Standard name
Human Ortholog
Description Mitochondrial protein of unknown function; similar to Ups1p and Ups2p which are involved in regulation of mitochondrial cardiolipin and phosphatidylethanolamine levels; null is viable but interacts synthetically with ups1 and ups2 mutations; UPS3 has a paralog, UPS2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0.05 0 0 0 0 0.06 0.05 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.67 0.82 0.88 0.7 0.55 0.29 0.27 0.28 0.18 0.2 0.15 0.1 0.69 0.78 0.83 – 0.86 0.76 0.21 0.36 0.33 0.23 0.3 0.37
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0 0 – 0.05 0.06 0 0 0 0 0 0
Endosome 0 0 0 0.08 0.2 0 0.13 0.11 0 0.12 0 0.09 0 0.12 0.1 – 0 0.05 0.12 0.06 0 0.06 0.06 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.24 0.16 0 0.17 0.11 0.71 0.41 0.56 0.78 0.66 0.79 0.76 0.15 0.05 0 – 0.05 0.13 0.49 0.39 0.48 0.48 0.42 0.35
Nucleus 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0.05 0.09 0 0 0 0 0 0 0 0 0.05 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.05 0 0.06 0 0.19 0.11 0.08 0.09 0.09 0.13 0 0 0 – 0 0 0 0 0.05 0 0.07 0
Vac/Vac Membrane 0.11 0 0 0 0.11 0 0.13 0.08 0.05 0.12 0.06 0 0 0.08 0.06 – 0.07 0 0 0.06 0 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 3 0 1 0 0 0 1 0 0 0 0 4 0 4 – 0 1 7 10 1 8 13 10
Bud 2 3 0 1 2 11 2 8 13 13 19 18 2 1 3 – 1 1 2 0 1 3 4 10
Bud Neck 1 1 4 0 0 0 0 4 2 4 4 4 2 0 0 – 1 2 4 4 1 3 6 10
Bud Site 0 0 0 0 0 0 0 2 4 0 6 4 0 0 0 – 0 0 – – – – – –
Cell Periphery 1 1 0 2 1 0 5 0 2 3 3 1 2 2 6 – 6 7 0 4 1 1 1 4
Cytoplasm 162 177 84 105 132 69 94 110 66 73 49 36 157 288 321 – 170 148 65 102 48 54 107 133
Endoplasmic Reticulum 4 3 1 0 0 0 1 0 1 0 1 1 24 0 3 – 10 12 5 1 2 2 3 4
Endosome 8 1 1 12 48 5 46 44 15 44 9 31 9 43 37 – 7 10 36 17 5 13 20 19
Golgi 3 0 1 0 1 0 0 1 0 0 1 0 0 0 0 – 0 0 4 0 0 1 0 0
Mitochondria 59 35 3 25 26 170 144 222 290 239 258 264 35 19 6 – 10 25 154 110 70 112 152 126
Nucleus 0 0 0 0 1 11 11 16 11 3 8 7 0 0 1 – 3 2 0 0 0 0 1 1
Nuclear Periphery 0 0 0 0 0 0 2 0 0 2 0 0 0 0 0 – 0 0 2 0 0 2 1 1
Nucleolus 0 0 0 0 0 4 1 0 2 4 2 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 1 0 0 7 21 0 10 3 1 15 6 15 0 18 8 – 3 4 0 0 1 2 1 0
SpindlePole 4 1 5 5 14 9 65 43 29 32 30 44 4 2 7 – 2 7 11 6 6 10 24 14
Vac/Vac Membrane 26 6 2 6 26 8 47 33 20 43 18 13 6 29 22 – 13 4 8 16 2 9 9 20
Unique Cell Count 241 215 95 149 239 238 350 397 373 361 326 347 229 368 387 198 194 315 284 148 235 359 365
Labelled Cell Count 275 231 101 164 272 287 428 487 456 475 414 438 245 402 418 226 223 315 284 148 235 359 365


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 4.2 5.1 5.8 5.9 4.1 5.2 4.9 4.3 4.5 4.2 4.4 4.8 5.2 5.3 4.7 5.5 4.9 4.4 4.5 4.5
Std Deviation (1e-4) 0.6 0.7 1.6 7.6 2.1 1.8 2.2 1.8 1.5 1.5 1.5 1.2 0.7 1.5 1.3 0.8 1.3 1.0 1.3 1.4 1.2
Intensity Change (Log2) – – – 0.2 0.21 -0.31 0.04 -0.06 -0.25 -0.16 -0.26 -0.21 -0.08 0.02 0.05 -0.12 0.11 -0.05 -0.2 -0.19 -0.19


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.4426 0.6294 0.6379 0.1588 -0.0309 0.6507 1.5378 2.0265 1.6799 1.7938 1.8178 1.7667 3.9974 3.8454 3.8128 4.5722 4.1952 4.2062
Actin 0.0227 0.0118 0.0104 0.0023 0.006 0.0112 0.0385 0.0119 0.0144 0.0236 0.0133 0.0131 0.0482 0.041 0.0911 0.0405 0.0414 0.0403
Bud 0.0015 0.004 0.0041 0.0066 0.0011 0.0021 0.0015 0.0059 0.0048 0.0039 0.0054 0.0021 0.0021 0.008 0.0047 0.0049 0.0042 0.0017
Bud Neck 0.004 0.0021 0.0023 0.0104 0.0021 0.0056 0.0043 0.0017 0.0063 0.001 0.0019 0.0094 0.0122 0.0028 0.0023 0.0047 0.0017 0.0063
Bud Periphery 0.0026 0.0069 0.0115 0.0185 0.0031 0.0052 0.0024 0.0102 0.0098 0.0089 0.0119 0.0044 0.0033 0.0154 0.0096 0.0102 0.0126 0.0043
Bud Site 0.0058 0.0239 0.0188 0.0074 0.0037 0.0024 0.0057 0.0361 0.021 0.0027 0.0047 0.0031 0.0207 0.0563 0.0218 0.0057 0.0058 0.0018
Cell Periphery 0.0018 0.0008 0.0013 0.0014 0.0008 0.0009 0.0013 0.0028 0.0021 0.0007 0.0009 0.002 0.0027 0.0042 0.0063 0.0038 0.0057 0.0018
Cytoplasm 0.0343 0.0923 0.042 0.1144 0.0445 0.0771 0.0541 0.1325 0.0787 0.0737 0.0937 0.0861 0.0655 0.1689 0.1307 0.1128 0.0536 0.0988
Cytoplasmic Foci 0.0997 0.151 0.0998 0.0474 0.1207 0.1137 0.0975 0.1244 0.0956 0.0974 0.1234 0.1124 0.1007 0.1289 0.0974 0.1146 0.0653 0.0971
Eisosomes 0.0005 0.0003 0.0005 0.0004 0.0004 0.0004 0.001 0.0006 0.0009 0.0003 0.0002 0.0004 0.0008 0.0028 0.0041 0.0042 0.0019 0.0007
Endoplasmic Reticulum 0.0105 0.0041 0.0024 0.0061 0.0062 0.0055 0.0221 0.0071 0.0072 0.005 0.0052 0.0083 0.0106 0.0088 0.0066 0.0017 0.0067 0.0072
Endosome 0.2408 0.2228 0.124 0.1321 0.1979 0.171 0.2559 0.1842 0.2216 0.1922 0.2149 0.1986 0.1988 0.1748 0.1091 0.1485 0.2099 0.1405
Golgi 0.0726 0.0951 0.0509 0.047 0.0676 0.0711 0.0873 0.0869 0.0861 0.0926 0.0754 0.0945 0.1145 0.088 0.08 0.0709 0.0937 0.0971
Lipid Particles 0.0326 0.0195 0.0115 0.0028 0.0627 0.0187 0.0424 0.013 0.014 0.0092 0.006 0.0111 0.0516 0.0086 0.0059 0.032 0.021 0.0094
Mitochondria 0.3427 0.2631 0.5144 0.4227 0.3311 0.4134 0.2849 0.2683 0.3539 0.4328 0.341 0.3708 0.2187 0.1808 0.2938 0.2696 0.3903 0.3262
None 0.0586 0.0419 0.0489 0.1289 0.0379 0.0513 0.0319 0.0488 0.0251 0.0096 0.0273 0.0235 0.0379 0.0673 0.0953 0.0919 0.0104 0.1169
Nuclear Periphery 0.013 0.0022 0.0016 0.0019 0.0061 0.0033 0.0099 0.0043 0.002 0.0015 0.0233 0.0019 0.0252 0.0012 0.0017 0.0003 0.0018 0.0008
Nucleolus 0.0018 0.001 0.0012 0.0023 0.003 0.0008 0.0012 0.0007 0.0008 0.0004 0.0006 0.0006 0.0012 0.0011 0.0007 0.0005 0.0007 0.0003
Nucleus 0.0038 0.0018 0.0019 0.006 0.0051 0.002 0.0024 0.0025 0.0017 0.0011 0.0024 0.0016 0.006 0.0016 0.0013 0.0008 0.0015 0.0009
Peroxisomes 0.0264 0.0332 0.0348 0.0071 0.0713 0.0276 0.0258 0.0307 0.0229 0.0239 0.0324 0.0385 0.0513 0.0177 0.0237 0.0645 0.0337 0.036
Punctate Nuclear 0.0044 0.0012 0.0016 0.0013 0.0117 0.0018 0.0048 0.0009 0.0013 0.0012 0.0012 0.0015 0.0012 0.0007 0.0008 0.0006 0.0008 0.0006
Vacuole 0.0129 0.015 0.011 0.0245 0.011 0.0094 0.0173 0.0196 0.0229 0.0109 0.0092 0.0095 0.0179 0.016 0.0095 0.0144 0.0253 0.0073
Vacuole Periphery 0.0073 0.0061 0.005 0.0085 0.0061 0.0054 0.0078 0.0066 0.0069 0.0072 0.0057 0.0068 0.0087 0.0051 0.0037 0.0032 0.012 0.004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 2.0433 7.3065 12.1989 5.5991 6.6232 10.2111 6.0024 4.9861 5.2054 4.8885
Translational Efficiency 4.2043 0.9015 0.6618 1.7412 0.997 1.1017 1.5709 1.9339 1.7521 1.4306

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
748 620 1286 98 1576 2300 1282 138 2324 2920 2568 236

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 608.13 730.16 855.85 938.67 654.12 786.59 880.83 972.11 639.32 774.61 868.32 958.22
Standard Deviation 71.94 101.52 96.81 103.25 73.83 97.73 102.73 107.38 76.32 101.21 100.59 106.96
Intensity Change Log 2 — 0.263833 0.492978 0.626238 — 0.266057 0.429308 0.571564 — 0.264986 0.460334 0.598164

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000356 0.002596 0.002922 0.002823 0.000246 0.000492 0.005108 0.022389 0.000282 0.000939 0.004013 0.014264
Bud Neck 0.050064 0.057203 0.027511 0.009665 0.027713 0.065682 0.026498 0.014386 0.034907 0.063882 0.027005 0.012425
Bud Site 0.011360 0.061001 0.044386 0.032667 0.007604 0.052444 0.086875 0.113496* 0.008813 0.054261 0.065597 0.079932
Cell Periphery 0.000233 0.000330 0.000187 0.000235 0.000217 0.000214 0.000183 0.000374 0.000222 0.000239 0.000185 0.000316
Cytoplasm 0.257588* 0.171017* 0.284906* 0.182504* 0.337782* 0.148001* 0.280401* 0.208887* 0.311971* 0.152888* 0.282657* 0.197931*
Cytoplasmic Foci 0.383100* 0.296680* 0.152041 0.204417* 0.344478* 0.215609* 0.202960* 0.188581* 0.356909* 0.232823* 0.177461 0.195157*
Eisosomes 0.000332 0.000227 0.000095 0.000215 0.000322 0.000139 0.000107 0.000246 0.000325 0.000158 0.000101 0.000233
Endoplasmic Reticulum 0.002218 0.005183 0.007597 0.003898 0.001757 0.001053 0.004368 0.006112 0.001906 0.001930 0.005985 0.005193
Endosome 0.037984 0.081418 0.033381 0.061625 0.024817 0.075398 0.042403 0.026356 0.029055 0.076677 0.037885 0.041002
Golgi 0.012799 0.039128 0.013086 0.004598 0.012376 0.040328 0.030715 0.022455 0.012512 0.040073 0.021887 0.015040
Lipid Particles 0.024438 0.012257 0.006615 0.010045 0.024236 0.007415 0.003572 0.006402 0.024301 0.008443 0.005096 0.007915
Mitochondria 0.054924 0.078593 0.028994 0.001025 0.037793 0.196791* 0.035442 0.009041 0.043307 0.171694* 0.032213 0.005712
Mitotic Spindle 0.000865 0.011020 0.062934 0.015471 0.000742 0.018588 0.036629 0.030179 0.000782 0.016981 0.049802 0.024071
None 0.004445 0.003337 0.001850 0.000532 0.001843 0.001912 0.000837 0.001310 0.002681 0.002214 0.001344 0.000987
Nuclear Periphery 0.000637 0.000341 0.002553 0.000761 0.000616 0.000376 0.000783 0.000562 0.000623 0.000369 0.001670 0.000645
Nuclear Periphery Foci 0.000279 0.000625 0.002236 0.004028 0.000252 0.000283 0.001955 0.003930 0.000261 0.000356 0.002096 0.003971
Nucleolus 0.003051 0.003236 0.003069 0.007554 0.001367 0.002139 0.001381 0.001317 0.001909 0.002372 0.002226 0.003907
Nucleus 0.072850 0.031686 0.167469* 0.062089 0.111498 0.032032 0.054314 0.078433 0.099059 0.031958 0.110980 0.071646
Peroxisomes 0.037731 0.061571 0.025800 0.030300 0.014166 0.025246 0.044003 0.094571 0.021751 0.032959 0.034887 0.067882
Vacuole 0.040512 0.071234 0.124998 0.363700* 0.046349 0.098741 0.133798 0.167140 0.044470 0.092900 0.129391 0.248762*
Vacuole Periphery 0.004232 0.011316 0.007370 0.001846 0.003824 0.017115 0.007670 0.003834 0.003955 0.015884 0.007520 0.003009

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.35 -9.22 -3.80 0.08 0.67 -4.35 -9.22 -4.02 -3.96 -2.94 -4.40 -12.60 -4.28 -4.02 -2.88
Bud Neck -1.18 5.55 10.49 9.85 8.39 -12.65 0.43 5.15 16.45 4.91 -10.56 3.80 10.86 20.44 8.74
Bud Site -10.29 -11.05 -2.16 4.40 2.36 -18.61 -19.40 -8.09 -3.87 -1.24 -21.09 -22.33 -7.94 -1.61 -0.63
Cell Periphery -2.30 2.49 0.09 1.98 -1.40 0.17 2.35 -2.55 -2.55 -3.52 -0.84 3.19 -2.19 -1.53 -3.71
Cytoplasm 6.77 -2.08 3.01 -0.57 4.09 24.61 5.25 5.69 -3.73 3.02 24.32 3.86 6.77 -3.53 4.90
Cytoplasmic Foci 7.03 24.61 11.28 6.82 -0.92 17.99 19.81 12.31 3.66 1.83 20.11 31.86 16.47 6.13 0.38
Eisosomes 3.94 10.55 3.91 0.51 -5.51 8.39 10.65 3.02 -3.47 -5.03 9.81 14.44 4.60 -3.54 -7.23
Endoplasmic Reticulum -1.36 -6.94 -2.58 0.21 3.93 2.45 -5.55 -4.96 -5.92 -1.88 -0.06 -10.18 -5.73 -5.01 0.91
Endosome -7.91 2.23 -1.33 3.95 -2.41 -19.37 -5.85 0.58 13.97 4.46 -19.90 -3.08 -1.14 10.80 0.43
Golgi -7.50 0.84 5.86 10.33 5.14 -14.08 -6.56 -0.60 5.20 2.45 -16.05 -4.88 0.87 9.60 3.13
Lipid Particles 4.89 9.05 5.34 0.80 -1.90 11.40 15.24 9.84 0.84 -2.12 12.71 17.00 10.73 0.44 -2.57
Mitochondria -2.92 5.48 11.34 12.12 11.15 -26.88* 1.48 7.40 30.49* 5.97 -25.38* 4.80 12.77 34.37* 9.46
Mitotic Spindle -5.16 -14.21 -2.88 -0.68 7.29 -11.01 -11.51 -3.91 -1.53 0.74 -12.12 -18.17 -4.72 -1.36 4.47
None 0.66 3.15 4.62 1.65 5.39 -0.10 5.50 1.43 1.24 -0.73 0.94 4.47 4.51 2.41 1.42
Nuclear Periphery 2.09 -8.96 -2.11 -4.42 7.79 2.34 -1.78 -0.53 -2.03 0.90 3.06 -8.58 -1.55 -3.98 6.57
Nuclear Periphery Foci -2.86 -7.46 -5.74 -5.27 -3.11 -0.54 -3.24 -3.93 -3.90 -2.10 -1.79 -6.26 -6.16 -6.02 -3.29
Nucleolus -0.08 0.30 -0.46 -0.44 -0.52 -2.65 -0.38 -0.00 1.12 0.15 -1.19 -0.84 -0.55 -0.32 -0.40
Nucleus 7.85 -13.71 0.20 -3.64 8.97 19.06 9.47 0.62 -4.90 -2.60 20.21 -4.71 1.00 -6.01 3.19
Peroxisomes -4.54 3.70 3.00 6.43 0.66 -7.94 -9.67 -5.48 -4.51 -3.12 -6.51 -5.63 -4.47 -2.92 -2.93
Vacuole -6.23 -19.10 -13.18 -11.98 -9.72 -14.16 -18.92 -9.14 -6.07 -3.07 -15.87 -26.59 -14.57 -11.67 -8.66
Vacuole Periphery -6.15 -2.70 5.23 9.09 6.64 -16.62 -4.83 0.96 10.50 3.29 -17.62 -5.33 2.53 14.73 5.57
Standard name
Human Ortholog
Description Mitochondrial protein of unknown function; similar to Ups1p and Ups2p which are involved in regulation of mitochondrial cardiolipin and phosphatidylethanolamine levels; null is viable but interacts synthetically with ups1 and ups2 mutations; UPS3 has a paralog, UPS2, that arose from the whole genome duplication
Localization
Cell Percentages mitochondrion (18%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Ups3

Ups3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ups3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available