Standard name | |
---|---|
Human Ortholog | |
Description | Delta(24)-sterol C-methyltransferase; converts zymosterol to fecosterol in the ergosterol biosynthetic pathway by methylating position C-24; localized to lipid particles, the plasma membrane-associated endoplasmic reticulum, and the mitochondrial outer membrane |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 5 | 2 | 12 | 16 | 32 | 22 | 13 | 7 | 2 | 10 | 3 | 3 | 5 | 1 | 2 | 1 | 1 | 0 | 34 | 27 | 70 | 36 | 32 | 31 |
Bud | 1 | 0 | 4 | 6 | 11 | 6 | 4 | 5 | 5 | 3 | 1 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 2 | 3 | 1 |
Bud Neck | 0 | 0 | 5 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 2 | 9 | 2 | 1 | 0 |
Bud Site | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1 | 3 | 1 | 3 | 3 | 6 | 7 | 17 | 2 | 12 | 5 | 2 | 3 | 1 | 3 | 10 | 4 | 13 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 3 | 1 | 2 | 3 | 19 | 6 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 5 | 8 | 3 | 0 | 3 | 1 | 1 |
Endosome | 0 | 0 | 2 | 4 | 3 | 0 | 2 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 2 | 2 | 1 | 18 | 11 | 28 | 25 | 17 | 22 |
Golgi | 40 | 24 | 32 | 76 | 127 | 23 | 27 | 58 | 4 | 48 | 7 | 10 | 25 | 15 | 18 | 5 | 5 | 10 | 98 | 78 | 140 | 124 | 124 | 137 |
Mitochondria | 121 | 115 | 272 | 186 | 230 | 529 | 680 | 665 | 575 | 920 | 799 | 952 | 172 | 123 | 196 | 19 | 9 | 14 | 9 | 20 | 28 | 13 | 17 | 13 |
Nucleus | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 0 | 1 | 3 | 4 | 4 | 2 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 2 | 6 | 5 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Nucleolus | 104 | 88 | 168 | 124 | 92 | 181 | 285 | 295 | 226 | 369 | 321 | 410 | 249 | 187 | 306 | 190 | 172 | 173 | 22 | 30 | 33 | 32 | 22 | 21 |
Peroxisomes | 0 | 3 | 22 | 10 | 3 | 0 | 5 | 2 | 2 | 2 | 1 | 0 | 5 | 1 | 3 | 4 | 3 | 1 | 23 | 33 | 154 | 64 | 59 | 90 |
SpindlePole | 0 | 0 | 5 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 6 | 1 | 6 | 3 | 5 | 2 | 5 | 6 | 25 | 12 | 16 | 10 |
Vac/Vac Membrane | 1 | 3 | 3 | 1 | 14 | 4 | 8 | 26 | 13 | 42 | 20 | 21 | 2 | 6 | 5 | 2 | 3 | 6 | 2 | 6 | 3 | 11 | 4 | 6 |
Unique Cell Count | 201 | 177 | 393 | 314 | 391 | 596 | 783 | 821 | 616 | 1063 | 858 | 1041 | 341 | 240 | 390 | 217 | 193 | 207 | 231 | 230 | 502 | 333 | 308 | 341 |
Labelled Cell Count | 277 | 240 | 531 | 433 | 535 | 779 | 1035 | 1080 | 838 | 1416 | 1166 | 1407 | 472 | 337 | 540 | 237 | 206 | 227 | 231 | 230 | 502 | 333 | 308 | 341 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 50.1 | 46.6 | 31.7 | 34.7 | 34.3 | 33.2 | 36.5 | 39.2 | 38.0 | 41.5 | 37.9 | 40.4 | 48.0 | 50.7 | 50.3 | 49.4 | 49.5 | 54.5 | 44.3 | 44.1 | 47.8 |
Std Deviation (1e-4) | 12.3 | 14.3 | 8.5 | 8.7 | 8.3 | 8.1 | 9.1 | 12.7 | 10.3 | 12.8 | 11.9 | 12.4 | 14.0 | 16.1 | 15.2 | 21.5 | 19.9 | 24.1 | 13.1 | 14.3 | 15.7 |
Intensity Change (Log2) | – | – | – | 0.13 | 0.11 | 0.07 | 0.2 | 0.3 | 0.26 | 0.39 | 0.26 | 0.35 | 0.6 | 0.68 | 0.66 | 0.64 | 0.64 | 0.78 | 0.48 | 0.48 | 0.59 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 1.4 | 3.1 | 0.5 | -1.6 | -2.9 | -3.6 | -2.9 | 0 | 0 | -1.4 | 0 | -2.7 | 0 | 0 | 0 |
Bud | 0 | 1.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 3.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 5.9 | 8.5 | -2.9 | -3.5 | -0.7 | -6.3 | -2.7 | -6.9 | -7.2 | -0.4 | -0.9 | -2.0 | -2.9 | -2.6 | -1.5 |
Mitochondria | -2.8 | -3.0 | 7.7 | 7.3 | 4.6 | 10.2 | 7.6 | 11.2 | 10.6 | -5.2 | -4.5 | -5.4 | -14.3 | -14.7 | -14.6 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -0.9 | -5.7 | -4.0 | -2.1 | -2.3 | -1.9 | -2.8 | -1.8 | -1.2 | 8.3 | 8.7 | 10.2 | 10.8 | 10.7 | 9.6 |
Peroxisomes | -1.5 | -3.8 | -5.8 | -5.4 | -6.3 | -5.4 | -7.2 | -6.7 | -7.7 | -3.0 | -3.4 | -3.8 | -2.2 | -2.3 | -3.1 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.5 | 0 | 0.3 | 0 | 0 | 0 |
Vacuole | 0 | 2.7 | 0 | 0 | 2.6 | 1.7 | 3.1 | 1.9 | 1.7 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 43.6966 | 53.259 | 50.9574 | 48.9538 | 37.5117 | 50.0288 | 58.435 | 61.4317 | 60.4943 | 53.064 | 56.2078 | 58.5229 | 54.7839 | 62.915 | 55.6739 | 50.4602 | 48.9266 | 56.6768 |
Actin | 0.0016 | 0.004 | 0.0047 | 0.0531 | 0.0016 | 0.0035 | 0.002 | 0.0055 | 0.0032 | 0.0082 | 0.0018 | 0.0033 | 0.0056 | 0.0007 | 0.0038 | 0.0007 | 0.0117 | 0.002 |
Bud | 0.003 | 0.0002 | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 |
Bud Neck | 0.0005 | 0.0012 | 0.0008 | 0.0019 | 0.0002 | 0.0004 | 0.0009 | 0.0004 | 0.0004 | 0.0021 | 0.0001 | 0.0002 | 0.0002 | 0.0006 | 0.0005 | 0.0052 | 0.0005 | 0.0006 |
Bud Periphery | 0.0011 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0.0001 | 0.0001 | 0.0003 | 0.0003 | 0.0001 | 0 |
Bud Site | 0.0094 | 0.0038 | 0.0009 | 0.0021 | 0.0002 | 0.0003 | 0.0007 | 0.0005 | 0.0005 | 0.0014 | 0.0001 | 0.0001 | 0.0004 | 0.0016 | 0.0006 | 0.0014 | 0.0003 | 0.0002 |
Cell Periphery | 0.0011 | 0.0033 | 0.0012 | 0.0013 | 0.0008 | 0.0027 | 0.0011 | 0.001 | 0.0009 | 0.0024 | 0.0005 | 0.0006 | 0.001 | 0.0011 | 0.0011 | 0.0007 | 0.0006 | 0.0008 |
Cytoplasm | 0.0001 | 0 | 0.0007 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0 | 0.002 | 0 | 0 | 0.0008 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Cytoplasmic Foci | 0.0409 | 0.0488 | 0.0337 | 0.0289 | 0.1018 | 0.0244 | 0.0484 | 0.0415 | 0.0355 | 0.032 | 0.0471 | 0.0291 | 0.0293 | 0.0544 | 0.0383 | 0.0279 | 0.0743 | 0.0422 |
Eisosomes | 0.0005 | 0.0012 | 0.0007 | 0.0009 | 0.0004 | 0.0008 | 0.0006 | 0.0004 | 0.0004 | 0.0007 | 0.0005 | 0.0004 | 0.0012 | 0.0007 | 0.001 | 0.0006 | 0.0006 | 0.0007 |
Endoplasmic Reticulum | 0 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0007 | 0 | 0 | 0.0003 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 |
Endosome | 0.0018 | 0.0019 | 0.0034 | 0.002 | 0.0044 | 0.0022 | 0.0019 | 0.0015 | 0.0013 | 0.0035 | 0.0006 | 0.0008 | 0.0056 | 0.0009 | 0.002 | 0.0055 | 0.0023 | 0.0004 |
Golgi | 0.0051 | 0.0217 | 0.0152 | 0.0191 | 0.0133 | 0.0175 | 0.0059 | 0.0132 | 0.0229 | 0.0086 | 0.0075 | 0.0098 | 0.0073 | 0.0052 | 0.0139 | 0.0081 | 0.0053 | 0.0049 |
Lipid Particles | 0.2412 | 0.3898 | 0.3805 | 0.4701 | 0.3743 | 0.4439 | 0.4625 | 0.5517 | 0.507 | 0.6223 | 0.5913 | 0.6824 | 0.3857 | 0.4184 | 0.3974 | 0.5859 | 0.343 | 0.5596 |
Mitochondria | 0.2431 | 0.112 | 0.2656 | 0.2223 | 0.3722 | 0.2503 | 0.2101 | 0.1327 | 0.2128 | 0.1373 | 0.206 | 0.1515 | 0.2369 | 0.2112 | 0.2999 | 0.2508 | 0.3296 | 0.2249 |
None | 0.0003 | 0.0001 | 0.0005 | 0.0001 | 0.0008 | 0.0001 | 0.0006 | 0 | 0 | 0.0035 | 0.0002 | 0 | 0.0007 | 0 | 0.0002 | 0.0001 | 0.0003 | 0.0004 |
Nuclear Periphery | 0.002 | 0.0019 | 0.0009 | 0.0002 | 0.0009 | 0.0007 | 0.0002 | 0.0001 | 0.0001 | 0.0013 | 0.0001 | 0.0002 | 0.002 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0001 |
Nucleolus | 0.3887 | 0.3321 | 0.2041 | 0.1234 | 0.0428 | 0.1645 | 0.1904 | 0.1796 | 0.1467 | 0.0757 | 0.0587 | 0.0671 | 0.2457 | 0.2523 | 0.1621 | 0.0539 | 0.1236 | 0.114 |
Nucleus | 0.0128 | 0.0126 | 0.0047 | 0.0032 | 0.002 | 0.0059 | 0.0035 | 0.0038 | 0.0029 | 0.0032 | 0.0008 | 0.0011 | 0.0085 | 0.0047 | 0.0035 | 0.0017 | 0.0029 | 0.0022 |
Peroxisomes | 0.0299 | 0.0241 | 0.0508 | 0.0349 | 0.0539 | 0.0345 | 0.0515 | 0.0434 | 0.051 | 0.0538 | 0.0703 | 0.0347 | 0.0389 | 0.0345 | 0.0564 | 0.0419 | 0.0964 | 0.0334 |
Punctate Nuclear | 0.0017 | 0.0005 | 0.0117 | 0.0004 | 0.0127 | 0.0007 | 0.0014 | 0.0003 | 0.0005 | 0.0018 | 0.0005 | 0.0003 | 0.0104 | 0.0005 | 0.0004 | 0.0002 | 0.0013 | 0.001 |
Vacuole | 0.0024 | 0.0092 | 0.0028 | 0.004 | 0.0027 | 0.0052 | 0.0024 | 0.0029 | 0.0018 | 0.0093 | 0.0013 | 0.0012 | 0.0019 | 0.0025 | 0.003 | 0.0054 | 0.0009 | 0.0012 |
Vacuole Periphery | 0.0128 | 0.0314 | 0.0165 | 0.0318 | 0.0144 | 0.0423 | 0.0156 | 0.0215 | 0.0121 | 0.0298 | 0.0124 | 0.0172 | 0.0173 | 0.0101 | 0.0151 | 0.0092 | 0.006 | 0.0114 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 286.866 | 334.7857 | 303.7016 | 305.095 | 304.8744 | 218.2612 | 317.7239 | 323.4162 | 336.2687 | 304.9227 |
Translational Efficiency | 2.0062 | 1.827 | 1.6416 | 1.784 | 1.5674 | 2.3148 | 1.7377 | 1.3256 | 1.325 | 1.588 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1378 | 1632 | 127 | 1335 | 1734 | 1751 | 1773 | 1446 | 3112 | 3383 | 1900 | 2781 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 876.29 | 1196.64 | 1737.25 | 1121.50 | 1117.72 | 1038.04 | 1445.27 | 1148.80 | 1010.81 | 1114.55 | 1464.79 | 1135.69 |
Standard Deviation | 145.21 | 349.61 | 267.28 | 183.80 | 235.63 | 185.63 | 258.78 | 199.37 | 233.78 | 288.24 | 269.42 | 192.54 |
Intensity Change Log 2 | — | 0.449509 | 0.987325 | 0.355949 | — | -0.106697 | 0.370780 | 0.039569 | — | 0.164396 | 0.674497 | 0.187210 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001337 | 0.005322 | 0.016900 | 0.023440 | 0.003510 | 0.003140 | 0.010734 | 0.018416 | 0.002548 | 0.004193 | 0.011146 | 0.020827 |
Bud Neck | 0.005982 | 0.004816 | 0.017634 | 0.006096 | 0.003289 | 0.004030 | 0.004432 | 0.005313 | 0.004481 | 0.004409 | 0.005315 | 0.005688 |
Bud Site | 0.043474 | 0.091772 | 0.030354 | 0.114342* | 0.074612 | 0.056526 | 0.079446 | 0.110070* | 0.060824 | 0.073529 | 0.076165 | 0.112121* |
Cell Periphery | 0.000397 | 0.000988 | 0.000675 | 0.000591 | 0.000572 | 0.000474 | 0.000869 | 0.001064 | 0.000494 | 0.000722 | 0.000856 | 0.000837 |
Cytoplasm | 0.044558 | 0.027121 | 0.001923 | 0.030667 | 0.025031 | 0.021416 | 0.017673 | 0.025190 | 0.033678 | 0.024168 | 0.016620 | 0.027819 |
Cytoplasmic Foci | 0.152879 | 0.066690 | 0.091754 | 0.097580 | 0.082211 | 0.097967 | 0.038432 | 0.087867 | 0.113503 | 0.082879 | 0.041996 | 0.092529 |
Eisosomes | 0.000098 | 0.000088 | 0.000136 | 0.000123 | 0.000071 | 0.000077 | 0.000095 | 0.000142 | 0.000083 | 0.000082 | 0.000098 | 0.000133 |
Endoplasmic Reticulum | 0.003803 | 0.001913 | 0.001702 | 0.002654 | 0.002470 | 0.000929 | 0.001530 | 0.002574 | 0.003060 | 0.001404 | 0.001541 | 0.002612 |
Endosome | 0.100879 | 0.080816 | 0.088837 | 0.073101 | 0.089935 | 0.107349 | 0.048772 | 0.078650 | 0.094781 | 0.094549 | 0.051450 | 0.075987 |
Golgi | 0.162881* | 0.188903* | 0.251096* | 0.192414* | 0.188564* | 0.209358* | 0.177607* | 0.214370* | 0.177191* | 0.199491* | 0.182519* | 0.203830* |
Lipid Particles | 0.003647 | 0.002303 | 0.009729 | 0.001872 | 0.001018 | 0.001259 | 0.000796 | 0.001372 | 0.002182 | 0.001763 | 0.001393 | 0.001612 |
Mitochondria | 0.303500* | 0.341518* | 0.174470* | 0.186967* | 0.369419* | 0.341928* | 0.443164* | 0.273087* | 0.340230* | 0.341730* | 0.425204* | 0.231746* |
Mitotic Spindle | 0.036497 | 0.034448 | 0.029224 | 0.172867* | 0.037903 | 0.041417 | 0.058008 | 0.086194 | 0.037280 | 0.038055 | 0.056084 | 0.127801* |
None | 0.000986 | 0.002567 | 0.000632 | 0.000309 | 0.000555 | 0.000465 | 0.000781 | 0.000490 | 0.000746 | 0.001479 | 0.000771 | 0.000403 |
Nuclear Periphery | 0.000485 | 0.000262 | 0.006414 | 0.000241 | 0.000184 | 0.000171 | 0.000325 | 0.000222 | 0.000318 | 0.000215 | 0.000732 | 0.000231 |
Nuclear Periphery Foci | 0.001322 | 0.001565 | 0.008575 | 0.000926 | 0.000651 | 0.000443 | 0.000631 | 0.000705 | 0.000948 | 0.000984 | 0.001162 | 0.000811 |
Nucleolus | 0.001196 | 0.000475 | 0.000893 | 0.000166 | 0.000106 | 0.000289 | 0.000130 | 0.000143 | 0.000589 | 0.000379 | 0.000181 | 0.000154 |
Nucleus | 0.003461 | 0.001908 | 0.000344 | 0.000411 | 0.000310 | 0.001169 | 0.000179 | 0.000338 | 0.001705 | 0.001526 | 0.000190 | 0.000373 |
Peroxisomes | 0.014770 | 0.011901 | 0.011047 | 0.022689 | 0.009583 | 0.013883 | 0.012128 | 0.026700 | 0.011880 | 0.012927 | 0.012056 | 0.024774 |
Vacuole | 0.057425 | 0.067781 | 0.142093 | 0.045775 | 0.049185 | 0.049356 | 0.046492 | 0.037644 | 0.052834 | 0.058245 | 0.052882 | 0.041547 |
Vacuole Periphery | 0.060422 | 0.066841 | 0.115568* | 0.026770 | 0.060821 | 0.048353 | 0.057776 | 0.029450 | 0.060644 | 0.057272 | 0.061639 | 0.028163 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -6.60 | -2.28 | -10.46 | -8.30 | -0.89 | 0.58 | -5.72 | -7.60 | -7.74 | -3.39 | -3.73 | -7.00 | -12.68 | -11.33 | -5.15 |
Bud Neck | 1.40 | -3.66 | -0.58 | -2.50 | 3.54 | -1.63 | -2.14 | -3.43 | -2.13 | -1.28 | 0.17 | -1.46 | -2.79 | -3.26 | -0.88 |
Bud Site | -10.16 | 1.69 | -13.61 | -3.95 | -10.05 | 4.02 | -1.23 | -6.07 | -9.64 | -5.05 | -3.84 | -4.11 | -12.90 | -9.50 | -7.62 |
Cell Periphery | -5.52 | -2.54 | -2.10 | 4.62 | 1.01 | 1.68 | -4.53 | -5.13 | -6.45 | -1.98 | -3.87 | -5.91 | -5.21 | -1.69 | 0.40 |
Cytoplasm | 4.47 | 13.53 | 2.98 | -1.84 | -13.96 | 1.43 | 3.38 | -0.44 | -1.81 | -3.68 | 4.26 | 8.20 | 1.88 | -2.57 | -6.85 |
Cytoplasmic Foci | 17.48 | 5.11 | 10.28 | -7.33 | -0.43 | -4.10 | 14.09 | -1.47 | 2.31 | -13.88 | 9.81 | 23.52 | 6.25 | -3.19 | -17.38 |
Eisosomes | 0.62 | -1.57 | -1.31 | -5.58 | 0.80 | -1.32 | -4.60 | -9.19 | -8.29 | -5.60 | 0.14 | -1.65 | -5.47 | -9.98 | -5.60 |
Endoplasmic Reticulum | 2.57 | 2.72 | 1.39 | -1.48 | -1.74 | 3.79 | 2.04 | -0.15 | -3.37 | -1.95 | 4.18 | 3.44 | 0.91 | -3.49 | -2.69 |
Endosome | 4.66 | 1.49 | 6.67 | 2.39 | 2.18 | -4.29 | 12.91 | 3.08 | 7.32 | -9.63 | 0.09 | 16.04 | 6.86 | 7.08 | -9.73 |
Golgi | -3.45 | -3.58 | -2.98 | 0.21 | 2.56 | -2.73 | 1.53 | -2.88 | -0.38 | -4.22 | -4.14 | -0.74 | -3.96 | -0.14 | -2.62 |
Lipid Particles | 2.41 | -2.28 | 2.92 | 0.52 | 2.90 | -0.82 | 0.93 | -1.44 | -0.51 | -2.51 | 1.30 | 2.60 | 1.45 | 0.11 | -1.18 |
Mitochondria | -3.14 | 5.33 | 10.79 | 13.89 | -0.32 | 2.45 | -6.01 | 8.41 | 6.16 | 14.06* | -0.14 | -8.11 | 13.62 | 13.84 | 19.19* |
Mitotic Spindle | 0.58 | 0.93 | -17.99 | -18.60 | -14.78 | -1.06 | -5.62 | -9.20 | -8.44 | -5.09 | -0.33 | -5.98 | -19.60 | -19.55 | -14.15 |
None | -2.65 | 2.47 | 5.25 | 3.87 | 4.63 | 1.76 | -6.50 | 0.71 | -0.14 | 3.07 | -2.55 | -0.35 | 4.53 | 3.77 | 6.90 |
Nuclear Periphery | 1.92 | -2.89 | 2.17 | 0.17 | 3.01 | 0.29 | -3.19 | -0.72 | -0.93 | 1.69 | 1.78 | -2.73 | 1.39 | -0.58 | 3.36 |
Nuclear Periphery Foci | -0.42 | -1.70 | 0.63 | 1.20 | 1.78 | 0.73 | 0.18 | -0.43 | -1.07 | -0.63 | -0.27 | -0.57 | 0.36 | 0.67 | 0.94 |
Nucleolus | 2.62 | 0.88 | 3.89 | 3.01 | 3.16 | -2.09 | -1.03 | -1.17 | 1.35 | -0.73 | 1.44 | 3.48 | 3.46 | 2.95 | 0.15 |
Nucleus | 2.46 | 6.86 | 6.24 | 2.99 | -2.26 | -1.99 | 2.57 | -0.94 | 1.79 | -2.52 | 0.36 | 7.39 | 5.74 | 3.32 | -4.59 |
Peroxisomes | 1.54 | 1.38 | -3.27 | -4.86 | -3.95 | -3.00 | -1.76 | -7.48 | -5.34 | -6.07 | -0.92 | -0.12 | -7.86 | -7.21 | -7.11 |
Vacuole | -2.77 | -5.75 | 1.68 | 4.33 | 6.18 | -0.04 | 0.72 | 3.46 | 3.51 | 2.78 | -2.30 | -0.22 | 3.40 | 5.57 | 3.12 |
Vacuole Periphery | -1.59 | -4.50 | 10.11 | 11.96 | 7.42 | 3.76 | 0.86 | 9.81 | 6.93 | 8.94 | 1.30 | -0.28 | 14.09 | 13.53 | 11.75 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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