Standard name
Human Ortholog
Description Mitochondrial translational activator specific for the COX3 mRNA; acts together with Pet54p and Pet122p; located in the mitochondrial inner membrane
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 – 0 0 0 0 0 0 0
Bud 0 0 0 0 0.05 0 0.06 0.07 – 0.07 0.06 0 0 0 0 0.05 – 0.05 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Cytoplasm 0.16 0.24 0.12 0.12 0.1 0.09 0.06 0.06 – 0 0.07 0 0.74 0.83 0.89 0.39 – 0.33 0 0 0 0 0 0.06
Endoplasmic Reticulum 0.05 0 0 0 0 0 0 0 – 0 0 0 0.05 0.09 0 0.13 – 0.08 0 0 0 0 0 0
Endosome 0.12 0 0.11 0.11 0.24 0 0 0 – 0 0 0 0.06 0 0 0.06 – 0.15 0.14 0.1 0.12 0.08 0.08 0.07
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Mitochondria 0.55 0.74 0.68 0.77 0.65 0.91 0.9 0.88 – 0.95 0.95 0.95 0 0 0 0.15 – 0.14 0.64 0.64 0.66 0.63 0.64 0.62
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Peroxisomes 0 0 0.11 0.09 0.12 0 0 0 – 0 0 0 0 0 0 0 – 0.07 0 0 0.06 0 0 0
SpindlePole 0.07 0 0.08 0.07 0.06 0 0.08 0.08 – 0.05 0 0.05 0 0 0 0.18 – 0.16 0 0 0 0 0 0
Vac/Vac Membrane 0.16 0.06 0.07 0 0.09 0 0 0 – 0 0 0 0.06 0 0 0.14 – 0.11 0 0.08 0 0.05 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 2 0 0 0 0 0 0 – 0 0 0 8 5 3 1 – 1 4 9 11 10 18 20
Bud 2 10 8 11 17 10 28 32 – 26 21 16 1 0 0 4 – 5 0 2 3 4 8 14
Bud Neck 0 0 2 0 0 0 0 1 – 0 0 1 5 1 3 2 – 1 1 3 3 5 13 10
Bud Site 0 0 3 0 1 1 6 8 – 14 10 9 0 0 0 0 – 1 – – – – – –
Cell Periphery 0 2 12 3 3 2 2 0 – 2 3 1 5 6 0 1 – 2 0 0 1 1 2 6
Cytoplasm 22 60 40 32 32 29 31 30 – 17 26 16 131 203 254 31 – 32 3 8 1 14 22 29
Endoplasmic Reticulum 7 7 2 2 1 3 0 2 – 1 0 1 9 21 1 10 – 8 0 2 4 10 6 7
Endosome 16 8 37 29 77 11 5 7 – 1 0 1 10 4 10 5 – 15 28 28 48 31 39 31
Golgi 1 0 0 1 0 0 0 0 – 0 0 0 0 0 0 1 – 4 5 7 8 1 4 3
Mitochondria 76 184 226 206 209 290 449 427 – 378 347 372 3 6 2 12 – 14 130 187 270 239 327 285
Nucleus 3 3 0 3 0 7 11 12 – 4 3 2 3 0 1 2 – 2 1 0 0 0 0 1
Nuclear Periphery 1 1 0 1 0 0 4 5 – 4 3 4 0 0 0 0 – 0 1 1 4 2 3 3
Nucleolus 1 0 1 1 1 0 12 10 – 4 2 3 0 0 0 1 – 0 0 2 1 0 3 2
Peroxisomes 1 0 38 25 39 10 16 10 – 2 0 9 1 4 2 1 – 7 6 6 22 11 11 5
SpindlePole 9 4 26 19 18 11 38 40 – 21 8 19 4 2 4 14 – 16 3 7 11 16 17 12
Vac/Vac Membrane 22 16 25 9 29 13 16 17 – 8 9 9 11 6 12 11 – 11 8 22 3 17 25 20
Unique Cell Count 137 247 334 267 324 319 500 484 398 365 391 176 244 284 79 97 203 295 407 382 516 464
Labelled Cell Count 163 297 420 342 427 387 618 601 482 432 463 191 258 292 96 119 203 295 407 382 516 464


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 4.2 4.1 4.1 3.9 3.3 3.2 3.5 – 2.9 3.2 3.2 5.3 5.2 5.6 5.2 4.6 5.7 5.1 5.1 5.6
Std Deviation (1e-4) 0.9 0.8 1.2 1.5 1.2 1.2 1.3 1.3 – 0.8 1.2 1.1 1.1 0.8 1.3 2.2 1.3 2.1 1.7 0.9 1.6
Intensity Change (Log2) – – – -0.01 -0.07 -0.33 -0.39 -0.25 – -0.51 -0.38 -0.36 0.36 0.33 0.44 0.32 0.16 0.47 0.31 0.3 0.45


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 – 0
Bud Neck – – – – – – – – – – – – 0 – 0
Bud Site – – – – – – – – – – – – 0 – 0
Cell Periphery – – – – – – – – – – – – 0 – 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 – 0
Endosome – – – – – – – – – – – – 0 – 0
Golgi – – – – – – – – – – – – 0 – 0
Mitochondria – – – – – – – – – – – – 0 – 0
Nuclear Periphery – – – – – – – – – – – – 0 – 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 – 0
Peroxisomes – – – – – – – – – – – – 0 – 0
SpindlePole – – – – – – – – – – – – 0 – 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 – 0
Cytoplasm – – – – – – – – – – – – 0 – 0
Nucleus – – – – – – – – – – – – 0 – 0
Vacuole – – – – – – – – – – – – 1.8 – 1.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.9517 0.8204 1.0375 1.2469 0.3491 1.2231 1.4279 1.9096 1.8521 2.512 1.9057 1.7318 2.1138 2.2054 2.2013 1.9475 2.3188 2.3067
Actin 0.0236 0.0042 0.0145 0.0071 0.0058 0.0049 0.0262 0.0039 0.0434 0.0574 0.03 0.0175 0.022 0.0151 0.0183 0.0073 0.0013 0.0124
Bud 0.0016 0.003 0.0044 0.0026 0.0032 0.001 0.0022 0.0041 0.0026 0.0038 0.0064 0.0015 0.0018 0.0025 0.0026 0.0028 0.0007 0.0012
Bud Neck 0.0019 0.0018 0.0097 0.0007 0.0022 0.01 0.0072 0.0021 0.0057 0.0037 0.002 0.0119 0.0056 0.0063 0.0055 0.017 0.0003 0.0046
Bud Periphery 0.0089 0.0091 0.0082 0.0098 0.0086 0.0028 0.0068 0.01 0.0079 0.0108 0.0119 0.0069 0.0031 0.0048 0.0074 0.0143 0.0046 0.0036
Bud Site 0.0043 0.0167 0.0092 0.0031 0.0056 0.0017 0.0141 0.0418 0.0162 0.0072 0.012 0.0024 0.0124 0.0548 0.0155 0.0039 0.0004 0.0021
Cell Periphery 0.0015 0.0021 0.002 0.0011 0.0011 0.0008 0.0072 0.006 0.0058 0.0027 0.0023 0.0017 0.0022 0.0034 0.0036 0.0061 0.0007 0.0015
Cytoplasm 0.0614 0.1348 0.0668 0.0999 0.0659 0.0689 0.0565 0.0985 0.0677 0.0866 0.1094 0.1041 0.0724 0.1273 0.0686 0.1244 0.033 0.0973
Cytoplasmic Foci 0.1288 0.1204 0.1102 0.0629 0.0956 0.1176 0.151 0.1713 0.1362 0.2105 0.1317 0.1898 0.1496 0.1479 0.1359 0.0802 0.1366 0.1642
Eisosomes 0.001 0.0006 0.0017 0.0006 0.0003 0.0005 0.0018 0.0014 0.0029 0.0011 0.0008 0.0008 0.0012 0.0008 0.0022 0.0009 0.0003 0.0008
Endoplasmic Reticulum 0.0083 0.0085 0.0057 0.0036 0.0014 0.0048 0.0047 0.0023 0.0045 0.0035 0.0077 0.0026 0.0098 0.0033 0.0038 0.0023 0.0062 0.0079
Endosome 0.1552 0.1583 0.1154 0.1245 0.177 0.1274 0.1515 0.1308 0.0731 0.0636 0.1342 0.1224 0.183 0.1308 0.1247 0.0619 0.2094 0.1294
Golgi 0.0309 0.0284 0.0338 0.0407 0.0513 0.0387 0.059 0.0449 0.0391 0.0333 0.0591 0.0431 0.0752 0.071 0.0523 0.0517 0.0618 0.0583
Lipid Particles 0.0724 0.0294 0.0365 0.0526 0.0097 0.0384 0.0541 0.0176 0.0223 0.0106 0.0275 0.0301 0.0483 0.0185 0.0188 0.0033 0.038 0.0239
Mitochondria 0.2107 0.1846 0.2603 0.3019 0.3944 0.2401 0.183 0.1872 0.3099 0.1698 0.1228 0.1855 0.2353 0.2024 0.3269 0.4825 0.3665 0.2831
None 0.1701 0.2266 0.2438 0.2274 0.1281 0.2618 0.2122 0.2297 0.1703 0.2516 0.2897 0.1829 0.0949 0.1575 0.1428 0.1132 0.0689 0.1312
Nuclear Periphery 0.0194 0.0022 0.0057 0.0028 0.0006 0.003 0.0021 0.0005 0.0012 0.0005 0.0013 0.0008 0.0043 0.002 0.0008 0.0002 0.0017 0.002
Nucleolus 0.003 0.0035 0.0019 0.0011 0.0009 0.0016 0.0019 0.0027 0.0015 0.001 0.0007 0.0006 0.0014 0.0014 0.001 0.0006 0.0003 0.0005
Nucleus 0.0127 0.0063 0.0037 0.0023 0.0012 0.0019 0.0017 0.0022 0.0026 0.001 0.0011 0.0007 0.0021 0.0019 0.0012 0.0009 0.0006 0.0011
Peroxisomes 0.0462 0.0241 0.0391 0.0365 0.032 0.0563 0.0381 0.028 0.0728 0.07 0.0365 0.0817 0.0513 0.0259 0.055 0.0188 0.0518 0.0645
Punctate Nuclear 0.016 0.0047 0.0034 0.0018 0.0016 0.0028 0.0031 0.0018 0.0049 0.0025 0.0029 0.003 0.0041 0.0044 0.001 0.0002 0.0016 0.0017
Vacuole 0.0162 0.0252 0.0181 0.0105 0.0085 0.0105 0.0124 0.0108 0.0069 0.0065 0.007 0.0069 0.0149 0.0145 0.0085 0.0057 0.0102 0.0055
Vacuole Periphery 0.0058 0.0056 0.006 0.0065 0.005 0.0043 0.0031 0.0023 0.0024 0.0021 0.0029 0.003 0.0051 0.0033 0.0034 0.0019 0.0052 0.0033

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3.3778 3.355 6.4729 8.1598 7.7856 6.7518 9.3287 11.9056 10.1586 10.2161
Translational Efficiency 0.947 0.7488 0.5037 0.5244 0.3878 0.5708 0.4475 0.4342 0.4686 0.4693

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
406 770 166 1020 1366 1479 1838 1502 1772 2249 2004 2522

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 777.72 734.46 897.04 847.02 640.23 747.52 840.08 832.36 671.73 743.05 844.80 838.29
Standard Deviation 102.70 130.38 105.12 111.63 73.13 89.12 97.37 134.12 99.39 105.27 99.29 125.72
Intensity Change Log 2 — -0.082567 0.205921 0.123145 — 0.223522 0.391936 0.378617 — 0.063719 0.292891 0.244122

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002071 0.000959 0.006087 0.011070 0.000434 0.001077 0.003647 0.017061 0.000809 0.001037 0.003849 0.014638
Bud Neck 0.058231 0.030506 0.011778 0.027202 0.035300 0.033732 0.041946 0.036354 0.040554 0.032628 0.039447 0.032652
Bud Site 0.049400 0.025893 0.030494 0.075782 0.012189 0.012832 0.046616 0.103960* 0.020715 0.017304 0.045280 0.092563
Cell Periphery 0.000352 0.000750 0.000458 0.000272 0.000525 0.000425 0.000167 0.000412 0.000485 0.000536 0.000191 0.000355
Cytoplasm 0.182128* 0.049324 0.175820* 0.185078* 0.043429 0.033834 0.293295* 0.134365* 0.075207 0.039137 0.283564* 0.154875*
Cytoplasmic Foci 0.184035* 0.229259* 0.188718* 0.182667* 0.197312* 0.172728 0.138161 0.195868* 0.194270* 0.192083* 0.142348 0.190529*
Eisosomes 0.000234 0.000240 0.000274 0.000206 0.000274 0.000228 0.000136 0.000294 0.000265 0.000232 0.000148 0.000259
Endoplasmic Reticulum 0.004968 0.001604 0.012887 0.005415 0.001011 0.000772 0.005037 0.002881 0.001917 0.001057 0.005687 0.003906
Endosome 0.066927 0.109532 0.078454 0.054684 0.075894 0.098315 0.021230 0.047756 0.073840 0.102155 0.025970 0.050558
Golgi 0.007567 0.118920* 0.012677 0.016528 0.087111 0.094193 0.005028 0.036022 0.068886 0.102659 0.005661 0.028138
Lipid Particles 0.006716 0.011965 0.037179 0.007770 0.025129 0.014789 0.006854 0.005726 0.020910 0.013822 0.009366 0.006553
Mitochondria 0.016383 0.261783* 0.007353 0.022891 0.413459* 0.389902* 0.018209 0.080542 0.322481* 0.346037* 0.017310 0.057226
Mitotic Spindle 0.006750 0.015043 0.020715 0.060494 0.003298 0.015298 0.052945 0.037640 0.004089 0.015211 0.050275 0.046883
None 0.001235 0.006013 0.002842 0.001007 0.001171 0.001453 0.001767 0.001200 0.001186 0.003014 0.001856 0.001122
Nuclear Periphery 0.001322 0.000325 0.003268 0.001329 0.000477 0.000406 0.002401 0.000378 0.000671 0.000378 0.002473 0.000763
Nuclear Periphery Foci 0.000813 0.000304 0.010711 0.002359 0.000190 0.000272 0.002079 0.000587 0.000333 0.000283 0.002794 0.001304
Nucleolus 0.008100 0.003094 0.009319 0.001850 0.001742 0.002539 0.002638 0.000831 0.003198 0.002729 0.003192 0.001243
Nucleus 0.143601* 0.010764 0.158216* 0.056706 0.008409 0.012454 0.191719* 0.031645 0.039384 0.011876 0.188943* 0.041781
Peroxisomes 0.045719 0.077692 0.047042 0.088769 0.060502 0.061794 0.045693 0.152950* 0.057115 0.067237 0.045805 0.126993*
Vacuole 0.207129* 0.032704 0.175624 0.192042 0.019629 0.038910 0.118541 0.109177 0.062589 0.036785 0.123269 0.142691
Vacuole Periphery 0.006319 0.013328 0.010084 0.005880 0.012517 0.014045 0.001892 0.004351 0.011097 0.013799 0.002571 0.004969

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.36 -3.37 -5.00 -6.25 -1.83 -1.83 -7.97 -10.35 -9.66 -8.15 -0.91 -7.38 -11.83 -11.44 -8.81
Bud Neck 3.68 7.31 4.93 1.34 -6.48 0.41 -1.69 0.02 -0.47 2.11 2.23 0.37 2.99 0.56 3.27
Bud Site 3.10 1.95 -2.73 -8.35 -5.92 -0.45 -11.22 -20.10 -20.08 -12.01 1.34 -7.49 -19.20 -21.42 -12.18
Cell Periphery -1.05 -2.51 1.68 1.27 5.85 0.89 4.96 1.63 0.43 -4.69 -0.35 5.08 2.37 1.40 -4.68
Cytoplasm 11.66 0.55 -0.62 -16.98 -1.05 2.59 -36.84 -17.39 -20.05 18.91 8.74 -30.17 -15.76 -26.43 16.52
Cytoplasmic Foci -4.69 0.25 1.34 7.56 0.81 3.39 10.81 1.05 -2.52 -10.58 0.07 10.78 2.26 2.41 -9.96
Eisosomes -0.36 -2.24 1.49 1.68 4.68 2.04 7.34 -0.38 -2.45 -8.03 1.68 7.03 1.10 -0.86 -6.87
Endoplasmic Reticulum 1.84 -4.16 -0.32 -4.89 6.06 1.20 -13.51 -5.68 -6.49 5.90 1.82 -8.58 -4.24 -8.18 5.47
Endosome -6.18 -1.24 2.56 11.09 3.64 -4.65 16.46 8.06 12.93 -10.28 -7.22 15.88 8.40 16.94 -10.18
Golgi -19.52 -1.64 -3.04 18.27 -0.66 -1.26 22.04 12.99 14.55 -13.65 -7.71 21.06 13.47 22.89 -13.80
Lipid Particles -3.07 -4.60 -0.98 2.53 4.45 4.35 9.30 9.60 6.27 1.42 3.85 7.31 9.13 6.56 3.25
Mitochondria -23.42* 3.22 -1.21 23.09 -4.74 1.97 42.68* 33.82* 33.17* -13.37 -2.12 37.23 31.65 40.39* -12.54
Mitotic Spindle -2.83 -2.49 -9.48 -7.94 -4.61 -6.26 -14.53 -12.11 -6.78 3.18 -7.20 -14.50 -15.50 -10.37 0.81
None -2.59 -1.54 0.88 2.77 1.90 -0.70 -2.13 0.07 0.60 1.35 -2.63 -2.72 0.38 2.72 2.58
Nuclear Periphery 3.55 -1.82 -0.37 -5.01 1.71 0.38 -9.71 -0.06 -0.62 11.56 2.02 -9.28 -1.36 -4.20 8.97
Nuclear Periphery Foci 4.92 -5.56 -4.19 -5.62 4.47 -1.33 -6.88 -5.80 -4.29 5.11 1.16 -8.27 -6.35 -6.66 4.19
Nucleolus 2.06 -0.47 2.77 1.92 3.93 -1.32 -2.55 1.75 3.92 8.08 0.50 -0.71 2.99 4.05 7.77
Nucleus 12.86 -0.79 7.18 -13.39 5.82 -2.24 -35.57 -10.74 -8.47 28.46 9.03 -27.12 -2.39 -15.27 27.28
Peroxisomes -4.99 -0.56 -4.75 0.48 -3.31 -0.35 5.33 -12.96 -12.94 -17.56* -2.90 4.66 -12.48 -10.20 -17.17
Vacuole 16.52 1.75 -1.19 -25.49 -2.97 -7.06 -29.39 -22.61 -18.00 0.82 7.54 -15.83 -20.17 -29.96 -5.38
Vacuole Periphery -3.92 -1.30 0.55 7.34 2.28 -1.26 12.34 9.03 11.42 -5.83 -2.81 10.95 8.07 13.50 -5.70
Standard name
Human Ortholog
Description Mitochondrial translational activator specific for the COX3 mRNA; acts together with Pet54p and Pet122p; located in the mitochondrial inner membrane
Localization
Cell Percentages mitochondrion (66%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Pet494

Pet494


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pet494-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available