Standard name | |
---|---|
Human Ortholog | |
Description | Type I transmembrane sorting receptor for multiple vacuolar hydrolases; cycles between the late-Golgi and prevacuolar endosome-like compartments |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 26 | 46 | 17 | 4 | 5 | 6 | 5 | 17 | 14 | 16 | 14 | 8 | 19 | 23 | 12 | – | – | – | 8 | 9 | 5 | 18 | 18 | 23 |
Bud | 1 | 2 | 5 | 4 | 0 | 10 | 18 | 33 | 12 | 23 | 9 | 17 | 4 | 4 | 2 | – | – | – | 2 | 0 | 0 | 2 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 3 | 1 | 0 | 1 | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 3 | 0 | 2 | 1 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 13 | 10 | 12 | 14 | 15 | 22 | 15 | 28 | 14 | 19 | 18 | 19 | 8 | 10 | 14 | – | – | – | 0 | 1 | 1 | 1 | 2 | 2 |
Endoplasmic Reticulum | 15 | 28 | 5 | 2 | 4 | 19 | 3 | 16 | 12 | 17 | 10 | 10 | 16 | 9 | 9 | – | – | – | 1 | 2 | 0 | 4 | 5 | 6 |
Endosome | 91 | 28 | 101 | 103 | 91 | 60 | 83 | 93 | 35 | 22 | 18 | 32 | 53 | 36 | 43 | – | – | – | 217 | 125 | 133 | 229 | 170 | 187 |
Golgi | 215 | 131 | 133 | 32 | 68 | 38 | 27 | 56 | 50 | 33 | 34 | 22 | 161 | 142 | 200 | – | – | – | 218 | 133 | 147 | 245 | 339 | 373 |
Mitochondria | 73 | 103 | 55 | 59 | 50 | 184 | 254 | 269 | 216 | 247 | 190 | 192 | 22 | 19 | 25 | – | – | – | 10 | 7 | 1 | 21 | 14 | 16 |
Nucleus | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | – | – | – | 0 | 1 | 1 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 1 | 5 | 0 | 1 | 2 | 2 | 4 | 2 | 5 | 2 | 2 | 2 | 5 | 0 | – | – | – | 0 | 1 | 1 | 0 | 2 | 0 |
Nucleolus | 15 | 7 | 4 | 24 | 15 | 19 | 18 | 20 | 14 | 18 | 6 | 14 | 7 | 6 | 9 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 6 | 5 | 1 | 1 | 7 | 7 | 3 | 5 | 3 | 1 | 1 | 2 | 0 | – | – | – | 2 | 1 | 7 | 11 | 6 | 7 |
SpindlePole | 5 | 0 | 5 | 9 | 4 | 3 | 9 | 7 | 6 | 12 | 1 | 5 | 4 | 2 | 3 | – | – | – | 1 | 1 | 1 | 2 | 0 | 1 |
Vac/Vac Membrane | 143 | 54 | 35 | 27 | 42 | 27 | 30 | 39 | 18 | 10 | 20 | 15 | 48 | 49 | 70 | – | – | – | 17 | 16 | 2 | 8 | 9 | 13 |
Unique Cell Count | 425 | 258 | 273 | 197 | 225 | 301 | 373 | 454 | 289 | 328 | 251 | 262 | 260 | 226 | 299 | 484 | 304 | 306 | 548 | 571 | 640 | |||
Labelled Cell Count | 600 | 410 | 383 | 283 | 296 | 392 | 473 | 592 | 400 | 430 | 326 | 342 | 345 | 310 | 388 | 484 | 304 | 306 | 548 | 571 | 640 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 12.7 | 13.1 | 11.3 | 10.9 | 10.3 | 9.2 | 8.7 | 8.9 | 8.5 | 7.9 | 8.2 | 7.8 | 12.5 | 12.6 | 12.9 | – | – | – | 11.5 | 13.3 | 14.3 |
Std Deviation (1e-4) | 2.0 | 2.3 | 1.9 | 2.0 | 1.8 | 1.5 | 1.6 | 1.6 | 1.8 | 1.6 | 1.6 | 1.5 | 2.0 | 2.3 | 2.3 | – | – | – | 2.4 | 2.8 | 3.4 |
Intensity Change (Log2) | – | – | – | -0.06 | -0.13 | -0.3 | -0.39 | -0.35 | -0.41 | -0.52 | -0.46 | -0.54 | 0.15 | 0.15 | 0.18 | – | – | – | 0.02 | 0.23 | 0.34 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -2.2 | -2.2 | -2.6 | -3.4 | -1.5 | -0.7 | -0.7 | -0.3 | -1.7 | 0.5 | 1.6 | -1.2 | – | – | – |
Bud | 0 | 0 | 1.1 | 2.0 | 3.2 | 1.6 | 3.0 | 1.2 | 2.7 | 0 | 0 | 0 | – | – | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cytoplasm | 1.3 | 1.1 | 1.5 | -0.2 | 1.0 | 0.3 | 0.8 | 1.4 | 1.4 | -0.8 | 0 | 0.2 | – | – | – |
Endoplasmic Reticulum | 0 | 0 | 2.7 | 0 | 1.3 | 1.6 | 2.2 | 1.5 | 1.4 | 2.6 | 1.4 | 0.9 | – | – | – |
Endosome | 3.3 | 0.8 | -4.5 | -4.1 | -4.9 | -6.9 | -9.2 | -8.1 | -6.6 | -4.2 | -5.2 | -6.2 | – | – | – |
Golgi | -7.3 | -4.2 | -9.4 | -12.1 | -10.8 | -7.9 | -10.6 | -8.6 | -10.3 | 3.1 | 3.2 | 4.4 | – | – | – |
Mitochondria | 2.4 | 0.6 | 9.9 | 12.1 | 10.3 | 12.9 | 13.5 | 12.7 | 12.3 | -3.8 | -3.7 | -4.1 | – | – | – |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nucleolus | 4.8 | 3.0 | 3.0 | 2.3 | 2.1 | 2.3 | 2.6 | 0 | 2.5 | 1.0 | 0 | 1.2 | – | – | – |
Peroxisomes | 0 | 0 | 0 | -0.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
SpindlePole | 1.7 | 0 | 0 | 0.5 | 0 | 0.2 | 1.3 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Vacuole | 0.3 | 1.8 | -1.5 | -2.0 | -1.8 | -2.7 | -4.5 | -1.8 | -2.8 | 1.8 | 2.6 | 3.3 | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.8111 | 10.3482 | 9.4416 | 8.7717 | 8.6794 | 9.3808 | 10.03 | 11.9234 | 11.4161 | 11.0919 | 10.4038 | 11.437 | 10.3809 | 11.6528 | 11.4132 | 11.3294 | 11.3148 | 11.6717 |
Actin | 0.0242 | 0.0132 | 0.0272 | 0.0453 | 0.0097 | 0.0042 | 0.0288 | 0.0008 | 0.0139 | 0.0469 | 0.0452 | 0.0026 | 0.0211 | 0.0111 | 0.0517 | 0.0749 | 0.0002 | 0.003 |
Bud | 0.0006 | 0.0004 | 0.0014 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0003 | 0.0004 | 0.0007 | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0003 | 0.0004 | 0 | 0 |
Bud Neck | 0.0007 | 0.0004 | 0.0011 | 0.0003 | 0.0003 | 0.0002 | 0.0003 | 0.0002 | 0.0003 | 0.0044 | 0.0007 | 0.0006 | 0.0003 | 0.0002 | 0.0006 | 0.0004 | 0.0001 | 0.0002 |
Bud Periphery | 0.0006 | 0.0004 | 0.0021 | 0.0002 | 0.0011 | 0.0001 | 0.0001 | 0.0002 | 0.0006 | 0.0019 | 0.0006 | 0 | 0.0001 | 0 | 0.0002 | 0.0006 | 0 | 0 |
Bud Site | 0.0024 | 0.0013 | 0.0068 | 0.0006 | 0.0007 | 0.0002 | 0.0005 | 0.0004 | 0.0004 | 0.0047 | 0.0009 | 0.0001 | 0.0003 | 0.0001 | 0.0029 | 0.0003 | 0 | 0.0001 |
Cell Periphery | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Cytoplasm | 0.0039 | 0.0066 | 0.0018 | 0.0017 | 0.001 | 0.0012 | 0.0063 | 0.0025 | 0.0054 | 0.0031 | 0.0041 | 0.0009 | 0.0019 | 0.0013 | 0.0164 | 0.001 | 0.0005 | 0.0009 |
Cytoplasmic Foci | 0.0691 | 0.0795 | 0.0575 | 0.0745 | 0.056 | 0.0427 | 0.0809 | 0.1033 | 0.083 | 0.0824 | 0.1381 | 0.0652 | 0.072 | 0.0589 | 0.0766 | 0.06 | 0.0764 | 0.0562 |
Eisosomes | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0002 | 0 | 0.0001 | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0003 | 0 | 0 |
Endoplasmic Reticulum | 0.0028 | 0.0011 | 0.0021 | 0.0004 | 0.0014 | 0.0007 | 0.0014 | 0.0009 | 0.0007 | 0.0008 | 0.0019 | 0.0005 | 0.0008 | 0.0006 | 0.0017 | 0.0008 | 0.0001 | 0.0005 |
Endosome | 0.2861 | 0.3904 | 0.2802 | 0.4096 | 0.3501 | 0.2363 | 0.3062 | 0.3664 | 0.2831 | 0.2669 | 0.3026 | 0.2188 | 0.2551 | 0.4417 | 0.3101 | 0.3138 | 0.3597 | 0.3007 |
Golgi | 0.4453 | 0.4524 | 0.5687 | 0.3302 | 0.4497 | 0.6423 | 0.518 | 0.4669 | 0.5693 | 0.4946 | 0.3974 | 0.6535 | 0.5594 | 0.4488 | 0.5119 | 0.518 | 0.556 | 0.6078 |
Lipid Particles | 0.0342 | 0.0205 | 0.009 | 0.0157 | 0.0515 | 0.0351 | 0.0304 | 0.0108 | 0.015 | 0.0092 | 0.0489 | 0.0321 | 0.0756 | 0.0082 | 0.0082 | 0.0104 | 0.004 | 0.0204 |
Mitochondria | 0.046 | 0.0044 | 0.0204 | 0.0582 | 0.031 | 0.0088 | 0.0039 | 0.0291 | 0.0113 | 0.0298 | 0.0061 | 0.0079 | 0.0058 | 0.0041 | 0.0041 | 0.0097 | 0.0002 | 0.0021 |
None | 0.0012 | 0.0001 | 0.0003 | 0.0006 | 0.0009 | 0.0006 | 0.0026 | 0.0001 | 0.0002 | 0.0051 | 0.0023 | 0.0016 | 0.0001 | 0 | 0.0006 | 0.0001 | 0 | 0 |
Nuclear Periphery | 0.0158 | 0.001 | 0.0079 | 0.0005 | 0.0164 | 0.0016 | 0.0032 | 0.0003 | 0.0007 | 0.0013 | 0.0031 | 0.0007 | 0.0002 | 0.0002 | 0.0021 | 0.0002 | 0 | 0.0002 |
Nucleolus | 0.0012 | 0.0001 | 0.0002 | 0.0003 | 0.0023 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0105 | 0.0004 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.0112 | 0.0002 | 0.0007 | 0.0003 | 0.0025 | 0.0002 | 0.0041 | 0.0002 | 0.0002 | 0.0055 | 0.0003 | 0.0002 | 0.0001 | 0 | 0.0014 | 0 | 0 | 0 |
Peroxisomes | 0.0309 | 0.0024 | 0.0028 | 0.0202 | 0.0049 | 0.015 | 0.0077 | 0.0049 | 0.0108 | 0.0187 | 0.0282 | 0.0077 | 0.0023 | 0.0136 | 0.0037 | 0.0042 | 0.0005 | 0.0014 |
Punctate Nuclear | 0.0049 | 0.0002 | 0.0008 | 0.0189 | 0.0036 | 0.0026 | 0.0013 | 0.0001 | 0.0003 | 0.0036 | 0.0099 | 0.003 | 0.0001 | 0.0001 | 0.0014 | 0.0001 | 0 | 0.0001 |
Vacuole | 0.0085 | 0.0166 | 0.004 | 0.0133 | 0.0054 | 0.0024 | 0.0014 | 0.0056 | 0.0018 | 0.006 | 0.0047 | 0.0014 | 0.0013 | 0.0044 | 0.0024 | 0.0017 | 0.0006 | 0.0024 |
Vacuole Periphery | 0.0099 | 0.0087 | 0.0048 | 0.0089 | 0.0105 | 0.0055 | 0.0024 | 0.0065 | 0.0023 | 0.0026 | 0.0039 | 0.0028 | 0.0031 | 0.0065 | 0.0035 | 0.0031 | 0.0016 | 0.0041 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 56.9161 | 47.8621 | 46.0161 | 57.3559 | 47.838 | 54.4417 | 60.5969 | 66.4606 | 63.3794 | 58.6625 |
Translational Efficiency | 0.7619 | 0.7271 | 0.8195 | 0.6329 | 0.5748 | 0.8623 | 0.7256 | 0.5923 | 0.5946 | 0.6084 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
148 | 1119 | 198 | 501 | 487 | 2534 | 2107 | 2249 | 635 | 3653 | 2305 | 2750 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1187.25 | 1133.51 | 1651.78 | 1375.12 | 1133.15 | 1136.98 | 1430.15 | 1385.46 | 1145.76 | 1135.92 | 1449.19 | 1383.58 |
Standard Deviation | 161.58 | 185.39 | 252.29 | 226.06 | 1343.57 | 169.67 | 207.71 | 205.59 | 1179.43 | 174.64 | 220.83 | 209.51 |
Intensity Change Log 2 | — | -0.066827 | 0.476398 | 0.211934 | — | 0.004868 | 0.335828 | 0.290026 | — | -0.031370 | 0.409461 | 0.250598 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001102 | 0.004608 | 0.002388 | 0.007901 | 0.003596 | 0.001119 | 0.001142 | 0.003738 | 0.003014 | 0.002188 | 0.001249 | 0.004497 |
Bud Neck | 0.001325 | 0.000308 | 0.007411 | 0.001338 | 0.001960 | 0.000078 | 0.000824 | 0.000300 | 0.001812 | 0.000148 | 0.001390 | 0.000489 |
Bud Site | 0.012842 | 0.002150 | 0.002540 | 0.007296 | 0.014874 | 0.000565 | 0.001434 | 0.001816 | 0.014400 | 0.001051 | 0.001529 | 0.002815 |
Cell Periphery | 0.000273 | 0.000117 | 0.000290 | 0.000281 | 0.000996 | 0.000074 | 0.000229 | 0.000153 | 0.000827 | 0.000087 | 0.000234 | 0.000176 |
Cytoplasm | 0.002543 | 0.000718 | 0.000306 | 0.002063 | 0.019016 | 0.000178 | 0.000316 | 0.000428 | 0.015176 | 0.000344 | 0.000315 | 0.000726 |
Cytoplasmic Foci | 0.094967 | 0.117660 | 0.094500 | 0.074980 | 0.108401 | 0.093757 | 0.065367 | 0.055049 | 0.105270 | 0.101079 | 0.067870 | 0.058680 |
Eisosomes | 0.000018 | 0.000053 | 0.000103 | 0.000056 | 0.000097 | 0.000031 | 0.000041 | 0.000051 | 0.000078 | 0.000037 | 0.000047 | 0.000052 |
Endoplasmic Reticulum | 0.002663 | 0.000563 | 0.001020 | 0.000730 | 0.002210 | 0.000250 | 0.000304 | 0.000371 | 0.002315 | 0.000346 | 0.000365 | 0.000436 |
Endosome | 0.308384* | 0.266145* | 0.148828 | 0.228763* | 0.265792* | 0.351183* | 0.302859* | 0.245946* | 0.275719* | 0.325134* | 0.289628* | 0.242816* |
Golgi | 0.308068* | 0.552044* | 0.390268* | 0.546877* | 0.434785* | 0.496666* | 0.344886* | 0.607158* | 0.405251* | 0.513630* | 0.348785* | 0.596176* |
Lipid Particles | 0.002259 | 0.015609 | 0.137879* | 0.020664 | 0.014979 | 0.007206 | 0.068902 | 0.013756 | 0.012014 | 0.009780 | 0.074827 | 0.015014 |
Mitochondria | 0.007281 | 0.010083 | 0.011635 | 0.005495 | 0.024013 | 0.013882 | 0.028310 | 0.011978 | 0.020113 | 0.012718 | 0.026878 | 0.010797 |
Mitotic Spindle | 0.001728 | 0.001562 | 0.007641 | 0.002426 | 0.000785 | 0.000128 | 0.001335 | 0.000700 | 0.001005 | 0.000567 | 0.001877 | 0.001014 |
None | 0.000040 | 0.000026 | 0.000129 | 0.000041 | 0.006965 | 0.000045 | 0.000079 | 0.000034 | 0.005351 | 0.000039 | 0.000084 | 0.000035 |
Nuclear Periphery | 0.000211 | 0.000074 | 0.008385 | 0.000433 | 0.000130 | 0.000072 | 0.002291 | 0.000165 | 0.000149 | 0.000073 | 0.002815 | 0.000214 |
Nuclear Periphery Foci | 0.001093 | 0.000292 | 0.009925 | 0.002863 | 0.000963 | 0.000222 | 0.004573 | 0.000454 | 0.000994 | 0.000243 | 0.005032 | 0.000893 |
Nucleolus | 0.000030 | 0.000008 | 0.000493 | 0.000033 | 0.000477 | 0.000175 | 0.000074 | 0.000014 | 0.000373 | 0.000124 | 0.000110 | 0.000017 |
Nucleus | 0.000039 | 0.000028 | 0.000118 | 0.000026 | 0.025585 | 0.000066 | 0.000022 | 0.000011 | 0.019631 | 0.000054 | 0.000030 | 0.000013 |
Peroxisomes | 0.008727 | 0.001996 | 0.003382 | 0.002808 | 0.002842 | 0.000801 | 0.001585 | 0.000810 | 0.004214 | 0.001167 | 0.001740 | 0.001174 |
Vacuole | 0.165410 | 0.013770 | 0.120641 | 0.067479 | 0.036015 | 0.014855 | 0.103652 | 0.031569 | 0.066173 | 0.014523 | 0.105111 | 0.038111 |
Vacuole Periphery | 0.080997 | 0.012186 | 0.052117 | 0.027449 | 0.035520 | 0.018649 | 0.071774 | 0.025499 | 0.046119 | 0.016669 | 0.070085 | 0.025854 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.10 | -1.82 | -2.60 | -1.19 | -2.08 | 2.10 | 2.02 | -0.08 | -3.24 | -3.01 | 0.88 | 1.84 | -1.22 | -2.66 | -3.77 |
Bud Neck | 2.62 | -4.67 | -0.06 | -4.86 | 4.80 | 4.30 | 2.94 | 3.89 | -6.32 | 7.44 | 4.70 | 1.77 | 3.90 | -6.60 | 6.65 |
Bud Site | 1.98 | 1.88 | 0.93 | -3.33 | -2.97 | 5.34 | 5.02 | 4.89 | -4.68 | -1.07 | 5.58 | 5.37 | 4.82 | -4.70 | -3.12 |
Cell Periphery | 1.59 | -0.20 | -0.11 | -2.86 | 0.12 | 5.16 | 4.23 | 4.72 | -5.20 | 1.66 | 5.31 | 4.16 | 4.66 | -5.44 | 1.34 |
Cytoplasm | 2.24 | 2.88 | -0.15 | -1.78 | -2.21 | 5.51 | 5.48 | 5.44 | -3.65 | -1.61 | 5.60 | 5.61 | 5.42 | -2.28 | -2.62 |
Cytoplasmic Foci | -2.06 | 0.04 | 1.78 | 8.24 | 2.29 | 2.40 | 6.94 | 8.76 | 14.90 | 3.95 | 0.82 | 6.83 | 8.72 | 18.26 | 3.67 |
Eisosomes | -5.89 | -6.46 | -3.86 | -0.29 | 3.07 | 2.43 | 2.02 | 1.68 | -4.66 | -1.74 | 1.96 | 1.50 | 1.27 | -3.55 | -0.99 |
Endoplasmic Reticulum | 1.04 | 0.81 | 0.95 | -1.27 | 1.30 | 3.27 | 3.18 | 3.07 | -2.75 | -1.52 | 3.01 | 2.97 | 2.87 | -2.06 | -1.52 |
Endosome | 1.91 | 6.77 | 3.46 | 3.42 | -5.81 | -7.48 | -3.32 | 1.37 | 15.63 | 7.82 | -4.98 | -1.50 | 2.84 | 14.35 | 7.00 |
Golgi | -10.57 | -2.65 | -9.38 | 0.42 | -6.27 | -4.60 | 5.61 | -11.85 | -14.51 | -32.15* | -8.92 | 3.96 | -14.91 | -12.24 | -32.16* |
Lipid Particles | -5.84 | -8.39 | -5.68 | -1.35 | 7.12 | 2.32 | -11.53 | 0.34 | -4.12 | 15.16 | 0.84 | -14.87 | -1.08 | -3.44 | 16.49 |
Mitochondria | -0.67 | -0.93 | 0.41 | 1.77 | 1.83 | 2.42 | -0.97 | 2.90 | 1.14 | 6.98 | 2.20 | -1.83 | 2.78 | 1.37 | 7.52 |
Mitotic Spindle | 0.14 | -1.60 | -0.97 | -0.94 | 1.43 | 2.98 | -0.61 | 1.03 | -3.21 | 1.89 | 1.87 | -1.42 | 0.63 | -1.57 | 1.94 |
None | 0.94 | -3.95 | -0.11 | -1.97 | 4.64 | 2.73 | 2.72 | 2.73 | 0.39 | 7.28 | 2.73 | 2.71 | 2.73 | 0.21 | 8.53 |
Nuclear Periphery | 2.97 | -2.73 | -1.77 | -3.01 | 2.65 | 3.24 | -8.43 | -0.63 | -5.14 | 8.38 | 4.28 | -7.62 | -1.60 | -5.35 | 7.46 |
Nuclear Periphery Foci | 3.56 | -3.49 | -2.07 | -3.08 | 2.65 | 2.84 | -6.05 | 2.02 | -3.54 | 8.12 | 3.55 | -7.11 | 0.59 | -4.01 | 7.74 |
Nucleolus | 2.93 | -3.61 | -0.29 | -2.33 | 3.57 | 1.66 | 2.35 | 2.56 | 0.94 | 6.82 | 1.77 | 2.13 | 2.56 | 0.90 | 6.63 |
Nucleus | 0.37 | -2.54 | 0.32 | -0.13 | 2.67 | 4.61 | 4.62 | 4.62 | 0.89 | 2.93 | 4.59 | 4.60 | 4.60 | 0.92 | 3.25 |
Peroxisomes | 1.64 | 1.30 | 1.45 | -0.94 | 0.63 | 2.57 | 1.64 | 2.63 | -0.03 | 4.02 | 2.69 | 2.23 | 2.73 | -0.02 | 2.77 |
Vacuole | 9.00 | 2.19 | 5.55 | -10.18 | 4.12 | 5.47 | -13.30 | 1.23 | -9.25 | 20.55 | 9.62 | -6.21 | 5.07 | -13.32 | 19.48 |
Vacuole Periphery | 7.12 | 2.54 | 5.28 | -4.46 | 3.51 | 4.15 | -8.16 | 2.37 | -4.59 | 17.96* | 7.56 | -5.56 | 5.03 | -6.86 | 18.13* |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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