33 30 – 37 15 19 2 1 5 127 116 87 7 26 43 28 109 151
Unique Cell Count 21 107 154 301 308 493 490 407 298 475 182 293 397 326 263 246 20 63 120 79 276 390
Labelled Cell Count 22 119 162 316 334 560 536 448 354 527 191 310 406 390 322 281 20 63 120 79 276 390


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 4.1 3.8 3.9 4.6 4.7 4.4 5.2 – 5.1 5.1 5.1 5.1 5.0 5.7 7.5 7.3 7.6 5.4 5.7 6.2
Std Deviation (1e-4) – 0.8 0.8 1.0 1.8 1.5 1.6 4.8 – 1.7 1.8 1.7 1.0 1.0 1.2 1.8 1.7 1.9 1.4 1.6 1.6
Intensity Change (Log2) – – – 0.01 0.26 0.3 0.21 0.45 – 0.42 0.42 0.43 0.42 0.4 0.59 0.98 0.94 1.0 0.5 0.58 0.69


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 6.7 7.3 6.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.5754 1.0485 0.6735 0.7753 0.0761 0.9361 0.7008 1.6605 1.4076 1.3819 0.6705 1.4166 -0.9349 -0.3722 -0.6018 -0.8176 -0.5916 -0.7984
Actin 0.0295 0.0006 0.0102 0.0371 0.0153 0.0037 0.0198 0.0002 0.0045 0.0295 0.0002 0.0011 0.019 0.0005 0.0139 0.0001 0.0001 0.0002
Bud 0.0009 0.001 0.0046 0.0007 0.0004 0.0011 0.0007 0.0009 0.0009 0.0004 0.0005 0.0008 0.0002 0.0001 0.0003 0.0002 0.0001 0.0001
Bud Neck 0.0023 0.0003 0.0032 0.0008 0.0005 0.0022 0.0005 0.0003 0.0011 0.0003 0.0005 0.0013 0.0006 0.0001 0.0006 0.0005 0.0002 0.0005
Bud Periphery 0.005 0.0011 0.0034 0.001 0.0007 0.0041 0.0022 0.0016 0.0016 0.0009 0.001 0.0011 0.0004 0.0001 0.0006 0.0002 0.0001 0.0001
Bud Site 0.0023 0.0065 0.0119 0.0021 0.0007 0.0006 0.0016 0.005 0.0138 0.0007 0.0005 0.0004 0.0018 0.0008 0.0032 0.0003 0.0002 0.0001
Cell Periphery 0.0005 0.0004 0.0006 0.0002 0.0001 0.0003 0.0008 0.0011 0.0009 0.0004 0.0003 0.0003 0.0004 0.0003 0.0009 0.0001 0.0001 0.0001
Cytoplasm 0.1565 0.2846 0.2249 0.1749 0.2451 0.3462 0.1918 0.3203 0.2163 0.2033 0.1516 0.309 0.2479 0.3249 0.2929 0.3172 0.2696 0.3606
Cytoplasmic Foci 0.0566 0.0534 0.0532 0.0558 0.0548 0.068 0.0502 0.0443 0.0494 0.0613 0.0979 0.0549 0.0431 0.0501 0.0501 0.067 0.0598 0.0414
Eisosomes 0.0005 0.0001 0.0003 0.0003 0.0005 0.0001 0.0007 0.0002 0.0007 0.0002 0.0001 0.0001 0.0006 0.0001 0.0005 0 0 0
Endoplasmic Reticulum 0.0552 0.0337 0.035 0.0377 0.0142 0.0255 0.0395 0.0354 0.0573 0.0225 0.0126 0.0464 0.0944 0.0487 0.064 0.0295 0.0266 0.0422
Endosome 0.1285 0.1557 0.1224 0.2459 0.1335 0.1246 0.1255 0.1063 0.1588 0.1771 0.3247 0.1664 0.1327 0.1278 0.128 0.2725 0.3609 0.1399
Golgi 0.0112 0.005 0.0083 0.0174 0.0081 0.008 0.0069 0.0028 0.0066 0.0039 0.0047 0.0032 0.006 0.003 0.0051 0.0042 0.0054 0.0041
Lipid Particles 0.0142 0.006 0.0104 0.0176 0.0235 0.0137 0.0155 0.0056 0.0075 0.0091 0.0135 0.0056 0.0216 0.0063 0.0194 0.0051 0.0046 0.004
Mitochondria 0.0056 0.0048 0.0066 0.0053 0.0317 0.0111 0.0112 0.0039 0.0054 0.0028 0.0053 0.0047 0.0065 0.0008 0.0031 0.0035 0.0008 0.0037
None 0.4662 0.3655 0.4478 0.3129 0.4042 0.3435 0.4573 0.4122 0.4125 0.4419 0.2484 0.3275 0.3596 0.3868 0.3628 0.1932 0.1689 0.3231
Nuclear Periphery 0.0162 0.0093 0.0079 0.0143 0.01 0.0052 0.0092 0.0064 0.009 0.0047 0.009 0.005 0.0175 0.0103 0.0112 0.0119 0.0155 0.0066
Nucleolus 0.0008 0.0006 0.0008 0.0005 0.0006 0.0007 0.0009 0.0009 0.0014 0.0005 0.001 0.0006 0.0006 0.0003 0.0007 0.0003 0.0002 0.0002
Nucleus 0.0052 0.0041 0.0073 0.0033 0.0034 0.0029 0.0027 0.0036 0.006 0.0028 0.0036 0.0027 0.0031 0.0021 0.0066 0.004 0.0044 0.0029
Peroxisomes 0.0121 0.0006 0.008 0.009 0.0079 0.0039 0.0064 0.0006 0.0048 0.0023 0.0015 0.0007 0.0054 0.0007 0.0109 0.0004 0.0003 0.0003
Punctate Nuclear 0.005 0.0021 0.0031 0.0021 0.0118 0.0057 0.0038 0.001 0.0019 0.0017 0.0016 0.0016 0.004 0.0013 0.0032 0.0016 0.0009 0.0008
Vacuole 0.0221 0.0585 0.025 0.0497 0.0269 0.0247 0.0471 0.0416 0.0349 0.0301 0.1075 0.0593 0.03 0.0322 0.0179 0.0768 0.0742 0.0629
Vacuole Periphery 0.0037 0.0059 0.0051 0.0115 0.0061 0.0045 0.0057 0.0058 0.0047 0.0036 0.0141 0.0074 0.0048 0.0027 0.004 0.0115 0.0073 0.0062

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 24.1565 12.5387 20.6761 39.0644 29.2993 20.249 29.2295 35.1775 47.3948 39.4397
Translational Efficiency 1.5845 2.1379 1.6231 1.2066 1.2885 2.9185 1.3043 1.1982 1.2037 1.3296

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1386 1608 1203 1189 1763 667 2275 1156 3149 2275 3478 2345

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 714.77 656.26 908.69 987.06 659.67 727.58 823.48 962.68 683.92 677.17 852.95 975.04
Standard Deviation 96.49 116.18 135.90 241.47 87.29 98.66 156.89 242.62 95.46 115.97 155.34 242.34
Intensity Change Log 2 — -0.123212 0.346309 0.465659 — 0.141361 0.319989 0.545312 — 0.009833 0.333737 0.504438

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000328 0.000305 0.000982 0.018566 0.000252 0.000588 0.002871 0.004330 0.000285 0.000388 0.002217 0.011548
Bud Neck 0.015951 0.028986 0.008065 0.016822 0.016835 0.043656 0.024352 0.009863 0.016446 0.033287 0.018718 0.013391
Bud Site 0.004867 0.011458 0.008647 0.077904 0.006783 0.018639 0.040537 0.042258 0.005940 0.013563 0.029506 0.060332
Cell Periphery 0.000207 0.000127 0.000226 0.002970 0.000136 0.000178 0.000601 0.000871 0.000167 0.000142 0.000471 0.001935
Cytoplasm 0.367027* 0.385698* 0.231420* 0.212786* 0.405130* 0.229957* 0.385249* 0.235991* 0.388359* 0.340037* 0.332041* 0.224225*
Cytoplasmic Foci 0.210695* 0.232499* 0.083230 0.110124 0.242359* 0.307310* 0.088626 0.141896 0.228422* 0.254432* 0.086760 0.125786
Eisosomes 0.000197 0.000133 0.000079 0.000326 0.000199 0.000255 0.000106 0.000135 0.000198 0.000169 0.000097 0.000232
Endoplasmic Reticulum 0.019776 0.004118 0.100345* 0.072462 0.008713 0.006525 0.020581 0.025409 0.013582 0.004824 0.048171 0.049267
Endosome 0.061389 0.046967 0.081317 0.102251 0.039571 0.093638 0.050257 0.138468 0.049174 0.060650 0.061000 0.120105
Golgi 0.004109 0.005313 0.003654 0.033535 0.004847 0.016226 0.006689 0.035828 0.004522 0.008513 0.005640 0.034665
Lipid Particles 0.021878 0.015183 0.046658 0.069058 0.020168 0.016205 0.024232 0.038132 0.020920 0.015482 0.031989 0.053812
Mitochondria 0.003635 0.010260 0.001393 0.002299 0.004033 0.022543 0.002743 0.002440 0.003858 0.013861 0.002276 0.002368
Mitotic Spindle 0.001207 0.002280 0.009586 0.014358 0.001724 0.006457 0.017917 0.017570 0.001497 0.003505 0.015035 0.015941
None 0.001628 0.005139 0.001716 0.000458 0.002056 0.000716 0.001659 0.001474 0.001868 0.003842 0.001679 0.000959
Nuclear Periphery 0.001170 0.001187 0.012655 0.000632 0.001090 0.001159 0.002321 0.000792 0.001125 0.001179 0.005895 0.000711
Nuclear Periphery Foci 0.001520 0.001378 0.037050 0.008977 0.001127 0.000444 0.005692 0.006076 0.001300 0.001104 0.016539 0.007547
Nucleolus 0.000918 0.004867 0.001460 0.000227 0.001432 0.000907 0.000777 0.000476 0.001206 0.003706 0.001013 0.000350
Nucleus 0.072883 0.088237 0.107478 0.025968 0.106942 0.045729 0.114482 0.031770 0.091951 0.075774 0.112060 0.028828
Peroxisomes 0.004354 0.009691 0.002508 0.009602 0.004381 0.026907 0.005389 0.008139 0.004369 0.014739 0.004393 0.008881
Vacuole 0.202220* 0.142109 0.257745* 0.215824* 0.129776 0.154368 0.202662* 0.253340* 0.161661 0.145704 0.221715* 0.234318*
Vacuole Periphery 0.004038 0.004064 0.003785 0.004852 0.002447 0.007593 0.002256 0.004742 0.003147 0.005099 0.002785 0.004798

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.04 -1.87 -8.75 -8.78 -8.25 -1.64 -5.11 -5.60 -4.84 -1.45 -1.17 -5.38 -10.06 -9.94 -7.69
Bud Neck -6.03 4.97 0.22 6.60 -5.39 -7.14 -3.97 4.96 9.34 9.51 -9.19 -1.68 3.34 11.93 5.25
Bud Site -6.03 -2.75 -16.38 -14.57 -14.82 -5.37 -12.83 -9.37 -5.03 -0.18 -7.45 -13.15 -18.40 -14.99 -8.94
Cell Periphery 5.54 -0.70 -5.05 -5.25 -5.01 -2.40 -2.22 -3.68 -3.40 -0.86 2.57 -2.20 -6.01 -6.13 -4.42
Cytoplasm -1.61 12.67 12.43 14.19 -0.18 15.60 2.59 13.80 -1.93 11.73 6.36 8.33 18.92 11.45 11.03
Cytoplasmic Foci -3.10 22.00 17.92 20.91 -2.99 -6.72 29.13 17.35 18.83 -8.67 -4.64 36.26 25.25 26.49 -8.18
Eisosomes 4.91 9.62 -2.88 -5.64 -7.87 -1.93 4.65 3.41 5.56 -1.99 2.02 8.00 -0.13 -2.06 -7.61
Endoplasmic Reticulum 6.38 -12.33 -7.09 -10.92 4.80 0.92 -4.81 -3.89 -4.23 -0.81 5.82 -12.01 -8.34 -11.74 1.25
Endosome 4.06 -3.60 -4.45 -7.99 -1.02 -9.97 -1.72 -13.45 -2.97 -12.46 -4.09 -2.73 -13.45 -9.68 -11.36
Golgi -1.77 1.14 -9.90 -9.40 -10.29 -6.06 -0.89 -8.38 -3.75 -8.06 -5.43 -0.56 -12.68 -10.10 -12.39
Lipid Particles 3.97 -7.35 -8.79 -10.72 -2.75 1.96 -1.13 -3.86 -4.82 -3.13 4.40 -5.60 -9.33 -11.49 -5.47
Mitochondria -5.38 2.79 1.93 8.50 -1.86 -6.76 2.72 2.39 7.38 0.78 -8.86 3.62 3.20 10.78 0.36
Mitotic Spindle -1.54 -4.55 -6.76 -6.40 -1.65 -2.29 -8.86 -6.15 -3.61 -0.38 -2.72 -9.92 -9.05 -7.52 -0.95
None -4.87 -0.12 3.81 6.92 2.21 4.41 1.80 1.24 -1.08 0.35 -3.92 0.96 2.98 5.37 1.95
Nuclear Periphery -0.02 -9.98 1.46 1.08 10.36 -0.12 -6.19 0.71 0.34 6.78 -0.17 -11.34 1.62 0.98 12.00
Nuclear Periphery Foci 0.03 -12.79 -7.67 -7.63 9.60 4.75 -8.61 -5.03 -5.84 -0.12 0.65 -14.52 -9.05 -9.12 7.35
Nucleolus -7.14 -2.98 4.93 8.49 7.72 2.84 4.50 4.99 1.87 1.77 -6.38 1.19 7.31 8.40 7.21
Nucleus -3.60 -6.49 8.81 11.86 12.06 12.30 -3.80 14.72 0.66 17.25 4.69 -7.66 17.20 11.00 21.56
Peroxisomes -6.68 3.08 -3.71 1.75 -6.06 -7.39 -1.13 -3.54 6.22 -2.72 -9.98 0.53 -5.17 6.10 -5.48
Vacuole 8.03 -6.68 -6.38 -13.98 0.11 -2.83 -14.05 -17.70 -12.02 -7.33 3.04 -13.44 -17.42 -19.38 -5.99
Vacuole Periphery 0.20 1.07 0.28 0.15 -0.57 -6.96 1.55 -2.84 4.52 -4.17 -5.40 2.44 -1.89 2.68 -3.85
Standard name
Human Ortholog
Description Putative integral membrane protein of unknown function; interacts with Ulp1p at the nuclear periphery; member of DUP240 gene family
Localization
Cell Percentages cytoplasm (20%), vacuole (9%), mixed (40%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Uip3

Uip3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Uip3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available