Standard name
Human Ortholog
Description GDP dissociation inhibitor; regulates vesicle traffic in secretory pathways by regulating the dissociation of GDP from the Sec4/Ypt/rab family of GTP binding proteins
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.05 0 0.1 0.07 0 0.06 – 0.06 0.09 0.05 0.08 0.1 0.08 0 0.09 0.1 0 0.08 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.96 0.94 0.94 0.93 – 0.87 0.87 0.9 0.9 0.83 0.99 1.0 0.98 0.96 0.99 0.91 0.98 0.93 0.91 0.95 0.92 0.89
Endoplasmic Reticulum 0 0 0 0 0.06 0.05 – 0.12 0.1 0.07 0.07 0.11 0 0 0 0.07 0 0 0 0 0.05 0 0.06 0.05
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 2 0 0 1 – 3 3 5 2 10 0 0 0 0 1 0 0 0 2 0 2 2
Bud 1 0 3 1 1 0 – 4 4 3 3 3 2 2 2 0 1 1 0 0 2 0 3 3
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 13 1 34 16 9 20 – 29 29 20 29 47 22 8 30 14 9 10 0 0 1 0 1 1
Cytoplasm 268 128 330 219 321 321 – 442 280 398 340 389 279 200 330 132 213 117 269 119 325 191 458 419
Endoplasmic Reticulum 4 0 12 8 19 17 – 60 32 30 25 51 5 1 3 9 7 4 2 0 18 6 27 22
Endosome 1 0 1 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 1 1 2 0 0 6
Golgi 0 0 1 1 1 0 – 1 0 2 0 2 0 0 0 1 0 4 0 0 0 0 0 0
Mitochondria 0 2 0 0 0 0 – 4 1 5 3 9 0 0 0 2 2 1 0 1 0 0 0 2
Nucleus 0 0 0 1 1 1 – 0 2 2 1 1 0 0 1 0 1 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 2
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1
Vac/Vac Membrane 0 0 0 0 2 0 – 0 1 2 0 0 2 0 0 0 0 0 0 1 0 1 2 1
Unique Cell Count 273 130 342 232 340 344 507 322 443 379 471 283 200 336 138 215 128 276 128 358 203 499 471
Labelled Cell Count 287 131 383 246 354 360 543 352 468 404 512 310 211 366 158 234 140 276 128 358 203 499 471


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 29.7 23.8 29.4 29.3 28.6 26.6 – 25.5 24.9 22.1 21.7 24.3 26.0 24.9 30.0 19.6 22.8 27.3 28.6 31.4 32.8
Std Deviation (1e-4) 4.5 6.7 5.1 4.9 5.4 4.4 – 5.3 5.3 4.7 4.5 5.5 4.5 5.3 5.5 4.6 6.2 7.9 4.7 5.6 7.1
Intensity Change (Log2) – – – -0.0 -0.04 -0.14 – -0.21 -0.24 -0.41 -0.44 -0.27 -0.18 -0.24 0.03 -0.58 -0.37 -0.1 -0.04 0.1 0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 1.8 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.3 -3.9 -2.0 – -2.3 -0.4 -3.0 -1.1 0 -0.9 -2.5 -0.5 0.1 -2.5 -0.7
Cytoplasm -1.2 -1.3 -1.9 – -4.6 -4.5 -3.6 -3.5 -6.1 1.7 2.7 1.4 -0.4 1.9 -2.3
Endoplasmic Reticulum 0 1.3 0.9 – 4.3 3.3 2.0 1.9 3.9 -1.3 0 -2.3 1.5 -0.2 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 20.6679 25.0177 22.6115 21.5074 21.0972 23.3553 24.6279 27.4034 26.6326 24.742 23.2354 26.3871 22.3473 27.4379 26.078 26.8196 26.5633 26.022
Actin 0.0104 0.0012 0.0194 0.0451 0.0009 0.0009 0.0033 0.0019 0.0062 0.0124 0.0056 0.0004 0.0113 0.0015 0.0184 0.0006 0.002 0.0026
Bud 0.0025 0.0001 0.0071 0.0003 0.0008 0.0002 0.0002 0.0003 0.0005 0.0001 0.0002 0 0.0005 0.0003 0.0009 0.0001 0.0002 0.0001
Bud Neck 0.0008 0.0007 0.0015 0.0012 0.0005 0.013 0.0005 0.0004 0.0005 0.0007 0.0014 0.0019 0.0019 0.0003 0.0008 0.0005 0.0011 0.0039
Bud Periphery 0.0051 0 0.0041 0.0003 0.0006 0.0001 0.0003 0.0002 0.0002 0.0001 0.0002 0 0.0008 0.0001 0.0007 0 0 0.0002
Bud Site 0.0014 0.0002 0.0025 0.0011 0.0008 0.0002 0.0009 0.0049 0.0005 0.0003 0.001 0 0.0067 0.0006 0.002 0.0001 0.0008 0.0002
Cell Periphery 0.0008 0.0002 0.0004 0.0003 0.0002 0.0001 0.0006 0.0006 0.0002 0.0001 0.0001 0.0001 0.0015 0.0003 0.0003 0.0001 0.0002 0.0001
Cytoplasm 0.8915 0.9872 0.9127 0.9172 0.9372 0.9498 0.927 0.9686 0.9726 0.959 0.9149 0.9783 0.8567 0.9599 0.9481 0.9897 0.9882 0.9494
Cytoplasmic Foci 0.0198 0.0005 0.0114 0.0016 0.0036 0.0051 0.0132 0.0046 0.0007 0.0053 0.0044 0.001 0.0116 0.0008 0.0018 0.0015 0.0018 0.0023
Eisosomes 0.0001 0 0.0002 0.0002 0 0 0 0.0001 0.0005 0.0002 0.0001 0 0.0007 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0142 0.0064 0.0088 0.0239 0.0247 0.0115 0.0228 0.0102 0.0056 0.003 0.0193 0.0139 0.0336 0.0178 0.0053 0.0039 0.0016 0.0081
Endosome 0.0087 0.001 0.0094 0.0012 0.0151 0.0062 0.0057 0.0012 0.0013 0.0041 0.0103 0.0007 0.0141 0.0007 0.0045 0.0008 0.0005 0.0045
Golgi 0.0064 0.0001 0.0027 0.0016 0.0012 0.0006 0.0015 0.0008 0.0004 0.0016 0.0013 0.0001 0.0043 0.0001 0.0018 0.0001 0.0001 0.0049
Lipid Particles 0.0104 0 0.0034 0.0008 0.0003 0.0006 0.0047 0.0003 0.0006 0.0047 0.0012 0.0001 0.0111 0.0001 0.0006 0 0.0002 0.0146
Mitochondria 0.0031 0 0.0015 0.0007 0.0011 0.0004 0.0029 0.0003 0.005 0.0015 0.0007 0.0001 0.004 0.0002 0.0017 0 0 0.0025
None 0.0051 0.0001 0.002 0.0004 0.0009 0.0007 0.0027 0.0017 0.0004 0.001 0.0021 0.0009 0.0086 0.0004 0.0007 0.0002 0.0002 0.0001
Nuclear Periphery 0.0022 0.0004 0.0047 0.001 0.0027 0.0012 0.0026 0.0007 0.0024 0.0007 0.0112 0.0005 0.0022 0.0039 0.0069 0.0007 0.0007 0.0017
Nucleolus 0.0002 0 0.0003 0 0.0001 0.0003 0.0002 0.0002 0.0001 0 0.0014 0.0001 0.0023 0.0005 0.0001 0 0 0.0002
Nucleus 0.0022 0.0005 0.0019 0.0009 0.0025 0.0016 0.0019 0.0011 0.0006 0.0006 0.0106 0.0008 0.0022 0.0064 0.0026 0.0006 0.0013 0.0006
Peroxisomes 0.0022 0 0.001 0.0005 0.0001 0.0003 0.003 0.0002 0.0004 0.0025 0.0003 0 0.0183 0 0.0004 0 0.0001 0.001
Punctate Nuclear 0.0095 0 0.0017 0.0003 0.0004 0.0059 0.0024 0.0003 0.0004 0.0009 0.0117 0.0001 0.0016 0.0052 0.0004 0.0001 0.0004 0.0024
Vacuole 0.0027 0.0013 0.0026 0.0012 0.0055 0.001 0.0033 0.001 0.0006 0.0012 0.0013 0.0008 0.0053 0.0007 0.0016 0.0011 0.0004 0.0004
Vacuole Periphery 0.0006 0.0001 0.0006 0.0001 0.001 0.0002 0.0003 0.0001 0.0002 0.0001 0.0004 0.0001 0.0006 0.0001 0.0005 0.0001 0.0001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 166.4058 91.6544 83.1252 120.7704 97.0622 102.472 123.0106 128.5691 114.4014 121.8674
Translational Efficiency 1.2341 1.7286 1.5381 1.1875 1.2831 2.0544 1.4262 1.0611 1.2721 1.1606

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
94 863 189 1201 122 1114 1089 275 216 1977 1278 1476

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2255.84 2042.69 2826.09 2699.32 1864.48 1921.29 2671.20 2837.13 2034.79 1974.28 2694.11 2725.00
Standard Deviation 378.26 396.56 457.19 555.85 338.05 347.36 416.13 591.30 405.54 374.52 426.02 565.18
Intensity Change Log 2 — -0.143194 0.325143 0.258931 — 0.043302 0.518715 0.605659 — -0.055807 0.415964 0.426205

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.003602 0.000579 0.001760 0.001346 0.001049 0.000960 0.001129 0.001048 0.002160 0.000793 0.001222 0.001291
Bud Neck 0.011230 0.001660 0.016968 0.004814 0.001441 0.001383 0.004108 0.005385 0.005701 0.001504 0.006010 0.004920
Bud Site 0.058431 0.018335 0.026945 0.032340 0.025836 0.024771 0.019591 0.044205 0.040021 0.021962 0.020679 0.034551
Cell Periphery 0.008834 0.005306 0.043549 0.016353 0.009508 0.002430 0.019698 0.017390 0.009214 0.003685 0.023225 0.016547
Cytoplasm 0.381429* 0.859820* 0.429157* 0.682713* 0.822081* 0.864839* 0.730237* 0.672829* 0.630316* 0.862648* 0.685711* 0.680871*
Cytoplasmic Foci 0.030765 0.019078 0.029591 0.010189 0.032727 0.028564 0.008205 0.009747 0.031873 0.024423 0.011367 0.010107
Eisosomes 0.000040 0.000006 0.000028 0.000022 0.000011 0.000009 0.000014 0.000017 0.000023 0.000008 0.000016 0.000021
Endoplasmic Reticulum 0.001311 0.003759 0.003438 0.000318 0.003800 0.003860 0.000448 0.000091 0.002717 0.003815 0.000890 0.000276
Endosome 0.016330 0.002409 0.008576 0.003285 0.002647 0.003705 0.000883 0.001603 0.008602 0.003139 0.002021 0.002972
Golgi 0.041391 0.012530 0.029891 0.005682 0.021196 0.008269 0.004564 0.003172 0.029985 0.010129 0.008310 0.005214
Lipid Particles 0.088557 0.028083 0.129938* 0.059789 0.037894 0.017221 0.080138 0.057415 0.059942 0.021963 0.087503 0.059347
Mitochondria 0.000557 0.000028 0.000037 0.000404 0.000034 0.000157 0.000029 0.000379 0.000262 0.000101 0.000030 0.000399
Mitotic Spindle 0.016031 0.001025 0.002824 0.001768 0.002930 0.001498 0.000491 0.003647 0.008632 0.001292 0.000836 0.002118
None 0.000135 0.001241 0.000631 0.000690 0.000773 0.001225 0.001427 0.000441 0.000495 0.001232 0.001309 0.000644
Nuclear Periphery 0.000122 0.000058 0.000986 0.000139 0.000052 0.000050 0.000191 0.000119 0.000083 0.000053 0.000308 0.000135
Nuclear Periphery Foci 0.014599 0.011913 0.035690 0.007095 0.007756 0.005238 0.013360 0.008815 0.010734 0.008152 0.016663 0.007415
Nucleolus 0.000095 0.000042 0.000060 0.000134 0.000024 0.000064 0.000065 0.000117 0.000055 0.000054 0.000064 0.000131
Nucleus 0.004193 0.005506 0.015931 0.009892 0.002591 0.005097 0.009741 0.006138 0.003288 0.005275 0.010657 0.009193
Peroxisomes 0.001325 0.000255 0.000141 0.000570 0.000208 0.000328 0.000179 0.000481 0.000694 0.000296 0.000173 0.000553
Vacuole 0.312727* 0.026112 0.218459* 0.159563 0.024180 0.027994 0.104740 0.162328 0.149752 0.027173 0.121557 0.160078
Vacuole Periphery 0.008296 0.002256 0.005402 0.002895 0.003262 0.002338 0.000762 0.004634 0.005453 0.002302 0.001449 0.003219

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.90 1.24 1.87 -7.02 1.84 0.15 -0.64 -0.11 -0.70 1.37 2.38 0.94 1.15 -4.99 0.59
Bud Neck 8.96 -3.26 5.40 -11.41 8.32 0.01 -8.39 -7.16 -7.55 -2.29 7.10 -0.87 0.43 -14.22 2.02
Bud Site 3.44 2.14 1.73 -5.21 -1.06 0.20 0.83 -2.70 -3.31 -3.86 2.95 2.69 0.11 -5.26 -4.68
Cell Periphery 1.33 -7.16 -4.23 -6.37 5.95 3.46 -3.75 -1.31 -6.90 2.97 3.69 -7.56 -2.82 -10.07 6.15
Cytoplasm -15.99 -1.22 -8.74 20.55 -9.39 -2.05 5.30 7.60 13.13 4.52 -10.18 -1.84 -0.52 25.99 2.91
Cytoplasmic Foci 2.05 -1.42 3.65 3.33 5.21 0.65 4.10 2.55 3.68 -2.61 1.55 4.59 4.53 6.60 -0.02
Eisosomes 7.62 1.63 3.22 -9.40 2.07 0.64 -2.25 -2.64 -4.67 -1.03 6.09 1.72 0.16 -9.07 -2.27
Endoplasmic Reticulum -2.73 -2.06 1.75 3.42 2.74 -0.02 1.63 1.81 4.88 5.15 -0.98 1.43 1.95 5.75 3.61
Endosome 2.65 0.62 2.58 -0.19 4.65 -1.56 3.46 2.01 3.68 -1.35 2.07 2.88 2.58 1.50 -0.53
Golgi 1.10 -0.30 1.93 6.00 7.76 2.44 3.18 3.54 5.13 2.23 2.26 2.68 3.45 6.40 4.33
Lipid Particles 3.75 -3.26 1.85 -6.08 7.49 2.51 -4.87 -1.23 -5.25 4.36 4.43 -4.09 0.34 -9.55 7.93
Mitochondria 1.55 1.49 0.27 -2.08 -1.99 -2.25 0.33 -1.05 -0.46 -1.07 0.67 1.54 -0.78 -1.55 -2.31
Mitotic Spindle 1.59 1.20 1.36 -1.43 1.05 0.94 1.64 -0.13 -1.16 -1.89 1.82 1.99 1.34 -1.46 -2.09
None -6.30 -4.74 -5.25 3.15 0.26 -1.34 -1.77 1.21 3.08 4.27 -3.45 -3.34 -0.59 3.71 3.55
Nuclear Periphery 5.28 -7.16 -2.03 -7.21 6.82 0.14 -4.60 -4.69 -4.43 1.93 2.64 -6.96 -5.09 -7.46 4.49
Nuclear Periphery Foci 1.19 -5.95 4.02 3.94 9.33 2.09 -4.78 -0.63 -2.50 3.48 2.15 -4.90 2.95 0.93 9.94
Nucleolus 3.12 1.24 -2.09 -5.02 -3.50 -6.03 -8.89 -4.56 -2.63 -2.70 -0.35 -1.70 -4.91 -5.03 -4.47
Nucleus -0.16 -6.08 -7.13 -8.64 1.07 -4.72 -11.06 -5.63 -3.73 1.70 -2.55 -10.55 -10.86 -10.30 -1.70
Peroxisomes 3.45 3.96 2.38 -2.10 -3.33 -3.01 0.77 -2.15 -1.39 -2.35 2.58 3.81 0.65 -2.23 -3.80
Vacuole 11.93 4.54 5.83 -27.70 1.38 -0.77 -12.47 -12.92 -13.67 -6.94 8.41 2.66 -1.38 -31.17 -10.67
Vacuole Periphery 1.01 0.02 1.15 0.26 2.89 0.66 1.96 0.12 -0.39 -1.38 1.20 2.00 1.29 0.16 -1.60
Standard name
Human Ortholog
Description GDP dissociation inhibitor; regulates vesicle traffic in secretory pathways by regulating the dissociation of GDP from the Sec4/Ypt/rab family of GTP binding proteins
Localization
Cell Percentages cytoplasm (96%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Gdi1

Gdi1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gdi1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available