Standard name
Human Ortholog
Description Metalloprotease subunit of 19S regulatory particle; part of 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.09 0.11 0.09 0.08 0.05 0.12 0.07 0.07 0.17 0.19 0.13 0.08 0.09 0 0 0 0 0.08 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0.05 0 0 0.12 0 0.08 0 0.13 0.07 0.05 0.07 0.09 0.08 0 0.06 0.08 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.81 0.79 0.81 0.74 0.48 0.46 0.47 0.46 0.45 0.44 0.47 0.43 0.56 0.39 0.35 0.4 0.48 0.39 0.83 0.81 0.74 0.73 0.61 0.53
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.06 0.05 0.06 0 0.05 0 0.09 0 0.15 0.05 0.07 0.09 0.06 0 0 0.05 0.08 0.05 0.08 0.07 0.16 0.06 0.08 0.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.13 0.17 0.11 0.19 0.49 0.45 0.36 0.42 0.27 0.37 0.32 0.38 0.28 0.35 0.39 0.42 0.33 0.42 0.06 0.08 0.08 0.13 0.15 0.23
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 2 1 2 1 1 6 0 0 0 3 0 0 0 0 0 0 0 1 1
Bud 0 0 0 0 0 0 0 0 3 0 1 1 0 0 0 0 0 0 3 3 3 5 7 6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 3 0 0 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 1 0 1 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5 10 2 4 3 14 18 17 14 10 32 23 15 38 48 33 23 24 0 0 0 13 23 11
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 1 2 1 0 0 0 13 12 15 0 1 0 0 2 1
Endosome 0 0 0 0 0 1 0 0 0 0 0 1 0 0 1 1 0 0 0 1 1 2 1 3
Golgi 0 1 9 0 4 19 6 15 2 24 18 15 14 20 20 9 18 21 0 0 0 2 2 4
Mitochondria 2 0 0 1 1 0 0 1 16 4 1 5 0 0 0 5 0 1 0 1 0 1 2 3
Nucleus 238 221 157 77 62 73 76 86 76 80 128 133 120 86 88 103 145 101 296 275 192 279 186 185
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 1 0 1 1 1 1 1 3
Nucleolus 19 13 12 4 7 2 15 6 25 10 19 29 12 8 9 13 23 14 27 23 40 23 24 34
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 1 0 0 1
Vac/Vac Membrane 39 46 21 20 64 72 59 79 46 68 87 117 60 78 96 109 100 109 22 27 19 48 47 81
Unique Cell Count 293 278 195 104 130 159 163 188 170 182 274 308 213 221 249 258 302 260 357 340 261 383 305 348
Labelled Cell Count 303 291 201 106 141 184 175 207 186 198 296 328 221 230 265 287 322 285 357 340 261 383 305 348


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 49.8 36.0 37.0 32.8 27.1 30.9 29.1 27.9 24.5 27.0 26.5 25.0 39.9 41.6 39.1 33.1 41.1 38.6 37.9 40.4 41.4
Std Deviation (1e-4) 10.8 9.3 8.2 7.6 8.5 8.7 7.4 6.8 7.8 7.0 7.8 6.9 10.4 11.6 11.2 13.0 14.3 16.4 11.1 13.0 13.7
Intensity Change (Log2) – – – -0.17 -0.45 -0.26 -0.35 -0.41 -0.59 -0.45 -0.48 -0.56 0.11 0.17 0.08 -0.16 0.15 0.06 0.03 0.13 0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 3.5 4.1 3.6 3.4 2.5 4.4 3.2 3.0 5.6 6.0 4.6 3.3 3.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 3.2 0 3.4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 -0.7 2.5 -0.4 1.4 -1.9 2.9 0.9 0.1 0.9 1.8 1.4 -0.6 0.6 1.5
Mitochondria 0 0 0 0 0 4.4 0 0 0 0 0 0 0 0 0
Nucleus -1.3 -6.2 -6.8 -6.7 -7.1 -7.1 -7.3 -7.4 -8.3 -5.2 -8.6 -9.5 -8.7 -7.2 -8.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -0.8 -0.3 -2.4 1.1 -1.4 2.7 -0.3 0.3 1.3 -0.2 -1.2 -1.3 -0.5 0.6 -0.3
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 2.0 7.7 7.3 5.8 7.0 4.0 6.1 5.3 6.7 4.4 5.9 6.6 7.3 5.7 7.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 39.2929 42.7244 39.5042 36.4743 33.1268 40.1131 39.2892 45.1596 40.7359 41.7883 39.2451 42.1715 31.9638 37.1065 36.2877 36.2401 31.9748 36.2336
Actin 0.0002 0 0 0 0 0 0.0332 0 0.0039 0.0002 0.0001 0.0019 0.0144 0 0.0001 0.0344 0.0001 0.0009
Bud 0 0 0 0 0 0 0.0001 0 0.0001 0.0001 0 0.0001 0.0004 0 0 0 0 0.0001
Bud Neck 0 0 0 0 0.0001 0.0005 0.0004 0 0.0001 0.0001 0.0001 0.0005 0.0005 0.0003 0 0.0001 0.0001 0.0008
Bud Periphery 0 0 0 0 0 0 0.0001 0 0.0001 0 0 0.0001 0.0004 0 0 0 0 0.0001
Bud Site 0 0 0 0 0 0 0.0002 0 0.0002 0.0001 0 0.0001 0.0014 0 0 0.0001 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0.0001 0 0.0001 0.0003 0 0 0 0.0001 0.0001
Cytoplasm 0.0017 0.0008 0.0002 0.0005 0.0007 0.0003 0.0079 0.0002 0.0038 0.0015 0.0003 0.0006 0.0067 0.0023 0.0004 0.0003 0.0004 0.0006
Cytoplasmic Foci 0.0002 0 0 0 0 0 0.0003 0 0.0014 0.0195 0 0.0031 0.0216 0 0.0001 0.0001 0 0.0003
Eisosomes 0 0 0 0 0 0 0.0001 0 0 0 0 0 0.0001 0 0 0.0001 0 0
Endoplasmic Reticulum 0.0013 0 0 0 0 0 0.0005 0 0.0007 0 0 0.001 0.0027 0.0001 0 0 0 0.0002
Endosome 0.0004 0 0 0 0 0 0.0013 0 0.0018 0.0001 0 0.0046 0.0119 0 0 0 0 0.0016
Golgi 0 0 0 0 0 0 0.0015 0 0.0005 0.0001 0 0.0015 0.0026 0 0 0.0001 0 0.0002
Lipid Particles 0.0001 0 0 0 0 0 0.0002 0 0.0001 0.0011 0 0.002 0.0074 0 0.0001 0.0001 0 0.0002
Mitochondria 0.0008 0.0004 0.0004 0.0004 0.0004 0.0002 0.0011 0.0008 0.0014 0.002 0.0007 0.0013 0.004 0.0005 0.0006 0.0007 0.0008 0.0003
None 0.0002 0 0 0 0 0 0.0008 0 0.0003 0.0011 0 0.0002 0.004 0 0.0001 0 0 0.0003
Nuclear Periphery 0.0143 0.0107 0.0126 0.0171 0.0405 0.0072 0.0127 0.019 0.025 0.0162 0.0479 0.0089 0.0361 0.0197 0.0158 0.0305 0.0123 0.0079
Nucleolus 0.0079 0.0072 0.004 0.0031 0.0187 0.0184 0.0091 0.0062 0.0045 0.0265 0.0403 0.019 0.0168 0.011 0.0079 0.0048 0.0677 0.0235
Nucleus 0.9716 0.9803 0.9824 0.9783 0.9378 0.9725 0.9273 0.9731 0.9527 0.9195 0.909 0.9525 0.8478 0.9653 0.9741 0.9269 0.9164 0.9589
Peroxisomes 0 0 0 0 0 0 0.0001 0 0.0003 0.0001 0 0.0005 0.0076 0 0 0.0009 0 0.0001
Punctate Nuclear 0.0007 0.0001 0 0.0001 0.0002 0.0001 0.0023 0.0002 0.0007 0.0105 0.0002 0.0008 0.0079 0.0001 0.0002 0.0001 0.0003 0.0015
Vacuole 0.0002 0.0001 0.0001 0.0001 0.0005 0.0003 0.0003 0.0001 0.0011 0.0003 0.0003 0.0004 0.0023 0.0002 0.0001 0.0001 0.0004 0.0017
Vacuole Periphery 0.0006 0.0002 0.0002 0.0003 0.0009 0.0002 0.0004 0.0003 0.0012 0.0008 0.0011 0.0008 0.0029 0.0004 0.0003 0.0007 0.0012 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 68.8879 62.1171 85.0346 90.2513 58.2497 74.238 82.8396 90.6272 102.2435 73.6943
Translational Efficiency 2.5112 2.7236 1.7332 2.171 2.4623 2.8296 2.1541 2.0954 2.0565 2.0169

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1911 1032 2193 1764 2102 2176 2429 1684 4013 3208 4622 3448

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2332.22 2298.25 3005.91 3193.12 2339.17 2737.32 3375.35 3539.14 2335.86 2596.07 3200.06 3362.12
Standard Deviation 583.40 668.94 578.79 740.28 465.17 528.39 711.88 723.86 524.82 612.70 677.72 752.46
Intensity Change Log 2 — -0.021168 0.366098 0.453263 — 0.226767 0.529040 0.597402 — 0.108303 0.449989 0.527239

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000929 0.001120 0.000822 0.000933 0.000900 0.000858 0.000720 0.000977 0.000914 0.000942 0.000768 0.000955
Bud Neck 0.012598 0.016289 0.021751 0.026266 0.015939 0.015996 0.021034 0.030745 0.014348 0.016090 0.021374 0.028454
Bud Site 0.000533 0.000946 0.000619 0.002129 0.000590 0.000842 0.000510 0.001033 0.000563 0.000875 0.000562 0.001594
Cell Periphery 0.000123 0.000222 0.000059 0.000176 0.000100 0.000105 0.000081 0.000126 0.000111 0.000143 0.000071 0.000151
Cytoplasm 0.006382 0.005767 0.000345 0.004371 0.000743 0.000771 0.000856 0.001983 0.003428 0.002378 0.000613 0.003204
Cytoplasmic Foci 0.000105 0.000629 0.000072 0.001306 0.000141 0.000135 0.000252 0.000257 0.000124 0.000294 0.000166 0.000794
Eisosomes 0.000025 0.000031 0.000031 0.000021 0.000036 0.000036 0.000022 0.000018 0.000031 0.000034 0.000026 0.000020
Endoplasmic Reticulum 0.002082 0.000781 0.001030 0.001037 0.002016 0.001439 0.001116 0.000837 0.002047 0.001227 0.001075 0.000939
Endosome 0.000097 0.000089 0.000033 0.000426 0.000080 0.000107 0.000046 0.000081 0.000088 0.000101 0.000040 0.000258
Golgi 0.000065 0.000118 0.000059 0.000285 0.000068 0.000071 0.000064 0.000105 0.000066 0.000086 0.000062 0.000197
Lipid Particles 0.000016 0.000037 0.000005 0.000135 0.000015 0.000029 0.000011 0.000014 0.000015 0.000032 0.000008 0.000076
Mitochondria 0.002215 0.002992 0.002679 0.004261 0.002284 0.001986 0.002479 0.004592 0.002251 0.002310 0.002574 0.004422
Mitotic Spindle 0.000117 0.000783 0.000126 0.007790 0.000680 0.000700 0.000398 0.001921 0.000412 0.000727 0.000269 0.004923
None 0.002716 0.006986 0.000700 0.003484 0.001107 0.001485 0.000748 0.001405 0.001873 0.003255 0.000725 0.002469
Nuclear Periphery 0.002729 0.001631 0.001272 0.008359 0.001679 0.001087 0.002541 0.005822 0.002179 0.001262 0.001939 0.007120
Nuclear Periphery Foci 0.000732 0.001211 0.000035 0.001779 0.000199 0.000150 0.000462 0.000718 0.000452 0.000491 0.000260 0.001261
Nucleolus 0.047326 0.053644 0.035494 0.027204 0.071371 0.074162 0.022627 0.026284 0.059921 0.067561 0.028732 0.026755
Nucleus 0.918937* 0.904157* 0.933690* 0.907715* 0.899368* 0.897697* 0.945095* 0.921460* 0.908687* 0.899775* 0.939684* 0.914428*
Peroxisomes 0.000300 0.000619 0.000558 0.000475 0.000614 0.000514 0.000237 0.000437 0.000464 0.000548 0.000389 0.000456
Vacuole 0.000851 0.001033 0.000293 0.000960 0.000724 0.000686 0.000333 0.000573 0.000784 0.000798 0.000314 0.000771
Vacuole Periphery 0.001122 0.000914 0.000328 0.000889 0.001348 0.001144 0.000368 0.000611 0.001240 0.001070 0.000349 0.000753

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.89 1.14 -1.70 2.69 -2.94 0.11 3.80 -3.92 -2.64 -7.86 -1.32 3.55 -4.03 -1.17 -7.82
Bud Neck -4.23 -13.50 -15.68 -9.99 -5.29 -0.88 -8.23 -14.46 -13.63 -9.05 -3.71 -15.12 -21.02 -17.14 -10.30
Bud Site -3.67 -2.32 -5.76 -3.89 -5.29 -2.48 0.22 -4.09 -0.84 -4.28 -3.73 -1.53 -6.90 -3.71 -6.56
Cell Periphery -3.02 3.16 -2.25 1.53 -6.76 -0.43 0.97 -1.46 -0.91 -2.21 -2.04 2.84 -2.75 -0.32 -5.82
Cytoplasm 0.32 4.99 1.49 1.46 -9.38 -0.90 -0.35 -4.03 -2.62 -4.42 1.11 4.77 -0.01 -1.69 -10.05
Cytoplasmic Foci -2.52 0.87 -3.37 -0.38 -3.47 -0.49 -0.51 -1.29 -1.06 -0.53 -2.57 -0.38 -3.56 -1.57 -2.98
Eisosomes -2.80 -6.18 -0.62 2.58 6.60 0.24 7.08 8.47 10.85 2.06 -2.42 2.28 6.53 9.43 5.88
Endoplasmic Reticulum 5.32 4.47 4.33 -1.79 0.18 3.43 6.36 8.72 6.26 3.21 5.42 7.21 7.98 4.04 1.97
Endosome -0.80 3.56 -3.10 -2.82 -4.04 -1.53 2.46 -1.04 0.96 -2.76 -1.58 4.30 -3.26 -2.05 -4.55
Golgi -3.61 -0.69 -4.76 -3.12 -4.70 -0.64 0.13 -4.87 -4.08 -5.36 -2.68 -0.18 -5.80 -4.46 -5.81
Lipid Particles -1.37 1.93 -3.27 -0.37 -3.57 -1.47 0.79 -0.85 1.33 -1.35 -2.02 1.66 -3.35 -0.08 -3.74
Mitochondria -5.55 -6.74 -14.23 -7.80 -9.15 2.30 -3.67 -15.11 -17.00 -12.84 -1.21 -7.19 -20.66 -19.19 -15.64
Mitotic Spindle -1.69 -0.66 -6.99 -5.91 -6.96 -0.12 0.89 -2.56 -2.49 -3.15 -1.21 0.76 -7.25 -6.51 -7.57
None -3.17 3.53 -1.76 2.38 -7.78 -1.02 1.40 -1.87 -0.19 -5.03 -2.60 3.79 -2.52 1.00 -9.00
Nuclear Periphery 1.96 3.60 -3.72 -4.60 -5.23 2.26 -1.84 -5.99 -6.78 -4.42 3.47 0.64 -6.63 -8.02 -6.74
Nuclear Periphery Foci -0.85 1.76 -1.11 0.20 -3.94 0.20 -0.51 -1.70 -1.76 -0.94 -0.47 1.05 -1.93 -1.26 -3.07
Nucleolus -1.69 3.08 5.39 6.12 2.60 -0.57 13.42 11.34 12.24 -2.19 -2.48 12.14 12.20 13.86 0.35
Nucleus 3.36 -1.91 5.31 1.32 7.46 0.70 -9.89 -0.97 -1.67 8.85 3.17 -8.58 3.15 -0.11 11.51
Peroxisomes -4.41 -4.51 -4.39 1.27 1.00 0.93 2.82 1.25 0.78 -4.16 -0.92 0.72 -0.27 1.16 -1.85
Vacuole -1.52 4.09 -3.00 -1.96 -4.63 -0.81 3.57 0.08 0.93 -5.04 -1.32 5.42 -2.85 -1.60 -5.54
Vacuole Periphery 0.88 3.76 1.77 1.38 -1.59 0.39 4.32 3.01 2.38 -1.83 0.54 5.73 3.31 2.58 -2.28
Standard name
Human Ortholog
Description Metalloprotease subunit of 19S regulatory particle; part of 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages nucleus (96%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-5

Rpn11

Rpn11


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpn11-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available