Standard name | |
---|---|
Human Ortholog | |
Description | Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 12 | 10 | 11 | 23 | 12 | 26 | 56 | 27 | 52 | 21 | 47 | 16 | 18 | 14 | 8 | 10 | 5 | 1 | 4 | 4 | 3 | 11 | 11 |
Bud | 0 | 0 | 1 | 0 | 3 | 0 | 3 | 8 | 4 | 4 | 5 | 11 | 2 | 1 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 8 | 12 | 2 | 26 | 18 | 20 | 30 | 9 | 37 | 25 | 37 | 14 | 32 | 21 | 15 | 8 | 13 | 0 | 0 | 1 | 2 | 2 | 7 |
Cytoplasm | 7 | 26 | 18 | 24 | 40 | 31 | 54 | 42 | 42 | 37 | 38 | 52 | 15 | 51 | 27 | 31 | 66 | 31 | 1 | 7 | 2 | 4 | 13 | 4 |
Endoplasmic Reticulum | 125 | 190 | 313 | 177 | 258 | 388 | 334 | 586 | 336 | 358 | 231 | 245 | 198 | 317 | 238 | 4 | 13 | 0 | 143 | 221 | 336 | 265 | 445 | 417 |
Endosome | 0 | 0 | 1 | 0 | 3 | 2 | 6 | 5 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 2 | 3 | 0 | 0 | 1 | 1 | 0 | 1 | 2 |
Golgi | 6 | 0 | 10 | 22 | 37 | 9 | 10 | 19 | 2 | 7 | 1 | 4 | 5 | 5 | 1 | 2 | 12 | 8 | 1 | 1 | 2 | 2 | 4 | 4 |
Mitochondria | 4 | 10 | 18 | 29 | 37 | 37 | 81 | 132 | 73 | 139 | 103 | 164 | 1 | 0 | 0 | 18 | 17 | 10 | 2 | 4 | 4 | 3 | 9 | 9 |
Nucleus | 2 | 1 | 1 | 1 | 0 | 1 | 1 | 3 | 1 | 0 | 2 | 0 | 0 | 1 | 1 | 1 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 15 | 15 | 45 | 24 | 23 | 50 | 38 | 86 | 48 | 57 | 34 | 48 | 11 | 11 | 6 | 7 | 6 | 11 | 1 | 3 | 7 | 5 | 2 | 3 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 4 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 18 | 23 | 18 | 25 | 85 | 49 | 181 | 117 | 44 | 61 | 30 | 98 | 15 | 16 | 11 | 16 | 42 | 11 | 0 | 12 | 2 | 4 | 14 | 25 |
Unique Cell Count | 140 | 236 | 346 | 238 | 408 | 486 | 610 | 837 | 475 | 572 | 382 | 533 | 227 | 384 | 284 | 85 | 159 | 82 | 153 | 260 | 368 | 294 | 512 | 494 |
Labelled Cell Count | 179 | 285 | 447 | 315 | 536 | 598 | 754 | 1088 | 587 | 756 | 490 | 711 | 277 | 452 | 319 | 104 | 182 | 90 | 153 | 260 | 368 | 294 | 512 | 494 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 12.3 | 11.5 | 11.3 | 11.7 | 12.6 | 11.8 | 11.9 | 12.0 | 11.9 | 11.3 | 11.4 | 11.1 | 13.9 | 14.4 | 14.1 | 8.0 | 9.6 | 11.9 | 15.4 | 15.0 | 15.5 |
Std Deviation (1e-4) | 2.6 | 3.0 | 2.2 | 2.4 | 2.7 | 2.2 | 2.2 | 2.3 | 2.3 | 2.2 | 2.4 | 2.4 | 2.5 | 2.6 | 2.5 | 1.5 | 2.2 | 2.4 | 2.4 | 2.6 | 2.6 |
Intensity Change (Log2) | – | – | – | 0.04 | 0.15 | 0.06 | 0.08 | 0.08 | 0.07 | -0.01 | 0.01 | -0.02 | 0.29 | 0.35 | 0.31 | -0.51 | -0.24 | 0.07 | 0.44 | 0.41 | 0.45 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 1.1 | 1.8 | -0.4 | 1.1 | 2.6 | 1.9 | 3.6 | 1.7 | 3.5 | 2.3 | 1.3 | 1.3 | 0 | 1.8 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -2.0 | 1.8 | 0.2 | -0.2 | 0.1 | -1.4 | 2.0 | 1.9 | 2.2 | 1.5 | 2.8 | 2.2 | 4.8 | 0.8 | 0 |
Cytoplasm | 2.2 | 2.4 | 0.7 | 2.1 | -0.1 | 2.0 | 0.8 | 2.4 | 2.4 | 0.7 | 3.7 | 2.1 | 8.1 | 10.2 | 8.3 |
Endoplasmic Reticulum | -5.2 | -8.7 | -4.1 | -11.3 | -7.5 | -6.9 | -9.2 | -9.3 | -13.4 | -1.2 | -3.1 | -2.5 | -16.1 | -18.0 | -16.6 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 3.3 | 3.5 | -1.0 | -1.3 | -0.6 | -2.9 | -1.8 | -2.9 | -2.5 | -0.5 | -1.5 | 0 | 0 | 2.4 | 0 |
Mitochondria | 3.0 | 2.0 | 1.4 | 3.9 | 5.0 | 4.6 | 7.4 | 7.9 | 9.1 | -3.1 | -4.5 | -3.9 | 4.8 | 2.3 | 2.3 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | -1.1 | -3.5 | -1.2 | -3.6 | -1.4 | -1.3 | -1.4 | -1.8 | -1.9 | -3.2 | -5.1 | -5.0 | -1.2 | -3.2 | 0.1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 2.4 | 6.2 | 2.5 | 9.0 | 4.3 | 2.2 | 2.9 | 1.4 | 5.6 | 0.7 | -0.7 | -0.8 | 4.2 | 6.8 | 2.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 6.9991 | 6.0085 | 6.5933 | 5.1328 | 6.2661 | 6.3397 | 5.2462 | 6.3767 | 5.376 | 5.2017 | 4.5525 | 5.4928 | 5.268 | 5.9007 | 4.9887 | 4.5925 | 4.1866 | 5.154 |
Actin | 0.0083 | 0.0057 | 0.0043 | 0.003 | 0.0056 | 0.0043 | 0.0128 | 0.0001 | 0.0053 | 0.001 | 0.0079 | 0.0013 | 0.0005 | 0.0257 | 0.0004 | 0.0095 | 0.0005 | 0.0018 |
Bud | 0.0002 | 0.0014 | 0.0029 | 0.0002 | 0.0099 | 0.0002 | 0.0003 | 0 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0.0001 |
Bud Neck | 0.0003 | 0.0004 | 0.0008 | 0.0004 | 0.0004 | 0.0008 | 0.0013 | 0.0001 | 0.0037 | 0.0003 | 0.0005 | 0.0006 | 0.0001 | 0.0006 | 0.0001 | 0.0003 | 0.0004 | 0.0012 |
Bud Periphery | 0.0007 | 0.0033 | 0.0049 | 0.0002 | 0.0084 | 0.0004 | 0.0003 | 0 | 0.0023 | 0.0005 | 0.0003 | 0.0002 | 0 | 0.0009 | 0 | 0.0004 | 0.0001 | 0.0004 |
Bud Site | 0.0009 | 0.01 | 0.0054 | 0.0002 | 0.0006 | 0.0001 | 0.0025 | 0.0001 | 0.001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0107 | 0.0001 | 0.0001 | 0 | 0.0003 |
Cell Periphery | 0.0007 | 0.0021 | 0.0013 | 0.0003 | 0.0005 | 0.0004 | 0.0007 | 0.0009 | 0.0029 | 0.0015 | 0.0009 | 0.0009 | 0.0007 | 0.0028 | 0.0005 | 0.0006 | 0.0004 | 0.001 |
Cytoplasm | 0.0446 | 0.1155 | 0.0622 | 0.054 | 0.026 | 0.0635 | 0.0433 | 0.0787 | 0.039 | 0.0737 | 0.0541 | 0.0651 | 0.0187 | 0.1098 | 0.0443 | 0.0564 | 0.0305 | 0.0827 |
Cytoplasmic Foci | 0.0077 | 0.007 | 0.0059 | 0.0101 | 0.0167 | 0.0035 | 0.004 | 0.0014 | 0.0025 | 0.0013 | 0.0026 | 0.0032 | 0.0125 | 0.0068 | 0.0028 | 0.0063 | 0.0012 | 0.0024 |
Eisosomes | 0.0004 | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0005 | 0.0001 | 0.0003 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 |
Endoplasmic Reticulum | 0.7993 | 0.6742 | 0.7967 | 0.7883 | 0.8011 | 0.7988 | 0.8147 | 0.8447 | 0.8412 | 0.8359 | 0.8451 | 0.8133 | 0.8574 | 0.7811 | 0.8718 | 0.8479 | 0.8865 | 0.8397 |
Endosome | 0.0187 | 0.0248 | 0.021 | 0.0275 | 0.0222 | 0.0157 | 0.0176 | 0.0022 | 0.0095 | 0.0054 | 0.0124 | 0.0173 | 0.014 | 0.0104 | 0.0081 | 0.0226 | 0.0058 | 0.0053 |
Golgi | 0.0032 | 0.003 | 0.0035 | 0.0048 | 0.0035 | 0.0023 | 0.0055 | 0.0003 | 0.0027 | 0.0009 | 0.0046 | 0.0012 | 0.0013 | 0.004 | 0.0012 | 0.004 | 0.0004 | 0.0004 |
Lipid Particles | 0.0085 | 0.0156 | 0.0086 | 0.035 | 0.0083 | 0.0027 | 0.0039 | 0.0016 | 0.0048 | 0.0011 | 0.0066 | 0.0046 | 0.0243 | 0.0075 | 0.0047 | 0.0117 | 0.0012 | 0.0034 |
Mitochondria | 0.0023 | 0.0027 | 0.0019 | 0.0008 | 0.0068 | 0.0017 | 0.0007 | 0 | 0.0105 | 0.0507 | 0.0006 | 0.0004 | 0.0003 | 0.0008 | 0.0003 | 0.0005 | 0.0001 | 0.0002 |
None | 0.0249 | 0.0475 | 0.024 | 0.0059 | 0.0074 | 0.0295 | 0.0426 | 0.0618 | 0.0453 | 0.0037 | 0.041 | 0.046 | 0.0204 | 0.0011 | 0.0496 | 0.022 | 0.0501 | 0.037 |
Nuclear Periphery | 0.0593 | 0.0566 | 0.0313 | 0.0513 | 0.0279 | 0.0572 | 0.0377 | 0.0052 | 0.0207 | 0.0146 | 0.0172 | 0.0355 | 0.041 | 0.0237 | 0.0121 | 0.0125 | 0.0167 | 0.0174 |
Nucleolus | 0.0002 | 0.0006 | 0.0004 | 0.0002 | 0.0006 | 0.0002 | 0.0003 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0003 | 0 | 0 | 0 | 0 |
Nucleus | 0.0065 | 0.0099 | 0.0077 | 0.0067 | 0.005 | 0.0102 | 0.0047 | 0.0004 | 0.0018 | 0.0021 | 0.0018 | 0.0034 | 0.0014 | 0.0043 | 0.0008 | 0.0008 | 0.0013 | 0.0025 |
Peroxisomes | 0.0021 | 0.0003 | 0.0027 | 0.0008 | 0.0142 | 0.0004 | 0.0006 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0 | 0 |
Punctate Nuclear | 0.0027 | 0.0023 | 0.0071 | 0.0042 | 0.0024 | 0.0014 | 0.0015 | 0.0002 | 0.0006 | 0.0002 | 0.0006 | 0.0006 | 0.0009 | 0.0009 | 0.0005 | 0.0007 | 0.0004 | 0.0003 |
Vacuole | 0.0066 | 0.0147 | 0.0057 | 0.0047 | 0.0263 | 0.0048 | 0.0038 | 0.002 | 0.0041 | 0.0053 | 0.0025 | 0.0052 | 0.0042 | 0.0069 | 0.0021 | 0.0026 | 0.0036 | 0.0033 |
Vacuole Periphery | 0.0019 | 0.0024 | 0.0015 | 0.0015 | 0.0061 | 0.0018 | 0.0011 | 0.0002 | 0.0009 | 0.0011 | 0.0006 | 0.0011 | 0.0019 | 0.0009 | 0.0005 | 0.0008 | 0.0008 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 57.0235 | 46.3877 | 65.439 | 95.4682 | 86.1325 | 43.3139 | 51.6701 | 55.3112 | 74.4498 | 98.5287 |
Translational Efficiency | 0.725 | 0.8168 | 0.7347 | 0.5797 | 0.7199 | 0.8332 | 0.7573 | 0.5752 | 0.6512 | 0.5989 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
109 | 1008 | 423 | 1445 | 1317 | 1405 | 1581 | 1479 | 1426 | 2413 | 2004 | 2924 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1291.57 | 1160.44 | 1393.80 | 1327.63 | 1066.72 | 1063.83 | 1443.03 | 1359.93 | 1083.91 | 1104.19 | 1432.64 | 1343.97 |
Standard Deviation | 185.46 | 243.40 | 369.22 | 347.70 | 237.23 | 219.29 | 279.01 | 316.86 | 241.19 | 234.56 | 300.98 | 332.85 |
Intensity Change Log 2 | — | -0.154454 | 0.109898 | 0.039727 | — | -0.003914 | 0.435920 | 0.350351 | — | -0.084409 | 0.266539 | 0.188556 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.003099 | 0.000992 | 0.002417 | 0.003464 | 0.001930 | 0.002286 | 0.002140 | 0.003176 | 0.002019 | 0.001745 | 0.002198 | 0.003318 |
Bud Neck | 0.006882 | 0.000410 | 0.004137 | 0.005670 | 0.000276 | 0.000774 | 0.001398 | 0.005020 | 0.000781 | 0.000622 | 0.001976 | 0.005341 |
Bud Site | 0.023245 | 0.004320 | 0.008795 | 0.025538 | 0.007381 | 0.005037 | 0.007155 | 0.023676 | 0.008593 | 0.004737 | 0.007502 | 0.024596 |
Cell Periphery | 0.002856 | 0.001428 | 0.002144 | 0.005235 | 0.001205 | 0.002266 | 0.002471 | 0.004756 | 0.001331 | 0.001916 | 0.002402 | 0.004993 |
Cytoplasm | 0.018744 | 0.022415 | 0.020462 | 0.070099 | 0.011037 | 0.025739 | 0.021316 | 0.044143 | 0.011626 | 0.024350 | 0.021136 | 0.056970 |
Cytoplasmic Foci | 0.026500 | 0.008163 | 0.005232 | 0.019344 | 0.011281 | 0.007673 | 0.004079 | 0.006210 | 0.012444 | 0.007878 | 0.004322 | 0.012701 |
Eisosomes | 0.000332 | 0.000047 | 0.000046 | 0.000203 | 0.000059 | 0.000183 | 0.000104 | 0.000152 | 0.000080 | 0.000126 | 0.000092 | 0.000177 |
Endoplasmic Reticulum | 0.650143* | 0.909536* | 0.806687* | 0.676870* | 0.903941* | 0.911399* | 0.886364* | 0.810647* | 0.884541* | 0.910621* | 0.869546* | 0.744536* |
Endosome | 0.026199 | 0.011348 | 0.028380 | 0.021885 | 0.017050 | 0.007427 | 0.012237 | 0.015536 | 0.017749 | 0.009065 | 0.015644 | 0.018674 |
Golgi | 0.024197 | 0.003877 | 0.012363 | 0.007282 | 0.007570 | 0.003645 | 0.003195 | 0.005427 | 0.008841 | 0.003742 | 0.005130 | 0.006344 |
Lipid Particles | 0.022852 | 0.008735 | 0.006117 | 0.028919 | 0.006845 | 0.007801 | 0.010126 | 0.012453 | 0.008069 | 0.008191 | 0.009280 | 0.020590 |
Mitochondria | 0.002911 | 0.001597 | 0.000944 | 0.002558 | 0.002784 | 0.002586 | 0.001651 | 0.002190 | 0.002794 | 0.002173 | 0.001502 | 0.002372 |
Mitotic Spindle | 0.018020 | 0.000187 | 0.000362 | 0.011205 | 0.000195 | 0.000150 | 0.001137 | 0.004244 | 0.001557 | 0.000165 | 0.000974 | 0.007684 |
None | 0.000041 | 0.004316 | 0.000882 | 0.004493 | 0.004425 | 0.005325 | 0.001636 | 0.001890 | 0.004089 | 0.004903 | 0.001477 | 0.003177 |
Nuclear Periphery | 0.004386 | 0.001786 | 0.011960 | 0.005529 | 0.002313 | 0.001302 | 0.005555 | 0.002404 | 0.002471 | 0.001504 | 0.006907 | 0.003949 |
Nuclear Periphery Foci | 0.064968 | 0.010992 | 0.059900 | 0.046750 | 0.012890 | 0.006952 | 0.017588 | 0.023598 | 0.016871 | 0.008640 | 0.026519 | 0.035039 |
Nucleolus | 0.000086 | 0.000064 | 0.000234 | 0.001251 | 0.000058 | 0.000228 | 0.000046 | 0.000076 | 0.000060 | 0.000159 | 0.000086 | 0.000657 |
Nucleus | 0.001283 | 0.002066 | 0.005345 | 0.018148 | 0.001433 | 0.003085 | 0.005246 | 0.008365 | 0.001422 | 0.002659 | 0.005267 | 0.013200 |
Peroxisomes | 0.001461 | 0.000350 | 0.000125 | 0.000717 | 0.000576 | 0.001376 | 0.000164 | 0.000292 | 0.000644 | 0.000948 | 0.000156 | 0.000502 |
Vacuole | 0.092823 | 0.006328 | 0.020178 | 0.042836 | 0.004783 | 0.003301 | 0.015337 | 0.023845 | 0.011513 | 0.004566 | 0.016359 | 0.033230 |
Vacuole Periphery | 0.008971 | 0.001045 | 0.003290 | 0.002003 | 0.001970 | 0.001464 | 0.001054 | 0.001899 | 0.002505 | 0.001289 | 0.001526 | 0.001950 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.75 | 0.49 | -0.55 | -4.67 | -1.70 | -0.43 | -0.45 | -2.29 | -1.61 | -1.59 | 0.42 | -0.45 | -2.86 | -3.63 | -2.18 |
Bud Neck | 3.24 | 0.94 | 0.37 | -8.78 | -0.98 | -2.35 | -6.22 | -6.51 | -5.73 | -4.81 | 0.86 | -3.72 | -9.30 | -9.85 | -5.91 |
Bud Site | 2.49 | 1.85 | -0.56 | -9.14 | -6.16 | 1.36 | -0.25 | -7.29 | -8.62 | -7.24 | 2.45 | 0.31 | -8.88 | -12.55 | -9.93 |
Cell Periphery | 1.69 | 0.92 | -1.72 | -5.28 | -3.96 | -2.15 | -2.94 | -6.01 | -3.37 | -3.12 | -1.65 | -2.89 | -7.76 | -5.81 | -4.52 |
Cytoplasm | -0.08 | 0.12 | -6.63 | -10.61 | -9.81 | -4.92 | -5.21 | -10.99 | -6.33 | -7.07 | -5.22 | -5.00 | -16.92 | -12.11 | -12.51 |
Cytoplasmic Foci | 2.06 | 2.43 | 0.74 | -4.97 | -7.25 | 1.76 | 3.91 | 2.47 | 0.68 | -2.29 | 2.35 | 4.53 | -0.50 | -3.98 | -7.66 |
Eisosomes | 2.45 | 2.44 | 1.03 | -3.40 | -3.31 | -3.25 | -1.68 | -4.40 | 0.60 | -1.54 | -1.84 | -0.58 | -3.61 | -1.67 | -2.67 |
Endoplasmic Reticulum | -7.00 | -4.15 | -0.25 | 21.15 | 9.19 | -0.82 | 3.28 | 11.88 | 12.51 | 9.05 | -3.40 | 2.60 | 18.07 | 23.98 | 16.48 |
Endosome | 3.51 | -0.47 | 1.02 | -6.87 | 2.32 | 6.83 | 2.93 | 0.90 | -6.61 | -2.20 | 6.64 | 1.19 | -0.67 | -9.90 | -2.27 |
Golgi | 2.06 | 1.11 | 1.73 | -2.37 | 1.90 | 3.60 | 3.82 | 1.74 | -1.51 | -1.84 | 4.12 | 2.65 | 2.02 | -2.83 | -0.90 |
Lipid Particles | 3.02 | 3.62 | -1.10 | -7.78 | -9.33 | -0.68 | -2.35 | -3.57 | -3.00 | -0.97 | -0.08 | -1.16 | -7.76 | -8.28 | -6.48 |
Mitochondria | 0.66 | 0.97 | 0.12 | -2.21 | -3.34 | 0.21 | 1.10 | 0.33 | 0.14 | -1.20 | 0.77 | 1.42 | 0.28 | -1.01 | -2.03 |
Mitotic Spindle | 1.72 | 1.70 | 0.54 | -6.69 | -6.58 | 0.54 | -2.68 | -5.19 | -5.24 | -3.90 | 1.73 | 0.58 | -5.58 | -8.40 | -7.23 |
None | -3.31 | -3.15 | -3.92 | -0.04 | -3.07 | -0.52 | 1.96 | 1.88 | 2.52 | -0.41 | -0.56 | 2.05 | 0.72 | 1.62 | -2.12 |
Nuclear Periphery | 1.58 | -3.40 | -0.70 | -4.89 | 3.71 | 1.32 | -3.09 | -0.21 | -2.17 | 3.42 | 1.41 | -4.64 | -2.10 | -5.31 | 3.57 |
Nuclear Periphery Foci | 3.97 | 0.30 | 1.28 | -10.07 | 1.85 | 3.04 | -2.18 | -3.75 | -6.61 | -1.94 | 3.98 | -3.76 | -6.66 | -12.20 | -2.96 |
Nucleolus | 0.71 | -1.30 | -2.75 | -2.89 | -2.32 | -2.59 | 0.33 | -1.38 | 2.18 | -1.68 | -2.43 | -1.22 | -2.95 | -2.38 | -2.77 |
Nucleus | -0.44 | -2.19 | -10.78 | -10.78 | -6.86 | -1.95 | -3.91 | -8.16 | -7.03 | -3.66 | -1.73 | -4.31 | -12.80 | -12.69 | -7.84 |
Peroxisomes | 1.41 | 1.71 | 0.95 | -3.06 | -5.99 | -2.98 | 3.44 | 2.32 | 4.23 | -1.50 | -1.65 | 3.95 | 1.06 | 2.80 | -4.95 |
Vacuole | 4.76 | 4.05 | 2.36 | -13.46 | -7.88 | 1.69 | -6.66 | -10.51 | -11.11 | -4.97 | 4.10 | -2.54 | -11.29 | -17.58 | -9.60 |
Vacuole Periphery | 1.40 | 0.97 | 1.24 | -1.78 | 1.71 | 1.05 | 2.35 | 0.26 | -0.64 | -1.50 | 2.17 | 1.69 | 1.09 | -1.49 | -0.83 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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