| Standard name | |
|---|---|
| Human Ortholog | |
| Description (SGD) | Type 1 S/T protein phosphatase (PP1) catalytic subunit; involved in glycogen metabolism, sporulation and mitotic progression; interacts with multiple regulatory subunits; regulates actomyosin ring formation; subunit of CPF; recruited to mating projections by Afr1p interaction; regulates nucleocytoplasmic shuttling of Hxk2p; import into the nucleus is inhibited during spindle assembly checkpoint arrest; involved in dephosphorylating Rps6a/b and Bnr1p |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | – | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | 12 | 2 | 4 | 1 | 6 | 0 | 0 | 0 | 1 |
| Bud | – | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | 3 | 1 | 0 | 0 | 10 | 0 | 0 | 0 | 3 |
| Bud Neck | – | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 |
| Bud Site | – | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | – | – | – | – | – | – |
| Cell Periphery | – | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | 5 | 6 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
| Cytoplasm | – | 12 | – | – | – | – | – | – | – | – | – | – | – | – | – | 148 | 90 | 98 | 5 | 10 | 1 | 1 | 2 | 3 |
| Endoplasmic Reticulum | – | 4 | – | – | – | – | – | – | – | – | – | – | – | – | – | 21 | 7 | 11 | 8 | 5 | 0 | 0 | 1 | 4 |
| Endosome | – | 3 | – | – | – | – | – | – | – | – | – | – | – | – | – | 15 | 4 | 2 | 0 | 9 | 0 | 0 | 0 | 0 |
| Golgi | – | 4 | – | – | – | – | – | – | – | – | – | – | – | – | – | 162 | 148 | 81 | 0 | 1 | 0 | 0 | 0 | 2 |
| Mitochondria | – | 1 | – | – | – | – | – | – | – | – | – | – | – | – | – | 14 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| Nucleus | – | 4 | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 1 | 0 | 1 | 16 | 0 | 0 | 0 | 0 |
| Nuclear Periphery | – | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 1 | 4 | 0 | 0 | 0 | 0 |
| Nucleolus | – | 17 | – | – | – | – | – | – | – | – | – | – | – | – | – | 29 | 20 | 10 | 0 | 5 | 0 | 0 | 0 | 0 |
| Peroxisomes | – | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
| SpindlePole | – | 2 | – | – | – | – | – | – | – | – | – | – | – | – | – | 25 | 22 | 7 | 3 | 25 | 0 | 0 | 0 | 0 |
| Vac/Vac Membrane | – | 39 | – | – | – | – | – | – | – | – | – | – | – | – | – | 131 | 119 | 56 | 3 | 21 | 0 | 0 | 0 | 3 |
| Unique Cell Count | 76 | 490 | 354 | 249 | 31 | 124 | 4 | 3 | 9 | 24 | ||||||||||||||
| Labelled Cell Count | 86 | 565 | 420 | 275 | 31 | 124 | 4 | 3 | 9 | 24 | ||||||||||||||
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | – | 27.2 | – | – | – | – | – | – | – | – | – | – | – | – | – | 27.4 | 31.4 | 32.4 | – | – | – |
| Std Deviation (1e-4) | – | 6.8 | – | – | – | – | – | – | – | – | – | – | – | – | – | 6.3 | 7.8 | 6.7 | – | – | – |
| Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
External localization resources
Images
Protein Concentration and Protein Localization Data
| R1 | R2 | R3 | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
| Concentration | 35.1231 | 51.8103 | 42.1289 | 44.3921 | 50.386 | 44.2456 | 34.7721 | 43.9227 | 42.8232 | 44.5877 | 43.6923 | 40.1673 | 33.5921 | 35.9382 | 35.3928 | 34.8946 | 33.286 | 33.7285 |
| Actin | 0.0235 | 0.002 | 0.0179 | 0.0022 | 0.001 | 0.0208 | 0.0219 | 0.0009 | 0.0092 | 0.0003 | 0.0009 | 0.0264 | 0.0008 | 0.0006 | 0.0035 | 0.0017 | 0.0014 | 0.0073 |
| Bud | 0.0009 | 0.0013 | 0.0011 | 0.0007 | 0.0002 | 0.0018 | 0.0012 | 0.0008 | 0.0013 | 0.0007 | 0.0002 | 0.0008 | 0.0004 | 0.0006 | 0.0006 | 0.0005 | 0.0002 | 0.0009 |
| Bud Neck | 0.0011 | 0.0026 | 0.0057 | 0.0021 | 0.0006 | 0.1085 | 0.0052 | 0.0047 | 0.0124 | 0.0025 | 0.005 | 0.0683 | 0.005 | 0.0028 | 0.0043 | 0.0042 | 0.0066 | 0.117 |
| Bud Periphery | 0.0011 | 0.0009 | 0.0017 | 0.0006 | 0.0001 | 0.003 | 0.0014 | 0.001 | 0.001 | 0.0005 | 0.0003 | 0.0021 | 0.0007 | 0.0006 | 0.001 | 0.0005 | 0.0003 | 0.0021 |
| Bud Site | 0.003 | 0.0071 | 0.004 | 0.0008 | 0.0002 | 0.0014 | 0.0326 | 0.0047 | 0.0047 | 0.0006 | 0.0003 | 0.0006 | 0.0016 | 0.0034 | 0.003 | 0.0009 | 0.0003 | 0.001 |
| Cell Periphery | 0.0008 | 0.0023 | 0.0046 | 0.0034 | 0.0002 | 0.0016 | 0.0052 | 0.005 | 0.0028 | 0.0012 | 0.0023 | 0.0056 | 0.0024 | 0.0029 | 0.0057 | 0.001 | 0.0003 | 0.0024 |
| Cytoplasm | 0.0499 | 0.0288 | 0.0508 | 0.0675 | 0.0196 | 0.0258 | 0.0775 | 0.033 | 0.0398 | 0.0467 | 0.0535 | 0.0323 | 0.0172 | 0.0295 | 0.01 | 0.0299 | 0.0435 | 0.0152 |
| Cytoplasmic Foci | 0.0596 | 0.0361 | 0.0395 | 0.0253 | 0.0464 | 0.0422 | 0.0082 | 0.0125 | 0.0141 | 0.0009 | 0.0009 | 0.0122 | 0.0164 | 0.0351 | 0.0212 | 0.0176 | 0.0439 | 0.0448 |
| Eisosomes | 0.0004 | 0.0001 | 0.0005 | 0.0003 | 0.0001 | 0.0002 | 0.0012 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 |
| Endoplasmic Reticulum | 0.0058 | 0.0012 | 0.0115 | 0.0095 | 0.0008 | 0.006 | 0.0069 | 0.0017 | 0.0013 | 0.0007 | 0.0074 | 0.0073 | 0.002 | 0.0013 | 0.0043 | 0.002 | 0.0035 | 0.0067 |
| Endosome | 0.0132 | 0.0024 | 0.0131 | 0.0093 | 0.0041 | 0.0098 | 0.0066 | 0.0009 | 0.0024 | 0.0023 | 0.007 | 0.0049 | 0.0031 | 0.0024 | 0.0038 | 0.0056 | 0.0044 | 0.0106 |
| Golgi | 0.0052 | 0.0007 | 0.0041 | 0.0008 | 0.0039 | 0.0042 | 0.0017 | 0.0003 | 0.0006 | 0.0001 | 0.0002 | 0.0021 | 0.0013 | 0.0007 | 0.0017 | 0.0069 | 0.0021 | 0.0049 |
| Lipid Particles | 0.0289 | 0.0153 | 0.0084 | 0.0222 | 0.0316 | 0.0303 | 0.0033 | 0.0098 | 0.011 | 0.0007 | 0.0005 | 0.0173 | 0.0173 | 0.0196 | 0.0307 | 0.0227 | 0.0381 | 0.0518 |
| Mitochondria | 0.0078 | 0.0016 | 0.0082 | 0.0043 | 0.0328 | 0.0032 | 0.0053 | 0.0006 | 0.0027 | 0.0003 | 0.0008 | 0.0039 | 0.0011 | 0.0005 | 0.001 | 0.0011 | 0.0004 | 0.001 |
| None | 0.0101 | 0.0006 | 0.0068 | 0.0065 | 0.0078 | 0.0038 | 0.0072 | 0.0003 | 0.0007 | 0.0003 | 0.0002 | 0.0009 | 0.0006 | 0.0003 | 0.0003 | 0.0004 | 0.0003 | 0.0004 |
| Nuclear Periphery | 0.0655 | 0.0264 | 0.0775 | 0.0786 | 0.1036 | 0.0759 | 0.0651 | 0.0424 | 0.0429 | 0.0334 | 0.094 | 0.091 | 0.101 | 0.0501 | 0.0988 | 0.1019 | 0.1397 | 0.0922 |
| Nucleolus | 0.1654 | 0.2943 | 0.189 | 0.2496 | 0.1239 | 0.1043 | 0.1846 | 0.2483 | 0.342 | 0.0888 | 0.1627 | 0.1253 | 0.1517 | 0.215 | 0.2419 | 0.1173 | 0.0391 | 0.1082 |
| Nucleus | 0.4053 | 0.4348 | 0.4541 | 0.427 | 0.4958 | 0.4096 | 0.4489 | 0.5536 | 0.4357 | 0.7811 | 0.5862 | 0.4326 | 0.563 | 0.4825 | 0.4529 | 0.476 | 0.4621 | 0.3492 |
| Peroxisomes | 0.0119 | 0.0004 | 0.0023 | 0.0004 | 0.0047 | 0.0014 | 0.0007 | 0.0002 | 0.0005 | 0 | 0 | 0.0003 | 0.0005 | 0.0003 | 0.0005 | 0.0004 | 0.0003 | 0.0011 |
| Punctate Nuclear | 0.1325 | 0.129 | 0.0764 | 0.0705 | 0.1174 | 0.1323 | 0.105 | 0.068 | 0.0614 | 0.0041 | 0.0644 | 0.1445 | 0.105 | 0.1373 | 0.0994 | 0.2045 | 0.2089 | 0.1701 |
| Vacuole | 0.0047 | 0.0107 | 0.0187 | 0.0121 | 0.0016 | 0.0103 | 0.0073 | 0.0101 | 0.0107 | 0.0255 | 0.0104 | 0.0177 | 0.0071 | 0.012 | 0.0125 | 0.0032 | 0.0031 | 0.0108 |
| Vacuole Periphery | 0.0034 | 0.0016 | 0.004 | 0.0063 | 0.0037 | 0.0038 | 0.0029 | 0.0011 | 0.0027 | 0.0095 | 0.0027 | 0.0037 | 0.0017 | 0.0025 | 0.0027 | 0.0014 | 0.0015 | 0.0023 |
Sequencing Data
| R1 | R2 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
| Gene Expression | 125.1465 | 136.559 | 120.8197 | 164.1101 | 123.4074 | 95.1292 | 139.4023 | 160.9338 | 142.3801 | 144.3759 |
| Translational Efficiency | 2.1153 | 2.0117 | 1.933 | 1.2856 | 1.595 | 2.6344 | 2.1454 | 1.3056 | 1.5478 | 1.5561 |
Hit Data
| Dataset | Hit |
|---|---|
| Protein Concentration | ✔ |
| Protein Localization | ✘ |
| Gene Expression | ✔ |
| Translational Efficiency | ✘ |
Micrographs
Select an organelle or subcellular structure:
Penetrance Data
Penetrance values (percentage of cells with morphologically abnormal structure) at each time point for each of the subcellular structures monitored.
| TP0 | TP3 | TP6 | TP9 | TP12 | TP15 | TP18 | TP21 | TP24 | |
|---|---|---|---|---|---|---|---|---|---|
| Autophagosome (ATG8) | 45.652174 | 90.277778 | 98.461538 | 98.529412 | 100.000000 | 89.361702 | 95.454545 | 94.444444 | 100.000000 |
| Septin (CDC11) | 33.027523 | 47.857143 | 76.608187 | 80.308880 | 87.969925 | 83.809524 | 90.084986 | 87.126437 | 77.657267 |
| Spindle & SPB (DAD2) | 41.666667 | 83.695652 | 86.111111 | 83.653846 | 78.888889 | 64.000000 | 64.948454 | 55.208333 | 62.500000 |
| P-bodies (DCP2) | 58.620690 | 74.468085 | 88.235294 | 98.461538 | 95.918367 | 100.000000 | 97.368421 | 97.709924 | 93.081761 |
| Inner Nuclear Membrane (HEH2) | 52.727273 | 77.611940 | 82.926829 | 89.000000 | 93.406593 | 100.000000 | 100.000000 | 100.000000 | 95.121951 |
| Metacaspase (MCA1) | 18.243243 | 69.930070 | 95.731707 | 90.196078 | 93.034826 | 88.829787 | 88.461538 | 76.190476 | 66.666667 |
| Plasma Membrane (MID2) | 61.864407 | 81.720430 | 95.000000 | 99.137931 | 99.186992 | 100.000000 | 100.000000 | 100.000000 | 99.270073 |
| Necrosis Factor (NHP6A) | 83.333333 | 86.666667 | 96.396396 | 94.244604 | 97.315436 | 99.438202 | 98.048780 | 96.610169 | 97.340426 |
| Nucleolus (NOP10) | 42.406877 | 48.286604 | 57.777778 | 59.965928 | 70.263789 | 60.114778 | 47.072464 | 33.668342 | 34.199584 |
| Kinetochore (NUF2) | 52.631579 | 61.904762 | 66.666667 | 74.096386 | 73.643411 | 82.758621 | 91.139241 | 91.379310 | 82.894737 |
| Mitochondria (OM45) | 37.500000 | 62.500000 | 85.585586 | 92.063492 | 100.000000 | 100.000000 | 100.000000 | 100.000000 | 100.000000 |
| Eisosomes (PIL1) | 38.181818 | 89.552239 | 97.297297 | 99.107143 | 96.568627 | 98.445596 | 100.000000 | 99.665552 | 99.298246 |
| Proteasome (PRE2) | 69.736842 | 49.618321 | 77.272727 | 78.787879 | 84.172662 | 94.852941 | 94.736842 | 87.248322 | 67.938931 |
| DNA Damage Foci (RAD52) | 48.543689 | 55.555556 | 64.788732 | 67.500000 | 78.915663 | 85.393258 | 89.032258 | 89.932886 | 91.095890 |
| Actin Patches (SAC6) | 60.294118 | 75.862069 | 94.117647 | 73.619632 | 93.181818 | 95.483871 | 100.000000 | 93.750000 | 78.378378 |
| Golgi, Early (SEC21) | 27.058824 | 46.268657 | 58.285714 | 63.387978 | 75.862069 | 90.476190 | 96.721311 | 93.452381 | 91.515152 |
| Golgi, Late (SEC7) | 32.954545 | 77.142857 | 83.200000 | 87.313433 | 100.000000 | 98.412698 | 99.242424 | 97.692308 | 93.859649 |
| Endosomes (SNF7) | 20.426829 | 17.868539 | 28.178593 | 36.325148 | 52.853380 | 62.473795 | 62.160531 | 66.798780 | 60.781955 |
| Endoplasmic Reticulum (SPF1) | 41.353383 | 41.566265 | 76.923077 | 78.010471 | 75.126904 | 88.118812 | 96.396396 | 94.666667 | 92.982456 |
| Lipid Droplets (TGL3) | 13.592233 | 15.115163 | 30.405688 | 44.749216 | 60.629026 | 72.795086 | 70.308337 | 69.743988 | 68.360277 |
| Vacuole (VPH1) | 55.555556 | 39.375000 | 68.899522 | 86.086957 | 97.202797 | 96.000000 | 92.592593 | 95.945946 | 98.583569 |
Micrographs
Cell Count
Number of cells analysed for each strain. R1, R2: biological replicates; R1 & R2: both replicates combined.
| R1 | R2 | R1 & R2 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
| 1454 | 826 | 3182 | 81 | 782 | 1447 | 1457 | 71 | 2236 | 2273 | 4639 | 152 |
Protein Abundance
Mean and standard deviation of cell GFP intensity, and intensity change (log2) of each mutant relative to wild type.
| R1 | R2 | R1 & R2 | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
| Mean | 2924.22 | 3088.37 | 4098.28 | 4936.19 | 3252.22 | 2984.15 | 3889.03 | 4638.85 | 3038.93 | 3022.02 | 4032.56 | 4797.30 |
| Standard Deviation | 851.64 | 995.07 | 1518.43 | 1839.47 | 795.18 | 922.59 | 1191.94 | 1487.96 | 846.90 | 950.89 | 1427.28 | 1690.95 |
| Intensity Change Log 2 | — | 0.078794 | 0.486967 | 0.755346 | — | -0.124105 | 0.257986 | 0.512342 | — | -0.024480 | 0.370934 | 0.632503 |
Localization Score (DeepLoc)
Localization score for each compartment. Values highlighted in green denote significant localization scores (marked * in the plot).
| R1 | R2 | R1 & R2 | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
| Actin | 0.005374 | 0.003343 | 0.001825 | 0.006803 | 0.004764 | 0.004381 | 0.002524 | 0.005006 | 0.005161 | 0.004003 | 0.002045 | 0.005964 |
| Bud Neck | 0.304856* | 0.221892* | 0.254321* | 0.142132 | 0.303458* | 0.301037* | 0.228761* | 0.091375 | 0.304367* | 0.272276* | 0.246293* | 0.118423 |
| Bud Site | 0.249826* | 0.120138* | 0.062943 | 0.208276* | 0.137208* | 0.167020* | 0.110787* | 0.290254* | 0.210440* | 0.149983* | 0.077970 | 0.246568* |
| Cell Periphery | 0.007942 | 0.002261 | 0.001041 | 0.034440 | 0.005296 | 0.003153 | 0.001172 | 0.006245 | 0.007017 | 0.002829 | 0.001082 | 0.021270 |
| Cytoplasm | 0.023252 | 0.030035 | 0.014980 | 0.059863 | 0.031565 | 0.033940 | 0.060150 | 0.023974 | 0.026159 | 0.032521 | 0.029167 | 0.043099 |
| Cytoplasmic Foci | 0.066801 | 0.090600 | 0.015399 | 0.206301* | 0.078402 | 0.043096 | 0.022078 | 0.238156* | 0.070858 | 0.060359 | 0.017497 | 0.221180* |
| Eisosomes | 0.000785 | 0.000258 | 0.000088 | 0.001663 | 0.000810 | 0.000308 | 0.000092 | 0.000528 | 0.000794 | 0.000290 | 0.000089 | 0.001133 |
| Endoplasmic Reticulum | 0.011713 | 0.002172 | 0.006742 | 0.000559 | 0.005081 | 0.007669 | 0.002490 | 0.002708 | 0.009394 | 0.005672 | 0.005406 | 0.001563 |
| Endosome | 0.005160 | 0.003620 | 0.006694 | 0.023452 | 0.001646 | 0.005281 | 0.003660 | 0.011629 | 0.003931 | 0.004678 | 0.005741 | 0.017929 |
| Golgi | 0.000932 | 0.001942 | 0.000948 | 0.002265 | 0.000671 | 0.001023 | 0.000836 | 0.003115 | 0.000840 | 0.001357 | 0.000913 | 0.002662 |
| Lipid Particles | 0.009497 | 0.004820 | 0.001969 | 0.037133 | 0.003928 | 0.003716 | 0.001543 | 0.007011 | 0.007550 | 0.004117 | 0.001835 | 0.023063 |
| Mitochondria | 0.005937 | 0.006222 | 0.015922 | 0.001215 | 0.006176 | 0.006951 | 0.008365 | 0.009964 | 0.006021 | 0.006686 | 0.013549 | 0.005302 |
| Mitotic Spindle | 0.066294 | 0.054577 | 0.215406* | 0.037571 | 0.021331 | 0.044788 | 0.155791* | 0.111260* | 0.050569 | 0.048346 | 0.196682* | 0.071991 |
| None | 0.003618 | 0.005207 | 0.003345 | 0.001175 | 0.005134 | 0.006492 | 0.013962 | 0.000435 | 0.004148 | 0.006025 | 0.006680 | 0.000829 |
| Nuclear Periphery | 0.003262 | 0.003729 | 0.025114 | 0.008627 | 0.001968 | 0.005514 | 0.008224 | 0.012856 | 0.002809 | 0.004866 | 0.019809 | 0.010602 |
| Nuclear Periphery Foci | 0.035268 | 0.024172 | 0.023158 | 0.015203 | 0.015000 | 0.019002 | 0.006222 | 0.017617 | 0.028180 | 0.020881 | 0.017839 | 0.016331 |
| Nucleolus | 0.018517 | 0.075067 | 0.136212 | 0.017523 | 0.027746 | 0.036630 | 0.061349 | 0.023745 | 0.021745 | 0.050598 | 0.112699 | 0.020429 |
| Nucleus | 0.153723* | 0.317695* | 0.199201* | 0.042359 | 0.326945* | 0.288992* | 0.291895* | 0.023199 | 0.214304* | 0.299422* | 0.228314* | 0.033409 |
| Peroxisomes | 0.005175 | 0.009489 | 0.002790 | 0.006233 | 0.003339 | 0.003629 | 0.002375 | 0.010908 | 0.004533 | 0.005759 | 0.002659 | 0.008417 |
| Vacuole | 0.020894 | 0.021280 | 0.009830 | 0.140534 | 0.018984 | 0.016082 | 0.016608 | 0.093394 | 0.020226 | 0.017971 | 0.011959 | 0.118515 |
| Vacuole Periphery | 0.001174 | 0.001482 | 0.002074 | 0.006673 | 0.000548 | 0.001294 | 0.001113 | 0.016621 | 0.000955 | 0.001363 | 0.001772 | 0.011320 |
Localization Changes (T-score)
Change in localization of each mutant relative to its control (e.g. UBP2_WT: ubp2Δ vs wild type). Values highlighted in yellow (increase) or blue (decrease) denote significant changes (marked * in the plot).
| R1 | R2 | R1 & R2 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| UBP2_ |
UBP14_ |
UBP2UBP14_ |
UBP2UBP14_ |
UBP2UBP14_ |
UBP2_ |
UBP14_ |
UBP2UBP14_ |
UBP2UBP14_ |
UBP2UBP14_ |
UBP2_ |
UBP14_ |
UBP2UBP14_ |
UBP2UBP14_ |
UBP2UBP14_ |
|
| Actin | 4.48 | 9.61 | 1.45 | -0.74 | -2.41 | 0.66 | 4.40 | 1.49 | 1.17 | 0.03 | 3.09 | 10.57 | 2.21 | 0.60 | -1.66 |
| Bud Neck | 4.34 | 0.78 | 9.06 | 6.12 | 9.07 | 0.75 | 6.98 | 8.65 | 8.70 | 5.02 | 2.32 | 4.62 | 12.51 | 11.17 | 10.75 |
| Bud Site | 13.53 | 25.09* | 2.34 | -3.27 | -5.72 | -2.00 | 4.37 | -1.93 | -1.36 | -3.13 | 9.77 | 23.60 | 0.73 | -2.83 | -6.41 |
| Cell Periphery | 9.17 | 11.87 | -1.16 | -2.27 | -2.52 | 4.17 | 8.79 | -0.53 | -1.60 | -2.48 | 9.43 | 14.25 | -1.15 | -2.61 | -3.18 |
| Cytoplasm | -1.59 | 4.06 | -1.85 | -1.48 | -2.44 | -2.10 | -4.69 | 0.58 | 1.81 | 3.50 | -3.20 | -0.29 | -1.51 | -0.62 | -1.46 |
| Cytoplasmic Foci | -1.77 | 11.75 | -4.51 | -4.02 | -6.35 | 6.32 | 9.69 | -4.30 | -6.04 | -6.78 | 4.30 | 15.23 | -6.37 | -7.39 | -9.28 |
| Eisosomes | 3.07 | 4.98 | 0.25 | -1.38 | -2.04 | 3.42 | 4.82 | 2.16 | -0.30 | -1.32 | 4.53 | 6.74 | 1.31 | -1.37 | -2.43 |
| Endoplasmic Reticulum | 9.59 | 5.59 | 11.23 | 7.16 | 14.82 | -2.91 | 7.22 | 7.14 | 8.22 | 2.83 | 4.61 | 6.84 | 12.62 | 9.95 | 12.21 |
| Endosome | 3.66 | -1.69 | -0.42 | -0.74 | -0.28 | -7.55 | -0.12 | -0.92 | 0.42 | -0.90 | -0.98 | -2.05 | -0.58 | -0.47 | -0.37 |
| Golgi | -3.59 | -0.99 | -0.40 | 1.39 | -0.21 | -1.20 | -0.67 | -1.53 | -1.25 | -1.43 | -2.93 | -1.30 | -1.33 | -0.30 | -1.10 |
| Lipid Particles | 4.66 | 9.77 | -2.93 | -3.33 | -3.55 | 0.78 | 2.15 | -3.17 | -3.31 | -3.49 | 5.70 | 9.53 | -3.81 | -4.35 | -4.59 |
| Mitochondria | -1.09 | -17.17 | 9.90 | 10.51 | 27.94* | -1.02 | -3.97 | 2.20 | 2.50 | 3.32 | -1.86 | -17.45 | 5.01 | 5.85 | 13.30 |
| Mitotic Spindle | 0.06 | -16.30 | 0.94 | 0.88 | 8.33 | -2.45 | -12.30 | -3.03 | -2.57 | 0.23 | 0.50 | -20.47 | -1.78 | -1.94 | 5.04 |
| None | -1.63 | 0.39 | 2.14 | 3.59 | 2.31 | -1.35 | -4.15 | 0.56 | 1.24 | 2.69 | -2.12 | -2.74 | 1.32 | 2.49 | 2.64 |
| Nuclear Periphery | -0.96 | -15.96 | -0.18 | 0.09 | 7.16 | -5.73 | -7.22 | -1.28 | -0.19 | 0.15 | -4.03 | -17.24 | -1.00 | 0.05 | 6.06 |
| Nuclear Periphery Foci | 4.02 | 5.43 | 6.04 | 2.71 | 2.98 | 0.40 | 6.60 | 3.05 | 2.89 | -1.25 | 4.32 | 7.25 | 7.06 | 3.85 | 2.58 |
| Nucleolus | -8.41 | -26.65* | 2.69 | 8.62 | 20.81* | -1.27 | -4.99 | 0.57 | 1.05 | 2.54 | -7.33 | -24.99* | 1.41 | 4.80 | 13.19 |
| Nucleus | -9.67 | -3.74 | 4.79 | 10.39 | 6.64 | -0.70 | -2.61 | 12.44 | 14.44 | 16.20 | -9.64 | -3.80 | 10.77 | 17.12 | 13.85 |
| Peroxisomes | -2.52 | 3.28 | 1.68 | 3.26 | 0.31 | 0.34 | 2.61 | -1.26 | -1.35 | -1.85 | -1.02 | 3.97 | 0.08 | 0.58 | -1.26 |
| Vacuole | -0.25 | 7.11 | -6.85 | -6.81 | -7.32 | -0.11 | -7.92 | -5.48 | -5.47 | -4.56 | 0.64 | 0.06 | -8.72 | -8.78 | -8.73 |
| Vacuole Periphery | -1.06 | -1.91 | -0.64 | -0.35 | -0.10 | -1.67 | -4.60 | -1.21 | -1.04 | -1.11 | -1.11 | -2.33 | -1.33 | -1.11 | -0.90 |
| Standard name | |
|---|---|
| Human Ortholog | |
| Description (SGD) | Type 1 S/T protein phosphatase (PP1) catalytic subunit; involved in glycogen metabolism, sporulation and mitotic progression; interacts with multiple regulatory subunits; regulates actomyosin ring formation; subunit of CPF; recruited to mating projections by Afr1p interaction; regulates nucleocytoplasmic shuttling of Hxk2p; import into the nucleus is inhibited during spindle assembly checkpoint arrest; involved in dephosphorylating Rps6a/b and Bnr1p |
| Localization | |
| Cell Percentages | cytoplasm (24%), nucleus (3%), mixed (56%) |
| Cell Cycle Regulation | No |
| Subcompartmental Group | N/A |
Glc7
Glc7
Nearest neighbours ( genes)
correlation threshold
0.80
Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)
Endocytosis
| Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
|---|---|---|---|---|
| 37℃ | ||||
| RT |
Cell Cycle Omics
CYCLoPs (Glc7-GFP)
| Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
|---|
| Gene | Images |
|---|
| Gene | Images |
|---|
Images are not yet available
Images are not yet available
