Standard name
Human Ortholog
Description Mitochondrial membrane transporter; mediates uptake of the essential cofactor thiamine pyrophosphate (ThPP) into mitochondria; expression appears to be regulated by carbon source; member of the mitochondrial carrier family
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
Bud 0.05 0 0 0 0 0 0 0 – 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Cytoplasm 0.52 0.74 0.58 0.66 0.65 0.55 0.38 0.67 – 0.22 0.31 0.31 0.89 0.94 0.96 0.75 0.69 0.71 0.15 0.22 0.16 0.08 0.07 0.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.09 0.08 0.09 0 0 0 0 0 0
Endosome 0 0.05 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0.11 0.05 0.05 0.09 0 0.06 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.55 0.15 0.35 0.34 0.34 0.59 0.79 0.41 – 0.84 0.85 0.81 0 0 0 0.08 0.07 0.07 0.64 0.43 0.62 0.61 0.61 0.58
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.05 0 0 0 0 0 – 0 0 0.05 0 0 0 0.06 0.05 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0.07 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 0.1 0 0.07 0 0.09 0.09 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 2 0 0 0 0 0 – 0 0 0 4 7 1 3 2 0 0 33 10 16 16 26
Bud 2 7 3 1 3 3 10 3 – 5 6 17 4 0 0 2 3 0 1 10 0 5 6 21
Bud Neck 0 4 4 0 1 5 1 0 – 2 1 0 8 10 3 3 4 1 0 12 1 12 17 20
Bud Site 0 0 0 0 0 0 1 2 – 2 1 4 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 10 6 0 0 1 0 2 – 5 1 2 5 6 3 6 4 7 0 8 3 2 5 6
Cytoplasm 23 369 104 61 114 132 102 135 – 59 66 94 477 620 642 198 150 87 8 150 38 45 37 65
Endoplasmic Reticulum 1 9 6 1 0 1 4 0 – 4 3 2 2 6 5 23 17 11 0 11 4 10 6 10
Endosome 0 23 0 1 1 0 0 0 – 0 0 0 16 10 9 14 24 6 2 60 7 31 23 24
Golgi 0 2 0 0 0 0 0 0 – 0 0 0 0 2 2 2 2 2 0 4 0 1 5 5
Mitochondria 24 74 63 31 59 141 210 84 – 224 181 249 2 3 0 20 15 9 38 297 148 346 315 390
Nucleus 0 1 0 0 1 2 2 2 – 0 2 4 0 3 0 2 1 2 0 0 0 1 1 0
Nuclear Periphery 0 0 0 1 0 0 0 0 – 2 2 0 0 0 1 1 1 0 0 2 1 1 0 3
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0
Peroxisomes 0 1 0 2 4 0 0 2 – 0 0 0 4 1 1 3 8 3 0 1 2 3 3 4
SpindlePole 0 9 9 3 6 6 1 9 – 10 3 14 8 1 3 15 11 4 0 17 5 22 18 18
Vac/Vac Membrane 2 34 3 0 7 4 1 2 – 4 1 1 26 11 12 8 8 12 1 49 9 53 44 64
Unique Cell Count 44 498 179 92 175 240 267 203 267 214 308 538 661 666 264 217 123 59 689 241 571 514 675
Labelled Cell Count 52 545 200 101 196 295 332 241 317 267 387 556 680 682 300 250 144 59 689 241 571 514 675


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.9 4.2 3.5 3.8 3.3 3.1 2.6 3.9 – 2.6 2.6 2.6 5.3 5.2 5.2 5.5 5.4 5.9 5.6 5.7 5.9
Std Deviation (1e-4) 2.4 0.9 0.6 1.5 1.2 0.9 1.2 11.6 – 0.3 0.5 0.4 0.8 0.9 1.0 1.3 1.3 1.9 1.1 1.1 1.4
Intensity Change (Log2) – – – 0.14 -0.06 -0.14 -0.39 0.18 – -0.43 -0.43 -0.41 0.62 0.57 0.58 0.66 0.63 0.77 0.68 0.71 0.77


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – 0
Bud Neck – – – – – – – – – – – – – – 0
Bud Site – – – – – – – – – – – – – – 0
Cell Periphery – – – – – – – – – – – – – – 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – 0
Endosome – – – – – – – – – – – – – – 0
Golgi – – – – – – – – – – – – – – 0
Mitochondria – – – – – – – – – – – – – – 0
Nuclear Periphery – – – – – – – – – – – – – – 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – 0
Peroxisomes – – – – – – – – – – – – – – 0
SpindlePole – – – – – – – – – – – – – – 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – 0
Cytoplasm – – – – – – – – – – – – – – 0
Nucleus – – – – – – – – – – – – – – 0
Vacuole – – – – – – – – – – – – – – 3.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.2944 0.343 0.2749 -0.3705 -0.1397 0.3272 2.992 2.8328 3.3177 2.8423 2.7971 3.3067 0.8155 1.1334 1.2444 0.8255 0.9296 1.1071
Actin 0.0024 0.004 0.0063 0.0038 0.0029 0.0039 0.0294 0.0099 0.0207 0.007 0.0232 0.0093 0.002 0.0057 0.0045 0.0041 0.0058 0.0024
Bud 0.0016 0.0077 0.0026 0.0032 0.0086 0.0017 0.0024 0.0047 0.0061 0.0056 0.0069 0.0038 0.0015 0.0033 0.0046 0.0034 0.0026 0.0015
Bud Neck 0.0069 0.0019 0.0024 0.0247 0.005 0.0096 0.0063 0.0039 0.0043 0.0042 0.0148 0.0135 0.0026 0.0019 0.0045 0.0013 0.002 0.008
Bud Periphery 0.004 0.008 0.0109 0.01 0.0285 0.0025 0.0059 0.008 0.0135 0.0174 0.0111 0.0072 0.0048 0.0057 0.0108 0.0097 0.0078 0.0043
Bud Site 0.0079 0.0327 0.0101 0.0096 0.0201 0.0023 0.0098 0.0571 0.016 0.0048 0.0163 0.0032 0.0092 0.0278 0.0207 0.0028 0.0025 0.002
Cell Periphery 0.0017 0.0025 0.0017 0.0014 0.0011 0.0007 0.0024 0.0018 0.0037 0.0015 0.001 0.0013 0.0013 0.0018 0.0024 0.0007 0.0006 0.0008
Cytoplasm 0.0645 0.1198 0.0812 0.0651 0.0551 0.115 0.0873 0.1341 0.0807 0.0809 0.1079 0.1267 0.1132 0.1442 0.1362 0.0901 0.0963 0.1208
Cytoplasmic Foci 0.1327 0.1145 0.1398 0.1234 0.1452 0.1439 0.1353 0.1809 0.1264 0.1517 0.1929 0.1743 0.1234 0.1231 0.1215 0.1273 0.1823 0.159
Eisosomes 0.0011 0.0009 0.0015 0.0008 0.0007 0.0003 0.0013 0.0005 0.0015 0.0009 0.0004 0.0004 0.0006 0.0006 0.0007 0.0003 0.0003 0.0002
Endoplasmic Reticulum 0.0036 0.0096 0.0064 0.0021 0.0043 0.0043 0.0067 0.003 0.0039 0.0063 0.0035 0.0052 0.0095 0.008 0.0069 0.0063 0.0028 0.006
Endosome 0.1456 0.1655 0.1462 0.1099 0.1525 0.1795 0.1643 0.1769 0.1399 0.1752 0.2333 0.1498 0.1859 0.219 0.163 0.1983 0.2255 0.1922
Golgi 0.0293 0.0283 0.0289 0.0362 0.0276 0.0347 0.0526 0.0536 0.0428 0.036 0.0521 0.0417 0.0343 0.0431 0.0354 0.0416 0.0425 0.037
Lipid Particles 0.0513 0.0223 0.0206 0.0091 0.0314 0.0307 0.0353 0.0065 0.0099 0.0162 0.0147 0.0186 0.0366 0.0137 0.0157 0.0301 0.0189 0.019
Mitochondria 0.1689 0.1643 0.1837 0.2449 0.1504 0.1634 0.1576 0.1631 0.2343 0.251 0.1153 0.1546 0.1731 0.1903 0.2033 0.2328 0.2032 0.1985
None 0.2992 0.24 0.2794 0.2607 0.2896 0.2255 0.2332 0.1439 0.2279 0.1264 0.1369 0.208 0.2401 0.1552 0.1974 0.1786 0.1263 0.176
Nuclear Periphery 0.0015 0.0048 0.0032 0.0013 0.0012 0.0018 0.0054 0.0007 0.0038 0.0028 0.0012 0.0018 0.0049 0.0032 0.0027 0.002 0.0017 0.0019
Nucleolus 0.0052 0.0029 0.0036 0.002 0.0021 0.0012 0.0024 0.002 0.0015 0.0013 0.0013 0.0013 0.0019 0.002 0.0028 0.0023 0.0011 0.0016
Nucleus 0.0033 0.0056 0.0044 0.0032 0.0022 0.0024 0.0038 0.0024 0.0032 0.0025 0.0025 0.0029 0.0033 0.0043 0.0049 0.0043 0.0017 0.003
Peroxisomes 0.0419 0.0334 0.0402 0.0699 0.0516 0.0536 0.0332 0.0279 0.0371 0.0777 0.0391 0.0572 0.026 0.0152 0.0264 0.023 0.0513 0.0447
Punctate Nuclear 0.008 0.0041 0.0083 0.003 0.0025 0.0034 0.0075 0.0032 0.0043 0.0018 0.0016 0.0028 0.0026 0.0028 0.0041 0.0022 0.0013 0.0039
Vacuole 0.0157 0.0212 0.0136 0.0116 0.0128 0.0145 0.0139 0.0125 0.0143 0.0196 0.0198 0.012 0.018 0.0232 0.0254 0.0312 0.0167 0.0127
Vacuole Periphery 0.0035 0.0061 0.0047 0.0041 0.0046 0.0049 0.004 0.0034 0.0041 0.0091 0.0041 0.0046 0.005 0.0059 0.0062 0.0075 0.0069 0.0044

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.2543 10.4378 2.3236 7.2906 1.5139 4.6679 8.1791 4.9861 8.7377 8.0079
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1741 1657 104 1961 2206 1804 2242 1967 3947 3461 2346 3928

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 620.86 712.75 858.13 812.25 680.85 683.11 798.83 836.07 654.39 697.30 801.46 824.18
Standard Deviation 75.04 97.22 107.12 107.95 91.65 91.13 108.69 116.38 89.81 95.25 109.30 112.88
Intensity Change Log 2 — 0.199128 0.466928 0.387656 — 0.004781 0.230552 0.296287 — 0.100746 0.348131 0.340588

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000616 0.002581 0.007445 0.003012 0.000500 0.001112 0.002604 0.003253 0.000551 0.001815 0.002818 0.003132
Bud Neck 0.044097 0.036122 0.032646 0.044741 0.040177 0.033580 0.067494 0.050875 0.041906 0.034797 0.065949 0.047813
Bud Site 0.015715 0.035165 0.035006 0.062726 0.020630 0.018579 0.089856 0.067264 0.018462 0.026520 0.087425 0.064998
Cell Periphery 0.000230 0.000259 0.000493 0.000224 0.000157 0.000543 0.000156 0.000210 0.000189 0.000407 0.000171 0.000217
Cytoplasm 0.117764* 0.055462 0.151122* 0.357018* 0.108308* 0.047535 0.234525* 0.320751* 0.112479* 0.051330 0.230827* 0.338857*
Cytoplasmic Foci 0.314238* 0.219164* 0.188322* 0.084883 0.326120* 0.190555* 0.133292 0.107642 0.320879* 0.204252* 0.135731 0.096280
Eisosomes 0.000329 0.000256 0.000297 0.000107 0.000195 0.000209 0.000103 0.000135 0.000254 0.000231 0.000112 0.000121
Endoplasmic Reticulum 0.002673 0.001076 0.015450 0.003358 0.001802 0.000943 0.002850 0.002452 0.002186 0.001007 0.003408 0.002904
Endosome 0.071858 0.082596 0.050617 0.016687 0.083415 0.102282 0.038602 0.026036 0.078317 0.092857 0.039135 0.021369
Golgi 0.043726 0.100500 0.007546 0.003111 0.052398 0.090867 0.015855 0.010951 0.048573 0.095479 0.015486 0.007037
Lipid Particles 0.034195 0.009434 0.021139 0.004356 0.016506 0.016677 0.003305 0.005522 0.024309 0.013209 0.004095 0.004940
Mitochondria 0.211174* 0.319292* 0.008360 0.016334 0.206019* 0.361391* 0.066897 0.028358 0.208293* 0.341235* 0.064302 0.022355
Mitotic Spindle 0.003519 0.013394 0.019925 0.054232 0.007975 0.012171 0.073646 0.044843 0.006009 0.012756 0.071264 0.049530
None 0.001594 0.000779 0.001748 0.003217 0.001149 0.001125 0.001351 0.002288 0.001345 0.000959 0.001369 0.002752
Nuclear Periphery 0.001257 0.000208 0.003125 0.002716 0.000463 0.000395 0.000953 0.002496 0.000813 0.000305 0.001050 0.002606
Nuclear Periphery Foci 0.000449 0.000237 0.006366 0.001242 0.000355 0.000428 0.000632 0.000824 0.000396 0.000337 0.000887 0.001033
Nucleolus 0.002454 0.001389 0.007077 0.002730 0.001826 0.002165 0.002377 0.002946 0.002103 0.001794 0.002586 0.002838
Nucleus 0.033118 0.009591 0.211863* 0.146034* 0.020012 0.009201 0.068032 0.124112 0.025793 0.009388 0.074408 0.135056*
Peroxisomes 0.032598 0.053866 0.055148 0.028238 0.029852 0.047576 0.049599 0.038696 0.031063 0.050588 0.049845 0.033475
Vacuole 0.054364 0.046208 0.171241 0.161741 0.067988 0.046916 0.142866 0.155624 0.061978 0.046577 0.144124 0.158678
Vacuole Periphery 0.014033 0.012420 0.005064 0.003296 0.014152 0.015750 0.005007 0.004724 0.014100 0.014156 0.005009 0.004011

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.58 -3.87 -5.45 0.58 2.92 -2.80 -9.73 -7.06 -4.29 -0.51 -4.43 -9.76 -9.05 -2.00 0.92
Bud Neck 2.35 1.69 2.24 -0.36 -0.94 2.30 -8.76 -2.50 -4.55 6.34 3.25 -8.37 -0.12 -3.61 8.64
Bud Site -8.72 -2.88 -10.19 -3.09 -1.97 1.24 -18.80 -11.55 -11.87 6.15 -5.89 -19.92 -15.27 -10.65 8.18
Cell Periphery -0.87 -4.65 2.64 2.72 5.66 -2.61 0.34 -0.90 2.46 -2.43 -2.76 1.34 0.58 2.95 -1.11
Cytoplasm 12.42 -1.68 -29.48 -41.69 -10.76 14.33 -20.44 -29.35 -40.20 -9.44 18.85 -20.90 -41.86 -57.94 -13.55
Cytoplasmic Foci 13.41 7.78 41.14 27.43 5.46 20.73 34.47 39.19 16.60 5.71 24.22 40.28 57.07 30.87 11.14
Eisosomes 1.59 0.45 5.83 6.90 7.50 -0.79 7.19 4.92 5.73 -3.62 0.98 7.42 7.41 8.98 -0.21
Endoplasmic Reticulum 4.60 -6.10 -2.35 -10.87 5.79 3.66 -4.31 -2.45 -5.70 1.65 5.87 -5.05 -3.69 -11.24 2.44
Endosome -2.71 3.27 20.94 22.63 4.73 -4.39 15.13 20.60 22.52 6.60 -4.92 16.88 29.46 31.76 10.70
Golgi -13.68 11.65 20.56 27.10 1.75 -10.04 16.82 19.02 24.23 3.67 -16.61 20.01 27.91 36.37 7.36
Lipid Particles 11.84 3.80 16.04 5.49 6.57 -0.17 13.43 10.68 8.09 -3.89 8.49 19.77 19.23 9.80 -1.36
Mitochondria -11.07 28.53 31.54* 40.77* -1.16 -16.81 23.64 31.70* 44.29* 11.10 -19.81 30.52 44.92* 60.15* 13.94
Mitotic Spindle -6.89 -1.82 -14.09 -10.51 -3.47 -2.63 -18.10 -11.18 -9.17 6.46 -6.17 -18.99 -17.78 -13.94 5.64
None 2.85 -0.28 -2.79 -5.07 -1.83 0.17 -0.58 -2.45 -2.56 -2.32 1.92 0.11 -3.80 -5.42 -4.11
Nuclear Periphery 4.34 -3.13 -6.07 -13.32 0.52 0.69 -7.03 -4.93 -5.09 -3.39 4.27 -3.44 -7.85 -10.50 -6.47
Nuclear Periphery Foci 1.84 -6.12 -6.22 -8.92 5.11 -0.70 -4.63 -5.49 -4.26 -1.98 0.77 -6.54 -8.34 -9.48 -1.91
Nucleolus 2.91 -2.60 -0.50 -3.40 2.49 -0.75 -2.09 -2.28 -1.37 -0.39 0.99 -2.27 -2.14 -3.05 0.39
Nucleus 10.77 -8.12 -27.67 -34.41 2.43 6.97 -18.65 -26.92 -29.63 -11.41 12.61 -18.44 -38.93 -45.24 -15.25
Peroxisomes -6.95 -1.90 5.30 11.15 3.34 -6.74 -6.41 -1.03 4.91 4.56 -9.76 -6.47 2.75 11.22 7.99
Vacuole 2.36 -7.33 -29.01 -30.67 -1.44 6.27 -19.98 -22.43 -26.44 -4.79 6.43 -23.63 -36.17 -40.35 -8.51
Vacuole Periphery 1.60 7.09 15.00 14.50 2.05 -1.64 14.51 14.10 13.74 1.54 -0.10 17.85 20.87 19.78 4.43
Standard name
Human Ortholog
Description Mitochondrial membrane transporter; mediates uptake of the essential cofactor thiamine pyrophosphate (ThPP) into mitochondria; expression appears to be regulated by carbon source; member of the mitochondrial carrier family
Localization
Cell Percentages mitochondrion (57%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Tpc1

Tpc1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tpc1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available