Standard name
Human Ortholog
Description Mitochondrial tRNA:pseudouridine synthase; catalyzes the formation of pseudouridine at position 32 in mitochondrial tRNAs; contains an N-terminal mitochondrial targeting sequence; PUS9 has a paralog, RIB2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0.11 0 0.11 0.13 0.19 0.14 0.2 0.12 0.19 0.11 0.14 0.12 0.23 0.25 – 0.26 0.3 0 0.05 0 0 0.06 0.05
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.06 0 0 0 0 0 0 0
Endosome 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.22 0.22 0.46 0.53 0.62 0.67 0.8 0.76 0.84 0.77 0.84 0.75 0.08 0.08 0 – 0.07 0.07 0.05 0 0 0.05 0.12 0.11
Nucleus 0.56 0.47 0.5 0.58 0.43 0.32 0.28 0.35 0.31 0.25 0.37 0.35 0.75 0.54 0.49 – 0.3 0.45 0.48 0.55 0.55 0.51 0.35 0.17
Nuclear Periphery 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05
Vac/Vac Membrane 0.25 0.47 0.12 0.09 0.08 0.13 0.1 0 0.05 0 0.07 0.07 0.09 0.21 0.29 – 0.4 0.27 0.32 0.31 0.32 0.34 0.36 0.46
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 2 2
Bud 0 0 0 1 1 1 1 2 0 1 0 0 0 0 0 – 0 0 1 0 0 0 5 8
Bud Neck 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 2
Bud Site 0 0 0 0 0 1 2 0 1 2 0 2 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0
Cytoplasm 9 21 0 10 7 12 20 22 11 17 12 16 30 77 100 – 26 26 8 11 1 7 18 17
Endoplasmic Reticulum 0 1 1 0 0 0 0 0 0 0 0 0 3 3 2 – 6 2 4 0 0 2 3 6
Endosome 12 1 0 0 0 0 0 0 0 0 0 0 0 2 6 – 4 2 2 2 2 3 3 11
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 – 0 0 1 1 1 0 0 1
Mitochondria 36 43 11 47 33 42 114 84 74 68 89 85 20 28 18 – 7 6 11 8 0 9 34 35
Nucleus 92 90 12 52 23 20 40 39 27 22 39 40 187 184 196 – 30 39 102 122 33 103 102 57
Nuclear Periphery 0 2 0 0 1 0 2 0 4 1 2 2 0 1 1 – 0 0 1 1 0 0 1 1
Nucleolus 0 0 0 0 0 0 0 2 0 1 0 2 0 0 0 – 0 0 0 0 0 0 0 1
Peroxisomes 2 0 0 0 0 0 0 0 0 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 0 0 0 1 0 0 1 1 2 1 1 – 1 0 5 2 0 2 3 14
Vac/Vac Membrane 41 90 3 8 4 8 15 4 4 3 7 8 22 71 115 – 40 23 68 70 19 69 104 153
Unique Cell Count 165 192 24 89 53 63 143 111 88 88 106 113 248 341 403 99 86 215 225 61 203 290 330
Labelled Cell Count 193 248 28 119 69 84 194 154 121 115 150 156 265 368 439 114 99 215 225 61 203 290 330


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 4.2 3.8 3.8 3.9 3.7 3.2 3.7 3.5 3.3 3.4 3.4 5.0 5.1 4.7 4.4 5.4 5.4 4.9 5.0 5.5
Std Deviation (1e-4) 1.0 0.8 0.9 0.7 1.1 1.3 1.3 1.5 1.1 1.4 1.3 1.8 1.0 4.9 1.2 0.8 1.7 0.8 1.4 1.7 2.0
Intensity Change (Log2) – – – -0.01 0.04 -0.05 -0.25 -0.07 -0.15 -0.21 -0.17 -0.17 0.37 0.41 0.31 0.21 0.5 0.49 0.34 0.38 0.53


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 0
Bud Neck – – – – – – – – – – – – – 0 0
Bud Site – – – – – – – – – – – – – 0 0
Cell Periphery – – – – – – – – – – – – – 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 0
Endosome – – – – – – – – – – – – – 0 0
Golgi – – – – – – – – – – – – – 0 0
Mitochondria – – – – – – – – – – – – – 0 0
Nuclear Periphery – – – – – – – – – – – – – 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 0
Peroxisomes – – – – – – – – – – – – – 0 0
SpindlePole – – – – – – – – – – – – – 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 0
Cytoplasm – – – – – – – – – – – – – 0 0
Nucleus – – – – – – – – – – – – – 0 0
Vacuole – – – – – – – – – – – – – 2.6 1.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.9816 2.6974 2.1566 2.4569 2.2592 2.1816 2.034 1.2561 1.4762 0.9864 1.0838 1.634 3.2922 3.3261 3.6479 3.2772 2.7827 3.241
Actin 0.0128 0.0004 0.0022 0.0246 0.034 0.0003 0.0576 0.0009 0.0092 0.0003 0.0048 0.0091 0.0251 0.0002 0.0175 0.0001 0.0337 0.0031
Bud 0.0004 0.0002 0.0004 0.0032 0.0024 0.0001 0.0025 0.0005 0.0006 0.0001 0.0023 0.0003 0.0016 0.0002 0.0003 0.0001 0.0001 0.0002
Bud Neck 0.0057 0.0005 0.0003 0.0084 0.0035 0.0024 0.0053 0.0007 0.0005 0.0004 0.0034 0.0023 0.0097 0.0003 0.0014 0.0003 0.0009 0.0035
Bud Periphery 0.0012 0.0002 0.0005 0.005 0.0041 0.0002 0.0041 0.0003 0.0011 0 0.0031 0.0004 0.0032 0.0002 0.0003 0 0.0002 0.0003
Bud Site 0.0005 0.0007 0.0029 0.0043 0.0051 0.0001 0.0094 0.0027 0.0016 0.0001 0.0202 0.0005 0.0034 0.0008 0.0022 0.0002 0.0002 0.0003
Cell Periphery 0.0002 0.0001 0.0001 0.0004 0.0002 0 0.0016 0.0002 0.0002 0 0.0005 0.0001 0.0012 0.0001 0.0001 0 0.0001 0.0001
Cytoplasm 0.02 0.0143 0.0186 0.0043 0.0118 0.0068 0.0131 0.0784 0.0253 0.0791 0.0041 0.0257 0.0095 0.0513 0.037 0.0499 0.0013 0.0221
Cytoplasmic Foci 0.0203 0.0012 0.0037 0.0024 0.011 0.0001 0.0979 0.0017 0.0405 0.0004 0.0092 0.0039 0.0039 0.0012 0.0066 0.0003 0.0008 0.0005
Eisosomes 0.0005 0 0 0.0004 0.0001 0 0.0016 0 0.0001 0 0.0002 0.0001 0.0023 0 0.0001 0 0.0003 0
Endoplasmic Reticulum 0.0056 0.006 0.0011 0.0014 0.0022 0.0008 0.0068 0.0072 0.0028 0.0016 0.0008 0.0035 0.0089 0.0037 0.0036 0.0027 0.0004 0.002
Endosome 0.0172 0.0122 0.0023 0.0086 0.0096 0.0008 0.0985 0.0141 0.0291 0.0015 0.0306 0.0145 0.0229 0.0036 0.0091 0.0016 0.0022 0.0036
Golgi 0.0024 0.0005 0.0007 0.0049 0.007 0 0.0612 0.0003 0.0102 0 0.0615 0.0112 0.0047 0 0.0047 0 0.0034 0.0015
Lipid Particles 0.007 0.0005 0.005 0.0039 0.0108 0 0.0419 0.0002 0.0196 0 0.0134 0.0015 0.0087 0.0001 0.003 0 0.0036 0.001
Mitochondria 0.0037 0.0016 0.0014 0.0178 0.0067 0.0004 0.0676 0.0067 0.0424 0.0013 0.199 0.0114 0.0075 0.0012 0.0027 0.0008 0.0017 0.006
None 0.0163 0.002 0.019 0.0011 0.0127 0.0004 0.0173 0.0028 0.0674 0.0386 0.0006 0.0582 0.0025 0.0109 0.0021 0.0018 0.0009 0.0023
Nuclear Periphery 0.036 0.0306 0.0208 0.0133 0.0251 0.0074 0.0098 0.0397 0.0305 0.029 0.004 0.0154 0.04 0.0312 0.014 0.0151 0.0031 0.015
Nucleolus 0.0084 0.0041 0.004 0.0066 0.0088 0.0098 0.0042 0.0049 0.0066 0.0035 0.0319 0.0072 0.0066 0.0041 0.0055 0.0025 0.0081 0.0069
Nucleus 0.8021 0.9169 0.8993 0.8766 0.8076 0.9658 0.418 0.8198 0.6871 0.835 0.5903 0.809 0.8164 0.8543 0.8663 0.9209 0.9348 0.9136
Peroxisomes 0.0032 0.0001 0.0047 0.0023 0.0087 0 0.0571 0.0003 0.0121 0 0.0111 0.0087 0.0044 0.0001 0.0145 0 0.0019 0.0003
Punctate Nuclear 0.0301 0.0032 0.0113 0.0055 0.0257 0.0032 0.0112 0.003 0.007 0.0073 0.0026 0.0139 0.0048 0.0273 0.0054 0.0026 0.0015 0.0133
Vacuole 0.0041 0.0034 0.0012 0.0038 0.0019 0.0011 0.0077 0.0126 0.0039 0.001 0.0046 0.0023 0.0097 0.0074 0.003 0.0008 0.0005 0.0031
Vacuole Periphery 0.0024 0.0014 0.0005 0.0013 0.001 0.0003 0.0056 0.003 0.002 0.0005 0.0018 0.0008 0.0032 0.0017 0.0005 0.0003 0.0003 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.5046 6.3912 14.7546 12.5966 13.3895 11.1153 11.3087 14.0538 12.6482 13.8104
Translational Efficiency 0.6427 1.5471 0.7565 1.0426 0.8918 0.797 0.8898 0.6959 0.8474 0.8399

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
103 371 2026 105 824 1258 488 276 927 1629 2514 381

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 676.72 711.18 886.11 918.64 733.44 761.03 1000.27 885.73 727.14 749.68 908.27 894.80
Standard Deviation 80.97 102.04 100.94 109.03 88.07 105.45 104.42 118.70 89.11 106.75 111.20 117.04
Intensity Change Log 2 — 0.071656 0.388927 0.440941 — 0.053274 0.447639 0.272188 — 0.062125 0.419761 0.355636

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000048 0.001231 0.000173 0.000884 0.000104 0.000733 0.000583 0.000772 0.000098 0.000847 0.000252 0.000803
Bud Neck 0.001671 0.019893 0.005140 0.004942 0.001683 0.019908 0.004343 0.012004 0.001681 0.019905 0.004985 0.010058
Bud Site 0.000252 0.009451 0.001300 0.003728 0.001249 0.006776 0.003151 0.025951 0.001138 0.007386 0.001660 0.019827
Cell Periphery 0.000182 0.000272 0.000070 0.000181 0.000124 0.000186 0.000106 0.000184 0.000130 0.000205 0.000077 0.000183
Cytoplasm 0.172752* 0.086978 0.019670 0.240943* 0.160481* 0.087275 0.075679 0.245747* 0.161844* 0.087207 0.030542 0.244423*
Cytoplasmic Foci 0.079825 0.034727 0.000122 0.005984 0.023217 0.037707 0.001163 0.013993 0.029507 0.037029 0.000324 0.011786
Eisosomes 0.000112 0.000090 0.000011 0.000062 0.000032 0.000076 0.000037 0.000049 0.000041 0.000079 0.000016 0.000052
Endoplasmic Reticulum 0.000772 0.009331 0.003879 0.009559 0.001408 0.005805 0.007945 0.007010 0.001337 0.006608 0.004668 0.007712
Endosome 0.001857 0.004864 0.000269 0.002546 0.000818 0.003221 0.001086 0.014138 0.000934 0.003595 0.000428 0.010944
Golgi 0.000988 0.001360 0.000020 0.000106 0.000111 0.001872 0.000057 0.008763 0.000208 0.001755 0.000027 0.006378
Lipid Particles 0.006881 0.009576 0.000128 0.001783 0.002337 0.007409 0.000856 0.001673 0.002842 0.007902 0.000270 0.001703
Mitochondria 0.001392 0.008588 0.000679 0.000604 0.000737 0.015838 0.000632 0.002877 0.000810 0.014187 0.000670 0.002250
Mitotic Spindle 0.006978 0.007837 0.000618 0.007081 0.000258 0.012004 0.000976 0.014820 0.001004 0.011055 0.000687 0.012687
None 0.007345 0.021450 0.002257 0.009715 0.009026 0.006718 0.003913 0.007591 0.008840 0.010073 0.002579 0.008176
Nuclear Periphery 0.000684 0.001543 0.001074 0.002099 0.000810 0.002821 0.001762 0.002479 0.000796 0.002530 0.001207 0.002374
Nuclear Periphery Foci 0.000359 0.002072 0.000083 0.005392 0.000346 0.000748 0.000832 0.001958 0.000348 0.001049 0.000228 0.002904
Nucleolus 0.005867 0.016378 0.000638 0.000855 0.002287 0.006747 0.000804 0.000944 0.002685 0.008941 0.000670 0.000920
Nucleus 0.704190* 0.726141* 0.960843* 0.579608* 0.789228* 0.751035* 0.866610* 0.582208* 0.779779* 0.745365* 0.942551* 0.581492*
Peroxisomes 0.002057 0.016259 0.000044 0.000251 0.001103 0.016459 0.000157 0.000994 0.001209 0.016414 0.000066 0.000789
Vacuole 0.005238 0.019413 0.002920 0.123524 0.004401 0.015712 0.029196 0.054137 0.004494 0.016555 0.008020 0.073259
Vacuole Periphery 0.000551 0.002545 0.000064 0.000152 0.000240 0.000950 0.000112 0.001709 0.000275 0.001314 0.000074 0.001280

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.21 -5.12 -7.04 0.19 -6.15 -7.19 -3.02 -7.64 -1.09 0.26 -8.77 -4.30 -10.06 -0.95 -6.08
Bud Neck -5.19 -5.20 -5.71 2.96 -2.34 -10.75 -4.48 -4.27 2.10 -2.67 -11.84 -6.85 -5.18 3.13 -3.05
Bud Site -3.83 -3.10 -5.43 0.32 -4.56 -5.21 -1.80 -3.39 -2.13 -2.50 -6.14 -1.27 -3.98 -2.04 -3.73
Cell Periphery -2.01 3.95 -0.87 1.45 -6.61 -2.93 0.04 -1.43 1.82 -1.84 -3.81 3.50 -2.17 2.14 -7.70
Cytoplasm 3.99 7.79 1.15 -2.71 -6.66 8.46 11.30 -0.36 -5.36 -7.11 9.35 19.07 0.19 -6.01 -11.59
Cytoplasmic Foci 3.27 6.59 5.99 7.04 -4.71 -4.40 10.41 4.03 7.95 -4.29 -2.61 12.32 6.79 10.18 -5.59
Eisosomes 0.56 3.98 0.04 -0.99 -8.65 -9.24 -4.81 -8.21 -1.32 -3.95 -8.06 5.41 -8.10 -1.76 -12.66
Endoplasmic Reticulum -6.05 -12.11 -8.69 -2.00 -6.43 -11.30 -14.97 -11.14 -5.35 1.15 -11.76 -20.32 -13.92 -5.67 -7.64
Endosome -2.84 2.42 -2.87 0.44 -6.19 -6.41 -3.40 -2.86 -1.72 -2.54 -7.32 2.62 -3.37 -1.66 -3.62
Golgi -0.70 1.74 1.43 4.12 -5.48 -3.61 2.26 -1.40 -0.51 -1.42 -4.03 2.90 -1.34 -0.27 -1.47
Lipid Particles -1.44 3.95 2.29 4.23 -8.00 -4.66 3.76 0.31 4.82 -4.01 -5.45 7.73 1.16 6.15 -10.41
Mitochondria -5.18 1.55 1.41 5.87 -0.44 -9.33 -0.03 -1.65 8.39 -1.66 -10.38 0.73 -1.47 9.56 -1.69
Mitotic Spindle -0.27 0.88 -0.95 -0.91 -1.72 -5.46 -1.66 -3.46 -1.07 -2.83 -5.38 -0.55 -3.68 -1.36 -3.61
None -2.91 3.17 -0.20 2.57 -2.31 1.27 4.24 1.21 -0.10 -3.09 -0.51 5.17 1.03 1.51 -4.29
Nuclear Periphery -2.95 -3.22 -6.86 -4.38 -5.98 -7.76 -10.22 -7.35 -2.38 -2.37 -8.06 -6.33 -9.21 -3.62 -7.17
Nuclear Periphery Foci -3.82 1.18 -2.42 -2.09 -2.45 -3.39 -4.71 -3.89 -3.44 -2.79 -5.18 -0.34 -3.80 -3.35 -3.78
Nucleolus -2.99 1.87 1.49 5.60 -1.59 -6.29 4.68 -0.01 4.60 -1.93 -7.31 5.28 0.84 7.20 -2.48
Nucleus -0.34 -9.33 4.16 5.20 13.65 3.66 -2.73 10.40 8.39 11.79 3.70 -17.43 12.00 9.96 22.13
Peroxisomes -4.37 3.30 2.70 4.91 -5.57 -8.19 3.39 0.50 8.28 -2.42 -9.34 4.71 1.35 9.65 -3.31
Vacuole -4.46 0.61 -8.15 -7.51 -8.20 -9.29 -10.11 -11.61 -10.61 -5.94 -10.32 -8.60 -14.13 -12.96 -13.00
Vacuole Periphery -2.14 1.45 1.11 2.78 -5.03 -6.06 1.95 -1.29 0.79 -1.65 -4.81 3.12 -1.06 2.38 -1.92
Standard name
Human Ortholog
Description Mitochondrial tRNA:pseudouridine synthase; catalyzes the formation of pseudouridine at position 32 in mitochondrial tRNAs; contains an N-terminal mitochondrial targeting sequence; PUS9 has a paralog, RIB2, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (34%), cytoplasm (9%), mixed (52%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Pus9

Pus9


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pus9-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available