Standard name
Human Ortholog
Description PAS domain-containing serine/threonine protein kinase; coordinately regulates protein synthesis and carbohydrate metabolism and storage in response to a unknown metabolite that reflects nutritional status; PSK1 has a paralog, PSK2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0.06 0.05 0.07 0 0 0 0 0 0
Cytoplasm 0.98 0.97 0.96 0.96 0.99 0.95 0.83 0.88 0.84 0.76 0.75 0.74 1.0 1.0 0.99 0.93 0.92 0.96 0.95 0.91 0.78 0.92 0.93 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.05 0.15 0.08 0.2 0.4 0.27 0.49 0.44 0.51 0.52 0 0 0 0 0.05 0 0 0 0.1 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0.07 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 2 0 0 1 2
Bud 0 3 4 0 0 5 1 2 0 4 2 3 1 1 3 0 0 0 0 4 0 1 1 1
Bud Neck 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 1
Bud Site 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 – – – – – –
Cell Periphery 16 1 1 0 1 4 0 3 3 3 3 2 3 5 11 10 4 9 0 0 0 0 0 0
Cytoplasm 546 407 131 90 115 153 170 231 90 92 38 81 224 274 285 165 73 116 537 387 118 192 277 248
Endoplasmic Reticulum 6 2 1 1 0 0 0 2 0 0 0 0 0 0 0 4 6 5 0 1 2 1 4 1
Endosome 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 6 4 1 0 1 5
Golgi 2 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 2 1
Mitochondria 3 12 7 14 9 33 83 72 52 53 26 57 0 0 2 6 4 2 7 7 14 2 4 1
Nucleus 0 0 0 0 0 1 0 1 1 1 1 2 1 0 3 6 2 0 0 1 0 0 0 0
Nuclear Periphery 1 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 1 0
Nucleolus 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 0 0 0 1 0 0 1 0 0 0 0 0 1 0 0 1 0 0 0 0
Vac/Vac Membrane 1 1 0 0 0 0 1 0 0 1 2 1 0 0 0 10 2 5 0 4 10 8 1 3
Unique Cell Count 555 420 137 94 116 161 206 263 107 121 51 110 225 274 287 177 79 121 563 425 153 209 298 271
Labelled Cell Count 578 429 146 106 125 196 255 313 146 154 73 146 230 280 306 202 93 137 563 425 153 209 298 271


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.1 4.5 3.9 3.9 4.0 3.9 3.7 3.6 3.5 3.4 3.5 3.8 5.4 5.4 5.1 7.9 7.6 8.2 5.4 5.7 6.2
Std Deviation (1e-4) 0.6 0.7 0.8 1.1 0.6 1.1 1.3 1.0 1.7 1.3 0.9 1.7 1.3 0.9 1.0 2.1 2.0 2.4 1.1 1.4 1.8
Intensity Change (Log2) – – – 0.01 0.05 0.01 -0.08 -0.12 -0.16 -0.2 -0.17 -0.05 0.47 0.48 0.39 1.01 0.95 1.07 0.46 0.56 0.68


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.6531 2.6287 2.2198 1.4706 1.814 2.5969 0.2748 -0.1017 0.1307 -0.0524 -0.3934 0.0983 3.5364 4.8327 4.1509 3.808 4.1219 4.3109
Actin 0.018 0.0003 0.0003 0.0004 0.0014 0.0013 0.0348 0.0017 0.0196 0.0149 0.0067 0.007 0.0222 0.0013 0.0073 0.0035 0.0246 0.0034
Bud 0.0001 0.0001 0.0001 0.0001 0.0003 0.0001 0.0054 0.0108 0.0124 0.0143 0.0045 0.0027 0.0149 0.0138 0.0011 0.0229 0.0019 0.0078
Bud Neck 0.0005 0.0001 0.0002 0.0003 0.0005 0.0012 0.0114 0.0007 0.0069 0.0007 0.0041 0.0125 0.0004 0.0014 0.0005 0.0006 0.001 0.0004
Bud Periphery 0.0002 0 0 0.0001 0.0002 0.0002 0.0071 0.0081 0.0151 0.0201 0.0037 0.0032 0.0054 0.0098 0.0019 0.0287 0.0019 0.0045
Bud Site 0.0007 0.0008 0.0074 0.0001 0.0003 0.0001 0.0119 0.0283 0.0334 0.0072 0.0301 0.002 0.0142 0.092 0.0023 0.0092 0.002 0.0008
Cell Periphery 0.0007 0.0007 0.0005 0.0002 0.0002 0.0002 0.0009 0.0006 0.0006 0.0004 0.0008 0.0002 0.0008 0.0012 0.0004 0.0001 0.0001 0.0002
Cytoplasm 0.7323 0.8416 0.8233 0.975 0.8576 0.889 0.5348 0.703 0.6525 0.676 0.6389 0.7244 0.4441 0.5864 0.5354 0.8198 0.6741 0.5625
Cytoplasmic Foci 0.0047 0.0035 0.0035 0.0025 0.015 0.0049 0.0415 0.026 0.0297 0.0288 0.0397 0.0368 0.0181 0.0063 0.0096 0.0288 0.0241 0.0095
Eisosomes 0.0004 0.0001 0.0001 0 0 0.0001 0.0002 0 0.0001 0.0001 0.0001 0 0.0005 0.0001 0.0002 0 0.0001 0.0001
Endoplasmic Reticulum 0.0081 0.003 0.0046 0.0032 0.0059 0.0026 0.0102 0.0023 0.0054 0.0039 0.0034 0.0042 0.0028 0.0058 0.0023 0.0018 0.0034 0.0013
Endosome 0.006 0.0018 0.0023 0.0016 0.0309 0.0063 0.0528 0.0226 0.0297 0.0232 0.0438 0.0233 0.0046 0.0033 0.0045 0.0059 0.017 0.0023
Golgi 0.0018 0.0002 0.0003 0.0002 0.0011 0.0021 0.0088 0.0019 0.0027 0.0029 0.0017 0.0034 0.0023 0.0004 0.0017 0.0009 0.0022 0.0006
Lipid Particles 0.0007 0.0005 0.0003 0 0.0002 0.0001 0.0132 0.0005 0.0012 0.0009 0.0076 0.0026 0.0035 0.0002 0.0021 0.0001 0.0011 0.0003
Mitochondria 0.0008 0.0001 0.0001 0.0001 0.0003 0.0182 0.0095 0.0033 0.0031 0.005 0.0011 0.0019 0.0026 0.0004 0.0102 0.0012 0.0034 0.0007
None 0.2206 0.1447 0.1542 0.0133 0.0781 0.0714 0.1722 0.1742 0.166 0.1654 0.1779 0.1482 0.4422 0.2723 0.4046 0.0568 0.2227 0.3991
Nuclear Periphery 0.0016 0.0003 0.0004 0.0004 0.0008 0.0002 0.0203 0.0008 0.003 0.0089 0.0067 0.01 0.0005 0.001 0.0004 0.0006 0.0016 0.0008
Nucleolus 0.0001 0 0.0001 0 0 0 0.002 0.0005 0.0006 0.001 0.0014 0.0005 0.0005 0.0002 0.0001 0.0002 0.0003 0.0003
Nucleus 0.0007 0.0005 0.0007 0.0006 0.0007 0.0006 0.0334 0.0028 0.0041 0.008 0.0042 0.0046 0.0011 0.0015 0.0006 0.0013 0.0018 0.0018
Peroxisomes 0.0002 0 0 0 0.0001 0.0001 0.0065 0.0015 0.0036 0.0026 0.0015 0.004 0.0164 0.0001 0.0126 0.0128 0.0097 0.0009
Punctate Nuclear 0.0005 0.0001 0.0002 0 0.0002 0.0002 0.0056 0.0016 0.0018 0.0085 0.011 0.0028 0.001 0.0008 0.0003 0.0026 0.0037 0.0013
Vacuole 0.0013 0.0012 0.0012 0.0017 0.0054 0.0007 0.0136 0.0076 0.0069 0.0059 0.0094 0.0045 0.0017 0.0015 0.0016 0.0017 0.0027 0.001
Vacuole Periphery 0.0002 0.0001 0.0001 0.0001 0.0008 0.0003 0.004 0.001 0.0015 0.0013 0.0015 0.0011 0.0002 0.0002 0.0003 0.0003 0.0006 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.0427 19.6258 14.3834 21.7085 17.0436 19.5041 28.7854 30.7545 27.4033 22.4938
Translational Efficiency 0.5507 0.6191 0.5592 0.4941 0.441 0.5394 0.4974 0.4096 0.5212 0.4987

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
67 1057 223 1501 1974 1974 801 772 2041 3031 1024 2273

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 651.96 760.51 1042.80 911.09 717.65 764.39 883.85 910.07 715.49 763.04 918.47 910.74
Standard Deviation 83.35 104.57 122.37 116.83 81.52 95.52 102.79 116.26 82.42 98.79 125.82 116.63
Intensity Change Log 2 — 0.222184 0.677607 0.482810 — 0.091028 0.300521 0.342697 — 0.154949 0.492329 0.411093

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000133 0.000414 0.000472 0.000840 0.000119 0.000396 0.000068 0.001014 0.000119 0.000402 0.000156 0.000899
Bud Neck 0.015364 0.019674 0.001297 0.006878 0.011812 0.026050 0.001482 0.002906 0.011928 0.023826 0.001441 0.005529
Bud Site 0.008146 0.025932 0.009263 0.051602 0.008303 0.028293 0.005806 0.039517 0.008298 0.027470 0.006559 0.047497
Cell Periphery 0.000247 0.000094 0.000224 0.000093 0.000091 0.000096 0.000122 0.000087 0.000096 0.000095 0.000144 0.000091
Cytoplasm 0.504058* 0.472772* 0.616892* 0.620645* 0.596839* 0.531954* 0.621732* 0.588634* 0.593794* 0.511316* 0.620678* 0.609773*
Cytoplasmic Foci 0.268690* 0.272366* 0.051884 0.031520 0.206047* 0.213731* 0.048999 0.054268 0.208104* 0.234179* 0.049627 0.039246
Eisosomes 0.000273 0.000106 0.000058 0.000022 0.000087 0.000095 0.000057 0.000030 0.000093 0.000098 0.000057 0.000025
Endoplasmic Reticulum 0.001345 0.000582 0.002683 0.000806 0.001012 0.000339 0.000785 0.000565 0.001023 0.000424 0.001198 0.000724
Endosome 0.038317 0.050870 0.010819 0.015277 0.017082 0.036118 0.006206 0.006730 0.017779 0.041262 0.007211 0.012374
Golgi 0.003715 0.010089 0.000260 0.009284 0.004062 0.009772 0.000104 0.003252 0.004051 0.009883 0.000138 0.007235
Lipid Particles 0.009176 0.003196 0.002161 0.000971 0.003990 0.002226 0.001526 0.000664 0.004160 0.002564 0.001665 0.000867
Mitochondria 0.003893 0.004638 0.000099 0.007625 0.003817 0.005604 0.000231 0.004014 0.003819 0.005267 0.000202 0.006399
Mitotic Spindle 0.000177 0.005732 0.003023 0.071848 0.002350 0.011250 0.002626 0.041547 0.002278 0.009325 0.002712 0.061556
None 0.001389 0.000700 0.001339 0.003353 0.002076 0.001372 0.004511 0.000780 0.002053 0.001138 0.003820 0.002479
Nuclear Periphery 0.000217 0.000112 0.000661 0.000544 0.000165 0.000097 0.000595 0.000289 0.000167 0.000102 0.000610 0.000458
Nuclear Periphery Foci 0.000254 0.000469 0.008125 0.001969 0.000220 0.000160 0.001626 0.001254 0.000221 0.000268 0.003041 0.001726
Nucleolus 0.000647 0.000566 0.000157 0.000301 0.000490 0.000438 0.000354 0.000098 0.000495 0.000483 0.000311 0.000232
Nucleus 0.043117 0.016489 0.071411 0.038087 0.052881 0.018540 0.095447 0.025077 0.052561 0.017825 0.090212 0.033669
Peroxisomes 0.003330 0.009454 0.000330 0.001564 0.002444 0.007774 0.000232 0.003089 0.002473 0.008360 0.000253 0.002082
Vacuole 0.094808 0.102516 0.218525* 0.131308 0.084026 0.102655 0.207049* 0.222112* 0.084380 0.102606 0.209548* 0.162148
Vacuole Periphery 0.002704 0.003229 0.000316 0.005464 0.002087 0.003043 0.000443 0.004074 0.002107 0.003107 0.000415 0.004992

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.48 -4.16 -2.33 -0.29 0.41 -2.40 2.30 -2.53 -0.21 -2.88 -3.68 -2.47 -3.63 -0.37 -3.05
Bud Neck -1.23 4.72 4.08 11.03 -3.44 -9.16 13.77 12.73 17.54 -1.13 -9.46 14.31 11.54 19.75 -3.61
Bud Site -7.85 -0.54 -3.85 2.93 -4.11 -12.20 2.79 -3.18 4.12 -4.34 -14.80 2.34 -5.43 5.04 -6.64
Cell Periphery 4.76 0.99 5.99 6.65 9.84 -0.65 -2.09 7.28 7.15 8.12 0.30 -4.57 8.97 9.46 12.16
Cytoplasm 0.94 -0.78 -1.49 -6.84 -1.04 7.45 2.20 7.75 2.31 4.72 10.61 2.96 6.89 -3.03 2.57
Cytoplasmic Foci -0.04 8.67 9.70 37.78 6.19 -1.18 33.90 36.20 36.72 2.12 -4.50 35.41 41.57 53.89 7.38
Eisosomes 2.67 3.33 3.99 10.86 10.20 -1.15 7.77 17.37 11.79 9.82 -0.84 8.26 17.97 16.40 14.58
Endoplasmic Reticulum 1.18 -1.82 1.34 0.62 4.77 4.20 0.94 3.58 -1.31 4.91 3.73 -1.28 3.47 -0.72 6.29
Endosome -1.53 3.86 4.60 16.11 4.55 -10.34 12.12 14.03 19.92 4.41 -13.77 11.78 13.60 24.73 4.25
Golgi -5.69 5.03 1.69 7.58 -3.44 -8.25 13.59 6.26 11.83 -1.93 -9.99 13.85 4.66 12.52 -4.07
Lipid Particles 2.68 3.13 3.79 6.17 6.84 4.93 8.60 11.81 6.54 5.04 4.70 8.71 12.03 8.68 6.57
Mitochondria -0.33 2.22 1.09 2.09 -3.47 -2.03 5.19 2.70 4.98 -1.20 -1.65 5.38 2.71 5.29 -3.42
Mitotic Spindle -3.31 -2.39 -8.46 -5.53 -7.15 -4.86 0.10 -4.67 -1.76 -4.68 -4.91 -0.03 -8.59 -5.00 -8.64
None 1.08 0.40 -1.41 -5.43 -3.71 1.28 -1.46 2.28 0.02 2.31 2.18 -1.18 0.11 -2.39 1.28
Nuclear Periphery 1.05 -5.44 -4.80 -15.00 1.71 2.72 -7.76 -10.10 -12.76 2.30 2.88 -9.98 -14.49 -18.60 1.16
Nuclear Periphery Foci -2.15 -10.81 -4.98 -3.66 9.44 3.19 -10.60 -9.09 -9.77 4.00 -1.83 -13.41 -7.67 -6.99 8.19
Nucleolus -0.06 2.32 1.99 2.49 -1.95 1.01 4.58 11.39 6.66 6.76 -0.23 5.99 8.27 4.62 2.92
Nucleus 2.16 -2.75 -1.39 -17.74 3.14 19.54 -9.96 4.44 -9.55 12.01 20.27 -10.60 -0.44 -20.79 10.33
Peroxisomes -5.26 3.73 3.46 11.85 -2.41 -8.01 12.71 2.71 8.47 -2.08 -11.23 13.02 7.34 14.70 -3.13
Vacuole -0.80 -9.24 -13.54 -23.80 -0.15 -5.05 -19.43 -26.63 -24.46 -7.52 -5.84 -22.50 -37.67 -34.41 -5.14
Vacuole Periphery -0.83 4.87 2.26 4.01 -3.00 -3.15 6.31 1.56 3.15 -1.23 -3.43 6.86 2.20 5.38 -2.88
Standard name
Human Ortholog
Description PAS domain-containing serine/threonine protein kinase; coordinately regulates protein synthesis and carbohydrate metabolism and storage in response to a unknown metabolite that reflects nutritional status; PSK1 has a paralog, PSK2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (96%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Psk1

Psk1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Psk1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available