Standard name | |
---|---|
Human Ortholog | |
Description | Plasma membrane protein; involved in G-protein mediated pheromone signaling pathway; overproduction suppresses bem1 mutations; MDG1 has a paralog, CRP1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 3 | 3 | 31 | 63 | 153 | 195 | 222 | 259 | – | 307 | 251 | 338 | 18 | 27 | 94 | 14 | 9 | 10 | 41 | 9 | 30 | 14 | 19 | 18 |
Bud | 0 | 0 | 17 | 15 | 48 | 58 | 47 | 73 | – | 62 | 62 | 91 | 7 | 5 | 12 | 3 | 4 | 0 | 3 | 0 | 0 | 0 | 4 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 1 | 3 | 5 | 7 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 3 | 1 | 3 | – | 3 | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 65 | 9 | 76 | 92 | 118 | 170 | 211 | 212 | – | 203 | 204 | 275 | 110 | 117 | 138 | 114 | 97 | 95 | 89 | 27 | 114 | 50 | 107 | 79 |
Cytoplasm | 118 | 39 | 90 | 69 | 79 | 134 | 129 | 101 | – | 93 | 76 | 75 | 93 | 51 | 45 | 42 | 39 | 39 | 11 | 15 | 12 | 11 | 16 | 22 |
Endoplasmic Reticulum | 20 | 15 | 41 | 73 | 56 | 62 | 73 | 48 | – | 74 | 27 | 33 | 33 | 49 | 50 | 3 | 0 | 2 | 161 | 31 | 75 | 35 | 115 | 119 |
Endosome | 4 | 0 | 0 | 0 | 2 | 0 | 5 | 8 | – | 4 | 0 | 0 | 0 | 0 | 2 | 2 | 1 | 0 | 1 | 1 | 0 | 0 | 2 | 0 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | – | 13 | 1 | 1 | 0 | 0 | 2 | 4 | 0 | 2 | 1 | 1 | 0 | 0 | 0 | 0 |
Mitochondria | 10 | 2 | 7 | 19 | 57 | 165 | 224 | 283 | – | 247 | 298 | 450 | 0 | 0 | 1 | 4 | 4 | 5 | 36 | 2 | 5 | 4 | 15 | 7 |
Nucleus | 9 | 1 | 0 | 1 | 0 | 2 | 1 | 1 | – | 0 | 0 | 0 | 1 | 1 | 0 | 9 | 6 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 1 | 1 | 0 | 1 | 3 | 5 | – | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 1 | 0 | 0 |
Nucleolus | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | – | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1 | 0 | 0 | 2 | 33 | 11 | 31 | 59 | – | 69 | 46 | 85 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 2 | 7 | 2 | 7 | 9 | – | 10 | 4 | 7 | 2 | 3 | 7 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 2 | 0 | 0 | 0 | 1 | 1 | 5 | 6 | – | 4 | 2 | 0 | 1 | 0 | 1 | 20 | 3 | 4 | 8 | 4 | 2 | 5 | 22 | 36 |
Unique Cell Count | 198 | 57 | 205 | 243 | 331 | 497 | 603 | 658 | 646 | 546 | 764 | 203 | 201 | 254 | 190 | 137 | 142 | 370 | 98 | 249 | 131 | 319 | 293 | |
Labelled Cell Count | 235 | 69 | 264 | 337 | 556 | 804 | 960 | 1071 | 1090 | 973 | 1363 | 265 | 253 | 356 | 216 | 163 | 161 | 370 | 98 | 249 | 131 | 319 | 293 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 10.2 | 9.1 | 7.1 | 7.2 | 7.8 | 7.5 | 7.2 | 7.5 | – | 7.7 | 7.5 | 7.3 | 9.7 | 10.2 | 10.1 | 18.3 | 18.1 | 18.3 | 10.4 | 11.0 | 10.6 |
Std Deviation (1e-4) | 10.3 | 1.8 | 1.4 | 1.3 | 1.5 | 1.5 | 1.5 | 1.5 | – | 1.8 | 1.9 | 1.8 | 1.6 | 2.0 | 1.9 | 4.3 | 4.3 | 3.9 | 2.5 | 3.6 | 1.9 |
Intensity Change (Log2) | – | – | – | 0.04 | 0.14 | 0.1 | 0.03 | 0.09 | – | 0.12 | 0.09 | 0.05 | 0.45 | 0.53 | 0.52 | 1.38 | 1.36 | 1.37 | 0.56 | 0.64 | 0.59 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 2.8 | 7.4 | 6.2 | 5.8 | 6.4 | – | 8.3 | 7.8 | 7.6 | -1.9 | -0.5 | 5.2 | -2.4 | -2.4 | -2.3 |
Bud | -0.9 | 2.1 | 1.3 | -0.2 | 1.1 | – | 0.6 | 1.2 | 1.5 | -2.1 | -2.6 | -1.6 | -3.0 | -2.0 | -3.5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.2 | -0.3 | -0.7 | -0.5 | -1.3 | – | -1.5 | 0.1 | -0.3 | 3.5 | 4.3 | 3.7 | 4.6 | 6.1 | 5.5 |
Cytoplasm | -3.4 | -4.9 | -4.4 | -6.3 | -8.6 | – | -9.0 | -8.8 | -11.5 | 0.4 | -3.9 | -6.1 | -4.6 | -2.9 | -3.1 |
Endoplasmic Reticulum | 2.4 | -0.9 | -2.6 | -2.8 | -5.2 | – | -3.1 | -6.4 | -7.5 | -1.0 | 1.1 | -0.1 | -5.8 | -5.6 | -5.2 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2.0 | 4.8 | 8.3 | 9.2 | 10.5 | – | 9.5 | 12.7 | 14.1 | 0 | 0 | 0 | -0.8 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 4.7 | 0 | 3.3 | 4.4 | – | 4.9 | 4.3 | 5.0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 4.8 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.471 | 4.8043 | 4.1282 | 3.5943 | 2.5245 | 4.1072 | 9.1637 | 9.3308 | 8.4034 | 7.4189 | 7.4056 | 8.4752 | 6.3447 | 5.8804 | 5.1526 | 5.207 | 4.45 | 5.1953 |
Actin | 0.0229 | 0.0229 | 0.0138 | 0.0108 | 0.0385 | 0.0469 | 0.0389 | 0.0107 | 0.0157 | 0.0118 | 0.0428 | 0.0177 | 0.039 | 0.011 | 0.03 | 0.0638 | 0.004 | 0.0301 |
Bud | 0.0039 | 0.0004 | 0.0056 | 0.001 | 0.0074 | 0.0066 | 0.0004 | 0.0002 | 0.0003 | 0.0008 | 0.0005 | 0.0002 | 0.0005 | 0.0003 | 0.0007 | 0.0009 | 0.0004 | 0.0006 |
Bud Neck | 0.0033 | 0.0062 | 0.0154 | 0.0194 | 0.0108 | 0.0198 | 0.0038 | 0.0048 | 0.005 | 0.0082 | 0.0153 | 0.0077 | 0.0077 | 0.0044 | 0.0072 | 0.0177 | 0.0201 | 0.0094 |
Bud Periphery | 0.0061 | 0.0022 | 0.0069 | 0.0063 | 0.0239 | 0.0172 | 0.0034 | 0.0012 | 0.0039 | 0.0099 | 0.0045 | 0.006 | 0.0042 | 0.0026 | 0.0051 | 0.0106 | 0.0099 | 0.011 |
Bud Site | 0.0158 | 0.077 | 0.026 | 0.0227 | 0.0094 | 0.0142 | 0.0043 | 0.0546 | 0.0122 | 0.0076 | 0.0064 | 0.0009 | 0.0078 | 0.0424 | 0.0144 | 0.0069 | 0.0025 | 0.002 |
Cell Periphery | 0.4407 | 0.477 | 0.4145 | 0.3903 | 0.3639 | 0.4546 | 0.5315 | 0.6192 | 0.6522 | 0.6196 | 0.5933 | 0.6842 | 0.4703 | 0.5891 | 0.5955 | 0.537 | 0.6477 | 0.6588 |
Cytoplasm | 0.1903 | 0.2592 | 0.275 | 0.2865 | 0.349 | 0.2524 | 0.0649 | 0.127 | 0.0962 | 0.0588 | 0.1143 | 0.0612 | 0.1436 | 0.1989 | 0.1658 | 0.1446 | 0.1873 | 0.1197 |
Cytoplasmic Foci | 0.0252 | 0.0019 | 0.0264 | 0.0251 | 0.0057 | 0.0067 | 0.0055 | 0.0024 | 0.0013 | 0.0005 | 0.0035 | 0.001 | 0.0148 | 0.0013 | 0.0067 | 0.0019 | 0.0003 | 0.0008 |
Eisosomes | 0.0451 | 0.076 | 0.0401 | 0.016 | 0.0108 | 0.0587 | 0.2045 | 0.0991 | 0.1199 | 0.1285 | 0.0922 | 0.1322 | 0.2017 | 0.0891 | 0.0632 | 0.0762 | 0.0636 | 0.0699 |
Endoplasmic Reticulum | 0.0445 | 0.0552 | 0.0705 | 0.0813 | 0.0278 | 0.0623 | 0.0759 | 0.0688 | 0.0791 | 0.1425 | 0.0451 | 0.0736 | 0.0528 | 0.0452 | 0.0723 | 0.1043 | 0.0532 | 0.0699 |
Endosome | 0.0423 | 0.0004 | 0.012 | 0.0417 | 0.0186 | 0.0029 | 0.0071 | 0.0003 | 0.0003 | 0.0003 | 0.0065 | 0.0006 | 0.0064 | 0.0002 | 0.0079 | 0.0014 | 0.0001 | 0.002 |
Golgi | 0.0239 | 0.0052 | 0.0075 | 0.0071 | 0.0192 | 0.0126 | 0.0061 | 0.001 | 0.0033 | 0.0021 | 0.0155 | 0.0024 | 0.0048 | 0.0023 | 0.0058 | 0.0045 | 0.0007 | 0.0037 |
Lipid Particles | 0.0155 | 0.0029 | 0.0112 | 0.018 | 0.0078 | 0.0065 | 0.0071 | 0.0024 | 0.0023 | 0.0033 | 0.0089 | 0.0045 | 0.0091 | 0.0045 | 0.0054 | 0.0087 | 0.0021 | 0.0047 |
Mitochondria | 0.0294 | 0.0002 | 0.0201 | 0.0218 | 0.0304 | 0.0132 | 0.0087 | 0.0001 | 0.0002 | 0.0002 | 0.0067 | 0.0002 | 0.0058 | 0.0001 | 0.0039 | 0.0092 | 0 | 0.0025 |
None | 0.0277 | 0.0095 | 0.0244 | 0.0146 | 0.0159 | 0.0099 | 0.0123 | 0.0042 | 0.0042 | 0.0013 | 0.0118 | 0.0021 | 0.0135 | 0.0052 | 0.003 | 0.0033 | 0.0011 | 0.0071 |
Nuclear Periphery | 0.0181 | 0.0011 | 0.0025 | 0.0081 | 0.0419 | 0.0013 | 0.0117 | 0.0018 | 0.002 | 0.0022 | 0.0211 | 0.0017 | 0.0064 | 0.0013 | 0.0045 | 0.0025 | 0.001 | 0.0037 |
Nucleolus | 0.0031 | 0.0002 | 0.004 | 0.0103 | 0.0009 | 0.0006 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0008 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 |
Nucleus | 0.0093 | 0.0003 | 0.0032 | 0.0042 | 0.0037 | 0.004 | 0.003 | 0.0001 | 0.0001 | 0.0002 | 0.0054 | 0.0001 | 0.0021 | 0.0003 | 0.0011 | 0.0005 | 0.0003 | 0.0004 |
Peroxisomes | 0.0153 | 0.0001 | 0.0097 | 0.0056 | 0.0004 | 0.0036 | 0.003 | 0.0004 | 0.0001 | 0 | 0.0017 | 0.0012 | 0.0036 | 0.0001 | 0.003 | 0.0004 | 0 | 0.0002 |
Punctate Nuclear | 0.0059 | 0.0001 | 0.0075 | 0.0032 | 0.0014 | 0.0023 | 0.0042 | 0.0003 | 0.0001 | 0.0001 | 0.0016 | 0.0004 | 0.002 | 0.0002 | 0.002 | 0.0002 | 0 | 0.0006 |
Vacuole | 0.0089 | 0.0017 | 0.0032 | 0.0045 | 0.0082 | 0.0034 | 0.0027 | 0.0012 | 0.0015 | 0.0017 | 0.0016 | 0.0019 | 0.0026 | 0.0014 | 0.0019 | 0.005 | 0.0054 | 0.0024 |
Vacuole Periphery | 0.0028 | 0 | 0.0007 | 0.0014 | 0.0045 | 0.0004 | 0.0005 | 0 | 0 | 0.0001 | 0.0008 | 0 | 0.0006 | 0 | 0.0002 | 0.0003 | 0 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 9.5207 | 8.9589 | 17.9493 | 38.581 | 33.1341 | 16.0261 | 15.1727 | 20.1755 | 47.3914 | 30.7965 |
Translational Efficiency | 1.884 | 1.8314 | 1.721 | 2.3624 | 1.8342 | 1.6001 | 1.4889 | 1.79 | 1.5495 | 1.8314 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2025 | 2184 | 149 | 1024 | 2249 | 1588 | 3588 | 2381 | 4274 | 3772 | 3737 | 3405 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 840.45 | 977.77 | 1452.83 | 1547.68 | 867.99 | 941.52 | 1312.55 | 1207.94 | 854.94 | 962.51 | 1318.14 | 1310.11 |
Standard Deviation | 134.70 | 164.40 | 244.35 | 283.51 | 121.74 | 139.21 | 213.13 | 325.01 | 128.78 | 155.33 | 216.21 | 349.73 |
Intensity Change Log 2 | — | 0.218333 | 0.789632 | 0.880873 | — | 0.117313 | 0.596622 | 0.476798 | — | 0.167893 | 0.694798 | 0.689697 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.022057 | 0.053520 | 0.135919* | 0.145282* | 0.016939 | 0.027574 | 0.054774 | 0.152179* | 0.019364 | 0.042597 | 0.058009 | 0.150105* |
Bud Neck | 0.022671 | 0.014655 | 0.027526 | 0.016422 | 0.017487 | 0.016624 | 0.019199 | 0.024704 | 0.019943 | 0.015484 | 0.019531 | 0.022214 |
Bud Site | 0.021485 | 0.017882 | 0.019258 | 0.023572 | 0.013612 | 0.021218 | 0.015655 | 0.124529* | 0.017342 | 0.019287 | 0.015799 | 0.094168 |
Cell Periphery | 0.208018* | 0.212076* | 0.289224* | 0.422039* | 0.175341* | 0.174159* | 0.632870* | 0.443939* | 0.190823* | 0.196113* | 0.619168* | 0.437353* |
Cytoplasm | 0.320771* | 0.131701* | 0.001629 | 0.002063 | 0.359607* | 0.292181* | 0.015585 | 0.039718 | 0.341207* | 0.199262* | 0.015029 | 0.028394 |
Cytoplasmic Foci | 0.016491 | 0.012929 | 0.005158 | 0.001512 | 0.018629 | 0.025134 | 0.001526 | 0.003355 | 0.017616 | 0.018067 | 0.001671 | 0.002800 |
Eisosomes | 0.184659* | 0.163728* | 0.158733* | 0.290600* | 0.186105* | 0.125030* | 0.182973* | 0.147428* | 0.185420* | 0.147436* | 0.182007* | 0.190484* |
Endoplasmic Reticulum | 0.060836 | 0.121313* | 0.208154* | 0.044444 | 0.066371 | 0.165610* | 0.020991 | 0.014962 | 0.063749 | 0.139962* | 0.028454 | 0.023828 |
Endosome | 0.001138 | 0.003734 | 0.026977 | 0.003859 | 0.001046 | 0.003425 | 0.001291 | 0.001604 | 0.001090 | 0.003604 | 0.002315 | 0.002283 |
Golgi | 0.012797 | 0.025319 | 0.023722 | 0.001954 | 0.014139 | 0.023557 | 0.000566 | 0.001219 | 0.013503 | 0.024577 | 0.001490 | 0.001440 |
Lipid Particles | 0.022231 | 0.071583 | 0.091802 | 0.029298 | 0.025367 | 0.038718 | 0.012496 | 0.008413 | 0.023881 | 0.057747 | 0.015658 | 0.014694 |
Mitochondria | 0.003818 | 0.004600 | 0.000219 | 0.000987 | 0.003505 | 0.003593 | 0.001364 | 0.001566 | 0.003653 | 0.004176 | 0.001319 | 0.001392 |
Mitotic Spindle | 0.000436 | 0.000678 | 0.000805 | 0.002040 | 0.000546 | 0.001046 | 0.001408 | 0.000560 | 0.000494 | 0.000833 | 0.001384 | 0.001005 |
None | 0.003613 | 0.005440 | 0.000055 | 0.000253 | 0.005241 | 0.003126 | 0.000723 | 0.000645 | 0.004470 | 0.004466 | 0.000697 | 0.000527 |
Nuclear Periphery | 0.000419 | 0.000627 | 0.000445 | 0.000892 | 0.000498 | 0.000646 | 0.001092 | 0.000232 | 0.000461 | 0.000635 | 0.001066 | 0.000430 |
Nuclear Periphery Foci | 0.000850 | 0.000749 | 0.002364 | 0.000340 | 0.001049 | 0.002627 | 0.000199 | 0.000187 | 0.000955 | 0.001540 | 0.000285 | 0.000233 |
Nucleolus | 0.000116 | 0.000078 | 0.000003 | 0.000008 | 0.000111 | 0.000145 | 0.000030 | 0.000015 | 0.000113 | 0.000106 | 0.000029 | 0.000013 |
Nucleus | 0.028234 | 0.010012 | 0.000287 | 0.000503 | 0.043960 | 0.032321 | 0.002192 | 0.016920 | 0.036509 | 0.019404 | 0.002116 | 0.011983 |
Peroxisomes | 0.063901 | 0.145187* | 0.002041 | 0.006801 | 0.044404 | 0.032789 | 0.030514 | 0.011118 | 0.053642 | 0.097868 | 0.029379 | 0.009820 |
Vacuole | 0.005271 | 0.004007 | 0.005551 | 0.006449 | 0.005863 | 0.010054 | 0.004506 | 0.006660 | 0.005583 | 0.006553 | 0.004547 | 0.006596 |
Vacuole Periphery | 0.000190 | 0.000180 | 0.000127 | 0.000683 | 0.000179 | 0.000425 | 0.000044 | 0.000046 | 0.000184 | 0.000283 | 0.000047 | 0.000237 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -9.54 | -6.40 | -15.09 | -10.81 | -0.50 | -4.02 | -13.19 | -27.05* | -23.41* | -17.91* | -10.58 | -14.06 | -30.63* | -23.57 | -18.97 |
Bud Neck | 4.64 | -1.02 | 3.75 | -1.24 | 2.38 | 0.54 | -1.39 | -5.08 | -4.95 | -4.29 | 3.85 | 0.37 | -1.98 | -5.98 | -2.46 |
Bud Site | 1.71 | 0.50 | -0.73 | -2.20 | -0.93 | -3.56 | -1.38 | -23.77 | -21.29 | -23.65* | -1.34 | 1.12 | -22.02 | -21.54 | -22.76 |
Cell Periphery | -0.58 | -3.27 | -19.24 | -18.85 | -5.04 | 0.21 | -71.10* | -32.74* | -30.87* | 22.63* | -1.06 | -73.18 | -37.30 | -35.41* | 24.53* |
Cytoplasm | 29.10 | 57.66 | 57.65 | 37.70 | -0.93 | 8.72 | 66.40* | 57.02 | 40.43 | -10.59 | 27.97 | 84.95* | 76.25 | 45.28 | -7.97 |
Cytoplasmic Foci | 2.86 | 5.24 | 13.51 | 12.33 | 1.83 | -3.51 | 15.72 | 12.88 | 13.42 | -3.80 | -0.43 | 21.07 | 17.93 | 17.30 | -2.95 |
Eisosomes | 3.49 | 1.48 | -11.28 | -13.93 | -7.01 | 10.56 | 0.59 | 6.84 | -3.91 | 7.18 | 9.08 | 0.77 | -0.93 | -8.89 | -1.60 |
Endoplasmic Reticulum | -11.68 | -6.54 | 3.58 | 14.20 | 7.24 | -14.59 | 14.12 | 15.96 | 24.17 | 3.49 | -18.38 | 13.36 | 15.86 | 30.48 | 2.19 |
Endosome | -9.12 | -4.92 | -6.63 | -0.28 | 4.39 | -5.89 | -2.91 | -3.88 | 4.38 | -2.34 | -10.64 | -5.20 | -7.45 | 4.82 | 0.11 |
Golgi | -7.49 | -2.64 | 13.25 | 14.80 | 5.30 | -5.65 | 16.75 | 15.08 | 15.04 | -2.16 | -9.23 | 20.99 | 20.47 | 21.06 | 0.15 |
Lipid Particles | -16.41 | -4.99 | -2.29 | 10.13 | 4.37 | -5.38 | 7.82 | 10.37 | 12.95 | 2.93 | -16.45 | 5.76 | 6.32 | 19.50 | 0.56 |
Mitochondria | -1.38 | 7.11 | 4.88 | 9.23 | -2.66 | -0.14 | 5.50 | 4.84 | 3.58 | -1.27 | -1.26 | 7.22 | 6.74 | 9.09 | -0.49 |
Mitotic Spindle | -4.30 | -3.13 | -5.67 | -4.84 | -4.12 | -1.06 | -5.94 | -0.09 | 1.07 | 13.37 | -1.60 | -10.03 | -4.40 | -0.65 | 3.72 |
None | -2.44 | 8.71 | 8.21 | 8.23 | -5.75 | 2.78 | 8.87 | 8.88 | 4.26 | 0.44 | -0.01 | 11.06 | 11.58 | 8.82 | 1.20 |
Nuclear Periphery | -5.44 | -0.46 | -6.95 | -3.60 | -5.26 | -1.16 | -14.30 | 10.16 | 3.24 | 21.69 | -2.99 | -16.13 | 1.13 | 3.35 | 15.19 |
Nuclear Periphery Foci | 2.03 | -2.48 | 6.53 | 5.56 | 3.28 | -6.84 | 12.87 | 11.84 | 10.85 | 0.16 | -5.66 | 14.08 | 14.60 | 12.90 | 1.14 |
Nucleolus | 1.87 | 7.37 | 7.06 | 5.33 | -6.71 | -0.81 | 4.93 | 5.86 | 2.91 | 10.42 | 0.19 | 7.48 | 8.93 | 4.56 | 12.70 |
Nucleus | 13.24 | 21.94 | 21.76 | 18.04 | -3.12 | 5.44 | 27.20 | 12.38 | 7.07 | -10.57 | 13.78 | 33.76 | 16.57 | 5.50 | -10.04 |
Peroxisomes | -16.71 | 21.86 | 18.98 | 33.39* | -3.86 | 4.26 | 5.54 | 14.56 | 10.34 | 10.76 | -14.24 | 10.95 | 23.21 | 32.40 | 12.02 |
Vacuole | 3.64 | -0.33 | -1.67 | -3.50 | -0.92 | -6.46 | 4.35 | -2.03 | 3.43 | -4.24 | -2.94 | 3.94 | -2.77 | -0.72 | -4.84 |
Vacuole Periphery | 0.21 | 1.11 | -1.09 | -1.12 | -1.24 | -1.70 | 2.20 | 2.16 | 2.88 | -0.33 | -1.44 | 3.50 | -0.39 | 0.30 | -1.41 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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