Standard name
Human Ortholog
Description Pre-ribosomal factor involved in 60S ribosomal protein subunit export; associates with the pre-60S particle; shuttles between the nucleus and cytoplasm
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0.13 0.15 0.09 0.14 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0.05 0.05 0.13 0.45 0.55 0.56 0.61 0.56 0.64 0.66 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.93 0.94 0.96 0.92 0.87 0.85 0.77 0.74 0.72 0.75 0.69 0.69 0.93 0.8 0.77 0.85 0.8 0.9 0.9 0.96 0.94 0.83 0.66 0.48
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0.06 0 0 0 0.07 0 0 0 0 0 0.05 0.07 0 0 0.05 0 0 0 0 0.08 0.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.2 0.33
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 0 0 0 0 2 4 13 7 4 6 9 1 1 0 0 0 0 5 0 0 3 2 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 1 1 12 9 7 18 25 14 22 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5 2 5 6 17 16 15 16 21 20 29 26 22 60 60 23 29 10 0 0 0 0 1 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 1 0 0 0 0 1 1 4 5 2 0 0 0 0 0 2
Endosome 2 0 1 0 0 0 0 0 0 0 0 0 0 1 3 0 0 0 4 0 0 3 3 6
Golgi 1 0 0 0 0 0 0 0 0 0 1 0 1 1 0 0 0 0 0 0 0 1 0 3
Mitochondria 13 3 15 17 63 263 390 394 340 317 342 506 0 2 0 7 4 1 3 0 1 1 3 9
Nucleus 255 132 312 294 422 498 545 518 399 427 367 522 348 364 315 213 172 188 251 136 300 268 213 191
Nuclear Periphery 0 0 4 9 11 7 14 16 5 16 8 10 2 5 4 0 1 1 0 0 0 0 0 3
Nucleolus 1 4 4 20 21 14 24 47 25 23 14 25 3 22 27 8 8 10 2 0 3 11 26 38
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 1 0 5 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 1 2 2 1 5 3 3 2 1 1 6 6 0 0 0 3 0 0 1 1 1
Vac/Vac Membrane 8 6 0 2 10 7 12 11 7 10 9 12 3 5 5 7 5 5 6 2 7 29 65 133
Unique Cell Count 275 140 324 318 483 584 708 704 558 571 531 761 376 455 407 251 214 208 281 143 319 324 322 402
Labelled Cell Count 286 147 341 350 547 821 1014 1027 826 845 792 1133 384 469 426 262 224 217 281 143 319 324 322 402


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.0 9.0 6.7 6.5 5.2 4.3 3.8 3.8 3.6 3.5 3.5 3.5 10.0 9.5 8.8 13.3 13.9 14.6 11.9 10.9 10.9
Std Deviation (1e-4) 1.9 1.8 1.2 1.4 1.4 1.1 0.9 1.0 1.0 0.8 0.9 0.8 2.4 2.6 2.3 4.3 5.4 4.7 2.3 2.1 1.9
Intensity Change (Log2) – – – -0.04 -0.36 -0.65 -0.83 -0.81 -0.88 -0.93 -0.95 -0.96 0.57 0.5 0.39 0.98 1.05 1.12 0.83 0.7 0.7


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.3 1.7 1.1 0.6 0.8 1.9 1.7 2.8 1.7 3.0 5.8 6.2 4.2 5.6 2.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.4 4.0 0 0 0 0 0 0 0 -4.2 -3.9 -4.4 -1.1 -1.7 -2.7
Nucleus -2.1 -4.3 -5.1 -7.7 -8.6 -9.0 -8.2 -9.5 -9.9 -2.1 -6.6 -7.3 -4.8 -6.0 -2.8
Nuclear Periphery 1.4 1.1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 3.4 2.5 0 0 0 0 0 0 0 0 2.8 3.6 1.6 0 2.5
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.2994 4.712 4.949 4.5069 4.1748 4.751 7.8045 6.9604 6.6245 5.8267 5.7665 6.7437 4.1642 4.7473 3.7604 4.6314 3.626 3.9078
Actin 0.0139 0 0.01 0.0059 0.0005 0.0009 0.0388 0 0.0061 0.0001 0.0004 0.0002 0.0267 0 0.005 0.0195 0.04 0.0026
Bud 0.0007 0 0.0002 0.0006 0 0.0001 0.0005 0 0.0001 0 0 0.0002 0.0005 0 0.0019 0.001 0.0031 0.0001
Bud Neck 0.0014 0.0001 0.0017 0.0009 0.0003 0.0009 0.0013 0.0003 0.0002 0 0.0004 0.0009 0.0003 0 0.0005 0.0005 0.0008 0.0013
Bud Periphery 0.0021 0 0.0007 0.0016 0.0001 0.0001 0.0017 0 0.0001 0 0.0001 0.0008 0.001 0 0.0015 0.0028 0.0052 0.0002
Bud Site 0.0016 0.0002 0.006 0.002 0.0001 0.0001 0.0017 0.0001 0.0006 0 0.0004 0.0005 0.006 0 0.0093 0.0011 0.0336 0.0002
Cell Periphery 0.0004 0 0.0009 0.0009 0 0 0.0005 0 0.0001 0 0 0.0003 0.0001 0 0.0002 0.0007 0.0007 0.0001
Cytoplasm 0.0041 0.0072 0.0086 0.0057 0.0001 0.0001 0.0148 0.0003 0.0008 0.0003 0.0044 0.0004 0.013 0 0.0039 0.0028 0.0008 0.0017
Cytoplasmic Foci 0.0179 0.0003 0.0058 0.0114 0.0011 0.001 0.0055 0 0.0018 0.0011 0.0095 0.0003 0.0074 0 0.0026 0.005 0.0009 0.0067
Eisosomes 0.0001 0 0.0001 0.0006 0 0 0.0012 0 0 0 0 0 0.0003 0 0 0.0004 0.0002 0
Endoplasmic Reticulum 0.0034 0.0003 0.0017 0.0078 0.0014 0.0002 0.0028 0.0001 0.0007 0 0.0025 0.0002 0.0011 0 0.0006 0.0064 0.0015 0.0002
Endosome 0.0159 0.0011 0.0071 0.0279 0.011 0.0039 0.0053 0.0001 0.005 0.0005 0.008 0.0022 0.0062 0 0.0014 0.031 0.002 0.0016
Golgi 0.0041 0 0.0035 0.0065 0.0028 0.0016 0.0029 0 0.0027 0.0001 0.0014 0.0002 0.0026 0 0.0008 0.0155 0.0008 0.0003
Lipid Particles 0.014 0.0001 0.0047 0.0155 0.0164 0.0023 0.0023 0 0.0035 0.0004 0.0271 0.0004 0.003 0 0.0009 0.0081 0.0008 0.0002
Mitochondria 0.0114 0.0002 0.0017 0.0098 0.001 0.0007 0.0131 0.0002 0.0005 0.0002 0.0007 0.0009 0.0061 0.0003 0.0007 0.0416 0.0077 0.0006
None 0.0036 0.0011 0.0015 0.01 0.0002 0.0003 0.0054 0 0.0006 0.0002 0.0022 0.0002 0.0117 0 0.0005 0.0053 0.0005 0.0003
Nuclear Periphery 0.0404 0.008 0.0183 0.0292 0.0144 0.0072 0.0326 0.0067 0.0024 0.0063 0.0092 0.0068 0.0034 0.0005 0.0026 0.0234 0.0098 0.0026
Nucleolus 0.0349 0.0309 0.0507 0.0428 0.0719 0.07 0.0202 0.0306 0.0455 0.0051 0.0243 0.0786 0.0068 0.0136 0.0165 0.0435 0.0366 0.052
Nucleus 0.7902 0.941 0.8592 0.7889 0.8746 0.9045 0.8338 0.9597 0.9269 0.9172 0.8676 0.8987 0.8894 0.9852 0.9465 0.7804 0.836 0.9229
Peroxisomes 0.0161 0 0.0024 0.0044 0.0003 0.001 0.0043 0 0.0005 0.0001 0.0231 0.0001 0.0026 0 0.0006 0.0018 0.0012 0.0022
Punctate Nuclear 0.0166 0.0062 0.0083 0.0113 0.0013 0.0044 0.0068 0.0011 0.0011 0.0682 0.0173 0.0029 0.0107 0.0001 0.0029 0.0037 0.0006 0.0031
Vacuole 0.0046 0.0028 0.0037 0.0108 0.0008 0.0003 0.0019 0.0004 0.0007 0 0.0009 0.0048 0.0005 0.0001 0.0006 0.0023 0.0102 0.0007
Vacuole Periphery 0.0026 0.0004 0.0032 0.0055 0.0014 0.0004 0.0026 0.0002 0.0002 0.0001 0.0004 0.0008 0.0004 0.0001 0.0002 0.0031 0.0071 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 57.8467 54.0269 49.2538 33.897 45.896 31.0646 31.9955 25.8084 21.17 35.4362
Translational Efficiency 2.0218 2.0813 1.6231 1.8919 2.0812 2.1825 1.6582 1.649 1.9226 1.6741

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2107 1544 1612 1246 1667 274 3025 45 3774 1818 4637 1291

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 726.90 1004.28 1071.34 1264.95 853.42 1030.28 1344.60 1614.53 782.78 1008.20 1249.60 1277.14
Standard Deviation 107.33 166.89 185.30 254.85 172.68 161.17 212.41 269.57 153.46 166.30 241.46 263.30
Intensity Change Log 2 — 0.466333 0.559588 0.799252 — 0.271709 0.655849 0.919786 — 0.364500 0.612368 0.865592

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000103 0.000381 0.000579 0.000857 0.000256 0.000586 0.000380 0.001293 0.000170 0.000412 0.000449 0.000872
Bud Neck 0.002523 0.008015 0.015375 0.034132 0.004534 0.003441 0.020093 0.033979 0.003411 0.007326 0.018453 0.034127
Bud Site 0.001210 0.000703 0.000744 0.003169 0.001054 0.000957 0.000795 0.007459 0.001141 0.000741 0.000777 0.003319
Cell Periphery 0.000563 0.000088 0.000073 0.000166 0.000294 0.000065 0.000173 0.000142 0.000444 0.000084 0.000138 0.000166
Cytoplasm 0.014124 0.000471 0.000317 0.003466 0.005882 0.000595 0.000143 0.003460 0.010484 0.000490 0.000204 0.003466
Cytoplasmic Foci 0.001420 0.000687 0.000111 0.001776 0.000557 0.005543 0.000009 0.001742 0.001039 0.001419 0.000044 0.001775
Eisosomes 0.000019 0.000029 0.000034 0.000038 0.000029 0.000030 0.000077 0.000053 0.000024 0.000030 0.000062 0.000039
Endoplasmic Reticulum 0.005303 0.006224 0.002709 0.002531 0.008125 0.009198 0.003225 0.002842 0.006549 0.006672 0.003046 0.002541
Endosome 0.000555 0.001394 0.000170 0.001680 0.001124 0.003212 0.000099 0.009984 0.000806 0.001668 0.000123 0.001970
Golgi 0.000037 0.000905 0.000431 0.001405 0.000243 0.003465 0.000022 0.003392 0.000128 0.001291 0.000164 0.001474
Lipid Particles 0.000228 0.000212 0.000017 0.000162 0.000292 0.001135 0.000007 0.000182 0.000256 0.000351 0.000011 0.000162
Mitochondria 0.000465 0.001013 0.001254 0.003665 0.001043 0.004211 0.000820 0.006063 0.000720 0.001495 0.000971 0.003749
Mitotic Spindle 0.000051 0.002121 0.000400 0.009927 0.002088 0.003130 0.000527 0.007267 0.000951 0.002273 0.000483 0.009834
None 0.017960 0.001226 0.000768 0.005276 0.017213 0.000579 0.000354 0.001231 0.017630 0.001128 0.000498 0.005135
Nuclear Periphery 0.000917 0.000600 0.000264 0.000937 0.000406 0.001078 0.000578 0.000352 0.000691 0.000672 0.000469 0.000917
Nuclear Periphery Foci 0.000285 0.000496 0.000028 0.000989 0.000742 0.000694 0.000094 0.000193 0.000487 0.000526 0.000071 0.000962
Nucleolus 0.008966 0.020204 0.010852 0.035616 0.017916 0.015668 0.006816 0.035901 0.012919 0.019520 0.008219 0.035626
Nucleus 0.943623* 0.952082* 0.964662* 0.886561* 0.936225* 0.935108* 0.964694* 0.868029* 0.940355* 0.949523* 0.964683* 0.885915*
Peroxisomes 0.000102 0.000482 0.000178 0.000544 0.000185 0.003551 0.000074 0.000836 0.000139 0.000945 0.000110 0.000554
Vacuole 0.001290 0.001818 0.000606 0.004013 0.001433 0.004573 0.000759 0.008049 0.001353 0.002233 0.000706 0.004154
Vacuole Periphery 0.000256 0.000849 0.000428 0.003089 0.000359 0.003179 0.000262 0.007551 0.000301 0.001201 0.000320 0.003245

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -13.95 -7.35 -17.28 -11.83 -5.54 -3.41 -6.56 -2.72 -2.18 -2.51 -10.64 -11.25 -15.93 -11.22 -10.25
Bud Neck -8.80 -13.20 -18.61 -15.42 -10.73 1.84 -16.37 -3.22 -3.32 -1.84 -7.23 -21.73 -18.49 -16.16 -9.99
Bud Site 1.16 0.98 -4.61 -6.73 -6.74 0.46 0.96 -1.27 -1.31 -1.34 1.45 1.29 -5.54 -6.82 -6.83
Cell Periphery 5.56 5.83 4.77 -2.10 -3.07 5.11 2.58 1.80 -0.98 0.34 6.85 5.84 5.36 -2.37 -0.53
Cytoplasm 9.38 9.50 6.28 -3.64 -3.83 5.12 5.69 1.40 -0.95 -1.17 10.73 11.08 5.62 -3.70 -4.06
Cytoplasmic Foci 1.82 3.76 0.24 -1.01 -2.07 -2.07 3.20 -0.50 1.70 -1.06 -0.64 4.82 -0.38 0.10 -2.26
Eisosomes -4.76 -9.65 -11.99 -4.52 -3.02 -0.16 -8.83 -4.05 -3.82 1.64 -2.88 -11.32 -9.50 -5.20 5.86
Endoplasmic Reticulum -1.75 5.40 5.02 11.29 -0.45 -0.57 10.68 2.84 2.26 -0.68 -0.26 10.41 9.99 9.87 0.82
Endosome -1.80 2.94 -1.90 0.25 -3.07 -1.80 3.60 -0.85 -0.50 -1.03 -1.92 4.77 -1.46 0.41 -3.30
Golgi -1.97 -0.99 -2.63 -0.18 -1.14 -2.01 2.00 -0.94 0.50 -1.05 -2.58 -0.19 -2.50 0.40 -2.31
Lipid Particles 0.24 4.69 1.06 0.43 -2.51 -1.79 3.09 -0.22 1.59 -1.76 -0.70 5.18 1.30 1.45 -2.82
Mitochondria -3.02 -3.50 -7.03 -5.36 -4.65 -1.67 0.63 -2.22 -0.43 -2.33 -2.35 -1.91 -6.57 -3.98 -6.06
Mitotic Spindle -2.81 -1.52 -5.54 -4.08 -5.29 -0.44 1.80 -0.76 -0.41 -1.26 -1.80 1.06 -5.07 -4.08 -5.35
None 9.29 9.58 6.36 -3.43 -3.90 7.48 7.58 6.76 -1.75 -2.14 11.71 12.22 7.52 -3.55 -4.22
Nuclear Periphery 1.48 3.58 -0.94 -3.22 -7.20 -1.02 -0.76 -0.67 0.86 0.33 0.07 1.18 -2.72 -2.38 -3.31
Nuclear Periphery Foci -0.59 2.45 -1.37 -0.74 -2.30 0.17 3.99 3.14 1.04 -0.74 -0.01 4.32 -0.80 -0.67 -2.18
Nucleolus -7.78 -2.59 -10.77 -6.91 -9.55 0.75 8.01 -1.82 -1.99 -2.89 -4.76 5.10 -9.71 -7.30 -11.08
Nucleus -1.95 -5.07 11.04 12.59 15.05 0.02 -6.57 2.22 2.09 3.11 -2.38 -8.32 11.29 12.33 15.94
Peroxisomes -2.04 -2.75 -5.60 -0.59 -4.60 -1.77 4.12 -3.80 1.42 -4.49 -2.49 1.31 -5.50 0.91 -5.79
Vacuole -0.87 2.41 -4.33 -3.16 -5.28 -1.97 2.49 -1.70 -0.96 -1.85 -1.54 3.34 -4.58 -3.00 -5.47
Vacuole Periphery -2.32 -0.83 -2.96 -1.95 -2.64 -2.13 1.48 -1.04 -0.37 -1.06 -3.09 -0.16 -3.07 -1.57 -3.04
Standard name
Human Ortholog
Description Pre-ribosomal factor involved in 60S ribosomal protein subunit export; associates with the pre-60S particle; shuttles between the nucleus and cytoplasm
Localization
Cell Percentages nucleus (96%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Ecm1

Ecm1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ecm1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available