Standard name
Human Ortholog
Description Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – 0 0 0 0 0.06 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – – – – –
Cell Periphery 0 0 0.05 0.05 0 0 0 0 0 0 0 0 – 0 0.05 – – – 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 – 0.06 0 – – – 0 0 0 0.05 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0.05 0.05 0.06 0.05 0 0 0 0 0 – 0 0 – – – 0 0.05 0.06 0 0 0
Golgi 0 0.12 0.27 0.26 0.27 0.06 0 0.06 0 0.05 0 0 – 0.42 0.46 – – – 0 0.07 0 0 0.13 0.11
Mitochondria 0.93 0.84 0.74 0.86 0.7 0.77 0.76 0.8 0.82 0.85 0.96 0.91 – 0.73 0.71 – – – 0.88 0.75 0.79 0.81 0.7 0.7
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0.07 0 0 0 0 0.1 0.12 0.12 0.1 0.09 0.08 0.09 – 0 0 – – – 0 0 0 0 0 0
Nucleolus 0.1 0 0 0 0.05 0.07 0.12 0.1 0.12 0.07 0.07 0.06 – 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0.05 0.05 0 0 0 0 0 0 0 0 – 0 0 – – – 0 0 0.05 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0.1 0.21 0.28 0.08 0.17 0.08 0.05 0 0.06 0.08 0 0 – 0.14 0.1 – – – 0 0.05 0 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 3 1 1 1 7 1 1 1 1 – 2 2 – – – 0 5 1 0 6 2
Bud 1 1 2 1 1 3 5 6 12 7 11 5 – 0 0 – – – 0 1 0 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – 0 1 0 0 0 1
Bud Site 0 0 0 0 0 0 0 1 3 1 0 0 – 0 0 – – – – – – – – –
Cell Periphery 1 12 4 8 5 4 4 6 2 8 3 8 – 1 7 – – – 0 0 0 0 0 0
Cytoplasm 1 3 1 0 5 4 7 3 2 4 4 6 – 8 6 – – – 0 0 0 1 0 1
Endoplasmic Reticulum 0 0 1 0 1 1 4 2 1 2 0 5 – 1 2 – – – 0 1 0 0 0 0
Endosome 0 8 2 8 13 17 23 16 7 11 0 7 – 5 3 – – – 2 15 6 1 1 2
Golgi 0 34 22 43 65 18 15 25 9 19 2 13 – 54 65 – – – 3 21 3 0 13 16
Mitochondria 105 234 60 144 167 230 324 348 252 345 338 404 – 95 100 – – – 96 235 91 27 76 99
Nucleus 1 1 0 0 0 1 2 2 1 2 0 0 – 0 0 – – – 0 0 0 1 0 0
Nuclear Periphery 8 6 0 3 10 29 53 51 32 38 27 41 – 2 4 – – – 0 2 0 0 1 0
Nucleolus 11 7 1 5 11 21 53 42 37 29 23 25 – 2 1 – – – 0 1 0 0 0 0
Peroxisomes 0 7 4 8 7 8 8 8 12 3 0 2 – 2 1 – – – 3 6 6 1 4 4
SpindlePole 0 0 2 5 2 5 4 7 8 7 1 8 – 1 1 – – – 0 2 0 0 0 0
Vac/Vac Membrane 11 58 23 14 40 25 22 18 20 32 14 13 – 18 14 – – – 1 17 4 0 1 8
Unique Cell Count 113 279 81 167 240 297 425 436 308 406 352 444 130 140 110 314 116 34 109 142
Labelled Cell Count 139 372 122 242 328 367 525 542 399 509 424 538 191 206 110 314 116 34 109 142


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.0 9.0 8.8 9.2 11.2 10.5 10.3 10.1 8.9 9.3 8.2 8.9 – 10.3 10.3 – – – – 10.2 11.2
Std Deviation (1e-4) 1.4 1.8 2.1 1.9 2.1 2.8 2.7 2.3 2.5 2.2 2.3 2.5 – 1.9 2.0 – – – – 1.8 2.3
Intensity Change (Log2) – – – 0.06 0.35 0.25 0.22 0.19 0.02 0.08 -0.11 0.01 – 0.23 0.22 – – – – 0.21 0.34


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 – – –
Bud 0 0 0 0 0 0 0 0 0 – 0 0 – – –
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 – – –
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 – – –
Cytoplasm 0 0 0 0 0 0 0 0 0 – 0 0 – – –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 – – –
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 – – –
Golgi -0.2 0 -5.5 -7.5 -6.2 -7.2 -6.7 0 -8.0 – 2.1 2.8 – – –
Mitochondria 2.3 -0.8 0.6 0.4 1.2 1.6 2.4 6.5 4.4 – -0.2 -0.4 – – –
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 – – –
Nuclear Periphery 0 0 2.9 3.4 3.2 3.0 2.9 2.6 2.8 – 0 0 – – –
Nucleolus 0 0 0 3.0 2.5 2.9 0 0 0 – 0 0 – – –
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 – – –
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 – – –
Vacuole -4.1 -2.3 -4.8 -6.7 -7.4 -5.6 -5.3 -7.1 -8.3 – -2.6 -3.5 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.232 7.8407 7.0653 5.7748 7.0644 7.5114 10.6347 10.151 9.8126 9.4347 9.0122 9.7958 6.7382 7.9698 7.2513 6.2413 5.8207 7.1173
Actin 0.0328 0.0012 0.014 0.0405 0.0001 0.0013 0.001 0.0016 0.0003 0 0.0001 0.0002 0.028 0.0012 0.0016 0.0597 0.0193 0.0005
Bud 0.0002 0.0008 0.0003 0.0001 0 0.0001 0 0 0 0 0 0 0.0006 0.0001 0.0003 0.0004 0.0005 0
Bud Neck 0.0028 0.0002 0.0003 0.0001 0 0.0001 0 0 0 0 0 0 0.0004 0.0001 0.0004 0.0004 0.0006 0
Bud Periphery 0.0003 0.0016 0.0007 0.0002 0 0.0002 0.0001 0 0.0001 0 0 0 0.0011 0.0001 0.0004 0.0004 0.0011 0.0002
Bud Site 0.0015 0.0021 0.0018 0.0002 0 0 0 0 0.0001 0 0 0 0.0013 0.0002 0.0017 0.0017 0.0007 0
Cell Periphery 0.0002 0.0008 0.0016 0.0001 0 0.0001 0.0001 0 0.0001 0 0 0 0.0003 0.0002 0.0001 0.0001 0.0002 0.0001
Cytoplasm 0.0015 0.0001 0.0026 0.0001 0 0.0001 0 0 0 0 0 0 0.0022 0 0.0028 0.0084 0.0003 0
Cytoplasmic Foci 0.028 0.0015 0.005 0.0029 0 0.0003 0.0007 0.0009 0.0017 0 0 0.0001 0.0187 0.0009 0.0162 0.0295 0.0105 0.0003
Eisosomes 0.0004 0.0002 0.0017 0.0002 0 0.0001 0.0001 0.0001 0.0001 0 0.0001 0.0001 0.0003 0.0001 0.0001 0.0003 0.0001 0.0001
Endoplasmic Reticulum 0.0015 0.0001 0.002 0.0002 0 0.0002 0 0 0 0 0 0 0.0011 0 0.0001 0.0139 0.0007 0
Endosome 0.0215 0.0094 0.0083 0.0066 0.0002 0.0026 0.0022 0.0014 0.0024 0.0005 0.0002 0.0009 0.0147 0.0034 0.0048 0.0375 0.0369 0.0015
Golgi 0.0157 0.0222 0.0114 0.0139 0.0004 0.0052 0.0092 0.0195 0.0049 0.0014 0.0006 0.0045 0.0222 0.0063 0.005 0.0127 0.0413 0.0017
Lipid Particles 0.026 0.0128 0.0053 0.0194 0.0001 0.0095 0.0034 0.0004 0.0017 0 0.0005 0.0006 0.0293 0.0131 0.0048 0.0116 0.0138 0.006
Mitochondria 0.8239 0.9176 0.9259 0.9091 0.9985 0.9604 0.9723 0.969 0.9811 0.9974 0.9969 0.9896 0.8221 0.9552 0.9404 0.7907 0.863 0.9736
None 0.0033 0.0001 0.0016 0.0002 0 0.0005 0 0 0 0 0 0 0.024 0 0.0001 0.0019 0.0001 0
Nuclear Periphery 0.0084 0.0001 0.0011 0.0002 0 0.007 0 0 0 0 0 0 0.0022 0 0.0002 0.0065 0.0006 0.0002
Nucleolus 0.0009 0.0004 0.001 0.0001 0 0.0006 0.0001 0 0.0011 0 0 0 0.0026 0.0004 0.0002 0.0001 0.0001 0.0001
Nucleus 0.001 0.0002 0.0006 0 0 0.0015 0 0 0.0002 0 0 0 0.0004 0.0001 0.0001 0.0008 0.0002 0.0001
Peroxisomes 0.013 0.0045 0.0059 0.003 0.0003 0.0011 0.0066 0.0061 0.0011 0.0001 0.0002 0.0022 0.0147 0.0046 0.0089 0.0158 0.003 0.0053
Punctate Nuclear 0.0077 0 0.0033 0.0002 0 0.0026 0 0 0.0004 0 0 0 0.002 0 0.0006 0.0049 0.0005 0
Vacuole 0.0029 0.012 0.002 0.0005 0.0001 0.0008 0.0006 0.0001 0.0017 0 0.0002 0.0001 0.0036 0.0053 0.0048 0.0015 0.0019 0.002
Vacuole Periphery 0.0064 0.0119 0.0037 0.002 0.0003 0.0056 0.0034 0.0007 0.0029 0.0004 0.0013 0.0015 0.008 0.0086 0.0063 0.0011 0.0046 0.0083

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 56.5173 44.5021 43.512 56.8005 51.256 43.901 49.7603 51.2705 55.9501 55.3619
Translational Efficiency 0.9982 1.0304 1.1399 0.8979 0.8808 1.1845 0.935 0.9341 1.0125 1.0044

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1313 1400 269 1226 1835 2437 1582 197 3148 3837 1851 1423

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 665.49 974.59 1530.30 1336.76 964.37 951.15 1418.85 1503.28 839.71 959.70 1435.05 1359.81
Standard Deviation 99.55 170.94 284.77 249.37 151.90 165.76 228.33 270.06 198.25 168.05 240.60 258.81
Intensity Change Log 2 — 0.550378 1.201326 1.006252 — -0.019914 0.557063 0.640455 — 0.240665 0.855551 0.801163

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000771 0.001826 0.013784 0.013454 0.001059 0.001861 0.004089 0.009586 0.000939 0.001848 0.005498 0.012919
Bud Neck 0.002514 0.000841 0.007063 0.001231 0.000927 0.001265 0.001840 0.002823 0.001589 0.001110 0.002599 0.001452
Bud Site 0.008117 0.002563 0.009208 0.002431 0.001046 0.002233 0.001827 0.003226 0.003995 0.002354 0.002900 0.002541
Cell Periphery 0.001322 0.000179 0.000228 0.000219 0.000124 0.000262 0.000107 0.002042 0.000623 0.000232 0.000124 0.000472
Cytoplasm 0.001150 0.000726 0.000721 0.001162 0.000222 0.000492 0.000617 0.000632 0.000609 0.000577 0.000632 0.001089
Cytoplasmic Foci 0.005182 0.019444 0.034362 0.007668 0.008034 0.011656 0.023351 0.010726 0.006844 0.014498 0.024952 0.008091
Eisosomes 0.000046 0.000087 0.000425 0.000459 0.000086 0.000081 0.000158 0.000623 0.000069 0.000083 0.000197 0.000482
Endoplasmic Reticulum 0.000536 0.000196 0.000409 0.000256 0.000115 0.000143 0.000188 0.000846 0.000291 0.000162 0.000220 0.000338
Endosome 0.017001 0.017288 0.012118 0.005152 0.010323 0.019454 0.008697 0.013912 0.013108 0.018663 0.009194 0.006365
Golgi 0.010129 0.027471 0.049593 0.028807 0.012493 0.019892 0.038362 0.050518 0.011507 0.022657 0.039994 0.031813
Lipid Particles 0.004398 0.004340 0.007924 0.001331 0.005285 0.004595 0.008282 0.000644 0.004915 0.004502 0.008230 0.001236
Mitochondria 0.909170* 0.889694* 0.792048* 0.912050* 0.938976* 0.913993* 0.877201* 0.848983* 0.926544* 0.905127* 0.864826* 0.903319*
Mitotic Spindle 0.001529 0.002500 0.017513 0.002664 0.000883 0.001139 0.006117 0.002656 0.001152 0.001635 0.007773 0.002663
None 0.000494 0.000307 0.002076 0.000752 0.000338 0.000342 0.001107 0.001247 0.000403 0.000329 0.001248 0.000820
Nuclear Periphery 0.000077 0.000103 0.001789 0.000133 0.000146 0.000119 0.001272 0.000325 0.000117 0.000113 0.001348 0.000160
Nuclear Periphery Foci 0.000121 0.000235 0.000932 0.000101 0.000213 0.000264 0.000720 0.000278 0.000175 0.000254 0.000751 0.000126
Nucleolus 0.000148 0.000656 0.000380 0.000253 0.000148 0.000199 0.000467 0.001591 0.000148 0.000366 0.000454 0.000439
Nucleus 0.000478 0.000433 0.000122 0.000139 0.000086 0.000097 0.000112 0.000450 0.000250 0.000219 0.000113 0.000182
Peroxisomes 0.020558 0.023771 0.022197 0.015829 0.012867 0.014020 0.007100 0.023343 0.016075 0.017578 0.009294 0.016869
Vacuole 0.007232 0.003657 0.011887 0.002250 0.003083 0.004402 0.006441 0.010875 0.004813 0.004131 0.007233 0.003444
Vacuole Periphery 0.009029 0.003684 0.015219 0.003657 0.003546 0.003492 0.011945 0.014674 0.005833 0.003562 0.012421 0.005182

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.43 -3.87 -6.81 -6.18 0.09 -1.70 -4.41 -2.27 -2.05 -1.45 -2.94 -6.17 -7.06 -6.51 -4.05
Bud Neck 2.97 -3.08 2.14 -1.70 4.14 -0.80 -2.94 -2.34 -1.79 -1.19 1.35 -2.64 0.33 -1.14 3.15
Bud Site 3.44 -0.37 3.63 0.15 2.21 -1.87 -1.52 -2.39 -0.99 -1.47 2.12 1.23 1.76 -0.30 0.50
Cell Periphery 4.11 3.94 3.98 -0.84 0.19 -2.49 0.61 -1.31 -1.22 -1.32 3.23 4.27 0.65 -1.16 -1.70
Cytoplasm 0.96 1.13 -0.04 -0.67 -0.72 -2.64 -1.78 -2.80 -0.79 -0.04 0.20 -0.06 -0.85 -0.93 -0.81
Cytoplasmic Foci -8.27 -6.83 -2.26 6.63 6.22 -3.12 -8.85 -1.05 0.35 4.32 -7.94 -11.61 -1.30 5.68 10.12
Eisosomes -2.73 -7.06 -5.21 -4.62 -0.35 0.27 -4.35 -2.84 -2.86 -2.46 -1.19 -9.17 -5.61 -5.41 -3.85
Endoplasmic Reticulum 3.32 1.17 2.73 -1.12 2.30 -0.89 -2.94 -2.07 -1.98 -1.86 2.71 1.58 -0.68 -2.78 -1.93
Endosome -0.14 1.98 6.57 7.64 3.49 -5.10 1.18 -0.96 1.45 -1.43 -3.99 3.28 5.39 9.31 2.57
Golgi -7.22 -6.01 -6.06 -0.38 2.95 -4.05 -9.68 -3.91 -3.15 -1.23 -7.72 -11.84 -6.96 -3.04 2.29
Lipid Particles 0.04 -2.09 2.90 3.41 4.74 0.64 -2.27 5.44 5.74 7.37 0.50 -3.01 5.24 5.52 7.43
Mitochondria 2.33 6.39 -0.33 -2.63 -6.52 4.27 8.71 4.65 3.35 1.42 4.42 9.42 3.53 0.29 -4.80
Mitotic Spindle -1.89 -3.92 -1.28 -0.20 3.56 -0.97 -4.50 -2.38 -2.04 2.57 -1.90 -5.71 -1.99 -1.35 3.70
None 2.34 -7.04 -2.19 -4.83 5.75 -0.08 -14.34 -6.27 -5.92 -0.92 1.53 -13.32 -4.79 -5.64 4.37
Nuclear Periphery -1.18 -5.35 -1.77 -0.90 5.16 0.91 -5.31 -1.63 -1.89 4.00 0.20 -6.59 -1.33 -1.46 6.29
Nuclear Periphery Foci -1.77 -3.99 0.42 1.96 4.06 -0.75 -3.75 -0.72 -0.13 2.86 -1.65 -4.91 1.26 2.46 5.26
Nucleolus -1.28 -3.18 -0.75 0.86 0.54 -0.79 -2.42 -1.06 -1.02 -0.82 -1.45 -2.72 -1.30 -0.34 -0.03
Nucleus 0.17 1.55 1.35 0.99 -0.32 -0.28 -0.50 -0.93 -0.90 -0.86 0.26 1.32 0.46 0.19 -0.80
Peroxisomes -0.91 -0.28 1.47 2.40 1.12 -0.58 3.28 -1.46 -1.30 -2.29 -0.82 3.90 -0.33 0.30 -3.26
Vacuole 3.32 -1.52 4.44 2.01 3.25 -2.02 -4.38 -2.42 -2.01 -1.36 1.12 -2.92 1.66 0.92 4.04
Vacuole Periphery 5.00 -2.23 4.81 0.04 4.38 0.10 -7.30 -3.08 -3.11 -0.73 4.16 -6.00 0.78 -2.33 6.12
Standard name
Human Ortholog
Description Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss
Localization
Cell Percentages mitochondrion (97%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Aim9

Aim9


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Aim9-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available