Standard name
Human Ortholog
Description Cytochrome c1; component of the mitochondrial respiratory chain; expression is regulated by the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.12 0.05 0.13 0.09 0.08 0 0.06 0 0.08
Bud 0 0 0 0 0 0 0 0 – 0 0 0.05 0 0 0 0 0.07 0.05 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.29 0 0 0 0 0 0 0 0
Cytoplasm 0.06 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0.1 0.09 0 0 0 0 0 0 0 0 0
Golgi 0.05 0 0 0 0 0 0 0 – 0 0 0 0.21 0.26 0.25 0.47 0.33 0.29 0.07 0.11 0.06 0.1 0.15 0.13
Mitochondria 0.73 0.92 0.92 0.96 0.9 0.87 0.88 0.87 – 0.91 0.93 0.91 0.78 0.74 0.65 0 0.07 0.16 0.66 0.68 0.68 0.6 0.57 0.55
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0.07 0.09 0.08 – 0.08 0.08 0.08 0 0 0 0.06 0.05 0.08 0 0 0 0 0 0
Nucleolus 0.12 0 0.05 0 0.07 0.12 0.13 0.15 – 0.12 0.12 0.14 0 0 0 0 0.05 0 0 0 0 0 0 0
Peroxisomes 0 0.06 0.2 0 0 0 0 0 – 0 0 0 0.11 0 0.09 0.12 0.22 0.08 0 0.05 0.19 0.15 0.16 0.12
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Vac/Vac Membrane 0.08 0.09 0 0 0.09 0 0 0 – 0 0 0 0.1 0.12 0.18 0.12 0.14 0.24 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 3 0 1 0 1 0 3 – 1 0 0 2 1 3 2 3 5 6 29 6 8 7 14
Bud 0 5 8 2 6 14 14 13 – 5 10 17 1 2 2 0 4 2 0 1 0 0 0 3
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 1 0 0 0 6 5 2 – 6 6 2 1 0 0 0 0 0 – – – – – –
Cell Periphery 2 15 3 2 3 0 1 1 – 1 1 0 3 3 7 5 2 1 0 2 0 0 0 0
Cytoplasm 4 3 4 1 2 6 6 4 – 3 2 6 3 3 1 1 1 0 0 2 0 0 0 0
Endoplasmic Reticulum 1 1 0 0 1 11 5 4 – 4 6 2 0 3 0 0 0 0 0 0 0 0 0 0
Endosome 1 1 6 3 7 0 3 4 – 2 0 1 6 14 19 0 1 1 0 12 10 5 6 5
Golgi 3 15 0 2 1 2 0 0 – 0 2 1 29 37 51 8 19 11 5 38 16 14 25 26
Mitochondria 47 316 250 151 219 318 426 525 – 410 334 341 107 106 136 0 4 6 48 253 192 86 99 106
Nucleus 0 0 0 0 0 3 3 4 – 4 0 0 0 0 2 0 3 0 0 0 0 0 0 0
Nuclear Periphery 1 10 11 3 10 24 44 46 – 37 28 31 2 3 3 1 3 3 1 1 0 1 2 2
Nucleolus 8 5 14 5 18 44 65 88 – 54 42 52 5 2 5 0 3 1 1 0 0 1 0 1
Peroxisomes 0 20 55 7 8 4 19 15 – 12 5 5 15 6 18 2 13 3 1 20 53 21 27 22
SpindlePole 2 1 4 2 0 1 8 6 – 9 3 1 2 1 7 0 3 0 2 0 0 1 0 0
Vac/Vac Membrane 5 30 4 4 22 7 7 14 – 10 5 6 14 17 38 2 8 9 0 3 0 1 1 7
Unique Cell Count 64 343 273 158 243 366 483 605 453 361 373 137 143 208 17 58 38 74 370 285 144 175 195
Labelled Cell Count 74 426 359 183 297 441 606 729 558 444 465 190 198 292 21 67 42 74 370 285 144 175 195


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 18.4 14.4 9.6 9.8 9.3 9.3 9.1 10.0 – 10.3 11.2 11.6 10.6 10.6 10.2 14.7 14.0 14.6 12.3 12.9 13.0
Std Deviation (1e-4) 8.6 3.9 2.9 4.6 3.3 3.6 3.5 3.8 – 4.3 4.7 4.5 2.7 2.8 4.6 3.7 3.8 3.9 3.6 4.4 3.9
Intensity Change (Log2) – – – 0.03 -0.04 -0.04 -0.08 0.07 – 0.1 0.22 0.27 0.14 0.14 0.1 0.62 0.55 0.61 0.36 0.43 0.45


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 -0.3 0.6 0 -0.7 – 0 -0.1 1.1 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Endosome 0 0.5 0 0 0 – 0 0 0 0 3.4 3.4 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 7.9 8.8 8.7 0 0 0
Mitochondria 1.6 -0.6 -1.9 -1.4 -2.0 – -0.5 0.4 -0.1 -3.8 -4.8 -7.1 -10.6 -13.9 -11.5
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery -1.2 0 1.4 2.6 2.0 – 2.2 1.9 2.2 0 0 -1.7 0 0 0
Nucleolus -1.0 1.1 3.0 3.6 4.0 – 3.0 2.9 3.7 -0.7 -1.9 -1.5 0 0 0
Peroxisomes -4.5 -5.8 -8.2 -7.2 -8.9 – -7.9 -8.0 -8.1 -2.3 -4.4 -3.5 0 0.4 -1.8
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0 3.9 0 0 0.8 – 0.7 0 0 4.1 4.6 6.5 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 11.1776 12.1125 11.6069 9.9511 10.705 11.1356 20.5656 18.6902 19.8332 20.0645 18.5859 18.9104 18.2679 16.5956 18.7008 16.5511 18.0826 17.5973
Actin 0.0159 0.03 0.0186 0.008 0.027 0.0066 0.0121 0.0126 0.0128 0.0004 0.0015 0.0033 0.0099 0.0091 0.0034 0.0167 0.0027 0.0109
Bud 0.0002 0.0011 0.0006 0.0002 0.0005 0.0008 0 0 0 0 0 0 0 0.0001 0 0 0 0
Bud Neck 0.0003 0.0003 0.0003 0.0001 0.0004 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Periphery 0.0004 0.0012 0.0014 0.0004 0.002 0.0033 0.0001 0.0001 0 0 0 0 0.0001 0.0001 0 0 0 0
Bud Site 0.001 0.0033 0.0076 0.0009 0.0007 0.0001 0.0001 0.0001 0.0001 0 0 0 0.0001 0.0001 0 0 0 0
Cell Periphery 0.0002 0.0003 0.0002 0.0001 0.0006 0.0001 0.0001 0.0001 0 0 0 0 0.0001 0.0001 0.0001 0 0 0.0001
Cytoplasm 0.0013 0.0002 0.0015 0.0006 0.0002 0 0 0 0.0001 0 0 0 0 0 0 0 0 0
Cytoplasmic Foci 0.0133 0.0088 0.0065 0.0106 0.0109 0.0017 0.0016 0.0023 0.001 0.0001 0.0011 0.0003 0.001 0.0025 0.0011 0.0006 0.0001 0.001
Eisosomes 0.0005 0.0006 0.0006 0.0004 0.0003 0.0004 0.0009 0.0003 0.0003 0.0002 0.0004 0.0005 0.0009 0.0003 0.0004 0.0006 0.001 0.0005
Endoplasmic Reticulum 0.0019 0.0002 0.0004 0.0003 0.0002 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.0201 0.0166 0.0075 0.026 0.0159 0.0038 0.0017 0.006 0.0012 0.0001 0.0026 0.0014 0.0026 0.0078 0.0028 0.0036 0.0011 0.0028
Golgi 0.043 0.0709 0.0557 0.1123 0.0668 0.0315 0.0139 0.0446 0.0069 0.0012 0.0227 0.0224 0.0209 0.0405 0.0237 0.0191 0.016 0.0332
Lipid Particles 0.0325 0.0135 0.0167 0.019 0.0272 0.0144 0.0142 0.0053 0.0038 0.0004 0.0202 0.0014 0.0161 0.0199 0.016 0.0027 0.0004 0.0126
Mitochondria 0.7951 0.8174 0.8498 0.7827 0.8166 0.9144 0.9346 0.8913 0.9628 0.9925 0.9367 0.9658 0.9328 0.9008 0.9435 0.9228 0.9761 0.9212
None 0.0005 0 0.0002 0.0003 0.0001 0 0 0 0.0001 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.0077 0.0001 0.0034 0.0003 0.0003 0 0.0001 0 0.0001 0 0 0 0.0001 0 0 0 0 0
Nucleolus 0.0045 0.0003 0.0005 0.0001 0.0001 0.0002 0.0003 0 0 0 0 0 0.0004 0 0 0 0 0
Nucleus 0.0015 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0 0 0 0 0 0.0001 0 0 0 0 0
Peroxisomes 0.0433 0.0285 0.0227 0.0311 0.018 0.0198 0.014 0.0333 0.0079 0.005 0.0126 0.0041 0.0096 0.0134 0.004 0.0307 0.0017 0.0099
Punctate Nuclear 0.0043 0 0.0002 0.0005 0.0002 0 0.0001 0 0.0011 0 0 0 0 0 0 0 0 0
Vacuole 0.0045 0.0018 0.001 0.0015 0.0027 0.0006 0.0011 0.0006 0.0003 0 0.0002 0.0001 0.0009 0.0005 0.0006 0.0003 0.0001 0.0005
Vacuole Periphery 0.0079 0.0049 0.0044 0.0044 0.0091 0.0023 0.0053 0.0035 0.0015 0.0001 0.0018 0.0006 0.0043 0.0048 0.0041 0.0028 0.0009 0.007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 24.3224 14.8487 20.4627 41.8909 26.151 29.645 26.0055 26.7803 33.4365 22.2665
Translational Efficiency 1.2112 1.6758 1.8003 1.3819 1.4049 1.054 1.053 1.6765 1.4155 1.2344

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1696 2126 1606 1697 2434 1895 2715 1978 4130 4021 4321 3675

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 917.45 998.00 1717.81 1388.46 821.49 999.40 1447.93 1421.36 860.90 998.66 1548.24 1406.17
Standard Deviation 235.13 226.53 487.93 314.68 150.30 251.13 318.87 331.98 195.56 238.44 411.56 324.52
Intensity Change Log 2 — 0.121410 0.904869 0.597784 — 0.282819 0.817677 0.790957 — 0.199915 0.864335 0.692270

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.015292 0.020813 0.004930 0.010251 0.017356 0.019387 0.002115 0.006925 0.016509 0.020141 0.003161 0.008461
Bud Neck 0.001355 0.000938 0.001565 0.000767 0.001896 0.000878 0.000830 0.001490 0.001674 0.000910 0.001103 0.001156
Bud Site 0.002484 0.003623 0.001352 0.003917 0.006508 0.001592 0.001520 0.010479 0.004856 0.002666 0.001457 0.007449
Cell Periphery 0.000266 0.000215 0.000114 0.000362 0.000518 0.000146 0.000085 0.000714 0.000414 0.000183 0.000096 0.000552
Cytoplasm 0.000938 0.000905 0.000336 0.000786 0.002893 0.000636 0.000538 0.001653 0.002090 0.000778 0.000463 0.001253
Cytoplasmic Foci 0.020540 0.015575 0.011074 0.007705 0.040565 0.025582 0.007432 0.008226 0.032342 0.020291 0.008786 0.007985
Eisosomes 0.000613 0.000592 0.000627 0.000249 0.000248 0.000668 0.000161 0.000288 0.000398 0.000628 0.000335 0.000270
Endoplasmic Reticulum 0.000263 0.000153 0.000117 0.000473 0.000248 0.000206 0.000084 0.000426 0.000255 0.000178 0.000096 0.000448
Endosome 0.020962 0.015190 0.002124 0.008038 0.069418 0.016580 0.005453 0.015004 0.049519 0.015845 0.004216 0.011787
Golgi 0.048633 0.042511 0.017836 0.050903 0.069946 0.064773 0.018305 0.034556 0.061194 0.053002 0.018131 0.042105
Lipid Particles 0.024200 0.007302 0.006223 0.001002 0.018302 0.013001 0.003210 0.002841 0.020724 0.009988 0.004330 0.001992
Mitochondria 0.816419* 0.841586* 0.915856* 0.888221* 0.667140* 0.813728* 0.938805* 0.879576* 0.728442* 0.828457* 0.930275* 0.883568*
Mitotic Spindle 0.001153 0.001538 0.001781 0.003943 0.003644 0.001169 0.002600 0.003300 0.002621 0.001364 0.002296 0.003597
None 0.001150 0.000492 0.003933 0.000755 0.000533 0.000643 0.001116 0.000808 0.000786 0.000563 0.002163 0.000783
Nuclear Periphery 0.000192 0.000089 0.001122 0.000170 0.000061 0.000119 0.000498 0.000791 0.000115 0.000103 0.000730 0.000504
Nuclear Periphery Foci 0.000179 0.000095 0.000377 0.000110 0.000161 0.000171 0.000191 0.000430 0.000168 0.000131 0.000260 0.000282
Nucleolus 0.000124 0.000058 0.000560 0.000082 0.000215 0.000079 0.000194 0.000155 0.000178 0.000068 0.000330 0.000122
Nucleus 0.000077 0.000073 0.000093 0.000028 0.000118 0.000030 0.000055 0.000102 0.000101 0.000053 0.000069 0.000068
Peroxisomes 0.041242 0.045900 0.004204 0.005402 0.096911 0.037162 0.005041 0.008277 0.074050 0.041782 0.004730 0.006949
Vacuole 0.001418 0.001033 0.006794 0.006581 0.001751 0.001532 0.004441 0.011017 0.001614 0.001268 0.005316 0.008969
Vacuole Periphery 0.002499 0.001320 0.018982 0.010255 0.001569 0.001920 0.007327 0.012940 0.001951 0.001603 0.011659 0.011700

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.44 5.85 2.43 5.02 -3.40 -0.96 11.12 6.45 6.58 -5.09 -2.35 12.36 6.24 8.32 -6.15
Bud Neck 1.22 -0.61 1.66 0.71 3.23 3.26 3.55 1.22 -2.70 -3.13 3.30 2.42 2.13 -1.67 -0.35
Bud Site -1.13 1.59 -1.66 -0.40 -3.50 5.50 5.50 -2.47 -6.25 -6.26 3.09 5.57 -2.70 -5.23 -7.08
Cell Periphery 1.13 3.79 -1.37 -2.28 -4.05 4.75 5.55 -1.22 -4.02 -4.45 4.62 6.52 -1.46 -4.50 -5.62
Cytoplasm 0.15 2.40 0.61 0.69 -2.61 4.39 3.96 2.10 -3.40 -2.63 4.10 4.38 2.34 -2.68 -3.06
Cytoplasmic Foci 3.19 6.05 9.34 6.16 2.65 7.05 19.70 18.63 10.72 -0.84 8.82 19.91 20.72 12.04 1.08
Eisosomes 0.26 -0.17 5.54 6.23 6.32 -5.94 4.56 -1.26 5.06 -4.01 -4.60 1.81 3.92 7.76 2.22
Endoplasmic Reticulum 2.49 3.55 -1.98 -3.20 -3.61 1.22 5.45 -2.71 -3.55 -5.78 2.80 6.44 -3.21 -4.75 -6.33
Endosome 2.98 11.63 7.20 5.18 -6.71 18.88* 24.38 18.82 0.89 -6.70 18.08 26.23 20.12 3.58 -8.57
Golgi 1.72 10.01 -0.52 -2.03 -8.83 1.28 17.86 10.13 7.61 -5.88 3.04 20.20 6.89 3.86 -10.47
Lipid Particles 7.45 7.96 10.94 7.56 6.45 2.72 9.43 9.55 7.78 0.57 7.42 12.42 14.55 10.41 4.86
Mitochondria -2.82 -12.14 -8.02 -5.79 3.88 -15.06 -34.48* -23.41 -7.67 9.35 -14.76 -34.58* -23.64 -9.39 9.94
Mitotic Spindle -1.02 -1.58 -3.68 -3.07 -2.60 5.15 1.50 0.48 -3.68 -0.90 3.76 0.64 -1.88 -4.63 -2.24
None 3.32 -9.90 1.98 -5.85 15.55 -0.42 -2.29 -1.09 -2.60 4.00 1.35 -7.35 0.03 -5.70 15.00
Nuclear Periphery 2.11 -3.84 0.41 -3.33 3.98 -4.02 -5.22 -2.51 -2.31 -0.97 0.54 -5.87 -2.46 -2.55 1.20
Nuclear Periphery Foci 1.94 -2.94 1.83 -0.52 4.54 -0.06 -0.74 -2.62 -2.14 -2.39 0.96 -2.69 -1.95 -2.36 -0.38
Nucleolus 1.95 -4.88 1.14 -1.03 5.54 1.90 0.52 0.99 -2.72 0.96 2.40 -2.15 1.34 -2.90 5.10
Nucleus 0.09 -0.57 1.99 1.35 2.24 3.85 2.17 0.67 -3.51 -1.62 2.12 1.31 1.75 -0.77 0.26
Peroxisomes -1.12 12.00 11.54 13.65 -1.14 12.91 23.83 22.35 9.96 -2.40 9.94 26.28 24.98 16.63 -2.52
Vacuole 1.24 -6.82 -5.07 -5.49 0.16 0.73 -5.29 -8.65 -8.74 -5.64 1.63 -8.52 -9.88 -10.30 -4.34
Vacuole Periphery 4.22 -11.01 -7.42 -8.72 4.86 -1.99 -9.66 -10.90 -10.50 -4.70 2.28 -14.33 -13.18 -13.71 -0.05
Standard name
Human Ortholog
Description Cytochrome c1; component of the mitochondrial respiratory chain; expression is regulated by the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex
Localization
Cell Percentages mitochondrion (93%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Cyt1

Cyt1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cyt1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available