Standard name | |
---|---|
Human Ortholog | |
Description | Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 14 | 24 | 47 | 51 | 52 | 28 | 61 | 32 | – | 59 | 47 | 51 | 52 | 53 | 44 | 40 | 12 | 15 | 6 | 15 | 15 | 8 | 11 | 18 |
Bud | 8 | 23 | 10 | 17 | 8 | 8 | 24 | 25 | – | 15 | 21 | 12 | 5 | 5 | 1 | 3 | 5 | 3 | 2 | 2 | 1 | 0 | 1 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 1 | 0 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 78 | 95 | 92 | 124 | 85 | 72 | 81 | 65 | – | 79 | 67 | 92 | 344 | 344 | 357 | 122 | 71 | 78 | 3 | 9 | 13 | 24 | 41 | 40 |
Cytoplasm | 125 | 99 | 45 | 43 | 60 | 132 | 177 | 227 | – | 187 | 148 | 181 | 48 | 48 | 45 | 167 | 89 | 94 | 15 | 24 | 1 | 1 | 9 | 7 |
Endoplasmic Reticulum | 410 | 122 | 253 | 175 | 176 | 175 | 222 | 205 | – | 158 | 114 | 193 | 244 | 246 | 277 | 3 | 7 | 2 | 524 | 298 | 320 | 202 | 256 | 224 |
Endosome | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 2 | 0 | 0 | 2 | 0 |
Golgi | 0 | 0 | 9 | 2 | 0 | 0 | 0 | 0 | – | 1 | 1 | 0 | 3 | 4 | 3 | 1 | 1 | 6 | 2 | 1 | 1 | 1 | 2 | 1 |
Mitochondria | 0 | 2 | 0 | 0 | 0 | 1 | 2 | 1 | – | 3 | 3 | 2 | 0 | 0 | 0 | 4 | 2 | 2 | 0 | 11 | 2 | 2 | 2 | 1 |
Nucleus | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | – | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 4 | – | 1 | 0 | 1 | 1 | 0 | 2 | 0 | 0 | 3 | 6 | 17 | 3 | 1 | 2 | 4 |
Unique Cell Count | 529 | 294 | 357 | 300 | 293 | 328 | 447 | 474 | 391 | 319 | 420 | 540 | 541 | 543 | 287 | 155 | 180 | 567 | 398 | 368 | 245 | 333 | 308 | |
Labelled Cell Count | 636 | 365 | 458 | 412 | 381 | 418 | 569 | 566 | 505 | 404 | 534 | 697 | 701 | 729 | 342 | 188 | 204 | 567 | 398 | 368 | 245 | 333 | 308 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 29.3 | 28.0 | 23.1 | 23.8 | 21.8 | 17.6 | 17.7 | 17.1 | – | 16.5 | 15.8 | 16.3 | 23.8 | 23.8 | 22.3 | 17.1 | 19.6 | 21.9 | 26.5 | 28.3 | 29.9 |
Std Deviation (1e-4) | 5.0 | 5.3 | 4.3 | 4.3 | 4.2 | 3.6 | 3.5 | 3.3 | – | 3.3 | 3.3 | 3.1 | 4.6 | 4.6 | 4.4 | 3.7 | 4.6 | 5.2 | 6.5 | 6.7 | 8.1 |
Intensity Change (Log2) | – | – | – | 0.04 | -0.08 | -0.39 | -0.38 | -0.44 | – | -0.49 | -0.55 | -0.51 | 0.04 | 0.04 | -0.05 | -0.43 | -0.24 | -0.08 | 0.2 | 0.29 | 0.37 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 1.4 | 1.6 | -1.9 | 0.2 | -3.1 | – | 0.8 | 0.6 | -0.4 | -1.7 | -1.6 | -2.5 | 0.3 | -1.8 | -1.7 |
Bud | 1.8 | -0.1 | -0.3 | 1.8 | 1.8 | – | 0.8 | 2.3 | 0 | -2.1 | -2.1 | 0 | -1.6 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 4.2 | 0.9 | -1.2 | -2.6 | -4.4 | – | -1.8 | -1.5 | -1.3 | 11.1 | 11.1 | 11.7 | 4.5 | 4.5 | 4.1 |
Cytoplasm | 0.6 | 2.7 | 8.3 | 8.5 | 10.7 | – | 10.4 | 9.7 | 9.3 | -1.8 | -1.8 | -2.1 | 12.2 | 10.6 | 9.9 |
Endoplasmic Reticulum | -3.4 | -2.9 | -4.7 | -6.1 | -7.9 | – | -8.4 | -9.2 | -7.0 | -7.6 | -7.5 | -5.9 | -18.0 | -13.8 | -15.3 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | -1.8 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | -2.2 | 0 | 0 | 0 | 0.5 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 15.621 | 20.2328 | 18.8911 | 15.5968 | 17.3825 | 19.448 | 19.891 | 22.6832 | 23.6287 | 19.8322 | 19.8023 | 21.5955 | 16.9916 | 15.4957 | 16.5998 | 17.2505 | 18.3549 | 16.8057 |
Actin | 0.0257 | 0.0015 | 0.0027 | 0.021 | 0.0184 | 0.0036 | 0.0391 | 0.0007 | 0.0132 | 0.0006 | 0.0285 | 0.0002 | 0.0506 | 0.0346 | 0.0333 | 0.0329 | 0.091 | 0.0528 |
Bud | 0.0005 | 0.0002 | 0.0002 | 0.0007 | 0.0004 | 0.0001 | 0.0026 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0255 | 0.0462 | 0.0645 | 0.012 | 0.0229 | 0.0069 |
Bud Neck | 0.0012 | 0.0022 | 0.0014 | 0.007 | 0.0211 | 0.0107 | 0.0014 | 0.0018 | 0.0025 | 0.0004 | 0.0038 | 0.0009 | 0.0212 | 0.0039 | 0.0062 | 0.0139 | 0.0027 | 0.156 |
Bud Periphery | 0.0009 | 0.0007 | 0.0007 | 0.0027 | 0.0011 | 0.0003 | 0.0054 | 0.0003 | 0.0008 | 0.0001 | 0.0008 | 0.0001 | 0.0593 | 0.0833 | 0.124 | 0.0549 | 0.0986 | 0.0361 |
Bud Site | 0.0041 | 0.0045 | 0.0008 | 0.0021 | 0.0024 | 0.0005 | 0.0027 | 0.0011 | 0.0004 | 0 | 0.0006 | 0 | 0.0945 | 0.1416 | 0.0379 | 0.012 | 0.0025 | 0.0053 |
Cell Periphery | 0.011 | 0.0381 | 0.0282 | 0.0156 | 0.0313 | 0.0049 | 0.0136 | 0.0227 | 0.0172 | 0.0062 | 0.0227 | 0.004 | 0.0312 | 0.0419 | 0.0358 | 0.0123 | 0.0198 | 0.0223 |
Cytoplasm | 0.0492 | 0.0812 | 0.0528 | 0.0398 | 0.0873 | 0.0377 | 0.0174 | 0.039 | 0.0289 | 0.0032 | 0.0157 | 0.0141 | 0.0416 | 0.0281 | 0.0197 | 0.0369 | 0.0186 | 0.0332 |
Cytoplasmic Foci | 0.0232 | 0.0021 | 0.0023 | 0.0056 | 0.0027 | 0.002 | 0.014 | 0.0004 | 0.0009 | 0.0002 | 0.0197 | 0.0003 | 0.015 | 0.0056 | 0.0048 | 0.0126 | 0.0117 | 0.0098 |
Eisosomes | 0.0004 | 0.0004 | 0.0002 | 0.0008 | 0.0004 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0.0006 | 0.0002 | 0 | 0.0031 | 0.0017 | 0.001 | 0.0006 | 0.003 | 0.0009 |
Endoplasmic Reticulum | 0.7137 | 0.8081 | 0.8491 | 0.7968 | 0.7581 | 0.8947 | 0.8106 | 0.9077 | 0.9119 | 0.9696 | 0.8152 | 0.9712 | 0.5437 | 0.4886 | 0.5632 | 0.6624 | 0.6442 | 0.5958 |
Endosome | 0.0431 | 0.0015 | 0.012 | 0.0117 | 0.0168 | 0.0096 | 0.0052 | 0.0007 | 0.0013 | 0.0032 | 0.0322 | 0.0008 | 0.0041 | 0.0031 | 0.0016 | 0.0054 | 0.0048 | 0.0046 |
Golgi | 0.0179 | 0.0018 | 0.0063 | 0.0099 | 0.0145 | 0.0035 | 0.0041 | 0.0011 | 0.0035 | 0.005 | 0.024 | 0.0005 | 0.0051 | 0.0021 | 0.0014 | 0.0029 | 0.0089 | 0.0039 |
Lipid Particles | 0.0438 | 0.0341 | 0.0195 | 0.0242 | 0.0171 | 0.0049 | 0.013 | 0.0136 | 0.0082 | 0.0026 | 0.0148 | 0.0026 | 0.0111 | 0.0185 | 0.0073 | 0.0086 | 0.0086 | 0.0085 |
Mitochondria | 0.0104 | 0.0006 | 0.0013 | 0.0443 | 0.0094 | 0.0018 | 0.0016 | 0 | 0.0001 | 0.0007 | 0.0021 | 0 | 0.0138 | 0.017 | 0.0115 | 0.0013 | 0.0034 | 0.0047 |
None | 0.0058 | 0.0023 | 0.003 | 0.0007 | 0.0003 | 0.0011 | 0.0052 | 0.0001 | 0.0001 | 0.0003 | 0.0008 | 0 | 0.0459 | 0.0464 | 0.0581 | 0.0601 | 0.0352 | 0.0358 |
Nuclear Periphery | 0.0181 | 0.0067 | 0.0073 | 0.0063 | 0.0053 | 0.0106 | 0.0207 | 0.0058 | 0.0057 | 0.0047 | 0.008 | 0.0027 | 0.0076 | 0.013 | 0.0119 | 0.0287 | 0.0092 | 0.0067 |
Nucleolus | 0.0006 | 0.0003 | 0.0002 | 0.0001 | 0.0003 | 0.001 | 0.0037 | 0 | 0 | 0 | 0.0001 | 0 | 0.0006 | 0.0012 | 0.0006 | 0.0007 | 0.0002 | 0.0004 |
Nucleus | 0.0045 | 0.0016 | 0.001 | 0.0008 | 0.0008 | 0.0041 | 0.0085 | 0.0003 | 0.0003 | 0.0001 | 0.0005 | 0.0001 | 0.0016 | 0.0063 | 0.0027 | 0.0036 | 0.0025 | 0.0017 |
Peroxisomes | 0.0054 | 0.0003 | 0.0001 | 0.0006 | 0.0011 | 0.0002 | 0.0029 | 0 | 0 | 0 | 0.0017 | 0 | 0.0034 | 0.0017 | 0.0002 | 0.0075 | 0.0016 | 0.0026 |
Punctate Nuclear | 0.0045 | 0.0004 | 0.0003 | 0.0003 | 0.0005 | 0.0021 | 0.0218 | 0.0001 | 0.0001 | 0 | 0.0015 | 0 | 0.0017 | 0.0013 | 0.0026 | 0.0016 | 0.0006 | 0.0013 |
Vacuole | 0.0139 | 0.0112 | 0.0099 | 0.0075 | 0.0098 | 0.0053 | 0.0053 | 0.0042 | 0.0046 | 0.0021 | 0.006 | 0.0023 | 0.0176 | 0.0128 | 0.0102 | 0.0226 | 0.0091 | 0.0095 |
Vacuole Periphery | 0.002 | 0.0002 | 0.0008 | 0.0014 | 0.001 | 0.0011 | 0.0008 | 0.0002 | 0.0003 | 0.0004 | 0.0007 | 0.0001 | 0.0017 | 0.0011 | 0.0015 | 0.0066 | 0.0008 | 0.0012 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 128.2 | 94.8439 | 92.2737 | 112.4493 | 102.787 | 108.9833 | 106.0189 | 101.9398 | 102.0127 | 130.3611 |
Translational Efficiency | 1.4857 | 1.4846 | 1.388 | 1.0827 | 1.5423 | 1.7734 | 1.2274 | 1.1354 | 1.1866 | 1.1984 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
129 | 418 | 1978 | 1392 | 604 | 1708 | 1084 | 561 | 733 | 2126 | 3062 | 1953 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1427.82 | 1944.08 | 1963.30 | 2159.85 | 1686.02 | 1793.03 | 1899.11 | 1916.90 | 1640.58 | 1822.73 | 1940.58 | 2090.06 |
Standard Deviation | 195.10 | 341.33 | 414.61 | 401.01 | 286.69 | 331.09 | 400.98 | 357.91 | 289.99 | 338.49 | 410.99 | 404.35 |
Intensity Change Log 2 | — | 0.445273 | 0.459467 | 0.597117 | — | 0.088778 | 0.171702 | 0.185153 | — | 0.263228 | 0.310807 | 0.388725 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.006291 | 0.000481 | 0.003638 | 0.001247 | 0.000092 | 0.001889 | 0.001017 | 0.003710 | 0.001183 | 0.001612 | 0.002710 | 0.001954 |
Bud Neck | 0.004814 | 0.000396 | 0.003890 | 0.001559 | 0.000335 | 0.000800 | 0.001875 | 0.004968 | 0.001123 | 0.000720 | 0.003177 | 0.002538 |
Bud Site | 0.017408 | 0.001704 | 0.015922 | 0.006225 | 0.001017 | 0.007925 | 0.005457 | 0.021448 | 0.003902 | 0.006702 | 0.012217 | 0.010597 |
Cell Periphery | 0.125604* | 0.051836 | 0.042471 | 0.302112* | 0.027834 | 0.025357 | 0.179522* | 0.284984* | 0.045040 | 0.030563 | 0.090990 | 0.297192* |
Cytoplasm | 0.056145 | 0.003508 | 0.048180 | 0.009917 | 0.001091 | 0.016681 | 0.014558 | 0.015439 | 0.010780 | 0.014091 | 0.036277 | 0.011503 |
Cytoplasmic Foci | 0.008692 | 0.002073 | 0.010425 | 0.000647 | 0.000905 | 0.005485 | 0.003253 | 0.003320 | 0.002275 | 0.004814 | 0.007886 | 0.001415 |
Eisosomes | 0.001445 | 0.000457 | 0.000852 | 0.001540 | 0.000175 | 0.000267 | 0.001609 | 0.001623 | 0.000399 | 0.000305 | 0.001120 | 0.001564 |
Endoplasmic Reticulum | 0.510531* | 0.901804* | 0.740672* | 0.479660* | 0.934541* | 0.888497* | 0.643594* | 0.347448* | 0.859920* | 0.891113* | 0.706305* | 0.441682* |
Endosome | 0.006432 | 0.003737 | 0.017248 | 0.003829 | 0.004413 | 0.008391 | 0.007312 | 0.010863 | 0.004769 | 0.007476 | 0.013731 | 0.005850 |
Golgi | 0.012369 | 0.004937 | 0.007413 | 0.008004 | 0.004094 | 0.004240 | 0.013228 | 0.010402 | 0.005550 | 0.004377 | 0.009472 | 0.008693 |
Lipid Particles | 0.090397 | 0.013028 | 0.053264 | 0.074435 | 0.008704 | 0.012959 | 0.053159 | 0.214729* | 0.023081 | 0.012973 | 0.053227 | 0.114734* |
Mitochondria | 0.000198 | 0.000142 | 0.001063 | 0.000600 | 0.000136 | 0.001482 | 0.000561 | 0.001425 | 0.000147 | 0.001219 | 0.000885 | 0.000837 |
Mitotic Spindle | 0.006715 | 0.000068 | 0.001071 | 0.001363 | 0.000056 | 0.000195 | 0.000816 | 0.003031 | 0.001228 | 0.000170 | 0.000981 | 0.001842 |
None | 0.000012 | 0.000057 | 0.002888 | 0.000285 | 0.000433 | 0.000859 | 0.000313 | 0.000888 | 0.000359 | 0.000701 | 0.001977 | 0.000458 |
Nuclear Periphery | 0.000508 | 0.000719 | 0.002552 | 0.014343 | 0.000494 | 0.000400 | 0.009117 | 0.004154 | 0.000496 | 0.000462 | 0.004876 | 0.011416 |
Nuclear Periphery Foci | 0.008435 | 0.000691 | 0.023163 | 0.003117 | 0.001666 | 0.001015 | 0.004239 | 0.004147 | 0.002857 | 0.000951 | 0.016463 | 0.003413 |
Nucleolus | 0.000283 | 0.000008 | 0.000087 | 0.000053 | 0.000007 | 0.000024 | 0.000035 | 0.000043 | 0.000056 | 0.000021 | 0.000068 | 0.000050 |
Nucleus | 0.001774 | 0.000094 | 0.001425 | 0.000675 | 0.000063 | 0.000240 | 0.000388 | 0.000502 | 0.000364 | 0.000212 | 0.001058 | 0.000625 |
Peroxisomes | 0.001288 | 0.002029 | 0.002599 | 0.005777 | 0.002544 | 0.001863 | 0.004967 | 0.002428 | 0.002323 | 0.001896 | 0.003437 | 0.004815 |
Vacuole | 0.139742 | 0.011295 | 0.020436 | 0.081918 | 0.009805 | 0.019917 | 0.051981 | 0.061452 | 0.032672 | 0.018222 | 0.031603 | 0.076039 |
Vacuole Periphery | 0.000918 | 0.000937 | 0.000741 | 0.002693 | 0.001595 | 0.001515 | 0.002998 | 0.002998 | 0.001476 | 0.001401 | 0.001540 | 0.002781 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 3.37 | 1.44 | 2.84 | -2.58 | 3.81 | -3.88 | -4.26 | -4.84 | -2.09 | -3.46 | -0.84 | -3.24 | -1.98 | -0.93 | 1.56 |
Bud Neck | 6.27 | 1.05 | 4.55 | -5.02 | 3.31 | -2.72 | -4.60 | -6.45 | -5.80 | -3.93 | 2.21 | -4.22 | -4.58 | -6.76 | 1.30 |
Bud Site | 3.84 | 0.48 | 2.68 | -4.17 | 5.00 | -5.49 | -5.42 | -5.89 | -3.70 | -4.49 | -2.02 | -6.07 | -4.47 | -2.57 | 0.98 |
Cell Periphery | 4.27 | 5.09 | -10.59 | -28.58* | -36.12* | 0.72 | -21.31 | -21.05 | -21.76 | -7.81 | 3.23 | -9.38 | -35.46 | -43.22* | -31.13* |
Cytoplasm | 6.39 | 0.97 | 5.60 | -4.20 | 12.30 | -10.38 | -5.99 | -6.10 | 0.36 | -0.34 | -1.50 | -9.62 | -0.33 | 1.52 | 10.72 |
Cytoplasmic Foci | 2.62 | -0.38 | 3.28 | 1.62 | 9.23 | -5.45 | -2.80 | -3.14 | 1.99 | -0.10 | -2.82 | -6.01 | 1.66 | 4.91 | 8.41 |
Eisosomes | 3.42 | 1.89 | -0.38 | -3.96 | -2.33 | -1.70 | -7.60 | -7.75 | -7.21 | -0.06 | 1.29 | -4.44 | -7.16 | -7.75 | -2.05 |
Endoplasmic Reticulum | -11.37 | -6.82 | 0.40 | 33.63* | 22.02 | 6.02 | 25.76 | 37.32* | 35.06* | 16.90 | -3.12 | 13.29 | 32.74 | 49.99* | 26.71 |
Endosome | 2.11 | -7.37 | 2.30 | -0.15 | 14.29 | -5.55 | -4.33 | -4.00 | -1.48 | -2.13 | -4.26 | -12.28 | -1.75 | 2.31 | 10.04 |
Golgi | 1.04 | 0.68 | 0.60 | -2.41 | -0.77 | -0.15 | -5.52 | -3.12 | -3.14 | 1.20 | 0.79 | -2.75 | -2.20 | -5.31 | 0.91 |
Lipid Particles | 5.24 | 2.46 | 1.06 | -13.53 | -4.29 | -1.94 | -10.70 | -17.61 | -17.32 | -13.27 | 3.02 | -7.74 | -16.83 | -22.02 | -12.06 |
Mitochondria | 0.58 | -3.69 | -4.01 | -6.30 | 1.99 | -3.96 | -5.23 | -2.56 | 0.11 | -1.70 | -3.91 | -4.95 | -4.52 | 1.22 | 0.26 |
Mitotic Spindle | 1.04 | 0.87 | 0.84 | -21.83* | -1.16 | -1.68 | -11.94 | -3.49 | -3.30 | -2.60 | 0.93 | 0.19 | -0.53 | -6.45 | -2.95 |
None | -1.95 | -3.42 | -3.40 | -2.75 | 3.08 | -0.87 | 0.31 | -0.50 | -0.03 | -0.69 | -0.86 | -2.60 | -0.26 | 0.67 | 2.55 |
Nuclear Periphery | -2.38 | -12.32 | -23.43 | -23.04 | -19.38 | 1.02 | -16.86 | -11.15 | -11.82 | 8.36 | 0.44 | -19.52 | -24.43 | -24.79 | -13.35 |
Nuclear Periphery Foci | 6.44 | -6.69 | 4.50 | -14.84 | 11.18 | 1.19 | -3.30 | -2.89 | -4.64 | 0.12 | 3.83 | -10.54 | -0.88 | -10.69 | 10.86 |
Nucleolus | 1.00 | 0.68 | 0.83 | -9.76 | 0.66 | -3.76 | -7.76 | -1.95 | -1.02 | -0.43 | 0.72 | -0.27 | 0.10 | -4.44 | 0.56 |
Nucleus | 3.08 | 0.70 | 2.09 | -10.96 | 4.71 | -6.37 | -8.82 | -6.55 | -3.90 | -1.59 | 1.62 | -4.68 | -2.09 | -9.39 | 4.10 |
Peroxisomes | -1.13 | -1.94 | -5.18 | -3.55 | -2.99 | 0.68 | -1.90 | 0.12 | -1.28 | 2.81 | 0.51 | -1.18 | -2.50 | -4.40 | -1.73 |
Vacuole | 7.83 | 7.31 | 3.97 | -22.36 | -20.70 | -4.03 | -13.36 | -11.69 | -9.40 | -1.96 | 3.96 | 0.77 | -8.69 | -21.26 | -17.07 |
Vacuole Periphery | -0.08 | 0.24 | -2.97 | -5.60 | -9.50 | 0.18 | -2.72 | -1.54 | -1.77 | 0.05 | 0.19 | -0.23 | -3.00 | -4.45 | -4.06 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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