Standard name
Human Ortholog
Description Endosomal SNARE related to mammalian syntaxin 8
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.94 0.93 1.0 0.98 0.97 0.88 0.83 – 0.7 0.73 0.65 0.66 1.0 0.99 0.99 0.94 0.97 0.96 0.92 0.89 0.93 0.92 0.92 0.95
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0 0 0 0.13 0.08 – 0.31 0.25 0.34 0.35 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.18 – 0.14 0.1 0.07 0.07 0 0 0 0.05 0 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 1 1 0 0 0 0 0 0 0 0 0 0 0 1 1 0
Bud 0 0 0 0 0 3 5 – 9 9 14 6 0 1 0 0 0 1 0 0 1 2 4 2
Bud Neck 0 0 0 0 0 0 0 – 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 1 2 1 8 – 5 7 6 11 1 0 5 6 6 9 0 0 0 0 0 0
Cytoplasm 76 69 48 242 361 255 505 – 311 354 272 321 211 561 601 224 195 243 80 81 78 269 396 437
Endoplasmic Reticulum 0 1 0 0 0 1 0 – 1 0 0 0 1 1 2 7 9 6 0 0 0 2 2 2
Endosome 0 0 0 0 0 0 2 – 0 0 0 0 0 1 0 0 0 0 1 1 0 1 2 2
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 1 1 1 0 0 0 1 0 1 3 2
Mitochondria 3 4 0 2 4 39 46 – 140 121 145 172 0 0 1 5 1 1 0 1 0 0 2 0
Nucleus 0 0 0 1 2 5 11 – 18 18 21 17 0 1 0 7 4 2 1 0 0 0 3 0
Nuclear Periphery 1 2 0 0 0 0 2 – 4 1 1 2 0 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 1 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 1
Vac/Vac Membrane 2 1 0 9 14 12 109 – 62 48 30 35 0 4 15 12 9 14 2 3 0 6 5 3
Unique Cell Count 81 74 48 248 373 289 611 445 488 421 485 211 565 610 239 202 254 88 91 84 292 431 459
Labelled Cell Count 83 77 48 255 383 316 688 552 561 491 565 213 570 626 262 224 276 88 91 84 292 431 459


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.7 5.6 6.4 6.2 7.0 5.1 6.1 – 5.3 5.4 5.2 5.4 7.0 7.2 7.4 8.0 8.4 8.5 6.1 6.4 6.5
Std Deviation (1e-4) 0.8 0.7 2.0 1.3 1.2 1.3 1.1 – 1.2 1.0 0.9 1.0 1.0 1.2 1.3 1.1 1.1 1.2 1.1 1.4 1.4
Intensity Change (Log2) – – – -0.04 0.12 -0.32 -0.06 – -0.26 -0.24 -0.3 -0.23 0.14 0.17 0.21 0.32 0.39 0.41 -0.06 -0.01 0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.1 -1.3 -2.5 -3.2 – -4.5 -4.2 -5.0 -4.8 0 -0.6 -0.8 -1.8 -1.3 -1.5
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 2.7 0 – 4.6 3.9 4.9 5.0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 3.2 – 2.8 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.4195 0.0726 0.1967 0.0093 -0.1692 0.507 0.9539 1.8383 1.3956 1.1043 1.1524 1.5681 2.5713 3.7381 2.9095 3.2805 2.8999 3.1873
Actin 0.0268 0.0026 0.0191 0.0105 0.0169 0.005 0.0369 0.0008 0.0051 0.085 0.0373 0.0058 0.0133 0.0001 0.0138 0.0034 0.0249 0.0069
Bud 0.001 0.0012 0.0025 0.0002 0.0003 0.0027 0.0005 0.0002 0.0003 0.0206 0.002 0.0001 0.0001 0 0.0003 0.0003 0.0001 0.0002
Bud Neck 0.0027 0.0005 0.0004 0.0444 0.0007 0.0014 0.0008 0.0002 0.0056 0.0015 0.0015 0.0006 0.0003 0.0001 0.0007 0.0007 0.0041 0.0024
Bud Periphery 0.0021 0.001 0.0032 0.0003 0.0003 0.0012 0.0007 0.0001 0.0003 0.0296 0.0034 0.0001 0.0001 0 0.0003 0.0002 0.0001 0.0002
Bud Site 0.003 0.0076 0.0053 0.001 0.001 0.002 0.0014 0.0023 0.0019 0.0617 0.0029 0.0001 0.0004 0.0003 0.0038 0.0004 0.0005 0.0002
Cell Periphery 0.0005 0.0002 0.0006 0.0002 0.0001 0.0006 0.0001 0.0001 0.0003 0.0007 0.0003 0.0001 0.0001 0.0001 0.0003 0 0.0002 0
Cytoplasm 0.4773 0.6836 0.6179 0.4959 0.6107 0.7015 0.6039 0.7754 0.7739 0.4622 0.6296 0.813 0.6106 0.7349 0.6334 0.702 0.6646 0.71
Cytoplasmic Foci 0.0341 0.0234 0.0195 0.0449 0.0483 0.0242 0.0141 0.0037 0.0071 0.0086 0.0256 0.0099 0.0076 0.0018 0.0086 0.0146 0.0125 0.0106
Eisosomes 0.0003 0.0001 0.0014 0.0002 0.0001 0 0.0003 0 0.0003 0.0022 0.001 0 0.0002 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0577 0.004 0.0291 0.0566 0.0385 0.0442 0.0033 0.003 0.0028 0.0029 0.0034 0.0051 0.0031 0.0013 0.0025 0.0023 0.0013 0.0016
Endosome 0.0314 0.0137 0.0086 0.0144 0.0207 0.0087 0.0099 0.0013 0.0047 0.0103 0.0331 0.0053 0.0052 0.0005 0.0042 0.0418 0.0065 0.0025
Golgi 0.0106 0.0007 0.0025 0.0012 0.0032 0.0027 0.0045 0.0001 0.0004 0.0065 0.0143 0.0014 0.0025 0 0.001 0.0071 0.0022 0.0016
Lipid Particles 0.0098 0.0012 0.0055 0.0018 0.0043 0.008 0.0067 0.0001 0.0002 0.0055 0.0355 0.0019 0.0055 0 0.0007 0.01 0.0296 0.0015
Mitochondria 0.0095 0.0005 0.0045 0.0007 0.0016 0.0017 0.003 0.0002 0.0005 0.0244 0.0305 0.0002 0.0017 0.0001 0.0006 0.0013 0.0009 0.003
None 0.2998 0.2425 0.2445 0.3123 0.2343 0.1661 0.3013 0.2058 0.1893 0.2486 0.109 0.138 0.3264 0.2572 0.3145 0.2037 0.2346 0.2469
Nuclear Periphery 0.0065 0.0028 0.0091 0.0056 0.003 0.0028 0.0021 0.0021 0.0012 0.0038 0.0091 0.0066 0.0047 0.0005 0.0011 0.0019 0.0012 0.0006
Nucleolus 0.0026 0.0003 0.0006 0.0002 0.0004 0.0007 0.0002 0.0001 0.0001 0.0011 0.0012 0.0001 0.0004 0 0.0004 0.0001 0.0019 0
Nucleus 0.0066 0.0072 0.0096 0.0029 0.0031 0.0099 0.0023 0.0028 0.0034 0.0072 0.0076 0.0071 0.0022 0.0023 0.004 0.0025 0.0022 0.0041
Peroxisomes 0.0066 0.0005 0.0084 0.0014 0.0017 0.0015 0.0045 0.0001 0.0003 0.0113 0.0197 0.0003 0.0095 0 0.0042 0.0044 0.0072 0.0047
Punctate Nuclear 0.0067 0.0034 0.0043 0.0029 0.0087 0.0127 0.0022 0.0007 0.0007 0.0018 0.0255 0.0035 0.0048 0.0002 0.0015 0.0022 0.0007 0.0026
Vacuole 0.0032 0.0026 0.0025 0.0016 0.0017 0.0019 0.0009 0.0007 0.0015 0.0031 0.0044 0.0006 0.0008 0.0004 0.0037 0.0007 0.003 0.0003
Vacuole Periphery 0.0012 0.0004 0.0007 0.0007 0.0004 0.0003 0.0003 0.0001 0.0003 0.0013 0.0031 0.0002 0.0003 0 0.0002 0.0005 0.0016 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 25.1427 12.8434 31.4503 34.1409 25.1475 27.2827 30.5171 33.3057 37.2869 30.6298
Translational Efficiency 0.7931 1.6176 0.6193 0.6021 0.6665 0.8408 0.6806 0.584 0.536 0.6566

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
261 707 1387 1468 1713 2199 1638 1547 1974 2906 3025 3015

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 694.24 843.97 1053.38 863.49 722.27 825.23 893.76 949.31 718.56 829.79 966.95 907.52
Standard Deviation 87.65 127.02 133.72 129.11 90.16 110.19 127.93 111.78 90.33 114.79 152.93 127.94
Intensity Change Log 2 — 0.281757 0.601520 0.314745 — 0.192258 0.307349 0.394341 — 0.236816 0.459016 0.355879

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000208 0.000571 0.000315 0.000344 0.000157 0.000177 0.000767 0.000451 0.000164 0.000273 0.000560 0.000399
Bud Neck 0.019449 0.007106 0.004160 0.005649 0.006871 0.014468 0.003315 0.005938 0.008534 0.012677 0.003702 0.005797
Bud Site 0.014384 0.020591 0.008964 0.025985 0.005310 0.012311 0.009950 0.040367 0.006509 0.014326 0.009497 0.033365
Cell Periphery 0.000350 0.000154 0.000113 0.000125 0.000101 0.000078 0.000102 0.000109 0.000134 0.000097 0.000107 0.000117
Cytoplasm 0.456174* 0.577581* 0.448204* 0.668949* 0.633401* 0.617729* 0.639050* 0.641029* 0.609968* 0.607961* 0.551545* 0.654624*
Cytoplasmic Foci 0.326699* 0.179235 0.005274 0.012990 0.144864 0.103908 0.012318 0.010262 0.168906 0.122234 0.009089 0.011590
Eisosomes 0.000199 0.000066 0.000017 0.000023 0.000059 0.000045 0.000023 0.000020 0.000078 0.000050 0.000020 0.000022
Endoplasmic Reticulum 0.002289 0.001920 0.002691 0.001426 0.000835 0.000472 0.001666 0.001407 0.001027 0.000824 0.002136 0.001416
Endosome 0.012750 0.013923 0.001434 0.002296 0.006722 0.009706 0.002717 0.002051 0.007519 0.010732 0.002129 0.002170
Golgi 0.007455 0.003115 0.000117 0.001787 0.001489 0.001952 0.001907 0.000388 0.002278 0.002235 0.001086 0.001070
Lipid Particles 0.006235 0.002917 0.000699 0.000632 0.002387 0.001767 0.000757 0.000596 0.002896 0.002047 0.000731 0.000614
Mitochondria 0.022612 0.001248 0.000798 0.000826 0.001435 0.002759 0.000665 0.001157 0.004235 0.002391 0.000726 0.000996
Mitotic Spindle 0.000731 0.003479 0.008362 0.017607 0.001272 0.005293 0.017931 0.023879 0.001201 0.004852 0.013543 0.020825
None 0.003921 0.002656 0.005669 0.006862 0.004245 0.005086 0.006960 0.006073 0.004202 0.004495 0.006368 0.006457
Nuclear Periphery 0.000258 0.000263 0.001546 0.000575 0.000272 0.000333 0.000859 0.000813 0.000270 0.000316 0.001174 0.000697
Nuclear Periphery Foci 0.000941 0.001686 0.002230 0.002103 0.000598 0.000396 0.001828 0.001606 0.000643 0.000710 0.002012 0.001848
Nucleolus 0.000994 0.000760 0.000208 0.000284 0.000531 0.001559 0.000209 0.000197 0.000592 0.001364 0.000208 0.000239
Nucleus 0.074595 0.094834 0.460192* 0.162510* 0.144650* 0.145148* 0.216459* 0.182891* 0.135387* 0.132907* 0.328214* 0.172968*
Peroxisomes 0.007025 0.006428 0.000299 0.000346 0.002703 0.003626 0.000308 0.000366 0.003275 0.004308 0.000304 0.000356
Vacuole 0.037524 0.079771 0.048351 0.087859 0.041356 0.072023 0.081855 0.079859 0.040849 0.073908 0.066493 0.083754
Vacuole Periphery 0.005207 0.001694 0.000356 0.000822 0.000742 0.001165 0.000355 0.000538 0.001332 0.001294 0.000355 0.000676

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.61 -6.96 -3.60 1.96 1.69 -0.46 -1.70 -4.16 -3.72 0.73 -2.28 -2.73 -4.90 -1.97 1.01
Bud Neck 4.48 5.56 5.58 3.35 0.44 -6.22 3.76 2.43 8.77 -1.64 -3.95 5.77 4.95 9.47 -0.81
Bud Site -1.82 3.01 -0.20 1.73 -3.54 -4.70 -1.54 -5.91 -2.80 -4.94 -5.77 -0.62 -6.52 -1.69 -6.06
Cell Periphery 4.35 4.98 5.53 4.16 3.07 4.08 1.25 2.42 -1.78 1.05 4.67 3.04 4.84 0.15 2.84
Cytoplasm -5.94 4.76 -4.41 2.87 -16.76 2.24 11.69 12.60 10.53 0.68 0.74 20.10 10.72 10.66 -10.44
Cytoplasmic Foci 9.49 23.13 22.62 23.63 -9.91 7.90 32.73 33.11 29.67 1.44 9.03 38.14 37.52 37.02 -4.69
Eisosomes 7.23 9.69 9.34 7.45 -3.67 4.92 11.07 12.35 8.41 1.47 7.45 14.54 14.06 10.50 -1.63
Endoplasmic Reticulum 0.45 -2.25 0.48 0.01 10.07 2.14 -6.78 -4.24 -11.80 3.82 1.05 -9.84 -4.02 -9.63 10.09
Endosome -0.53 6.86 6.97 9.86 1.02 -3.22 7.23 8.60 12.33 2.40 -3.80 9.45 10.30 15.45 2.51
Golgi 1.98 3.36 2.99 2.70 -1.11 -0.99 1.37 4.78 4.95 1.48 0.20 4.07 3.99 4.28 0.14
Lipid Particles 4.24 7.15 7.28 5.84 1.33 2.86 7.83 8.51 6.46 1.94 3.89 10.18 10.75 8.49 2.23
Mitochondria 3.80 3.88 3.92 2.02 1.71 -2.57 3.85 2.93 4.51 -1.95 2.23 4.56 4.57 4.96 0.13
Mitotic Spindle -2.03 -5.22 -6.55 -4.51 -2.19 -3.29 -6.01 -6.37 -4.29 -1.04 -3.61 -7.46 -8.74 -5.96 -2.18
None 1.02 -0.79 -0.97 -4.57 -0.57 -0.90 -1.83 -3.32 -2.24 -2.00 -0.29 -1.84 -2.91 -2.81 -1.85
Nuclear Periphery -0.18 -18.95 -9.46 -17.03 14.67 -1.46 -20.00 -18.21 -17.78 0.69 -1.34 -30.08 -21.74 -23.21 10.98
Nuclear Periphery Foci -1.30 -3.48 -2.24 -1.68 1.88 1.65 -8.39 -7.00 -7.25 2.59 -0.73 -11.78 -7.32 -6.71 2.82
Nucleolus 0.47 1.66 1.62 3.90 -0.34 -4.02 5.78 5.07 5.08 -0.58 -3.80 4.82 4.41 5.60 -0.61
Nucleus -2.67 -41.92 -16.95 -17.56 30.60 -0.27 -18.87 -16.09 -15.79 3.28 0.32 -39.49 -19.48 -20.60 22.86
Peroxisomes 0.49 6.22 6.22 6.92 -0.12 -1.40 5.52 5.60 8.14 0.66 -1.69 7.25 7.29 10.56 0.22
Vacuole -7.58 -9.42 -19.74 -13.45 -12.22 -10.16 -20.14 -22.29 -15.81 -2.26 -12.63 -22.08 -31.11 -21.75 -9.56
Vacuole Periphery 2.75 4.05 3.96 2.50 -0.54 -2.01 3.56 3.36 6.41 -0.57 0.27 5.10 4.68 5.55 -0.71
Standard name
Human Ortholog
Description Endosomal SNARE related to mammalian syntaxin 8
Localization
Cell Percentages cytoplasm (75%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Syn8

Syn8


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Syn8-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available