Standard name
Human Ortholog
Description ATP-dependent Lon protease; involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.1 0 0.05 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0.1 0.07 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.09 0.16 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.1 0 0.07 0 0.05 0 0 0 – 0 0 0 0.09 0.09 0.06 0.08 0.12 0.08 0.05 0.1 0.05 0.06 0.09 0.07
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0.26 0.26 0.29 0 0 0.08 0.05 0.08 0.06 0.07 0.08 0.12
Mitochondria 0.75 0.81 0.7 0.82 0.78 0.86 0.87 0.88 – 0.92 0.95 0.92 0.62 0.62 0.6 0.09 0.09 0.08 0.71 0.53 0.58 0.6 0.53 0.54
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0.05 0.06 0.08 0.06 – 0.09 0.1 0.1 0 0 0 0.08 0.05 0.06 0 0 0 0 0 0
Nucleolus 0.11 0.07 0.06 0 0.11 0.13 0.15 0.12 – 0.13 0.08 0.12 0 0 0 0.12 0.1 0.13 0 0 0 0 0 0
Peroxisomes 0.13 0.14 0.34 0.2 0.13 0.06 0.06 0 – 0 0 0 0.07 0.06 0.09 0.34 0.25 0.3 0.07 0.09 0.19 0.13 0.15 0.14
SpindlePole 0 0 0.08 0 0 0 0 0 – 0 0 0 0.06 0.06 0 0.11 0.11 0.07 0 0 0 0.05 0 0
Vac/Vac Membrane 0.12 0.08 0 0 0 0 0 0 – 0 0 0 0.2 0.2 0.19 0.13 0.09 0.14 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 0 – 1 1 2 4 4 2 0 1 0 11 14 9 5 11 13
Bud 12 12 12 7 12 12 12 17 – 18 12 9 4 4 1 19 6 7 3 8 2 4 2 1
Bud Neck 1 1 0 0 0 0 0 0 – 1 0 0 1 1 0 0 0 0 1 3 0 2 5 2
Bud Site 1 1 4 0 1 5 4 6 – 6 7 4 0 0 0 6 6 1 – – – – – –
Cell Periphery 8 5 4 2 2 0 4 1 – 2 5 3 3 3 3 9 14 9 0 0 0 0 0 0
Cytoplasm 2 4 10 6 8 8 3 7 – 3 2 1 6 6 11 17 22 8 0 2 0 1 0 0
Endoplasmic Reticulum 0 2 1 0 1 1 1 1 – 4 1 3 0 0 1 4 2 1 0 0 0 0 0 0
Endosome 48 6 24 3 14 3 7 6 – 1 0 2 20 19 15 15 17 11 28 30 20 16 29 23
Golgi 5 0 1 2 5 1 0 2 – 0 0 2 55 55 72 4 6 11 27 24 21 18 24 41
Mitochondria 370 220 240 200 218 351 429 543 – 465 342 428 133 133 148 17 12 11 384 159 215 163 162 195
Nucleus 2 3 1 1 0 2 0 4 – 0 1 1 2 2 1 3 9 4 0 0 0 0 0 0
Nuclear Periphery 16 10 11 6 14 26 37 34 – 43 36 46 5 6 5 15 7 8 1 0 2 1 3 3
Nucleolus 55 20 19 11 32 52 73 73 – 64 30 57 8 8 6 24 14 17 4 3 2 2 3 2
Peroxisomes 66 38 119 49 36 24 31 20 – 11 6 14 16 13 22 68 35 40 37 26 71 34 44 50
SpindlePole 18 8 28 10 8 8 8 17 – 4 7 10 13 13 4 22 15 9 17 11 12 13 12 12
Vac/Vac Membrane 57 23 10 6 8 12 12 11 – 18 5 6 42 43 47 26 12 19 12 11 3 5 3 5
Unique Cell Count 491 271 345 245 279 409 492 618 505 359 466 214 215 247 198 141 132 542 301 371 274 307 361
Labelled Cell Count 661 354 484 303 359 505 621 742 641 455 588 312 310 338 249 178 156 542 301 371 274 307 361


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.0 9.5 8.5 9.2 10.1 9.6 9.3 8.6 – 8.1 7.7 8.9 10.5 10.5 10.7 8.0 8.6 11.2 10.2 11.2 11.6
Std Deviation (1e-4) 1.9 2.0 2.1 3.2 2.7 2.9 3.3 2.6 – 2.6 2.4 3.0 1.9 1.9 2.3 1.9 3.0 3.2 2.6 2.6 2.3
Intensity Change (Log2) – – – 0.12 0.24 0.17 0.13 0.02 – -0.06 -0.14 0.07 0.31 0.31 0.34 -0.08 0.02 0.4 0.27 0.4 0.45


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud -0.4 0.5 -0.4 -0.9 -0.6 – 0.1 -0.1 -1.4 -1.1 -1.1 -2.5 3.0 0.4 0.9
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0.8 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 4.6 0
Cytoplasm -0.3 0 -0.8 -2.6 -2.0 – -2.7 -2.4 0 -0.1 -0.1 1.0 2.9 5.1 0
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Endosome -3.3 -1.0 -4.6 -4.2 -5.1 – -5.7 -5.1 -5.2 1.0 0.8 -0.4 0.3 1.8 0.5
Golgi 0 0 0 0 0 – 0 0 0 9.7 9.7 10.5 0 0 0
Mitochondria 3.3 2.4 5.4 6.3 7.0 – 8.6 9.0 8.2 -1.8 -1.9 -2.4 -13.7 -12.2 -12.0
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery -0.5 1.2 2.0 2.7 1.6 – 3.1 3.6 3.7 -0.6 -0.3 -0.9 2.3 0.9 1.4
Nucleolus -0.6 2.7 3.4 4.2 3.2 – 3.5 1.5 3.2 -0.9 -1.0 -1.8 2.7 1.8 2.7
Peroxisomes -3.8 -6.2 -10.0 -10.5 -13.2 – -12.9 -11.4 -12.0 -7.3 -7.7 -7.2 0 -2.1 -0.9
SpindlePole -2.0 -2.8 -4.0 -4.6 -3.8 – -5.5 -3.8 -4.0 -0.9 -0.9 -3.4 1.2 0.9 -0.5
Vacuole -0.3 0 0 -0.4 -1.1 – 0.5 -1.4 -1.6 6.6 6.7 6.6 4.6 2.7 4.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.959 10.6705 10.2788 10.1007 8.9543 10.412 9.5216 13.3129 11.9999 11.8646 12.8823 12.9345 3.8127 12.8043 12.3139 10.6606 12.0899 12.5316
Actin 0.0188 0.0048 0.0227 0.0101 0.0007 0.0011 0.0024 0.0013 0.0159 0.0185 0.0291 0.009 0.0017 0.01 0.0045 0.0189 0.0007 0.0006
Bud 0.0002 0.001 0.0001 0.0016 0 0.0001 0.0003 0 0.0001 0.0002 0.0004 0 0.0005 0.0007 0.0002 0.0008 0.0062 0
Bud Neck 0.0004 0.0001 0.0001 0.001 0 0.0001 0.0008 0.0001 0.0001 0.0008 0.0002 0 0.0008 0.0011 0.0013 0.0004 0 0
Bud Periphery 0.0003 0.001 0.0003 0.0012 0.0002 0.0002 0.0002 0.0001 0.0003 0.0006 0.0004 0.0001 0.0011 0.0007 0.0002 0.001 0.0337 0.0003
Bud Site 0.0005 0.0052 0.0002 0.0083 0 0.0001 0.0126 0.0001 0.0003 0.0007 0.0052 0.0001 0.0019 0.026 0.0035 0.001 0.0006 0
Cell Periphery 0.0002 0.0006 0.0003 0.0002 0.0002 0.0001 0.0002 0.0005 0.0001 0.0002 0.0001 0 0.0012 0.0011 0.0002 0.0001 0.0006 0.0001
Cytoplasm 0.0023 0.0007 0 0.0038 0.0004 0 0.0043 0 0 0.0001 0.0001 0 0.0273 0.0003 0.0001 0.0002 0 0
Cytoplasmic Foci 0.009 0.0021 0.0007 0.0026 0.0009 0.0006 0.0135 0.001 0.0053 0.0074 0.0061 0.0004 0.0065 0.0211 0.0149 0.0068 0.0002 0.0002
Eisosomes 0.0008 0.0003 0.0002 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0004 0.0002 0.0001 0.0002 0.0011 0.0001 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0014 0.0001 0.0001 0.0008 0.0038 0.0001 0.002 0 0 0.0001 0 0.0003 0.0083 0.0001 0.0001 0.0002 0.0002 0.0002
Endosome 0.0099 0.0053 0.0023 0.0033 0.0105 0.0049 0.0183 0.0017 0.0016 0.0056 0.0009 0.0013 0.084 0.0058 0.0084 0.0143 0.0009 0.0013
Golgi 0.0092 0.0121 0.0098 0.0063 0.008 0.008 0.005 0.0033 0.0037 0.0126 0.0039 0.0083 0.0322 0.0075 0.0131 0.0437 0.0003 0.0025
Lipid Particles 0.024 0.0199 0.0056 0.002 0.0153 0.0047 0.0469 0.017 0.012 0.0449 0.0018 0.0025 0.0361 0.0141 0.0398 0.0029 0.0005 0.0125
Mitochondria 0.8567 0.919 0.9333 0.937 0.9267 0.9723 0.8555 0.9039 0.9411 0.8721 0.927 0.9744 0.1393 0.8803 0.8884 0.9055 0.9455 0.9712
None 0.0043 0.0001 0 0.0007 0.0003 0 0.0077 0 0 0.0002 0 0.0001 0.0046 0.0002 0 0 0.0002 0
Nuclear Periphery 0.0142 0.001 0.0103 0.0027 0.0182 0.0002 0.004 0.0001 0 0.0001 0.0001 0.0001 0.1575 0.0001 0.0001 0.0001 0.0002 0.0004
Nucleolus 0.0028 0.0005 0.0001 0.0006 0.0002 0.0005 0.0016 0.0033 0.0001 0.0003 0 0.0002 0.0617 0.0005 0.0003 0 0.0005 0
Nucleus 0.0184 0.0002 0.0001 0.0055 0.0009 0.0003 0.0008 0.0005 0.0001 0.0001 0 0.0001 0.2185 0.0002 0.0001 0.0001 0.0004 0
Peroxisomes 0.0065 0.0053 0.0024 0.0019 0.0008 0.0012 0.0154 0.0398 0.0175 0.025 0.0236 0.0013 0.0011 0.0263 0.0144 0.0022 0.0005 0.007
Punctate Nuclear 0.0082 0 0 0.0056 0.0002 0.0001 0.0009 0 0.0001 0.0003 0 0 0.1607 0.0011 0.0002 0.0001 0 0
Vacuole 0.0027 0.0057 0.0019 0.0009 0.0043 0.0014 0.0036 0.0112 0.0004 0.0018 0.0002 0.0002 0.0306 0.0009 0.0023 0.0007 0.0039 0.0005
Vacuole Periphery 0.009 0.0149 0.0093 0.004 0.0083 0.004 0.0038 0.016 0.0012 0.0081 0.0007 0.0015 0.024 0.0011 0.0076 0.0008 0.005 0.0031

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 93.5721 72.485 79.8199 86.0914 62.0268 90.4407 85.9686 93.7866 98.4797 82.4413
Translational Efficiency 1.008 1.0132 0.9415 0.9483 0.9995 1.1568 0.9788 0.9435 0.9437 0.9362

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description ATP-dependent Lon protease; involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria
Localization
Cell Percentages mitochondrion (89%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Pim1

Pim1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pim1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available