Standard name | |
---|---|
Human Ortholog | |
Description | Delta-1-pyrroline-5-carboxylate dehydrogenase; nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; deficiency of human homolog ALDH4A1 causes type II hyperprolinemia (HPII), an autosomal recessive inborn error of metabolism; human homolog ALDH4A1 can complement yeast null mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 9 | 6 | 4 | 4 | 0 | 4 | 4 | 0 | 3 | 0 | 0 | 4 | 5 | 6 | 19 | 19 | 18 | 1 | 12 | 3 | 2 | 6 | 12 |
Bud | 2 | 3 | 0 | 1 | 3 | 1 | 2 | 3 | 0 | 0 | 2 | 2 | 2 | 1 | 0 | 6 | 1 | 1 | 0 | 2 | 2 | 0 | 1 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 15 | 3 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 5 | 3 | 3 | 9 | 8 | 7 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 1 | 1 | 3 | 6 | 5 | 6 | 5 | 4 | 3 | 5 | 3 | 5 | 9 | 8 | 2 | 3 | 11 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 2 | 0 | 0 | 0 | 2 | 1 | 1 | 2 | 1 | 1 | 3 | 0 | 2 | 5 | 5 | 8 | 7 | 0 | 1 | 0 | 0 | 0 | 0 |
Endosome | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 3 | 2 | 4 | 0 | 4 | 1 | 1 | 3 | 3 |
Golgi | 5 | 46 | 26 | 26 | 66 | 16 | 16 | 30 | 2 | 17 | 14 | 20 | 75 | 101 | 135 | 100 | 92 | 64 | 9 | 53 | 12 | 11 | 30 | 25 |
Mitochondria | 183 | 335 | 76 | 107 | 151 | 145 | 167 | 230 | 149 | 159 | 236 | 161 | 174 | 199 | 171 | 32 | 34 | 15 | 173 | 337 | 82 | 71 | 128 | 145 |
Nucleus | 0 | 2 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 7 | 5 | 2 | 2 | 8 | 4 | 11 | 5 | 13 | 3 | 16 | 7 | 3 | 2 | 4 | 3 | 5 | 4 | 0 | 1 | 0 | 0 | 1 | 0 |
Nucleolus | 8 | 18 | 1 | 9 | 16 | 29 | 30 | 30 | 15 | 24 | 30 | 18 | 6 | 4 | 6 | 19 | 16 | 23 | 0 | 5 | 0 | 2 | 3 | 2 |
Peroxisomes | 3 | 10 | 3 | 1 | 2 | 2 | 4 | 5 | 1 | 0 | 2 | 1 | 2 | 1 | 3 | 10 | 14 | 13 | 4 | 11 | 8 | 2 | 4 | 5 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | 2 | 1 | 0 | 1 | 0 | 0 | 7 | 6 | 2 | 0 | 1 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 8 | 45 | 12 | 3 | 11 | 12 | 10 | 8 | 5 | 9 | 9 | 14 | 13 | 18 | 13 | 21 | 21 | 26 | 0 | 13 | 2 | 2 | 7 | 4 |
Unique Cell Count | 190 | 397 | 92 | 115 | 198 | 182 | 209 | 273 | 165 | 183 | 254 | 187 | 205 | 242 | 255 | 192 | 184 | 161 | 192 | 449 | 116 | 99 | 190 | 202 |
Labelled Cell Count | 224 | 491 | 131 | 158 | 268 | 217 | 255 | 324 | 192 | 222 | 320 | 235 | 288 | 345 | 360 | 241 | 229 | 196 | 192 | 449 | 116 | 99 | 190 | 202 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 17.0 | 24.8 | 20.8 | 21.5 | 25.4 | 25.4 | 23.0 | 24.2 | 21.3 | 21.6 | 21.2 | 21.5 | 22.3 | 22.3 | 22.0 | 29.3 | 30.4 | 28.7 | 18.8 | 21.5 | 23.0 |
Std Deviation (1e-4) | 4.3 | 6.6 | 5.6 | 5.8 | 6.8 | 7.9 | 7.1 | 7.7 | 6.7 | 7.2 | 7.4 | 7.7 | 5.3 | 5.3 | 6.2 | 12.6 | 12.3 | 12.8 | 5.6 | 5.9 | 6.1 |
Intensity Change (Log2) | – | – | – | 0.05 | 0.29 | 0.29 | 0.15 | 0.22 | 0.03 | 0.05 | 0.03 | 0.05 | 0.1 | 0.1 | 0.08 | 0.49 | 0.55 | 0.46 | -0.15 | 0.05 | 0.14 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.9 | 1.0 | 1.2 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | -0.9 | 0.9 | -4.2 | -4.8 | -4.0 | -6.7 | -4.1 | -5.8 | -3.7 | 1.4 | 2.3 | 4.1 | 3.8 | 3.4 | 1.8 |
Mitochondria | 2.3 | -1.2 | -0.6 | -0.5 | 0.4 | 1.8 | 0.9 | 2.8 | 0.8 | 0.5 | -0.1 | -2.8 | -10.7 | -10.3 | -11.7 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 2.4 | 3.7 | 3.5 | 2.9 | 2.5 | 3.3 | 3.1 | 2.7 | 0 | 0 | 0 | 2.7 | 2.5 | 3.4 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.4 | 1.5 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -2.9 | -2.2 | -1.8 | -2.5 | -3.7 | -3.1 | -2.4 | -3.3 | -1.5 | -1.9 | -1.6 | -2.5 | -0.5 | -0.4 | 0.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 15.9216 | 15.9046 | 15.5074 | 14.9771 | 13.9787 | 14.896 | 13.3457 | 14.0949 | 13.5215 | 12.763 | 10.4607 | 12.9438 | 13.338 | 13.4495 | 13.1127 | 12.1078 | 11.9805 | 12.1076 |
Actin | 0.0022 | 0.0026 | 0.0042 | 0.0006 | 0.0057 | 0.0011 | 0.0196 | 0.0014 | 0.0095 | 0.0171 | 0.0022 | 0.0051 | 0.0055 | 0.0114 | 0.0087 | 0.0011 | 0.0003 | 0.001 |
Bud | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0001 | 0 | 0.0008 | 0.0003 | 0.0006 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0041 | 0.0001 | 0 | 0 | 0 |
Bud Neck | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0057 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0002 | 0.0003 | 0 | 0 | 0 | 0 |
Bud Periphery | 0.0002 | 0.0008 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0019 | 0.0005 | 0.0048 | 0.0004 | 0.0003 | 0.0002 | 0.0003 | 0.0019 | 0.0004 | 0.0001 | 0 | 0 |
Bud Site | 0.0002 | 0.0007 | 0.0003 | 0 | 0.0002 | 0 | 0.0031 | 0.0018 | 0.0012 | 0.0004 | 0.0004 | 0.0001 | 0.0006 | 0.0037 | 0.0001 | 0.0001 | 0 | 0 |
Cell Periphery | 0.0003 | 0.0008 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0003 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0009 | 0.0005 | 0.0002 | 0.0001 | 0 |
Cytoplasm | 0.0001 | 0 | 0.0001 | 0 | 0.0005 | 0 | 0.0026 | 0 | 0.0001 | 0.0004 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0 |
Cytoplasmic Foci | 0.0013 | 0.0011 | 0.0007 | 0.0001 | 0.0099 | 0.0001 | 0.006 | 0.0012 | 0.0012 | 0.0101 | 0.005 | 0.0009 | 0.0093 | 0.0043 | 0.001 | 0.0015 | 0.0001 | 0.0005 |
Eisosomes | 0.0003 | 0.0007 | 0.0005 | 0.0002 | 0.0002 | 0.0002 | 0.0005 | 0.0003 | 0.0019 | 0.0001 | 0.0001 | 0.0004 | 0.0002 | 0.0003 | 0.0003 | 0.0003 | 0.0001 | 0.0002 |
Endoplasmic Reticulum | 0.0004 | 0.0001 | 0.0004 | 0 | 0.0024 | 0 | 0.0008 | 0.0002 | 0.0004 | 0.0005 | 0.0004 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0 |
Endosome | 0.0054 | 0.0044 | 0.0045 | 0.0004 | 0.0046 | 0.0012 | 0.0046 | 0.0028 | 0.0031 | 0.0156 | 0.0169 | 0.0044 | 0.0052 | 0.0049 | 0.0013 | 0.001 | 0.0003 | 0.0026 |
Golgi | 0.0049 | 0.0247 | 0.0175 | 0.0008 | 0.0024 | 0.0081 | 0.004 | 0.01 | 0.0097 | 0.0136 | 0.0131 | 0.005 | 0.0188 | 0.0231 | 0.0084 | 0.0023 | 0.0013 | 0.0164 |
Lipid Particles | 0.0137 | 0.0152 | 0.0057 | 0.0015 | 0.0089 | 0.0083 | 0.0137 | 0.006 | 0.0029 | 0.0072 | 0.0341 | 0.0196 | 0.0183 | 0.0071 | 0.0059 | 0.0181 | 0.0003 | 0.0084 |
Mitochondria | 0.9405 | 0.9236 | 0.9573 | 0.995 | 0.9449 | 0.9672 | 0.9134 | 0.9603 | 0.947 | 0.9042 | 0.8933 | 0.9588 | 0.9148 | 0.8972 | 0.9634 | 0.9551 | 0.9959 | 0.9595 |
None | 0.0001 | 0 | 0.0002 | 0 | 0.0007 | 0 | 0.0029 | 0 | 0.0003 | 0.0004 | 0.0007 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0.0043 | 0.0006 | 0.0022 | 0 | 0.0022 | 0 | 0.0011 | 0.0003 | 0.0009 | 0.0003 | 0.0061 | 0.0004 | 0.0001 | 0.0004 | 0 | 0 | 0 | 0.0001 |
Nucleolus | 0.0004 | 0.0013 | 0.0002 | 0 | 0.0082 | 0.0082 | 0.0004 | 0.0003 | 0.0003 | 0.0001 | 0.0014 | 0.0001 | 0.0002 | 0.0033 | 0.0001 | 0.0001 | 0 | 0 |
Nucleus | 0.0004 | 0.0003 | 0.0006 | 0 | 0.0013 | 0.0011 | 0.0007 | 0.0001 | 0.0005 | 0.0001 | 0.002 | 0.0001 | 0.0001 | 0.0006 | 0 | 0 | 0 | 0 |
Peroxisomes | 0.0046 | 0.0028 | 0.0012 | 0.0006 | 0.0008 | 0.0006 | 0.0064 | 0.0064 | 0.0052 | 0.0171 | 0.0128 | 0.0015 | 0.0226 | 0.0072 | 0.0062 | 0.0171 | 0.0007 | 0.0087 |
Punctate Nuclear | 0.0012 | 0 | 0.0002 | 0 | 0.0008 | 0 | 0.0027 | 0 | 0.0003 | 0.0012 | 0.0076 | 0.0002 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.0033 | 0.0052 | 0.0008 | 0.0002 | 0.0016 | 0.0003 | 0.0035 | 0.0037 | 0.0027 | 0.0027 | 0.0012 | 0.0003 | 0.0007 | 0.0103 | 0.001 | 0.0008 | 0.0001 | 0.0003 |
Vacuole Periphery | 0.0159 | 0.0146 | 0.0031 | 0.0006 | 0.0041 | 0.0033 | 0.0052 | 0.004 | 0.0072 | 0.008 | 0.0017 | 0.0021 | 0.0024 | 0.0186 | 0.0022 | 0.0021 | 0.0007 | 0.002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 50.7643 | 46.8072 | 41.5101 | 51.4874 | 33.53 | 47.8643 | 54.2526 | 57.8468 | 64.9662 | 58.5267 |
Translational Efficiency | 1.2134 | 1.048 | 1.2452 | 1.015 | 1.4025 | 1.6259 | 1.2561 | 1.1299 | 1.1723 | 1.0698 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
139 | 239 | 1146 | 514 | 2039 | 1491 | 1581 | 234 | 2178 | 1730 | 2727 | 748 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1315.52 | 1569.97 | 2149.42 | 1886.66 | 1579.87 | 1458.28 | 2143.23 | 1667.54 | 1563.00 | 1473.71 | 2145.83 | 1818.11 |
Standard Deviation | 263.75 | 349.06 | 448.03 | 556.49 | 358.63 | 399.64 | 459.13 | 440.15 | 359.19 | 394.93 | 454.50 | 532.66 |
Intensity Change Log 2 | — | 0.255104 | 0.708314 | 0.520201 | — | -0.115538 | 0.439981 | 0.077916 | — | 0.064727 | 0.568111 | 0.295767 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001268 | 0.006275 | 0.002022 | 0.007336 | 0.001970 | 0.005157 | 0.004386 | 0.007695 | 0.001925 | 0.005311 | 0.003392 | 0.007449 |
Bud Neck | 0.002689 | 0.002181 | 0.001943 | 0.003964 | 0.000712 | 0.001677 | 0.002930 | 0.012259 | 0.000838 | 0.001747 | 0.002515 | 0.006559 |
Bud Site | 0.002814 | 0.001242 | 0.000790 | 0.006125 | 0.000443 | 0.007023 | 0.001809 | 0.038812 | 0.000594 | 0.006224 | 0.001381 | 0.016350 |
Cell Periphery | 0.000116 | 0.000121 | 0.000072 | 0.000339 | 0.000043 | 0.000287 | 0.000108 | 0.002095 | 0.000048 | 0.000264 | 0.000093 | 0.000888 |
Cytoplasm | 0.000374 | 0.000562 | 0.000176 | 0.000460 | 0.000122 | 0.011825 | 0.000214 | 0.005170 | 0.000139 | 0.010269 | 0.000198 | 0.001933 |
Cytoplasmic Foci | 0.023893 | 0.024269 | 0.023393 | 0.014076 | 0.018131 | 0.026306 | 0.023130 | 0.037434 | 0.018499 | 0.026025 | 0.023240 | 0.021383 |
Eisosomes | 0.000349 | 0.001378 | 0.000464 | 0.000784 | 0.001028 | 0.000721 | 0.000828 | 0.000623 | 0.000984 | 0.000811 | 0.000675 | 0.000733 |
Endoplasmic Reticulum | 0.000186 | 0.000257 | 0.000076 | 0.000555 | 0.000062 | 0.000185 | 0.000098 | 0.001244 | 0.000070 | 0.000195 | 0.000089 | 0.000771 |
Endosome | 0.007366 | 0.006291 | 0.001533 | 0.006967 | 0.001001 | 0.005157 | 0.001490 | 0.028639 | 0.001407 | 0.005314 | 0.001508 | 0.013747 |
Golgi | 0.016135 | 0.031920 | 0.020603 | 0.047111 | 0.012503 | 0.030111 | 0.014916 | 0.048685 | 0.012735 | 0.030361 | 0.017306 | 0.047604 |
Lipid Particles | 0.007905 | 0.008343 | 0.007926 | 0.006559 | 0.006924 | 0.010007 | 0.008487 | 0.021097 | 0.006987 | 0.009777 | 0.008251 | 0.011107 |
Mitochondria | 0.881602* | 0.876272* | 0.875138* | 0.848690* | 0.936030* | 0.867870* | 0.904077* | 0.695838* | 0.932557* | 0.869030* | 0.891916* | 0.800873* |
Mitotic Spindle | 0.001422 | 0.001121 | 0.004410 | 0.003985 | 0.001105 | 0.001909 | 0.003053 | 0.012270 | 0.001126 | 0.001800 | 0.003623 | 0.006577 |
None | 0.001455 | 0.002180 | 0.005265 | 0.003815 | 0.003277 | 0.002600 | 0.003405 | 0.003374 | 0.003161 | 0.002542 | 0.004187 | 0.003677 |
Nuclear Periphery | 0.000186 | 0.000250 | 0.003644 | 0.001365 | 0.000331 | 0.000334 | 0.001487 | 0.002010 | 0.000322 | 0.000323 | 0.002394 | 0.001567 |
Nuclear Periphery Foci | 0.000354 | 0.000252 | 0.001111 | 0.000688 | 0.000238 | 0.000398 | 0.000563 | 0.001406 | 0.000245 | 0.000378 | 0.000793 | 0.000912 |
Nucleolus | 0.001439 | 0.001380 | 0.001575 | 0.000976 | 0.000719 | 0.000769 | 0.001496 | 0.002050 | 0.000765 | 0.000854 | 0.001529 | 0.001312 |
Nucleus | 0.000303 | 0.001604 | 0.000096 | 0.000238 | 0.000074 | 0.001777 | 0.000099 | 0.000650 | 0.000088 | 0.001753 | 0.000098 | 0.000367 |
Peroxisomes | 0.026456 | 0.025149 | 0.003028 | 0.005849 | 0.007655 | 0.010582 | 0.006815 | 0.012627 | 0.008855 | 0.012595 | 0.005224 | 0.007970 |
Vacuole | 0.012681 | 0.004893 | 0.012713 | 0.010358 | 0.003236 | 0.008378 | 0.007407 | 0.031795 | 0.003839 | 0.007896 | 0.009637 | 0.017065 |
Vacuole Periphery | 0.011007 | 0.004058 | 0.034024 | 0.029759 | 0.004395 | 0.006928 | 0.013201 | 0.034227 | 0.004817 | 0.006532 | 0.021951 | 0.031157 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.23 | -1.33 | -3.21 | -0.45 | -2.82 | -2.84 | -2.72 | -2.54 | -1.05 | -1.43 | -3.36 | -2.19 | -3.62 | -1.26 | -2.68 |
Bud Neck | 0.31 | 0.46 | -0.69 | -1.46 | -1.71 | -4.24 | -5.00 | -4.54 | -4.15 | -3.62 | -3.90 | -5.46 | -4.94 | -4.14 | -3.45 |
Bud Site | 0.79 | 1.03 | -1.24 | -2.45 | -2.69 | -5.06 | -2.84 | -4.88 | -3.99 | -4.70 | -4.98 | -2.41 | -5.50 | -3.31 | -5.21 |
Cell Periphery | -0.15 | 1.76 | -3.44 | -3.26 | -4.37 | -5.87 | -2.64 | -3.55 | -3.11 | -3.43 | -5.97 | -2.78 | -4.48 | -3.25 | -4.23 |
Cytoplasm | -1.03 | 2.16 | -0.74 | 0.53 | -3.15 | -5.95 | -3.63 | -2.38 | 2.42 | -2.34 | -5.97 | -3.03 | -2.68 | 4.63 | -2.59 |
Cytoplasmic Foci | -0.06 | 0.08 | 1.51 | 2.41 | 3.48 | -3.68 | -2.24 | -3.18 | -1.78 | -2.33 | -3.62 | -2.54 | -1.13 | 1.66 | 0.67 |
Eisosomes | -4.08 | -1.29 | -3.15 | 2.26 | -2.75 | 3.50 | 2.39 | 3.44 | 0.81 | 1.80 | 2.02 | 4.50 | 2.47 | 0.74 | -0.66 |
Endoplasmic Reticulum | -0.59 | 2.01 | -2.81 | -1.85 | -3.99 | -5.29 | -2.10 | -4.64 | -4.13 | -4.49 | -4.94 | -1.49 | -6.06 | -4.87 | -5.90 |
Endosome | 0.35 | 2.34 | 0.12 | -0.31 | -3.52 | -5.94 | -1.49 | -5.93 | -4.99 | -5.82 | -5.76 | -0.32 | -6.67 | -4.37 | -6.67 |
Golgi | -2.04 | -0.85 | -4.31 | -1.91 | -4.78 | -6.63 | -1.48 | -4.70 | -2.33 | -4.38 | -7.09 | -3.04 | -7.83 | -3.56 | -6.83 |
Lipid Particles | -0.07 | 0.01 | 0.31 | 0.54 | 0.72 | -2.15 | -1.31 | -2.27 | -1.75 | -2.01 | -2.06 | -1.30 | -1.72 | -0.53 | -1.21 |
Mitochondria | 0.21 | 0.29 | 1.38 | 1.54 | 2.16 | 9.11 | 5.40 | 10.22 | 7.07 | 8.78 | 9.06 | 7.84 | 11.61 | 5.43 | 8.01 |
Mitotic Spindle | 0.61 | -2.82 | -2.20 | -2.56 | 0.28 | -1.35 | -2.77 | -2.80 | -2.55 | -2.31 | -1.27 | -3.68 | -3.59 | -3.04 | -1.92 |
None | -2.37 | -10.30 | -6.87 | -5.10 | 3.82 | 2.23 | -0.63 | -0.17 | -1.28 | 0.07 | 2.30 | -5.09 | -1.87 | -3.45 | 1.85 |
Nuclear Periphery | -1.18 | -4.98 | -5.17 | -4.89 | 3.12 | -0.08 | -5.46 | -4.68 | -4.67 | -1.26 | -0.04 | -6.51 | -6.39 | -6.42 | 2.24 |
Nuclear Periphery Foci | 0.80 | -4.00 | -1.60 | -2.30 | 1.61 | -2.50 | -3.64 | -4.24 | -3.58 | -2.95 | -2.32 | -6.37 | -4.22 | -3.28 | -0.77 |
Nucleolus | 0.02 | -0.15 | 0.43 | 0.51 | 1.14 | -0.38 | -2.54 | -1.32 | -1.25 | -0.55 | -0.54 | -3.10 | -1.24 | -1.00 | 0.41 |
Nucleus | -0.87 | 1.38 | 0.27 | 0.90 | -1.40 | -3.61 | -1.00 | -3.68 | 2.30 | -3.54 | -3.65 | -0.40 | -3.13 | 2.99 | -3.10 |
Peroxisomes | 0.12 | 2.49 | 2.16 | 3.59 | -1.61 | -1.80 | 0.72 | -1.80 | -0.69 | -2.13 | -2.18 | 3.21 | 0.51 | 2.33 | -1.82 |
Vacuole | 1.48 | -0.01 | 0.43 | -2.77 | 1.26 | -4.75 | -4.89 | -5.51 | -4.45 | -4.68 | -3.99 | -7.01 | -6.67 | -4.35 | -3.69 |
Vacuole Periphery | 1.60 | -4.77 | -3.32 | -6.70 | 0.97 | -2.88 | -7.09 | -6.27 | -5.68 | -4.30 | -2.07 | -13.30 | -8.78 | -8.10 | -2.88 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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