Standard name
Human Ortholog
Description B subunit of DNA polymerase alpha-primase complex; required for initiation of DNA replication during mitotic and premeiotic DNA synthesis; also functions in telomere capping and length regulation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 – 0 0 0 0.11 0.12 0.11 0 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.05 0 0.13 0.12 0.09 0.34 – 0.42 0.18 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.86 0.91 0.93 0.93 0.9 0.87 0.85 0.87 0.84 – 0.74 0.84 0.93 0.86 0.83 0.63 0.73 0.7 0.78 0.85 0.84 0.74 0.73 0.62
Nuclear Periphery 0.07 0.06 0 0 0.07 0.05 0.06 0 0 – 0 0 0 0 0 0.06 0.06 0.07 0.07 0 0 0 0 0
Nucleolus 0.08 0.07 0 0 0 0 0 0 0.06 – 0.1 0.07 0.07 0.07 0.07 0 0 0 0.05 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0.06 0.06 0 0.07 0 0.05 0 0 – 0 0 0 0 0 0.23 0.21 0.2 0.05 0.06 0.05 0.07 0.19 0.19
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 1 1 0 0 0
Bud 0 0 0 0 2 0 2 8 5 – 8 6 0 0 0 0 0 0 4 3 0 0 0 2
Bud Neck 0 0 0 0 0 0 1 0 1 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 1 3 8 6 5 13 – 5 12 0 0 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 2 2 0 0 0 0 0 0 0
Cytoplasm 9 7 8 10 16 11 20 18 8 – 8 15 3 18 28 49 9 23 0 0 0 0 0 0
Endoplasmic Reticulum 0 1 1 0 0 0 3 0 0 – 0 0 0 0 0 13 7 9 0 0 0 0 1 2
Endosome 1 1 2 0 0 1 0 0 0 – 0 0 0 0 0 3 1 2 2 0 3 0 0 3
Golgi 0 0 2 0 0 0 0 0 0 – 0 0 0 0 0 2 1 5 1 0 3 0 0 1
Mitochondria 9 12 3 15 9 67 89 52 195 – 244 134 5 1 4 6 2 5 2 2 7 0 1 3
Nucleus 223 304 410 297 423 438 625 528 481 – 434 617 112 145 188 287 199 240 208 295 379 28 107 98
Nuclear Periphery 17 19 9 13 35 25 44 24 24 – 17 22 5 1 3 29 17 25 18 8 18 1 0 1
Nucleolus 21 22 5 8 6 22 26 19 33 – 57 53 8 11 17 13 7 3 13 10 12 0 2 6
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0
SpindlePole 1 0 3 1 4 5 3 9 2 – 1 6 0 0 3 3 1 1 2 1 1 0 0 2
Vac/Vac Membrane 17 21 25 7 31 19 38 20 16 – 18 22 1 2 2 105 58 68 12 19 21 2 28 30
Unique Cell Count 259 335 441 318 471 501 738 610 572 583 738 120 168 227 458 273 341 268 346 453 38 148 160
Labelled Cell Count 298 387 469 352 529 596 857 683 778 792 887 134 178 246 513 304 381 268 346 453 38 148 160


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.2 7.1 6.6 5.9 6.5 5.2 5.6 5.9 4.7 – 4.5 5.2 6.6 7.8 6.6 9.6 9.5 9.2 – 5.8 6.1
Std Deviation (1e-4) 1.1 1.7 1.0 1.1 1.1 1.0 1.2 1.1 1.0 – 1.0 0.9 1.5 8.7 1.9 2.2 2.0 2.2 – 1.3 1.4
Intensity Change (Log2) – – – -0.16 -0.02 -0.34 -0.25 -0.16 -0.5 – -0.55 -0.34 0.01 0.25 -0.01 0.54 0.53 0.49 – -0.19 -0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 1.2 1.5 0.4 1.0 1.2 -0.5 – -0.6 0.3 0 4.9 5.7 5.5 1.3 3.5
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 3.2 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 3.6 1.6 7.4 7.0 5.6 0 – 0 9.1 0 0 0 0 0 0
Nucleus 0.2 -1.7 -2.8 -4.2 -3.3 -4.3 – -7.7 -4.6 0.1 -2.6 -4.1 -10.9 -7.4 -8.4
Nuclear Periphery 1.7 3.8 2.4 3.1 1.7 0 – 0 1.0 0 0 0 3.2 2.9 3.6
Nucleolus 1.4 0.2 3.0 2.5 2.1 0 – 0 4.6 0 0 4.4 1.8 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1.2 0 – 0 0 0 0 0 0 0 0
Vacuole -2.3 0.6 -1.4 -0.4 -1.9 -2.3 – -2.0 -2.3 -2.2 -2.4 -3.0 7.4 6.3 6.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.6966 3.363 2.6133 2.18 1.6012 2.0298 3.2808 4.9721 4.5269 3.9756 3.5257 3.4903 3.395 4.7917 4.0949 3.6903 3.0112 3.7774
Actin 0.0047 0.0011 0.008 0.0005 0.0186 0.0001 0.0055 0 0.0049 0.0008 0.001 0.0002 0.0084 0 0.0124 0.0849 0.0011 0.0055
Bud 0.0001 0 0 0 0.0001 0 0.0003 0 0.0005 0.0001 0.0003 0 0.0002 0 0.0004 0.002 0.0004 0
Bud Neck 0.0003 0.0001 0.0001 0.0001 0.0005 0.0005 0.0011 0.0001 0.0005 0.0004 0.0016 0.0008 0.0007 0 0.0007 0.0016 0.0008 0.0004
Bud Periphery 0.0002 0.0001 0.0001 0.0001 0.0003 0.0001 0.0009 0 0.0008 0.0002 0.0008 0.0002 0.0005 0 0.0012 0.0032 0.0016 0.0001
Bud Site 0.0002 0.0002 0 0 0.0003 0 0.0009 0 0.0063 0.0002 0.0012 0.0001 0.0005 0 0.0009 0.0197 0.0003 0
Cell Periphery 0.0001 0 0 0 0.0001 0 0.0004 0 0.0002 0.0001 0.0005 0.0001 0.0001 0 0.0002 0.0009 0.0007 0
Cytoplasm 0.0034 0.0002 0.0001 0.0014 0.0013 0.0002 0.0019 0 0.0013 0.0015 0.0075 0.0002 0.0025 0.0001 0.0003 0.0159 0.0015 0.0001
Cytoplasmic Foci 0.005 0.0019 0 0 0.0038 0 0.0057 0 0.0069 0.0026 0.0017 0.0004 0.0108 0 0.003 0.026 0.0081 0.0004
Eisosomes 0.0002 0 0.0001 0 0.0002 0 0.0001 0 0 0 0.0001 0 0 0 0.0001 0.0008 0 0
Endoplasmic Reticulum 0.0018 0.0001 0.0004 0.001 0.003 0.0004 0.0027 0 0.0005 0.0003 0.0066 0.0009 0.0016 0.0002 0.0005 0.0072 0.0013 0.0003
Endosome 0.006 0.0003 0.0003 0.0005 0.0141 0.0001 0.0082 0 0.009 0.0027 0.0267 0.004 0.0147 0 0.006 0.0571 0.0019 0.0002
Golgi 0.0036 0.0007 0.0004 0 0.0032 0 0.0061 0 0.0047 0.0038 0.0038 0.0007 0.0026 0 0.0045 0.0198 0.0004 0.0002
Lipid Particles 0.0105 0.0024 0 0.0002 0.007 0 0.01 0 0.0078 0.0231 0.0046 0.001 0.0124 0 0.0061 0.0602 0.0014 0.0003
Mitochondria 0.0125 0.0007 0.0004 0.0009 0.0019 0.0004 0.0208 0.0001 0.0086 0.0032 0.0076 0.0011 0.0021 0.0003 0.0054 0.0309 0.0118 0.0011
None 0.0163 0.0001 0 0.0032 0.0052 0 0.0031 0 0.0005 0.001 0.0006 0.0002 0.0022 0 0.0002 0.0054 0.0012 0.0001
Nuclear Periphery 0.0688 0.0473 0.0619 0.3508 0.4746 0.0904 0.037 0.0155 0.0651 0.3731 0.4743 0.0684 0.0292 0.0787 0.0292 0.206 0.233 0.0856
Nucleolus 0.0173 0.0264 0.0162 0.0024 0.0038 0.0368 0.02 0.0162 0.0202 0.0032 0.0256 0.0289 0.0105 0.0072 0.0109 0.0192 0.0457 0.0146
Nucleus 0.8228 0.9032 0.9096 0.6305 0.4393 0.8681 0.8517 0.9672 0.8486 0.5719 0.4102 0.8864 0.8709 0.9115 0.9116 0.3943 0.6207 0.8866
Peroxisomes 0.0052 0.013 0 0 0.0015 0 0.0054 0 0.005 0.001 0.0003 0.0002 0.0122 0 0.0024 0.0173 0.0005 0.001
Punctate Nuclear 0.0178 0.0008 0.0012 0.0012 0.0131 0.0004 0.0142 0.0006 0.0033 0.0022 0.0064 0.0027 0.0093 0.0002 0.0014 0.0154 0.0345 0.0005
Vacuole 0.0009 0.0002 0.0001 0.0005 0.001 0.0004 0.0021 0 0.0033 0.0011 0.0057 0.001 0.0066 0.0002 0.0011 0.0058 0.0148 0.0004
Vacuole Periphery 0.0024 0.001 0.0009 0.0065 0.0072 0.0021 0.0019 0.0001 0.0019 0.0073 0.0128 0.0026 0.0019 0.0013 0.0016 0.0067 0.0184 0.0025

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 36.7281 12.5742 8.6394 23.4021 27.3559 36.4474 9.8885 7.4686 15.4283 32.3779
Translational Efficiency 1.6934 0.7447 1.3134 0.8732 1.4955 1.9626 0.9753 1.0228 0.9366 1.2628

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2341 473 478 1030 2136 1285 1078 67 4477 1758 1556 1097

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 727.41 923.22 1159.59 1030.19 821.96 836.59 910.28 1119.20 772.52 859.90 986.87 1035.63
Standard Deviation 97.19 138.08 165.56 159.92 109.54 138.29 201.28 186.59 113.55 143.47 222.97 163.08
Intensity Change Log 2 — 0.343906 0.672774 0.502070 — 0.025453 0.147242 0.445328 — 0.183738 0.417858 0.472246

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000198 0.000491 0.001228 0.000805 0.000253 0.000467 0.000733 0.001503 0.000224 0.000474 0.000885 0.000848
Bud Neck 0.003498 0.004774 0.014396 0.021448 0.003374 0.006046 0.006948 0.024310 0.003439 0.005704 0.009236 0.021623
Bud Site 0.001003 0.000499 0.000978 0.003271 0.000651 0.001827 0.001765 0.003323 0.000835 0.001470 0.001523 0.003274
Cell Periphery 0.000159 0.000070 0.000052 0.000086 0.000122 0.000182 0.000251 0.000062 0.000142 0.000152 0.000190 0.000085
Cytoplasm 0.025077 0.002745 0.001031 0.009989 0.003466 0.004743 0.011506 0.004562 0.014766 0.004205 0.008288 0.009657
Cytoplasmic Foci 0.002142 0.000302 0.000079 0.001203 0.000233 0.000964 0.000633 0.000221 0.001231 0.000786 0.000463 0.001143
Eisosomes 0.000014 0.000017 0.000037 0.000014 0.000013 0.000020 0.000027 0.000020 0.000014 0.000019 0.000030 0.000015
Endoplasmic Reticulum 0.005932 0.013370 0.011743 0.009454 0.011382 0.013347 0.008680 0.012220 0.008532 0.013353 0.009621 0.009623
Endosome 0.000776 0.000508 0.000539 0.003565 0.000462 0.001189 0.000489 0.001800 0.000626 0.001006 0.000504 0.003457
Golgi 0.000426 0.000418 0.000117 0.003114 0.000050 0.000305 0.000132 0.000164 0.000247 0.000335 0.000127 0.002934
Lipid Particles 0.001092 0.000174 0.000179 0.000234 0.000383 0.000793 0.000206 0.000423 0.000754 0.000626 0.000198 0.000245
Mitochondria 0.002326 0.002363 0.002961 0.007580 0.001576 0.003286 0.001789 0.006309 0.001968 0.003038 0.002149 0.007503
Mitotic Spindle 0.001487 0.001665 0.001071 0.035803 0.000671 0.002137 0.002395 0.055734 0.001098 0.002010 0.001988 0.037021
None 0.018089 0.002985 0.000864 0.002762 0.002839 0.004711 0.032419 0.002115 0.010813 0.004247 0.022725 0.002723
Nuclear Periphery 0.036610 0.041738 0.064624 0.231305* 0.042064 0.047970 0.045503 0.164463* 0.039212 0.046293 0.051377 0.227223*
Nuclear Periphery Foci 0.004722 0.002599 0.005874 0.047272 0.002873 0.005496 0.004740 0.031455 0.003840 0.004716 0.005088 0.046306
Nucleolus 0.059072 0.029389 0.031016 0.030848 0.029539 0.035155 0.017101 0.048679 0.044982 0.033604 0.021376 0.031937
Nucleus 0.835322* 0.889196* 0.858381* 0.573849* 0.898481* 0.867828* 0.861525* 0.635167* 0.865456* 0.873577* 0.860559* 0.577594*
Peroxisomes 0.000501 0.000449 0.001015 0.000309 0.000242 0.000552 0.000270 0.000469 0.000378 0.000524 0.000499 0.000319
Vacuole 0.001103 0.002060 0.002952 0.010669 0.000871 0.001802 0.002500 0.005012 0.000992 0.001871 0.002639 0.010323
Vacuole Periphery 0.000450 0.004188 0.000860 0.006419 0.000456 0.001181 0.000390 0.001987 0.000453 0.001990 0.000534 0.006149

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.41 -17.10 -15.25 -7.34 4.26 -5.86 -12.75 -2.96 -2.58 -2.00 -8.57 -20.17 -13.99 -8.99 -1.26
Bud Neck -1.23 -9.29 -12.49 -10.94 -5.62 -2.94 -7.07 -3.72 -3.28 -2.80 -3.14 -11.02 -13.14 -11.45 -8.75
Bud Site 1.58 -0.63 -3.16 -3.97 -3.08 -2.04 -3.01 -1.97 -1.13 -1.09 -1.47 -2.76 -3.68 -2.57 -2.43
Cell Periphery 4.37 9.03 5.29 -1.26 -5.17 -1.22 -2.83 2.65 2.39 4.46 -0.31 -1.39 4.36 1.74 3.45
Cytoplasm 11.71 14.45 8.13 -4.14 -6.74 -1.43 -4.49 -1.07 -0.53 1.58 9.55 4.87 4.28 -3.62 -0.57
Cytoplasmic Foci 4.68 5.56 3.25 -1.75 -2.99 -2.60 -1.95 0.52 2.71 2.10 1.49 3.09 1.83 0.30 -0.91
Eisosomes -2.01 -11.21 -5.29 -1.68 7.94 -4.74 -6.57 -4.01 -2.12 0.02 -4.78 -10.30 -7.01 -1.51 5.33
Endoplasmic Reticulum -4.18 -5.76 -5.58 2.23 1.87 -1.84 3.52 -0.30 0.82 -2.03 -5.59 -1.50 -1.57 4.16 -0.16
Endosome 1.66 2.02 -3.59 -4.03 -4.10 -2.47 -0.50 -2.73 -0.80 -2.65 -1.68 1.92 -4.03 -3.01 -4.30
Golgi 0.03 1.81 -1.84 -1.75 -2.23 -1.73 -1.94 -3.27 0.94 -0.57 -0.52 1.28 -2.07 -1.94 -2.22
Lipid Particles 5.44 5.47 5.16 -1.35 -1.51 -1.26 1.54 -0.66 0.78 -1.74 0.51 5.39 4.85 1.65 -1.54
Mitochondria -0.04 -1.08 -4.07 -3.73 -3.92 -2.16 -0.79 -3.97 -2.21 -3.85 -1.68 -0.71 -4.99 -3.52 -4.97
Mitotic Spindle -0.15 0.43 -8.25 -7.84 -8.32 -1.46 -2.48 -2.27 -2.19 -2.13 -1.09 -1.97 -8.67 -8.34 -8.26
None 7.98 12.01 10.48 0.13 -6.42 -2.08 -7.14 1.28 2.73 7.34 6.16 -3.46 9.77 2.04 6.87
Nuclear Periphery -0.71 -1.93 -12.85 -10.48 -8.94 -1.01 0.44 -2.38 -2.15 -2.45 -1.58 -0.95 -13.05 -11.57 -11.75
Nuclear Periphery Foci 2.24 0.00 -8.82 -9.44 -8.59 -2.51 -1.20 -2.04 -1.75 -1.90 -1.03 -0.54 -9.40 -8.95 -9.10
Nucleolus 6.81 6.08 5.94 -1.29 -0.61 -1.70 5.09 -1.63 -1.26 -2.46 4.49 10.00 2.68 -0.82 -4.29
Nucleus -5.12 -3.12 14.19 15.68 14.07 3.80 3.51 4.80 4.06 4.09 -1.41 -0.67 17.74 17.38 16.87
Peroxisomes 0.26 -2.13 1.10 1.82 5.23 -2.66 -0.83 -1.51 0.16 -1.32 -0.92 -1.05 0.70 2.35 3.54
Vacuole -1.11 -4.84 -6.60 -5.39 -5.27 -2.33 -3.79 -1.74 -1.65 -1.57 -2.43 -5.17 -6.82 -6.17 -5.58
Vacuole Periphery -1.72 -2.80 -4.64 -0.14 -4.26 -2.83 1.32 -2.60 -0.73 -2.84 -2.50 -0.52 -4.71 -2.28 -4.67
Standard name
Human Ortholog
Description B subunit of DNA polymerase alpha-primase complex; required for initiation of DNA replication during mitotic and premeiotic DNA synthesis; also functions in telomere capping and length regulation
Localization
Cell Percentages nucleus (82%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Pol12

Pol12


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pol12-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available