Standard name | |
---|---|
Human Ortholog | |
Description | ATP-dependent Lon protease; involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 1 | 2 | 4 | 4 | 2 | 0 | 1 | 0 | 11 | 14 | 9 | 5 | 11 | 13 |
Bud | 12 | 12 | 12 | 7 | 12 | 12 | 12 | 17 | – | 18 | 12 | 9 | 4 | 4 | 1 | 19 | 6 | 7 | 3 | 8 | 2 | 4 | 2 | 1 |
Bud Neck | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 2 | 5 | 2 |
Bud Site | 1 | 1 | 4 | 0 | 1 | 5 | 4 | 6 | – | 6 | 7 | 4 | 0 | 0 | 0 | 6 | 6 | 1 | – | – | – | – | – | – |
Cell Periphery | 8 | 5 | 4 | 2 | 2 | 0 | 4 | 1 | – | 2 | 5 | 3 | 3 | 3 | 3 | 9 | 14 | 9 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 4 | 10 | 6 | 8 | 8 | 3 | 7 | – | 3 | 2 | 1 | 6 | 6 | 11 | 17 | 22 | 8 | 0 | 2 | 0 | 1 | 0 | 0 |
Endoplasmic Reticulum | 0 | 2 | 1 | 0 | 1 | 1 | 1 | 1 | – | 4 | 1 | 3 | 0 | 0 | 1 | 4 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 48 | 6 | 24 | 3 | 14 | 3 | 7 | 6 | – | 1 | 0 | 2 | 20 | 19 | 15 | 15 | 17 | 11 | 28 | 30 | 20 | 16 | 29 | 23 |
Golgi | 5 | 0 | 1 | 2 | 5 | 1 | 0 | 2 | – | 0 | 0 | 2 | 55 | 55 | 72 | 4 | 6 | 11 | 27 | 24 | 21 | 18 | 24 | 41 |
Mitochondria | 370 | 220 | 240 | 200 | 218 | 351 | 429 | 543 | – | 465 | 342 | 428 | 133 | 133 | 148 | 17 | 12 | 11 | 384 | 159 | 215 | 163 | 162 | 195 |
Nucleus | 2 | 3 | 1 | 1 | 0 | 2 | 0 | 4 | – | 0 | 1 | 1 | 2 | 2 | 1 | 3 | 9 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 16 | 10 | 11 | 6 | 14 | 26 | 37 | 34 | – | 43 | 36 | 46 | 5 | 6 | 5 | 15 | 7 | 8 | 1 | 0 | 2 | 1 | 3 | 3 |
Nucleolus | 55 | 20 | 19 | 11 | 32 | 52 | 73 | 73 | – | 64 | 30 | 57 | 8 | 8 | 6 | 24 | 14 | 17 | 4 | 3 | 2 | 2 | 3 | 2 |
Peroxisomes | 66 | 38 | 119 | 49 | 36 | 24 | 31 | 20 | – | 11 | 6 | 14 | 16 | 13 | 22 | 68 | 35 | 40 | 37 | 26 | 71 | 34 | 44 | 50 |
SpindlePole | 18 | 8 | 28 | 10 | 8 | 8 | 8 | 17 | – | 4 | 7 | 10 | 13 | 13 | 4 | 22 | 15 | 9 | 17 | 11 | 12 | 13 | 12 | 12 |
Vac/Vac Membrane | 57 | 23 | 10 | 6 | 8 | 12 | 12 | 11 | – | 18 | 5 | 6 | 42 | 43 | 47 | 26 | 12 | 19 | 12 | 11 | 3 | 5 | 3 | 5 |
Unique Cell Count | 491 | 271 | 345 | 245 | 279 | 409 | 492 | 618 | 505 | 359 | 466 | 214 | 215 | 247 | 198 | 141 | 132 | 542 | 301 | 371 | 274 | 307 | 361 | |
Labelled Cell Count | 661 | 354 | 484 | 303 | 359 | 505 | 621 | 742 | 641 | 455 | 588 | 312 | 310 | 338 | 249 | 178 | 156 | 542 | 301 | 371 | 274 | 307 | 361 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 10.0 | 9.5 | 8.5 | 9.2 | 10.1 | 9.6 | 9.3 | 8.6 | – | 8.1 | 7.7 | 8.9 | 10.5 | 10.5 | 10.7 | 8.0 | 8.6 | 11.2 | 10.2 | 11.2 | 11.6 |
Std Deviation (1e-4) | 1.9 | 2.0 | 2.1 | 3.2 | 2.7 | 2.9 | 3.3 | 2.6 | – | 2.6 | 2.4 | 3.0 | 1.9 | 1.9 | 2.3 | 1.9 | 3.0 | 3.2 | 2.6 | 2.6 | 2.3 |
Intensity Change (Log2) | – | – | – | 0.12 | 0.24 | 0.17 | 0.13 | 0.02 | – | -0.06 | -0.14 | 0.07 | 0.31 | 0.31 | 0.34 | -0.08 | 0.02 | 0.4 | 0.27 | 0.4 | 0.45 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | -0.4 | 0.5 | -0.4 | -0.9 | -0.6 | – | 0.1 | -0.1 | -1.4 | -1.1 | -1.1 | -2.5 | 3.0 | 0.4 | 0.9 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.6 | 0 |
Cytoplasm | -0.3 | 0 | -0.8 | -2.6 | -2.0 | – | -2.7 | -2.4 | 0 | -0.1 | -0.1 | 1.0 | 2.9 | 5.1 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -3.3 | -1.0 | -4.6 | -4.2 | -5.1 | – | -5.7 | -5.1 | -5.2 | 1.0 | 0.8 | -0.4 | 0.3 | 1.8 | 0.5 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 9.7 | 9.7 | 10.5 | 0 | 0 | 0 |
Mitochondria | 3.3 | 2.4 | 5.4 | 6.3 | 7.0 | – | 8.6 | 9.0 | 8.2 | -1.8 | -1.9 | -2.4 | -13.7 | -12.2 | -12.0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | -0.5 | 1.2 | 2.0 | 2.7 | 1.6 | – | 3.1 | 3.6 | 3.7 | -0.6 | -0.3 | -0.9 | 2.3 | 0.9 | 1.4 |
Nucleolus | -0.6 | 2.7 | 3.4 | 4.2 | 3.2 | – | 3.5 | 1.5 | 3.2 | -0.9 | -1.0 | -1.8 | 2.7 | 1.8 | 2.7 |
Peroxisomes | -3.8 | -6.2 | -10.0 | -10.5 | -13.2 | – | -12.9 | -11.4 | -12.0 | -7.3 | -7.7 | -7.2 | 0 | -2.1 | -0.9 |
SpindlePole | -2.0 | -2.8 | -4.0 | -4.6 | -3.8 | – | -5.5 | -3.8 | -4.0 | -0.9 | -0.9 | -3.4 | 1.2 | 0.9 | -0.5 |
Vacuole | -0.3 | 0 | 0 | -0.4 | -1.1 | – | 0.5 | -1.4 | -1.6 | 6.6 | 6.7 | 6.6 | 4.6 | 2.7 | 4.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 9.959 | 10.6705 | 10.2788 | 10.1007 | 8.9543 | 10.412 | 9.5216 | 13.3129 | 11.9999 | 11.8646 | 12.8823 | 12.9345 | 3.8127 | 12.8043 | 12.3139 | 10.6606 | 12.0899 | 12.5316 |
Actin | 0.0188 | 0.0048 | 0.0227 | 0.0101 | 0.0007 | 0.0011 | 0.0024 | 0.0013 | 0.0159 | 0.0185 | 0.0291 | 0.009 | 0.0017 | 0.01 | 0.0045 | 0.0189 | 0.0007 | 0.0006 |
Bud | 0.0002 | 0.001 | 0.0001 | 0.0016 | 0 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0002 | 0.0004 | 0 | 0.0005 | 0.0007 | 0.0002 | 0.0008 | 0.0062 | 0 |
Bud Neck | 0.0004 | 0.0001 | 0.0001 | 0.001 | 0 | 0.0001 | 0.0008 | 0.0001 | 0.0001 | 0.0008 | 0.0002 | 0 | 0.0008 | 0.0011 | 0.0013 | 0.0004 | 0 | 0 |
Bud Periphery | 0.0003 | 0.001 | 0.0003 | 0.0012 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0.0006 | 0.0004 | 0.0001 | 0.0011 | 0.0007 | 0.0002 | 0.001 | 0.0337 | 0.0003 |
Bud Site | 0.0005 | 0.0052 | 0.0002 | 0.0083 | 0 | 0.0001 | 0.0126 | 0.0001 | 0.0003 | 0.0007 | 0.0052 | 0.0001 | 0.0019 | 0.026 | 0.0035 | 0.001 | 0.0006 | 0 |
Cell Periphery | 0.0002 | 0.0006 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0012 | 0.0011 | 0.0002 | 0.0001 | 0.0006 | 0.0001 |
Cytoplasm | 0.0023 | 0.0007 | 0 | 0.0038 | 0.0004 | 0 | 0.0043 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0273 | 0.0003 | 0.0001 | 0.0002 | 0 | 0 |
Cytoplasmic Foci | 0.009 | 0.0021 | 0.0007 | 0.0026 | 0.0009 | 0.0006 | 0.0135 | 0.001 | 0.0053 | 0.0074 | 0.0061 | 0.0004 | 0.0065 | 0.0211 | 0.0149 | 0.0068 | 0.0002 | 0.0002 |
Eisosomes | 0.0008 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0002 | 0.0001 | 0.0002 | 0.0011 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0014 | 0.0001 | 0.0001 | 0.0008 | 0.0038 | 0.0001 | 0.002 | 0 | 0 | 0.0001 | 0 | 0.0003 | 0.0083 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0002 |
Endosome | 0.0099 | 0.0053 | 0.0023 | 0.0033 | 0.0105 | 0.0049 | 0.0183 | 0.0017 | 0.0016 | 0.0056 | 0.0009 | 0.0013 | 0.084 | 0.0058 | 0.0084 | 0.0143 | 0.0009 | 0.0013 |
Golgi | 0.0092 | 0.0121 | 0.0098 | 0.0063 | 0.008 | 0.008 | 0.005 | 0.0033 | 0.0037 | 0.0126 | 0.0039 | 0.0083 | 0.0322 | 0.0075 | 0.0131 | 0.0437 | 0.0003 | 0.0025 |
Lipid Particles | 0.024 | 0.0199 | 0.0056 | 0.002 | 0.0153 | 0.0047 | 0.0469 | 0.017 | 0.012 | 0.0449 | 0.0018 | 0.0025 | 0.0361 | 0.0141 | 0.0398 | 0.0029 | 0.0005 | 0.0125 |
Mitochondria | 0.8567 | 0.919 | 0.9333 | 0.937 | 0.9267 | 0.9723 | 0.8555 | 0.9039 | 0.9411 | 0.8721 | 0.927 | 0.9744 | 0.1393 | 0.8803 | 0.8884 | 0.9055 | 0.9455 | 0.9712 |
None | 0.0043 | 0.0001 | 0 | 0.0007 | 0.0003 | 0 | 0.0077 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0046 | 0.0002 | 0 | 0 | 0.0002 | 0 |
Nuclear Periphery | 0.0142 | 0.001 | 0.0103 | 0.0027 | 0.0182 | 0.0002 | 0.004 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.1575 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0004 |
Nucleolus | 0.0028 | 0.0005 | 0.0001 | 0.0006 | 0.0002 | 0.0005 | 0.0016 | 0.0033 | 0.0001 | 0.0003 | 0 | 0.0002 | 0.0617 | 0.0005 | 0.0003 | 0 | 0.0005 | 0 |
Nucleus | 0.0184 | 0.0002 | 0.0001 | 0.0055 | 0.0009 | 0.0003 | 0.0008 | 0.0005 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.2185 | 0.0002 | 0.0001 | 0.0001 | 0.0004 | 0 |
Peroxisomes | 0.0065 | 0.0053 | 0.0024 | 0.0019 | 0.0008 | 0.0012 | 0.0154 | 0.0398 | 0.0175 | 0.025 | 0.0236 | 0.0013 | 0.0011 | 0.0263 | 0.0144 | 0.0022 | 0.0005 | 0.007 |
Punctate Nuclear | 0.0082 | 0 | 0 | 0.0056 | 0.0002 | 0.0001 | 0.0009 | 0 | 0.0001 | 0.0003 | 0 | 0 | 0.1607 | 0.0011 | 0.0002 | 0.0001 | 0 | 0 |
Vacuole | 0.0027 | 0.0057 | 0.0019 | 0.0009 | 0.0043 | 0.0014 | 0.0036 | 0.0112 | 0.0004 | 0.0018 | 0.0002 | 0.0002 | 0.0306 | 0.0009 | 0.0023 | 0.0007 | 0.0039 | 0.0005 |
Vacuole Periphery | 0.009 | 0.0149 | 0.0093 | 0.004 | 0.0083 | 0.004 | 0.0038 | 0.016 | 0.0012 | 0.0081 | 0.0007 | 0.0015 | 0.024 | 0.0011 | 0.0076 | 0.0008 | 0.005 | 0.0031 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 93.5721 | 72.485 | 79.8199 | 86.0914 | 62.0268 | 90.4407 | 85.9686 | 93.7866 | 98.4797 | 82.4413 |
Translational Efficiency | 1.008 | 1.0132 | 0.9415 | 0.9483 | 0.9995 | 1.1568 | 0.9788 | 0.9435 | 0.9437 | 0.9362 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1259 | 1379 | 1014 | 136 | 1349 | 1885 | 2516 | 1827 | 2608 | 3264 | 3530 | 1963 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1103.61 | 1056.14 | 1179.60 | 1326.08 | 886.45 | 1048.83 | 1168.05 | 1120.84 | 991.28 | 1051.92 | 1171.37 | 1135.06 |
Standard Deviation | 159.38 | 194.79 | 166.91 | 233.91 | 162.06 | 173.64 | 190.89 | 173.31 | 193.97 | 182.91 | 184.40 | 185.64 |
Intensity Change Log 2 | — | -0.063429 | 0.096067 | 0.264937 | — | 0.242670 | 0.397991 | 0.338469 | — | 0.080988 | 0.238405 | 0.298155 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001128 | 0.004287 | 0.001853 | 0.040112 | 0.003095 | 0.002404 | 0.001711 | 0.003917 | 0.002145 | 0.003200 | 0.001752 | 0.006425 |
Bud Neck | 0.002797 | 0.001497 | 0.007991 | 0.008714 | 0.000372 | 0.000888 | 0.000968 | 0.001237 | 0.001543 | 0.001146 | 0.002985 | 0.001755 |
Bud Site | 0.004119 | 0.003151 | 0.010859 | 0.034832 | 0.000786 | 0.001234 | 0.002872 | 0.005206 | 0.002395 | 0.002044 | 0.005167 | 0.007258 |
Cell Periphery | 0.000191 | 0.000141 | 0.000286 | 0.000714 | 0.000183 | 0.000133 | 0.000104 | 0.000204 | 0.000187 | 0.000137 | 0.000156 | 0.000240 |
Cytoplasm | 0.000771 | 0.000590 | 0.001837 | 0.002119 | 0.000580 | 0.000500 | 0.000339 | 0.001075 | 0.000672 | 0.000538 | 0.000769 | 0.001148 |
Cytoplasmic Foci | 0.008524 | 0.021440 | 0.055737 | 0.039454 | 0.008315 | 0.018256 | 0.005627 | 0.025296 | 0.008416 | 0.019602 | 0.020021 | 0.026277 |
Eisosomes | 0.000083 | 0.000210 | 0.000088 | 0.000569 | 0.000116 | 0.000127 | 0.000101 | 0.000081 | 0.000100 | 0.000162 | 0.000097 | 0.000115 |
Endoplasmic Reticulum | 0.000134 | 0.000181 | 0.000723 | 0.000905 | 0.000211 | 0.000143 | 0.000054 | 0.000299 | 0.000174 | 0.000159 | 0.000246 | 0.000341 |
Endosome | 0.017964 | 0.016368 | 0.066536 | 0.032025 | 0.004737 | 0.013699 | 0.003952 | 0.024620 | 0.011122 | 0.014827 | 0.021929 | 0.025133 |
Golgi | 0.011204 | 0.035966 | 0.058090 | 0.071130 | 0.017028 | 0.027667 | 0.007804 | 0.055338 | 0.014216 | 0.031173 | 0.022249 | 0.056432 |
Lipid Particles | 0.001466 | 0.007890 | 0.017916 | 0.004783 | 0.004150 | 0.007781 | 0.001705 | 0.002910 | 0.002855 | 0.007827 | 0.006362 | 0.003040 |
Mitochondria | 0.905949* | 0.861717* | 0.658634* | 0.672763* | 0.943982* | 0.896147* | 0.958685* | 0.840989* | 0.925622* | 0.881601* | 0.872494* | 0.829334* |
Mitotic Spindle | 0.005762 | 0.001896 | 0.017577 | 0.014751 | 0.000562 | 0.001681 | 0.001172 | 0.006592 | 0.003073 | 0.001772 | 0.005885 | 0.007157 |
None | 0.000502 | 0.000506 | 0.000683 | 0.000664 | 0.001010 | 0.000364 | 0.000413 | 0.000354 | 0.000765 | 0.000424 | 0.000490 | 0.000375 |
Nuclear Periphery | 0.000271 | 0.000112 | 0.003086 | 0.000306 | 0.000070 | 0.000098 | 0.000087 | 0.000378 | 0.000167 | 0.000104 | 0.000948 | 0.000373 |
Nuclear Periphery Foci | 0.000342 | 0.000210 | 0.004645 | 0.000358 | 0.000229 | 0.000215 | 0.000099 | 0.000325 | 0.000284 | 0.000213 | 0.001405 | 0.000328 |
Nucleolus | 0.000720 | 0.000534 | 0.002116 | 0.000245 | 0.000292 | 0.000223 | 0.000190 | 0.000113 | 0.000499 | 0.000354 | 0.000743 | 0.000122 |
Nucleus | 0.000282 | 0.000536 | 0.001067 | 0.000157 | 0.000870 | 0.000270 | 0.000042 | 0.000079 | 0.000586 | 0.000382 | 0.000336 | 0.000084 |
Peroxisomes | 0.012659 | 0.035241 | 0.019372 | 0.053677 | 0.009596 | 0.023099 | 0.009658 | 0.015162 | 0.011075 | 0.028229 | 0.012449 | 0.017830 |
Vacuole | 0.014806 | 0.004140 | 0.037429 | 0.011489 | 0.001206 | 0.002537 | 0.002389 | 0.006731 | 0.007771 | 0.003214 | 0.012454 | 0.007061 |
Vacuole Periphery | 0.010323 | 0.003387 | 0.033478 | 0.010233 | 0.002610 | 0.002534 | 0.002029 | 0.009094 | 0.006333 | 0.002894 | 0.011063 | 0.009173 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.96 | -1.38 | -4.16 | -3.82 | -4.08 | 0.76 | 1.52 | -0.85 | -2.39 | -3.48 | -1.77 | 0.71 | -4.47 | -3.57 | -5.34 |
Bud Neck | 3.02 | -4.82 | -3.26 | -4.06 | -0.37 | -4.19 | -3.86 | -5.74 | -2.17 | -1.42 | 1.86 | -3.95 | -0.79 | -3.04 | 3.47 |
Bud Site | 0.92 | -3.88 | -3.27 | -3.37 | -2.53 | -1.35 | -3.12 | -5.24 | -4.66 | -2.27 | 0.71 | -3.75 | -4.53 | -4.83 | -1.76 |
Cell Periphery | 1.77 | -2.56 | -3.43 | -3.78 | -2.79 | 1.25 | 1.98 | -0.47 | -2.45 | -3.51 | 2.02 | 1.18 | -1.59 | -3.80 | -2.95 |
Cytoplasm | 1.46 | -1.67 | -2.87 | -3.35 | -0.36 | 0.39 | 1.01 | -1.60 | -3.62 | -5.14 | 0.97 | -0.34 | -2.37 | -4.36 | -1.70 |
Cytoplasmic Foci | -7.32 | -13.91 | -4.53 | -2.60 | 2.16 | -6.12 | 2.32 | -9.26 | -3.48 | -11.98 | -9.40 | -9.00 | -10.82 | -3.70 | -3.35 |
Eisosomes | -6.20 | -0.56 | -3.41 | -2.49 | -3.38 | -0.78 | 1.07 | 2.67 | 4.96 | 2.77 | -5.21 | 0.30 | -1.13 | 3.31 | -1.49 |
Endoplasmic Reticulum | -1.27 | -7.67 | -3.43 | -3.24 | -0.78 | 1.19 | 3.08 | -1.46 | -3.86 | -7.94 | 0.43 | -1.94 | -3.76 | -4.95 | -2.44 |
Endosome | 0.69 | -11.95 | -2.86 | -3.23 | 5.87 | -7.32 | 1.01 | -12.66 | -6.15 | -13.71 | -2.86 | -7.15 | -8.47 | -6.28 | -1.76 |
Golgi | -8.88 | -12.36 | -5.11 | -2.95 | -1.07 | -4.08 | 4.63 | -11.07 | -7.96 | -15.61 | -9.04 | -4.83 | -13.78 | -7.83 | -10.99 |
Lipid Particles | -5.81 | -8.09 | -1.86 | 1.52 | 4.92 | -2.96 | 3.17 | 1.49 | 4.27 | -1.94 | -5.94 | -4.69 | -0.29 | 5.42 | 4.16 |
Mitochondria | 4.87 | 19.68 | 7.50 | 6.05 | -0.43 | 7.03 | -2.74 | 13.30 | 6.88 | 17.27* | 7.92 | 9.19 | 13.23 | 7.00 | 5.66 |
Mitotic Spindle | 2.77 | -4.58 | -2.70 | -4.15 | 0.75 | -2.22 | -2.40 | -7.06 | -5.03 | -6.19 | 1.80 | -3.04 | -3.93 | -6.06 | -1.19 |
None | -0.11 | -4.29 | -2.27 | -2.20 | 0.26 | 2.45 | 2.31 | 2.53 | 0.53 | 2.82 | 2.47 | 2.05 | 2.86 | 2.11 | 5.71 |
Nuclear Periphery | 3.18 | -5.98 | -0.34 | -2.15 | 5.83 | -1.85 | -0.87 | -3.23 | -2.92 | -3.01 | 2.46 | -5.62 | -2.25 | -3.02 | 3.53 |
Nuclear Periphery Foci | 1.81 | -5.09 | -0.11 | -1.71 | 5.07 | 0.11 | 1.01 | -0.67 | -1.47 | -3.56 | 0.92 | -4.38 | -0.45 | -1.81 | 4.30 |
Nucleolus | 0.56 | -1.99 | 2.17 | 1.07 | 2.79 | 0.13 | 0.82 | 1.47 | 1.32 | 1.27 | 0.67 | -1.06 | 3.09 | 1.95 | 3.14 |
Nucleus | -0.58 | -2.97 | 1.14 | 0.88 | 3.65 | 0.99 | 1.54 | 1.47 | 0.96 | -1.48 | 0.53 | 0.86 | 1.77 | 1.39 | 3.40 |
Peroxisomes | -6.60 | -2.87 | -3.73 | -1.64 | -3.11 | -5.41 | -0.03 | -2.60 | 3.30 | -3.14 | -8.42 | -0.96 | -3.53 | 4.52 | -3.03 |
Vacuole | 6.58 | -8.31 | 0.79 | -1.85 | 5.69 | -3.42 | -2.98 | -7.33 | -5.32 | -5.46 | 5.63 | -4.45 | 0.68 | -4.86 | 5.18 |
Vacuole Periphery | 5.63 | -9.51 | 0.02 | -1.65 | 4.99 | 0.13 | 1.12 | -7.08 | -7.57 | -8.54 | 5.15 | -5.17 | -2.84 | -7.50 | 1.81 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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