Standard name | |
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Human Ortholog | |
Description | Cis-golgi GTPase-activating protein (GAP) for yeast Rabs; the Rab family members are Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 8 | 9 | 11 | 5 | 10 | 12 | 13 | – | 11 | 16 | 9 | 17 | 1 | 7 | 2 | 3 | 5 | 2 | 12 | 11 | 6 | 2 | 5 | 7 |
Bud | 3 | 2 | 0 | 1 | 0 | 4 | 13 | – | 9 | 9 | 12 | 16 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 4 |
Bud Neck | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 3 | 2 | 4 | 4 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 1 | 2 | 1 | 3 | 2 | 3 | – | 4 | 12 | 4 | 8 | 4 | 2 | 0 | 7 | 1 | 3 | 0 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 133 | 217 | 143 | 144 | 188 | 139 | 193 | – | 90 | 141 | 111 | 134 | 58 | 204 | 220 | 138 | 108 | 113 | 87 | 166 | 96 | 92 | 277 | 246 |
Endoplasmic Reticulum | 10 | 17 | 5 | 9 | 15 | 19 | 6 | – | 14 | 24 | 19 | 30 | 3 | 11 | 2 | 7 | 3 | 5 | 5 | 6 | 9 | 0 | 2 | 4 |
Endosome | 31 | 9 | 7 | 10 | 26 | 26 | 58 | – | 24 | 38 | 12 | 32 | 0 | 13 | 20 | 18 | 13 | 6 | 57 | 30 | 11 | 7 | 11 | 12 |
Golgi | 0 | 1 | 0 | 0 | 2 | 1 | 3 | – | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 2 | 0 | 2 | 11 | 5 | 4 | 0 | 1 | 1 |
Mitochondria | 11 | 4 | 0 | 7 | 3 | 33 | 61 | – | 63 | 84 | 172 | 118 | 0 | 0 | 0 | 3 | 1 | 2 | 27 | 40 | 51 | 16 | 19 | 22 |
Nucleus | 0 | 2 | 1 | 1 | 1 | 4 | 1 | – | 0 | 0 | 2 | 4 | 0 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 1 | 0 | 0 | 8 | – | 0 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 2 | 4 | 6 | 4 | 14 | – | 6 | 4 | 3 | 20 | 0 | 5 | 3 | 2 | 0 | 1 | 5 | 5 | 2 | 2 | 3 | 3 |
Vac/Vac Membrane | 8 | 5 | 6 | 1 | 7 | 6 | 20 | – | 9 | 18 | 8 | 21 | 0 | 4 | 10 | 21 | 20 | 12 | 9 | 19 | 12 | 4 | 11 | 5 |
Unique Cell Count | 191 | 255 | 168 | 172 | 245 | 223 | 345 | 194 | 299 | 288 | 331 | 61 | 237 | 251 | 172 | 136 | 130 | 226 | 296 | 209 | 135 | 349 | 319 | |
Labelled Cell Count | 207 | 268 | 178 | 184 | 261 | 250 | 394 | 231 | 350 | 355 | 405 | 67 | 248 | 257 | 203 | 153 | 146 | 226 | 296 | 209 | 135 | 349 | 319 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.2 | 6.6 | 7.2 | 6.7 | 7.5 | 6.4 | 6.6 | – | 6.0 | 6.5 | 5.5 | 5.9 | 6.9 | 6.9 | 6.9 | 9.6 | 9.7 | 9.1 | 6.3 | 6.7 | 7.0 |
Std Deviation (1e-4) | 0.7 | 0.9 | 1.1 | 1.9 | 1.5 | 1.1 | 1.4 | – | 1.2 | 1.7 | 1.0 | 1.3 | 0.9 | 0.9 | 1.2 | 2.4 | 2.4 | 2.1 | 0.9 | 1.0 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.1 | 0.04 | -0.18 | -0.13 | – | -0.26 | -0.16 | -0.4 | -0.3 | -0.06 | -0.06 | -0.08 | 0.41 | 0.42 | 0.33 | -0.2 | -0.12 | -0.05 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -1.6 | -1.1 | -0.5 | -1.4 | – | -0.3 | -0.5 | -1.7 | -0.6 | 0 | -1.7 | -3.3 | -2.2 | -1.1 | -2.1 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 1.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.4 | -2.1 | -5.0 | -6.5 | – | -7.7 | -8.1 | -9.7 | -9.5 | 2.0 | 0.3 | 0.7 | -1.2 | -1.3 | 0.4 |
Endoplasmic Reticulum | 1.0 | 1.5 | 2.3 | 0 | – | 1.8 | 2.2 | 1.7 | 2.5 | 0 | 0.8 | 0 | 0.5 | 0 | 0 |
Endosome | 0.7 | 2.4 | 2.6 | 4.0 | – | 2.8 | 3.0 | 0 | 2.2 | 0 | 0.6 | 1.6 | 2.2 | 1.9 | 0.2 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 5.2 | 5.8 | – | 8.1 | 7.6 | 12.7 | 8.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 1.8 | – | 0 | 0 | 0 | 2.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | -0.4 | -0.5 | 1.1 | – | 0.5 | 1.2 | -0.5 | 1.3 | 0 | 0 | 0.2 | 2.9 | 3.5 | 2.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.3521 | 0.6112 | 0.3857 | 0.1421 | -0.9705 | 0.3404 | 1.7031 | 2.1432 | 1.9442 | 1.8845 | 1.8172 | 1.8335 | 0.7684 | 0.6781 | 0.2492 | 1.0332 | 0.4227 | 0.6462 |
Actin | 0.0083 | 0.0085 | 0.0085 | 0.002 | 0.0024 | 0.0008 | 0.0228 | 0.0045 | 0.0412 | 0.0131 | 0.027 | 0.0108 | 0.0745 | 0.0012 | 0.0016 | 0.0508 | 0.0783 | 0.0144 |
Bud | 0.0006 | 0.002 | 0.0007 | 0.0003 | 0.0004 | 0.0001 | 0.0041 | 0.0009 | 0.0045 | 0.001 | 0.0008 | 0.0057 | 0.0006 | 0.0004 | 0.0004 | 0.0002 | 0.0005 | 0.0002 |
Bud Neck | 0.0052 | 0.0037 | 0.0061 | 0.0013 | 0.0002 | 0.0006 | 0.0021 | 0.0013 | 0.0032 | 0.0006 | 0.0007 | 0.0041 | 0.0007 | 0.0002 | 0.0002 | 0.0003 | 0.001 | 0.0009 |
Bud Periphery | 0.0008 | 0.0016 | 0.001 | 0.0006 | 0.0005 | 0.0001 | 0.0084 | 0.0008 | 0.0048 | 0.0012 | 0.0007 | 0.0028 | 0.0006 | 0.0002 | 0.0002 | 0.0002 | 0.0009 | 0.0003 |
Bud Site | 0.0084 | 0.0228 | 0.0028 | 0.0013 | 0.0015 | 0.0001 | 0.0125 | 0.0107 | 0.007 | 0.0021 | 0.0013 | 0.0005 | 0.002 | 0.0097 | 0.0006 | 0.0014 | 0.0015 | 0.0003 |
Cell Periphery | 0.0013 | 0.0006 | 0.0005 | 0.0012 | 0.0003 | 0.0001 | 0.0008 | 0.0003 | 0.0003 | 0.0004 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0003 | 0.0001 |
Cytoplasm | 0.2304 | 0.4443 | 0.4136 | 0.4076 | 0.2054 | 0.409 | 0.3244 | 0.5635 | 0.4544 | 0.3283 | 0.3788 | 0.5107 | 0.3209 | 0.6096 | 0.4725 | 0.2472 | 0.254 | 0.5203 |
Cytoplasmic Foci | 0.1419 | 0.0967 | 0.0707 | 0.1439 | 0.0848 | 0.1636 | 0.121 | 0.0818 | 0.1174 | 0.1561 | 0.1451 | 0.1191 | 0.072 | 0.0797 | 0.0908 | 0.0576 | 0.1469 | 0.1087 |
Eisosomes | 0.0015 | 0.0004 | 0.0011 | 0.0005 | 0.0003 | 0 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0008 | 0.0001 | 0.0001 | 0.0003 | 0.0013 | 0.0002 |
Endoplasmic Reticulum | 0.0095 | 0.0067 | 0.0076 | 0.0064 | 0.0087 | 0.0056 | 0.0141 | 0.0088 | 0.0067 | 0.0069 | 0.0088 | 0.009 | 0.0112 | 0.0062 | 0.0122 | 0.0176 | 0.0094 | 0.006 |
Endosome | 0.137 | 0.1222 | 0.1594 | 0.069 | 0.1297 | 0.1948 | 0.1318 | 0.0906 | 0.1394 | 0.2087 | 0.1861 | 0.1356 | 0.1594 | 0.1043 | 0.2221 | 0.1591 | 0.2141 | 0.1559 |
Golgi | 0.029 | 0.0115 | 0.0148 | 0.0067 | 0.0044 | 0.0162 | 0.0235 | 0.015 | 0.0244 | 0.0241 | 0.0444 | 0.0179 | 0.0177 | 0.0105 | 0.0132 | 0.0158 | 0.0535 | 0.0229 |
Lipid Particles | 0.042 | 0.0033 | 0.0105 | 0.0025 | 0.003 | 0.0072 | 0.0208 | 0.0059 | 0.0066 | 0.004 | 0.0097 | 0.003 | 0.015 | 0.0014 | 0.0022 | 0.0357 | 0.0889 | 0.0081 |
Mitochondria | 0.0119 | 0.0211 | 0.0095 | 0.0024 | 0.0025 | 0.003 | 0.0171 | 0.0149 | 0.0079 | 0.0075 | 0.0182 | 0.0148 | 0.0099 | 0.0041 | 0.0068 | 0.0036 | 0.0203 | 0.0057 |
None | 0.3127 | 0.2343 | 0.2643 | 0.3416 | 0.5435 | 0.1812 | 0.2181 | 0.1856 | 0.1455 | 0.2053 | 0.1459 | 0.151 | 0.2566 | 0.1588 | 0.1596 | 0.3795 | 0.0122 | 0.1437 |
Nuclear Periphery | 0.0031 | 0.0014 | 0.002 | 0.001 | 0.0011 | 0.0007 | 0.0184 | 0.0012 | 0.003 | 0.0038 | 0.002 | 0.0035 | 0.0162 | 0.0014 | 0.0024 | 0.0052 | 0.0152 | 0.0021 |
Nucleolus | 0.0048 | 0.0002 | 0.0004 | 0.0002 | 0.0003 | 0.0001 | 0.0012 | 0.0002 | 0.0014 | 0.0007 | 0.0001 | 0.0001 | 0.0013 | 0.0001 | 0.0001 | 0.0002 | 0.001 | 0.0001 |
Nucleus | 0.0028 | 0.001 | 0.0009 | 0.0013 | 0.0009 | 0.0006 | 0.017 | 0.0011 | 0.0092 | 0.0131 | 0.0011 | 0.0011 | 0.0063 | 0.0011 | 0.0008 | 0.0009 | 0.0022 | 0.0007 |
Peroxisomes | 0.0155 | 0.0077 | 0.0108 | 0.0018 | 0.0008 | 0.0059 | 0.0263 | 0.002 | 0.0103 | 0.0043 | 0.0176 | 0.0036 | 0.0088 | 0.0012 | 0.0009 | 0.0097 | 0.0453 | 0.0028 |
Punctate Nuclear | 0.0094 | 0.0012 | 0.0027 | 0.0014 | 0.0011 | 0.0009 | 0.0039 | 0.0007 | 0.0031 | 0.0052 | 0.0012 | 0.0009 | 0.0179 | 0.0008 | 0.0006 | 0.0028 | 0.0132 | 0.0012 |
Vacuole | 0.021 | 0.0067 | 0.0098 | 0.006 | 0.007 | 0.0074 | 0.0088 | 0.0086 | 0.0075 | 0.0112 | 0.0082 | 0.0039 | 0.0051 | 0.0071 | 0.0095 | 0.009 | 0.0347 | 0.0036 |
Vacuole Periphery | 0.0029 | 0.0022 | 0.0023 | 0.0008 | 0.0011 | 0.0021 | 0.0027 | 0.0015 | 0.0021 | 0.0024 | 0.0022 | 0.0016 | 0.0023 | 0.0013 | 0.003 | 0.0028 | 0.0058 | 0.0017 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.2593 | 11.3377 | 7.6482 | 11.8605 | 12.7066 | 12.6758 | 12.7662 | 12.8365 | 12.3915 | 14.3745 |
Translational Efficiency | 0.5576 | 0.8589 | 0.8923 | 0.6162 | 0.5806 | 0.9885 | 0.8873 | 0.8904 | 0.9307 | 0.6755 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
107 | 1015 | 2657 | 89 | 2565 | 1403 | 3163 | 190 | 2672 | 2418 | 5820 | 279 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 694.08 | 754.57 | 886.50 | 1005.37 | 797.15 | 768.59 | 974.64 | 926.48 | 793.02 | 762.70 | 934.40 | 951.65 |
Standard Deviation | 94.07 | 130.50 | 102.69 | 115.74 | 85.46 | 106.93 | 116.68 | 99.97 | 88.17 | 117.60 | 118.91 | 111.49 |
Intensity Change Log 2 | — | 0.120553 | 0.353019 | 0.534553 | — | -0.052637 | 0.290018 | 0.216909 | — | 0.030565 | 0.319684 | 0.373480 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001624 | 0.000905 | 0.000425 | 0.001676 | 0.000364 | 0.000836 | 0.000342 | 0.003330 | 0.000414 | 0.000865 | 0.000380 | 0.002802 |
Bud Neck | 0.023879 | 0.012182 | 0.014466 | 0.004771 | 0.012092 | 0.014993 | 0.009025 | 0.006279 | 0.012564 | 0.013813 | 0.011509 | 0.005798 |
Bud Site | 0.028607 | 0.028291 | 0.015530 | 0.039358 | 0.008767 | 0.023087 | 0.018348 | 0.030829 | 0.009562 | 0.025271 | 0.017061 | 0.033550 |
Cell Periphery | 0.000230 | 0.000107 | 0.000042 | 0.000167 | 0.000069 | 0.000109 | 0.000033 | 0.000117 | 0.000075 | 0.000108 | 0.000037 | 0.000133 |
Cytoplasm | 0.155394* | 0.157209* | 0.570846* | 0.169023* | 0.224340* | 0.173598* | 0.600851* | 0.196764* | 0.221579* | 0.166719* | 0.587153* | 0.187915* |
Cytoplasmic Foci | 0.495458* | 0.457739* | 0.141859 | 0.183017* | 0.452872* | 0.390542* | 0.097256 | 0.270044* | 0.454577* | 0.418749* | 0.117618 | 0.242283* |
Eisosomes | 0.000484 | 0.000128 | 0.000024 | 0.000096 | 0.000190 | 0.000117 | 0.000018 | 0.000094 | 0.000201 | 0.000122 | 0.000021 | 0.000095 |
Endoplasmic Reticulum | 0.002885 | 0.001723 | 0.000950 | 0.003524 | 0.003193 | 0.001140 | 0.000339 | 0.002450 | 0.003181 | 0.001385 | 0.000618 | 0.002792 |
Endosome | 0.083746 | 0.123740 | 0.018031 | 0.032350 | 0.072482 | 0.148359 | 0.018139 | 0.099873 | 0.072933 | 0.138025 | 0.018090 | 0.078333 |
Golgi | 0.051649 | 0.068971 | 0.002925 | 0.007355 | 0.064924 | 0.058240 | 0.001388 | 0.005403 | 0.064392 | 0.062745 | 0.002090 | 0.006025 |
Lipid Particles | 0.025763 | 0.013271 | 0.002831 | 0.003210 | 0.018238 | 0.009402 | 0.002069 | 0.008787 | 0.018540 | 0.011026 | 0.002417 | 0.007008 |
Mitochondria | 0.029351 | 0.016925 | 0.003019 | 0.000051 | 0.018530 | 0.021772 | 0.002001 | 0.000198 | 0.018963 | 0.019738 | 0.002465 | 0.000151 |
Mitotic Spindle | 0.000901 | 0.012495 | 0.047609 | 0.013915 | 0.002565 | 0.013771 | 0.040841 | 0.067257 | 0.002498 | 0.013235 | 0.043931 | 0.050241 |
None | 0.000177 | 0.002195 | 0.002434 | 0.000204 | 0.000555 | 0.001246 | 0.000959 | 0.000272 | 0.000540 | 0.001645 | 0.001632 | 0.000250 |
Nuclear Periphery | 0.000132 | 0.000325 | 0.001018 | 0.000402 | 0.000173 | 0.000251 | 0.000561 | 0.000732 | 0.000171 | 0.000282 | 0.000770 | 0.000627 |
Nuclear Periphery Foci | 0.000517 | 0.001453 | 0.001372 | 0.003816 | 0.000484 | 0.000459 | 0.000457 | 0.008753 | 0.000485 | 0.000876 | 0.000875 | 0.007178 |
Nucleolus | 0.000230 | 0.001616 | 0.001404 | 0.000092 | 0.000520 | 0.001405 | 0.000555 | 0.000700 | 0.000508 | 0.001493 | 0.000943 | 0.000506 |
Nucleus | 0.008331 | 0.007277 | 0.086571 | 0.040557 | 0.012151 | 0.007193 | 0.027190 | 0.019661 | 0.011998 | 0.007228 | 0.054299 | 0.026327 |
Peroxisomes | 0.024919 | 0.019797 | 0.008704 | 0.031090 | 0.009048 | 0.016379 | 0.005996 | 0.047779 | 0.009684 | 0.017814 | 0.007232 | 0.042455 |
Vacuole | 0.058467 | 0.064553 | 0.078884 | 0.465091* | 0.089793 | 0.104017 | 0.172352 | 0.229810* | 0.088539 | 0.087452 | 0.129681 | 0.304864* |
Vacuole Periphery | 0.007260 | 0.009097 | 0.001057 | 0.000234 | 0.008651 | 0.013082 | 0.001279 | 0.000867 | 0.008595 | 0.011409 | 0.001178 | 0.000665 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.75 | 1.27 | 0.81 | 0.26 | -1.33 | -2.58 | 1.35 | -5.33 | -4.04 | -5.48 | -3.73 | 0.97 | -5.30 | -3.79 | -5.50 |
Bud Neck | 1.75 | 1.32 | 3.19 | 8.59 | 12.75 | -2.33 | 5.76 | 6.24 | 6.42 | 1.83 | -1.28 | 1.75 | 9.14 | 9.20 | 8.94 |
Bud Site | 0.02 | 1.45 | 1.37 | 3.47 | -0.04 | -8.71 | -4.14 | -5.43 | -1.37 | -4.36 | -11.32 | -4.51 | -5.28 | 0.38 | -3.86 |
Cell Periphery | 2.34 | 3.72 | 3.16 | 2.22 | -1.44 | -2.61 | 15.46 | -4.55 | -0.66 | -8.10 | -3.07 | 12.56 | -2.89 | 0.37 | -7.36 |
Cytoplasm | -0.20 | -24.11 | -1.81 | -1.94 | 14.03 | 6.67 | -47.83 | 1.15 | -1.56 | 19.43 | 8.73 | -56.52 | 1.16 | -2.48 | 23.96 |
Cytoplasmic Foci | 1.70 | 17.14 | 18.16 | 28.27 | 5.95 | 8.54 | 77.48* | 15.93 | 10.91 | -10.40 | 5.97 | 74.50 | 23.88 | 20.35 | -5.94 |
Eisosomes | 3.11 | 4.02 | 3.80 | 7.70 | -3.54 | 5.48 | 19.67 | 7.76 | 1.46 | -9.90 | 6.86 | 18.79 | 10.50 | 4.33 | -10.08 |
Endoplasmic Reticulum | 0.82 | 1.30 | 1.23 | 1.15 | 0.02 | 5.23 | 8.64 | 0.31 | -4.28 | -6.91 | 4.98 | 7.78 | 1.74 | -2.91 | -5.52 |
Endosome | -4.06 | 7.29 | 7.74 | 21.65 | 2.33 | -15.61 | 30.32 | -0.32 | 7.15 | -6.87 | -17.64 | 30.45 | 2.68 | 11.43 | -6.09 |
Golgi | -1.89 | 5.77 | 5.94 | 18.94 | 2.00 | 2.24 | 31.75 | 25.06 | 18.27 | -1.93 | 0.82 | 31.87 | 28.07 | 25.57 | -1.08 |
Lipid Particles | 2.05 | 3.91 | 4.01 | 8.59 | 1.58 | 7.52 | 17.87 | 7.98 | 0.77 | -7.87 | 6.62 | 17.84 | 11.16 | 5.01 | -6.77 |
Mitochondria | 1.81 | 4.02 | 4.45 | 9.26 | 13.84 | -1.48 | 16.66 | 18.27 | 11.94 | 4.99 | -0.37 | 16.74 | 18.84 | 15.08 | 11.93 |
Mitotic Spindle | -6.20 | -17.31 | -1.67 | -0.54 | 2.86 | -5.93 | -15.05 | -5.64 | -4.59 | -2.58 | -7.77 | -22.15 | -5.72 | -4.41 | -1.05 |
None | -2.93 | -11.36 | -2.43 | 2.24 | 6.81 | -1.72 | -2.72 | 1.65 | 2.35 | 4.78 | -2.90 | -6.84 | 0.86 | 3.30 | 9.20 |
Nuclear Periphery | -2.23 | -13.92 | -6.34 | -3.52 | 3.44 | -2.34 | -14.87 | -6.97 | -6.33 | -0.64 | -2.68 | -19.81 | -8.43 | -6.97 | 0.69 |
Nuclear Periphery Foci | -2.36 | -4.05 | -4.86 | -2.39 | -2.46 | 0.13 | -0.40 | -5.15 | -5.17 | -5.13 | -1.86 | -3.16 | -5.54 | -5.27 | -5.18 |
Nucleolus | -3.06 | -11.79 | 2.00 | 3.21 | 13.07 | -3.34 | -2.64 | -1.95 | 1.83 | -0.65 | -3.92 | -7.91 | -0.92 | 3.40 | 4.48 |
Nucleus | 0.28 | -25.07 | -2.15 | -2.37 | 10.92 | 4.05 | -23.01 | -2.69 | -3.69 | 3.82 | 5.02 | -36.39 | -3.18 | -4.39 | 10.79 |
Peroxisomes | 1.12 | 3.80 | 2.26 | 1.91 | -0.29 | -7.04 | 7.83 | -4.43 | -3.15 | -5.24 | -8.93 | 5.75 | -3.99 | -2.10 | -4.78 |
Vacuole | -0.04 | -2.19 | -18.15 | -19.76 | -18.83 | -3.24 | -30.58 | -10.90 | -9.93 | -3.22 | 0.04 | -22.58 | -17.09 | -17.05 | -12.63 |
Vacuole Periphery | -1.26 | 4.82 | 5.50 | 13.21 | 12.31 | -4.59 | 18.01 | 18.91 | 14.37 | 0.44 | -3.92 | 19.06 | 20.13 | 18.84 | 3.11 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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