Standard name | |
---|---|
Human Ortholog | |
Description | Mannosyltransferase, involved in N-linked glycosylation; catalyzes the transfer of both the seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; human homolog ALG9 can complement yeast null mutant; mutation of human homolog causes type 1 congenital disorders of glycosylation |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 0 | 0 | 3 | 4 | 1 | 5 | 15 | – | 8 | 6 | 18 | 7 | 18 | 21 | 6 | 5 | 6 | 0 | 0 | 2 | 4 | 7 | 5 |
Bud | 0 | 0 | 0 | 1 | 1 | 3 | 1 | 3 | – | 8 | 4 | 9 | 1 | 3 | 0 | 1 | 0 | 0 | 2 | 3 | 0 | 4 | 3 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 2 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 4 | 4 | 10 | 1 | 3 | 4 | – | 12 | 12 | 20 | 14 | 36 | 65 | 8 | 4 | 7 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 4 | 20 | 28 | 28 | 25 | 30 | 44 | – | 35 | 40 | 36 | 12 | 45 | 70 | 17 | 21 | 8 | 0 | 0 | 0 | 0 | 0 | 3 |
Endoplasmic Reticulum | 23 | 25 | 63 | 33 | 42 | 47 | 63 | 115 | – | 77 | 56 | 84 | 147 | 357 | 314 | 2 | 4 | 3 | 62 | 54 | 176 | 276 | 396 | 465 |
Endosome | 0 | 0 | 3 | 2 | 1 | 2 | 6 | 2 | – | 3 | 2 | 4 | 0 | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 4 | 4 |
Golgi | 0 | 0 | 1 | 3 | 7 | 1 | 0 | 3 | – | 2 | 0 | 0 | 10 | 17 | 8 | 0 | 5 | 10 | 1 | 0 | 1 | 3 | 3 | 5 |
Mitochondria | 2 | 4 | 18 | 37 | 21 | 47 | 56 | 95 | – | 137 | 149 | 142 | 1 | 0 | 1 | 5 | 10 | 6 | 3 | 1 | 5 | 10 | 12 | 9 |
Nucleus | 2 | 0 | 1 | 6 | 5 | 2 | 3 | 11 | – | 9 | 11 | 5 | 13 | 18 | 13 | 4 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 95 | 40 | 140 | 170 | 146 | 83 | 94 | 101 | – | 65 | 54 | 77 | 283 | 283 | 144 | 20 | 23 | 9 | 90 | 56 | 67 | 73 | 54 | 44 |
Nucleolus | 1 | 0 | 2 | 1 | 1 | 0 | 2 | 4 | – | 3 | 0 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | – | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 75 | 42 | 62 | 84 | 161 | 106 | 129 | 176 | – | 125 | 92 | 82 | 280 | 300 | 415 | 19 | 30 | 6 | 5 | 11 | 6 | 8 | 18 | 42 |
Unique Cell Count | 147 | 90 | 243 | 304 | 353 | 266 | 332 | 455 | 384 | 343 | 364 | 611 | 829 | 861 | 72 | 84 | 44 | 171 | 133 | 266 | 388 | 505 | 594 | |
Labelled Cell Count | 204 | 115 | 314 | 372 | 427 | 319 | 393 | 574 | 486 | 427 | 480 | 770 | 1080 | 1058 | 82 | 103 | 56 | 171 | 133 | 266 | 388 | 505 | 594 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.8 | 11.8 | 10.6 | 11.3 | 11.7 | 10.5 | 10.2 | 9.9 | – | 8.9 | 9.0 | 9.2 | 13.4 | 12.3 | 13.4 | 10.1 | 11.3 | 15.2 | 13.8 | 15.2 | 15.6 |
Std Deviation (1e-4) | 2.3 | 2.2 | 2.2 | 2.4 | 2.6 | 2.3 | 2.2 | 2.1 | – | 1.9 | 2.0 | 2.1 | 2.2 | 2.0 | 2.3 | 2.4 | 2.7 | 3.3 | 2.6 | 2.9 | 2.7 |
Intensity Change (Log2) | – | – | – | 0.1 | 0.14 | -0.0 | -0.04 | -0.09 | – | -0.25 | -0.24 | -0.2 | 0.34 | 0.21 | 0.35 | -0.06 | 0.09 | 0.52 | 0.39 | 0.52 | 0.56 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 2.9 | – | 0 | 0 | 3.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0.9 | 0 | 0 | 0 | – | 1.1 | 1.4 | 2.4 | 0.6 | 2.0 | 3.4 | 0 | 0 | 0 |
Cytoplasm | 0.4 | -0.1 | 0.5 | 0.3 | 0.6 | – | 0.4 | 1.3 | 0.7 | -4.4 | -1.6 | -0.1 | 3.6 | 4.0 | 0 |
Endoplasmic Reticulum | -4.6 | -4.4 | -2.3 | -2.0 | -0.2 | – | -1.7 | -2.8 | -0.8 | -0.6 | 4.8 | 3.1 | -4.3 | -4.1 | -2.8 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1.8 | -0.7 | 3.5 | 3.3 | 4.6 | – | 8.0 | 9.5 | 8.7 | -6.5 | 0 | 0 | -0.1 | 1.3 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | -0.4 | -3.9 | -6.0 | -7.1 | -9.4 | – | -10.6 | -10.6 | -9.2 | -3.0 | -6.6 | -12.9 | -4.4 | -4.8 | -4.5 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.6 | 5.0 | 3.4 | 3.4 | 3.5 | – | 1.9 | 0.4 | -0.8 | 5.5 | 3.1 | 6.3 | 0.1 | 1.8 | -1.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 12.5379 | 14.0675 | 13.8725 | 12.8501 | 11.8643 | 13.2849 | 16.6412 | 15.3933 | 14.0771 | 14.2509 | 14.6686 | 14.0952 | 16.3561 | 14.9628 | 15.0644 | 14.6327 | 13.7943 | 14.173 |
Actin | 0.0131 | 0.0001 | 0.0092 | 0.0178 | 0.0022 | 0.001 | 0.0197 | 0 | 0.0051 | 0.0002 | 0.0166 | 0.0019 | 0.0012 | 0.0011 | 0.004 | 0.0005 | 0.0036 | 0.0003 |
Bud | 0.0002 | 0 | 0.0001 | 0.0003 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 |
Bud Neck | 0.0053 | 0.0001 | 0.0006 | 0.0014 | 0.0007 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0005 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0011 | 0 |
Bud Periphery | 0.0003 | 0 | 0.0002 | 0.0007 | 0.0007 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0005 | 0.0003 | 0.0004 | 0.0002 | 0 |
Bud Site | 0.0029 | 0 | 0.0008 | 0.0015 | 0.0012 | 0 | 0.0006 | 0 | 0.0005 | 0 | 0.0005 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0011 | 0 |
Cell Periphery | 0.0003 | 0.0003 | 0.0016 | 0.0006 | 0.0006 | 0 | 0.0004 | 0.0002 | 0.0004 | 0.0002 | 0.0006 | 0.0001 | 0.0002 | 0.0076 | 0.0007 | 0.0003 | 0.0001 | 0 |
Cytoplasm | 0.022 | 0.0036 | 0.0015 | 0.0063 | 0.0013 | 0.0019 | 0.0155 | 0.0001 | 0.0031 | 0.0003 | 0.0091 | 0.0001 | 0.0003 | 0.0079 | 0.0002 | 0.0002 | 0.0054 | 0.0001 |
Cytoplasmic Foci | 0.0126 | 0.0001 | 0.0003 | 0.0071 | 0.003 | 0.0011 | 0.0034 | 0 | 0.0159 | 0 | 0.0304 | 0.0012 | 0.0035 | 0.0002 | 0.001 | 0 | 0.0123 | 0.0032 |
Eisosomes | 0.0003 | 0 | 0.0006 | 0.0001 | 0.0019 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0964 | 0.3155 | 0.2833 | 0.3306 | 0.2253 | 0.073 | 0.1152 | 0.3835 | 0.2995 | 0.3919 | 0.3424 | 0.125 | 0.0789 | 0.3235 | 0.3927 | 0.3985 | 0.204 | 0.1037 |
Endosome | 0.0221 | 0.0011 | 0.0015 | 0.0081 | 0.0069 | 0.0048 | 0.0023 | 0.0002 | 0.0139 | 0.0007 | 0.0056 | 0.0016 | 0.0075 | 0.0025 | 0.001 | 0.0011 | 0.0207 | 0.0003 |
Golgi | 0.0038 | 0.0001 | 0.0006 | 0.0026 | 0.003 | 0.0004 | 0.0043 | 0 | 0.0074 | 0 | 0.0021 | 0.001 | 0.0055 | 0.0001 | 0.0013 | 0.0001 | 0.0093 | 0.0002 |
Lipid Particles | 0.0096 | 0.0009 | 0.003 | 0.0098 | 0.015 | 0.0014 | 0.0054 | 0.0001 | 0.0069 | 0.0001 | 0.0019 | 0.0013 | 0.0092 | 0.0004 | 0.002 | 0.0001 | 0.0212 | 0.0005 |
Mitochondria | 0.002 | 0.0002 | 0.0005 | 0.0014 | 0.0347 | 0.0002 | 0.0012 | 0.0002 | 0.0008 | 0.0008 | 0.0004 | 0.0003 | 0.0025 | 0.0006 | 0.0043 | 0.0009 | 0.0025 | 0.0001 |
None | 0.0051 | 0 | 0.0055 | 0.0011 | 0.0526 | 0.0004 | 0.0037 | 0 | 0.0016 | 0 | 0.0018 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.001 | 0 |
Nuclear Periphery | 0.7462 | 0.6131 | 0.6137 | 0.5596 | 0.5577 | 0.8636 | 0.7693 | 0.5564 | 0.5703 | 0.5498 | 0.527 | 0.8238 | 0.8298 | 0.5755 | 0.5258 | 0.5463 | 0.6414 | 0.848 |
Nucleolus | 0.0008 | 0.0004 | 0.0008 | 0.0001 | 0.0036 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0007 | 0 |
Nucleus | 0.0123 | 0.0291 | 0.0408 | 0.0223 | 0.029 | 0.0231 | 0.0047 | 0.0019 | 0.011 | 0.004 | 0.0019 | 0.0016 | 0.0075 | 0.0076 | 0.004 | 0.0055 | 0.0078 | 0.0033 |
Peroxisomes | 0.0018 | 0 | 0.0001 | 0.0005 | 0.0159 | 0.001 | 0.0026 | 0 | 0.001 | 0 | 0.0045 | 0.0009 | 0.0004 | 0 | 0.0048 | 0 | 0.0114 | 0.0028 |
Punctate Nuclear | 0.0145 | 0.0004 | 0.0018 | 0.0014 | 0.0011 | 0.004 | 0.0003 | 0 | 0.0027 | 0 | 0.0236 | 0.0004 | 0.0002 | 0.0004 | 0.0001 | 0 | 0.0132 | 0.0045 |
Vacuole | 0.0045 | 0.0098 | 0.0092 | 0.0041 | 0.0108 | 0.0027 | 0.0034 | 0.0047 | 0.0071 | 0.0053 | 0.0037 | 0.0018 | 0.0034 | 0.0201 | 0.0064 | 0.0056 | 0.0062 | 0.0015 |
Vacuole Periphery | 0.0239 | 0.0251 | 0.0242 | 0.0224 | 0.0327 | 0.0211 | 0.0474 | 0.0525 | 0.0524 | 0.0465 | 0.0268 | 0.039 | 0.0494 | 0.0513 | 0.0511 | 0.0402 | 0.0365 | 0.0313 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 60.8592 | 57.3612 | 43.0033 | 53.5475 | 51.4609 | 40.9911 | 47.6838 | 47.0139 | 38.2332 | 58.4515 |
Translational Efficiency | 0.7032 | 0.6372 | 0.7373 | 0.4651 | 0.5448 | 1.0422 | 0.7234 | 0.6317 | 0.7339 | 0.5276 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
86 | 1014 | 253 | 117 | 213 | 2009 | 2021 | 1359 | 299 | 3023 | 2274 | 1476 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1333.86 | 1164.03 | 1524.61 | 1405.51 | 1120.46 | 1257.04 | 1385.23 | 1215.29 | 1181.84 | 1225.84 | 1400.74 | 1230.37 |
Standard Deviation | 163.81 | 232.01 | 239.85 | 232.77 | 214.32 | 217.25 | 212.94 | 203.77 | 223.09 | 226.61 | 220.50 | 212.53 |
Intensity Change Log 2 | — | -0.196479 | 0.192833 | 0.075486 | — | 0.165939 | 0.306034 | 0.117209 | — | -0.019679 | 0.245616 | 0.094684 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.002437 | 0.000471 | 0.000808 | 0.000463 | 0.000368 | 0.000339 | 0.000236 | 0.001249 | 0.000963 | 0.000383 | 0.000300 | 0.001187 |
Bud Neck | 0.003243 | 0.000910 | 0.004089 | 0.004644 | 0.001424 | 0.000614 | 0.000985 | 0.003269 | 0.001947 | 0.000713 | 0.001330 | 0.003378 |
Bud Site | 0.009319 | 0.002659 | 0.001521 | 0.003809 | 0.003423 | 0.000987 | 0.001221 | 0.005582 | 0.005119 | 0.001548 | 0.001255 | 0.005442 |
Cell Periphery | 0.001795 | 0.000885 | 0.000413 | 0.000629 | 0.000384 | 0.000298 | 0.000232 | 0.000549 | 0.000789 | 0.000495 | 0.000252 | 0.000555 |
Cytoplasm | 0.013534 | 0.009067 | 0.001775 | 0.014207 | 0.007623 | 0.007439 | 0.010776 | 0.023230 | 0.009323 | 0.007985 | 0.009775 | 0.022514 |
Cytoplasmic Foci | 0.015024 | 0.002022 | 0.000710 | 0.003033 | 0.002981 | 0.001300 | 0.000549 | 0.004955 | 0.006445 | 0.001542 | 0.000567 | 0.004803 |
Eisosomes | 0.000177 | 0.000008 | 0.000240 | 0.000058 | 0.000016 | 0.000006 | 0.000005 | 0.000047 | 0.000062 | 0.000007 | 0.000031 | 0.000048 |
Endoplasmic Reticulum | 0.564441* | 0.375212* | 0.231468* | 0.501039* | 0.520824* | 0.274287* | 0.513226* | 0.580679* | 0.533370* | 0.308141* | 0.481878* | 0.574366* |
Endosome | 0.024299 | 0.014311 | 0.018028 | 0.018745 | 0.012467 | 0.008012 | 0.009130 | 0.022108 | 0.015870 | 0.010125 | 0.010120 | 0.021842 |
Golgi | 0.006984 | 0.000855 | 0.000888 | 0.001982 | 0.001238 | 0.000332 | 0.000137 | 0.004987 | 0.002891 | 0.000508 | 0.000220 | 0.004749 |
Lipid Particles | 0.056239 | 0.003453 | 0.003484 | 0.006467 | 0.005068 | 0.002215 | 0.000855 | 0.008886 | 0.019786 | 0.002630 | 0.001147 | 0.008694 |
Mitochondria | 0.002074 | 0.003488 | 0.000601 | 0.000289 | 0.001963 | 0.003424 | 0.001087 | 0.002761 | 0.001995 | 0.003445 | 0.001033 | 0.002565 |
Mitotic Spindle | 0.009035 | 0.001770 | 0.003186 | 0.002144 | 0.000254 | 0.001393 | 0.001925 | 0.011184 | 0.002780 | 0.001519 | 0.002065 | 0.010467 |
None | 0.000005 | 0.001925 | 0.000164 | 0.000052 | 0.000397 | 0.002679 | 0.000318 | 0.001097 | 0.000284 | 0.002426 | 0.000300 | 0.001014 |
Nuclear Periphery | 0.073144 | 0.360379* | 0.462573* | 0.155302* | 0.243217* | 0.544022* | 0.329962* | 0.099541 | 0.194300* | 0.482423* | 0.344716* | 0.103961* |
Nuclear Periphery Foci | 0.129896 | 0.201233* | 0.255152* | 0.249245* | 0.181637 | 0.125542 | 0.111891 | 0.198013* | 0.166755 | 0.150931 | 0.127830 | 0.202074* |
Nucleolus | 0.000049 | 0.001015 | 0.000208 | 0.000129 | 0.000153 | 0.000787 | 0.000125 | 0.000240 | 0.000123 | 0.000864 | 0.000134 | 0.000231 |
Nucleus | 0.000889 | 0.004188 | 0.000788 | 0.002325 | 0.006062 | 0.011905 | 0.008970 | 0.004919 | 0.004574 | 0.009317 | 0.008060 | 0.004713 |
Peroxisomes | 0.000357 | 0.000291 | 0.000024 | 0.000092 | 0.000295 | 0.000301 | 0.000071 | 0.000202 | 0.000312 | 0.000298 | 0.000065 | 0.000193 |
Vacuole | 0.080806 | 0.010878 | 0.011489 | 0.032921 | 0.006615 | 0.010359 | 0.007457 | 0.022692 | 0.027954 | 0.010533 | 0.007906 | 0.023503 |
Vacuole Periphery | 0.006252 | 0.004981 | 0.002390 | 0.002424 | 0.003592 | 0.003757 | 0.000843 | 0.003811 | 0.004357 | 0.004168 | 0.001015 | 0.003701 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.84 | 1.50 | 1.73 | -0.55 | 0.29 | 0.07 | 0.77 | -2.10 | -2.18 | -2.36 | 1.79 | 1.91 | -0.27 | -2.14 | -2.26 |
Bud Neck | 3.25 | -0.39 | -0.55 | -1.85 | -0.31 | 0.79 | 0.32 | -2.58 | -5.75 | -4.85 | 1.87 | 0.83 | -2.46 | -5.97 | -4.53 |
Bud Site | 2.32 | 2.59 | 1.73 | -1.01 | -1.56 | 1.23 | 1.07 | -1.44 | -4.64 | -4.38 | 2.30 | 2.43 | -0.45 | -4.36 | -4.54 |
Cell Periphery | 1.55 | 2.71 | 2.09 | 0.40 | -1.02 | 0.67 | 1.03 | -1.02 | -2.33 | -2.74 | 1.47 | 2.97 | 1.19 | -0.50 | -2.83 |
Cytoplasm | 1.15 | 3.10 | -0.09 | -0.81 | -1.94 | 0.08 | -0.88 | -3.91 | -7.24 | -5.57 | 0.51 | -0.19 | -4.03 | -7.14 | -6.03 |
Cytoplasmic Foci | 2.99 | 3.32 | 2.52 | -0.55 | -1.27 | 1.08 | 1.61 | -1.31 | -5.01 | -6.45 | 2.88 | 3.53 | 0.79 | -4.78 | -6.56 |
Eisosomes | 2.25 | -0.07 | 1.52 | -1.91 | 0.93 | 1.38 | 1.55 | -1.51 | -2.01 | -2.07 | 2.41 | 1.28 | 0.72 | -2.23 | -0.70 |
Endoplasmic Reticulum | 4.41 | 7.62 | 1.15 | -3.45 | -7.16 | 10.88 | 0.37 | -2.16 | -25.00 | -4.78 | 11.18 | 2.38 | -1.71 | -23.43 | -7.09 |
Endosome | 1.74 | 0.99 | 0.83 | -1.64 | -0.24 | 2.29 | 1.67 | -4.57 | -11.05 | -10.58 | 2.66 | 2.59 | -2.72 | -10.04 | -9.99 |
Golgi | 1.73 | 1.72 | 1.27 | -0.77 | -0.75 | 1.13 | 1.37 | -2.78 | -4.46 | -4.67 | 2.03 | 2.29 | -1.23 | -4.38 | -4.70 |
Lipid Particles | 3.47 | 3.46 | 3.23 | -1.63 | -1.35 | 2.08 | 3.13 | -2.54 | -6.39 | -7.86 | 3.67 | 3.96 | 2.29 | -6.29 | -7.84 |
Mitochondria | -0.74 | 0.74 | 0.91 | 4.32 | 3.24 | -2.87 | 2.36 | -0.93 | 0.76 | -1.99 | -2.17 | 1.49 | -0.64 | 1.07 | -1.98 |
Mitotic Spindle | 1.08 | 0.81 | 1.02 | -0.33 | 0.30 | -2.24 | -2.56 | -5.74 | -4.92 | -4.52 | 0.58 | 0.25 | -3.13 | -4.93 | -4.41 |
None | -2.37 | -1.62 | -2.52 | 2.31 | 1.07 | -3.51 | 0.45 | -2.08 | 2.27 | -2.56 | -4.18 | -0.20 | -2.47 | 2.48 | -2.55 |
Nuclear Periphery | -16.58* | -17.33 | -3.42 | 9.91 | 12.20 | -16.30* | -4.70 | 8.29 | 51.58* | 28.42* | -19.48 | -10.04 | 6.40 | 50.76* | 31.16 |
Nuclear Periphery Foci | -3.42 | -5.41 | -3.83 | -1.92 | 0.29 | 4.38 | 5.42 | -1.12 | -9.62 | -11.33 | 1.43 | 3.49 | -2.79 | -7.06 | -10.04 |
Nucleolus | -2.75 | -1.99 | -1.16 | 2.44 | 0.62 | -4.35 | 0.21 | -2.00 | 3.19 | -2.09 | -4.93 | -0.61 | -2.49 | 3.63 | -1.99 |
Nucleus | -3.32 | 0.61 | -1.45 | 1.01 | -1.62 | -1.49 | -0.86 | 0.06 | 3.91 | 2.55 | -1.67 | -1.34 | -0.34 | 3.18 | 2.28 |
Peroxisomes | 0.46 | 2.61 | 1.78 | 2.28 | -1.28 | -0.12 | 2.38 | 0.86 | 0.76 | -2.08 | 0.08 | 3.04 | 1.36 | 1.12 | -2.26 |
Vacuole | 4.57 | 4.53 | 2.27 | -2.61 | -2.55 | -1.82 | -0.59 | -6.31 | -6.10 | -7.47 | 3.53 | 3.97 | 0.60 | -6.70 | -7.82 |
Vacuole Periphery | 0.26 | 0.91 | 0.87 | 2.19 | -0.07 | -0.17 | 3.11 | -0.16 | -0.02 | -4.23 | 0.10 | 2.50 | 0.43 | 0.72 | -4.13 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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