Standard name | |
---|---|
Human Ortholog | |
Description | Vacuolar membrane protein; vacuole-specific Myo2p receptor and Myo2p-Vac17p-Vac8p transport complex subunit required for vacuolar inheritance; required with Atg13p for the vesicle closure step of the cytoplasm-to-vacuole (CVT) pathway, for homotypic vacuole-vacuole fusion and for nucleus-vacuole junction formation with Nvy1p; contains 11 armadillo (ARM) repeats; myristoylated, palmitoylated, and phosphorylated |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 7 | – | 3 | 6 | 3 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Bud | 0 | 0 | 2 | 0 | 1 | 1 | 1 | 4 | – | 1 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | – | 2 | 4 | 2 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 0 | 12 | 8 | 15 | 10 | 19 | 29 | – | 31 | 39 | 29 | 9 | 13 | 17 | 19 | 12 | 11 | 0 | 0 | 0 | 0 | 0 | 1 |
Endoplasmic Reticulum | 0 | 0 | 2 | 0 | 0 | 7 | 12 | 16 | – | 25 | 10 | 17 | 1 | 4 | 0 | 11 | 1 | 11 | 0 | 0 | 0 | 0 | 1 | 1 |
Endosome | 14 | 1 | 30 | 49 | 33 | 18 | 19 | 44 | – | 19 | 11 | 30 | 76 | 45 | 49 | 23 | 19 | 13 | 25 | 10 | 92 | 16 | 22 | 27 |
Golgi | 12 | 4 | 14 | 11 | 47 | 49 | 93 | 86 | – | 63 | 24 | 66 | 44 | 53 | 71 | 17 | 12 | 24 | 1 | 1 | 3 | 0 | 3 | 1 |
Mitochondria | 26 | 15 | 109 | 103 | 36 | 95 | 105 | 180 | – | 157 | 123 | 123 | 27 | 11 | 10 | 14 | 12 | 9 | 6 | 1 | 24 | 6 | 4 | 4 |
Nucleus | 9 | 4 | 1 | 1 | 3 | 8 | 4 | 4 | – | 2 | 4 | 1 | 9 | 20 | 23 | 14 | 11 | 2 | 3 | 4 | 1 | 9 | 10 | 16 |
Nuclear Periphery | 11 | 4 | 13 | 11 | 7 | 17 | 19 | 22 | – | 8 | 8 | 7 | 30 | 16 | 12 | 20 | 20 | 21 | 10 | 1 | 42 | 8 | 15 | 9 |
Nucleolus | 74 | 23 | 159 | 113 | 59 | 64 | 51 | 87 | – | 58 | 47 | 40 | 289 | 219 | 296 | 62 | 39 | 56 | 13 | 5 | 8 | 9 | 13 | 23 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 11 | 3 | 4 | 5 | 4 | 2 | 1 | 0 | 3 | 2 | 0 | 0 |
Vac/Vac Membrane | 179 | 71 | 218 | 128 | 189 | 227 | 244 | 240 | – | 196 | 171 | 243 | 581 | 707 | 868 | 208 | 107 | 153 | 199 | 90 | 201 | 206 | 285 | 294 |
Unique Cell Count | 244 | 90 | 365 | 275 | 272 | 364 | 428 | 558 | 419 | 350 | 435 | 830 | 876 | 1063 | 287 | 154 | 199 | 263 | 117 | 385 | 263 | 361 | 388 | |
Labelled Cell Count | 330 | 122 | 560 | 425 | 391 | 496 | 569 | 723 | 565 | 447 | 565 | 1078 | 1091 | 1352 | 394 | 237 | 303 | 263 | 117 | 385 | 263 | 361 | 388 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 21.9 | 24.6 | 22.3 | 20.2 | 20.7 | 18.1 | 17.6 | 17.3 | – | 16.4 | 16.0 | 17.5 | 21.4 | 24.0 | 25.9 | 14.9 | 15.2 | 21.7 | 24.2 | 26.4 | 27.8 |
Std Deviation (1e-4) | 5.5 | 7.2 | 6.0 | 5.2 | 5.7 | 5.0 | 4.6 | 4.6 | – | 3.9 | 3.8 | 4.3 | 6.0 | 6.4 | 6.4 | 5.3 | 6.7 | 8.2 | 7.1 | 8.4 | 8.6 |
Intensity Change (Log2) | – | – | – | -0.14 | -0.11 | -0.3 | -0.34 | -0.37 | – | -0.44 | -0.47 | -0.35 | -0.06 | 0.11 | 0.22 | -0.58 | -0.55 | -0.04 | 0.12 | 0.24 | 0.32 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.3 | 1.4 | -0.4 | 0.8 | 1.4 | – | 2.5 | 4.1 | 2.2 | -2.7 | -2.1 | -2.0 | 2.0 | 2.2 | 1.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 2.4 | 2.5 | – | 4.2 | 2.4 | 3.1 | 0 | 0 | 0 | 3.0 | 0 | 0 |
Endosome | 3.7 | 1.6 | -1.8 | -2.2 | -0.2 | – | -2.1 | -2.9 | -0.7 | 0.5 | -2.1 | -2.6 | -0.1 | 1.5 | -0.7 |
Golgi | 0.1 | 5.7 | 4.6 | 7.4 | 5.5 | – | 5.3 | 1.8 | 5.3 | 1.1 | 1.6 | 2.0 | 1.2 | 1.9 | 3.7 |
Mitochondria | 2.0 | -5.0 | -1.1 | -1.7 | 0.8 | – | 2.2 | 1.5 | -0.5 | -13.3 | -15.5 | -17.2 | -8.1 | -5.4 | -7.1 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 2.5 | 2.4 | 3.9 | 0 | 0 |
Nuclear Periphery | 0.3 | -0.7 | 0.8 | 0.6 | 0.3 | – | -1.4 | -1.0 | -1.8 | 0 | -1.8 | -3.1 | 2.0 | 4.0 | 3.3 |
Nucleolus | -0.6 | -5.8 | -7.6 | -10.1 | -9.4 | – | -9.3 | -8.9 | -11.2 | -2.9 | -6.5 | -5.6 | -5.9 | -3.9 | -3.6 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -3.3 | 2.5 | 0.7 | -0.8 | -5.0 | – | -3.6 | -2.9 | -1.1 | 3.5 | 7.7 | 8.5 | 3.4 | 2.1 | 4.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 14.5519 | 20.4514 | 18.5575 | 16.7711 | 17.2692 | 18.5163 | 18.5766 | 21.0952 | 18.9966 | 20.2168 | 19.9982 | 19.4878 | 18.4812 | 23.1801 | 21.4737 | 19.7743 | 19.2333 | 21.0324 |
Actin | 0.0026 | 0 | 0.0165 | 0.0015 | 0.0212 | 0 | 0.0154 | 0 | 0.0044 | 0 | 0 | 0.0083 | 0.0068 | 0 | 0.0012 | 0.0001 | 0 | 0.0004 |
Bud | 0.0003 | 0 | 0.0002 | 0.0003 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0.0002 | 0 | 0.0009 | 0.0002 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0017 | 0 | 0 | 0.0003 | 0.0019 | 0 | 0.0001 | 0 | 0 | 0 |
Bud Periphery | 0.0005 | 0.0001 | 0.0003 | 0.0006 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 |
Bud Site | 0.0007 | 0 | 0.008 | 0.0015 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 | 0.0003 | 0.0021 | 0 | 0.0001 | 0 | 0 | 0 |
Cell Periphery | 0.0032 | 0.0031 | 0.0016 | 0.0013 | 0.0008 | 0.0013 | 0.0016 | 0.0025 | 0.0019 | 0.0007 | 0.0003 | 0.0012 | 0.0021 | 0.0011 | 0.0014 | 0.0023 | 0.001 | 0.0017 |
Cytoplasm | 0.0096 | 0.0002 | 0.0042 | 0.0005 | 0.0006 | 0.0001 | 0.0004 | 0.0001 | 0.0007 | 0 | 0 | 0.0002 | 0.0016 | 0 | 0.0004 | 0 | 0 | 0.0005 |
Cytoplasmic Foci | 0.0074 | 0.0006 | 0.0106 | 0.0018 | 0.0008 | 0.0137 | 0.0063 | 0.0017 | 0.0104 | 0.0008 | 0.0005 | 0.0065 | 0.0062 | 0.0009 | 0.0083 | 0.0002 | 0.0002 | 0.0017 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0028 | 0.0001 | 0.0006 | 0.0007 | 0.0023 | 0.0001 | 0.008 | 0.0001 | 0.0009 | 0 | 0.0001 | 0.0003 | 0.0022 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 |
Endosome | 0.0195 | 0.0042 | 0.0147 | 0.0063 | 0.0189 | 0.0035 | 0.0177 | 0.0066 | 0.0121 | 0.006 | 0.0059 | 0.0087 | 0.0188 | 0.0058 | 0.0077 | 0.0028 | 0.0057 | 0.0054 |
Golgi | 0.003 | 0.0003 | 0.0057 | 0.001 | 0.0028 | 0.0005 | 0.0128 | 0.0014 | 0.0094 | 0.001 | 0.0013 | 0.0064 | 0.0041 | 0.0012 | 0.0025 | 0.0004 | 0.0006 | 0.0015 |
Lipid Particles | 0.0429 | 0.018 | 0.0283 | 0.0144 | 0.006 | 0.0288 | 0.23 | 0.0819 | 0.0774 | 0.0704 | 0.0561 | 0.1554 | 0.0923 | 0.0563 | 0.0679 | 0.0337 | 0.0253 | 0.1094 |
Mitochondria | 0.0151 | 0.0014 | 0.009 | 0.0071 | 0.0015 | 0.0034 | 0.0241 | 0.0089 | 0.0467 | 0.0016 | 0.0023 | 0.0157 | 0.0314 | 0.0022 | 0.031 | 0.0616 | 0.0014 | 0.0039 |
None | 0.0025 | 0.0001 | 0.0009 | 0.0006 | 0.0008 | 0 | 0.0003 | 0 | 0.0041 | 0 | 0 | 0.0002 | 0.0049 | 0 | 0.003 | 0 | 0 | 0.0006 |
Nuclear Periphery | 0.0409 | 0.0108 | 0.0131 | 0.0063 | 0.0209 | 0.0067 | 0.0083 | 0.0219 | 0.0047 | 0.0014 | 0.004 | 0.0137 | 0.0134 | 0.0333 | 0.0102 | 0.0006 | 0.0139 | 0.0149 |
Nucleolus | 0.0221 | 0.0261 | 0.0224 | 0.0109 | 0.0021 | 0.0256 | 0.019 | 0.0248 | 0.0155 | 0.0033 | 0.0013 | 0.0088 | 0.0435 | 0.0072 | 0.0147 | 0.0081 | 0.0021 | 0.0309 |
Nucleus | 0.0323 | 0.0059 | 0.0093 | 0.0112 | 0.0018 | 0.0112 | 0.0028 | 0.0024 | 0.0039 | 0.0005 | 0.0004 | 0.0016 | 0.0064 | 0.0015 | 0.0036 | 0.001 | 0.001 | 0.0042 |
Peroxisomes | 0.0018 | 0.0001 | 0.0051 | 0.0005 | 0.0003 | 0.0001 | 0.0014 | 0.0003 | 0.0024 | 0.0001 | 0.0001 | 0.0036 | 0.0014 | 0.0001 | 0.0012 | 0.0001 | 0.0001 | 0.0003 |
Punctate Nuclear | 0.0031 | 0 | 0.0031 | 0.0047 | 0.0021 | 0 | 0.0001 | 0.0001 | 0.0055 | 0 | 0 | 0.0006 | 0.0033 | 0.0001 | 0.0048 | 0 | 0 | 0.0034 |
Vacuole | 0.2064 | 0.2596 | 0.2076 | 0.2576 | 0.1854 | 0.1272 | 0.0858 | 0.1508 | 0.1405 | 0.1564 | 0.0789 | 0.0939 | 0.1316 | 0.1296 | 0.1194 | 0.1708 | 0.1565 | 0.0852 |
Vacuole Periphery | 0.583 | 0.6694 | 0.6376 | 0.671 | 0.731 | 0.7775 | 0.5653 | 0.6963 | 0.6572 | 0.7576 | 0.8488 | 0.6741 | 0.6255 | 0.7606 | 0.7222 | 0.7184 | 0.7921 | 0.7355 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 62.5707 | 48.2683 | 66.1564 | 83.18 | 68.3597 | 60.314 | 71.34 | 75.9544 | 82.5317 | 79.8039 |
Translational Efficiency | 1.0308 | 1.3152 | 1.0247 | 1.0182 | 0.9361 | 1.0597 | 1.0921 | 0.9308 | 0.9892 | 0.8809 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
87 | 164 | 303 | 1588 | 164 | 1371 | 2745 | 1573 | 251 | 1535 | 3048 | 3161 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 2359.83 | 1846.04 | 2914.74 | 2299.48 | 1626.86 | 1689.78 | 2369.66 | 2198.01 | 1880.92 | 1706.47 | 2423.85 | 2248.99 |
Standard Deviation | 632.80 | 453.51 | 493.48 | 584.86 | 339.45 | 345.39 | 495.20 | 619.77 | 579.44 | 361.73 | 521.21 | 604.62 |
Intensity Change Log 2 | — | -0.354249 | 0.304684 | -0.037375 | — | 0.054745 | 0.542590 | 0.434108 | — | -0.173147 | 0.406548 | 0.173929 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000367 | 0.000201 | 0.000472 | 0.000165 | 0.000105 | 0.000490 | 0.000088 | 0.000261 | 0.000196 | 0.000459 | 0.000126 | 0.000213 |
Bud Neck | 0.013849 | 0.003876 | 0.006676 | 0.001138 | 0.001869 | 0.004984 | 0.000655 | 0.000679 | 0.006021 | 0.004866 | 0.001254 | 0.000909 |
Bud Site | 0.006698 | 0.001533 | 0.001133 | 0.001586 | 0.001048 | 0.001702 | 0.000490 | 0.000904 | 0.003006 | 0.001684 | 0.000554 | 0.001246 |
Cell Periphery | 0.000409 | 0.000292 | 0.000182 | 0.000755 | 0.000191 | 0.000294 | 0.001238 | 0.001369 | 0.000266 | 0.000294 | 0.001133 | 0.001060 |
Cytoplasm | 0.001345 | 0.000590 | 0.000125 | 0.000123 | 0.000500 | 0.000509 | 0.000362 | 0.000057 | 0.000793 | 0.000518 | 0.000338 | 0.000090 |
Cytoplasmic Foci | 0.067188 | 0.026707 | 0.038520 | 0.001561 | 0.028756 | 0.043695 | 0.001461 | 0.001069 | 0.042077 | 0.041880 | 0.005145 | 0.001316 |
Eisosomes | 0.000030 | 0.000032 | 0.000224 | 0.000035 | 0.000018 | 0.000109 | 0.000226 | 0.000032 | 0.000022 | 0.000101 | 0.000226 | 0.000034 |
Endoplasmic Reticulum | 0.000910 | 0.000200 | 0.000065 | 0.000219 | 0.000252 | 0.000352 | 0.000235 | 0.000341 | 0.000480 | 0.000336 | 0.000218 | 0.000280 |
Endosome | 0.012453 | 0.007176 | 0.000566 | 0.002004 | 0.012274 | 0.007928 | 0.000461 | 0.001828 | 0.012336 | 0.007848 | 0.000472 | 0.001916 |
Golgi | 0.043803 | 0.017943 | 0.002847 | 0.010021 | 0.027308 | 0.013286 | 0.001400 | 0.019607 | 0.033026 | 0.013783 | 0.001544 | 0.014791 |
Lipid Particles | 0.002669 | 0.010084 | 0.004977 | 0.004940 | 0.009028 | 0.026087 | 0.009061 | 0.005904 | 0.006824 | 0.024377 | 0.008655 | 0.005420 |
Mitochondria | 0.007431 | 0.012177 | 0.031343 | 0.013996 | 0.002652 | 0.041299 | 0.011079 | 0.011371 | 0.004309 | 0.038187 | 0.013094 | 0.012690 |
Mitotic Spindle | 0.014316 | 0.005947 | 0.011730 | 0.001080 | 0.002404 | 0.003658 | 0.000059 | 0.000205 | 0.006533 | 0.003903 | 0.001219 | 0.000644 |
None | 0.000493 | 0.001024 | 0.001968 | 0.000573 | 0.000213 | 0.001730 | 0.001045 | 0.000465 | 0.000310 | 0.001654 | 0.001137 | 0.000519 |
Nuclear Periphery | 0.031877 | 0.010915 | 0.020671 | 0.006114 | 0.003270 | 0.012285 | 0.003746 | 0.003652 | 0.013186 | 0.012138 | 0.005429 | 0.004889 |
Nuclear Periphery Foci | 0.037072 | 0.019929 | 0.012885 | 0.004535 | 0.019901 | 0.028421 | 0.002779 | 0.002912 | 0.025852 | 0.027514 | 0.003784 | 0.003728 |
Nucleolus | 0.035025 | 0.015483 | 0.138566 | 0.005003 | 0.003435 | 0.036127 | 0.009518 | 0.002119 | 0.014385 | 0.033922 | 0.022346 | 0.003568 |
Nucleus | 0.017102 | 0.001706 | 0.006133 | 0.000550 | 0.000532 | 0.004208 | 0.001400 | 0.000314 | 0.006276 | 0.003941 | 0.001870 | 0.000433 |
Peroxisomes | 0.001655 | 0.001176 | 0.004474 | 0.000118 | 0.001496 | 0.003406 | 0.000289 | 0.000054 | 0.001551 | 0.003168 | 0.000705 | 0.000086 |
Vacuole | 0.242195* | 0.200552* | 0.160583 | 0.295209* | 0.253603* | 0.210255* | 0.342640* | 0.299590* | 0.249649* | 0.209218* | 0.324542* | 0.297389* |
Vacuole Periphery | 0.463114* | 0.662458* | 0.555861* | 0.650275* | 0.631144* | 0.559175* | 0.611767* | 0.647270* | 0.572903* | 0.570210* | 0.606209* | 0.648780* |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.70 | -0.86 | 2.40 | 0.63 | 5.02 | -2.94 | 0.55 | -1.97 | 1.52 | -2.35 | -2.16 | 2.02 | -0.26 | 2.02 | -2.19 |
Bud Neck | 3.61 | 2.83 | 4.75 | 2.28 | 5.43 | -4.94 | 2.26 | 2.18 | 12.02 | -0.28 | 1.55 | 4.52 | 4.81 | 11.42 | 2.37 |
Bud Site | 2.26 | 2.44 | 2.25 | -0.03 | -0.74 | -1.28 | 1.04 | 0.29 | 2.99 | -1.36 | 1.66 | 2.75 | 2.05 | 1.36 | -2.01 |
Cell Periphery | 0.81 | 1.58 | -1.78 | -3.83 | -5.17 | -3.68 | -8.45 | -7.25 | -6.66 | -0.66 | -0.41 | -7.03 | -7.07 | -7.79 | 0.48 |
Cytoplasm | 1.92 | 3.38 | 3.38 | 1.68 | 0.00 | 0.01 | 0.28 | 1.75 | 7.15 | 1.05 | 1.56 | 1.41 | 3.48 | 6.57 | 0.96 |
Cytoplasmic Foci | 2.63 | 2.03 | 4.54 | 3.54 | 7.62 | -1.95 | 3.84 | 3.90 | 15.28 | 2.19 | 0.23 | 5.34 | 5.89 | 15.54 | 6.95 |
Eisosomes | -0.01 | -7.51 | -0.39 | -0.43 | 7.63 | -8.11 | -9.85 | -2.27 | 7.63 | 9.45 | -7.97 | -10.68 | -2.24 | 7.52 | 10.34 |
Endoplasmic Reticulum | 1.16 | 1.40 | 1.13 | -0.45 | -6.05 | -0.96 | 0.19 | -0.83 | 0.30 | -2.51 | 0.63 | 1.19 | 0.90 | 1.77 | -1.97 |
Endosome | 1.20 | 3.26 | 2.85 | 2.07 | -3.83 | 1.44 | 3.97 | 3.50 | 9.45 | -5.91 | 1.89 | 5.13 | 4.49 | 9.26 | -6.45 |
Golgi | 2.20 | 3.74 | 3.04 | 1.99 | -5.23 | 2.77 | 5.19 | 1.43 | -2.94 | -9.11 | 3.77 | 6.30 | 3.52 | -0.69 | -10.91 |
Lipid Particles | -2.77 | -1.97 | -2.13 | 1.96 | -0.00 | -5.22 | 0.01 | 1.14 | 10.89 | 4.90 | -7.20 | -0.94 | 0.79 | 11.29 | 5.66 |
Mitochondria | -0.80 | -4.93 | -1.87 | -0.35 | 4.20 | -13.57 | -7.47 | -5.17 | 9.47 | -0.17 | -12.15 | -6.00 | -5.11 | 9.15 | 0.26 |
Mitotic Spindle | 1.15 | 0.47 | 2.13 | 1.35 | 2.19 | -0.51 | 1.09 | 1.02 | 6.46 | -1.43 | 1.07 | 2.07 | 2.27 | 4.77 | 0.90 |
None | -1.93 | -8.79 | -0.46 | 1.89 | 11.37 | -19.69* | -20.59 | -8.10 | 16.31 | 14.02 | -16.06 | -14.92 | -4.15 | 15.28 | 15.46 |
Nuclear Periphery | 2.55 | 1.29 | 3.26 | 2.24 | 5.28 | -8.79 | -0.63 | -0.46 | 10.16 | 0.18 | 0.27 | 2.65 | 2.83 | 9.24 | 1.03 |
Nuclear Periphery Foci | 1.43 | 2.22 | 3.03 | 3.45 | 5.63 | -1.41 | 3.07 | 3.04 | 12.68 | -0.39 | -0.34 | 4.23 | 4.23 | 12.75 | 0.08 |
Nucleolus | 2.36 | -7.07 | 4.17 | 1.94 | 11.23 | -11.42 | -5.19 | 1.31 | 12.50 | 9.91 | -4.82 | -2.08 | 3.95 | 12.08 | 12.15 |
Nucleus | 1.54 | 1.14 | 1.65 | 1.93 | 5.38 | -7.37 | -3.84 | 1.27 | 8.19 | 6.51 | 0.74 | 1.29 | 1.67 | 8.15 | 7.78 |
Peroxisomes | 1.25 | -3.25 | 3.53 | 3.28 | 7.62 | -2.72 | 1.95 | 2.33 | 9.50 | 7.18 | -2.83 | 2.13 | 3.55 | 9.69 | 8.95 |
Vacuole | 2.11 | 4.46 | -2.80 | -6.90 | -13.73 | 2.97 | -6.08 | -3.09 | -13.87 | 6.63 | 3.60 | -6.36 | -4.08 | -16.78 | 5.11 |
Vacuole Periphery | -5.46 | -2.90 | -6.11 | 0.50 | -5.15 | 3.09 | 0.83 | -0.74 | -9.14 | -4.96 | -0.12 | -2.01 | -4.19 | -9.32 | -7.18 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|