Standard name
Human Ortholog
Description Protein of unknown function; required for efficient 5' processing of mitochondrial tRNAs, for respiratory growth and mitochondrial genome maintenance; null mutant has decreased levels of cardiolipin and phosphatidylethanolamine; localizes to the matrix side of the inner mitochondrial membrane
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 – 0 – 0 – 0 0 0 0 0 0 0 – – 0 0 0 0.05 0 0 0
Bud 0 0 0 – 0 – 0 – 0 0.08 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 – 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 – 0 – 0 0 0 0 0 0 0 – – 0 – – – – – –
Cell Periphery 0 0 0 – 0 – 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Cytoplasm 0.63 0.83 0.77 – 0.87 – 0.53 – 0.27 0.08 0.13 0.17 0.95 0.95 0.95 – – 0.53 0.21 0.43 0.11 0.3 0.32 0.33
Endoplasmic Reticulum 0.08 0 0 – 0.05 – 0 – 0.06 0.08 0 0 0 0 0 – – 0.07 0 0 0 0 0 0
Endosome 0 0 0 – 0 – 0 – 0 0 0 0 0 0 0 – – 0.17 0 0 0.1 0 0 0
Golgi 0 0 0 – 0 – 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Mitochondria 0.37 0.2 0 – 0 – 0.39 – 0.8 0.88 0.86 0.8 0 0 0 – – 0.13 0.58 0.35 0.61 0.48 0.43 0.39
Nucleus 0 0 0 – 0 – 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 – 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 – 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 – 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0.05 – 0.09 – 0 0.08 0.07 0.08 0 0 0 – – 0.19 0 0 0 0 0 0.05
Vac/Vac Membrane 0 0 0.15 – 0 – 0 – 0 0 0 0 0 0 0 – – 0.05 0 0.07 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 – 0 – 0 – 0 0 1 1 3 0 0 – – 2 2 2 4 4 9 15
Bud 1 0 0 – 0 – 1 – 1 2 2 4 1 0 2 – – 2 0 1 0 1 7 9
Bud Neck 0 0 1 – 0 – 2 – 1 0 1 2 0 2 4 – – 0 1 1 0 2 5 10
Bud Site 0 0 0 – 0 – 0 – 1 0 0 0 0 0 0 – – 1 – – – – – –
Cell Periphery 1 0 1 – 0 – 0 – 0 1 1 3 2 1 0 – – 2 0 1 0 1 2 2
Cytoplasm 55 118 20 – 48 – 45 – 29 2 16 24 165 415 454 – – 57 26 71 9 50 97 126
Endoplasmic Reticulum 7 1 0 – 3 – 0 – 7 2 5 4 1 3 0 – – 8 1 1 2 1 2 5
Endosome 0 0 0 – 2 – 2 – 0 0 0 0 2 11 7 – – 18 5 3 8 3 12 10
Golgi 0 0 0 – 0 – 0 – 0 0 0 0 0 1 1 – – 0 0 0 0 0 3 0
Mitochondria 32 28 1 – 0 – 33 – 86 21 104 115 0 1 0 – – 14 73 58 52 81 134 148
Nucleus 0 2 0 – 2 – 2 – 3 1 3 6 1 0 0 – – 2 0 2 0 0 2 1
Nuclear Periphery 0 0 0 – 0 – 0 – 0 0 2 0 0 0 0 – – 0 0 0 0 1 0 0
Nucleolus 0 0 0 – 0 – 0 – 0 0 1 0 0 0 0 – – 1 0 0 0 0 0 2
Peroxisomes 0 0 0 – 1 – 0 – 0 0 0 2 0 1 11 – – 1 0 0 1 0 0 1
SpindlePole 0 3 0 – 3 – 8 – 2 2 8 12 2 10 9 – – 21 4 6 1 5 9 17
Vac/Vac Membrane 1 2 4 – 1 – 3 – 0 0 2 4 2 5 10 – – 5 5 11 1 6 12 18
Unique Cell Count 87 143 26 55 85 108 24 121 143 174 438 480 108 126 166 85 171 309 384
Labelled Cell Count 98 154 27 60 96 130 31 146 177 179 450 498 134 126 166 85 171 309 384


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 3.5 5.0 – 5.6 – 4.3 – 3.3 3.1 3.4 3.6 5.0 5.1 4.9 4.0 4.7 4.9 5.5 5.4 5.3
Std Deviation (1e-4) 0.8 0.7 1.3 – 1.9 – 2.0 – 0.7 0.4 1.1 0.5 1.1 1.5 1.6 1.2 1.3 1.1 1.8 1.8 1.7
Intensity Change (Log2) – – – – 0.16 – -0.24 – -0.6 -0.69 -0.57 -0.51 -0.01 0.01 -0.05 -0.35 -0.1 -0.03 0.12 0.11 0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.6752 4.2374 3.9945 3.136 3.8158 3.9466 0.9377 1.0538 1.2486 0.8823 0.9215 0.8983 -1.2298 -0.8244 -1.0658 -0.9366 -0.664 -1.0928
Actin 0.0586 0.0338 0.03 0.0439 0.0351 0.0226 0.0577 0.0043 0.021 0.0028 0.0352 0.0133 0.0534 0.0292 0.0507 0.0144 0.0365 0.0127
Bud 0.0011 0.0073 0.0045 0.0035 0.0057 0.001 0.0027 0.0024 0.0039 0.0051 0.008 0.0012 0.0015 0.0064 0.0032 0.0022 0.0017 0.0011
Bud Neck 0.0025 0.0051 0.0072 0.0106 0.0019 0.0284 0.0097 0.0053 0.0087 0.0003 0.0014 0.0163 0.0025 0.0027 0.005 0.0351 0.0008 0.0135
Bud Periphery 0.0022 0.0146 0.0133 0.0085 0.0204 0.0031 0.0099 0.0077 0.017 0.0303 0.0255 0.004 0.0035 0.017 0.0055 0.0045 0.0021 0.0039
Bud Site 0.0072 0.0937 0.0316 0.0267 0.0168 0.0034 0.042 0.0455 0.0294 0.0008 0.0061 0.0023 0.0119 0.0529 0.0097 0.0062 0.0021 0.0026
Cell Periphery 0.0026 0.0131 0.0106 0.0024 0.0025 0.0028 0.0056 0.0048 0.0195 0.0017 0.0035 0.0025 0.0054 0.0039 0.0016 0.0012 0.0011 0.0056
Cytoplasm 0.0454 0.1076 0.0864 0.0269 0.1446 0.1049 0.0448 0.0813 0.0676 0.0494 0.0837 0.0638 0.0605 0.1012 0.0726 0.0978 0.1648 0.1039
Cytoplasmic Foci 0.1087 0.1078 0.1275 0.1317 0.0861 0.1403 0.0858 0.1212 0.0768 0.0491 0.0935 0.1508 0.1086 0.159 0.129 0.0857 0.1177 0.1029
Eisosomes 0.0038 0.0037 0.0026 0.0013 0.0007 0.0027 0.0036 0.0017 0.0052 0.0008 0.0019 0.0017 0.0014 0.0013 0.0007 0.0004 0.0003 0.001
Endoplasmic Reticulum 0.0073 0.0039 0.0127 0.0054 0.0031 0.0055 0.0272 0.0134 0.0054 0.0049 0.0046 0.0036 0.013 0.0055 0.006 0.0624 0.0125 0.0062
Endosome 0.129 0.0683 0.0805 0.1101 0.0642 0.0911 0.0813 0.0539 0.0813 0.1439 0.1097 0.0796 0.2002 0.1492 0.1688 0.0847 0.1066 0.1249
Golgi 0.053 0.0302 0.0532 0.0586 0.0461 0.054 0.0205 0.0159 0.0201 0.0211 0.0286 0.0363 0.0451 0.0411 0.0522 0.0298 0.0141 0.0573
Lipid Particles 0.0567 0.0252 0.0233 0.0282 0.0581 0.039 0.0489 0.02 0.0221 0.0291 0.0456 0.0332 0.0746 0.0202 0.0153 0.0185 0.0263 0.0478
Mitochondria 0.143 0.1434 0.1496 0.3494 0.2172 0.1709 0.2258 0.2818 0.2225 0.3848 0.2431 0.2489 0.223 0.179 0.2553 0.2736 0.2494 0.3633
None 0.2593 0.2614 0.2602 0.0987 0.1674 0.2503 0.2449 0.2672 0.3189 0.2433 0.2357 0.2537 0.1025 0.1652 0.16 0.2002 0.2209 0.1009
Nuclear Periphery 0.0127 0.0009 0.0106 0.0014 0.002 0.0006 0.0054 0.012 0.0028 0.0013 0.0034 0.0006 0.0079 0.0022 0.0022 0.0026 0.0131 0.0022
Nucleolus 0.0041 0.0023 0.0022 0.001 0.0013 0.0013 0.0077 0.0025 0.002 0.0004 0.0019 0.0008 0.0025 0.001 0.0008 0.0007 0.0005 0.001
Nucleus 0.0068 0.002 0.0113 0.0011 0.0076 0.0014 0.0051 0.0032 0.002 0.0006 0.0024 0.0009 0.0041 0.0016 0.0014 0.0019 0.0021 0.0016
Peroxisomes 0.0601 0.0635 0.0572 0.0718 0.1021 0.0658 0.0439 0.0341 0.0398 0.0117 0.0324 0.0753 0.0279 0.0213 0.0375 0.0576 0.0054 0.0302
Punctate Nuclear 0.0151 0.0017 0.0026 0.0008 0.0075 0.0019 0.0059 0.0053 0.0086 0.0004 0.017 0.0015 0.0091 0.0009 0.0013 0.0009 0.0084 0.002
Vacuole 0.0155 0.0086 0.0179 0.0127 0.0075 0.0065 0.0172 0.0118 0.0202 0.0116 0.0107 0.0069 0.034 0.0299 0.0159 0.0154 0.0095 0.0097
Vacuole Periphery 0.005 0.0017 0.0047 0.0055 0.0022 0.0023 0.0044 0.0047 0.0051 0.0068 0.0062 0.003 0.0074 0.0092 0.0055 0.0041 0.0039 0.0059

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.6337 2.2367 3.3194 6.1255 4.866 6.2517 8.198 12.2109 10.3577 9.9766
Translational Efficiency 0.4464 2.4409 1.558 0.741 1.1373 0.7416 0.71 0.3577 0.4785 0.582

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2258 1105 2098 1366 1683 2276 1997 1020 3941 3381 4095 2386

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 663.66 650.94 774.02 735.96 587.54 739.63 706.44 731.62 631.15 710.64 741.06 734.10
Standard Deviation 76.08 86.19 85.92 133.80 82.84 89.25 115.70 125.75 87.55 97.57 107.01 130.44
Intensity Change Log 2 — -0.027920 0.221927 0.149183 — 0.332117 0.265880 0.316407 — 0.152364 0.242733 0.230127

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000937 0.000823 0.001954 0.004607 0.000509 0.000521 0.002938 0.004398 0.000754 0.000619 0.002434 0.004517
Bud Neck 0.037020 0.045819 0.042001 0.026324 0.043784 0.072266 0.063296 0.034811 0.039909 0.063623 0.052386 0.029952
Bud Site 0.011105 0.034239 0.033105 0.062388 0.013774 0.022655 0.069506 0.048590 0.012245 0.026441 0.050856 0.056490
Cell Periphery 0.000202 0.000201 0.000162 0.000254 0.000246 0.000166 0.000257 0.000220 0.000221 0.000177 0.000208 0.000240
Cytoplasm 0.139009* 0.133600* 0.305926* 0.308076* 0.165561* 0.181239* 0.198992* 0.269544* 0.150348* 0.165669* 0.253778* 0.291604*
Cytoplasmic Foci 0.376591* 0.291617* 0.110222 0.122878 0.367794* 0.316804* 0.141280 0.149701 0.372835* 0.308572* 0.125368 0.134345
Eisosomes 0.000483 0.000176 0.000093 0.000157 0.000400 0.000169 0.000142 0.000164 0.000448 0.000171 0.000117 0.000160
Endoplasmic Reticulum 0.002724 0.000956 0.006201 0.003941 0.001395 0.000664 0.003523 0.003261 0.002156 0.000760 0.004895 0.003650
Endosome 0.042816 0.078887 0.016460 0.023696 0.055452 0.054160 0.043799 0.029790 0.048212 0.062241 0.029792 0.026301
Golgi 0.046939 0.048381 0.003539 0.007852 0.034210 0.021335 0.014250 0.008532 0.041503 0.030174 0.008762 0.008143
Lipid Particles 0.033081 0.009959 0.006482 0.005859 0.031564 0.014553 0.007371 0.007588 0.032433 0.013051 0.006916 0.006598
Mitochondria 0.149780* 0.157192* 0.018469 0.013777 0.117388* 0.112426* 0.054220 0.016778 0.135947* 0.127057* 0.035904 0.015060
Mitotic Spindle 0.002961 0.020934 0.052713 0.038203 0.002767 0.011068 0.058082 0.033346 0.002879 0.014293 0.055331 0.036126
None 0.000979 0.003300 0.002231 0.003469 0.010798 0.002495 0.006841 0.005806 0.005172 0.002758 0.004479 0.004468
Nuclear Periphery 0.000748 0.000292 0.004467 0.001336 0.000640 0.000513 0.001391 0.001359 0.000702 0.000441 0.002967 0.001346
Nuclear Periphery Foci 0.000312 0.000426 0.001749 0.001788 0.000582 0.000188 0.001214 0.001654 0.000427 0.000266 0.001488 0.001731
Nucleolus 0.001131 0.003825 0.004142 0.002511 0.005257 0.002940 0.005882 0.004759 0.002893 0.003229 0.004990 0.003472
Nucleus 0.047233 0.032270 0.262374* 0.146395* 0.054662 0.059505 0.116833 0.157407* 0.050405 0.050604 0.191398* 0.151102*
Peroxisomes 0.050799 0.071762 0.025681 0.043840 0.047425 0.054672 0.071171 0.072908 0.049358 0.060258 0.047865 0.056267
Vacuole 0.048640 0.056100 0.099278 0.179263 0.039677 0.065448 0.133295 0.146166 0.044813 0.062393 0.115867 0.165114
Vacuole Periphery 0.006509 0.009241 0.002752 0.003383 0.006113 0.006214 0.005718 0.003220 0.006340 0.007204 0.004198 0.003313

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.67 -4.63 -5.62 -6.10 -3.49 -0.11 -8.77 -3.65 -3.65 -0.93 1.31 -9.24 -6.33 -6.75 -2.73
Bud Neck -2.76 -1.88 6.01 7.70 8.01 -7.84 -4.96 3.90 11.45 8.74 -9.37 -5.28 6.99 15.16 12.17
Bud Site -10.06 -9.92 -10.57 -3.57 -4.45 -5.46 -16.20 -8.20 -5.47 5.11 -11.29 -18.95 -13.36 -7.24 0.34
Cell Periphery 0.09 2.53 -0.83 -0.77 -4.18 3.04 0.66 2.43 -1.28 1.88 2.36 1.90 0.91 -1.86 -1.23
Cytoplasm 0.55 -23.18 -18.91 -16.75 0.11 -2.34 -5.05 -10.49 -9.01 -6.55 -3.33 -20.11 -21.15 -17.62 -4.78
Cytoplasmic Foci 10.07 46.62 44.16 24.27 0.30 6.87 36.24 32.44 27.18 0.21 11.80 58.67 54.47 38.71 0.75
Eisosomes 7.86 10.16 8.75 2.76 -6.29 5.22 5.91 5.49 1.64 -2.29 9.50 11.46 10.22 3.28 -5.93
Endoplasmic Reticulum 4.59 -8.21 -3.59 -10.70 5.79 4.50 -8.92 -6.65 -9.48 0.24 6.44 -10.68 -6.53 -15.57 4.43
Endosome -9.05 14.93 12.00 15.78 -1.95 0.41 5.45 10.34 11.17 5.98 -6.02 12.38 15.68 19.70 4.31
Golgi -0.12 22.01 19.38 14.87 -2.24 6.44 11.33 14.36 11.08 4.40 6.66 23.76 24.04 18.51 2.12
Lipid Particles 13.37 17.25 18.31 5.21 2.59 9.14 14.26 14.51 8.06 0.63 15.09 22.33 23.35 10.32 2.33
Mitochondria -0.47 27.77 29.04* 20.66 4.51 0.85 13.14 21.08 23.20 10.96 2.15 28.76 35.77* 31.49 11.84
Mitotic Spindle -7.74 -15.60 -9.52 -3.43 3.71 -6.79 -16.26 -7.50 -5.00 5.50 -10.03 -22.49 -12.12 -6.92 6.46
None -3.46 -6.22 -3.79 1.12 -1.29 4.73 2.45 3.09 -2.14 0.83 2.84 1.36 1.85 -1.16 0.59
Nuclear Periphery 2.03 -7.50 -4.10 -9.51 5.59 1.23 -7.28 -6.16 -7.31 0.76 1.89 -9.09 -6.33 -11.29 5.40
Nuclear Periphery Foci -1.26 -8.82 -6.85 -6.33 -0.55 4.69 -4.45 -4.70 -6.34 -1.77 2.12 -9.69 -8.30 -9.35 -1.69
Nucleolus -4.30 -5.96 -2.76 2.98 4.47 2.70 -1.09 0.91 -2.22 2.25 -0.71 -4.38 -0.25 0.55 4.65
Nucleus 4.15 -36.00 -19.26 -20.87 13.79 -1.32 -14.47 -16.61 -16.09 -5.60 -0.20 -36.09 -25.41 -24.78 6.13
Peroxisomes -4.73 9.90 5.24 8.29 -2.48 -2.28 -4.43 -1.93 -0.49 1.22 -4.32 2.32 2.05 5.34 0.27
Vacuole -2.31 -16.96 -27.99 -25.38 -17.81 -9.18 -25.89 -21.98 -17.93 -4.48 -7.95 -30.12 -35.43 -30.93 -15.85
Vacuole Periphery -2.75 10.58 8.71 7.06 0.20 -0.14 2.29 7.25 6.77 4.83 -1.62 8.31 11.33 9.71 3.65
Standard name
Human Ortholog
Description Protein of unknown function; required for efficient 5' processing of mitochondrial tRNAs, for respiratory growth and mitochondrial genome maintenance; null mutant has decreased levels of cardiolipin and phosphatidylethanolamine; localizes to the matrix side of the inner mitochondrial membrane
Localization
Cell Percentages mitochondrion (60%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Gep5

Gep5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gep5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available