Standard name | |
---|---|
Human Ortholog | |
Description | 3-ketosphinganine reductase; catalyzes the second step in phytosphingosine synthesis; essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine; localized to lipid droplets; member of short chain dehydrogenase/reductase protein family |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 5 | 7 | 7 | 4 | 8 | 13 | 1 | 6 | 9 | 11 | 9 | 7 | 2 | 10 | 9 | – | 2 | 4 | 5 | 5 | 12 | 3 | 7 | 12 |
Bud | 0 | 0 | 3 | 2 | 33 | 22 | 22 | 28 | 26 | 19 | 21 | 31 | 1 | 1 | 2 | – | 1 | 1 | 0 | 0 | 0 | 0 | 3 | 1 |
Bud Neck | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | – | 0 | 1 | 1 | 2 | 0 | 1 | 4 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 5 | 6 | 1 | 6 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 7 | 3 | 3 | 29 | 10 | 21 | 10 | 3 | 1 | 5 | 2 | 4 | 10 | 13 | – | 0 | 2 | 0 | 0 | 0 | 1 | 5 | 6 |
Cytoplasm | 14 | 63 | 67 | 115 | 330 | 223 | 275 | 169 | 100 | 98 | 57 | 78 | 17 | 131 | 160 | – | 7 | 25 | 5 | 28 | 8 | 4 | 30 | 30 |
Endoplasmic Reticulum | 46 | 67 | 66 | 83 | 87 | 114 | 134 | 73 | 84 | 69 | 72 | 51 | 18 | 57 | 85 | – | 1 | 0 | 60 | 87 | 137 | 29 | 107 | 91 |
Endosome | 0 | 3 | 7 | 14 | 26 | 8 | 34 | 54 | 26 | 68 | 12 | 23 | 0 | 2 | 6 | – | 2 | 2 | 2 | 4 | 3 | 1 | 2 | 3 |
Golgi | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | – | 1 | 0 | 0 | 4 | 1 | 0 | 1 | 1 |
Mitochondria | 6 | 3 | 22 | 47 | 198 | 219 | 466 | 334 | 415 | 567 | 447 | 700 | 0 | 0 | 0 | – | 3 | 2 | 5 | 9 | 19 | 10 | 34 | 19 |
Nucleus | 0 | 1 | 0 | 2 | 0 | 1 | 8 | 6 | 4 | 9 | 4 | 5 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 2 | 6 | 15 | 4 | 9 | 6 | 15 | 10 | 0 | 0 | 0 | – | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 3 | 6 | 20 | 8 | 42 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 2 | 3 | 4 | 2 | 2 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 16 | 6 | 21 | 1 | 13 | 0 | 1 | 6 | – | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 7 | 10 | 16 | 39 | 30 | 135 | 91 | 82 | 138 | 52 | 71 | 0 | 1 | 2 | – | 1 | 3 | 3 | 7 | 7 | 9 | 28 | 26 |
Unique Cell Count | 68 | 134 | 162 | 252 | 605 | 511 | 892 | 669 | 615 | 870 | 571 | 882 | 37 | 201 | 253 | 17 | 39 | 88 | 155 | 194 | 65 | 226 | 199 | |
Labelled Cell Count | 74 | 161 | 186 | 288 | 755 | 648 | 1117 | 796 | 778 | 1037 | 707 | 1042 | 42 | 215 | 284 | 19 | 42 | 88 | 155 | 194 | 65 | 226 | 199 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.5 | 7.1 | 5.5 | 5.7 | 5.4 | 5.2 | 4.9 | 5.5 | 5.4 | 5.3 | 5.6 | 5.5 | 6.6 | 6.6 | 6.6 | – | 7.6 | 6.8 | 7.9 | 7.2 | 7.8 |
Std Deviation (1e-4) | 0.8 | 1.3 | 1.2 | 1.4 | 1.4 | 1.4 | 1.2 | 1.5 | 1.2 | 1.4 | 1.2 | 1.2 | 0.6 | 1.1 | 1.0 | – | 2.4 | 1.4 | 1.1 | 1.2 | 1.5 |
Intensity Change (Log2) | – | – | – | 0.05 | -0.02 | -0.07 | -0.16 | -0.0 | -0.02 | -0.03 | 0.02 | -0.0 | 0.26 | 0.27 | 0.26 | – | 0.47 | 0.32 | 0.53 | 0.4 | 0.52 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.3 | -0.4 | – | 0 | 0 |
Bud | 0 | 1.9 | 1.4 | 0 | 1.4 | 1.4 | 0 | 1.2 | 1.1 | 0 | 0 | 0 | – | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Cell Periphery | 0 | 1.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.6 | 1.7 | – | 0 | 0 |
Cytoplasm | 0.9 | 3.0 | 0.5 | -2.6 | -4.1 | -6.9 | -9.6 | -9.4 | -11.0 | 0.5 | 4.5 | 4.4 | – | 0 | 2.6 |
Endoplasmic Reticulum | -1.6 | -7.5 | -4.6 | -7.7 | -9.1 | -7.8 | -11.4 | -8.1 | -13.0 | 0.9 | -2.5 | -1.5 | – | -2.8 | -4.9 |
Endosome | 0.6 | 0 | 0 | -0.3 | 1.6 | -0.1 | 1.6 | 0 | 0 | 0 | 0 | -1.1 | – | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Mitochondria | 1.4 | 4.8 | 6.8 | 9.1 | 8.4 | 12.3 | 12.2 | 15.1 | 16.7 | 0 | -5.4 | -6.0 | – | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.8 | 0 | 0 | 0 | – | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Vacuole | 0.1 | 0.1 | -0.1 | 3.0 | 2.6 | 2.5 | 3.2 | 1.2 | 0.8 | 0 | 0 | 0 | – | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.8342 | 3.6419 | 3.4452 | 3.5223 | 2.7821 | 3.6653 | 3.0929 | 3.5321 | 3.1172 | 2.7201 | 2.9836 | 3.1971 | 1.6235 | 2.0981 | 1.783 | 1.904 | 1.4279 | 1.927 |
Actin | 0.0144 | 0.0081 | 0.0123 | 0.0406 | 0.0149 | 0.0044 | 0.0086 | 0.0106 | 0.0219 | 0.0031 | 0.0116 | 0.0069 | 0.021 | 0.0155 | 0.0064 | 0.0019 | 0.0029 | 0.01 |
Bud | 0.002 | 0.0033 | 0.0018 | 0.0018 | 0.0055 | 0.0058 | 0.0001 | 0.0006 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0002 |
Bud Neck | 0.0044 | 0.0037 | 0.0094 | 0.024 | 0.0032 | 0.0101 | 0.0014 | 0.0025 | 0.0027 | 0.0008 | 0.0262 | 0.0032 | 0.0036 | 0.0005 | 0.0029 | 0.003 | 0.0013 | 0.0102 |
Bud Periphery | 0.0018 | 0.0017 | 0.0021 | 0.0024 | 0.005 | 0.0047 | 0.0003 | 0.001 | 0.0009 | 0.0004 | 0.0006 | 0.0003 | 0.0006 | 0.0002 | 0.0004 | 0.0004 | 0.0001 | 0.0005 |
Bud Site | 0.016 | 0.0521 | 0.0176 | 0.0076 | 0.0051 | 0.0054 | 0.0015 | 0.0421 | 0.0058 | 0.0004 | 0.003 | 0.0004 | 0.0032 | 0.0028 | 0.0052 | 0.0044 | 0.0005 | 0.0005 |
Cell Periphery | 0.0031 | 0.0037 | 0.0035 | 0.0018 | 0.0025 | 0.003 | 0.0107 | 0.0094 | 0.0124 | 0.0046 | 0.0063 | 0.0048 | 0.0081 | 0.0058 | 0.0056 | 0.0026 | 0.002 | 0.0044 |
Cytoplasm | 0.1483 | 0.1987 | 0.1926 | 0.1543 | 0.2545 | 0.2462 | 0.1781 | 0.2859 | 0.2185 | 0.1594 | 0.2095 | 0.2748 | 0.1573 | 0.2416 | 0.2034 | 0.1808 | 0.1798 | 0.1692 |
Cytoplasmic Foci | 0.0796 | 0.08 | 0.0681 | 0.0515 | 0.0895 | 0.0717 | 0.0617 | 0.0457 | 0.0479 | 0.0766 | 0.0612 | 0.0396 | 0.0669 | 0.0752 | 0.0549 | 0.0401 | 0.062 | 0.0511 |
Eisosomes | 0.0003 | 0.0002 | 0.0005 | 0.0003 | 0.0004 | 0.0002 | 0.0023 | 0.0004 | 0.0008 | 0.0002 | 0.0006 | 0.0002 | 0.0008 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.229 | 0.2623 | 0.2271 | 0.2965 | 0.1722 | 0.2363 | 0.2299 | 0.2591 | 0.3603 | 0.3906 | 0.3316 | 0.3326 | 0.2787 | 0.2172 | 0.32 | 0.5138 | 0.458 | 0.4089 |
Endosome | 0.1529 | 0.1442 | 0.1536 | 0.1251 | 0.1704 | 0.1333 | 0.063 | 0.0824 | 0.0756 | 0.1435 | 0.0946 | 0.0521 | 0.0875 | 0.0767 | 0.094 | 0.1103 | 0.1216 | 0.0863 |
Golgi | 0.0289 | 0.0186 | 0.0307 | 0.0421 | 0.0439 | 0.0176 | 0.0254 | 0.0215 | 0.0257 | 0.0358 | 0.026 | 0.0266 | 0.0409 | 0.0181 | 0.0362 | 0.0375 | 0.0449 | 0.0491 |
Lipid Particles | 0.1408 | 0.1293 | 0.13 | 0.1012 | 0.0756 | 0.1116 | 0.2883 | 0.1382 | 0.1694 | 0.0956 | 0.128 | 0.1921 | 0.2549 | 0.2994 | 0.2181 | 0.0658 | 0.1063 | 0.1663 |
Mitochondria | 0.016 | 0.0025 | 0.0205 | 0.0109 | 0.0277 | 0.0112 | 0.0036 | 0.0076 | 0.0029 | 0.0044 | 0.0034 | 0.0015 | 0.0093 | 0.0004 | 0.01 | 0.0055 | 0.0022 | 0.0035 |
None | 0.072 | 0.0262 | 0.0618 | 0.0301 | 0.0508 | 0.0799 | 0.0635 | 0.0679 | 0.022 | 0.0693 | 0.0812 | 0.0491 | 0.0316 | 0.0246 | 0.0226 | 0.0062 | 0.0014 | 0.0157 |
Nuclear Periphery | 0.0124 | 0.0138 | 0.016 | 0.0228 | 0.0088 | 0.0174 | 0.0068 | 0.0063 | 0.0078 | 0.0042 | 0.0049 | 0.0038 | 0.0099 | 0.0045 | 0.0067 | 0.0052 | 0.006 | 0.0087 |
Nucleolus | 0.0094 | 0.002 | 0.0024 | 0.0146 | 0.0239 | 0.0018 | 0.0003 | 0.0004 | 0.0007 | 0.0001 | 0.0002 | 0.0001 | 0.0012 | 0.0001 | 0.0001 | 0.0015 | 0.0001 | 0.0001 |
Nucleus | 0.0125 | 0.007 | 0.0067 | 0.0326 | 0.0082 | 0.0077 | 0.0007 | 0.0016 | 0.002 | 0.0006 | 0.0011 | 0.0006 | 0.0016 | 0.001 | 0.0008 | 0.0011 | 0.0009 | 0.0012 |
Peroxisomes | 0.0132 | 0.0049 | 0.0103 | 0.0091 | 0.0064 | 0.003 | 0.0245 | 0.0009 | 0.0018 | 0.0023 | 0.0017 | 0.0021 | 0.0075 | 0.0017 | 0.0019 | 0.0036 | 0.0018 | 0.0017 |
Punctate Nuclear | 0.0204 | 0.0166 | 0.0152 | 0.0154 | 0.0159 | 0.0116 | 0.0018 | 0.0027 | 0.0028 | 0.0008 | 0.0013 | 0.0008 | 0.0056 | 0.0018 | 0.0014 | 0.0031 | 0.0008 | 0.0016 |
Vacuole | 0.02 | 0.0193 | 0.0157 | 0.0117 | 0.0118 | 0.0149 | 0.0191 | 0.0123 | 0.0167 | 0.0061 | 0.0057 | 0.0074 | 0.0082 | 0.0122 | 0.0079 | 0.0107 | 0.0062 | 0.0095 |
Vacuole Periphery | 0.0025 | 0.0018 | 0.002 | 0.0034 | 0.004 | 0.0022 | 0.0084 | 0.0009 | 0.0011 | 0.0011 | 0.001 | 0.0009 | 0.0015 | 0.0005 | 0.001 | 0.0021 | 0.0011 | 0.0012 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 46.9778 | 40.971 | 28.8821 | 29.1835 | 47.5385 | 28.6291 | 39.3544 | 32.503 | 33.9326 | 40.083 |
Translational Efficiency | 0.9509 | 1.0546 | 1.0964 | 1.3594 | 1.0561 | 1.3803 | 1.0478 | 0.9862 | 0.9898 | 0.9014 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1477 | 488 | 311 | 1154 | 1077 | 452 | 2603 | 1087 | 2554 | 940 | 2914 | 2241 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 662.91 | 828.45 | 1140.33 | 1006.61 | 722.47 | 751.57 | 1040.53 | 998.70 | 688.03 | 791.48 | 1051.18 | 1002.77 |
Standard Deviation | 74.76 | 118.43 | 156.88 | 160.32 | 91.87 | 110.42 | 136.63 | 156.26 | 87.50 | 120.91 | 142.31 | 158.41 |
Intensity Change Log 2 | — | 0.321602 | 0.782566 | 0.602620 | — | 0.056970 | 0.526309 | 0.467114 | — | 0.189661 | 0.654615 | 0.533544 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001326 | 0.005214 | 0.005309 | 0.087769 | 0.002501 | 0.008630 | 0.034175 | 0.071881 | 0.001822 | 0.006857 | 0.031094 | 0.080062 |
Bud Neck | 0.011778 | 0.011826 | 0.005010 | 0.021823 | 0.008032 | 0.026536 | 0.013712 | 0.018840 | 0.010198 | 0.018899 | 0.012783 | 0.020376 |
Bud Site | 0.004657 | 0.015510 | 0.016325 | 0.082461 | 0.006339 | 0.019681 | 0.027800 | 0.063399 | 0.005366 | 0.017515 | 0.026575 | 0.073215 |
Cell Periphery | 0.000584 | 0.002075 | 0.005513 | 0.006376 | 0.001705 | 0.002456 | 0.003791 | 0.006086 | 0.001057 | 0.002258 | 0.003975 | 0.006235 |
Cytoplasm | 0.290819* | 0.203116* | 0.105214* | 0.103047* | 0.242089* | 0.201154* | 0.130457* | 0.128658* | 0.270270* | 0.202172* | 0.127763* | 0.115470* |
Cytoplasmic Foci | 0.223432* | 0.163118 | 0.048955 | 0.075764 | 0.184436* | 0.250629* | 0.031562 | 0.096355 | 0.206988* | 0.205198* | 0.033418 | 0.085752 |
Eisosomes | 0.001272 | 0.001727 | 0.001297 | 0.003823 | 0.002593 | 0.003232 | 0.004377 | 0.004755 | 0.001829 | 0.002451 | 0.004048 | 0.004275 |
Endoplasmic Reticulum | 0.099028 | 0.209229* | 0.475485* | 0.147457* | 0.159042* | 0.090504 | 0.341143* | 0.153709* | 0.124335* | 0.152140* | 0.355481* | 0.150489* |
Endosome | 0.023067 | 0.037621 | 0.034408 | 0.067955 | 0.030362 | 0.053660 | 0.036629 | 0.065885 | 0.026143 | 0.045334 | 0.036392 | 0.066951 |
Golgi | 0.044294 | 0.044279 | 0.003883 | 0.034389 | 0.086162 | 0.043321 | 0.028704 | 0.041640 | 0.061950 | 0.043818 | 0.026055 | 0.037906 |
Lipid Particles | 0.139862* | 0.144968* | 0.051318 | 0.204403* | 0.171445* | 0.093227 | 0.254057* | 0.196020* | 0.153180* | 0.120088* | 0.232420* | 0.200337* |
Mitochondria | 0.019787 | 0.007867 | 0.000321 | 0.004694 | 0.015941 | 0.029187 | 0.007815 | 0.003529 | 0.018165 | 0.018119 | 0.007015 | 0.004129 |
Mitotic Spindle | 0.001702 | 0.003752 | 0.000816 | 0.017400 | 0.000507 | 0.003478 | 0.003333 | 0.012864 | 0.001198 | 0.003621 | 0.003065 | 0.015200 |
None | 0.003823 | 0.002085 | 0.000824 | 0.000451 | 0.002283 | 0.000676 | 0.004489 | 0.000288 | 0.003174 | 0.001407 | 0.004098 | 0.000372 |
Nuclear Periphery | 0.001885 | 0.002110 | 0.006053 | 0.000533 | 0.001231 | 0.000528 | 0.000613 | 0.000407 | 0.001610 | 0.001349 | 0.001193 | 0.000472 |
Nuclear Periphery Foci | 0.003449 | 0.016072 | 0.110025 | 0.013516 | 0.004630 | 0.002888 | 0.011539 | 0.011662 | 0.003947 | 0.009733 | 0.022050 | 0.012616 |
Nucleolus | 0.001057 | 0.000841 | 0.000320 | 0.000272 | 0.000853 | 0.000924 | 0.000276 | 0.000292 | 0.000971 | 0.000881 | 0.000280 | 0.000282 |
Nucleus | 0.071809 | 0.052546 | 0.015051 | 0.020961 | 0.031511 | 0.053923 | 0.022352 | 0.024502 | 0.054816 | 0.053208 | 0.021573 | 0.022679 |
Peroxisomes | 0.012538 | 0.013905 | 0.001354 | 0.013931 | 0.016955 | 0.047833 | 0.015243 | 0.018745 | 0.014401 | 0.030219 | 0.013761 | 0.016266 |
Vacuole | 0.040574 | 0.058863 | 0.111306 | 0.090175 | 0.027847 | 0.063253 | 0.027120 | 0.079278 | 0.035207 | 0.060974 | 0.036105 | 0.084889 |
Vacuole Periphery | 0.003257 | 0.003276 | 0.001213 | 0.002800 | 0.003535 | 0.004281 | 0.000812 | 0.001204 | 0.003374 | 0.003759 | 0.000855 | 0.002026 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.89 | -2.32 | -15.68 | -14.40 | -14.06 | -2.78 | -15.16 | -13.71 | -11.34 | -6.35 | -3.98 | -15.77 | -20.84 | -18.26 | -10.76 |
Bud Neck | -0.05 | 5.04 | -5.45 | -4.56 | -11.00 | -5.11 | -4.67 | -6.48 | 2.08 | -2.93 | -4.27 | -2.51 | -8.06 | -0.51 | -5.74 |
Bud Site | -4.78 | -3.66 | -16.75 | -13.09 | -11.59 | -5.40 | -10.62 | -13.53 | -9.24 | -7.61 | -7.29 | -12.23 | -21.54 | -15.93 | -12.87 |
Cell Periphery | -3.45 | -4.33 | -8.38 | -4.86 | -0.16 | -1.03 | -6.17 | -5.89 | -3.58 | -2.77 | -2.99 | -11.01 | -10.31 | -5.92 | -3.39 |
Cytoplasm | 7.32 | 16.01 | 21.23 | 7.23 | -0.93 | 3.38 | 14.61 | 11.18 | 5.11 | -1.11 | 7.98 | 25.57 | 23.19 | 8.76 | 0.13 |
Cytoplasmic Foci | 6.56 | 24.76 | 25.19 | 10.70 | -3.81 | -6.21 | 29.20 | 13.87 | 15.12 | -14.27 | 0.13 | 45.88 | 27.81 | 17.99 | -16.45 |
Eisosomes | -1.41 | -0.01 | -5.45 | -3.48 | -2.57 | -0.58 | -5.08 | -3.29 | -1.11 | -0.09 | -1.16 | -8.21 | -6.23 | -2.50 | 0.37 |
Endoplasmic Reticulum | -7.58 | -16.99 | -3.58 | 5.14 | 15.19 | 5.62 | -17.54 | 1.74 | -4.14 | 19.33 | -2.64 | -29.12 | -1.57 | 1.50 | 26.09 |
Endosome | -4.70 | -3.54 | -10.91 | -5.32 | -5.85 | -4.74 | -2.86 | -7.90 | -1.25 | -6.37 | -6.71 | -6.15 | -13.45 | -4.28 | -8.87 |
Golgi | 0.14 | 18.80 | 5.17 | 3.75 | -12.04 | 8.42 | 15.05 | 10.96 | 1.10 | -3.55 | 5.98 | 16.45 | 11.22 | 3.36 | -4.24 |
Lipid Particles | -0.40 | 13.64 | -4.63 | -3.02 | -15.00 | 8.29 | -9.44 | -0.81 | -8.19 | 7.42 | 4.76 | -11.68 | -4.12 | -7.51 | 6.59 |
Mitochondria | 4.89 | 10.83 | 8.07 | 1.97 | -5.64 | -3.08 | 5.37 | 8.06 | 6.48 | 4.82 | 0.16 | 8.54 | 11.12 | 6.60 | 4.36 |
Mitotic Spindle | -1.00 | 1.14 | -6.10 | -4.44 | -6.68 | -1.50 | -4.50 | -5.36 | -3.31 | -3.95 | -1.68 | -3.03 | -8.04 | -5.50 | -6.68 |
None | 1.77 | 3.34 | 4.70 | 2.26 | 0.51 | 3.05 | -2.56 | 3.91 | 3.10 | 6.25 | 3.01 | -1.13 | 6.01 | 2.76 | 6.10 |
Nuclear Periphery | -0.53 | -4.66 | 5.31 | 3.81 | 6.17 | 4.04 | 3.41 | 4.36 | 0.44 | 2.45 | 1.11 | 2.22 | 6.91 | 3.80 | 5.35 |
Nuclear Periphery Foci | -5.04 | -11.04 | -8.41 | 0.77 | 9.88 | 2.07 | -7.82 | -5.33 | -6.42 | 0.31 | -4.28 | -13.25 | -9.85 | -1.92 | 6.00 |
Nucleolus | 0.87 | 3.42 | 3.88 | 2.99 | 0.24 | -0.61 | 2.68 | 2.36 | 3.41 | -0.29 | 0.19 | 5.04 | 4.49 | 4.53 | -0.28 |
Nucleus | 3.19 | 17.28 | 12.36 | 4.96 | -4.33 | -4.26 | 3.39 | 0.17 | 4.19 | -2.59 | -0.03 | 14.83 | 10.22 | 6.46 | -3.23 |
Peroxisomes | -0.78 | 12.89 | -0.07 | 0.70 | -10.89 | -6.59 | 1.42 | -0.36 | 6.35 | -1.61 | -6.34 | 0.86 | -0.59 | 5.95 | -1.33 |
Vacuole | -3.64 | -8.40 | -14.37 | -9.50 | -2.10 | -6.62 | -1.67 | -13.76 | -5.58 | -13.11 | -7.04 | -2.45 | -19.59 | -10.77 | -17.70 |
Vacuole Periphery | -0.00 | 3.14 | 1.11 | 0.96 | -1.27 | -1.09 | 8.21 | 6.74 | 5.69 | -1.57 | -0.80 | 10.83 | 3.79 | 3.74 | -2.48 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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