Standard name
Human Ortholog
Description Protein that associates with mitochondrial ribosome; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL027C is not an essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.05 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0.05 0 0 0
Bud 0 0 0 0 0 0 0 0 0.05 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.08 0.05 0 0 0 0 0 0
Cytoplasm 0.08 0.11 0.11 0.13 0.08 0 0 0 0 0 0 0 0.58 0.6 0.64 0.12 0.11 0.12 0 0 0 0.05 0 0
Endoplasmic Reticulum 0 0.08 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0.07 0 0 0 0 0 0 0
Endosome 0.11 0 0.1 0.08 0.14 0 0 0 0 0 0 0 0.06 0.1 0.11 0.18 0.21 0.27 0.13 0.12 0.1 0.05 0.05 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.14 0.15 0.11 0 0 0 0 0 0
Mitochondria 0.78 0.91 0.72 0.78 0.78 0.94 0.91 0.9 0.93 0.97 0.96 0.95 0.09 0.08 0.08 0.16 0.13 0.08 0.65 0.65 0.67 0.7 0.72 0.69
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.05 0 0.13 0.16 0.1 0 0 0 0 0 0 0 0 0 0 0.14 0.1 0.14 0 0 0.08 0 0 0
SpindlePole 0.06 0 0.05 0.06 0 0 0 0.06 0 0 0 0 0.05 0 0.05 0.07 0.13 0.15 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0.11 0.06 0 0.1 0.06 0 0.05 0 0 0 0.05 0.1 0.1 0.06 0.21 0.15 0.21 0 0.06 0 0.07 0.06 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 4 3 0 0 0 0 0 0 0 0 0 13 5 7 3 5 1 2 2 13 2 11 14
Bud 6 1 6 8 11 14 22 15 23 25 27 25 1 3 2 5 8 1 1 1 0 2 3 4
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 1 3 1 0 2 0 2 0 1 1 3 3
Bud Site 0 0 0 1 1 2 8 8 3 9 13 9 0 0 1 1 0 2 – – – – – –
Cell Periphery 1 0 10 5 4 1 4 1 1 2 0 4 5 10 7 17 14 7 0 0 0 1 0 2
Cytoplasm 18 8 28 28 27 11 23 12 15 6 11 10 89 135 173 27 20 16 1 0 1 12 13 10
Endoplasmic Reticulum 4 6 8 1 0 4 2 0 1 2 0 3 9 8 9 9 12 5 5 0 2 2 1 2
Endosome 24 0 27 16 50 5 5 10 6 0 2 0 9 23 31 42 38 35 36 13 29 12 19 25
Golgi 0 0 1 0 1 0 0 0 0 0 0 0 0 2 1 32 27 14 7 0 11 1 4 9
Mitochondria 170 68 191 162 270 353 519 472 426 555 430 480 14 17 21 38 23 10 178 69 201 175 294 304
Nucleus 3 0 1 0 4 6 16 17 6 13 7 9 1 1 2 10 1 4 0 0 0 3 2 3
Nuclear Periphery 0 1 0 1 0 17 23 17 8 21 6 15 0 0 0 1 1 0 5 0 2 0 0 3
Nucleolus 6 1 0 0 2 4 17 14 15 11 9 7 0 0 0 2 1 0 1 0 1 0 2 1
Peroxisomes 12 0 34 33 35 3 7 12 11 11 2 4 2 0 5 32 17 18 7 2 24 2 10 13
SpindlePole 13 0 13 12 13 13 15 31 19 19 8 21 7 10 14 17 24 20 4 2 4 9 8 11
Vac/Vac Membrane 16 8 15 5 36 21 13 27 20 20 14 24 15 22 15 48 27 27 11 6 1 17 25 22
Unique Cell Count 219 75 265 208 348 377 571 527 459 575 449 503 153 225 270 234 178 131 274 107 303 252 410 439
Labelled Cell Count 273 97 337 272 454 454 674 636 554 695 529 611 166 239 289 284 220 160 274 107 303 252 410 439


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 4.3 4.6 4.2 4.9 4.0 4.0 3.9 3.9 3.7 3.4 3.5 5.6 5.6 5.5 6.8 6.7 6.3 5.3 5.8 6.0
Std Deviation (1e-4) 0.8 0.5 1.4 1.8 2.3 1.3 1.6 1.2 1.5 0.9 1.0 1.1 0.8 1.1 1.0 1.3 1.4 1.3 1.0 1.2 1.5
Intensity Change (Log2) – – – -0.14 0.08 -0.21 -0.21 -0.23 -0.26 -0.32 -0.45 -0.4 0.27 0.28 0.25 0.56 0.54 0.45 0.19 0.33 0.36


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 5.0 3.3 4.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.6392 2.9493 3.0099 3.0425 2.8609 3.2166 0.399 0.7013 0.4321 -0.136 0.6145 0.6872 0.2954 0.5406 0.0483 0.1241 -0.4523 -0.0055
Actin 0.013 0.009 0.0271 0.007 0.0159 0.0251 0.0581 0.0195 0.04 0.0587 0.0839 0.0194 0.0493 0.0141 0.0385 0.0108 0.0423 0.027
Bud 0.0022 0.004 0.0077 0.0023 0.006 0.0031 0.0017 0.0036 0.0061 0.0065 0.0121 0.0019 0.0088 0.0055 0.0093 0.0062 0.0047 0.0018
Bud Neck 0.0036 0.0025 0.0053 0.0058 0.0021 0.019 0.0035 0.0017 0.0038 0.0093 0.0013 0.0238 0.0041 0.0047 0.0057 0.0017 0.0021 0.0128
Bud Periphery 0.0049 0.0062 0.0086 0.0065 0.0053 0.0066 0.0033 0.0034 0.0072 0.0057 0.0163 0.0025 0.0075 0.0068 0.0065 0.0083 0.0152 0.0026
Bud Site 0.0125 0.0508 0.0228 0.005 0.0022 0.0035 0.0126 0.04 0.0296 0.0125 0.0059 0.0028 0.0086 0.0554 0.024 0.0096 0.0073 0.0019
Cell Periphery 0.0055 0.0027 0.0044 0.0014 0.001 0.0057 0.0106 0.0012 0.0024 0.0011 0.002 0.0006 0.0011 0.0012 0.0041 0.0007 0.0087 0.0005
Cytoplasm 0.0631 0.1374 0.1083 0.0714 0.1003 0.0924 0.0372 0.1445 0.1472 0.2145 0.096 0.1476 0.0484 0.1578 0.1147 0.1411 0.0589 0.1346
Cytoplasmic Foci 0.1124 0.1304 0.1118 0.0651 0.0939 0.1177 0.1424 0.1381 0.1111 0.1 0.0691 0.0963 0.107 0.1329 0.1535 0.0909 0.1236 0.1477
Eisosomes 0.0031 0.0014 0.0013 0.0005 0.0003 0.0006 0.0007 0.0002 0.0006 0.0003 0.0013 0.0002 0.0007 0.0027 0.0011 0.0002 0.0018 0.0003
Endoplasmic Reticulum 0.0103 0.0057 0.006 0.0079 0.0022 0.0045 0.0105 0.0088 0.0099 0.0039 0.0132 0.0048 0.0158 0.0056 0.0071 0.0068 0.0115 0.0073
Endosome 0.1735 0.1536 0.1325 0.0936 0.2017 0.149 0.2919 0.2582 0.2305 0.273 0.2355 0.2036 0.218 0.232 0.1854 0.1782 0.2419 0.243
Golgi 0.0897 0.0858 0.0692 0.0526 0.069 0.1104 0.109 0.1428 0.0958 0.0891 0.152 0.1721 0.101 0.1183 0.1076 0.0955 0.0953 0.1119
Lipid Particles 0.0418 0.0197 0.0182 0.0121 0.0019 0.0173 0.0346 0.0087 0.0088 0.006 0.0426 0.0158 0.0252 0.0212 0.0092 0.0047 0.0123 0.0114
Mitochondria 0.297 0.3068 0.3252 0.4706 0.3582 0.3325 0.1688 0.153 0.2405 0.1642 0.1979 0.2375 0.2494 0.115 0.2561 0.3306 0.2679 0.1961
None 0.0948 0.0401 0.0883 0.1321 0.0811 0.063 0.0339 0.0414 0.0148 0.0025 0.0188 0.0142 0.0644 0.0707 0.0283 0.0796 0.0756 0.0362
Nuclear Periphery 0.0107 0.0018 0.0015 0.0014 0.0004 0.0007 0.0115 0.0026 0.0024 0.0016 0.0022 0.0008 0.0123 0.0024 0.0035 0.0013 0.0019 0.002
Nucleolus 0.0022 0.0022 0.0009 0.0009 0.0003 0.0011 0.0014 0.0003 0.0005 0.0005 0.0012 0.0004 0.002 0.0025 0.0009 0.0003 0.0005 0.0083
Nucleus 0.0056 0.002 0.0017 0.0011 0.0006 0.0016 0.0036 0.0012 0.0017 0.0071 0.0013 0.0012 0.0187 0.0032 0.0034 0.0012 0.0011 0.0017
Peroxisomes 0.0357 0.0217 0.0439 0.0452 0.0389 0.0355 0.0254 0.0122 0.0243 0.0161 0.0336 0.0363 0.0241 0.0193 0.0246 0.0179 0.0086 0.0324
Punctate Nuclear 0.0021 0.0023 0.001 0.0004 0.0004 0.0008 0.0175 0.001 0.002 0.0009 0.0012 0.0005 0.0052 0.0015 0.0045 0.0014 0.0032 0.001
Vacuole 0.0114 0.0109 0.0107 0.0128 0.0132 0.0064 0.0156 0.0128 0.0144 0.0194 0.0074 0.012 0.019 0.0198 0.0084 0.0082 0.0093 0.0132
Vacuole Periphery 0.0052 0.003 0.0037 0.0043 0.005 0.0034 0.0061 0.0048 0.0065 0.007 0.0053 0.0057 0.0091 0.0072 0.0036 0.0047 0.0064 0.006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.5991 4.6859 6.9542 15.9724 14.7252 10.4778 16.3852 12.791 17.2484 16.8602
Translational Efficiency 0.4775 1.64 0.8593 0.4273 0.4013 0.8809 0.3422 0.6152 0.495 0.4262

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1372 913 773 1533 873 2111 147 630 2245 3024 920 2163

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 643.36 697.13 916.42 898.51 647.47 754.95 1025.64 1113.92 644.96 737.49 933.87 961.25
Standard Deviation 87.15 112.03 95.72 127.14 94.60 88.72 123.43 180.18 90.14 99.94 108.33 174.62
Intensity Change Log 2 — 0.115801 0.510383 0.481908 — 0.221568 0.663639 0.782760 — 0.169822 0.589289 0.640640

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000923 0.004122 0.010862 0.038147 0.002159 0.001593 0.017233 0.010820 0.001404 0.002356 0.011880 0.030188
Bud Neck 0.022331 0.036361 0.028503 0.009904 0.038299 0.019712 0.020723 0.009595 0.028540 0.024739 0.027260 0.009814
Bud Site 0.013547 0.041045 0.050530 0.138395* 0.037721 0.022235 0.083317 0.047634 0.022947 0.027914 0.055769 0.111960*
Cell Periphery 0.000823 0.000442 0.000299 0.000819 0.000559 0.000217 0.000450 0.001601 0.000721 0.000285 0.000323 0.001047
Cytoplasm 0.035758 0.036815 0.145942* 0.040196 0.080558 0.033769 0.074841 0.024223 0.053179 0.034689 0.134581* 0.035544
Cytoplasmic Foci 0.169741 0.142292 0.162376 0.109057 0.245072* 0.160013 0.131439 0.027390 0.199034* 0.154663 0.157433 0.085270
Eisosomes 0.000311 0.000193 0.000180 0.000234 0.000301 0.000184 0.000226 0.000168 0.000307 0.000187 0.000188 0.000215
Endoplasmic Reticulum 0.001448 0.000765 0.017408 0.004688 0.003655 0.000900 0.015446 0.000593 0.002306 0.000859 0.017095 0.003496
Endosome 0.072524 0.135815 0.047315 0.078897 0.085056 0.099210 0.072003 0.028053 0.077397 0.110262 0.051259 0.064088
Golgi 0.126130* 0.108617 0.017029 0.112988* 0.086126 0.123110* 0.021507 0.059342 0.110574* 0.118734* 0.017745 0.097363
Lipid Particles 0.021083 0.008770 0.014696 0.002932 0.026061 0.007945 0.011318 0.000353 0.023018 0.008194 0.014157 0.002181
Mitochondria 0.431555* 0.315503* 0.020901 0.234029* 0.241241* 0.404530* 0.017614 0.635282* 0.357549* 0.377651* 0.020376 0.350899*
Mitotic Spindle 0.003865 0.016804 0.105442* 0.064822 0.010963 0.016044 0.097487 0.023562 0.006625 0.016274 0.104171* 0.052804
None 0.001348 0.001782 0.001353 0.000210 0.001235 0.000333 0.001212 0.000305 0.001304 0.000771 0.001330 0.000238
Nuclear Periphery 0.000389 0.000159 0.005120 0.000452 0.000517 0.000249 0.001924 0.000323 0.000439 0.000222 0.004609 0.000415
Nuclear Periphery Foci 0.000767 0.000344 0.007594 0.000820 0.000691 0.000252 0.005682 0.000222 0.000737 0.000279 0.007289 0.000645
Nucleolus 0.001887 0.003505 0.005752 0.000489 0.002809 0.001058 0.010468 0.000114 0.002245 0.001797 0.006506 0.000380
Nucleus 0.009152 0.009314 0.138230* 0.002156 0.017812 0.005081 0.122725 0.000486 0.012520 0.006359 0.135752* 0.001670
Peroxisomes 0.054772 0.085412 0.055031 0.072198 0.070828 0.049868 0.156203* 0.041574 0.061016 0.060599 0.071197 0.063279
Vacuole 0.017519 0.034470 0.158653 0.069421 0.033776 0.037950 0.125679 0.054186 0.023841 0.036899 0.153384 0.064984
Vacuole Periphery 0.014127 0.017468 0.006783 0.019145 0.014562 0.015748 0.012503 0.034173 0.014296 0.016267 0.007697 0.023522

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.92 -6.11 -12.17 -10.49 -8.23 1.01 -4.10 -3.84 -4.13 1.22 -2.21 -6.97 -12.61 -12.06 -7.23
Bud Neck -3.62 -2.46 5.67 7.68 8.97 5.43 3.75 8.11 4.81 4.19 1.68 0.12 9.84 9.48 9.76
Bud Site -8.73 -11.00 -24.49 -16.86 -15.50 4.55 -4.76 -2.00 -5.94 3.55 -2.63 -9.64 -21.90 -20.79 -12.08
Cell Periphery 1.87 2.65 0.06 -2.57 -3.74 3.67 1.04 -1.73 -2.33 -1.94 3.41 3.14 -1.36 -3.79 -3.62
Cytoplasm -0.55 -16.10 -1.65 -0.81 15.63 8.50 0.01 9.84 3.02 4.63 5.88 -13.13 5.49 -0.48 16.64
Cytoplasmic Foci 4.03 2.62 11.00 5.79 6.49 11.73 9.99 33.06 31.90 8.52 9.24 8.41 25.28 18.10 10.83
Eisosomes 3.64 4.09 2.36 -2.24 -3.18 4.31 1.94 3.95 0.62 2.11 5.25 5.24 3.92 -1.73 -1.58
Endoplasmic Reticulum 2.97 -14.79 -4.58 -5.63 10.08 3.48 -5.90 3.88 1.70 7.70 4.22 -14.98 -2.16 -5.28 12.86
Endosome -9.55 7.10 -1.41 8.85 -9.28 -2.61 2.01 11.21 18.03 4.14 -8.60 8.33 4.00 13.07 -4.87
Golgi 2.61 22.19 2.38 -0.49 -21.52* -6.27 10.85 4.04 10.81 -6.71 -1.77 25.13 3.03 5.05 -22.26
Lipid Particles 5.91 3.14 9.96 4.52 8.89 6.52 4.56 9.71 9.21 6.81 9.13 4.97 13.61 7.67 10.83
Mitochondria 8.57 44.31* 17.21 6.56 -28.24* -13.56 20.86 -24.25 -15.50 -44.69* -2.17 46.83* 0.80 2.94 -42.76*
Mitotic Spindle -7.37 -13.71 -15.23 -11.31 4.27 -2.28 -5.25 -3.36 -2.09 4.33 -7.03 -14.39 -14.96 -11.49 6.42
None -0.95 0.23 5.17 4.05 4.30 4.46 0.01 4.63 0.88 2.66 2.73 0.14 6.84 4.45 4.78
Nuclear Periphery 2.20 -8.04 -0.51 -2.83 7.93 2.35 -4.54 1.35 -0.61 5.03 2.55 -8.42 0.15 -2.19 8.43
Nuclear Periphery Foci 1.08 -7.66 -0.20 -3.33 8.12 3.65 -5.14 2.97 0.25 5.48 1.96 -9.09 0.31 -3.55 9.50
Nucleolus -1.66 -4.85 3.49 3.75 7.61 2.06 -2.22 3.34 3.73 2.95 1.02 -4.83 4.70 4.57 7.51
Nucleus -0.34 -18.55 5.24 4.49 19.74 5.77 -6.16 8.30 6.58 7.12 4.56 -19.07 9.34 6.35 20.90
Peroxisomes -5.27 0.91 -3.67 2.19 -4.03 4.05 -3.64 4.29 1.48 5.21 0.07 -0.62 -0.53 -0.60 0.20
Vacuole -6.02 -24.42 -15.23 -8.98 14.14 -1.33 -8.26 -3.83 -3.25 6.52 -6.78 -24.96 -14.28 -9.69 16.10
Vacuole Periphery -2.17 8.27 -3.26 -0.88 -9.47 -0.86 1.14 -4.65 -4.47 -4.50 -2.06 7.58 -5.59 -4.40 -10.12
Standard name
Human Ortholog
Description Protein that associates with mitochondrial ribosome; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL027C is not an essential gene
Localization
Cell Percentages mitochondrion (78%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Mrx9

Mrx9


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mrx9-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available