Standard name
Human Ortholog
Description Assembly factor for the F0 sector of mitochondrial F1F0 ATP synthase; mitochondrial inner membrane protein; interacts genetically with ATP6
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0.05 0 0 – 0 0.05 0 0 0 0 0.07 0.09 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.12 0.06 0.06 0 0 0 0 0 0
Cytoplasm 0.53 0.37 0.57 0.6 0.56 0.49 0.44 0.46 – 0.44 0.42 0.32 0.81 0.83 0.89 0.17 0.09 0.1 0.15 0.16 0.17 0.18 0.16 0.18
Endoplasmic Reticulum 0 0.15 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0.1 0 0 0.12 0.12 0.14 0 0.13 0.07 0 0.11 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.08 0.12 0.24 0 0 0 0 0 0
Mitochondria 0.48 0.57 0.48 0.48 0.46 0.61 0.64 0.62 – 0.66 0.71 0.76 0.1 0.1 0 0.2 0.14 0.06 0.61 0.53 0.56 0.56 0.41 0.45
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.22 0.14 0.08 0 0 0 0 0 0
SpindlePole 0 0 0.05 0 0.06 0 0.05 0.07 – 0.05 0.05 0.05 0 0 0 0.14 0.06 0.12 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0 0 0 0 0 0 0 – 0 0 0 0.05 0 0.05 0.12 0.32 0.24 0 0.07 0 0.06 0.11 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 1 0 0 0 0 0 – 1 0 0 0 1 0 3 0 2 5 2 4 2 2 5
Bud 6 0 6 4 3 16 14 21 – 11 19 19 0 2 0 5 6 2 3 2 0 1 3 4
Bud Neck 2 0 2 0 1 4 5 4 – 3 5 1 0 0 0 0 0 0 1 0 1 0 1 2
Bud Site 0 0 2 0 1 2 7 7 – 10 4 5 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 0 1 1 1 1 2 1 – 2 0 2 0 1 1 9 4 3 0 0 3 0 0 2
Cytoplasm 86 20 106 120 167 164 185 263 – 207 164 158 17 104 115 13 6 5 28 13 39 14 14 24
Endoplasmic Reticulum 2 8 2 0 0 0 1 0 – 0 0 3 0 1 3 1 4 4 2 0 5 1 0 2
Endosome 0 1 1 2 13 0 1 2 – 2 0 0 2 5 4 9 8 7 8 11 15 2 10 5
Golgi 1 0 0 0 0 0 0 0 – 0 0 0 0 0 0 6 8 12 3 1 0 0 2 1
Mitochondria 79 31 89 97 135 206 265 358 – 315 275 373 2 13 5 15 9 3 112 44 132 44 39 60
Nucleus 1 0 0 0 3 7 5 5 – 5 1 1 0 0 1 1 2 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 1 – 2 1 1 0 0 0 0 0 1 0 0 1 0 0 0
Nucleolus 0 0 0 0 0 1 2 1 – 0 1 2 0 0 0 0 1 2 0 0 0 0 0 0
Peroxisomes 1 0 1 2 5 0 0 2 – 1 0 1 0 2 0 17 9 4 2 0 3 1 1 1
SpindlePole 3 0 10 6 17 13 19 42 – 22 19 23 0 0 1 11 4 6 5 1 2 1 3 3
Vac/Vac Membrane 8 1 1 0 3 3 2 3 – 4 4 3 1 3 6 9 21 12 5 5 9 4 10 14
Unique Cell Count 163 54 186 200 296 337 416 575 475 387 493 21 125 129 76 66 50 186 84 236 80 96 135
Labelled Cell Count 193 63 222 232 349 417 509 710 585 493 592 22 132 136 99 82 63 186 84 236 80 96 135


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 4.1 3.1 3.0 3.0 3.0 2.6 2.7 – 2.5 2.5 2.5 4.6 4.7 5.4 6.7 6.8 6.9 6.4 5.4 6.1
Std Deviation (1e-4) 1.4 0.9 1.0 1.0 0.8 0.9 1.4 1.0 – 0.9 1.1 0.9 0.8 1.1 2.4 1.8 1.5 1.5 3.8 3.1 2.6
Intensity Change (Log2) – – – -0.06 -0.09 -0.08 -0.29 -0.2 – -0.31 -0.32 -0.31 0.55 0.59 0.78 1.09 1.11 1.13 1.01 0.79 0.95


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 –
Bud Neck – – – – – – – – – – – – – 0 –
Bud Site – – – – – – – – – – – – – 0 –
Cell Periphery – – – – – – – – – – – – – 0 –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 –
Endosome – – – – – – – – – – – – – 0 –
Golgi – – – – – – – – – – – – – 0 –
Mitochondria – – – – – – – – – – – – – 0 –
Nuclear Periphery – – – – – – – – – – – – – 0 –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 –
Peroxisomes – – – – – – – – – – – – – 0 –
SpindlePole – – – – – – – – – – – – – 0 –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 –
Cytoplasm – – – – – – – – – – – – – 0 –
Nucleus – – – – – – – – – – – – – 0 –
Vacuole – – – – – – – – – – – – – 7.7 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.4666 -0.1862 -0.2042 -0.5721 -0.7213 -0.2527 1.8284 1.9795 2.0012 1.7386 1.9552 1.9917 -1.1275 -0.7671 -0.8998 -0.6961 -1.2346 -0.8551
Actin 0.0224 0.0071 0.0189 0.0165 0.0144 0.0066 0.0406 0.0078 0.0141 0.0214 0.0257 0.019 0.0163 0.0055 0.0108 0.0128 0.0065 0.0078
Bud 0.0016 0.0107 0.0019 0.0046 0.0032 0.0012 0.0019 0.0023 0.0027 0.0049 0.0098 0.0012 0.0008 0.003 0.0023 0.0035 0.0044 0.0008
Bud Neck 0.0014 0.0011 0.0053 0.0014 0.0058 0.0053 0.005 0.001 0.0012 0.0354 0.0005 0.005 0.0014 0.0037 0.0018 0.0012 0.0021 0.0052
Bud Periphery 0.0048 0.0143 0.0065 0.0159 0.0131 0.0034 0.0044 0.0045 0.0072 0.0398 0.0667 0.0047 0.0022 0.0071 0.0085 0.0198 0.015 0.0023
Bud Site 0.0085 0.0178 0.0098 0.0031 0.0066 0.001 0.0167 0.0152 0.0094 0.0092 0.0031 0.0014 0.0048 0.025 0.0074 0.0014 0.0031 0.0016
Cell Periphery 0.0012 0.0016 0.0041 0.0038 0.0012 0.0006 0.0023 0.0008 0.005 0.0131 0.0017 0.0015 0.0012 0.0017 0.003 0.0006 0.0017 0.001
Cytoplasm 0.027 0.0499 0.0395 0.0434 0.0864 0.0532 0.0226 0.0216 0.0386 0.0223 0.0416 0.0191 0.0323 0.059 0.0412 0.0716 0.1039 0.0547
Cytoplasmic Foci 0.1113 0.0837 0.0854 0.0523 0.0984 0.1019 0.096 0.0522 0.063 0.087 0.0686 0.0647 0.1082 0.1113 0.0773 0.0522 0.0834 0.102
Eisosomes 0.0021 0.0008 0.0017 0.0008 0.0007 0.0004 0.0013 0.0004 0.0028 0.0094 0.0015 0.0012 0.0019 0.0011 0.0019 0.0006 0.0013 0.0006
Endoplasmic Reticulum 0.0051 0.0035 0.0054 0.0031 0.0044 0.0023 0.0065 0.0009 0.0033 0.0017 0.0056 0.0013 0.0051 0.0029 0.0037 0.0024 0.0018 0.0025
Endosome 0.1177 0.1002 0.0897 0.0985 0.0543 0.0856 0.1232 0.0561 0.0699 0.0747 0.1291 0.0609 0.1172 0.0856 0.0584 0.0538 0.0847 0.0883
Golgi 0.0346 0.0324 0.0282 0.0314 0.0272 0.0314 0.0624 0.0539 0.0445 0.0623 0.0497 0.0571 0.0373 0.0371 0.0274 0.023 0.0509 0.0414
Lipid Particles 0.0545 0.0209 0.032 0.0238 0.0103 0.04 0.0419 0.0034 0.0144 0.012 0.0079 0.0265 0.0739 0.0343 0.0214 0.0033 0.0253 0.0317
Mitochondria 0.2655 0.3723 0.3416 0.4534 0.3185 0.3734 0.4316 0.6072 0.5295 0.4182 0.3059 0.5761 0.3527 0.324 0.4229 0.5268 0.3993 0.3952
None 0.2509 0.201 0.2529 0.1557 0.263 0.1798 0.0595 0.0901 0.0853 0.0523 0.1398 0.0918 0.1466 0.2164 0.2295 0.2043 0.1579 0.1758
Nuclear Periphery 0.005 0.003 0.0062 0.0013 0.0042 0.0049 0.0035 0.0003 0.0023 0.0004 0.0443 0.0006 0.0056 0.0011 0.0012 0.001 0.0152 0.0015
Nucleolus 0.0033 0.0022 0.0026 0.0011 0.0017 0.0014 0.0013 0.0006 0.0006 0.0022 0.0005 0.0007 0.0018 0.0013 0.0013 0.0004 0.0007 0.0009
Nucleus 0.0036 0.0036 0.0031 0.0022 0.005 0.0029 0.0085 0.0006 0.0011 0.0016 0.0163 0.0006 0.0023 0.0014 0.0014 0.001 0.0013 0.0012
Peroxisomes 0.058 0.0572 0.0457 0.0666 0.0657 0.0881 0.0495 0.0745 0.0934 0.1169 0.068 0.0569 0.0628 0.0644 0.0639 0.0101 0.0167 0.0716
Punctate Nuclear 0.0049 0.002 0.0031 0.0009 0.0082 0.0025 0.0011 0.0002 0.0006 0.001 0.0009 0.0017 0.004 0.0011 0.0014 0.0006 0.0033 0.0051
Vacuole 0.0116 0.0114 0.0112 0.0132 0.0055 0.0093 0.0133 0.0043 0.007 0.0092 0.0077 0.0048 0.015 0.0096 0.0099 0.0059 0.0144 0.0058
Vacuole Periphery 0.0051 0.0034 0.005 0.0069 0.002 0.0049 0.0068 0.0022 0.004 0.0048 0.0051 0.003 0.0066 0.0033 0.0034 0.0039 0.0071 0.003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.8518 7.0455 11.3275 9.205 4.2578 14.4413 10.6856 14.0235 10.8756 10.685
Translational Efficiency 1.4782 1.6452 1.1258 1.2653 2.3081 1.0533 1.4758 1.1233 1.5215 1.1566

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2301 1979 127 1345 1843 1553 2449 1882 4144 3532 2576 3227

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 651.03 743.79 906.38 834.22 668.36 672.95 814.20 859.60 658.74 712.64 818.74 849.02
Standard Deviation 82.79 93.46 87.58 120.46 86.20 86.85 124.90 104.47 84.76 97.20 124.93 112.12
Intensity Change Log 2 — 0.192171 0.477392 0.357704 — 0.009874 0.284758 0.363040 — 0.102704 0.383024 0.360409

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000531 0.000917 0.002341 0.007329 0.000431 0.000778 0.001483 0.001266 0.000486 0.000856 0.001525 0.003793
Bud Neck 0.061027 0.057658 0.011085 0.029772 0.047566 0.060496 0.051627 0.044296 0.055040 0.058906 0.049628 0.038242
Bud Site 0.016753 0.031687 0.015706 0.071587 0.015082 0.022937 0.038716 0.046181 0.016010 0.027840 0.037582 0.056770
Cell Periphery 0.000284 0.000227 0.000299 0.000273 0.000148 0.000298 0.000136 0.000212 0.000223 0.000259 0.000144 0.000237
Cytoplasm 0.269698* 0.231097* 0.122548* 0.263764* 0.301383* 0.146090* 0.309998* 0.257937* 0.283790* 0.193720* 0.300756* 0.260366*
Cytoplasmic Foci 0.292040* 0.282220* 0.148347 0.114649 0.330935* 0.296038* 0.073219 0.112011 0.309338* 0.288296* 0.076923 0.113110
Eisosomes 0.000502 0.000204 0.000206 0.000140 0.000263 0.000299 0.000069 0.000105 0.000396 0.000246 0.000076 0.000120
Endoplasmic Reticulum 0.002097 0.000924 0.010091 0.003596 0.001356 0.000694 0.002828 0.001998 0.001767 0.000823 0.003186 0.002664
Endosome 0.042507 0.046678 0.057900 0.032690 0.041037 0.068069 0.020526 0.048213 0.041853 0.056083 0.022369 0.041743
Golgi 0.020033 0.031112 0.018157 0.016915 0.016867 0.035616 0.010069 0.016433 0.018625 0.033092 0.010468 0.016634
Lipid Particles 0.028450 0.010830 0.019062 0.003787 0.017303 0.017240 0.003444 0.006624 0.023492 0.013648 0.004214 0.005441
Mitochondria 0.066261 0.096616 0.023774 0.034492 0.037226 0.176649* 0.044903 0.071160 0.053348 0.131806* 0.043862 0.055877
Mitotic Spindle 0.006683 0.015609 0.041210 0.065411 0.002713 0.010559 0.073628 0.050653 0.004918 0.013388 0.072030 0.056804
None 0.002792 0.001710 0.003831 0.001932 0.002640 0.001388 0.003623 0.002720 0.002724 0.001568 0.003633 0.002392
Nuclear Periphery 0.000694 0.000392 0.002941 0.001889 0.000486 0.000262 0.002571 0.001819 0.000601 0.000335 0.002590 0.001848
Nuclear Periphery Foci 0.000422 0.000517 0.005638 0.001540 0.000374 0.000330 0.000998 0.000784 0.000400 0.000435 0.001227 0.001099
Nucleolus 0.002552 0.001813 0.008594 0.001933 0.002432 0.002173 0.004717 0.003722 0.002498 0.001971 0.004908 0.002976
Nucleus 0.087566 0.068316 0.311018* 0.128676 0.087967 0.037290 0.227023* 0.124981 0.087745 0.054674 0.231164* 0.126521
Peroxisomes 0.023993 0.043929 0.042824 0.052507 0.019387 0.069244 0.025716 0.024932 0.021945 0.055060 0.026559 0.036425
Vacuole 0.070777 0.071629 0.138127 0.162902 0.070237 0.046342 0.099385 0.174065 0.070537 0.060511 0.101295 0.169412
Vacuole Periphery 0.004339 0.005915 0.016299 0.004215 0.004166 0.007208 0.005320 0.009889 0.004262 0.006483 0.005861 0.007524

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.31 -5.04 -5.26 -4.79 -2.75 -3.04 -4.56 -2.84 -1.41 0.85 -3.49 -4.93 -5.88 -4.88 -3.18
Bud Neck 1.05 16.76 11.69 9.90 -6.26 -3.31 -0.82 1.62 4.88 2.61 -1.43 2.52 8.58 9.40 4.90
Bud Site -7.39 0.49 -10.80 -6.46 -9.81 -4.17 -9.30 -9.83 -6.39 -2.36 -8.42 -9.33 -14.41 -8.52 -5.40
Cell Periphery 1.28 -0.72 1.49 0.07 2.39 -5.14 2.22 -2.23 3.20 -3.37 -1.19 4.29 0.81 2.11 -4.39
Cytoplasm 4.99 10.10 0.08 -4.15 -9.56 19.62 -0.17 3.80 -14.96 4.10 16.12 -1.48 2.49 -12.10 3.52
Cytoplasmic Foci 1.44 9.62 32.93 29.70 2.72 4.38 45.59 35.80 28.61 -9.69 4.03 56.66 48.19 41.11 -9.05
Eisosomes 5.41 4.73 6.84 6.37 4.40 -1.78 13.53 11.05 13.19 -7.67 4.70 10.30 9.12 14.48 -8.70
Endoplasmic Reticulum 4.60 -6.16 -4.41 -9.70 5.17 3.69 -8.69 -3.24 -7.77 5.95 5.81 -8.33 -4.63 -12.26 4.33
Endosome -1.58 -1.66 7.47 8.51 4.23 -7.78 10.51 -0.41 7.41 -10.84 -6.68 13.33 3.86 9.83 -8.49
Golgi -5.20 0.88 5.01 8.96 0.86 -8.53 6.17 1.90 9.81 -3.74 -9.47 8.84 4.92 13.00 -3.34
Lipid Particles 10.21 2.07 15.74 8.98 5.07 -0.01 13.40 9.58 8.06 -4.78 8.16 19.02 17.72 10.96 -2.11
Mitochondria -5.70 6.10 10.63 14.85 -0.41 -19.67 -0.15 -4.91 15.40 -5.01 -18.08 5.47 2.71 19.25 -2.36
Mitotic Spindle -4.41 -2.61 -11.25 -8.80 -1.37 -4.55 -18.00 -13.05 -10.01 4.29 -6.22 -17.64 -16.92 -13.03 3.73
None 2.13 -0.25 2.00 -0.21 0.78 2.83 -1.44 0.29 -1.92 1.47 3.33 -1.36 1.30 -1.73 2.76
Nuclear Periphery 1.76 -4.55 -3.61 -4.72 1.54 3.03 -12.27 -5.44 -6.42 3.10 2.66 -11.66 -6.14 -7.79 3.52
Nuclear Periphery Foci -0.40 -3.75 -5.65 -4.33 3.12 0.72 -5.90 -3.46 -3.70 2.55 -0.21 -6.50 -6.20 -4.87 2.15
Nucleolus 2.09 -2.73 1.92 -0.12 3.14 0.69 -4.11 -2.02 -2.78 2.11 2.03 -4.68 -0.83 -2.67 3.97
Nucleus 4.51 -7.90 -10.66 -13.49 5.54 13.87 -26.10 -9.57 -20.05 15.21 11.61 -28.67 -14.22 -22.42 16.52
Peroxisomes -8.77 -2.19 -4.44 1.46 0.26 -14.82 -1.87 -1.57 13.09 0.22 -16.89 -1.07 -4.02 10.85 -2.40
Vacuole -0.59 -5.49 -21.99 -21.60 -4.00 7.19 -11.52 -21.80 -26.55 -12.29 3.88 -13.42 -30.92 -33.07 -16.61
Vacuole Periphery -3.58 -2.26 3.11 5.43 2.58 -4.42 -0.43 -4.95 -1.28 -4.86 -5.68 -1.32 -3.59 0.70 -1.93
Standard name
Human Ortholog
Description Assembly factor for the F0 sector of mitochondrial F1F0 ATP synthase; mitochondrial inner membrane protein; interacts genetically with ATP6
Localization
Cell Percentages mitochondrion (19%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Atp10

Atp10


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Atp10-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available