Standard name | |
---|---|
Human Ortholog | |
Description | Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C16:0-C18:0 chain lengths; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 32 | 12 | 16 | 15 | 41 | 22 | 48 | 32 | 34 | 22 | 19 | 23 | 29 | 41 | 50 | 71 | 44 | 34 | 15 | 14 | 24 | 11 | 21 | 20 |
Bud | 15 | 11 | 2 | 6 | 13 | 15 | 26 | 22 | 27 | 23 | 35 | 27 | 18 | 10 | 15 | 8 | 11 | 10 | 3 | 14 | 16 | 8 | 11 | 15 |
Bud Neck | 0 | 2 | 13 | 9 | 18 | 10 | 20 | 16 | 11 | 13 | 4 | 8 | 0 | 1 | 0 | 0 | 1 | 0 | 9 | 24 | 24 | 12 | 12 | 18 |
Bud Site | 1 | 0 | 0 | 0 | 2 | 1 | 1 | 0 | 2 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 107 | 86 | 135 | 124 | 141 | 99 | 92 | 40 | 74 | 54 | 45 | 51 | 124 | 180 | 214 | 268 | 161 | 190 | 129 | 93 | 176 | 103 | 181 | 229 |
Cytoplasm | 141 | 165 | 298 | 290 | 316 | 405 | 482 | 426 | 406 | 382 | 360 | 369 | 122 | 229 | 286 | 135 | 78 | 75 | 46 | 73 | 105 | 32 | 19 | 51 |
Endoplasmic Reticulum | 4 | 6 | 6 | 1 | 3 | 7 | 5 | 8 | 4 | 4 | 0 | 4 | 1 | 1 | 4 | 20 | 3 | 19 | 23 | 18 | 10 | 10 | 12 | 22 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 2 | 2 | 3 | 0 | 0 | 1 |
Golgi | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 2 | 1 | 0 | 2 | 2 | 1 | 0 |
Mitochondria | 3 | 1 | 1 | 11 | 9 | 44 | 63 | 37 | 99 | 83 | 108 | 95 | 0 | 1 | 0 | 28 | 9 | 10 | 9 | 9 | 30 | 9 | 16 | 14 |
Nucleus | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 4 | 1 | 1 | 7 | 1 | 0 | 1 | 1 | 3 | 3 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 0 | 1 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 3 | 0 | 3 | 2 | 7 | 6 | 5 | 9 | 4 | 12 | 2 | 12 | 0 | 1 | 3 | 8 | 3 | 10 | 0 | 0 | 2 | 1 | 1 | 2 |
Vac/Vac Membrane | 0 | 1 | 1 | 0 | 0 | 2 | 4 | 11 | 2 | 8 | 13 | 10 | 1 | 0 | 0 | 8 | 6 | 1 | 4 | 7 | 15 | 4 | 6 | 8 |
Unique Cell Count | 232 | 219 | 376 | 363 | 442 | 497 | 604 | 529 | 524 | 491 | 488 | 499 | 209 | 352 | 434 | 428 | 266 | 277 | 251 | 264 | 424 | 201 | 288 | 387 |
Labelled Cell Count | 308 | 285 | 476 | 458 | 552 | 611 | 749 | 607 | 664 | 605 | 596 | 601 | 295 | 467 | 577 | 551 | 322 | 356 | 251 | 264 | 424 | 201 | 288 | 387 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.9 | 6.4 | 6.1 | 5.7 | 6.1 | 4.8 | 4.9 | 5.2 | 4.3 | 4.5 | 4.3 | 4.8 | 7.0 | 7.0 | 7.2 | 10.8 | 10.6 | 10.7 | 6.2 | 7.0 | 6.8 |
Std Deviation (1e-4) | 2.4 | 2.2 | 1.5 | 1.7 | 1.5 | 1.3 | 1.3 | 1.3 | 1.2 | 1.4 | 1.4 | 1.3 | 2.1 | 2.3 | 2.3 | 2.2 | 2.2 | 2.1 | 2.1 | 2.3 | 2.1 |
Intensity Change (Log2) | – | – | – | -0.1 | 0.01 | -0.35 | -0.31 | -0.24 | -0.52 | -0.46 | -0.5 | -0.35 | 0.19 | 0.2 | 0.24 | 0.82 | 0.8 | 0.81 | 0.01 | 0.19 | 0.15 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.1 | 2.8 | 0 | 2.3 | 1.2 | 0 | 0 | 0 | 0 | 4.2 | 3.7 | 3.8 | 5.6 | 5.3 | 3.8 |
Bud | 0 | 2.6 | 0 | 3.4 | 3.3 | 0 | 0 | 0 | 0 | 5.2 | 2.4 | 2.9 | 0 | 3.2 | 2.9 |
Bud Neck | -0.8 | 0.5 | 0 | -0.1 | -0.4 | 0 | 0 | 0 | 0 | 0 | -3.1 | -3.9 | -3.9 | -2.6 | -3.1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.5 | -1.2 | 0 | -7.5 | -10.6 | 0 | 0 | 0 | 0 | 5.5 | 4.1 | 3.8 | 7.6 | 6.2 | 8.3 |
Cytoplasm | 0.2 | -2.6 | 0.8 | 0.2 | 0.5 | -0.6 | -0.5 | -1.9 | -1.8 | -5.4 | -4.3 | -4.2 | -13.5 | -12.7 | -13.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | -0.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.5 | 0 | 3.5 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3.0 | 0 | 0 | 6.3 | 5.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.8 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.3 | 0 | 2.5 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.9378 | 1.8938 | 1.0914 | 1.795 | 0.8741 | 1.2894 | 1.1638 | 2.019 | 1.2883 | 1.3462 | -0.1744 | 0.9986 | 0.9492 | 1.2072 | 0.3882 | 1.3135 | 0.9868 | 1.195 |
Actin | 0.0672 | 0.0142 | 0.0084 | 0.0589 | 0.0934 | 0.0182 | 0.0332 | 0.0059 | 0.0217 | 0.0292 | 0.0177 | 0.0257 | 0.0557 | 0.0292 | 0.0212 | 0.0039 | 0.0124 | 0.0043 |
Bud | 0.0012 | 0.0022 | 0.0015 | 0.0026 | 0.0094 | 0.003 | 0.0021 | 0.0014 | 0.0029 | 0.0009 | 0.0046 | 0.0011 | 0.0015 | 0.0005 | 0.0014 | 0.0018 | 0.0014 | 0.0005 |
Bud Neck | 0.0086 | 0.0067 | 0.0272 | 0.0318 | 0.0138 | 0.0366 | 0.0055 | 0.0089 | 0.0395 | 0.0107 | 0.0678 | 0.0499 | 0.005 | 0.0057 | 0.0223 | 0.0205 | 0.04 | 0.0681 |
Bud Periphery | 0.0083 | 0.0076 | 0.0141 | 0.0443 | 0.0421 | 0.0313 | 0.014 | 0.0038 | 0.0125 | 0.0069 | 0.0309 | 0.0128 | 0.0037 | 0.0016 | 0.0116 | 0.0166 | 0.0065 | 0.0063 |
Bud Site | 0.0318 | 0.1714 | 0.0731 | 0.0132 | 0.0129 | 0.0043 | 0.0275 | 0.1809 | 0.0711 | 0.0059 | 0.0211 | 0.0037 | 0.0255 | 0.105 | 0.0401 | 0.0037 | 0.0303 | 0.0067 |
Cell Periphery | 0.3241 | 0.2427 | 0.2602 | 0.4082 | 0.3326 | 0.3304 | 0.3763 | 0.3848 | 0.3435 | 0.3546 | 0.3098 | 0.3267 | 0.2392 | 0.1815 | 0.1897 | 0.3061 | 0.1096 | 0.2588 |
Cytoplasm | 0.3222 | 0.4093 | 0.3505 | 0.3451 | 0.3168 | 0.4063 | 0.2895 | 0.3038 | 0.3202 | 0.2626 | 0.3492 | 0.3551 | 0.3373 | 0.4677 | 0.3955 | 0.5025 | 0.4901 | 0.4269 |
Cytoplasmic Foci | 0.0151 | 0.0034 | 0.0141 | 0.0006 | 0.007 | 0.003 | 0.0139 | 0.0034 | 0.0095 | 0.0072 | 0.0069 | 0.0118 | 0.0189 | 0.0024 | 0.0053 | 0.0293 | 0.0226 | 0.0015 |
Eisosomes | 0.0085 | 0.0035 | 0.0047 | 0.0034 | 0.0023 | 0.0024 | 0.0038 | 0.0019 | 0.0033 | 0.0015 | 0.0005 | 0.0009 | 0.0013 | 0.001 | 0.0007 | 0.001 | 0.0028 | 0.0017 |
Endoplasmic Reticulum | 0.0097 | 0.0031 | 0.0042 | 0.0037 | 0.009 | 0.0067 | 0.0053 | 0.0027 | 0.0032 | 0.0056 | 0.0036 | 0.003 | 0.0117 | 0.0118 | 0.0081 | 0.0121 | 0.002 | 0.0039 |
Endosome | 0.0101 | 0.0005 | 0.0066 | 0.0017 | 0.0184 | 0.0042 | 0.0185 | 0.0023 | 0.0055 | 0.0308 | 0.0318 | 0.0066 | 0.004 | 0.0003 | 0.0023 | 0.0011 | 0.0004 | 0.0002 |
Golgi | 0.006 | 0.0012 | 0.0036 | 0.0053 | 0.015 | 0.0011 | 0.0138 | 0.001 | 0.0045 | 0.0297 | 0.0131 | 0.0063 | 0.0042 | 0.0007 | 0.0085 | 0.0007 | 0.0005 | 0.0003 |
Lipid Particles | 0.0062 | 0.0007 | 0.0054 | 0.0011 | 0.0067 | 0.0013 | 0.0163 | 0.0006 | 0.0025 | 0.0077 | 0.002 | 0.0061 | 0.0124 | 0.0015 | 0.0037 | 0.0041 | 0.0023 | 0.0004 |
Mitochondria | 0.003 | 0.0002 | 0.0045 | 0.0007 | 0.0154 | 0.0013 | 0.0114 | 0.0005 | 0.006 | 0.0581 | 0.0063 | 0.002 | 0.0008 | 0.0001 | 0.006 | 0.0005 | 0.0004 | 0.0001 |
None | 0.1596 | 0.1293 | 0.2046 | 0.0742 | 0.0772 | 0.1378 | 0.1552 | 0.0946 | 0.1389 | 0.1775 | 0.1266 | 0.1815 | 0.2644 | 0.1882 | 0.2709 | 0.0809 | 0.2724 | 0.218 |
Nuclear Periphery | 0.0025 | 0.0003 | 0.0005 | 0.0013 | 0.0071 | 0.0047 | 0.0013 | 0.0003 | 0.0004 | 0.0004 | 0.0006 | 0.0002 | 0.0009 | 0.0004 | 0.0009 | 0.0007 | 0.0002 | 0.0002 |
Nucleolus | 0.0007 | 0.0003 | 0.0026 | 0.0002 | 0.0008 | 0.0003 | 0.0005 | 0.0002 | 0.0005 | 0.0002 | 0.0002 | 0.0002 | 0.0007 | 0.0001 | 0.0001 | 0.0006 | 0.0011 | 0.0001 |
Nucleus | 0.0017 | 0.0006 | 0.0015 | 0.0005 | 0.0029 | 0.0011 | 0.0008 | 0.0005 | 0.0009 | 0.0004 | 0.0007 | 0.0004 | 0.0005 | 0.0004 | 0.0005 | 0.0009 | 0.0007 | 0.0003 |
Peroxisomes | 0.0044 | 0.0002 | 0.0075 | 0.0001 | 0.0041 | 0.0007 | 0.0055 | 0.0001 | 0.0052 | 0.0049 | 0.0004 | 0.0033 | 0.0081 | 0.0004 | 0.0065 | 0.0009 | 0.0002 | 0 |
Punctate Nuclear | 0.0049 | 0.0003 | 0.0016 | 0.0002 | 0.0032 | 0.0004 | 0.001 | 0.0002 | 0.0017 | 0.0002 | 0.0003 | 0.0003 | 0.0016 | 0.0002 | 0.0002 | 0.0041 | 0.0008 | 0.0001 |
Vacuole | 0.0038 | 0.0022 | 0.0033 | 0.0025 | 0.0087 | 0.0045 | 0.0039 | 0.002 | 0.006 | 0.0037 | 0.0052 | 0.0021 | 0.0024 | 0.0012 | 0.0042 | 0.0078 | 0.0031 | 0.0014 |
Vacuole Periphery | 0.0005 | 0 | 0.0002 | 0.0003 | 0.0014 | 0.0005 | 0.0008 | 0.0001 | 0.0005 | 0.0015 | 0.0009 | 0.0002 | 0.0001 | 0 | 0.0002 | 0.0002 | 0.0001 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 19.8454 | 15.1386 | 35.1047 | 66.1825 | 47.0009 | 14.8097 | 19.6116 | 20.1777 | 48.8711 | 53.5981 |
Translational Efficiency | 0.3471 | 0.5648 | 0.4836 | 0.6118 | 0.6707 | 0.5439 | 0.5444 | 0.5001 | 0.4686 | 0.5578 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
264 | 90 | 1110 | 928 | 1235 | 98 | 206 | 125 | 1499 | 188 | 1316 | 1053 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 615.41 | 766.16 | 1384.84 | 1025.93 | 772.36 | 774.65 | 1173.68 | 921.54 | 744.72 | 770.59 | 1351.79 | 1013.54 |
Standard Deviation | 91.68 | 149.13 | 354.01 | 188.58 | 157.62 | 142.34 | 233.33 | 160.82 | 159.76 | 145.69 | 346.57 | 188.55 |
Intensity Change Log 2 | — | 0.316098 | 1.170100 | 0.737313 | — | 0.004271 | 0.603694 | 0.254773 | — | 0.150920 | 0.882541 | 0.488833 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.002134 | 0.006363 | 0.019975 | 0.046193 | 0.006821 | 0.005406 | 0.037066 | 0.019101 | 0.005996 | 0.005864 | 0.022650 | 0.042977 |
Bud Neck | 0.053953 | 0.065382 | 0.021813 | 0.030752 | 0.057591 | 0.040079 | 0.018181 | 0.024644 | 0.056950 | 0.052192 | 0.021244 | 0.030027 |
Bud Site | 0.025726 | 0.039645 | 0.134559* | 0.164581* | 0.114356* | 0.042476 | 0.084620 | 0.105133* | 0.098747 | 0.041121 | 0.126742* | 0.157524* |
Cell Periphery | 0.019610 | 0.004227 | 0.512714* | 0.088176 | 0.117254* | 0.002951 | 0.118459* | 0.040557 | 0.100057* | 0.003562 | 0.450999* | 0.082523 |
Cytoplasm | 0.504750* | 0.231624* | 0.145418* | 0.355346* | 0.459919* | 0.272335* | 0.220699* | 0.346570* | 0.467815* | 0.252846* | 0.157203* | 0.354304* |
Cytoplasmic Foci | 0.140410 | 0.262106* | 0.004291 | 0.022836 | 0.075989 | 0.241756* | 0.051947 | 0.083126 | 0.087334 | 0.251498* | 0.011751 | 0.029993 |
Eisosomes | 0.010104 | 0.003902 | 0.005471 | 0.011476 | 0.012284 | 0.004932 | 0.007932 | 0.006486 | 0.011900 | 0.004439 | 0.005856 | 0.010884 |
Endoplasmic Reticulum | 0.003994 | 0.001930 | 0.033077 | 0.007867 | 0.004992 | 0.005802 | 0.017404 | 0.015469 | 0.004817 | 0.003948 | 0.030623 | 0.008769 |
Endosome | 0.007463 | 0.039092 | 0.001697 | 0.004767 | 0.003669 | 0.019592 | 0.005283 | 0.004550 | 0.004337 | 0.028927 | 0.002258 | 0.004741 |
Golgi | 0.003740 | 0.025375 | 0.001439 | 0.002703 | 0.002726 | 0.015020 | 0.001035 | 0.000918 | 0.002905 | 0.019977 | 0.001376 | 0.002491 |
Lipid Particles | 0.011150 | 0.007971 | 0.018383 | 0.006387 | 0.003405 | 0.010257 | 0.018376 | 0.008778 | 0.004769 | 0.009163 | 0.018381 | 0.006671 |
Mitochondria | 0.006908 | 0.007109 | 0.000786 | 0.000828 | 0.003071 | 0.011292 | 0.000063 | 0.000072 | 0.003747 | 0.009290 | 0.000673 | 0.000738 |
Mitotic Spindle | 0.000938 | 0.006139 | 0.000723 | 0.005032 | 0.000096 | 0.042370 | 0.000181 | 0.000073 | 0.000244 | 0.025025 | 0.000638 | 0.004443 |
None | 0.001143 | 0.000557 | 0.000313 | 0.000334 | 0.001342 | 0.002289 | 0.001967 | 0.000392 | 0.001307 | 0.001460 | 0.000572 | 0.000341 |
Nuclear Periphery | 0.001043 | 0.000380 | 0.000821 | 0.000151 | 0.000097 | 0.000709 | 0.000269 | 0.000303 | 0.000264 | 0.000552 | 0.000735 | 0.000169 |
Nuclear Periphery Foci | 0.001072 | 0.001151 | 0.002267 | 0.002499 | 0.000634 | 0.001594 | 0.005603 | 0.005152 | 0.000711 | 0.001382 | 0.002790 | 0.002814 |
Nucleolus | 0.000833 | 0.003681 | 0.000079 | 0.000063 | 0.000185 | 0.006620 | 0.000098 | 0.000113 | 0.000299 | 0.005213 | 0.000082 | 0.000069 |
Nucleus | 0.146407* | 0.082346 | 0.025927 | 0.030051 | 0.057888 | 0.113550 | 0.052233 | 0.088373 | 0.073477 | 0.098612 | 0.030045 | 0.036975 |
Peroxisomes | 0.014453 | 0.077800 | 0.000965 | 0.001294 | 0.002935 | 0.029902 | 0.001470 | 0.001096 | 0.004964 | 0.052832 | 0.001044 | 0.001270 |
Vacuole | 0.043305 | 0.131515 | 0.068647 | 0.217960* | 0.074322 | 0.128649 | 0.357023* | 0.248994* | 0.068860 | 0.130021 | 0.113788 | 0.221644* |
Vacuole Periphery | 0.000863 | 0.001705 | 0.000637 | 0.000702 | 0.000422 | 0.002419 | 0.000092 | 0.000102 | 0.000499 | 0.002077 | 0.000551 | 0.000631 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.45 | -11.51 | -14.37 | -9.36 | -6.83 | 0.61 | -3.18 | -2.30 | -2.27 | 1.44 | 0.24 | -9.25 | -13.17 | -10.57 | -5.75 |
Bud Neck | -0.64 | 4.27 | 3.51 | 2.95 | -2.64 | 1.68 | 12.20 | 5.75 | 1.17 | -1.34 | 0.84 | 11.91 | 9.34 | 2.64 | -3.12 |
Bud Site | -1.18 | -13.64 | -14.78 | -9.71 | -1.20 | 5.40 | 3.99 | 1.38 | -2.55 | -1.25 | 6.45 | -3.72 | -5.50 | -10.30 | -1.80 |
Cell Periphery | 3.15 | -42.52* | -7.98 | -13.49 | 37.26* | 16.22 | 2.08 | 7.71 | -3.82 | 2.98 | 16.41 | -29.41 | 4.04 | -14.20 | 33.62* |
Cytoplasm | 9.37 | 22.57 | 9.57 | -4.22 | -19.65 | 7.77 | 15.84 | 5.70 | -2.43 | -5.83 | 11.80 | 36.67 | 12.56 | -5.04 | -20.27 |
Cytoplasmic Foci | -4.73 | 15.26 | 12.91 | 9.99 | -10.96 | -6.25 | 5.42 | -0.03 | 5.67 | -2.64 | -9.03 | 21.48 | 14.45 | 12.62 | -8.50 |
Eisosomes | 2.25 | 1.84 | 0.07 | -3.90 | -4.69 | 2.25 | 4.53 | 3.83 | -0.33 | 0.14 | 3.75 | 6.11 | 1.98 | -2.67 | -4.52 |
Endoplasmic Reticulum | 1.51 | -11.79 | -2.43 | -5.90 | 11.35 | -0.35 | -4.16 | -1.86 | -1.51 | 0.17 | 0.50 | -12.44 | -2.83 | -2.65 | 10.88 |
Endosome | -4.53 | 4.75 | 2.07 | 5.02 | -2.82 | -5.28 | -2.94 | -1.24 | 5.00 | 1.23 | -6.60 | 6.34 | -0.01 | 6.46 | -2.53 |
Golgi | -2.20 | 3.15 | 1.46 | 2.35 | -1.28 | -1.72 | 8.74 | 7.85 | 2.03 | -0.18 | -2.85 | 7.27 | 1.25 | 2.98 | -1.33 |
Lipid Particles | 1.57 | -3.15 | 3.53 | 1.29 | 6.96 | -1.82 | -4.46 | -2.90 | 0.54 | 2.29 | -2.07 | -8.43 | -2.33 | 1.40 | 7.39 |
Mitochondria | 0.03 | 2.00 | 2.02 | 2.34 | 0.27 | -1.36 | 3.67 | 3.63 | 1.94 | -1.65 | -1.57 | 3.51 | 3.43 | 2.64 | 0.14 |
Mitotic Spindle | -1.11 | 0.09 | -2.55 | 0.20 | -2.73 | -2.91 | -2.06 | -1.60 | 2.90 | -0.07 | -3.05 | -2.88 | -3.17 | 2.67 | -2.75 |
None | 1.90 | 3.69 | 3.54 | 1.04 | -0.23 | -0.82 | -0.13 | 2.65 | 1.70 | 0.91 | -0.20 | 2.64 | 3.72 | 1.97 | 0.69 |
Nuclear Periphery | 0.89 | 0.29 | 1.17 | 1.61 | 14.10 | -3.27 | -6.67 | -3.58 | 0.40 | -1.11 | -1.81 | -3.88 | 0.10 | 2.70 | 12.03 |
Nuclear Periphery Foci | -0.34 | -1.54 | -1.73 | -2.05 | -0.79 | -1.46 | -8.03 | -3.97 | -0.67 | 0.28 | -1.69 | -10.07 | -8.33 | -0.35 | -0.03 |
Nucleolus | -1.23 | 2.69 | 2.77 | 1.49 | 1.21 | -1.56 | 3.10 | 1.94 | 1.57 | -1.00 | -2.06 | 3.99 | 4.33 | 2.15 | 0.97 |
Nucleus | 3.60 | 9.85 | 9.65 | 3.36 | -1.12 | -2.91 | -0.67 | -3.10 | 0.25 | -2.41 | -2.12 | 7.46 | 5.94 | 4.36 | -1.90 |
Peroxisomes | -3.51 | 3.26 | 3.20 | 4.40 | -1.01 | -2.92 | 5.21 | 7.48 | 3.15 | 0.57 | -4.85 | 5.15 | 4.98 | 5.27 | -0.87 |
Vacuole | -4.57 | -6.24 | -23.52 | -5.77 | -21.18 | -3.01 | -16.57 | -8.94 | -4.02 | 5.96 | -4.57 | -9.73 | -22.99 | -7.18 | -13.41 |
Vacuole Periphery | -1.29 | 1.08 | 0.82 | 1.70 | 0.08 | -2.19 | 6.13 | 5.94 | 2.58 | -0.70 | -2.84 | -0.72 | -0.26 | 2.57 | -0.03 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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