Standard name
Human Ortholog
Description Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.06 0.08 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.08 0 0 0.06 0.11 0.08
Bud 0 0 0 0.06 0 0 0.05 0 0.08 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.52 0.4 0.76 0.43 0.69 0.45 0.25 0.2 0.09 0.09 0.11 0.09 0.15 0.14 0.22 0.16 0.12 0.19 0.21 0.16 0.18 0.08 0.11 0.18
Endoplasmic Reticulum 0.14 0.32 0.12 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0.17 0.16 0.13 0.05 0.05 0.07
Endosome 0 0.06 0 0.08 0.07 0.15 0.09 0.08 0 0 0 0 0.22 0.39 0.45 0.51 0.52 0.53 0.13 0.23 0.08 0.24 0.2 0.16
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0
Mitochondria 0.47 0.24 0 0.17 0.08 0.36 0.6 0.67 0.84 0.84 0.89 0.83 0.18 0.07 0.05 0.12 0.13 0.12 0.25 0.24 0.4 0.26 0.25 0.29
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0.18 0.21 0 0 0.07 0 0 0 0.12 0.09 0
SpindlePole 0 0 0.08 0.27 0.07 0.1 0.12 0.13 0.1 0.07 0.06 0.05 0.44 0.44 0.34 0.25 0.22 0.17 0 0 0.05 0.06 0.06 0.05
Vac/Vac Membrane 0 0.06 0 0 0 0.08 0.06 0.06 0 0.07 0 0.08 0 0 0 0.16 0.16 0.12 0.05 0.06 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 5 2 7 6 0 0 1 2 1 0 0 11 3 1 6 6 5 13 2 3 12 67 53
Bud 2 3 0 11 6 4 23 17 29 14 19 9 8 15 7 1 7 1 0 0 0 2 10 14
Bud Neck 1 0 0 3 11 1 1 2 0 1 0 0 2 6 5 0 1 0 1 0 1 3 12 10
Bud Site 0 0 0 0 1 1 5 2 5 5 5 6 0 0 0 0 0 1 – – – – – –
Cell Periphery 0 0 1 2 1 0 0 0 1 1 0 0 1 1 1 1 3 0 1 0 1 0 3 5
Cytoplasm 77 36 19 79 216 125 108 80 34 32 32 31 33 78 121 25 25 34 37 18 16 15 68 123
Endoplasmic Reticulum 21 29 3 7 13 0 3 1 2 5 2 10 6 0 1 5 12 12 29 18 12 9 33 45
Endosome 1 5 0 15 23 41 41 31 12 5 1 11 49 214 242 82 104 95 23 26 6 44 130 107
Golgi 0 1 0 1 1 0 0 0 0 0 0 0 0 1 0 1 1 5 0 6 0 5 14 10
Mitochondria 70 22 0 31 26 99 261 275 303 310 257 276 40 41 27 19 26 22 44 27 36 49 163 196
Nucleus 0 1 0 1 3 4 6 8 10 9 9 10 0 2 4 0 2 0 0 0 1 0 0 0
Nuclear Periphery 3 1 0 0 0 0 2 0 5 3 3 8 1 0 0 1 0 1 1 1 0 2 3 3
Nucleolus 0 2 0 0 1 2 4 2 11 2 6 2 9 11 11 2 3 7 0 0 0 0 4 2
Peroxisomes 0 0 0 3 1 8 6 7 2 1 1 2 30 100 113 1 9 13 4 2 0 23 57 29
SpindlePole 2 1 2 49 22 27 53 52 37 27 18 17 99 242 184 40 44 30 5 2 4 10 41 35
Vac/Vac Membrane 0 5 0 3 14 21 26 24 15 25 9 25 3 24 23 25 32 22 7 7 1 4 14 17
Unique Cell Count 148 90 25 184 314 276 433 410 362 367 290 331 226 554 539 160 200 180 176 115 91 189 644 677
Labelled Cell Count 179 111 27 212 345 333 539 502 468 441 362 407 292 738 740 209 275 248 176 115 91 189 644 677


Lipid Particles

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.4 4.9 5.0 5.1 5.2 4.4 4.2 4.8 4.1 4.2 3.8 4.3 5.8 6.0 5.7 6.4 6.8 6.6 5.2 5.6 5.6
Std Deviation (1e-4) 0.4 0.8 0.5 1.4 1.5 1.3 1.5 1.5 1.0 1.0 0.9 0.8 1.4 1.5 1.7 1.3 1.2 1.3 1.5 1.9 1.7
Intensity Change (Log2) – – – 0.02 0.04 -0.2 -0.26 -0.07 -0.31 -0.26 -0.4 -0.22 0.2 0.25 0.18 0.36 0.44 0.4 0.05 0.15 0.14


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.4251 0.9336 0.6119 0.7664 0.2638 0.8275 1.095 1.66 1.4098 1.3013 0.6523 1.0786 0.2752 0.4244 0.3833 0.7473 0.8237 0.4147
Actin 0.0007 0.0004 0.002 0.0027 0.0003 0.0003 0.0348 0.0022 0.0155 0.0348 0.0101 0.0004 0.0188 0.0006 0.0241 0.045 0.0402 0.0014
Bud 0.0035 0.0044 0.0001 0.0021 0 0.0013 0.0006 0.0002 0.0002 0.0014 0.0002 0 0.0003 0 0.0007 0.0006 0.0011 0.0001
Bud Neck 0.0003 0.0004 0.0037 0.0006 0.0001 0.0005 0.0027 0.0003 0.0008 0.0256 0.0006 0.0004 0.0007 0.0003 0.0066 0.0009 0.0009 0.0004
Bud Periphery 0.0014 0.0005 0.0003 0.0011 0.0001 0.0006 0.0012 0.0003 0.0002 0.0025 0.0004 0.0002 0.0004 0 0.0009 0.0008 0.0018 0.0002
Bud Site 0.0005 0.0073 0.0031 0.0016 0.0001 0.0003 0.0026 0.0084 0.0023 0.0084 0.0008 0.0001 0.0014 0.0005 0.0083 0.0017 0.0062 0.0006
Cell Periphery 0.0003 0.0002 0.0003 0.0014 0 0.0002 0.0005 0.0001 0.0001 0.0007 0.0001 0.0001 0.0002 0.0001 0.0003 0.0001 0.0003 0.0001
Cytoplasm 0.014 0.0094 0.0054 0.0134 0.0008 0.0111 0.0262 0.0229 0.0164 0.0062 0.0305 0.0052 0.0079 0.003 0.0145 0.0067 0.0049 0.0094
Cytoplasmic Foci 0.0919 0.1017 0.1137 0.0739 0.1076 0.0872 0.1355 0.1299 0.1547 0.1107 0.1228 0.1117 0.1273 0.1331 0.1164 0.1377 0.13 0.092
Eisosomes 0.0001 0 0.0001 0.0002 0 0 0.0003 0.0001 0.0002 0.0004 0.0001 0 0.0002 0 0.0003 0.0003 0.0005 0.0001
Endoplasmic Reticulum 0.0023 0.0015 0.0014 0.0104 0.0004 0.0007 0.0033 0.0032 0.0037 0.003 0.009 0.002 0.0075 0.0127 0.0116 0.0013 0.0088 0.0029
Endosome 0.099 0.1289 0.0865 0.1036 0.024 0.0572 0.0965 0.1161 0.1041 0.1197 0.1417 0.0784 0.1464 0.1396 0.1125 0.0845 0.1725 0.0836
Golgi 0.0339 0.0329 0.0306 0.0463 0.021 0.0222 0.0435 0.0305 0.035 0.0542 0.0409 0.0222 0.0363 0.0509 0.0533 0.0598 0.0473 0.0358
Lipid Particles 0.6489 0.5898 0.6215 0.3713 0.7309 0.6941 0.5022 0.5925 0.4636 0.4839 0.5148 0.6681 0.5385 0.5439 0.4816 0.4736 0.4322 0.6505
Mitochondria 0.0053 0.0022 0.0077 0.0141 0.0008 0.0054 0.0191 0.01 0.0221 0.0147 0.0073 0.012 0.0052 0.0015 0.013 0.021 0.0768 0.008
None 0.0126 0.0018 0.007 0.0617 0.0005 0.0109 0.022 0.0269 0.0724 0.017 0.0111 0.0039 0.0081 0.0198 0.0365 0.0467 0.0009 0.0223
Nuclear Periphery 0.0058 0.0016 0.0047 0.0247 0.0159 0.0075 0.0091 0.0033 0.0053 0.0015 0.0106 0.0078 0.0124 0.0089 0.0046 0.0015 0.0039 0.0086
Nucleolus 0.002 0.0019 0.0023 0.0054 0.0003 0.005 0.0028 0.0008 0.0008 0.0029 0.0018 0.0008 0.0096 0.0012 0.0007 0.0003 0.0006 0.0018
Nucleus 0.0016 0.002 0.0012 0.0057 0.0001 0.0022 0.0042 0.0008 0.0007 0.0006 0.0013 0.0004 0.0067 0.0005 0.001 0.0003 0.0007 0.0024
Peroxisomes 0.0613 0.0821 0.0997 0.2368 0.0804 0.0721 0.0628 0.0382 0.0867 0.1036 0.0597 0.0754 0.0545 0.0654 0.1014 0.1121 0.0557 0.0645
Punctate Nuclear 0.0093 0.0197 0.0046 0.0099 0.0153 0.0172 0.0209 0.0051 0.009 0.0033 0.029 0.0064 0.0101 0.0101 0.0047 0.003 0.0055 0.0116
Vacuole 0.0039 0.0099 0.0029 0.0086 0.0005 0.0025 0.0067 0.0063 0.0043 0.0035 0.004 0.0028 0.0054 0.0061 0.0053 0.0013 0.0068 0.0023
Vacuole Periphery 0.0014 0.0013 0.001 0.0044 0.0008 0.0015 0.0025 0.0017 0.0018 0.0014 0.0032 0.0017 0.0022 0.0018 0.0017 0.001 0.0024 0.0013

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 66.671 70.5264 37.5918 44.624 82.8401 47.7544 70.3048 57.2801 54.138 68.9417
Translational Efficiency 0.7489 0.7914 0.7862 0.6171 0.5972 0.8099 0.6504 0.6 0.4872 0.5372

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1387 751 3078 1557 1708 1888 267 178 3095 2639 3345 1735

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 742.38 580.58 962.67 928.67 713.58 802.29 1095.27 1018.86 726.49 739.20 973.25 937.92
Standard Deviation 83.37 97.57 124.41 123.35 85.58 107.18 119.38 144.71 85.80 144.69 129.11 128.65
Intensity Change Log 2 — -0.354663 0.374883 0.323008 — 0.169049 0.618139 0.513809 — -0.074305 0.499230 0.419675

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.003541 0.006019 0.061670 0.031964 0.002722 0.005046 0.039315 0.048312 0.003089 0.005323 0.059885 0.033641
Bud Neck 0.005879 0.005407 0.000629 0.002509 0.005346 0.005383 0.006009 0.003516 0.005585 0.005390 0.001059 0.002612
Bud Site 0.002025 0.003057 0.002756 0.010967 0.004413 0.004796 0.015787 0.044926 0.003343 0.004301 0.003796 0.014451
Cell Periphery 0.000247 0.000293 0.000076 0.000100 0.000192 0.000158 0.000361 0.000210 0.000217 0.000197 0.000099 0.000111
Cytoplasm 0.135551* 0.032010 0.001101 0.006716 0.063963 0.067914 0.016975 0.027316 0.096045 0.057696 0.002368 0.008829
Cytoplasmic Foci 0.225688* 0.229599* 0.208569* 0.315569* 0.193209* 0.238028* 0.197799* 0.233123* 0.207764* 0.235630* 0.207709* 0.307111*
Eisosomes 0.000725 0.000325 0.000135 0.000091 0.000410 0.000338 0.000192 0.000171 0.000551 0.000334 0.000139 0.000099
Endoplasmic Reticulum 0.014958 0.003731 0.002437 0.001692 0.008796 0.010998 0.036049 0.003026 0.011558 0.008930 0.005120 0.001829
Endosome 0.045235 0.149736 0.158975 0.261306* 0.061645 0.108309 0.165874 0.183126 0.054291 0.120098 0.159526 0.253285*
Golgi 0.062669 0.130938* 0.055428 0.080699 0.087720 0.089303 0.031972 0.045065 0.076494 0.101151 0.053556 0.077043
Lipid Particles 0.332623* 0.267238* 0.413626* 0.135737* 0.461531* 0.310949* 0.216025* 0.070425 0.403762* 0.298510* 0.397854* 0.129037*
Mitochondria 0.009834 0.024478 0.001531 0.005409 0.014612 0.018493 0.001206 0.006110 0.012471 0.020196 0.001505 0.005481
Mitotic Spindle 0.003528 0.007637 0.024460 0.064526 0.005674 0.024628 0.093599 0.140334* 0.004713 0.019793 0.029979 0.072303
None 0.001730 0.016364 0.000014 0.000075 0.001112 0.000858 0.000107 0.000169 0.001389 0.005270 0.000021 0.000084
Nuclear Periphery 0.001948 0.001944 0.000195 0.000614 0.001721 0.002374 0.002394 0.000583 0.001823 0.002252 0.000371 0.000611
Nuclear Periphery Foci 0.008171 0.013982 0.003391 0.009139 0.007778 0.011789 0.061086 0.015673 0.007954 0.012413 0.007997 0.009810
Nucleolus 0.001509 0.011177 0.000023 0.000707 0.001571 0.001136 0.000747 0.000113 0.001543 0.003994 0.000081 0.000646
Nucleus 0.080037 0.021764 0.000053 0.001766 0.021969 0.024269 0.011847 0.003739 0.047992 0.023556 0.000994 0.001969
Peroxisomes 0.046880 0.060916 0.060839 0.050313 0.042860 0.049623 0.048669 0.104367* 0.044661 0.052836 0.059867 0.055858
Vacuole 0.016129 0.010138 0.003673 0.016574 0.011152 0.023460 0.052736 0.065434 0.013382 0.019669 0.007590 0.021587
Vacuole Periphery 0.001090 0.003246 0.000418 0.003527 0.001605 0.002147 0.001252 0.004263 0.001375 0.002460 0.000484 0.003602

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.95 -19.82 -9.00 -7.90 7.22 -2.60 -5.06 -3.75 -3.51 -0.05 -3.09 -20.70 -9.92 -9.07 6.95
Bud Neck 0.35 7.32 3.92 1.85 -6.04 -0.04 -1.07 0.70 0.82 1.65 0.27 8.33 4.27 3.75 -5.41
Bud Site -2.05 -2.24 -6.46 -5.70 -5.89 -0.30 -4.91 -3.66 -3.64 -2.19 -1.22 -1.12 -6.37 -6.02 -6.19
Cell Periphery -0.80 7.54 6.04 3.85 -2.47 1.35 -3.41 -0.08 -0.50 2.05 0.93 6.79 5.36 4.45 -0.71
Cytoplasm 16.45 24.45 22.20 6.86 -5.04 -0.92 8.40 2.65 3.15 -2.14 9.27 29.34 23.61 15.10 -5.65
Cytoplasmic Foci -0.66 3.12 -10.46 -8.54 -15.43 -6.66 0.93 -0.42 2.46 -0.97 -5.27 0.85 -13.86 -9.48 -14.81
Eisosomes 2.50 3.98 4.27 3.88 6.24 1.79 6.21 6.12 4.34 1.23 2.96 6.01 6.62 8.63 5.84
Endoplasmic Reticulum 6.98 8.15 8.82 2.33 1.42 -1.55 -4.67 4.09 5.28 5.48 2.21 5.83 10.71 7.74 4.62
Endosome -14.57 -28.14* -35.17* -12.78 -15.16 -10.41 -9.13 -6.34 -3.08 0.09 -17.66 -29.14 -34.78 -20.78 -14.33
Golgi -10.04 1.84 -3.97 7.69 -7.26 -0.24 11.79 6.96 7.18 -0.85 -6.25 8.04 0.48 6.47 -6.97
Lipid Particles 4.78 -7.87 19.21 10.95 34.12* 13.50 15.72 31.12 20.57 8.52 12.16 0.57 34.71 21.79 35.18*
Mitochondria -5.20 8.85 3.18 6.68 -3.26 -1.94 9.01 2.80 3.90 -1.13 -4.94 12.04 5.27 9.01 -3.44
Mitotic Spindle -2.34 -10.14 -14.12 -12.85 -8.93 -8.34 -7.08 -6.60 -5.63 -1.87 -8.93 -12.05 -15.38 -11.54 -9.05
None -4.36 7.98 7.66 4.92 -2.71 0.90 5.12 3.60 1.63 -1.59 -3.82 9.57 8.93 5.30 -3.26
Nuclear Periphery 0.02 8.98 4.22 1.98 -1.85 -1.52 -2.03 1.59 3.40 3.56 -1.29 6.98 3.82 4.33 -1.30
Nuclear Periphery Foci -2.98 5.01 -0.86 2.20 -4.49 -3.01 -6.95 -2.02 -1.15 5.18 -4.15 -0.70 -1.73 1.46 -1.12
Nucleolus -4.98 5.79 2.62 5.43 -3.02 1.38 1.89 4.29 6.35 2.02 -4.13 7.20 3.22 5.58 -2.80
Nucleus 10.10 17.32 16.67 5.93 -3.06 -0.87 2.23 0.80 1.22 -0.61 8.43 19.67 17.64 11.19 -3.01
Peroxisomes -2.67 -3.65 -0.85 1.96 2.70 -1.95 -0.58 -3.12 -2.60 -2.56 -2.84 -4.93 -2.60 -0.26 1.60
Vacuole 3.91 10.81 -2.51 -5.21 -9.11 -6.65 -6.92 -7.34 -6.65 -3.80 -4.57 3.91 -7.50 -4.96 -8.98
Vacuole Periphery -4.10 5.90 -3.25 -0.31 -4.19 -1.84 0.70 -1.16 -0.81 -1.37 -4.52 6.06 -3.06 -1.46 -4.43
Standard name
Human Ortholog
Description Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts
Localization
Cell Percentages ER (12%)
Cell Cycle Regulation No
Subcompartmental Group ER-2

Env9

Env9


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Env9-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available