Standard name
Human Ortholog
Description tRNA-specific 2-thiouridylase; responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human homolog TRMU is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF), and can complement yeast null mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0
Bud 0.07 0 0 0.05 0 0 0 0 0 0 0.06 0 0.05 0 0 0.05 0 0.1 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.07 0 0 0 0 0 0
Cytoplasm 0 0.2 0.12 0.05 0.05 0 0 0 0 0 0 0 0.24 0.3 0.37 0.1 0.19 0.13 0 0 0 0.06 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0 0.09 0.08 0.06 0.11 0 0 0 0 0 0 0 0.17 0.25 0.23 0.26 0.13 0.29 0.14 0.12 0.11 0.1 0.08 0.09
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0
Mitochondria 0.86 0.62 0.65 0.89 0.8 0.87 0.9 0.88 0.93 0.95 0.96 0.96 0.32 0.08 0.06 0.14 0.16 0.12 0.65 0.59 0.68 0.57 0.58 0.55
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0.07 0.23 0.14 0.15 0.09 0 0 0 0 0 0 0.09 0.07 0.1 0.23 0.22 0.2 0.06 0 0.09 0 0 0
SpindlePole 0.11 0.13 0.14 0.1 0.05 0.08 0.08 0.08 0 0.05 0 0 0.18 0.28 0.27 0.31 0.3 0.27 0.05 0.09 0 0.07 0.09 0.07
Vac/Vac Membrane 0.06 0.11 0.06 0 0.08 0.08 0 0 0 0 0 0 0.1 0.16 0.09 0.15 0.15 0.18 0 0.07 0 0.06 0.09 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 2 0 0 0 0 0 0 0 0 0 1 22 4 5 1 0 1 3 0 2 3 10 14
Bud 14 4 9 11 12 13 29 24 21 27 29 17 22 12 8 8 4 14 0 2 0 2 7 10
Bud Neck 0 0 0 0 0 0 0 1 1 0 0 0 6 6 6 0 1 0 1 2 1 4 10 17
Bud Site 1 1 2 3 0 6 23 17 6 13 10 10 1 0 2 2 3 3 – – – – – –
Cell Periphery 0 0 6 3 3 4 3 3 6 2 1 6 16 5 3 8 9 10 0 0 0 0 1 1
Cytoplasm 7 26 28 10 23 15 19 13 7 12 11 6 112 138 196 16 27 19 1 5 1 12 21 18
Endoplasmic Reticulum 6 1 2 1 2 1 2 1 3 3 1 7 1 3 10 3 9 4 1 0 0 0 3 4
Endosome 5 12 18 12 46 11 5 29 5 0 0 2 81 116 121 40 19 42 32 18 26 21 48 59
Golgi 0 0 1 2 0 0 0 0 0 0 0 0 3 3 6 7 7 9 2 0 6 2 5 4
Mitochondria 178 81 148 194 340 350 653 711 496 715 454 608 151 35 34 22 23 18 149 91 167 123 343 369
Nucleus 1 0 1 1 3 11 28 27 10 13 13 4 1 1 1 2 4 3 0 1 0 2 4 8
Nuclear Periphery 2 0 0 0 0 8 17 21 12 19 8 23 0 0 0 1 2 1 1 0 1 0 1 4
Nucleolus 1 1 2 0 4 7 26 15 14 23 8 11 0 1 1 3 2 3 1 1 0 0 3 5
Peroxisomes 7 9 52 30 64 38 23 34 6 19 2 5 41 33 50 36 32 30 13 2 22 3 15 29
SpindlePole 23 17 32 22 23 33 58 63 21 40 12 21 86 129 144 48 43 39 10 13 8 15 50 44
Vac/Vac Membrane 12 14 14 6 34 32 25 36 23 18 18 23 46 72 47 24 21 26 4 10 2 13 52 63
Unique Cell Count 206 130 226 218 424 404 726 808 531 749 473 631 472 464 526 156 144 147 229 155 246 215 590 673
Labelled Cell Count 259 168 315 295 554 529 911 995 631 904 567 744 589 558 634 221 206 222 229 155 246 215 590 673


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 4.3 4.7 4.4 4.6 4.1 4.0 4.0 3.9 3.8 3.5 3.7 5.7 5.6 5.5 6.1 6.7 6.4 5.5 5.6 6.1
Std Deviation (1e-4) 0.6 0.8 1.7 1.8 1.6 1.5 1.3 0.9 0.9 1.0 0.8 0.9 0.8 0.7 1.0 1.4 1.7 1.6 1.0 1.1 1.4
Intensity Change (Log2) – – – -0.09 -0.04 -0.19 -0.24 -0.23 -0.26 -0.3 -0.42 -0.34 0.27 0.25 0.23 0.38 0.5 0.44 0.22 0.25 0.37


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.9 2.7 3.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.8516 2.3013 2.3318 2.1557 2.0501 2.3044 0.0766 0.3533 0.142 0.0466 0.2373 0.1172 0.2884 0.4198 0.3066 0.3076 0.4112 0.1276
Actin 0.0481 0.045 0.0128 0.0189 0.0065 0.0343 0.0919 0.0254 0.0328 0.0334 0.0053 0.0219 0.0289 0.0174 0.0326 0.0223 0.0793 0.0105
Bud 0.0003 0.0018 0.0018 0.0023 0.0072 0.0007 0.0018 0.0019 0.0033 0.0071 0.0013 0.001 0.0009 0.0019 0.0061 0.0051 0.0219 0.001
Bud Neck 0.0004 0.0013 0.0063 0.0152 0.0004 0.0023 0.0059 0.0033 0.0008 0.0011 0.0002 0.0023 0.0043 0.0024 0.002 0.001 0.0029 0.0008
Bud Periphery 0.0007 0.003 0.0052 0.0135 0.026 0.002 0.003 0.0027 0.004 0.0273 0.0077 0.0024 0.0021 0.0026 0.0084 0.0053 0.0236 0.0017
Bud Site 0.0013 0.0401 0.022 0.0097 0.0059 0.0016 0.0283 0.0239 0.0096 0.0054 0.0003 0.0049 0.0056 0.0268 0.0172 0.0129 0.0091 0.0008
Cell Periphery 0.0003 0.0014 0.0029 0.0494 0.0007 0.0015 0.0013 0.001 0.0012 0.0061 0.0003 0.001 0.0008 0.0016 0.0019 0.0005 0.001 0.0006
Cytoplasm 0.0009 0.0024 0.0052 0.0052 0.0021 0.003 0.0021 0.0062 0.0115 0.0037 0.0009 0.0132 0.0056 0.0044 0.0038 0.002 0.0097 0.0015
Cytoplasmic Foci 0.0152 0.0264 0.041 0.0212 0.0174 0.0281 0.0298 0.0431 0.0422 0.0169 0.0082 0.0096 0.0325 0.0491 0.034 0.0238 0.0479 0.0289
Eisosomes 0.0007 0.0006 0.0021 0.0009 0.0008 0.0009 0.001 0.0004 0.0007 0.0004 0.0002 0.0009 0.0013 0.0004 0.0005 0.0003 0.0005 0.0004
Endoplasmic Reticulum 0.0018 0.0034 0.0018 0.0009 0.0005 0.0025 0.0028 0.0007 0.0019 0.0005 0.0018 0.0065 0.0044 0.0013 0.0014 0.0014 0.0017 0.0013
Endosome 0.071 0.0446 0.0398 0.0972 0.0884 0.0508 0.0593 0.0732 0.0619 0.0277 0.0874 0.0416 0.0975 0.0771 0.0872 0.0503 0.1003 0.05
Golgi 0.0399 0.0655 0.0301 0.0434 0.0349 0.0489 0.0563 0.0643 0.0775 0.0531 0.1115 0.0415 0.0464 0.0552 0.0352 0.0338 0.107 0.0373
Lipid Particles 0.0543 0.0269 0.0208 0.0147 0.0052 0.0211 0.0449 0.0317 0.0181 0.015 0.0375 0.0169 0.0946 0.0544 0.0178 0.0213 0.0183 0.0376
Mitochondria 0.6962 0.6372 0.7354 0.6417 0.7179 0.7138 0.6 0.6507 0.6631 0.7754 0.7263 0.8029 0.5586 0.5919 0.6874 0.7924 0.4826 0.769
None 0.0009 0.0006 0.0093 0.0019 0.0012 0.0277 0.0014 0.0006 0.0014 0.0007 0.0004 0.0013 0.0037 0.0093 0.0099 0.001 0.0067 0.0013
Nuclear Periphery 0.0109 0.0007 0.0005 0.0004 0.0003 0.0019 0.006 0.0004 0.0019 0.0003 0.0011 0.0049 0.0104 0.0015 0.0019 0.0005 0.0066 0.0023
Nucleolus 0.0004 0.0005 0.0008 0.0011 0.0004 0.0005 0.001 0.0025 0.0004 0.0003 0.0001 0.0005 0.0046 0.0036 0.0023 0.0009 0.0015 0.0008
Nucleus 0.0007 0.0005 0.0006 0.0011 0.0005 0.0004 0.0154 0.0011 0.0008 0.0005 0.0002 0.0009 0.0276 0.0013 0.0025 0.0006 0.0033 0.0006
Peroxisomes 0.0348 0.0891 0.0424 0.0306 0.065 0.0459 0.0345 0.0356 0.0575 0.018 0.0023 0.0148 0.0365 0.0676 0.0256 0.0144 0.0456 0.0398
Punctate Nuclear 0.0003 0.0002 0.0004 0.0003 0.0001 0.0013 0.0015 0.0003 0.0046 0.0002 0.0003 0.0017 0.0034 0.0005 0.0025 0.0002 0.006 0.0016
Vacuole 0.0086 0.0056 0.0133 0.0218 0.0109 0.006 0.0061 0.0212 0.0024 0.0034 0.0022 0.0036 0.0192 0.0166 0.0126 0.0042 0.0135 0.0045
Vacuole Periphery 0.0122 0.0031 0.0054 0.0083 0.0078 0.0048 0.0058 0.0099 0.0024 0.0033 0.0046 0.0057 0.0111 0.013 0.0072 0.0058 0.011 0.0077

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.5185 11.7988 8.1715 9.3281 12.5492 8.7942 11.9297 10.7357 14.4301 12.4903
Translational Efficiency 0.373 0.4175 0.5416 0.3759 0.4385 0.5068 0.5455 0.5361 0.2737 0.5042

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
965 581 2380 658 1442 288 218 478 2407 869 2598 1136

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 638.14 735.59 818.46 885.69 650.44 715.21 963.89 889.17 645.51 728.84 830.66 887.15
Standard Deviation 107.32 117.45 96.81 104.13 85.52 88.56 116.49 125.04 95.05 109.15 106.53 113.41
Intensity Change Log 2 — 0.205029 0.359039 0.472929 — 0.136951 0.567453 0.451043 — 0.171067 0.467999 0.461923

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001448 0.007239 0.003895 0.021849 0.001708 0.001920 0.015849 0.044838 0.001604 0.005476 0.004898 0.031523
Bud Neck 0.027148 0.016716 0.006952 0.010282 0.018293 0.012722 0.015725 0.014545 0.021843 0.015392 0.007688 0.012076
Bud Site 0.017450 0.035295 0.042473 0.080941 0.028842 0.017651 0.077398 0.153666* 0.024275 0.029447 0.045403 0.111542*
Cell Periphery 0.000880 0.000635 0.000368 0.000413 0.000357 0.000347 0.000433 0.002049 0.000567 0.000540 0.000373 0.001101
Cytoplasm 0.021017 0.009778 0.007820 0.015823 0.024740 0.008424 0.009481 0.023466 0.023247 0.009329 0.007960 0.019039
Cytoplasmic Foci 0.093026 0.086597 0.033880 0.097787 0.113908 0.142737 0.120642 0.060464 0.105537 0.105202 0.041160 0.082082
Eisosomes 0.000198 0.000177 0.000084 0.000151 0.000139 0.000180 0.000167 0.000249 0.000163 0.000178 0.000091 0.000192
Endoplasmic Reticulum 0.001897 0.000862 0.000948 0.002075 0.001560 0.000680 0.004277 0.002951 0.001695 0.000802 0.001227 0.002444
Endosome 0.087202 0.130170 0.084396 0.114726 0.083305 0.109983 0.136300 0.062438 0.084867 0.123480 0.088751 0.092724
Golgi 0.116334* 0.133453* 0.064970 0.092569 0.121488* 0.164170* 0.097517 0.076023 0.119422* 0.143633* 0.067701 0.085607
Lipid Particles 0.012273 0.005279 0.000723 0.001873 0.009700 0.006988 0.008008 0.000749 0.010731 0.005845 0.001335 0.001400
Mitochondria 0.488962* 0.404065* 0.610808* 0.252395* 0.489515* 0.395949* 0.171569* 0.279782* 0.489293* 0.401375* 0.573951* 0.263919*
Mitotic Spindle 0.007048 0.024898 0.044566 0.113993* 0.012885 0.012071 0.117104* 0.063517 0.010545 0.020647 0.050652 0.092754
None 0.002008 0.001040 0.000255 0.000173 0.000920 0.000316 0.000168 0.000211 0.001356 0.000800 0.000247 0.000189
Nuclear Periphery 0.000493 0.000537 0.000136 0.001621 0.000256 0.000084 0.000249 0.000074 0.000351 0.000387 0.000146 0.000970
Nuclear Periphery Foci 0.000398 0.000763 0.000205 0.001245 0.000370 0.000204 0.001202 0.000224 0.000381 0.000578 0.000289 0.000815
Nucleolus 0.002309 0.001557 0.000447 0.000353 0.001943 0.000559 0.000762 0.000109 0.002089 0.001226 0.000473 0.000250
Nucleus 0.005762 0.002964 0.001267 0.001956 0.004162 0.001394 0.005626 0.002448 0.004803 0.002444 0.001633 0.002163
Peroxisomes 0.085385 0.105008* 0.043237 0.117151* 0.049596 0.106179* 0.159899* 0.175973* 0.063944 0.105396* 0.053026 0.141902*
Vacuole 0.013409 0.021672 0.036256 0.055633 0.020051 0.009998 0.048263 0.027963 0.017388 0.017803 0.037263 0.043990
Vacuole Periphery 0.015352 0.011295 0.016315 0.016992 0.016263 0.007445 0.009363 0.008258 0.015898 0.010019 0.015732 0.013317

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.51 -4.48 -6.43 -4.10 -5.60 -0.31 -4.09 -8.47 -8.39 -4.78 -3.40 -5.58 -10.52 -8.56 -9.23
Bud Neck 2.65 6.15 4.88 2.46 -2.87 1.40 0.48 1.48 -0.48 0.55 2.55 8.03 5.00 1.44 -3.87
Bud Site -4.34 -9.41 -10.95 -6.90 -6.53 2.47 -4.96 -12.79 -13.26 -5.56 -1.59 -8.22 -16.03 -13.85 -11.74
Cell Periphery 1.06 2.29 2.09 2.83 -0.82 0.16 -1.04 -3.73 -3.73 -3.55 0.27 2.08 -2.47 -2.80 -3.73
Cytoplasm 3.89 5.39 0.94 -3.56 -5.71 6.41 5.58 0.27 -4.53 -3.93 7.18 9.23 1.19 -5.61 -7.34
Cytoplasmic Foci 1.06 14.90 -0.56 -1.48 -13.19 -2.91 -0.71 9.86 8.18 6.16 0.02 20.92 5.86 4.40 -11.38
Eisosomes 0.99 6.09 2.31 1.81 -6.71 -1.38 -1.80 -6.16 -2.13 -3.78 -1.01 7.94 -2.36 -0.95 -10.64
Endoplasmic Reticulum 1.70 1.60 -0.60 -3.19 -3.21 2.03 -2.74 -2.08 -3.53 1.16 2.49 1.23 -2.01 -4.68 -3.51
Endosome -5.19 0.50 -3.36 1.86 -4.21 -2.70 -4.28 3.48 4.59 5.83 -6.21 -0.97 -1.32 4.61 -0.56
Golgi -1.89 8.76 3.53 4.96 -4.39 -3.54 2.42 6.39 7.10 1.77 -3.46 12.43 6.88 8.40 -3.69
Lipid Particles 3.37 6.80 5.99 2.63 -3.86 1.47 0.50 7.26 4.69 2.91 3.60 9.04 9.13 4.68 -0.32
Mitochondria 4.67 -8.80 14.27 8.36 25.47* 4.10 16.02 11.68 4.54 -4.74 6.40 -8.13 18.89 9.30 26.09*
Mitotic Spindle -5.28 -14.68 -12.69 -10.00 -8.03 0.35 -8.20 -6.84 -6.84 3.73 -4.17 -15.54 -14.06 -11.68 -6.73
None 1.35 4.02 4.20 1.71 4.76 2.40 3.33 3.17 0.81 -1.79 1.31 5.03 5.28 1.78 3.92
Nuclear Periphery -0.09 2.31 -1.02 -0.91 -1.35 2.46 -0.14 2.67 0.11 3.06 -0.12 2.72 -0.97 -0.83 -1.29
Nuclear Periphery Foci -0.81 1.85 -2.11 -0.91 -2.58 1.93 -3.38 2.22 -0.22 3.92 -0.66 0.98 -1.88 -0.72 -2.15
Nucleolus 0.83 2.88 3.31 2.05 0.68 2.39 2.01 3.53 1.69 2.52 1.51 3.54 4.65 2.44 1.46
Nucleus 1.95 3.58 1.68 -0.12 -1.86 2.91 -0.53 0.93 -1.14 0.94 2.72 3.91 1.75 -0.60 -1.62
Peroxisomes -2.10 6.84 -3.11 -0.92 -8.83 -5.23 -6.59 -10.02 -4.29 -0.77 -6.13 2.71 -10.24 -3.79 -11.85
Vacuole -2.96 -10.59 -9.78 -7.02 -5.02 4.74 -4.94 -2.33 -4.78 2.98 -0.56 -10.39 -9.16 -7.82 -2.94
Vacuole Periphery 2.09 -0.54 -0.53 -2.58 -0.14 4.70 3.83 4.48 -0.38 0.48 4.60 0.12 1.87 -2.08 1.81
Standard name
Human Ortholog
Description tRNA-specific 2-thiouridylase; responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human homolog TRMU is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF), and can complement yeast null mutant
Localization
Cell Percentages mitochondrion (86%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Slm3

Slm3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Slm3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available