Standard name
Human Ortholog
Description (SGD) Trimeric heat shock transcription factor; forms condensate with Mediator and RNA Pol II upon heat shock; activates multiple genes in response to diverse stresses and transient intracellular acidification; recognizes variable heat shock elements (HSEs) consisting of inverted NGAAN repeats; monitors translational status through RQC (Ribosomal Quality Control)-mediated translation-stress signal; human homolog HSF1 with linker region mutations can complement yeast hsf1 mutant
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0.05 0 0.07 – 0.07 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.09 0.07 0.08 0.06 0.07 0.05 – 0.09 0.05 0 0.1 0.09 0.15 0.07 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.09 0.09 0.09 0.11 0.08 0.26 0.31 0.25 0.59 – 0.54 0.39 0.22 0 0 0 0 0.07 0 0 0 0 0 0
Nucleus 0.95 0.89 0.94 0.89 0.88 0.81 0.85 0.82 0.79 – 0.75 0.82 0.94 0.87 0.86 0.78 0.81 0.8 0.93 0.88 0.84 0.63 0.65 0.55
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0.07 – 0.11 0.06 0.11 0 0 0 0.1 0.05 0 0 0 0.16 0.15 0.18
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0.05 0 – 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0 0.05 0.06 0.09 0.11 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud 0 1 0 0 2 1 6 1 10 – 2 8 0 0 0 0 0 0 1 3 2 2 5 0
Bud Neck 0 0 2 2 1 1 1 3 0 – 1 5 0 0 0 0 0 0 0 0 1 1 2 1
Bud Site 0 0 2 4 3 6 18 6 19 – 8 12 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 7 9 16 16 23 22 23 13 – 10 10 5 34 29 10 5 5 0 3 5 0 0 1
Endoplasmic Reticulum 0 1 0 0 0 0 0 0 0 – 0 0 0 1 0 5 1 1 0 0 1 2 0 1
Endosome 0 1 2 1 1 1 1 2 0 – 0 0 0 1 1 0 0 1 1 1 1 3 4 10
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 2 0 1 0 0 0 0 0 0
Mitochondria 16 22 30 19 20 78 119 88 155 – 61 85 26 8 12 3 3 4 0 2 4 1 2 4
Nucleus 166 211 318 158 209 247 328 289 207 – 86 180 109 301 263 52 59 47 164 219 296 88 164 121
Nuclear Periphery 0 3 2 1 2 0 3 7 6 – 1 0 0 1 3 0 1 0 0 0 0 0 0 0
Nucleolus 4 3 4 3 4 6 16 9 18 – 12 13 13 6 8 1 7 3 0 3 14 22 36 39
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 2 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 13 5 4 8 4 16 3 – 6 6 1 1 2 0 1 1 1 1 1 4 1 9
Vac/Vac Membrane 2 7 2 1 0 3 1 1 0 – 1 2 1 5 5 0 4 1 5 11 20 12 27 23
Unique Cell Count 174 236 337 177 237 305 385 353 261 114 220 116 346 306 67 73 59 176 250 354 141 252 221
Labelled Cell Count 191 256 384 210 262 374 519 445 431 188 321 155 360 323 73 81 64 176 250 354 141 252 221


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 5.8 5.6 5.3 5.8 4.8 5.0 5.0 4.2 – 4.1 4.1 5.5 5.7 5.7 7.7 9.0 8.5 5.1 5.4 5.7
Std Deviation (1e-4) 0.7 1.3 0.9 1.5 1.5 1.3 1.5 1.3 1.5 – 1.5 0.9 1.1 1.2 1.6 1.4 2.0 2.0 1.1 1.1 1.5
Intensity Change (Log2) – – – -0.08 0.05 -0.22 -0.17 -0.16 -0.41 – -0.44 -0.44 -0.03 0.02 0.02 0.47 0.68 0.6 -0.13 -0.05 0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 3.3 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 3.2 2.4 2.8 2.0 2.4 1.5 – 0 1.2 0 3.8 3.7 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.7 -0.2 0 7.3 5.6 0 – 0 0 3.8 -3.8 -2.6 -1.2 -1.4 -0.5
Nucleus -2.1 -2.7 -5.2 -4.0 -5.0 -5.6 – -5.7 -4.7 -0.2 -3.3 -3.6 -4.5 -3.9 -3.9
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 2.4 1.3 0 – 0 0 0 0 1.3 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole -0.6 -1.5 0 -2.5 0.4 0 – 0 0 0 -3.3 -2.7 0 0 0
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.1314 3.9814 3.3048 3.2047 2.3818 3.1349 2.1364 2.3468 1.9486 1.8091 2.0213 1.8786 1.4904 1.0863 1.3538 0.9621 2.0994 1.0055
Actin 0.0293 0 0.0141 0 0.0589 0.0001 0.0094 0.0002 0.004 0.0039 0.0081 0.0052 0.0072 0 0.0108 0.0234 0.0322 0.0059
Bud 0.0003 0 0.0001 0 0.0001 0 0.0006 0 0.0004 0.0006 0.0019 0.0004 0.0001 0 0.0003 0.0006 0.0002 0.0002
Bud Neck 0.0023 0 0.0005 0 0.0003 0.0008 0.0021 0.0001 0.0006 0.0004 0.0013 0.0011 0.0003 0.0001 0.0004 0.0004 0.0009 0.0008
Bud Periphery 0.0006 0 0.0001 0 0.0002 0.0001 0.0014 0 0.0007 0.0011 0.0081 0.0009 0.0002 0 0.0002 0.0007 0.0003 0.0006
Bud Site 0.0038 0 0.0009 0 0.0003 0 0.0026 0.0001 0.0033 0.002 0.0044 0.0013 0.0004 0 0.0031 0.0052 0.001 0.0004
Cell Periphery 0.0005 0 0 0 0 0 0.0003 0 0.0004 0.0001 0.0007 0.0001 0.0001 0 0.0001 0.0001 0.0001 0.0001
Cytoplasm 0.0035 0.0001 0.0015 0 0.0002 0.0002 0.0086 0.0163 0.0028 0.0007 0.0004 0.0015 0.0161 0.0002 0.0028 0.0006 0.0005 0.0001
Cytoplasmic Foci 0.0077 0 0.0069 0 0.001 0.0001 0.0157 0.0011 0.0111 0.0039 0.01 0.0008 0.0125 0 0.0122 0.0039 0.0071 0.0009
Eisosomes 0.0012 0 0.0002 0 0.0002 0.0001 0.0002 0 0.0001 0 0.0001 0.0001 0.0001 0 0.0001 0.0002 0.0003 0.0001
Endoplasmic Reticulum 0.0026 0 0.0004 0 0.0002 0 0.0024 0.001 0.002 0.0005 0.0006 0.0007 0.0102 0 0.0005 0.0008 0.0006 0.0001
Endosome 0.0162 0 0.0045 0 0.0004 0.0002 0.0238 0.0016 0.0174 0.0377 0.0171 0.0023 0.0179 0 0.0035 0.0118 0.007 0.0022
Golgi 0.0075 0 0.0039 0 0.001 0.0001 0.0059 0.0001 0.011 0.0047 0.0099 0.0011 0.0039 0 0.0032 0.0074 0.0068 0.0035
Lipid Particles 0.0128 0 0.0092 0 0.0006 0.002 0.0219 0.0003 0.0062 0.0051 0.0401 0.0011 0.0075 0 0.0014 0.0047 0.0335 0.0051
Mitochondria 0.0063 0.0001 0.0073 0 0.0005 0.0074 0.0101 0.0002 0.0298 0.0038 0.0505 0.0092 0.0041 0.0001 0.0034 0.0043 0.0189 0.0168
None 0.0049 0 0.0049 0 0.0008 0.0089 0.0133 0.0025 0.0003 0.0004 0.0003 0.0002 0.0104 0.0001 0.0003 0.0006 0.0018 0
Nuclear Periphery 0.0037 0.0012 0.0028 0.0002 0.0054 0.0005 0.0236 0.0023 0.0046 0.0065 0.0065 0.0072 0.0093 0.0004 0.0012 0.0015 0.0017 0.0002
Nucleolus 0.0124 0.0084 0.0075 0.0047 0.0296 0.0479 0.0205 0.0118 0.0101 0.0065 0.051 0.032 0.0112 0.012 0.0071 0.0129 0.0255 0.0328
Nucleus 0.8648 0.9899 0.9187 0.9948 0.8933 0.9291 0.818 0.9601 0.882 0.9109 0.7366 0.9323 0.8731 0.9867 0.9437 0.916 0.8394 0.9277
Peroxisomes 0.0089 0 0.0068 0 0.0023 0.0004 0.0044 0.0001 0.0075 0.0021 0.0093 0.0007 0.0029 0 0.0048 0.0029 0.0153 0.0011
Punctate Nuclear 0.0057 0.0002 0.0091 0.0002 0.0045 0.0017 0.0076 0.0016 0.0008 0.0058 0.0042 0.0009 0.0048 0.0003 0.0009 0.0012 0.0063 0.0004
Vacuole 0.0035 0.0001 0.0003 0 0.0001 0.0001 0.0051 0.0004 0.0038 0.0021 0.0206 0.0006 0.0055 0 0.0002 0.0005 0.0003 0.0005
Vacuole Periphery 0.0015 0 0.0002 0 0.0001 0.0001 0.0024 0.0001 0.0009 0.0011 0.0183 0.0005 0.0021 0 0.0001 0.0004 0.0004 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.9943 12.6155 14.9195 22.9602 12.2934 15.8675 22.7208 23.6751 22.5397 21.5236
Translational Efficiency 0.9153 1.1573 0.9989 0.5855 1.0337 1.1701 0.709 0.7449 0.8326 0.7742

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Select an organelle or subcellular structure:


Penetrance Data

Penetrance values (percentage of cells with morphologically abnormal structure) at each time point for each of the subcellular structures monitored.

TP0 TP3 TP6 TP9 TP12 TP15 TP18 TP21 TP24
Autophagosome (ATG8) 15.662651 24.855491 33.037300 49.371634 68.181818 78.723404 88.621444 77.537797 81.617647
Septin (CDC11) 18.097448 22.684310 27.188082 51.479290 83.398438 92.727273 81.389578 75.876289 59.067358
Spindle & SPB (DAD2) 49.090909 34.319527 46.524064 57.396450 62.500000 79.738562 89.795918 81.927711 90.625000
P-bodies (DCP2) 38.823529 37.837838 49.612403 63.265306 96.385542 84.410646 89.928058 77.639752 78.688525
Inner Nuclear Membrane (HEH2) 29.607251 53.954802 85.148515 94.801223 97.229917 96.326531 96.464646 91.525424 91.821561
Metacaspase (MCA1) 8.739496 85.233161 71.137725 67.535545 69.095182 65.763195 63.118441 57.301587 51.703407
Plasma Membrane (MID2) 40.178571 29.387187 33.560709 60.869565 74.829932 89.756098 79.616307 83.585859 85.714286
Necrosis Factor (NHP6A) 58.139535 39.915966 59.205776 85.462555 90.794979 96.256684 90.384615 80.838323 80.676329
Nucleolus (NOP10) 27.941176 42.050520 70.151515 84.973166 94.382022 97.582418 97.428571 96.875000 98.986486
Kinetochore (NUF2) 33.128834 45.248869 39.634146 76.470588 68.750000 68.558952 69.696970 78.260870 70.661157
Mitochondria (OM45) 28.260870 28.301887 51.601423 68.768769 84.269663 99.145299 96.357616 94.791667 92.732558
Eisosomes (PIL1) 10.599078 47.488584 59.139785 82.978723 76.323988 100.000000 100.000000 98.936170 100.000000
Proteasome (PRE2) 40.975610 24.657534 39.130435 63.802559 88.321168 92.109777 91.131498 92.987805 83.397190
DNA Damage Foci (RAD52) 56.617647 34.730539 68.531469 78.181818 91.071429 96.330275 96.842105 95.597484 100.000000
Actin Patches (SAC6) 43.312102 53.409091 40.740741 80.851064 96.190476 100.000000 100.000000 100.000000 98.113208
Golgi, Early (SEC21) 19.205298 25.853659 33.333333 45.497630 62.553191 96.808511 98.550725 98.039216 92.792793
Golgi, Late (SEC7) 33.590734 49.300699 57.003257 62.876254 82.448980 86.826347 79.885057 70.000000 54.255319
Endosomes (SNF7) 86.206897 94.202899 86.904762 87.500000 77.272727 86.111111 85.897436 83.750000 83.116883
Endoplasmic Reticulum (SPF1) 39.516129 29.687500 32.707775 42.780749 51.058201 63.200000 71.070615 53.526971 53.280318
Lipid Droplets (TGL3) 14.238953 17.822266 33.036707 34.768534 38.786828 71.292373 55.655356 62.552777 67.005576
Vacuole (VPH1) 26.232742 26.702997 50.839329 81.098340 91.721419 93.610698 89.513678 91.808346 95.918367

Micrographs

Cell Count

Number of cells analysed for each strain. R1, R2: biological replicates; R1 & R2: both replicates combined.

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2653 1226 166 1487 1731 1773 529 2171 4384 2999 695 3658

Protein Abundance

Mean and standard deviation of cell GFP intensity, and intensity change (log2) of each mutant relative to wild type.

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 705.97 821.64 1079.34 966.86 719.01 808.97 883.75 994.81 711.12 814.15 930.47 983.45
Standard Deviation 79.14 115.65 132.57 138.55 85.73 105.79 142.90 138.68 82.05 110.10 163.38 139.30
Intensity Change Log 2 — 0.218900 0.612471 0.453700 — 0.170074 0.297626 0.468409 — 0.194470 0.462185 0.461141

Localization Score (DeepLoc)

Localization score for each compartment. Values highlighted in green denote significant localization scores (marked * in the plot).

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000075 0.000609 0.001873 0.001582 0.000236 0.000305 0.000535 0.000958 0.000139 0.000429 0.000854 0.001212
Bud Neck 0.003889 0.007620 0.007643 0.024024 0.003757 0.005266 0.004435 0.027232 0.003837 0.006228 0.005201 0.025928
Bud Site 0.001203 0.002045 0.001331 0.004987 0.000458 0.001420 0.000865 0.004332 0.000909 0.001676 0.000976 0.004598
Cell Periphery 0.000143 0.000071 0.000062 0.000070 0.000072 0.000120 0.000144 0.000217 0.000115 0.000100 0.000125 0.000157
Cytoplasm 0.020249 0.004530 0.000961 0.003690 0.011244 0.004380 0.009842 0.004395 0.016693 0.004441 0.007720 0.004108
Cytoplasmic Foci 0.001859 0.001689 0.000110 0.001777 0.001034 0.002116 0.001897 0.001110 0.001534 0.001942 0.001470 0.001381
Eisosomes 0.000010 0.000024 0.000074 0.000029 0.000016 0.000021 0.000037 0.000026 0.000013 0.000022 0.000046 0.000027
Endoplasmic Reticulum 0.001662 0.005277 0.010744 0.006464 0.002141 0.004708 0.003239 0.005636 0.001852 0.004940 0.005032 0.005973
Endosome 0.000497 0.000983 0.000347 0.002037 0.000583 0.001411 0.000412 0.001745 0.000531 0.001236 0.000396 0.001863
Golgi 0.000177 0.001162 0.000159 0.004467 0.000232 0.001516 0.000297 0.001938 0.000199 0.001372 0.000264 0.002966
Lipid Particles 0.000489 0.001066 0.000445 0.000269 0.000826 0.000968 0.000444 0.000098 0.000622 0.001008 0.000444 0.000168
Mitochondria 0.001341 0.003528 0.001458 0.002979 0.001572 0.005197 0.002110 0.003025 0.001432 0.004515 0.001955 0.003006
Mitotic Spindle 0.001292 0.003743 0.000820 0.015552 0.003634 0.005449 0.003397 0.013594 0.002217 0.004751 0.002781 0.014390
None 0.006423 0.005452 0.002143 0.003967 0.009775 0.004294 0.016635 0.003519 0.007746 0.004767 0.013174 0.003701
Nuclear Periphery 0.001274 0.000556 0.001625 0.001613 0.000624 0.000968 0.000329 0.002185 0.001017 0.000799 0.000639 0.001952
Nuclear Periphery Foci 0.000315 0.000932 0.000529 0.001059 0.001493 0.000426 0.000135 0.000675 0.000780 0.000632 0.000229 0.000831
Nucleolus 0.005169 0.023079 0.025109 0.006907 0.022982 0.013683 0.013446 0.005386 0.012202 0.017524 0.016232 0.006004
Nucleus 0.952078* 0.934173* 0.935639* 0.911693* 0.937913* 0.943281* 0.939996* 0.920079* 0.946485* 0.939558* 0.938955* 0.916670*
Peroxisomes 0.000372 0.001221 0.000914 0.000375 0.000485 0.001202 0.000337 0.000208 0.000416 0.001210 0.000475 0.000276
Vacuole 0.001319 0.001747 0.005850 0.005146 0.000575 0.002646 0.001310 0.002569 0.001025 0.002278 0.002394 0.003617
Vacuole Periphery 0.000164 0.000494 0.002164 0.001314 0.000348 0.000625 0.000158 0.001074 0.000237 0.000572 0.000637 0.001172

Localization Changes (T-score)

Change in localization of each mutant relative to its control (e.g. UBP2_WT: ubp2Δ vs wild type). Values highlighted in yellow (increase) or blue (decrease) denote significant changes (marked * in the plot).

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -9.95 -10.67 -3.71 -2.18 2.96 -1.26 -7.69 -4.64 -4.29 -1.64 -8.87 -13.24 -5.86 -4.16 0.16
Bud Neck -3.54 -4.60 -17.26 -12.43 -11.92 -2.29 -2.63 -22.59 -20.57 -16.39 -4.08 -3.89 -28.17 -23.49 -19.77
Bud Site -1.65 -2.77 -3.51 -2.15 -1.92 -2.90 -3.50 -6.07 -3.89 -4.54 -2.40 -2.37 -6.17 -4.23 -5.01
Cell Periphery 2.93 2.23 2.81 -0.56 1.23 -2.39 -2.98 -1.46 -0.57 -0.32 0.84 -0.35 -0.17 -0.63 0.04
Cytoplasm 9.04 13.69 10.78 1.01 -3.07 4.75 1.32 5.22 0.50 2.82 10.74 6.34 12.07 1.07 2.39
Cytoplasmic Foci -0.04 4.42 1.99 1.67 -2.42 -0.85 -0.15 1.98 2.79 1.52 -0.54 0.83 3.00 3.21 1.07
Eisosomes -9.14 -11.69 -18.25 -5.12 8.39 -2.13 -10.16 -7.24 -3.87 5.40 -7.87 -15.85 -17.76 -6.07 8.88
Endoplasmic Reticulum -11.47 -9.29 -15.83 -3.31 5.42 -6.30 -6.29 -13.24 -3.11 -5.21 -11.72 -11.06 -21.29 -4.43 0.52
Endosome -1.85 -0.50 -2.61 -0.65 -2.39 -2.02 0.94 -1.25 0.72 -2.20 -2.93 0.70 -2.62 0.28 -3.18
Golgi -2.24 -0.38 -2.47 -1.19 -2.44 -2.37 -0.07 -1.80 0.55 -1.71 -3.21 -0.28 -3.08 -0.42 -2.92
Lipid Particles -1.11 -0.62 1.48 2.01 3.89 -0.32 1.78 3.04 3.38 3.57 -1.22 1.10 3.08 3.70 4.58
Mitochondria -2.31 -0.77 -3.31 0.73 -3.28 -3.43 -0.33 -3.31 1.92 -1.20 -4.27 -0.52 -5.08 2.00 -1.46
Mitotic Spindle -2.00 -1.32 -5.12 -3.62 -2.23 -1.07 0.35 -3.73 -2.72 -3.37 -2.51 -0.78 -6.72 -4.43 -4.21
None 0.81 5.42 2.97 1.67 -1.85 4.54 -1.65 6.20 1.76 4.80 3.77 -1.78 6.66 2.42 4.44
Nuclear Periphery 3.51 -0.25 -1.65 -5.44 -0.36 -1.62 1.89 -7.15 -5.03 -8.68 1.32 1.66 -5.62 -6.95 -6.29
Nuclear Periphery Foci -2.59 -1.99 -1.32 1.14 0.49 2.25 2.71 2.12 -0.43 -2.18 0.52 2.08 1.09 0.77 -1.44
Nucleolus -9.77 -4.93 -3.64 7.66 3.83 5.03 5.19 10.24 5.30 3.24 -4.37 -1.22 5.80 9.15 4.56
Nucleus 3.80 3.44 7.89 3.32 0.67 -1.36 -0.83 3.57 4.83 3.69 1.94 1.98 8.75 5.82 3.83
Peroxisomes -2.54 -3.80 0.77 2.85 4.48 -2.30 1.58 2.93 3.89 1.69 -3.57 -0.43 2.69 4.80 3.39
Vacuole -0.70 -2.98 -4.51 -3.57 2.03 -2.79 -3.09 -6.73 -0.79 -1.29 -2.46 -3.63 -6.85 -3.15 1.05
Vacuole Periphery -2.27 -1.06 -3.26 -1.30 0.51 -1.53 1.46 -1.60 -0.26 -2.87 -2.98 -0.78 -3.57 -0.99 -0.85
Standard name
Human Ortholog
Description (SGD) Trimeric heat shock transcription factor; forms condensate with Mediator and RNA Pol II upon heat shock; activates multiple genes in response to diverse stresses and transient intracellular acidification; recognizes variable heat shock elements (HSEs) consisting of inverted NGAAN repeats; monitors translational status through RQC (Ribosomal Quality Control)-mediated translation-stress signal; human homolog HSF1 with linker region mutations can complement yeast hsf1 mutant
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Hsf1

Hsf1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hsf1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available