| Standard name | |
|---|---|
| Human Ortholog | |
| Description (SGD) | G protein alpha subunit involved in the pheromone response; GTP-binding subunit of the heterotrimeric G protein; negatively regulates mating pathway by sequestering G(beta)gamma and triggering an adaptive response; interacts with the Ste5p scaffold protein to enhance the efficiency of polarity site alignment; activates Vps34p at endosome; protein abundance increases in response to DNA replication stress; mutations in human homolog GNAS associated with McCune-Albright syndrome |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | – | – | 57 | 52 | 64 | 156 | 145 | 89 | – | 87 | 84 | 82 | – | 8 | 7 | – | – | – | – | 0 | 9 | 0 | 1 | 1 |
| Bud | – | – | 23 | 12 | 12 | 32 | 16 | 25 | – | 35 | 46 | 13 | – | 1 | 1 | – | – | – | – | 0 | 3 | 0 | 1 | 1 |
| Bud Neck | – | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | 0 | 8 | 3 | 0 | 0 |
| Bud Site | – | – | 0 | 0 | 2 | 5 | 2 | 0 | – | 2 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | – | 180 | 169 | 88 | 195 | 201 | 100 | – | 116 | 102 | 115 | – | 26 | 28 | – | – | – | – | 0 | 289 | 39 | 78 | 76 |
| Cytoplasm | – | – | 31 | 9 | 17 | 41 | 34 | 61 | – | 63 | 69 | 41 | – | 2 | 8 | – | – | – | – | 1 | 2 | 1 | 1 | 0 |
| Endoplasmic Reticulum | – | – | 9 | 15 | 8 | 25 | 26 | 27 | – | 29 | 14 | 27 | – | 0 | 1 | – | – | – | – | 0 | 10 | 0 | 2 | 1 |
| Endosome | – | – | 0 | 0 | 1 | 1 | 2 | 2 | – | 2 | 0 | 0 | – | 0 | 0 | – | – | – | – | 0 | 0 | 0 | 0 | 0 |
| Golgi | – | – | 0 | 0 | 0 | 9 | 1 | 2 | – | 1 | 0 | 1 | – | 0 | 0 | – | – | – | – | 0 | 0 | 0 | 0 | 0 |
| Mitochondria | – | – | 13 | 21 | 5 | 59 | 68 | 49 | – | 88 | 86 | 74 | – | 0 | 0 | – | – | – | – | 1 | 6 | 4 | 2 | 2 |
| Nucleus | – | – | 1 | 0 | 0 | 5 | 1 | 0 | – | 1 | 1 | 1 | – | 0 | 0 | – | – | – | – | 0 | 0 | 0 | 0 | 0 |
| Nuclear Periphery | – | – | 2 | 2 | 2 | 3 | 10 | 9 | – | 9 | 2 | 8 | – | 0 | 0 | – | – | – | – | 0 | 0 | 0 | 0 | 0 |
| Nucleolus | – | – | 0 | 0 | 0 | 1 | 0 | 5 | – | 0 | 1 | 0 | – | 0 | 0 | – | – | – | – | 0 | 0 | 0 | 0 | 0 |
| Peroxisomes | – | – | 3 | 1 | 2 | 2 | 2 | 1 | – | 3 | 0 | 2 | – | 0 | 0 | – | – | – | – | 0 | 0 | 0 | 0 | 0 |
| SpindlePole | – | – | 0 | 0 | 1 | 6 | 0 | 1 | – | 0 | 0 | 1 | – | 0 | 0 | – | – | – | – | 0 | 0 | 0 | 0 | 0 |
| Vac/Vac Membrane | – | – | 2 | 0 | 2 | 9 | 0 | 5 | – | 0 | 0 | 3 | – | 0 | 0 | – | – | – | – | 0 | 3 | 1 | 0 | 0 |
| Unique Cell Count | 253 | 218 | 151 | 348 | 319 | 265 | 275 | 261 | 244 | 27 | 32 | 4 | 338 | 53 | 89 | 83 | ||||||||
| Labelled Cell Count | 321 | 281 | 204 | 549 | 508 | 376 | 436 | 405 | 368 | 37 | 45 | 4 | 338 | 53 | 89 | 83 | ||||||||
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | – | – | 13.2 | 13.7 | 12.2 | 11.1 | 10.9 | 10.7 | – | 9.9 | 9.6 | 10.3 | – | 13.4 | 13.4 | – | – | – | 18.8 | 19.6 | 19.8 |
| Std Deviation (1e-4) | – | – | 2.9 | 2.9 | 2.5 | 2.4 | 2.3 | 2.4 | – | 2.4 | 2.3 | 2.6 | – | 2.3 | 2.2 | – | – | – | 3.0 | 3.1 | 3.2 |
| Intensity Change (Log2) | – | – | – | 0.06 | -0.11 | -0.24 | -0.27 | -0.29 | – | -0.42 | -0.45 | -0.35 | – | 0.02 | 0.02 | – | – | – | 0.51 | 0.57 | 0.59 |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Cortical Patches | 0.3 | 4.2 | 5.6 | 5.7 | 2.8 | – | 2.3 | 2.5 | 2.8 | – | 0.8 | -0.1 | – | – | – |
| Bud | -1.5 | -0.4 | 0 | -1.9 | 0.1 | – | 1.3 | 2.8 | -1.6 | – | 0 | 0 | – | – | – |
| Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | 0 | 0 | – | – | – |
| Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | 0 | 0 | – | – | – |
| Cell Periphery | 1.6 | -2.6 | -3.8 | -2.0 | -7.6 | – | -6.7 | -7.3 | -5.4 | – | 2.8 | 2.0 | – | – | – |
| Cytoplasm | -3.2 | -0.3 | -0.2 | -0.6 | 3.2 | – | 3.2 | 4.1 | 1.4 | – | 0 | 0 | – | – | – |
| Endoplasmic Reticulum | 1.6 | 0.8 | 1.9 | 2.3 | 3.0 | – | 3.1 | 1.0 | 3.2 | – | 0 | 0 | – | – | – |
| Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | 0 | 0 | – | – | – |
| Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | 0 | 0 | – | – | – |
| Mitochondria | 1.9 | -0.9 | 4.4 | 5.5 | 4.7 | – | 7.8 | 8.0 | 7.4 | – | 0 | 0 | – | – | – |
| Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | 0 | 0 | – | – | – |
| Nuclear Periphery | 0 | 0 | 0 | 1.9 | 2.1 | – | 2.0 | 0 | 0 | – | 0 | 0 | – | – | – |
| Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | 0 | 0 | – | – | – |
| Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | 0 | 0 | – | – | – |
| SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | 0 | 0 | – | – | – |
| Vacuole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | 0 | 0 | – | – | – |
External localization resources
Images
Protein Concentration and Protein Localization Data
| R1 | R2 | R3 | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
| Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasmic Foci | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Eisosomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Lipid Particles | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| None | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Punctate Nuclear | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Sequencing Data
| R1 | R2 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
| Gene Expression | 15.5519 | 15.9878 | 50.0335 | 77.9636 | 35.7905 | 18.6519 | 21.0851 | 31.7826 | 54.8469 | 33.0726 |
| Translational Efficiency | 0.8165 | 0.9154 | 1.0914 | 1.0807 | 1.01 | 1.0056 | 0.9288 | 0.8761 | 1.0757 | 1.0119 |
Hit Data
| Dataset | Hit |
|---|---|
| Protein Concentration | – |
| Protein Localization | – |
| Gene Expression | ✔ |
| Translational Efficiency | ✔ |
Micrographs
Cell Count
Number of cells analysed for each strain. R1, R2: biological replicates; R1 & R2: both replicates combined.
| R1 | R2 | R1 & R2 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
| N/A | 1275 | N/A | N/A | N/A | 1655 | 1057 | N/A | 0 | 2930 | 1057 | 0 |
Protein Abundance
Mean and standard deviation of cell GFP intensity, and intensity change (log2) of each mutant relative to wild type.
| R1 | R2 | R1 & R2 | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
| Mean | N/A | 1353.78 | N/A | N/A | N/A | 1243.65 | 1194.52 | N/A | — | 1291.57 | 1194.52 | — |
| Standard Deviation | N/A | 256.87 | N/A | N/A | N/A | 229.41 | 161.30 | N/A | — | 247.83 | 161.30 | — |
| Intensity Change Log 2 | N/A | — | N/A | N/A | N/A | — | — | N/A | — | — | — | — |
Localization Score (DeepLoc)
Localization score for each compartment. Values highlighted in green denote significant localization scores (marked * in the plot).
| R1 | R2 | R1 & R2 | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
| Actin | N/A | 0.003004 | N/A | N/A | N/A | 0.002164 | 0.032526 | N/A | N/A | 0.002529 | 0.032526 | N/A |
| Bud Neck | N/A | 0.011131 | N/A | N/A | N/A | 0.007658 | 0.056800 | N/A | N/A | 0.009169 | 0.056800 | N/A |
| Bud Site | N/A | 0.052111 | N/A | N/A | N/A | 0.057230 | 0.178270* | N/A | N/A | 0.055002 | 0.178270* | N/A |
| Cell Periphery | N/A | 0.814235* | N/A | N/A | N/A | 0.861959* | 0.499790* | N/A | N/A | 0.841192* | 0.499790* | N/A |
| Cytoplasm | N/A | 0.092649 | N/A | N/A | N/A | 0.049148 | 0.159543* | N/A | N/A | 0.068077 | 0.159543* | N/A |
| Cytoplasmic Foci | N/A | 0.002343 | N/A | N/A | N/A | 0.002278 | 0.003624 | N/A | N/A | 0.002307 | 0.003624 | N/A |
| Eisosomes | N/A | 0.000556 | N/A | N/A | N/A | 0.000406 | 0.011903 | N/A | N/A | 0.000471 | 0.011903 | N/A |
| Endoplasmic Reticulum | N/A | 0.004663 | N/A | N/A | N/A | 0.005168 | 0.010251 | N/A | N/A | 0.004948 | 0.010251 | N/A |
| Endosome | N/A | 0.001899 | N/A | N/A | N/A | 0.002178 | 0.002966 | N/A | N/A | 0.002057 | 0.002966 | N/A |
| Golgi | N/A | 0.001914 | N/A | N/A | N/A | 0.001464 | 0.002218 | N/A | N/A | 0.001660 | 0.002218 | N/A |
| Lipid Particles | N/A | 0.000980 | N/A | N/A | N/A | 0.000253 | 0.002253 | N/A | N/A | 0.000569 | 0.002253 | N/A |
| Mitochondria | N/A | 0.001122 | N/A | N/A | N/A | 0.002700 | 0.000692 | N/A | N/A | 0.002013 | 0.000692 | N/A |
| Mitotic Spindle | N/A | 0.000443 | N/A | N/A | N/A | 0.000222 | 0.000526 | N/A | N/A | 0.000318 | 0.000526 | N/A |
| None | N/A | 0.000038 | N/A | N/A | N/A | 0.000116 | 0.000020 | N/A | N/A | 0.000082 | 0.000020 | N/A |
| Nuclear Periphery | N/A | 0.000395 | N/A | N/A | N/A | 0.000245 | 0.000116 | N/A | N/A | 0.000310 | 0.000116 | N/A |
| Nuclear Periphery Foci | N/A | 0.000461 | N/A | N/A | N/A | 0.000167 | 0.000561 | N/A | N/A | 0.000295 | 0.000561 | N/A |
| Nucleolus | N/A | 0.000035 | N/A | N/A | N/A | 0.000041 | 0.000018 | N/A | N/A | 0.000038 | 0.000018 | N/A |
| Nucleus | N/A | 0.000334 | N/A | N/A | N/A | 0.000349 | 0.002786 | N/A | N/A | 0.000342 | 0.002786 | N/A |
| Peroxisomes | N/A | 0.000628 | N/A | N/A | N/A | 0.000766 | 0.000741 | N/A | N/A | 0.000706 | 0.000741 | N/A |
| Vacuole | N/A | 0.009175 | N/A | N/A | N/A | 0.003879 | 0.033388 | N/A | N/A | 0.006183 | 0.033388 | N/A |
| Vacuole Periphery | N/A | 0.001885 | N/A | N/A | N/A | 0.001611 | 0.001008 | N/A | N/A | 0.001730 | 0.001008 | N/A |
Localization Changes (T-score)
Change in localization of each mutant relative to its control (e.g. UBP2_WT: ubp2Δ vs wild type). Values highlighted in yellow (increase) or blue (decrease) denote significant changes (marked * in the plot).
| R1 | R2 | R1 & R2 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| UBP2_ |
UBP14_ |
UBP2UBP14_ |
UBP2UBP14_ |
UBP2UBP14_ |
UBP2_ |
UBP14_ |
UBP2UBP14_ |
UBP2UBP14_ |
UBP2UBP14_ |
UBP2_ |
UBP14_ |
UBP2UBP14_ |
UBP2UBP14_ |
UBP2UBP14_ |
|
| Actin | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Bud Neck | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Bud Site | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Cell Periphery | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Cytoplasm | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Cytoplasmic Foci | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Eisosomes | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Endoplasmic Reticulum | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Endosome | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Golgi | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Lipid Particles | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Mitochondria | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Mitotic Spindle | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| None | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Nuclear Periphery | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Nuclear Periphery Foci | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Nucleolus | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Nucleus | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Peroxisomes | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Vacuole | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
| Vacuole Periphery | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
Endocytosis
| Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
|---|---|---|---|---|
| 37℃ | ||||
| RT |
Cell Cycle Omics
CYCLoPs (Gpa1-GFP)
| Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
|---|
| Gene | Images |
|---|
| Gene | Images |
|---|
Images are not yet available
Images are not yet available
