Standard name | |
---|---|
Human Ortholog | |
Description | Protein that associates with mitochondrial ribosome; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 8 | 17 | 4 | 8 | 2 | 3 | – | 0 | 3 | 0 | 3 | 15 | 18 | 1 | – | 2 | 0 | 7 | 14 | 4 | 6 | 7 | 9 |
Bud | 5 | 15 | 2 | 7 | 6 | 9 | 6 | – | 14 | 9 | 9 | 12 | 4 | 6 | 3 | – | 3 | 0 | 0 | 2 | 1 | 0 | 2 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 2 |
Bud Site | 0 | 0 | 1 | 0 | 0 | 0 | 3 | – | 1 | 1 | 4 | 3 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 11 | 24 | 13 | 2 | 4 | 4 | 5 | – | 3 | 4 | 1 | 5 | 5 | 14 | 13 | – | 11 | 2 | 0 | 1 | 1 | 0 | 0 | 0 |
Cytoplasm | 4 | 9 | 14 | 9 | 13 | 10 | 16 | – | 14 | 9 | 6 | 14 | 24 | 53 | 62 | – | 10 | 4 | 0 | 0 | 0 | 1 | 4 | 5 |
Endoplasmic Reticulum | 6 | 12 | 9 | 5 | 8 | 10 | 9 | – | 1 | 1 | 8 | 7 | 7 | 11 | 10 | – | 7 | 2 | 0 | 2 | 1 | 0 | 0 | 5 |
Endosome | 14 | 45 | 23 | 2 | 25 | 15 | 26 | – | 14 | 7 | 0 | 6 | 22 | 34 | 74 | – | 19 | 4 | 42 | 49 | 32 | 11 | 15 | 19 |
Golgi | 2 | 43 | 45 | 7 | 11 | 2 | 3 | – | 4 | 0 | 0 | 2 | 23 | 17 | 13 | – | 16 | 10 | 21 | 50 | 12 | 12 | 16 | 20 |
Mitochondria | 375 | 399 | 96 | 128 | 132 | 221 | 320 | – | 278 | 306 | 292 | 369 | 87 | 79 | 51 | – | 18 | 3 | 312 | 429 | 258 | 158 | 235 | 296 |
Nucleus | 1 | 5 | 1 | 4 | 3 | 7 | 20 | – | 5 | 5 | 4 | 4 | 2 | 0 | 0 | – | 2 | 2 | 0 | 0 | 0 | 0 | 2 | 0 |
Nuclear Periphery | 8 | 7 | 1 | 0 | 3 | 8 | 8 | – | 4 | 3 | 5 | 6 | 0 | 1 | 1 | – | 1 | 0 | 0 | 5 | 2 | 1 | 1 | 3 |
Nucleolus | 7 | 8 | 0 | 0 | 0 | 4 | 3 | – | 1 | 2 | 5 | 5 | 2 | 0 | 1 | – | 1 | 1 | 0 | 3 | 1 | 0 | 0 | 0 |
Peroxisomes | 10 | 27 | 8 | 4 | 8 | 6 | 7 | – | 5 | 3 | 0 | 3 | 13 | 26 | 79 | – | 10 | 1 | 23 | 24 | 33 | 9 | 13 | 18 |
SpindlePole | 6 | 9 | 21 | 8 | 15 | 13 | 33 | – | 15 | 20 | 9 | 28 | 7 | 9 | 19 | – | 15 | 5 | 0 | 2 | 2 | 2 | 0 | 0 |
Vac/Vac Membrane | 46 | 66 | 42 | 5 | 17 | 17 | 32 | – | 22 | 24 | 13 | 19 | 18 | 24 | 31 | – | 28 | 12 | 9 | 20 | 12 | 7 | 14 | 15 |
Unique Cell Count | 401 | 513 | 220 | 151 | 204 | 275 | 411 | 313 | 341 | 306 | 402 | 178 | 235 | 283 | 112 | 39 | 422 | 614 | 369 | 217 | 320 | 405 | ||
Labelled Cell Count | 495 | 677 | 293 | 185 | 254 | 328 | 494 | 381 | 398 | 356 | 486 | 229 | 292 | 358 | 143 | 46 | 422 | 614 | 369 | 217 | 320 | 405 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.1 | 6.2 | 6.5 | 5.5 | 6.1 | 5.2 | 5.4 | – | 4.9 | 4.9 | 4.2 | 4.7 | 6.6 | 6.5 | 6.4 | – | 7.6 | 7.1 | 6.1 | 6.3 | 6.4 |
Std Deviation (1e-4) | 0.6 | 0.9 | 1.0 | 1.4 | 1.1 | 1.3 | 1.1 | – | 1.4 | 1.0 | 0.8 | 0.9 | 1.4 | 1.3 | 1.2 | – | 1.5 | 1.5 | 1.1 | 1.2 | 1.2 |
Intensity Change (Log2) | – | – | – | -0.24 | -0.09 | -0.31 | -0.26 | – | -0.39 | -0.41 | -0.63 | -0.45 | 0.02 | 0.0 | -0.02 | – | 0.22 | 0.14 | -0.08 | -0.03 | -0.01 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -2.1 | -1.7 | -4.0 | -4.8 | – | -5.0 | 0 | 0 | 0 | 0.3 | 0 | -4.4 | – | -2.2 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Cell Periphery | -2.2 | -2.1 | -2.7 | -3.4 | – | -3.3 | 0 | 0 | 0 | -1.5 | 0 | -0.7 | – | 1.3 | 0 |
Cytoplasm | -0.2 | 0 | -1.4 | -1.4 | – | -1.0 | -2.2 | -2.6 | -1.7 | 2.4 | 4.9 | 4.8 | – | 0.9 | 0 |
Endoplasmic Reticulum | -0.4 | -0.1 | -0.3 | -1.4 | – | 0 | 0 | 0 | 0 | -0.1 | 0.3 | -0.3 | – | 0.9 | 0 |
Endosome | -3.4 | 0.6 | -2.1 | -1.8 | – | -2.7 | 0 | 0 | 0 | 0.6 | 1.3 | 4.4 | – | 1.7 | 0 |
Golgi | -4.3 | -4.6 | -7.4 | -8.9 | – | -7.5 | 0 | 0 | 0 | -2.0 | -4.1 | -5.5 | – | -1.4 | 0.7 |
Mitochondria | 8.0 | 4.3 | 8.5 | 8.6 | – | 11.2 | 0 | 0 | 0 | 1.0 | -2.2 | -6.3 | – | -5.0 | -4.3 |
Nucleus | 0 | 0 | 0 | 2.9 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Peroxisomes | 0 | 0.2 | -1.0 | -1.5 | – | -1.5 | 0 | 0 | 0 | 1.6 | 3.0 | 7.1 | – | 2.0 | 0 |
SpindlePole | -1.5 | -0.8 | -2.1 | -0.6 | – | -2.2 | 0 | 0 | 0 | -2.2 | -2.5 | -1.2 | – | 1.1 | 0 |
Vacuole | -4.5 | -3.2 | -4.4 | -4.2 | – | -4.2 | -4.3 | -5.5 | -5.8 | -2.5 | -2.7 | -2.6 | – | 1.2 | 1.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.9837 | 1.08 | 0.8654 | 0.4851 | 1.0411 | 0.9395 | 1.8003 | 1.7609 | 1.754 | 1.674 | 2.3714 | 1.7754 | 2.1877 | 2.0972 | 1.8857 | 2.3674 | 1.6606 | 2.0548 |
Actin | 0.0414 | 0.0384 | 0.0192 | 0.0237 | 0.0028 | 0.0027 | 0.0144 | 0.0076 | 0.0068 | 0.0007 | 0.0108 | 0.0122 | 0.0029 | 0.0014 | 0.0021 | 0.002 | 0.0014 | 0.0048 |
Bud | 0.0009 | 0.0026 | 0.0013 | 0.0019 | 0.0003 | 0.0004 | 0.0005 | 0.0019 | 0.001 | 0.0002 | 0.001 | 0.0004 | 0.0021 | 0.0011 | 0.0003 | 0.0001 | 0.0003 | 0.0017 |
Bud Neck | 0.0035 | 0.0037 | 0.0034 | 0.0004 | 0.0003 | 0.0005 | 0.0008 | 0.001 | 0.0009 | 0.0001 | 0.001 | 0.0016 | 0.0004 | 0.0012 | 0.0007 | 0.0001 | 0.0002 | 0.0008 |
Bud Periphery | 0.0015 | 0.0035 | 0.0034 | 0.0045 | 0.0013 | 0.0012 | 0.0012 | 0.0033 | 0.0016 | 0.0007 | 0.003 | 0.0009 | 0.0013 | 0.0018 | 0.001 | 0.0005 | 0.0008 | 0.0014 |
Bud Site | 0.0125 | 0.0343 | 0.0103 | 0.0009 | 0.0004 | 0.0006 | 0.0027 | 0.0233 | 0.0073 | 0.0004 | 0.0035 | 0.0009 | 0.0019 | 0.0078 | 0.001 | 0.0002 | 0.0005 | 0.001 |
Cell Periphery | 0.0011 | 0.0026 | 0.0019 | 0.0006 | 0.0004 | 0.0004 | 0.0005 | 0.0018 | 0.0004 | 0.0001 | 0.0007 | 0.0009 | 0.0003 | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0009 |
Cytoplasm | 0.0088 | 0.0053 | 0.0123 | 0.0044 | 0.0004 | 0.0002 | 0.0048 | 0.008 | 0.0047 | 0.0008 | 0.0022 | 0.0031 | 0.0016 | 0.0027 | 0.0014 | 0.0003 | 0.0004 | 0.0022 |
Cytoplasmic Foci | 0.0415 | 0.0352 | 0.0343 | 0.0097 | 0.0131 | 0.0046 | 0.0412 | 0.036 | 0.0266 | 0.0037 | 0.037 | 0.0192 | 0.0148 | 0.0194 | 0.0147 | 0.0055 | 0.012 | 0.0204 |
Eisosomes | 0.0012 | 0.0008 | 0.001 | 0.0005 | 0.0002 | 0.0002 | 0.0005 | 0.0007 | 0.0003 | 0.0001 | 0.0006 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0004 | 0.0001 | 0.0002 |
Endoplasmic Reticulum | 0.0077 | 0.0042 | 0.0012 | 0.0027 | 0.0003 | 0.0004 | 0.0015 | 0.003 | 0.0005 | 0.0003 | 0.0041 | 0.0003 | 0.001 | 0.0005 | 0.0004 | 0.0001 | 0.0001 | 0.0002 |
Endosome | 0.0888 | 0.0669 | 0.0447 | 0.0718 | 0.0844 | 0.0283 | 0.0807 | 0.0497 | 0.052 | 0.0352 | 0.1126 | 0.0237 | 0.0539 | 0.0521 | 0.0326 | 0.0055 | 0.0256 | 0.0311 |
Golgi | 0.0549 | 0.0726 | 0.0556 | 0.0409 | 0.1027 | 0.0354 | 0.0714 | 0.0477 | 0.0419 | 0.0076 | 0.0698 | 0.0391 | 0.0493 | 0.0663 | 0.0349 | 0.0086 | 0.0797 | 0.0453 |
Lipid Particles | 0.0745 | 0.0346 | 0.0178 | 0.0508 | 0.0235 | 0.0165 | 0.0584 | 0.0215 | 0.0152 | 0.003 | 0.0315 | 0.0075 | 0.0246 | 0.0123 | 0.0128 | 0.005 | 0.0055 | 0.0121 |
Mitochondria | 0.5471 | 0.6259 | 0.7491 | 0.7693 | 0.7548 | 0.875 | 0.604 | 0.7023 | 0.7912 | 0.9284 | 0.6547 | 0.8476 | 0.7963 | 0.7804 | 0.8582 | 0.9183 | 0.8329 | 0.8254 |
None | 0.0163 | 0.002 | 0.0065 | 0.0006 | 0.0003 | 0.0001 | 0.0241 | 0.0019 | 0.0051 | 0.0003 | 0.0047 | 0.0014 | 0.0008 | 0.0038 | 0.0004 | 0.0014 | 0.0001 | 0.0017 |
Nuclear Periphery | 0.0074 | 0.0033 | 0.0005 | 0.0012 | 0.0001 | 0.0002 | 0.001 | 0.001 | 0.0003 | 0.0019 | 0.0064 | 0.0001 | 0.001 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0002 |
Nucleolus | 0.0011 | 0.0005 | 0.0008 | 0.0001 | 0.0002 | 0.0002 | 0.0008 | 0.0006 | 0.0007 | 0.0004 | 0.004 | 0.0002 | 0.0015 | 0.0021 | 0.0004 | 0.0001 | 0.0001 | 0.0033 |
Nucleus | 0.0014 | 0.0006 | 0.0009 | 0.0002 | 0.0002 | 0.0002 | 0.0007 | 0.0006 | 0.0007 | 0.001 | 0.0033 | 0.0002 | 0.0007 | 0.0008 | 0.0004 | 0.0001 | 0.0001 | 0.0005 |
Peroxisomes | 0.0591 | 0.0462 | 0.0261 | 0.0082 | 0.0096 | 0.0255 | 0.0816 | 0.0793 | 0.0338 | 0.0087 | 0.0353 | 0.0374 | 0.0379 | 0.0387 | 0.03 | 0.0504 | 0.0383 | 0.0412 |
Punctate Nuclear | 0.0034 | 0.0003 | 0.0008 | 0.0002 | 0.0001 | 0 | 0.0018 | 0.0005 | 0.0005 | 0.0007 | 0.0018 | 0.0003 | 0.0006 | 0.0006 | 0.0011 | 0 | 0 | 0.0009 |
Vacuole | 0.018 | 0.0094 | 0.0054 | 0.0038 | 0.0021 | 0.003 | 0.0045 | 0.0054 | 0.0062 | 0.0036 | 0.007 | 0.0016 | 0.0035 | 0.0032 | 0.0036 | 0.0005 | 0.0006 | 0.0025 |
Vacuole Periphery | 0.0079 | 0.0071 | 0.0036 | 0.0036 | 0.0026 | 0.0044 | 0.0028 | 0.0028 | 0.0023 | 0.0021 | 0.0051 | 0.0014 | 0.0035 | 0.0032 | 0.0036 | 0.0008 | 0.0011 | 0.0021 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 4.8044 | 7.158 | 6.0198 | 7.8413 | 6.5751 | 2.1341 | 9.2881 | 11.3979 | 8.0745 | 9.3656 |
Translational Efficiency | 1.2627 | 1.1697 | 1.2312 | 0.8354 | 0.6401 | 1.9905 | 0.7625 | 0.7156 | 0.7517 | 0.8262 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
94 | 1295 | 1820 | 1269 | 1647 | 1487 | 970 | 147 | 1741 | 2782 | 2790 | 1416 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 777.88 | 737.10 | 830.41 | 903.68 | 707.01 | 758.73 | 937.37 | 989.92 | 710.84 | 748.66 | 867.60 | 912.63 |
Standard Deviation | 117.67 | 107.88 | 106.12 | 131.12 | 89.65 | 106.34 | 123.38 | 132.12 | 92.77 | 107.60 | 123.42 | 133.83 |
Intensity Change Log 2 | — | -0.077687 | 0.094276 | 0.216264 | — | 0.101856 | 0.406888 | 0.485581 | — | 0.010590 | 0.251583 | 0.350776 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000626 | 0.001244 | 0.004634 | 0.046308 | 0.000986 | 0.001873 | 0.014169 | 0.027676 | 0.000966 | 0.001580 | 0.007949 | 0.044374 |
Bud Neck | 0.006649 | 0.004775 | 0.004375 | 0.003552 | 0.003662 | 0.001937 | 0.001719 | 0.003218 | 0.003823 | 0.003258 | 0.003451 | 0.003517 |
Bud Site | 0.004618 | 0.012654 | 0.025610 | 0.070908 | 0.007472 | 0.006516 | 0.021557 | 0.054781 | 0.007318 | 0.009373 | 0.024201 | 0.069234 |
Cell Periphery | 0.000284 | 0.000433 | 0.000242 | 0.001264 | 0.000348 | 0.000268 | 0.000522 | 0.000675 | 0.000344 | 0.000344 | 0.000339 | 0.001202 |
Cytoplasm | 0.002437 | 0.003642 | 0.023548 | 0.011188 | 0.002995 | 0.002149 | 0.004469 | 0.012827 | 0.002965 | 0.002844 | 0.016914 | 0.011358 |
Cytoplasmic Foci | 0.060454 | 0.041089 | 0.046305 | 0.039249 | 0.041197 | 0.047846 | 0.084087 | 0.068536 | 0.042237 | 0.044701 | 0.059440 | 0.042289 |
Eisosomes | 0.000077 | 0.000082 | 0.000077 | 0.000214 | 0.000078 | 0.000085 | 0.000142 | 0.000174 | 0.000078 | 0.000084 | 0.000100 | 0.000210 |
Endoplasmic Reticulum | 0.000435 | 0.000381 | 0.002012 | 0.001078 | 0.000249 | 0.000572 | 0.002476 | 0.001921 | 0.000259 | 0.000483 | 0.002174 | 0.001166 |
Endosome | 0.070431 | 0.081515 | 0.081373 | 0.047292 | 0.074826 | 0.084384 | 0.086030 | 0.116582 | 0.074589 | 0.083049 | 0.082992 | 0.054485 |
Golgi | 0.064451 | 0.114650* | 0.079957 | 0.097321 | 0.121162* | 0.144564* | 0.181986* | 0.185004* | 0.118100* | 0.130640* | 0.115429* | 0.106424 |
Lipid Particles | 0.021062 | 0.002280 | 0.001512 | 0.000361 | 0.002090 | 0.001914 | 0.001115 | 0.000296 | 0.003114 | 0.002084 | 0.001374 | 0.000354 |
Mitochondria | 0.591412* | 0.651550* | 0.563035* | 0.559573* | 0.687413* | 0.635216* | 0.482668* | 0.339783* | 0.682230* | 0.642820* | 0.535094* | 0.536756* |
Mitotic Spindle | 0.012844 | 0.012127 | 0.060117 | 0.036039 | 0.006520 | 0.010951 | 0.030190 | 0.048997 | 0.006861 | 0.011498 | 0.049712 | 0.037385 |
None | 0.000601 | 0.000268 | 0.000288 | 0.000227 | 0.000217 | 0.000216 | 0.000197 | 0.000281 | 0.000238 | 0.000241 | 0.000256 | 0.000232 |
Nuclear Periphery | 0.000163 | 0.000105 | 0.000600 | 0.000082 | 0.000061 | 0.000068 | 0.000199 | 0.000348 | 0.000066 | 0.000085 | 0.000461 | 0.000110 |
Nuclear Periphery Foci | 0.000369 | 0.000211 | 0.001095 | 0.000617 | 0.000124 | 0.000111 | 0.000406 | 0.000557 | 0.000137 | 0.000157 | 0.000855 | 0.000611 |
Nucleolus | 0.014761 | 0.000671 | 0.000685 | 0.000040 | 0.000143 | 0.000740 | 0.000043 | 0.000054 | 0.000933 | 0.000708 | 0.000462 | 0.000041 |
Nucleus | 0.004739 | 0.000797 | 0.004276 | 0.000252 | 0.000379 | 0.001037 | 0.000453 | 0.000156 | 0.000614 | 0.000925 | 0.002947 | 0.000242 |
Peroxisomes | 0.129510* | 0.044640 | 0.039976 | 0.068392 | 0.029307 | 0.036889 | 0.047234 | 0.042467 | 0.034717 | 0.040497 | 0.042499 | 0.065700 |
Vacuole | 0.006046 | 0.011548 | 0.038844 | 0.010305 | 0.008201 | 0.010499 | 0.018743 | 0.064182 | 0.008084 | 0.010987 | 0.031855 | 0.015898 |
Vacuole Periphery | 0.008031 | 0.015338 | 0.021439 | 0.005737 | 0.012572 | 0.012166 | 0.021598 | 0.031484 | 0.012326 | 0.013642 | 0.021495 | 0.008410 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.47 | -5.85 | -12.30 | -12.13 | -11.05 | -2.36 | -7.28 | -3.30 | -3.19 | -1.62 | -2.25 | -8.75 | -12.61 | -12.44 | -10.35 |
Bud Neck | 0.95 | 1.16 | 1.61 | 1.27 | 1.39 | 2.16 | 2.62 | 0.39 | -1.34 | -1.62 | 0.73 | 0.39 | 0.29 | -0.57 | -0.10 |
Bud Site | -4.57 | -9.64 | -15.39 | -13.32 | -9.82 | 0.72 | -6.17 | -5.07 | -5.18 | -3.47 | -1.67 | -9.91 | -15.60 | -15.24 | -10.88 |
Cell Periphery | -1.50 | 0.48 | -5.94 | -5.47 | -7.04 | 1.35 | -2.07 | -2.85 | -3.83 | -1.25 | -0.00 | 0.10 | -6.27 | -6.54 | -6.57 |
Cytoplasm | -1.00 | -8.35 | -7.26 | -7.03 | 5.54 | 1.76 | -1.92 | -2.50 | -2.72 | -2.11 | 0.24 | -8.60 | -9.56 | -9.67 | 3.97 |
Cytoplasmic Foci | 1.98 | 1.44 | 2.15 | 0.63 | 2.50 | -2.27 | -10.65 | -3.39 | -2.55 | 1.87 | -0.96 | -6.61 | 0.05 | 0.97 | 6.35 |
Eisosomes | -0.46 | 0.01 | -10.38 | -13.95 | -14.45 | -0.62 | -5.05 | -3.33 | -3.25 | -1.09 | -0.55 | -2.12 | -10.36 | -14.18 | -12.08 |
Endoplasmic Reticulum | 0.47 | -3.29 | -3.41 | -4.14 | 1.89 | -1.19 | -3.09 | -3.46 | -2.45 | 0.65 | -1.50 | -4.80 | -5.71 | -3.28 | 2.38 |
Endosome | -0.81 | -0.75 | 1.75 | 7.86 | 8.66 | -2.10 | -2.42 | -3.68 | -2.82 | -2.68 | -2.16 | -2.06 | 5.15 | 8.21 | 8.31 |
Golgi | -4.26 | -1.28 | -2.79 | 2.71 | -3.30 | -3.50 | -7.56 | -3.64 | -2.26 | -0.14 | -2.31 | 0.66 | 1.96 | 4.34 | 1.53 |
Lipid Particles | 2.25 | 2.34 | 2.48 | 4.98 | 3.60 | 0.26 | 1.67 | 3.23 | 4.51 | 4.69 | 1.37 | 2.33 | 3.91 | 6.40 | 4.55 |
Mitochondria | -1.43 | 0.81 | 0.74 | 6.60 | -0.19 | 4.24 | 14.27 | 12.00 | 10.12 | 4.73 | 3.75 | 14.14 | 11.42 | 9.01 | -0.44 |
Mitotic Spindle | 0.22 | -10.43 | -5.93 | -8.37 | 5.78 | -2.96 | -7.38 | -4.32 | -3.85 | -1.82 | -3.88 | -16.48 | -11.92 | -9.83 | 3.63 |
None | 2.25 | 2.08 | 2.59 | 1.67 | 3.35 | 0.06 | 0.63 | -1.03 | -0.93 | -1.93 | -0.02 | -0.56 | 0.25 | 0.33 | 1.96 |
Nuclear Periphery | 0.90 | -2.63 | 1.19 | 0.63 | 3.30 | -0.50 | -4.04 | -1.53 | -1.49 | -0.78 | -1.22 | -3.85 | -1.48 | -0.84 | 3.31 |
Nuclear Periphery Foci | 1.40 | -2.12 | -0.57 | -0.94 | 0.90 | 0.44 | -3.60 | -2.36 | -2.44 | -0.70 | -0.66 | -3.28 | -1.22 | -1.16 | 0.58 |
Nucleolus | 1.44 | 1.44 | 1.51 | 1.82 | 2.58 | -1.71 | 3.41 | 2.72 | 1.98 | -0.45 | 0.37 | 0.80 | 1.67 | 2.71 | 2.58 |
Nucleus | 1.61 | -0.11 | 1.82 | 1.96 | 5.39 | -1.48 | -0.40 | 1.78 | 2.08 | 1.46 | -1.01 | -4.71 | 1.70 | 2.43 | 5.44 |
Peroxisomes | 3.62 | 3.84 | 2.60 | -4.21 | -5.53 | -2.01 | -3.76 | -1.30 | -0.53 | 0.50 | -1.66 | -2.22 | -6.42 | -5.41 | -4.97 |
Vacuole | -3.14 | -13.21 | -2.58 | 0.30 | 11.89 | -1.90 | -6.01 | -4.57 | -4.38 | -3.64 | -3.12 | -15.10 | -4.62 | -3.07 | 7.07 |
Vacuole Periphery | -3.28 | -6.10 | 1.20 | 6.87 | 12.02 | 0.29 | -4.47 | -3.26 | -3.30 | -1.64 | -1.15 | -7.15 | 3.52 | 4.52 | 10.15 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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